JP3483741B2 - Partially decomposed psyllium product and method for producing the same - Google Patents

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a psyllium moiety having a bowel movement-improving effect obtained by reacting psyllium with an enzyme selected from the group consisting of pectinases having psyllium-degrading activity, hemicellulases, and combinations thereof. The present invention relates to a decomposed product, a method for producing the same, a bowel movement improving agent containing the partially decomposed product, and a beverage and a food to which the partially decomposed product is added.

[0002]

BACKGROUND OF THE INVENTION Until recently, dietary fiber was not utilized in the body and was nutritionally ignored, but it has been proved that dietary fiber is effective in preventing various diseases. The importance of its intake has come to be recognized.
The effects of ingestion include an effect of improving bowel movements (improvement of constipation and diarrhea), an effect of suppressing absorption of cholesterol, an effect of suppressing an increase in blood sugar level, and the like, which is also effective in preventing obesity, diabetes, colorectal cancer, etc. It is being appreciated.

As a method of ingesting dietary fiber, active intake of vegetables, root vegetables and the like is desired, but it is often deficient in modern people's dietary habits, and various forms of supplementary foods are ingested. Are manufactured and sold. The most popular among them is to drink a liquid beverage containing water-soluble dietary fiber such as indigestible dextrin. However, in the case of expecting a bowel movement improving effect, the effect of indigestible dextrin cannot be said to be sufficient.

At present, psyllium is known as a dietary fiber that is most expected to improve bowel movements. For example, ACS
Symposium Series (American Chemical Society), No.21
4, P61-70,1983 show that psyllium increases the amount of feces (wet weight) most and shortens the bowel movement time compared to cellulose and various brans. However, when trying to apply psyllium to liquid beverages, when dissolved in water, psyllium contains 50-100 times more water (when put in water and well stirred to form a uniform dispersion), and is highly viscous. It is not suitable for liquid drinks because it forms a gel and becomes syrup-like. Therefore, it is common to use powders or granules of commercially available psyllium drugs immediately before ingestion before they are suspended in water and before gelation, or in accordance with a powdered drug. , In this case, there is a feeling of strangeness such as complexity of drinking, stickiness in the oral cavity during or after ingestion,
In addition, there were problems such as the avoidance of pharmaceutical intake methods.

Therefore, in order to make psyllium into the form of a general liquid beverage that is most easily ingested, it is conceivable to reduce the viscosity of the psyllium dispersion. The method of making In fact, acid-slightly decomposed psyllium is available on the market, but not so low viscosity that it can be applied as a liquid beverage. It is considered that this is because if the acid decomposition is advanced to such an extent that it can be applied as a liquid beverage, the effect of improving the bowel movement originally possessed by psyllium is extremely lowered.

[0006]

Therefore, it has a high effect of improving bowel movements and is desirable as a liquid beverage when dissolved in water (including hydration and uniform dispersion, the same applies hereinafter). As described above, the subject of the invention is to obtain a substance having a low viscosity and to be drinkable.

[0007]

[Means for Solving the Problems] Psyllium has xylan as a main chain and has a highly branched hemicellulose structure.
The side chains are arabinose, xylose, galacturonic acid,
It contains many constituent sugars such as rhamnose. When the sugar chain structure of a general polysaccharide is decomposed under acidic conditions, various constituent sugars are cleaved to lower the molecular weight. Therefore, it is considered that even when the psyllium is decomposed, the molecular weight is reduced and the viscosity is lowered. Then, the present inventors first added various acids such as citric acid and hydrochloric acid so that psyllium can be used as a liquid beverage, and produced a substance that was partially decomposed by heating. However, when the effect of improving the bowel movement of the partially decomposed product by this acid (the amount of increased feces in the rat) was measured, the effect was remarkably reduced, and when it was decomposed to a viscosity suitable for drinking, it became water-soluble dietary fiber (indigestible dextrin). Only the same or less effect was obtained. This may be because the decomposition by acid randomly acts on the cleavage of the sugar chain structure and the control of the cleavage site is not easy, so it is difficult to obtain a degradation product having an appropriate chain length. it was thought.

[0008] Therefore, the present inventors have studied a method of partial decomposition using several enzymes, but psyllium has high resistance to the enzymes, and it is easy to obtain an enzymatic decomposition product having a viscosity suitable for a liquid beverage. It wasn't. But,
The partial decomposition product of psyllium partially decomposed by the action of an enzyme selected from the group consisting of pectinase, hemicellulase and combinations thereof to a drinkable viscosity is higher than that of the partial decomposition product of psyllium obtained by decomposition with acid. The present invention has been completed by finding that the improvement effect remains. In particular, it is preferable to use pectinase as the enzyme, and when hemicellulase and pectinase are mixed and used, it is more effective if the pectinase is mixed at an enzyme titer of 50% or more. It was also found that by adding ascorbic acid during or after the decomposition reaction, the viscosity can be more effectively reduced and the discoloration of the solution can be prevented.

That is, the present invention provides a psyllium partial fraction obtained by reacting psyllium with an enzyme selected from the group consisting of pectinase having psyllium-degrading activity, hemicellulase, and combinations thereof.
An aqueous solution containing 4% by weight of the partially decomposed product
(The term "aqueous solution" is used herein to exactly dissolve in water.
Even if it is not in solution, it hydrates and disperses evenly
Also, the viscosity of a liquid in a clear state) is B
-Type viscometer, 4 ° C, 60 rpm , pH about 4
, Which is 30-200 cP and has a bowel movement improving effect.
It is a partially decomposed product of lithium . Furthermore, the present invention relates to a method for producing the partially decomposed product, a bowel movement improving agent containing the partially decomposed product, or a liquid beverage or food to which the agent is added. Further, more preferably, ascorbic acid is added during the production process of these partially decomposed psyllium products.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION The psyllium used in the present invention is a polysaccharide obtained from the seed coat of the plant of Plantago ovata of the plantain family, and commercially available psyllium and psyllium seed gum can be used. It is preferable that the particle size is not so large and that the seed shells are less mixed. In the present invention, it is possible to use, as a raw material, a material obtained by slightly treating psyllium with acid or heating to slightly reduce the original viscosity of psyllium. In the case of the present invention, when the one which is decomposed excessively by an acid or heating is used, the effect of improving the bowel movement of the partially decomposed product to be obtained may be lowered, and the desired effect may not be obtained, but it is not decomposed. If the viscosity is slightly lowered from that of psyllium, the effect is not significantly reduced. Therefore, in the present invention, it is possible to use as a raw material even psyllium that has been treated to this extent, rather by using such a raw material, the viscosity of the final product can be lowered to some extent,
It may be valid.

In order to prepare the partial decomposition product of psyllium of the present invention, first, this psyllium is added to water and the enzyme is allowed to act with stirring. Regarding the method of addition, it is preferable to add psyllium and the like to water and then add psyllium so that the enzyme and the like can act uniformly, but psyllium may be added first. If the addition amount of psyllium is too large, the viscosity becomes extremely high and stirring becomes difficult. Therefore, it is usually preferable to set the addition amount to 10% or less by weight ratio to water.

The enzyme used in the present invention is an enzyme selected from the group consisting of pectinase having psyllium-degrading activity, hemicellulase, and a combination thereof, preferably pectinase. The reason why pectinase is advantageous is that, as shown in the experimental results described later, when comparing the viscosities of partially decomposed psyllium products having almost the same bowel movement-improving effect, it was found that decomposition was performed using pectinase. This is because it is possible to obtain one having a lower viscosity.

The amount of enzyme added is usually 0.5 in terms of the total amount of enzyme titer to psyllium per 1 g of psyllium.
-6 U, preferably 1-3 U, is preferable, but if the concentration is low, the decomposition does not proceed, and conversely, if the concentration is high, the enzyme is useless, so it is appropriately adjusted.

Since some pectinases do not act on psyllium depending on their origin, it is preferable to select them based on the psyllium-decomposing ability, and those with high psyllium-decomposing activity are preferable. As a result of examination by the applicant, Aspe
It has been clarified that an enzyme derived from the genus rgillus is desirable, and the specific trade name of the enzyme is (pectinase G
"Amano"; Amano Pharmaceutical Co., Cellulosin PE60; Hankyu Kyoei Bussan) and the like can be used. Regarding hemicellulase, there is a considerable difference in enzyme activity depending on the composition of the enzyme and the like, and it is preferable to select a hemicellulase having high psyllium-decomposing activity. Specifically (cellulosin HC100
(Product name); Hankyu Kyoei Bussan) etc. are good. In any of the enzymes, the point is that they have an enzymatic activity of decomposing psyllium, and as an addition amount thereof, an enzyme titer of usually about 0.5 to 6 U may be used with respect to 1 g of psyllium (titer of titer). For the measuring method, see the examples of the present specification).

The partially decomposed psyllium of the present invention is obtained by partially decomposing psyllium with the above-mentioned enzyme. The viscosity of a 4% by weight aqueous solution of this is 4 ° C., 60 rpm, with a B type viscometer. The pH is about 200 cP or less, preferably about 180 cP, which is about the level of tomato juice, when measured under the measurement conditions of pH about 4. However, if the decomposition proceeds too much,
Since the effect of improving bowel movements cannot be obtained, the viscosity of a 4% by weight aqueous solution does not fall below about 50 cP under the above-mentioned conditions, as far as psyllium is partially decomposed with an enzyme according to the method of the present invention. Is desirable. However, in the case of the present invention, it is possible to further reduce the viscosity by adding ascorbic acid to the enzyme reaction solution or by adopting a method such as temporarily freeze-drying the partially decomposed product,
With the addition of these techniques, it is possible to finally use a material having a viscosity of about 30 cP. By the way, the viscosity of commercially available nectar is about 70 cP, and the viscosity of tomato juice is about 180 cP.

Here, the reason for defining the preferred partial decomposition product of psyllium of the present invention with the viscosity of a 4% by weight aqueous solution is that the usual drinking concentration when the liquid beverage according to the present invention is prepared using the partial decomposition product. This is because of consideration.
In the present invention, partial decomposition reduces the bowel movement-improving effect more than undecomposed psyllium, though not as much as the acid decomposition product. The reduction rate of the effect was about 50% of the original effect of psyllium from the experimental results of the present inventors.
On the other hand, the amount of the beverage that an adult normally takes at one time is generally about 200 ml. Regarding the daily intake of undegraded psyllium that can be expected to be effective, the research report by Nissin Foods Co., Ltd. (November 1996, psyllium intake test) submitted to the Ministry of Health as an application document for food for specified health uses It is known that the data is about 4 g. Therefore, the daily requirement of 4 g of undecomposed psyllium to obtain the desired effect is about 2% by weight in the solution when added to 200 ml of water. In the case of the partially decomposed psyllium product of the present invention, its effect is about 50% of that of undecomposed psyllium, as will be described later.
It is necessary to add wt%. That is, the concentration of 200 ml of the liquid beverage of the present invention at the time of drinking needs to be about 4% by weight, which is the reason why the viscosity of a 4% by weight aqueous solution is particularly specified. And, as mentioned earlier, 4
The viscosity of the wt% aqueous solution is a drinkable viscosity, that is, about 20
It must be 0 cP or less. Although 200 cP is somewhat difficult to drink, its viscosity can be slightly lowered by techniques such as freeze-drying and spray-drying and techniques such as addition of ascorbic acid, so it is within the usable range. By the way, the viscosity of an aqueous solution of 2% by weight of undecomposed psyllium is 100,000.
It is more than cP and not suitable for drinking.

As a reaction condition between the enzyme and psyllium, p
H can be about 3 to 8, but it is preferable to set it to about pH 4 which is the optimum reaction pH of the enzyme to be added. For adjusting the pH, it is preferable to add an organic acid such as citric acid or lactic acid that can be used in foods, but hydrochloric acid or the like can also be used. The reaction temperature may be room temperature to 70 ° C., but it is preferably higher to some extent in view of the reaction efficiency of the enzyme. However, if the temperature is too high, not only the inactivation of the enzyme but also the problem that the solution is colored occurs. Therefore, it is preferably carried out at 40 to 50 ° C. The reaction time is, for example, 5 to 48 hours. If the reaction is carried out for a long time, not only the enzymatic reaction will not proceed any further, but also the problem of contamination by bacteria or the like may occur. During the reaction, it is better to carry out stirring with as strong a shearing force as possible so as to allow the contact between psyllium and the enzyme, and it is better to use a homomixer or the like having a larger convection than the propeller type stirring method. However, if stirring is performed such that air is included, the solution may be colored.

The coloring of the solution causes the reaction solution to turn gray or brown in the course of enzymatically decomposing psyllium, resulting in turbidity, which lowers the commercial value of the partially decomposed product obtained. In order to prevent the occurrence of coloring or turbidity, it is preferable to keep the reaction solution out of contact with air as much as possible and to add an antioxidant such as ascorbic acid to the solution. When ascorbic acid is added to the solution, the amount of ascorbic acid is 0, based on the weight of psyllium added.
It is preferably about 5 to 5% by weight, which makes it possible to suppress the color tone to pale yellow. Further, the viscosity of the partially decomposed product can be reduced by adding ascorbic acid. As is clear from the experimental results of the present invention, since the effect of improving bowel movement does not decrease even if the viscosity is decreased by the addition of ascorbic acid, it is understood that the effect of ascorbic acid addition is higher if the viscosity is the same, Also in this respect, addition of ascorbic acid is advantageous for the present invention. Ascorbic acid may be added either before it is added to water before the start of the enzymatic reaction, when it is added to the reaction system during the addition during the enzymatic reaction, or before heating to deactivate the enzyme. You may add it. In short, if ascorbic acid is added before inactivating the enzyme, the viscosity of the partially decomposed product can be suppressed to a considerably low value at any time. However, especially in view of prevention of the above-mentioned coloration, it is preferable to add them as early as possible, and it is most effective to add them before the start of the enzyme reaction.

Further, the coloring of the solution after the enzymatic reaction can be decolorized by using a bleaching agent which can be usually used for foods and the like. For example, coconut powder, nitrite, etc. may be added.

Pyrium is decomposed according to the above-mentioned enzyme reaction conditions. However, due to some differences in the conditions, the decomposition may proceed too far and the effect may not be obtained, or the decomposition may be insufficient and the viscosity may be too high. It is desirable to appropriately adjust the conditions according to the psyllium-decomposing activity, reaction temperature, and the like. Therefore, preferably, the reaction conditions are adjusted while continuously monitoring the viscosity of the reaction solution.

After the reaction has proceeded to a desired degree, the reaction is terminated by heating to prevent the further decomposition and deactivating the enzyme. The heating conditions are, for example, 85 ° C. and 15 minutes or more. Before heating, add acid such as citric acid,
It is desirable to reduce H to about 4. This ensures the deactivation of the enzyme, the prevention of coloration and the bactericidal effect of the bacteria in the solution.

The aqueous solution containing the partially decomposed psyllium thus obtained can be drunk as it is and taken as a bowel movement improving agent or a soft drink. In that case,
The concentration of solution, dose, etc. should be adjusted in consideration of the degree of desired effect. Examples of the adjusting method include dilution with water and the like, and concentration by various methods such as concentration under reduced pressure. Since the partial decomposition product of the present invention is considered to have an effect of improving bowel movements by about 40 to 60% as compared with undecomposed psyllium, as described above, ingestion of a liquid beverage having a volume of 200 ml once a day Then, it is preferable that the concentration of the partial decomposition product of psyllium of the present invention is about 4 to 6% by weight. When preparing such a partially decomposed product into a beverage, a sweetener,
The taste may be adjusted by adding fruit juice or the like. In addition, additives such as coloring agents, flavoring agents, and preservatives that can be added to ordinary beverages can be added as desired.

The partial decomposition product of psyllium of the present invention is
It is also possible to process it into a powder or solid by a method such as freeze-drying or spray-drying, and it is also possible to reduce the viscosity of the aqueous solution when it is once made into a powder and dispersed or hydrated again in water.

It is also possible to mix the psyllium partially decomposed product of the present invention with foods other than beverages, such as snacks, jelly-like confections, noodles and the like. In the case of the product of the present invention,
Since the viscosity of the aqueous solution is much lower than that of psyllium as it is, there are advantages that the processing operation is easy and the influence on the texture is small.

[0025]

EXAMPLES The present invention will be specifically described below, but the present invention is not limitedly interpreted based on these examples.

Hereinafter, in the examples, the viscosity of the sample is measured at 4 ° C. when the product of the present invention is used as a beverage.
This is in consideration of the fact that a chilled product is drunk like a normal soft drink. Moreover, the reason that the bowel movement improving effect is measured by the amount of increased feces is that bowel movements are most affected by the amount of feces, and it is considered that increasing the amount of feces directly leads to the improvement of constipation.

First, the effects of improving the viscosity and bowel movement of each of the partially decomposed psyllium of the present invention, the partially decomposed psyllium by an acid, the indigestible dietary fiber and the like were compared.

Example 1 0.2 g of citric acid in 1 L of water,
The pH was added ascorbic acid 1g was adjusted to 3.6, Aspe
4 g of pectinase derived from genus rgillus (trade name: pectinase G "Amano"; Amano Pharmaceuticals, enzyme titer against psyllium: 24.6 U / g, this activity is described later) was added. To this, 50 g of psyllium powder (trade name: Psyllium Seed Gum Food Made P-100; Shikibo) was added and mixed, and stirred at 45 ° C. for 16 hours (DCSTIRRER DCL-R;
The enzyme was made to act with stirring by Tokyo Rika Kikai. After the reaction, 2.8 g of citric acid was further added to adjust the pH to 3.6 again, and the mixture was heated at 90 ° C. for 15 minutes to inactivate the enzyme. This was diluted with water to adjust the concentration of the decomposed product of the present invention to 4% (g / g) to prepare a sample. The viscosity of this sample was 132.3 cP at 4 ° C. and 60 rpm with a B-type viscometer (manufactured by Tokyo Keiki Co., Ltd., hereinafter used).

Comparative Example 1 To 1 L of water, 5 g of citric acid and 50 g of psyllium powder (trade name: Psyllium Seed Gum Food Made P-100; Shikibo) were added and mixed.
It became a viscous liquid of H3.0. This was sealed in a pouch bag for food retort, and heat-treated under pressure in a steam sterilizer at 110 ° C. or 120 ° C. for 20 minutes (steam chamber temperature). After heat treatment, cool and dilute it with water to reduce the concentration of the decomposed product to 4% (g
/ g) and adjusted as a sample. The viscosity of this sample is B
A 110 ° C sample cannot be measured by a viscometer at 4 ° C and 60 rpm, and a 120 ° C sample has 24.0%.
It was cP. The sample treated at 110 ℃ was measured with a B-type viscometer at 4 ℃ and 1.5 rpm.
Met.

Comparative Example 2 40 g of indigestible dextrin (trade name: pine fiber; Matsutani Kagaku) derived from potato starch, which is a water-soluble dietary fiber, was dissolved in 1 L of water to prepare a sample. The viscosity of this sample is 6 ° C at 6 ° C with a B-type viscometer
It was 7.2 cP under the condition of 0 rpm.

Comparative Example 3 0.2 g of citric acid in 1 L of water,
Adjust the pH to 3.6 by adding 1 g of ascorbic acid, and use Tric
Cellulase derived from genus hoderma (trade name: Cellulase T
5 g of "Amano"4; Amano Pharmaceutical Co., Ltd.) was added. 20 g of psyllium powder (trade name: Psyllium Seed Gum Food Made P-100; Shikibo) was added and mixed to this, and the mixture was mixed at 45 ° C. for 2 hours.
4-hour stirrer (DC STIRRER DCL-R; manufactured by Tokyo Rika Kikai)
The enzyme was allowed to act with stirring. 90 after the reaction
The enzyme was inactivated by heating at 15 ° C for 15 minutes. Although the concentration of this solution was 2% by weight, the viscosity was 100,000 cP or more, and it was considered that psyllium was hardly decomposed.

[Test Example 1] Using the samples of Example 1, Comparative Example 1 or Comparative Example 2 described above, the bowel movement improving effect was compared by the following method based on the rat fecal increase amount.

Five-week-old SPF / VAF rats (closed colony SD rats; Charles River Japan) were
It is housed in an SPF room with a temperature of 23.5 ± 2.0 ° C. and a humidity of 55 ± 10%, and water and feed (brand name: powdered oriental mixed feed; manufactured by Oriental Yeast Co., Ltd.) are freely ingested and acclimated and raised for 1 week, Each group was divided into 8 animals so that there was no difference in body weight.

Each solution of the samples of Example 1, Comparative Example 1 or Comparative Example 2 was freeze-dried and then crushed by a centrifugal crusher, or undecomposed psyllium (trade name: Psyllium Seed Gum Food Made P-100; Shikibo). ) Was added to a cellulose-free feed (powder-oriental modified feed; oriental yeast) at a concentration of 4% (g / g), and as a blank the above cellulose-free feed was not mixed with these. Was prepared as a test feed.

The test feed and water were freely ingested, and feces from 3 to 5 days after the start of ingestion were collected, the wet weight was measured, and their properties were also confirmed. Since there were some differences in feed intake depending on the feed, the amount of feces for each feed was divided by the amount of feed intake, and the amount of feces per unit of each test feed was calculated. The group fed the diet without the addition of dietary fiber was set as a blank,
The group fed the feed to which undegraded psyllium was added was used as a control, and the difference in the amount of feces (the amount of increased feces per ingested feed unit increased by undegraded psyllium) was set to 100%, and various test substances (Example 1, Comparative Example) were compared. The increase in the amount of feces in the feed to which Example 1 or Comparative Example 2) was added was shown as a relative value with respect to the increase in the group fed with psyllium.

As a result, the increased amount of feces by each feed is shown in Table 1.

[0037]

[Table 1]

As is clear from the above results, the product of the present invention (Example 1) has a fecal increase amount (= bowel movement improving effect) of about 50% as compared with undegraded psyllium, whereas The decomposed psyllium, even if it has a viscosity of 10,000 cP after 110 ° C. treatment, retains only 35% of the effect, and it can be seen that the enzyme partial decomposition product of the present invention has an excellent bowel movement improving effect. . It is also understood that the effect of the product of the present invention is higher than that of indigestible dietary fiber.

Further, a partial decomposition product with an acid (Comparative Example 1,
In 2), the feces had an offensive odor and was slightly blackened, which was not preferable in terms of properties.

Next, pectinase and hemicellulase were treated alone, or psyllium was treated by changing the mixing ratio of both enzymes, and the effects of improving the viscosity and the bowel movement were compared.

The enzyme activity of pectinase (trade name: pectinase G "Amano"; Amano Pharmaceutical Co., Ltd.) and hemicellulase (trade name: cellulosin HC100; Hankyu Kyoei Bussan) used in each example is psyllium (trade name: psyllium). Seed gum Foodmate P-100; Shikibo) was used as a substrate to determine the release rate of reducing sugars using the Somogyi-Nelson method (Somogyi,
M . : J. Biol. Chem. , 195, 19 (1952)) was calculated as follows. One unit (U) in this method is the amount of enzyme that liberates a reducing sugar corresponding to 1 μmol xylose per minute from psyllium. The enzyme activity is measured as follows.

Buffer: 20 mM acetate buffer (pH 4.
8) Substrate solution: 0.5% by weight psyllium solution (dispersed in buffer solution) Enzyme solution: 1 mg / ml (dissolve each enzyme in buffer solution) 400 μl of the above substrate solution was put in a test tube, and at 1 ° C at 45 ° C.
Warm for 0 minutes. Enzyme solution 10 that was heated at the same time
Add 0 μl and incubate at 45 ° C. for 10 minutes. The amount of reducing sugars before and after the reaction was measured by the Somogi-Nelson method (however, 57
Xylose prepared from absorbance at 0 nm (standard product)
The enzyme activity was calculated and the result showed that pectinase (trade name: pectinase G “Amano”; Amano Pharmaceutical Co., Ltd.) was 24.6 U / g, hemicellulase (trade name: cellulosin HC100; Hankyu Kyoei Bussan). 23.2 U / g, the activity was almost the same. Therefore, the enzyme titer used was about 100 U in Example 1 and about 120 U in Examples 2 to 7 below. That is, 1 g of psyllium
When calculated as the enzyme titer used per hit, Example 1
Is about 2 U and Examples 2 to 7 are about 1.5 U.

[Example 2] To 1 L of water, 0.2 g of citric acid was added to adjust the pH to 3.6, and 5 g of the pectinase derived from the genus Aspergillus (trade name: pectinase G "Amano"; Amano Pharmaceutical Co., Ltd.) was added. Was added. Psyllium powder (trade name: Psyllium Seed Gum Food Made P-10
80 g of Shikibo) was added and mixed, and the enzyme was allowed to act while stirring with a stirrer (DC STIRRER DCL-R; made by Tokyo Rikakikai) at 45 ° C. for 24 hours. After the reaction, the pH was adjusted to 3.6 again by adding 2.8 g of citric acid,
The enzyme was inactivated by heating at 15 ° C for 15 minutes. This was diluted with water to adjust the concentration of the decomposition product of the product of the present invention to 4% (g / g) and used as a sample. The viscosity of this sample is B-type viscometer at 4 ° C,
It was 126.5 cP under the condition of 60 rpm.

[Example 3] 5 g of pectinase of Example 2
4 g of pectinase (trade name: pectinase G "Amano"; Amano Pharmaceutical Co., Ltd.) and hemicellulase (trade name:
Cellulosin HC100; manufactured by Hankyu Kyoei Bussan Co., Ltd.) was similarly prepared, and the viscosity was 143.3 cP.

[Example 4] 5 g of the pectinase of Example 2
Instead, 2.5 g of pectinase (trade name: pectinase G "Amano"; Amano Pharmaceutical Co., Ltd.) and 2.5 g of hemicellulase (trade name: cellulosin HC100; Hankyu Kyoei Bussan) were used in the same manner. It was 157.5 cP.

[Example 5] 5 g of pectinase of Example 2
Instead, 1 g of pectinase (trade name: pectinase G "Amano"; Amano Pharmaceutical) and hemicellulase (trade name:
4 g of cellulosin HC100; Hankyu Kyoei Bussan) was similarly prepared, and as a result, the viscosity was 190.7 cP.

[Example 6] 5 g of pectinase of Example 2
Instead of hemicellulase (trade name: cellulosin HC10
0; Hankyu Kyoei Bussan) As a result of the same adjustment using 5 g,
The viscosity was 192.7 cP.

[Test Example 2] Using the same rat as in Test Example 1, the effect of improving the bowel movement of the partially decomposed psyllium obtained in Examples 2 to 6 was measured by the same method. As a result, the respective effects of improving bowel movements (fecal volume increase rate) were as shown in Table 2 below.

[0049]

[Table 2]

From the above results, it is understood that the amount of increased feces (effect of improving bowel movements) in this example is about 50% of the effect of undegraded psyllium. Further, it is understood that the use of pectinase can reduce the viscosity more than the use of hemicellulase, although there is no significant difference in the amount of increased feces. It was possible to reduce the viscosity by about 35% with pectinase alone and by about 18% with a mixture of hemicellulase and an equivalent amount of pectinase, as compared to the degradation using hemicellulase alone. Therefore, when considering commercialization, it is considered advantageous to use pectinase as the enzyme.

[Embodiment 7] In the above Embodiments 2 to 6, the liquid after the reaction is colored gray, which is not a very good impression in appearance. Therefore, in the method of Example 3, 1 g (0.1% (g / g)) of ascorbic acid was added to the enzyme reaction solution in addition to citric acid, hemicellulase, pectinase, and psyllium, and Example 3 was used. Samples were prepared similarly. As a result, the obtained solutions were light yellow and more transparent than those of Examples 2 to 6, and were preferable for making liquid beverages as they were.

When the viscosity of the partially decomposed product obtained in Example 7 was measured by the same method as in each Example, the viscosity was 83.
5 cP, a significant improvement over the viscosity of Example 3,
It was shown that the addition of ascorbic acid can further reduce the viscosity in addition to the effect of preventing coloration and reducing turbidity.

Also in Example 7, the same test as in Test Example 1 was carried out to test the effect of improving bowel movements (amount of increased feces), and as a result, the amount of increased feces was 53.1 ± 6.4%. It was confirmed that the compound had an activity equal to or higher than that of the sample to which ascorbic acid was not added.

Example 8 The partially decomposed psyllium product of the present invention obtained in Example 7 was dried, solidified, and dried by a freeze-drying method.
Pulverized. When this powder was thoroughly stirred in water to form a 4% by weight solution, the viscosity was measured by the same method as in each of the examples, the viscosity was 46.5 cP, and the viscosity could be further lowered.

[Production Example 1] Liquid beverage 4% of the powdered partial decomposition product of psyllium of the present invention of the present invention, citric acid 0.15%, ascorbic acid 0.05%, fructose 2.78%, stevia 0.006%, peach juice powder 0.17
%, Peach flavor powder 0.20% was added to the water (all weight%), and the mixture was stirred until it was homogeneous to give a liquid beverage containing the decomposed product of the present invention. The viscosity was 46.0 cP when measured under the same conditions as in each example. The drink 2
Although I drank 00 ml, there was no problem with the viscosity and it was deliciously drinkable as a soft drink.

[Production Example 2] Jelly confectionery The partially decomposed psyllium product of the present invention obtained in Example 4 was dried, solidified and powdered by a freeze-drying method. This is 5.75
g, sugar 12.0g, 5 times concentrated grape juice 8g, gelling agent
0.7 g, citric acid 0.1 g, ascorbic acid 0.05 g, perfume 0.
After adding 73.3 g of water to 1 g and stirring well, the mixture was put in a jelly cup, heated and cooled to give a jelly form. The jelly confection had no significant difference in texture from the usual jelly confection, and could be eaten deliciously.

[0057]

The partial decomposition product of psyllium of the present invention has a bowel movement-improving effect of about half that of undecomposed psyllium, but it is more effective than acid decomposition products of psyllium and other foods currently used in the market. It has an effect of improving bowel movement which is obviously superior to that of fiber. Moreover, since the viscosity when dissolved in water is much lower than that of undecomposed psyllium, it can be taken as a liquid beverage. Therefore, according to the product of the present invention, it can be easily taken in the form of a beverage containing a dietary fiber product derived from psyllium having a high bowel movement-improving effect, and such a beverage is a person who tends to lack dietary fiber or a constipated person. It is extremely useful.

When the product of the present invention is added to other foods, the high viscosity which has been a problem in undegraded psyllium can be overcome, so that the processing work is easy and the influence on the texture is small. In addition, it is possible to obtain a food product that has a high bowel movement improving effect.

Front page continued (72) Inventor Takayoshi Mori 4-1-1 Nishinakajima, Yodogawa-ku, Osaka City, Osaka Prefecture Nissin Foods Co., Ltd. (72) Inventor Kazuhiko Ueda 4-1-1, Nishinakajima, Yodogawa-ku, Osaka-shi, Osaka No. 1 within Nisshin Foods Co., Ltd. (56) Reference JP-A 4-210639 (JP, A) JP-A 64-20063 (JP, A) (58) Fields investigated (Int.Cl. ⁷ , DB name) ) A23L 1/308 A23L 1/03-1/06 C08B 37/00

Claims (10)

(57) [Claims] 1. A psyllium having a psyllium decomposition activity.
Pectinase, hemicellulase and combinations thereof
Interacting with an enzyme selected from the group consisting of
The partial decomposition product of psyllium obtained by the above, the viscosity of an aqueous solution containing 4% by weight of the partial decomposition product was measured by a B-type viscometer at 4 ° C., 60 rpm, and a pH of about 4. In the case of 30 to 200 cP, a partial decomposition product of psyllium having a bowel movement improving effect 2. The partially decomposed psyllium product according to claim 1 , wherein the pectinase is derived from a strain of the genus Aspergillus . 3. A bowel movement-improving agent containing the partially decomposed psyllium product according to claim 1 or 2 as an active ingredient. 4. A bowel movement-improving agent containing the partially decomposed psyllium product according to claim 1 or 2 as an active ingredient and further containing ascorbic acid. 5. An edible composition containing the partially decomposed psyllium product according to claim 1 or 2 . 6. The edible composition according to claim 5 , further comprising ascorbic acid. Wherein said edible composition is a liquid beverage according
Item 7. An edible composition containing the partially decomposed psyllium product according to Item 5 or 6 . 8. A method for producing a partially decomposed product of psyllium, which comprises dispersing psyllium in water and adding an enzyme selected from the group consisting of pectinase having heme decomposition activity, hemicellulase and a combination thereof. After reacting the enzyme with the enzyme and then inactivating the enzyme, the viscosity when the reaction solution was adjusted to 4% by weight was B
Type viscometer, 4 ° C, 60 rpm, a method for producing a partially decomposed psyllium product, including a step of partially decomposing psyllium so as to be 30 to 200 cP when measured at a pH of about 4 9. The method for producing a partially decomposed psyllium product according to claim 8 , wherein the pectinase is derived from a strain of the genus Aspergillus. 10. The manufacturing method according to claim until deactivate the enzyme, the ascorbic acid in the reaction system is added
8. The method for producing a partially decomposed psyllium product according to 8 or 9.