JP3526806B2 - Cosmetics - Google Patents

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a cosmetic composition containing a salt obtained from seawater to prevent skin aging such as wrinkles and spots, or to promote cell growth.

[0002]

2. Description of the Related Art Human skin gradually ages with aging, and it is known that various external stimuli other than aging cause skin aging. In particular, ultraviolet rays in sunlight cause cross-linking or cutting of collagen, elastin, etc., which are components of the extracellular matrix, and thereby reduce the elasticity and moisturizing properties of the skin, resulting in wrinkles, dry skin, and rough skin. Cause of. Skin that has undergone such changes is more susceptible to external stimuli, cell activity is reduced, and the regenerative capacity of the extracellular matrix is reduced. The loss of skin gloss, the increase in stains, dullness, etc., which progresses in a short period of time, is often due to the strong external stimulus as described above. Therefore, in order to prevent skin aging due to external irritation, ultraviolet absorbers and moisturizers have been incorporated into cosmetics.

In addition, cosmetics are blended with ingredients that inhibit collagenase and elastase to try to maintain the extracellular matrix in a normal state, and vitamin C and vitamin E.
It has also been carried out to incorporate a component having a cell growth promoting action such as.

However, it is difficult to completely block harmful ultraviolet rays with an ultraviolet absorber that can be usually mixed,
If added in a large amount, irritation to the skin becomes a problem. It is also difficult to sufficiently activate skin cells with a moisturizer, a collagenase inhibitor, etc., and a cell growth promoting component such as vitamin C has problems in safety, stability, etc., and it was not always satisfactory.

[0005]

The object of the present invention is to contain a salt obtained from seawater, prevent skin aging such as wrinkles and spots, and promote cell proliferation, thereby reducing irritation.
It is to provide cosmetics with high safety.

[0006]

Means for Solving the Problems As a result of intensive studies to solve the above problems, the present inventors have completed a cosmetic composition according to the present invention having an antiaging effect and / or a cell growth promoting effect. Came to let.

[0007]

According to the first aspect of the present invention, after salt water is acidified, a basic solution is added to remove precipitates formed, the resulting solution is concentrated, cooled and filtered to obtain a salt ( It relates to a skin cosmetic containing at least one of B) and a salt (C) obtained by evaporating a solvent from the filtered solution.

[0009]

According to a second aspect of the present invention, the salt obtained by acidifying seawater, adding a basic solution to remove the formed precipitate, concentrating the obtained solution, cooling and filtering the salt ( The present invention relates to a skin cosmetic containing an aqueous solution in which at least one of B) and a salt (C) obtained by evaporating a solvent from a filtered solution is dissolved in water.

[0011]

A third aspect of the present invention relates to a skin cosmetic , wherein the total amount of both the salt (B) and the salt (C) is 0.1 to 2.0% by weight.

A fourth aspect of the present invention relates to a cosmetic for skin, which has at least one of an antiaging effect and a cell growth promoting effect.

The cosmetic of the present invention can be in the form of any cosmetic applied to the skin, such as lotion, emulsion, cream, pack, gel cosmetic, powder cosmetic, soap and liquid detergent. .

Further, the cosmetics in the present invention include hair growth agents, bath agents, medicated cosmetics, medicated cosmetics, that is, medicated soaps, medicated shampoos, among quasi drugs prescribed by the Pharmaceutical Affairs Law.
Medicated rinse, medicated lotion, medicated cream / milky lotion, medicated pack, hair dye, cosmetics for hair, general cream / milky lotion, shaving cream or lotion, suntan / sunblock lotion, cosmetic oil, ointment, suntan / sunblock oil, It can be applied to white powder, powder, foundation, perfume, pack, nail cream, enamel, enamel remover, eyebrow, blusher, eye cream, eye shadow, mascara, eyeliner, lipstick, lip balm, toothpaste and bath cosmetics.

The cosmetic of the present invention comprises the constituents usually used in the production of the cosmetic, such as oil, surfactant,
Thickener, moisturizer, preservative, fragrance, colorant, chelating agent,
It may contain one or more amino acids and sugars.

Further, the cosmetic composition of the present invention contains, if necessary, any amount of glycyrrhizinic acid, or a salt thereof, or a derivative thereof (stearyl glycyrrhetinate, etc.), allantoin, azulene, baicalin, horse chestnut extract, and auburn. Extract, Coptis extract, Ascorbic acid, Magnesium ascorbyl phosphate, Ellagic acid,
Arbutin, kojic acid, placenta extract, mulberry acid, kujin extract, waremout extract, age extract, vitamins, tocopherol and its derivatives, natural vitamin E, collagen, elastin hyaluronic acid, chondroitin sulfate It may contain one or more components.

[0018]

A fifth aspect of the present invention is a method for producing salt (B) or salt (C) which is a component of a skin cosmetic composition, which comprises adding a first step of acidifying seawater and a basic solution. A second step of removing the produced precipitate and the solution obtained in the second step are concentrated, cooled, and then filtered to form the salt (B), or comprising a third step of generating the salts obtained (C) by evaporation of the solvent from the filtered solution the the cosmetic skin
Providing fee manufacturing method of containing component is a salt (B) or salt (C) of.

[0020]

[0021] A sixth aspect of the present invention is a method for producing a skin cosmetic , comprising a first step of acidifying seawater, a second step of adding a basic solution and removing a precipitate formed, and The solution obtained in the second step is concentrated and cooled, and then filtered to produce a salt (B), or a salt obtained by evaporating a solvent from the filtered solution ( third process and, at least one method for producing a skin cosmetic comprising a fourth step, the to one of the contained in the skin cosmetic of the salt (B) and the salts (C) to produce a C) I will provide a.

[0022]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in more detail with reference to the following examples, but the following examples do not limit the present invention.

To 500 liters of seawater, 10 liters of an aqueous solution prepared by dissolving calcium phosphate in which 5% concentrated sulfuric acid was added to remove precipitates was added, and the mixture was allowed to stand for 3 hours and then insoluble matters were removed by filtration. This makes seawater p
It became H1.6. Next, 15 kg of sodium hydroxide was added to 500 liters of seawater having a lowered pH, and the mixture was left for 10 hours. At this time, the formed precipitate was filtered off, and 10 liters of the remaining seawater was heated to remove water, thereby obtaining a 1.5 liter concentrated solution. The concentrated solution is rapidly cooled to form a precipitate, and the salt (B) obtained by filtering is removed;
A total of 200 g of the salt (C) obtained by drying the solution after separation by filtration was obtained. A stock solution was obtained by dissolving 100 g of a mixture of the salt (B) and the salt (C) (hereinafter referred to as "cell growth promoting substance") in water.

[0024]

[Examples 1 to 3],

[Comparative Examples 1 and 2] Using the following (a) to (c) as raw materials, cosmetics were produced by a conventional method. (A) (1) 4% by weight of dipropylene glycol (2) 1,3-butylene glycol 2 (3) polyoxyethylene (60) hydrogenated castor oil 1 (4) ethyl alcohol 4 (5) methyl paraoxybenzoate 1 (6) Citric acid 0.2 (7) Sodium citrate 0.1 (8) Perfume 0.02 (b) See Table 1 (c) Purified water to 100 in total.

[0025]

[Table 1]

[0026]

[Test Example 1] The lotions of Examples 1 to 3 and Comparative Examples 1 and 2 were tested for cell growth promoting action by the following MTT method.

MTT method (J.immunol.Method 93,157.198
6): Precultured normal human neonatal dermal fibroblasts (N
B1RGB) was dispensed into a 96-well microplate, RITC80-7 medium (Kyoto Pharmaceutical Co., Ltd., pH 7.2) containing 10% FBS culture solution in which the sample was dissolved was added, and the mixture was mixed at 37 ° C. under 5% CO ₂ for 3 days.
Incubate for a day. Then, MTT reagent (3- (4,5-dimethyl-2
-thiazolyl) -2,5-diphenyl-2H tetrazolium bromide, 5m
20 μl of g / ml) is added and incubated for 4.5 hours (active mitochondria-derived active enzyme in the grown cells reacts with the MTT reagent, and the color of the reagent which was yellow is 57
It turns blue-yellow with an absorption peak at 0 mm). Then, the absorbance (A) at 570 mm is measured using a microplate reader. Separately, the same culture is performed without adding the sample, and the absorbance (B) is measured. Further, the absorbance (C) when the sample is added and the same treatment is performed without adding the cells is measured. Finally, the absorbance (D) when the same treatment is performed without adding the sample and the cells is also measured.

Each absorbance measurement value was 650 measured at the same time.
The absorbance in mm is subtracted to correct for the effects of increased turbidity due to grown cells. From each corrected absorbance, the cell growth promotion rate is calculated by the following formula. Cell growth promotion rate (%) = [{(AC)-(BD)}
/ (BD)] × 100 The results are shown in Table 2, and the cell growth-promoting action was confirmed for the lotions of all Examples, and particularly the cell growth of the lotions of Examples 1 to 3 was extremely excellent. A promoting effect was confirmed. However, the lotions of Comparative Examples 1 and 2 did not show a cell growth promoting action.

[0029]

[Table 2] Cell growth promotion rate (%) (Note: “Sample concentration” is the sample concentration in Medium 1.)

[0030]

Example 4 A cream was produced by treating the following raw materials by a conventional method. (1) Cell growth promoting substance 2.0 (2) Acetostearyl alcohol 3.5 (3) Squalane 30.0 (4) Beeswax 3.0 (5) Reduced lanolin 5.0 (6) Stearyl paraoxybenzoate 0. 1 (7) Polyoxyethylene (20) sorbitan monostearate 2.0 (8) Glycerin monostearate 5.0 (9) 1,3-butylene glycol 5.0 (10) Perfume 0.05 (11) Make up to 100 with purified water.

[0031]

Comparative Example 3 A cream was produced by removing the cell growth promoting substance from the raw material of Example 4.

[0032]

[Test Example 2] A woman who complains of skin dullness, wrinkles, and dryness 1
The following use tests were carried out on the creams of Example 4 and Comparative Example 3 with 8 persons (ages 33 to 45 years old: persons having a score of 1 to 2 for both items according to the following criteria) as panelists.

Test method: The panelists were divided into 2 groups of 9 persons, one of which was the cream of Example 4 and the other was the cream of Comparative Example 3 for 50 days each twice a day in the morning and evening.
It was applied to the face. After 50 days, the number and depth of gloss, wrinkles and dryness, and wrinkles on the coated surface were observed with the naked eye, and answers were given according to the following criteria. [Skin gloss and dullness] Rating Observation result 1 No skin gloss and strong dullness. Many exfoliations of the stratum corneum are observed. 2 There is no gloss on the skin, there is dullness on the whole, and peeling of the stratum corneum can be seen in places. 3 There is slight gloss on the skin and no dullness is visible. The stratum corneum is not peeled off with the naked eye, but it is peeled off when lightly rubbed with a cotton swab. 4 The skin is shiny and firm. Almost no peeling of the stratum corneum even with rubbing with makeup cotton [Wrinkles] Rating Observation result 1 There are many wrinkles around the corners of the eyes, and many are seen. Wrinkles around the 2nd corner are seen, and the number is slightly large. 3 Wrinkles around the outer corner of the eye are observed, but the number is small. 4 Wrinkles around the corners of the eyes are not recognized under normal facial expression. The test results are shown in Tables 3 and 4.

[0034]

[Table 3] Effect of improving skin gloss, dullness and dryness

[0035]

[Table 4] Wrinkle improvement effect

[0036]

Example 5 An emulsion having the following composition was produced by a conventional method. (1) Cell growth promoting substance 2.0 (2) Carrot extract 0.065 (3) Cetyl alcohol 0.5 (4) Squalane 3.0 (5) Beeswax 1.0 (6) Vaseline 2.0 (7) Polyoxyethylene (20) sorbitan monooleate 1.0 (8) Glycerin monostearate 1.0 (9) Dipropylene glycol 4.0 (10) Ethanol 2.0 (11) Methyl roseoxybenzoate 0.12 (12) Sodium hyaluronate 0.1 (13) The total amount is 100 with purified water.

[0037]

Comparative Example 4 An emulsion was prepared by removing the cell growth promoting substance from the raw material of Example 5.

[0038]

[Test Example 3] The same test as in Test Example 2 was performed. The test results are shown in Tables 5 and 6.

[0039]

[Table 5] Improvement effect on gloss, dullness and dryness of skin

[0040]

[Table 6] Wrinkle improvement effect

[0041]

Example 6 A pack having the following composition was produced by a conventional method. (1) Cell growth promoting substance 1.0 (2) Polyvinyl alcohol 15.0 (3) Polyethylene glycol 3.0 (4) Propylene glycol 7.0 (5) Ethanol 10.0 (6) Methylparaben 0.05 (7) Perfume 0.05 (8) Make up to 100 with purified water.

[0042]

Comparative Example 5 A pack was produced by removing the cell growth promoting substance from the raw material of Example 6.

[0043]

[Test Example 4] The same test as in Test Example 2 was performed. The test results are shown in Tables 7 and 8.

[0044]

[Table 7] Improvement effect on gloss, dullness and dryness of skin

[0045]

[Table 8] Wrinkle improvement effect

[0046]

Example 7 An ointment having the following composition was produced by a conventional method. (1) Cell growth promoting substance 0.5 (2) Cetyl alcohol 18.0 (3) Mokurou 20.0 (4) Polyoxyethylene (20) monooleate 0.25 (5) Glycerin monostearate 0.25 (6) Vaseline 40.0 (7) The total amount is 100 with purified water.

[0047]

Comparative Example 6 An ointment was produced by removing the cell growth promoting substance from the raw material of Example 7.

[0048]

[Test Example 5] The same test as in Test Example 2 was performed. The test results are shown in Tables 9 and 10.

[0049]

[Table 9] Improvement effect on gloss, dullness and dryness of skin

[0050]

[Table 10] Wrinkle improvement effect

From the above test results 3 to 5, Examples 5 to 7 were obtained.
In all of the example products, the skin improving effects such as gloss, stains, wrinkles, and dullness were observed as compared with Comparative example products 4 to 6 containing no cell growth promoting substance.

[0052]

INDUSTRIAL APPLICABILITY According to the present invention, there is provided a cosmetic composition which has cell growth promoting action and anti-aging action and is effective in improving skin such as spots, wrinkles and dullness.

Continuation of the front page (56) Reference JP-A-11-12154 (JP, A) JP-A-10-182412 (JP, A) JP-A-10-182347 (JP, A) JP-A-8-217631 (JP , A) JP 8-104607 (JP, A) JP 2000-72656 (JP, A) JP 7-17850 (JP, A) JP 6-256161 (JP, A) JP 10 -194732 (JP, A) JP-A-8-91829 (JP, A) (58) Fields investigated (Int.Cl. ⁷ , DB name) A61K ^7/ 00-7/50

Claims (6)

(57) [Claims] 1. A salt (B) obtained by acidifying seawater, adding a basic solution to remove the formed precipitate, concentrating the obtained solution, cooling and filtering. ,
For skin containing at least one of salt (C) obtained by evaporating the solvent from the filtered solution
Cosmetics . 2. A salt (B) obtained by acidifying seawater, adding a basic solution to remove the formed precipitate, concentrating the resulting solution, cooling and filtering. ,
A skin cosmetic containing an aqueous solution in which at least one of salt (C) obtained by evaporating a solvent from the filtered solution is dissolved in water. 3. The skin cosmetic according to claim 1, wherein the total amount of both the salt (B) and the salt (C) is 0.1 to 2.0% by weight. Fee . 4. The method of claim 1, characterized in that it comprises at least one of anti-aging effects and cell growth promoting action
4. The skin cosmetics according to any one of 3 to 3 . 5. A salt (B) which is a component contained in a skin cosmetic.
Or a method for producing salt (C), which comprises a first step of acidifying seawater, a second step of adding a basic solution and removing a precipitate formed, and a step of obtaining the salt in the second step. The resulting solution is concentrated and cooled, and then the salt (B) is obtained by filtering, or the salt (C) is obtained by evaporating the solvent from the filtered solution. And a third step of: (3) A method for producing a salt (B) or a salt (C), which is a component contained in a skin cosmetic . 6. A method for producing a skin cosmetic , comprising a first step of acidifying seawater, a second step of adding a basic solution and removing a precipitate formed, and the second step of The solution obtained in the process is concentrated and cooled, and then filtered to form a salt (B), or the solvent is evaporated from the filtered solution to obtain a salt (C). third process and, at least one method for producing a skin cosmetic comprising a fourth step, the to one of the contained in the skin cosmetic of the salt (B) and the salts (C) to be generated.