JP3607559B2 - Disinfectant composition - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a disinfectant composition and a disinfecting method.
[0002]
[Prior art and problems to be solved by the invention]
Conventionally, chlorine-based disinfectants such as sodium hypochlorite, calcium hypochlorite, and sodium dichloroisocyanurate have been widely used as disinfectants in a wide range of environments. Among them, hypochlorite such as sodium hypochlorite is widely used in terms of price and effect, but for sterilization and sterilization of microorganisms required in various fields such as medical and food industries, Furthermore, many proposals have been made to improve its effectiveness. For example, JP-A-57-61099 discloses a liquid sterilizing bleaching composition containing hypochlorite, an alkaline substance, and a specific quaternary ammonium salt type cationic surfactant in specific weight ratios. JP-A-7-233396 discloses a bactericidal cleaning agent for medical devices such as an artificial dialysis machine containing hypochlorite, an anionic surfactant, an alkali agent and a chelating agent.
[0003]
However, although conventional hypochlorite fungicides have some effects on general bacteria and mold (mycelium), they are easy for viruses with higher chemical resistance, spores formed by gonococci, and mold spores. A sufficient operation cannot be expected.
[0004]
Japanese Patent Application Laid-Open No. 11-148098 discloses a solid sterilizing detergent using an alkaline earth metal hypochlorite such as highly bleached powder (calcium hypochlorite). In addition, alkaline earth metals such as calcium cause scales and scum, resulting in a decrease in sterilization efficiency.
[0005]
Furthermore, JP-A-7-328638 describes that an adhesive for reducing surface tension is added to electrolytically oxidized water to increase the adhesion to the outer surface of an object to be sterilized, which is excellent in terms of sterilization effect. Chlorine gas is generated and there is a safety problem.
[0006]
In JP-A-59-93799, amine oxide is added to a liquid detergent containing hypochlorite, and in JP-A-59-98200, amine oxide is added as follows. Although they are disclosed for use as thickeners for bleach containing chlorite, there is no mention of sterilization, particularly sterilization of highly resistant spores and viruses.
[0007]
It is an object of the present invention to obtain a bactericidal composition having a high bactericidal effect and excellent safety and workability by a simple treatment.
[0008]
[Means for Solving the Problems]
The present invention relates to a liquid fungicide composition comprising an aqueous solution having a pH (25 ° C.) of 3 to 8 containing hypochlorous acid (A) and a pH adjuster (B). Moreover, this invention relates to the said disinfectant composition which contains 1 or more types of surfactant (C) further.
[0009]
In addition, the present invention relates to a sterilization method containing hypochlorous acid (A) and a pH adjuster (B), and bringing an aqueous solution having a pH (25 ° C.) of 3 to 8 and an effective chlorine concentration of 5 to 5000 ppm into contact with microorganisms. .
[0010]
DETAILED DESCRIPTION OF THE INVENTION
An aqueous solution containing hypochlorous acid (A) can be obtained by a known method for producing electrolytic oxidized water, for example, by electrolyzing an electrolyte-containing aqueous solution by a diaphragm system or by acidifying hypochlorite. The concentration of hypochlorous acid (A) in the fungicidal composition of the present invention is preferably 5 ppm to 5% by weight, more preferably 5 ppm to 1% by weight, especially 5 ppm to 0.5% by weight as the effective chlorine concentration. In the present invention, the effective chlorine concentration is measured by JIS K-0101 “Iodine Method”.
[0011]
The pH (25 ° C.) of the fungicide composition of the present invention is 3 to 8, preferably 5 to 8, and more preferably 5 to 7. This pH is adjusted by a pH adjuster (B). This pH adjuster can maintain the composition of the present invention at a pH (25 ° C.) of 3 to 8 by adding it, and can be selected from compounds used as alkali agents and buffers. . Moreover, a pH adjuster (B) may have functions other than such a pH adjustment function.
[0012]
Examples of the pH adjuster include alkali metal hydroxides, alkaline earth metal hydroxides, inorganic acids or salts thereof, organic acids or salts thereof, and the like. Specifically, sodium hydroxide, potassium hydroxide, calcium hydroxide, hydrochloric acid, sodium sulfate, sodium nitrate, sodium chloride, sodium carbonate, potassium bicarbonate, sodium bicarbonate, potassium bicarbonate, magnesium sulfate, magnesium nitrate, chloride Magnesium, magnesium carbonate, trisodium phosphate, tripotassium phosphate, disodium hydrogen phosphate, dipotassium hydrogen phosphate, sodium dihydrogen phosphate, potassium dihydrogen phosphate, sodium polyphosphate, citric acid, sodium citrate, Examples include potassium citrate, potassium dihydrogen citrate, potassium hydrogen phthalate, and succinic acid. Among them, sodium hydroxide, potassium hydroxide, hydrochloric acid, sodium sulfate, magnesium sulfate, sodium carbonate, potassium carbonate, sodium bicarbonate, potassium bicarbonate, trisodium phosphate, tripotassium phosphate, disodium hydrogen phosphate, phosphorus One or more selected from dipotassium oxyhydrogen, sodium dihydrogen phosphate, potassium dihydrogen phosphate and sodium polyphosphate are preferred.
[0013]
The fungicidal composition of the present invention preferably contains one or more surfactants (C). The surfactant can be selected from an anionic surfactant, a cationic surfactant, a nonionic surfactant, and an amphoteric surfactant, and those that are stable in a hypochlorous acid aqueous solution are used. Of these, cationic surfactants and amphoteric surfactants are preferable, and amine oxides are particularly preferable. As the amine oxide, alkyldimethylamine oxide is preferable, and those having an alkyl group having 8 to 18 carbon atoms are particularly preferable. The ratio of the surfactant (C) to the hypochlorous acid (A) in the composition is (A) (as effective chlorine concentration) / (C) = 10/1 to 1/10 (weight ratio), further 5 / 1-1 / 5, especially 5 / 1-1 / 2 is preferred.
[0014]
The disinfectant composition of the present invention may further contain an organic acid or a salt (D) thereof from the viewpoint of improving the disinfecting performance. These may function as pH adjusters. Examples of organic acids or salts thereof (D) include saturated dibasic acids such as malonic acid, succinic acid, glutaric acid, adipic acid, and sebacic acid, or salts thereof, unsaturated dibasic acids such as fumaric acid, maleic acid, and the like. Examples include salts. A saturated dibasic acid or a salt thereof is preferable, a saturated dibasic acid having 3 to 10 carbon atoms or a salt thereof is preferable, and a succinic acid or a salt thereof is particularly preferable.
[0015]
The disinfectant composition of the present invention may contain a thickener, a fragrance, a colorant and the like in addition to the components (A) and (B).
[0016]
In use, the fungicidal composition of the present invention is used as a further diluted aqueous solution. This diluted aqueous solution has a component (A) having an effective chlorine concentration of 5-5000 ppm, more preferably 5-1000 ppm, especially It is preferable to contain 5-500 ppm.
[0017]
The sterilization method of the present invention comprises the above components (A) and (B), and further a component (C) at a pH (25 ° C.) of 3 to 8, preferably 5 to 8, more preferably 5 to 7, and effective chlorine. It is carried out by bringing an aqueous solution having a concentration of 5 to 5000 ppm, preferably 5 to 1000 ppm, particularly 5 to 500 ppm into contact with the microorganism. The microorganism means general bacteria, filamentous fungi, viruses, mold spores, bacterial spores and the like.
[0018]
Although the contact method of aqueous solution is not limited, Methods, such as spreading | spraying, spraying, immersion, filling, etc., are mentioned, You may wipe off a target object with what impregnated aqueous solution in the suitable support | carrier. Although the contact time of the aqueous solution is not limited, depending on the microorganism, a sufficient effect can be obtained even with a short contact time of 30 seconds or less, particularly 10 seconds or less. Moreover, although the temperature of the aqueous solution made to contact is also not limited, 20-60 degreeC is preferable.
[0019]
The bactericidal composition and the bactericidal method using the same of the present invention have a wide bactericidal spectrum and have a high effect on not only bacteria (fungi) but also viruses and spores, and thus are useful as a bactericidal method in a wide range of fields. For example, it is used for sterilization of hospitals, nursing homes, food processing factories, cleaning facilities, kitchen walls, floors, windows, etc., or equipment, fixtures, and products (for example, beverages) containers used in them.
[0020]
【The invention's effect】
According to the present invention, a bactericidal composition and a bactericidal method having a high bactericidal effect and excellent safety and workability can be obtained.
[0021]
【Example】
In the so-called electrolytically oxidized water obtained by the diaphragm system, hypochlorous acid water (pH (25 ° C.) 2.7, effective chlorine concentration 50 ppm) generated on the anode side was used, and the disinfectant composition was as follows. A product was prepared. The hypochlorous acid concentration is an effective chlorine concentration measured by JIS K-0101 “iodine method”.
(1) Adjust to pH 5 (Example 1) or pH 11 (Comparative Example 1) with a 0.1 mol / L sodium hydroxide aqueous solution to obtain a bactericide composition.
(2) The pH is adjusted to 6 with a 0.1 mol / L sodium hydroxide aqueous solution, and lauryldimethylamine oxide is added so as to have a concentration of 25 ppm to obtain a fungicide composition (Example 2).
(3) Adjust to pH 7 with 0.1 mol / L potassium dihydrogen phosphate-disodium hydrogen phosphate buffer to obtain a bactericidal composition (Example 3).
[0022]
The following tests were conducted using each fungicide composition. The results are shown in Table 1. In addition, the generation of chlorine gas in each fungicide composition was at a low level to the extent that there was no problem with safety.
[0023]
[1] Spore kill test Bacillus subtilis ATCC6633, a spore bacterium, was cultured on an SCD agar medium (manufactured by Nippon Pharmaceutical Co., Ltd.) for 50 days at 30 ° C., and the resulting bacterium was washed twice by centrifugation. Then, heat treatment was performed at 65 ° C. for 30 minutes to obtain a bacterial spore suspension (10 ⁵ cells / ml).
[0024]
50 μl of this bacterial spore suspension was inoculated into 2 ml of a bactericide composition, allowed to act at room temperature for 5 minutes, and then 50 μl of this bacterial contact solution was collected and used for post-culture SCDLP medium (containing 3.3% sodium thiosulfate) A micro Petri dish (CORNING, 96-Cell Wells) containing 0.2 ml was inoculated. After culturing at 30 ° C. for 48 hours, the growth of the bacteria was observed with the naked eye, and it was observed whether the bacteria were growing on a micro petri dish. The case where there was no growth of the fungus was designated as “◎”, and the case where it was present was designated as “x”.
[0025]
[2] Fungicidal test mold (fungus, Aspergillus niger IFO6341) was cultured in PDA medium for 30 days at 25 ° C., and the resulting cells were homogenized using the glass ball method, and then foreign substances were removed with sterile gauze Suspended bacterial solution (10 ⁵ cells / ml) was obtained.
[0026]
After inoculating 50 ml of this suspension bacterial solution into 2 ml of the bactericidal composition and allowing it to act at room temperature for 5 minutes, 0.1 ml of this bacterial contact solution was collected and PDA medium for postculture (containing 3.3% sodium thiosulfate) ). After culturing at 25 ° C. for 7 hours, mold growth was observed with the naked eye and evaluated in the same manner as described above.
[0027]
[Table 1]

Claims (2)

A liquid bactericidal composition comprising an aqueous solution of pH 5 to 8 containing hypochlorous acid (A), a pH adjuster (B), and an amine oxide (C) . A sterilization method containing an aqueous solution containing hypochlorous acid (A), a pH adjuster (B), and an amine oxide (C) and having a pH (25 ° C) of 5 to 8 and an effective chlorine concentration of 5 to 5000 ppm.