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JP3394017B2 - Extraction method of mushroom extract - Google Patents
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JP3394017B2 - Extraction method of mushroom extract - Google Patents

Extraction method of mushroom extract

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Publication number
JP3394017B2
JP3394017B2 JP2000083110A JP2000083110A JP3394017B2 JP 3394017 B2 JP3394017 B2 JP 3394017B2 JP 2000083110 A JP2000083110 A JP 2000083110A JP 2000083110 A JP2000083110 A JP 2000083110A JP 3394017 B2 JP3394017 B2 JP 3394017B2
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JP
Japan
Prior art keywords
extract
water
agaricus
extraction method
extraction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP2000083110A
Other languages
Japanese (ja)
Other versions
JP2001258496A (en
Inventor
龍也 神田
Original Assignee
ユウキ株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ユウキ株式会社 filed Critical ユウキ株式会社
Priority to JP2000083110A priority Critical patent/JP3394017B2/en
Publication of JP2001258496A publication Critical patent/JP2001258496A/en
Application granted granted Critical
Publication of JP3394017B2 publication Critical patent/JP3394017B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Preparation Of Fruits And Vegetables (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Extraction Or Liquid Replacement (AREA)

Description

【発明の詳細な説明】 【0001】 【発明の属する技術分野】本発明は、乾燥状態のアガリ
クスブラジィムリル(以下、アガリクスと略記する)か
らエキスを抽出する方法に関するものである。 【0002】 【従来の技術】従来、乾燥状態のアガリクスからエキス
を抽出するに際しては、熱水抽出法、いわゆる煎じる方
法が一般的に最も多く利用されている。 【0003】 【発明が解決しようとする課題】従来の技術で述べた熱
水抽出法では、加熱して熱水による抽出を行って、β−
グルカンなどを主とする有効成分を一定量抽出すること
が可能ではあるが、それと同時にキノコ自体が本来含有
するカドミウム、ヒ素、重金属なども同時にエキス中に
抽出してしまうという問題点があった。参考として、乾
燥アガリクスを検体とした分析結果の一例を表1に示
す。 【0004】 【表1】【0005】 【課題を解決するための手段】上記の問題点を解決する
ために、本発明は、特殊な前処理を実施することによ
り、β−グルカンなどの有効成分は熱水抽出法と変わり
なく抽出しながら、不純物であるカドミウム、ヒ素、重
金属などは大幅に低減することができるようにしたもの
である。 【0006】 【発明の実施の形態】本発明によるキノコエキスの抽出
方法は、基本的に、アガリクス乾燥体をあらかじめ水と
アルコールを混合した水に漬け、アガリクスの内部に水
分が十分に含まれた状態にした後、冷凍凍結と解凍を繰
り返すことにより組織内部での細胞破壊を繰り返し行っ
てから、冷水に漬けてエキスを抽出することを特徴とし
ておりこれによって、有効成分のみを効率よく抽出でき
るものである。 【0007】本発明をさらに具体的に説明すると以下の
ようである。 【0008】まず最初に、原料であるアガリクス乾燥体
をあらかじめ水とアルコールを混合した水に漬けること
で、アガリクスの内部に水分が十分に含まれた状態にす
る。具体的には、乾燥キノコの重量が30%増量する程
度の水分をキノコに与えるようにするのが好ましい。水
にアルコールを加えるのは、変敗を防ぐためと、その後
の抽出をより効果的に行うためである。 【0009】次に、水分を加えたアガリクス乾燥体の温
度を徐々に下げ、−20℃近辺で一度冷凍凍結する。こ
のとき、キノコ本体内に含まれた水分が凍結する際に、
組織内部で膨張して細胞を一部破壊する。 【0010】その後、一旦解凍してから、再度冷凍凍結
することにより、繰り返し組織内部での細胞破壊を行
う。本発明では、冷凍凍結と解凍の繰り返しの回数に特
に限定はなく、エキスの抽出濃度と作業性を考慮して適
宜決めればよい。 【0011】俗に、食品、特に肉や魚を解凍した後で再
び冷凍すると味が大幅に落ちるというのは、それらの組
織細胞が破壊され、味が損なわれるもので、本発明では
それを逆に利用して細胞組織内の有効成分を取り出そう
とするものである。 【0012】上記のように冷凍と解凍を繰り返し行った
後、冷水に漬け、熱を加えずにエキスを抽出する。この
場合、溶液への固形分の溶解速度を分析すると、摂氏1
0℃の水では、24時間で成分抽出がほぼピークをむか
え、その後24時間放置しても、溶液中の固形分量は変
化のないことから、24時間が最も効率的な抽出時間と
考えられる。 【0013】 【実施例】アガリクス乾燥体を水とアルコールの混合水
に漬けて重量が30%増量する程度の水分をキノコに与
えた後、徐々に温度を下げて−20℃近辺で冷凍凍結さ
せた。その後、一旦解凍してから、また再度冷凍凍結さ
せてから、摂氏10℃の冷水に24時間漬けてエキスを
抽出した。 【0014】そのエキスを検体として不純物を分析した
結果を表2に示す。この表2から分かるように、ヒ素お
よび重金属は検出されず、カドミウムは0.06ppm
であった。カドミウムのWHO(世界保健機構)での適
格基準は0.2ppmであるので、この規格に照らして
も1/3以下の値におさまっている。 【0015】 【表2】 【0016】比較例として、一般的な熱水抽出法でエキ
スの抽出を行った。ここでは、実施例で用いたのと同量
の水で同量のアガリクスを熱水抽出した。そのエキスを
検体として不純物を分析した結果は表3に示すとおりで
あり、ヒ素0.3ppm、重金属1.1ppm、カドミ
ウム0.14ppmとなっており、本発明の抽出方法の
優位性が立証されるものである。 【0017】 【表3】【0018】そして、実施例においては、上記のように
不純物が低減されながらも、有効成分の代表であるβ−
グルカンは1.6g/100g(分析方法:酵素法)含
まれており、比較例の熱水抽出で得られた1.3g/1
00g(分析方法:酵素法)よりもかえって多く得るこ
とができた。 【0019】 【発明の効果】以上説明したように、本発明によれば、
有効成分を効率よく抽出しながら、不純物は極力抽出を
おさえることができる。したがって、食品加工の各分野
への応用を拡大することが可能となる。
Description: BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for extracting an extract from agaricus bradymril (hereinafter abbreviated as agaricus) in a dry state. 2. Description of the Related Art Conventionally, when extracting an extract from agaricus in a dry state, a hot water extraction method, a so-called decoction method, is generally most often used. [0003] In the hot water extraction method described in the prior art, β-β is extracted by heating and extracting with hot water.
Although it is possible to extract a certain amount of an active ingredient mainly including glucan, there is a problem that cadmium, arsenic, heavy metals and the like originally contained in the mushroom itself are simultaneously extracted into the extract. For reference, an example of an analysis result using dried agaricus as a sample is shown in Table 1. [Table 1] [0005] In order to solve the above-mentioned problems, the present invention carries out a special pretreatment, whereby the active ingredient such as β-glucan can be replaced with a hot water extraction method. Cadmium, arsenic, heavy metals, etc., which are impurities, can be greatly reduced while extraction is not performed. DETAILED DESCRIPTION OF THE INVENTION The method for extracting a mushroom extract according to the present invention is basically such that a dried agaricus body is immersed in water in which water and alcohol are mixed in advance, and a sufficient amount of water is contained in the agaricus. After being in a state, repeated freeze-freezing and thawing repeatedly repeats cell destruction inside the tissue, and then immerses in cold water to extract the extract, which enables efficient extraction of only the active ingredient. It is. The present invention will be described more specifically as follows. First, a dried agaricus material, which is a raw material, is immersed in water in which water and alcohol are mixed in advance, so that a sufficient amount of water is contained in the agaricus. Specifically, it is preferable to give water to the mushroom such that the weight of the dried mushroom increases by 30%. The addition of alcohol to the water is to prevent spoilage and to make the subsequent extraction more effective. Next, the temperature of the dried Agaricus body to which water has been added is gradually lowered, and frozen and frozen once at about -20 ° C. At this time, when the water contained in the mushroom body freezes,
It swells inside the tissue and partially destroys the cells. [0010] Thereafter, the cells are once thawed and then frozen and frozen again, thereby repeatedly destroying the cells inside the tissue. In the present invention, the number of repetitions of freeze-freezing and thawing is not particularly limited, and may be appropriately determined in consideration of the extraction concentration of extract and workability. In general, when food, especially meat or fish is thawed and then frozen again, the taste is greatly reduced because the tissue cells are destroyed and the taste is impaired. To take out the active ingredient from the cell tissue. After repeated freezing and thawing as described above, the extract is immersed in cold water and the extract is extracted without applying heat. In this case, analyzing the dissolution rate of the solid in the solution,
In the case of water at 0 ° C., component extraction almost reaches a peak in 24 hours, and even if left for 24 hours, the amount of solids in the solution does not change. Therefore, 24 hours is considered to be the most efficient extraction time. EXAMPLE A dried agaricus product was immersed in a mixture of water and alcohol to give water to the mushroom so that the weight increased by 30%. Was. Thereafter, the extract was once thawed, frozen and frozen again, and then immersed in cold water at 10 ° C. for 24 hours to extract the extract. Table 2 shows the results of analysis of impurities using the extract as a sample. As can be seen from Table 2, arsenic and heavy metals were not detected, and cadmium was 0.06 ppm.
Met. The qualification standard of cadmium in the WHO (World Health Organization) is 0.2 ppm, so that it is less than 1/3 in light of this standard. [Table 2] As a comparative example, the extract was extracted by a general hot water extraction method. Here, the same amount of agaricus was extracted with hot water using the same amount of water used in the examples. The result of analyzing impurities using the extract as a sample is as shown in Table 3, which is 0.3 ppm of arsenic, 1.1 ppm of heavy metals, and 0.14 ppm of cadmium, demonstrating the superiority of the extraction method of the present invention. Things. [Table 3] In the embodiment, while the impurities are reduced as described above, β-
Glucan is contained in 1.6 g / 100 g (analytical method: enzymatic method), and 1.3 g / 1 obtained by hot water extraction of the comparative example.
More than 100 g (analytical method: enzyme method) could be obtained. As described above, according to the present invention,
The impurities can be extracted as little as possible while efficiently extracting the active ingredient. Therefore, it is possible to expand applications to various fields of food processing.

───────────────────────────────────────────────────── フロントページの続き (58)調査した分野(Int.Cl.7,DB名) A23L 1/212 - 1/218 A23L 1/27 - 1/308 JICSTファイル(JOIS)──────────────────────────────────────────────────続 き Continued on the front page (58) Field surveyed (Int.Cl. 7 , DB name) A23L 1/212-1/218 A23L 1/27-1/308 JICST file (JOIS)

Claims (1)

(57)【特許請求の範囲】 【請求項1】 アガリクス乾燥体をあらかじめ水とアル
コールを混合した水に漬け、アガリクスの内部に水分が
十分に含まれた状態にした後、冷凍凍結と解凍を繰り返
すことにより組織内部での細胞破壊を繰り返し行ってか
ら、摂氏10℃の冷水に漬けてエキスを抽出することを
特徴とするキノコエキスの抽出方法。
(57) [Claims] [Claim 1] A dried agaricus body is immersed in water in which water and alcohol are mixed in advance, and the inside of the agaricus is sufficiently contained in water. A method for extracting a mushroom extract, comprising repeating cell destruction within a tissue by repeating the process, and then immersing the extract in cold water at 10 ° C. to extract the extract.
JP2000083110A 2000-03-24 2000-03-24 Extraction method of mushroom extract Expired - Lifetime JP3394017B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2000083110A JP3394017B2 (en) 2000-03-24 2000-03-24 Extraction method of mushroom extract

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2000083110A JP3394017B2 (en) 2000-03-24 2000-03-24 Extraction method of mushroom extract

Publications (2)

Publication Number Publication Date
JP2001258496A JP2001258496A (en) 2001-09-25
JP3394017B2 true JP3394017B2 (en) 2003-04-07

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5142116B2 (en) * 2005-06-30 2013-02-13 国立大学法人 琉球大学 Health food using papaya seeds
CN115024486B (en) * 2022-05-12 2023-08-22 华中农业大学 Pleurotus eryngii vegetarian abalone meat and processing method thereof
KR102470454B1 (en) * 2022-05-31 2022-11-25 주식회사 뷰니스 Health functional food composition for anti-obesity comprising gynostemma pentaphyllum leaf extract and complex extract, and manufacturing method thereof

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