JP3501237B2 - Mineral absorption enhancer - Google Patents

Description

Description: BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a mineral absorption enhancer containing lactobionic acid, in which galactose and gluconic acid are bonded, as an active ingredient. 2. Description of the Related Art Calcium intake of Japanese people has been below nutritional requirements for many years, and calcium intake is required. However, the average dietary habit of the Japanese requires considerable effort to continue to consume menus containing sufficient calcium. In addition, minerals such as zinc, copper, and magnesium other than calcium tend to be deficient, and there is an increasing interest in foods having high mineral absorbency and substances that promote mineral absorption. Furthermore, the treatment and improvement of anemia due to hemorrhage and iron deficiency, iron citrate, iron pyrophosphate, iron chloride, etc. iron and vitamin B ₁₂ preparation, such as iron sulfate are used, these iron is the body Has to be administered in relatively large amounts due to the very low absorption at However, in the case of oral administration of these preparations, when administered in large amounts, iron preparations have the problem of causing ironosis of the digestive tract wall and various side effects (such as nausea, nausea, and constipation). On the other hand, as a known substance, a disaccharide lactobionic acid represented by a general formula of O-β-D-galactopyranosyl- (1-4) -D-gluconic acid is known.
It is not known that lactobionic acid promotes calcium absorption. The inventors of the present invention have conducted intensive studies for a substance that promotes the absorption of calcium as described above, and as a result, the general formula is O-β-D-galactopyra. The inventors have found that lactobionic acid represented by nosyl- (1-4) -D-gluconic acid promotes calcium absorption, and have completed the present invention. Therefore, an object of the present invention is to provide a mineral absorption promoter containing lactobionic acid as an active ingredient. [0005] In the present invention, the general formula is O
Lactobionic acid represented by -β-D-galactopyranosyl- (1-4) -D-gluconic acid is used as an active ingredient of a mineral absorption promoter. This lactobionic acid can be obtained by using lactose as a substrate and allowing microorganisms having lactose dehydrogenase activity such as Pseudomonas graveorens to act thereon, or can be obtained by oxidizing lactose with bromine or the like. [0006] Lactobionic acid having the effect of promoting mineral absorption is orally administered, for example, as tablets, granules, liquids or capsules such as sugar-coated tablets and tablets in order to exert its effect in vivo in humans and livestock. It can also be used as a mineral-enhancing food material by adding it to drinks, soups, cheeses, jellies, breads, noodles, sausages, etc., or confectionery such as gums and candies. Further, it can be used as a feed additive. The amount of lactobionic acid is not particularly limited, but is 1 mg / kg body weight / day or more, and preferably 1 to
1,000 mg / kg body weight / day is appropriate. [0007] The mineral absorption promoter of the present invention containing lactobionic acid as an active ingredient promotes the absorption of minerals necessary for living organisms such as calcium, iron, magnesium, zinc, and copper. It is also expected to have an effect of improving symptoms such as osteoporosis and iron deficiency anemia. Next, the present invention will be specifically described with reference to examples and test examples. Example 1 In accordance with a conventional method, a fruit juice beverage having a mineral absorption promoting effect was produced according to the mixing ratio shown in Table 1. [Table 1] (Unit:% by weight) ──────────────────────────────────── Mixing Isomerized sugar 15.0 fruit juice 10.0 citric acid 1.0 lactobionic acid (manufactured by Wako Pure Chemical Industries) 0.1 flavor 0.1 water 73.8 ─────────────────────────── Example 2 According to a conventional method, a jelly having a mineral absorption promoting effect was produced according to the compounding ratio shown in Table 2. [Table 2] (Unit:% by weight) ──────────────────────────────────── Fruit juice 20.0 Granulated sugar 15.0 Candy starch 5.0 Agar 1.0 Lactobionic acid (manufactured by Wako Pure Chemical Industries) 0.5 Fragrance 0.1 Water 58.4 ──────────────────────────── Example 3 A calcium absorption promoter containing the lactobionic acid of the present invention was prepared. After 2,200 g of calcium, 1,232 g of corn starch, 100 g of crystalline cellulose, 68 g of carboxymethylcellulose calcium and 400 g of lactobionic acid (manufactured by Wako Pure Chemical Industries, Ltd.) were mixed in a kneader, and kneaded while spraying 500 ml of water dropwise. Next, the kneaded material was granulated with a single-screw oscillator equipped with a 20-mesh screen, and dried with a fluidized-bed dryer. The dried product was pulverized with a flash mill and sized to obtain a tableting powder.
The sucrose fatty acid ester 8
After mixing 0 g with a V-type mixer, the mixture was tableted with a tablet machine equipped with a punch having a diameter of 11 mm to obtain a tablet having an average weight of 0.35 g. Example 4 According to a conventional method, a dog breeding feed (dog food) having a mineral absorption promoting effect was produced according to the mixing ratio shown in Table 3. [Table 3] (Unit:% by weight) ──────────────────────────────────── Large Bean meal 13.7 Skim milk powder 14.0 Soybean oil 4.0 Corn oil 2.0 Palm oil 2.0 Corn starch 23.0 Flour 15.0 Bran 8.0 Vitamin mixture 2.0 Mineral mixture 9.0 Cellulose 2.3 Lactobionic acid (manufactured by Wako Pure Chemical Industries) 0.5 0.5 ───────────────────────── The composition of the vitamin mixture is shown in Table 4, and the composition of the mineral mixture is shown in Table 5. [Table 4] ──────────────────────────────────── Vitamin A 1,500 IU Vitamin D ₃ 300 IU Vitamin E 6.8 mg Vitamin B ₁ 0.9 mg Vitamin B ₂ 0.4 mg Vitamin B ₆ 0.5 mg Vitamin B ₁₂ 3.4 mg Vitamin C 50.0 mg Pantothenic acid 4.0 mg Folic acid 0.2 mg Choline 200.0 mg Biotin 24.4 μg Inositol 50.0 mg Niacin 10.5 mg ─ ─────────────────────────────────── Sucrose was added to make the total amount 2 g. [Table 5] CaCO ₃ 3.0 g KH ₂ PO _{4 2.0 g NaH 2 PO 4 1.5} g MgO 0.5 g MnCO 3 40.0 mg FeC 6 H 5 O 7 30.0 mg 70% ZnO 10.0 mg 55% CaCO 3 4.5 mg KlO 3 0.65 mg Na 2 SeO 3 · 5H 2 O 0.05 mg CrK (SO ₄ ) ・ 12H ₂ O 5.0 mg 加 え Add sucrose The total amount was 9 g. Test Example 1 Animal experiments were conducted on the effect of lactobionic acid on promoting mineral absorption. Animal experiments were performed using 6-week-old SD male rats in groups of 6 animals. As shown in Table 6, the experimental diet used was a mixture of 5% by weight of each saccharide. [Table 6] (Unit: g / 100g) ──────────────────────────────────── Group A Group B Group C──────────────────────────────────── casein 20.0 20.0 20.0 Corn starch 65.2 60.2 60.2 Cellulose 5.0 5.0 5.0 Corn oil 5.0 5.0 5.0 Vitamin mix 1.0 1.0 1.0 Methionine 3.5 3.5 3.5 DL-methionine 0.3 0.3 0.3 Lactose-5.0-Lactobionic acid--5.0 ─────────────────群 Group A: control group, Group B: lactose administration group, Group C: lactobionic acid administration group Table 7 shows the component values of each experimental meal. And Table 8 below. [Table 7] (Unit: g / 100g) Group A Group B Group C──────────────────────────────────── Carbohydrate 63.19 64.19 64.09 Protein 17.45 17.39 17.31 Fat 5.45 5.58 5.45 Moisture 10.82 9.81 10.10 Ash 3.09 3.03 3.05 Ａ A Group: control group, Group B: lactose administration group, Group C: lactobionic acid administration group [Table 8] (Unit: mg / 100g)群 Group A Group B Group C───────────────────────── ─────────── Sodium 109 108 97 Potassium 350 350 350 Calcium 490 490 495 Magnesium 4 1 42 42 Iron 3.8 3.9 4.0 Zinc 3.6 3.7 3.6 Copper 0.55 0.55 0.56 Manganese 4.7 5.0 5.0 Phosphorus 600 610 610 ───────────────────────────群 Group A: control group, Group B: lactose-administered group, Group C: lactobionic acid-administered group.
One week after the administration of the experimental diet, feces and urine were collected, excreted calcium and magnesium were measured, and calculated by the following equation. Mineral absorption rate (%) = [(amount of ingested mineral−amount of excreted mineral) / (amount of ingested mineral) × 100 The results are shown in FIG. According to this, it was found that the lactobionic acid administration group promoted the absorption of minerals such as calcium and magnesium as compared with the lactose administration group known as a calcium absorbing substance. Test Example 2 21-day-old Wistar rats (body weight 45-
To 50 g), an iron-free diet (manufactured by Oriental Yeast Co., Ltd.) with an iron content of 0.25 mg / 100 g feed was given for 3 weeks to produce anemic rats having a blood hemoglobin value of 7 g / 100 ml or less. And 1
The group consisted of 5 animals, and the following test feed was orally administered at a rate of 1 ml / day for 6 weeks while continuing to receive the iron-free diet. Test group 1: ferrous iron-only diet Test group 2: 0.2 mg / ml of ferrous sulfate as iron Test group 3: 0.2 mg / ml of ferrous sulfate as iron + 200 mg / ml of lactobionic acid Test group 4: sulfate Six weeks after the administration of the test feed, 0.2 mg / ml lactose using iron as iron, blood was collected from the tail vein six weeks after administration of the test feed, and the hemoglobin value was measured using an automatic blood cell counter (manufactured by Toa Medical Electronics Co., Ltd.). Table 9 shows the results. ９ Hemoglobin value (mean ± standard) Deviation) ──────────────────────────────────── Test group 1 4.9 ± 0.3 (g / ml) test Group 2 10.5 ± 0.8 Test group 3 16.1 ± 1.0 Test group 4 11.6 ± 0.9 ───────────────────────────────── Thus, it was found that the lactobionic acid-administered group exhibited a better anemia treatment effect than the inorganic iron-ferrous sulfate alone-administered group and the lactose-administered group. Since the lactobionic acid of the present invention has an action of promoting the absorption of minerals, the medicine, food and drink and feed containing the lactobionic acid are useful for supplementing minerals.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 shows the experimental results of the mineral absorption promoting effect of Test Example 1.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification code FI A61P 19/10 A61P 19/10 // C07H 15/04 C07H 15/04 F (56) References JP-A-5-316997 (JP) , A) The American Journal of Clinical Nutrition, 1982, Vol. 36, No. 6, p. 1162-1169 (58) Field surveyed (Int.Cl. ⁷ , DB name) A61K 31/7032 A23L 1 / 29,1 / 30 A23K 1/16 303 C07H 3/00-3/10 C07H 7/00-7 / 06 C07H 15/04 REGISTRY (STN) CA (STN) MEDLINE (STN) EMBASE (STN) JICST file (JOIS)

Claims (1)

(57) [Claims] (1) A mineral absorption promoter comprising lactobionic acid as an active ingredient.