JP3605528B2 - Weight gain inhibitor - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a weight gain inhibitor comprising as an active ingredient royal jelly and / or a degradation product of royal jelly by protease.
[0002]
[Prior art]
In recent years, it is said to be the time of satiety, and the eating habits have been enriched, and the number of people who suffer from obesity due to lack of daily exercise is increasing. Along with this, so-called adult diseases such as hypertension and diabetes have become a major problem. Therefore, for health or beauty reasons, an increasing number of people take a diet composition to lose weight.
[0003]
For example, water-insoluble dietary fibers such as gymnema extract, gardenia extract, wheat bran, corn hulls, and water-soluble dietary fibers such as guar gum and indigestible dextrin are known as having a diet effect (anti-obesity effect). Yes. Since these components have an action of inhibiting the absorption of nutrients into the living body, they exhibit an excellent diet effect.
[0004]
By the way, royal jelly is a milky white and sour acid substance secreted from the secretory glands of the bees (hypopharyngeal gland, large vagina gland), and is a substance serving as a special bait for growing the queen bee. The ingredients of royal jelly are still unclear, but contain abundant nutrients such as vitamins, minerals, amino acids, acetylcholine, 10-hydroxydecenoic acid, sterols, hormones, and prevent menopause, anti-anemic, and anti-aging , Anti-radiation action, anti-cancer action, blood flow increase action, anti-arteriosclerosis action (cholesterol lowering action, blood pressure normalizing action), rheumatism / neuralgia prevention action, health promotion action, etc. It has been known. For this reason, it has long been used for health foods such as raw types, capsule types, and beverage types, pharmaceuticals, and cosmetics.
[0005]
Collected stock solution, so-called raw royal jelly, has a unique taste and odor and many people feel resistance when eating. Recently, alcohol extracted royal jelly, protease treated royal jelly, etc. have been developed and added In many cases, it is consumed as a drink. For example, Japanese Patent No. 2623044 (Japanese Patent Laid-Open No. 5-123119) by the present applicant contains components similar to raw royal jelly by treating raw royal jelly with two or more types of proteases having different sites of action on the substrate. Moreover, it is disclosed that a transparent royal jelly degradation product that is transparent and excellent in stability, can be easily added to beverages and the like, and is thus easily taken orally.
[0006]
Regarding the physiological activity of enzymatically digested royal jelly, a hair-recovery agent for oral consumption containing a peptide having a molecular weight of 3,000 or less as an active ingredient by the degradation product of royal jelly by the applicant of the present invention (Japanese Patent Laid-Open No. 8-104645), An infection defense function enhancer (JP-A-8-59499) containing a peptide having a molecular weight of 3,000 or less as an active ingredient is disclosed.
[0007]
[Problems to be solved by the invention]
However, taking too much a component having a diet effect as described above may cause loose stool or diarrhea, inflammation of the intestinal mucosa, a decrease in immunity, or mental instability, In addition, it has been difficult to add a sufficient amount (effective amount) to foods and the like due to problems of taste and texture.
[0008]
Therefore, an object of the present invention is to provide a weight gain inhibitor that can be expected to have various other physiologically active functions as well as a weight gain inhibitory effect, is safe, easy to take orally, and can be taken easily. is there.
[0009]
[Means for Solving the Problems]
In the course of studying the bioactive function of the enzymatic degradation product of royal jelly, the present inventors have found that royal jelly and the enzymatic degradation product of royal jelly surprisingly have an effect of suppressing weight gain. It came to be completed.
[0010]
That is, the present invention is a weight gain inhibitor characterized by comprising royal jelly and / or a degradation product of royal jelly with a protease as an active ingredient.
[0011]
According to the present invention, royal jelly and / or a degradation product of royal jelly with a protease provides an effect of suppressing weight gain as shown in Examples described later, and a diet effect can be expected without causing diarrhea symptoms. . In particular, the royal jelly degradation product has no taste and odor, is easily dissolved in water, and does not coagulate or precipitate protein due to heat or pH, so it is safe, easy to ingest, and can be easily ingested.
[0012]
Furthermore, various physiologically active functions possessed by royal jelly, and physiologically active functions such as a hair-growth effect, a defense function enhancing effect, and a calcium absorption promotion effect (Japanese Patent Application No. 10-90808) possessed by the degradation product of royal jelly protease. I can expect.
[0013]
In the present invention, it is preferable that the degradation product contains a peptide having a molecular weight of 3,000 or less as a main component.
[0014]
DETAILED DESCRIPTION OF THE INVENTION
The active ingredient of the weight gain inhibitor of the present invention is royal jelly and / or a degradation product of royal jelly by protease.
[0015]
In the present invention, raw royal jelly is used as the royal jelly. Further, in the present invention, a degradation product of a royal jelly protease (hereinafter abbreviated as a royal jelly degradation product) is combined with a protease, preferably two or more types of proteases having different action sites for a substrate, in a royal jelly suspension. It can be obtained by sequentially adding and maintaining the temperature at room temperature or higher to cause an enzyme reaction.
[0016]
As the protease, those commonly used in food processing, such as acidic proteases derived from microorganisms and plants, neutral proteases, alkaline proteases, and digestive enzymes derived from mammals such as pepsin and pancreatin may be used. Among these, it is preferable to select and use two or more types having different action sites for the substrate.
[0017]
The treatment with protease is performed while measuring the degradation rate of protein over time, and the final degradation rate varies depending on the protease used, but it is preferably performed until the degradation rate is 75% or more, more preferably 85% or more. Good. The details of the production method are described in the above-mentioned Japanese Patent No. 2623044.
[0018]
The thus obtained royal jelly degradation product contains a peptide having a molecular weight of 3,000 or less, and contains the same components as raw royal jelly except that the protein is decomposed and converted into a peptide (in the solid content, Protein: 36-42%, carbohydrate: 45-52%, lipid: 13-15%, decenoic acid: 5-6%).
[0019]
And this royal jelly decomposition product does not have the irritation | stimulation which stabs a throat even if it eats as it is, but rather it has some taste and sweetness. In addition, it is transparent and excellent in stability when it is made into an aqueous solution, and it can be easily added to beverages and is easily taken orally.
[0020]
The royal jelly decomposition product may be used in the form of a solution as it is, or may be pulverized by means such as freeze-drying. In addition, as such a royal jelly decomposition product, for example, a commercially available product such as “Bio-san royal” (trade name, manufactured by Biox Co., Ltd.) can be used.
[0021]
In the weight gain inhibitor of the present invention, the royal jelly and / or royal jelly degradation product may be, for example, a capsule or the like, or may be a tablet by adding an appropriate excipient. Also, it can be added to and dissolved in honey, nutrition drinks, soft drinks, etc. to make fluids such as beverages. When added to beverages, etc., the above royal jelly and / or royal jelly degradation product is added in an amount of 0.5 to 5% by weight, preferably 1 to 3% by weight in terms of dry matter.
[0022]
The preferred intake of royal jelly and / or royal jelly degradation product, which is an active ingredient of the weight gain inhibitor of the present invention, is 0.5 to 10 g per day in terms of dry matter.
[0023]
【Example】
Hereinafter, the present invention will be specifically described with reference to examples.
Manufacturing Example Warm water was added to 100 g of raw royal jelly to prepare 1 kg of a royal jelly suspension, and the pH was adjusted to 4 using a 20% sodium hydroxide solution. To this suspension, 0.1 g of mammalian digestive enzyme pepsin (1: 10,000, manufactured by Sigma) was added and subjected to enzyme treatment at 45 ° C. for 6 hours to obtain a pepsin-treated solution. Next, after adjusting this pepsin-treated solution to pH 8 using a 20% sodium hydroxide solution, 0.1 g of pancreatin (2,000,000 units / g, manufactured by Sigma) which is a digestive enzyme of mammals is added. Then, the enzyme treatment was performed at 45 ° C. for 6 hours.
[0024]
This enzyme-treated solution was heated at 80 ° C. for 10 minutes to deactivate the enzyme, and then filtered to remove foreign substances and insoluble residues to obtain a transparent solution. This solution was concentrated under reduced pressure to 100 g, which was the same weight as the raw royal jelly used first, to obtain a transparent solution-like royal jelly decomposition product. In addition, this royal jelly decomposition product is the same as that marketed as “Bio-san royal” (trade name, manufactured by Biox Co., Ltd.).
[0025]
The enzyme treatment time was measured at 0, 2, 4, and 6 hours after the start of the enzyme reaction, and the protein degradation rate was measured by the following method, and the time when the degradation rate reached the maximum.
[0026]
・ Measurement method of proteolysis rate To 2.0 ml of sample, add an equal amount of 10% trichloroacetic acid (TCA), perform centrifugation, dilute the supernatant, colorimetrically determine by the Raleigh method, The change in quantity was examined. The crude protein in royal jelly was obtained by colorimetric determination in the same manner using a solution to which an equal amount of water was added instead of the TCA solution. And the protein degradation rate was calculated | required by the formula shown to following Numerical formula 1.
[0027]
[Expression 1]
Decomposition rate (%) = {(c−b) / (a−b)} × 100
a: Crude protein content of royal jelly degradation product b: Content of TCA soluble component of royal jelly degradation product c: Content of TCA soluble component of enzyme-treated sample
The protein degradation rate after 6 hours enzyme (pancreatin) treatment determined by the above method was 91.6%.
[0029]
Moreover, molecular weight distribution was measured and compared with the crude protein contained in the obtained royal jelly degradation product and raw royal jelly. The result is shown in FIG. In FIG. 1, A is the royal jelly decomposition product obtained in the above production example, and B is the result of raw royal jelly.
[0030]
From the result of FIG. 1, when the molecular weight distribution of the crude protein in the transparent royal jelly degradation product obtained in the above production example is compared with the molecular weight distribution of the crude protein in the raw royal jelly, in the royal jelly degradation product obtained in the production example Most of them have a molecular weight of 10,000 or more, and a peptide having a molecular weight of 500 to 3,000 is the main component. This was also confirmed from the results of electrophoresis and the like.
[0031]
That is, the royal jelly degradation product obtained in the above production example contains the same components as raw royal jelly except that the protein is decomposed and converted into a peptide, and the peptide is said to have a physiological function in particular. It turns out that many peptides with a molecular weight of hundreds to thousands are contained.
[0032]
In addition, the royal jelly degradation product obtained in the above production example has no taste and odor, and even when added to a weakly acidic beverage, it was easily dissolved without aggregation and precipitation like raw royal jelly.
[0033]
Example 10 Using a KKA ^y strain male mouse (spontaneous diabetes model mouse) aged 10 weeks, a physiological saline administration group (hereinafter referred to as a control group), a raw royal jelly administration group (hereinafter referred to as a raw RJ group), and a royal jelly degradation It is divided into food administration groups (hereinafter referred to as PRJ group) (6 animals in each group), and under the environment of light / dark cycle every 12 hours, solid feed CA-1 (trade name, manufactured by Clea Japan Co., Ltd.) and water are They were raised for 4 weeks as free intake.
[0034]
In the control group, the dose of physiological saline per administration is 500 mg / animal, and in the raw RJ group and PRJ group, the doses of raw royal jelly and royal jelly degradation product are determined for each decenoic acid amount. The raw royal jelly is 300 mg / kg body weight (105 mg / kg body weight in terms of dry matter), and the royal jelly degradation product (BX-7 L-50, trade name, manufactured by Biox) is 730 mg / kg body weight. (121 mg / kg body weight in terms of dry matter). Each test substance was stored frozen by subdividing the required amount, and thawed under refrigeration, and then administered continuously for 28 days using a gastric sonde. In addition, food intake and water intake were measured every 7 days, body weight was measured before administration of each test substance, and weight gain was observed. The results of food intake and water intake are shown in FIG. 2, and the results of weight gain are shown in FIG.
[0035]
As can be seen from FIG. 2, there was no significant difference in food intake / water intake in each group. On the other hand, from FIG. 3, it was found that the weight gain of the raw RJ group and the PRJ group was significantly suppressed compared to the control group (p <0.05).
[0036]
【The invention's effect】
As described above, the weight gain inhibitor of the present invention contains a royal jelly and / or a royal jelly degradation product, so that it can bring about a weight gain inhibitory effect and has a diet effect without causing diarrhea symptoms. I can expect. In particular, the royal jelly degradation product has no taste and odor, is easily dissolved in water, and does not coagulate or precipitate protein due to heat or pH, so it is safe, easy to ingest, and can be easily ingested.
[0037]
Further, various physiologically active functions possessed by the royal jelly and / or the royal jelly degradation product can be expected.
[Brief description of the drawings]
BRIEF DESCRIPTION OF DRAWINGS FIG. 1 is a diagram showing the molecular weight distribution of a crude protein in a royal jelly degradation product obtained in a production example and a crude protein in raw royal jelly.
FIG. 2 is a diagram showing food intake and water intake of each group during the experiment period.
FIG. 3 is a diagram showing the weight gain of each group during the experimental period.

Claims (2)

A weight gain inhibitor characterized by comprising royal jelly and / or a degradation product of royal jelly by protease. The weight gain inhibitor according to claim 1, wherein the degradation product contains a peptide having a molecular weight of 3,000 or less as a main component.

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