Deprecated: The each() function is deprecated. This message will be suppressed on further calls in /home/zhenxiangba/zhenxiangba.com/public_html/phproxy-improved-master/index.php on line 456
JP3661044B2 - Barley-derived gastrointestinal function improvement and cholesterol elevation inhibitor - Google Patents
[go: Go Back, main page]

JP3661044B2 - Barley-derived gastrointestinal function improvement and cholesterol elevation inhibitor - Google Patents

Barley-derived gastrointestinal function improvement and cholesterol elevation inhibitor Download PDF

Info

Publication number
JP3661044B2
JP3661044B2 JP32438496A JP32438496A JP3661044B2 JP 3661044 B2 JP3661044 B2 JP 3661044B2 JP 32438496 A JP32438496 A JP 32438496A JP 32438496 A JP32438496 A JP 32438496A JP 3661044 B2 JP3661044 B2 JP 3661044B2
Authority
JP
Japan
Prior art keywords
barley
group
cholesterol elevation
cholesterol
gastrointestinal function
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP32438496A
Other languages
Japanese (ja)
Other versions
JPH10165120A (en
Inventor
浩司 玉川
誠 福島
好司 遠藤
Original Assignee
株式会社はくばく
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 株式会社はくばく filed Critical 株式会社はくばく
Priority to JP32438496A priority Critical patent/JP3661044B2/en
Publication of JPH10165120A publication Critical patent/JPH10165120A/en
Application granted granted Critical
Publication of JP3661044B2 publication Critical patent/JP3661044B2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Landscapes

  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Cereal-Derived Products (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)

Description

【0001】
【発明の属する技術分野】
この発明は大麦糠から得られた大麦由来の消化管機能改善、コレステロール上昇抑制剤、および同剤を含む健康食品に関するものである。
【0002】
【従来の技術】
食物繊維(dietary fiber)は一般に体内で利用されず、むしろ栄養素の利用効率を阻害するものとしてじゃま物扱いされ、これまでの栄養学では無視されてきた。玄米を精白して白米を食べるように、なるべく繊維を取り除いて食べるよう食品の精製が工夫されてきた。しかし、1970年代から種々の疾病の予防に食物繊維が効果を発揮するということが各国で証明され、その摂取の重要性が認識されるところとなった。
【0003】
近年、穀類、豆類等の外皮から得られた食物繊維が肥満予防、便通改善、さらに種々の整理活性効果を有することが認識され、健康食品として注目されている。特に小麦ふすまは、製粉工程において胚乳と胚芽が取り分けられた後に残る表皮の部分で、主成分をセルロース、ヘミセルロース、リグニンとした不溶性食物繊維である。その便秘予防効果は古くから知られるところである。最近では小麦ふすまを主原料としたシリアル食品が特定保健用食品として認可され、「整腸作用」の効能が許可されている。また糖尿病、心臓血管疾患、大腸ガン、腸憩室症などに対する防御的効果に強い関心が寄せられている。例えば特開昭58−41824号には小麦ふすままたは小麦ふすまから澱粉質、蛋白質、脂質、無機質等を除去した残部からアルカリで抽出されてなるヘミセルロースを主成分として含有する血清コレステロール上昇抑制物質が提案されている。
【0004】
また、特開昭62−79756号には、小麦ふすまを水洗および脱脂してなる低油脂含有小麦ふすまが提案されている。
【0005】
さらに特開平1−265868号には、小麦ふすまを粉砕および気体分級処理することにより得られるダイエタリー繊維製品が提案されている。
【0006】
【発明が解決しようとする課題】
しかしながら、上記特開昭58−41824号の発明はヘミセルロースの含有率を高めるためにアルカリ抽出、除たんぱくなどの複雑な操作を必要とする。このため製造コストが高くつき、飲食品への添加剤として手軽に用いることができなかった。
【0007】
また、アルカリや除蛋白剤などの人体に危険を及ぼすおそれのある処理剤や薬剤を用いるため、これらが製品中に残留しないように細心の注意を払う必要があった。
【0008】
さらに、特開昭62−79756号および特開平1−265868号の発明も、前者には水処理による排水設備と脱脂する際のアルコールとその回収設備が、また後者においても溶剤処理は行なわないものの、乾燥機、シフター、分級ロータ等を使用しており、決して単純な処理操作とはいえない。
【0009】
そこでこの発明は小麦ふすまと同様に食物繊維が豊富に含有されている大麦糠を原料として、より簡単な工程で、危険な処理剤や薬剤を用いることなく製造することが可能な大麦由来の消化管機能改善、コレステロール上昇抑制剤、および同剤を含む健康食品を提供しようとするものである。
【0010】
すなわち、本発明者らは上記目的を達成するため鋭意研究した結果、大麦を搗精する際に発生する歩留り80〜100%の糠区分のうち、特に60M篩(目開き0.25mm)の通過区分が、市販の小麦ふすま素材と比較し、より優れた消化管機能改善作用およびコレステロール上昇抑制作用を有することを見いだし、本発明を完成するに至ったのである。
【0011】
【課題を解決するための手段】
すなわちこの発明の大麦由来の消化管機能改善およびコレステロール上昇抑制剤は、食用大麦を製造する際に発生した糠を含有することを特徴とするものである。
【0012】
この発明の大麦由来の消化管機能改善およびコレステロール上昇抑制剤は、上記大麦糠が搗精歩留り80〜100%の区分であることをも特徴としている。
【0013】
この発明の大麦由来の消化管機能改善およびコレステロール上昇抑制剤は、上記食物繊維含量が40%以上であり、かつ総食物繊維量に占めるヘミセルロースの含有率が60%以上としていることをも特徴としている。
【0014】
この発明の大麦由来の消化管機能改善およびコレステロール上昇抑制剤は、上記大麦糠をふるい等で粒度分けして、その粒度を60M篩(目開き0.25mm)通過の区分としてなることをも特徴としている。
【0015】
この発明の大麦由来の消化管機能改善およびコレステロール上昇抑制剤は、使用される大麦糠が粗脂肪を約5%以上含有する組成からなることをも特徴としている。
【0016】
本発明の原料とされる搗精歩留まり80〜100%の大麦糠は、大麦粒の外側部分を意味し、通常は大麦の精麦工程において食用麦の残余のものからなっている。これらの大麦糠の組成は、大麦の種類により多少異なるが、例えばカナダ産大麦(品質B1602)の場合、水分10.1%、灰分6.1%、粗蛋白12.1%、粗脂肪5.3%、食物繊維56.6%となっている。しかし摂取する時期、および品種については特に限定的ではない。
【0017】
本発明では、まずこの大麦糠を60Mの篩にかけ、総食物繊維量に占めるヘミセルロース含有率の少ない60Mオーバー区分を除去し、ヘミセルロース含有率の高い60M篩通過区分(総食物繊維量が40%以上であり、総食物繊維量に占めるヘミセルロース含有率が60%以上)を得るのみである。必要によってさらに粉砕して粒度を調整することにより、本発明の大麦由来の消化管機能改善およびコレステロール上昇抑制剤を得ることができる。なお、本発明でいう食物繊維量とは、Southgate法によって測定される難消化性多糖類を意味している。
【0018】
また本発明では、この物質を各種の食品に添加することによって消化管機能改善剤およびコレステロール上昇抑制剤を含有する健康食品を得ることができる。このような飲食品としては、例えばパン、煎餅、ケーキ、ビスケット、クッキー等のパン・菓子類や、そば、うどん、中華麺などの麺類や、天ぷら・フライなどの総菜類、おだんご類などの各種のものが上げられる。
【0019】
この場合、食品中への上記物質の添加量は、消化管機能改善効果およびコレステロール上昇抑制効果を十分に付与するため、13%(重量)以上とすることが好ましい。
【0020】
なお本発明の物質は、消化管機能改善効果およびコレステロール上昇抑制効果を有する健康食品としてそのまま利用することもできる。
【0021】
本発明では篩により分けるという非常に簡単な工程で消化管機能改善効果およびコレステロール上昇抑制効果を有する物質を得ることができる。またアルカリ、除蛋白剤などを用いる必要がないので、人体に対して有害な処理剤や薬剤が残留するおそれもない。
【0022】
本発明においては、大麦を搗精した際の歩留り80〜100%の大麦糠区分のうち、60M篩の通過区分が重要であり、市販小麦ふすまと比較し、明らかに消化管機能改善効果およびコレステロール上昇抑制効果が認められる。
【0023】
しかし、60M篩オーバーの区分には、市販小麦ふすまと比較し、有意な差が認められない。その理由として、消化管機能改善効果に関しては、60M篩の通過区分中に含まれるヘミセルロースの保水性が非常に高いこと、脂溶性の機能性成分を含む脂質含量が低いこと、また腸内細菌に利用されやすいこと等が考えられる。
【0024】
【発明の実施の形態】
以下、本発明の実施の形態を実施例に基づいて詳細に説明する。
【0025】
【実施例】
1995年にカナダで収穫された六条大麦(品種B1602)をRM−5C研削式搗精機とRM−3C研削式搗精機(いずれも株式会社佐竹製作所製)による連座搗精により歩留り80%まで精麦した。大麦糠成分は、マイクロシフター(ダルトン社製)により30M篩(目開き0.5mm)オーバー区分と60M篩(目開き0.25mm)通過区分に分画した。前者をBarley bran30(以下BB−30と略す)、後者をBarley bran60(以下BB−60と略す)とした。また、以下の試験例において比較した小麦ふすま(以下WBと略す)は市販のものを用いた。以上の試料を粒度の違いによる生理効果への影響を除くため、ポリトロン(ホソカワミクロン社製)にて、すべて100M篩(目開き0.15mm)を全量通過するよう粉砕した。各々の試料の成分分析結果を表1に示す。
【0026】
【表1】

Figure 0003661044
<試験例>実施例1で得られた試料の消化管機能改善効果およびコレステロール上昇抑制効果を調べるため、ラットを用いた動物実験を行なった。
(a)実験飼料実験に用いた飼料組成を表2に示す。実験群は、無繊維(Fiber−free、以下FFと略す)群とセルロースパウダー(以下CPと略す、東洋濾紙株式会社製)5w/w%配合群を対照として、BB−30群、BB−60群、WB群をそれぞれ食物繊維レベルで5w/w%含む実験飼料とした。なお、BB−30、BB−60、WBの各群は、一般成分値より飼料中の蛋白質、脂質、澱粉のレベルが等しくなるように調整した。
【0027】
【表2】
Figure 0003661044
(b)動物飼育方法4週齢のSprague−Dawlay系ラット(初体重70−85g、東京実験動物株式会社製)を用い、AIN−76組成に基づく基礎試料で7日間予備飼育後、体重にしたがい1群7匹とする5群に分け、実験に供した。ラットはステンレス製個別ゲージに入れ、四域室内は23±1℃、湿度50±10%、12時間明暗サイクル(明期8:00〜20:00)に調節した。表2に示した各飼料群および水(水道水)は自由摂取させ、28日間本飼育を行なった。
(c)分析および統計処理方法消化管通過時間(以下GTTと略す)の測定は、本飼育開始第8日目と15日目の2回行なった。すなわち、8:00〜20:00まで絶食し、マーカーとしてカルミンを0.5(w/w)%含む飼料を投与した。30分ごとに糞中の色素の有無を確認し、マーカーが初めて確認されるまでに要した時間を測定することにより行なった(結果は2回の平均値)。
【0028】
糞量の測定は、本飼育終了前の5日間毎日採取して行なった。糞量測定後、真空凍結乾燥処理し、乾燥糞量を測定した。
【0029】
各飼料群の消化管の特性に及ぼす影響を調べるため、飼育期間終了後に3時間絶食させ、ネンブタール麻酔下にて開腹した。盲腸については迅速に取り出して重量を測定後、内容物の一部は超純水とともにホモジナイズし、ガラス電極にてpHを測定した。残りの内容物は液体窒素にて凍結後、−80℃で保存しておき、短鎖脂肪酸をDeschnerらの方法によりガスクロマトグラフ(GC−12A、株式会社島津製作所製)にて定量した。
【0030】
各飼料群の肝臓コレステロール値に及ぼす影響を調べるため、開腹後、肝臓を氷冷した生理的食塩水にて洗い、クロロホルム:メタノール混液(2:1)で行なうFolchの方法にしたがい脂質を抽出し、総コレステロールはZakらの方法、トリグリセリドはDannoらの方法、リン脂質はBartlett法により測定した。これら分析値の平均値の差の検定には、SASコンピューターパッケージを用い、数値は一元配置分散分析(one−way ANOBA)の後、有意差の検定はDuncan’s multiple range testにより行なった。その結果を表3〜表6に示す。
【0031】
【表3】
Figure 0003661044
【0032】
【表4】
Figure 0003661044
【0033】
【表5】
Figure 0003661044
【0034】
【表6】
Figure 0003661044
(d)結果に対する説明1)消化管機能改善に関する説明糞量、糞の水分量および内容物の腸内通過時間、盲腸の特性に及ぼす各試験群の影響を表3および表4に、また盲腸内容物中の短鎖脂肪酸に及ぼす影響を表5に示した。最も特徴的な違いが見られたのが盲腸内容物のpHで、FF群と比較し、CP群およびBB−30群はほとんど変わらなかったのに対し、BB−60群は有意に盲腸内容物pHが低下した。またその程度は、WB群との比較においても有意であった。盲腸内容物重量についても、WB群、BB−30群と比較し、有意差はないものの、最も多かったのがBB−60群であった(表4)。これらの原因の1つは、BB−60と他の試験群の成分特性の違いが発酵産物(例えば短鎖脂肪酸等のpHを低下させる物質)の生成量に影響を及ぼしているからである(表5)。つまり、BB−60群は、他の試験群と比較し、腸内細菌に分解・発酵されやすい物質を多く含んでおり、同時に腸内細菌の増植を促している可能性が十分にある。
【0035】
さらに、糞量、糞の水分含有率のバランスを考えると、BB−60群は他の試験群と比較し、最も糞の水分量が多かった。このことは、BB−60群が保水性の高いものであることを示している。また、GTTについても、BB−60群はFF群、CP群と比較し、有意に短縮されていることはもちろん、BB−30群とも有意差を示し、有意差はないものの、WB群と比較しても短縮されていた(表3)。GTTに及ぼす食餌成分の影響は複雑であるが、一般的に保水性の高い食物繊維は、糞便量を増加させるのでGTTを短縮させるといわれている。したがって、BB−60群が他の試験群と比較し、最もGTTを短縮させた要因として、BB−60群自体の保水性が十分慣用している可能性がある。また、結腸内にSCFAを直接投与した実験では、収縮する強さ(ある程度GTTと相関性があるといわれている)は、酪酸が最も効果的であることが認められている。このことと今回の結果(表5)を踏まえると、BB−60群中の食物繊維の代謝産物である短鎖脂肪酸組成特性が、明らかにGTTの短縮に寄与していることがわかる。
【0036】
以上の結果より、BB−60群は他の試験群と比較し、明らかに消化管機能改善作用が優れており、その作用は、WB群と比較して同等、あるいはそれ以上であることがわかった。
【0037】
2)コレステロール上昇抑制に関する説明各試験群が、肝臓コレステロールに及ぼす影響を表6に示した。トリグリセリド量において、BB−60試験群は他の試験群と比較し、有意に低かった。また総コレステロール量においても他の試験群と比較し、低かった。短鎖脂肪酸生成量とコレステロール量は比較的負の相関性があるといわれている。このことから、BB−60群がWBと比較し、コレステロール上昇抑制に優れている理由として短鎖脂肪酸の生成量が多いためであることが考えられた(表5)。
【0038】
以上の結果より、BB−60群はWB群と比較し、より優れたコレステロール上昇抑制作用があることがわかった。
【0039】
【発明の効果】
この発明によれば、小麦ふすまと同様に食物繊維を豊富に含有している大麦の糠を原料として、より簡単な工程で、危険な処理剤や薬剤を用いることなく製造することが可能な消化管機能改善およびコレステロール上昇抑制剤、および同剤を含有する健康食品を非常に安価に提供することができるようになった。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an improvement of gastrointestinal function derived from barley obtained from barley koji, a cholesterol elevation inhibitor, and a health food containing the same.
[0002]
[Prior art]
Dietary fiber is generally not utilized in the body, but rather treated as an obstacle to the utilization efficiency of nutrients, and has been ignored in the past nutritional science. The refinement of food has been devised to remove as much fiber as possible and to eat white rice by polishing white rice. However, since the 1970s, it has been proven in various countries that dietary fiber is effective in preventing various diseases, and the importance of its intake has been recognized.
[0003]
In recent years, it has been recognized that dietary fibers obtained from husks such as cereals and beans have anti-obesity, improved bowel movement, and various organizing activity effects, and are attracting attention as health foods. In particular, wheat bran is an insoluble dietary fiber whose main component is cellulose, hemicellulose, and lignin, which is the part of the epidermis that remains after endosperm and germ are separated in the milling process. Its anti-constipation effect has long been known. Recently, cereal foods made mainly from wheat bran have been approved as foods for specified health use, and the effect of “intestinal regulation” is permitted. There is also a strong interest in protective effects against diabetes, cardiovascular disease, colon cancer, intestinal diverticulosis and the like. For example, Japanese Patent Laid-Open No. 58-41824 proposes a serum cholesterol elevation inhibitory substance containing hemicellulose as a main component, which is extracted from wheat bran or the remainder obtained by removing starch, proteins, lipids, minerals, etc. from wheat bran. Has been.
[0004]
Japanese Patent Application Laid-Open No. 62-79756 proposes a low-fat-containing wheat bran obtained by washing and degreasing wheat bran.
[0005]
Further, JP-A-1-265868 proposes a dietary fiber product obtained by pulverizing and gas classifying wheat bran.
[0006]
[Problems to be solved by the invention]
However, the above-mentioned invention of JP-A-58-41824 requires complicated operations such as alkali extraction and deproteinization in order to increase the content of hemicellulose. For this reason, the manufacturing cost was high, and it could not be easily used as an additive to food and drink.
[0007]
In addition, since treatment agents and chemicals that may cause danger to the human body such as alkalis and deproteinizing agents are used, it is necessary to pay close attention to prevent them from remaining in the product.
[0008]
Furthermore, in the inventions of JP-A-62-79756 and JP-A-1-265868, the former includes drainage equipment by water treatment, alcohol for degreasing and its recovery equipment, and the latter does not perform solvent treatment. , Dryers, shifters, classifying rotors, etc. are used, and it is by no means a simple processing operation.
[0009]
Therefore, the present invention uses barley meal containing abundant dietary fiber in the same manner as wheat bran as a raw material, and it is a digestion derived from barley that can be produced in a simpler process without using dangerous treatment agents or chemicals. An object of the present invention is to provide a vascular function improvement agent, a cholesterol elevation inhibitor, and a health food containing the agent.
[0010]
That is, as a result of intensive studies to achieve the above-mentioned object, the present inventors have determined that the passing section of a 60M sieve (mesh size 0.25 mm) among the 80% to 100% yield of the straw that occurs when barley is refined. However, as compared with a commercially available wheat bran material, it has been found that it has a better gastrointestinal function improving action and a cholesterol elevation-inhibiting action, and the present invention has been completed.
[0011]
[Means for Solving the Problems]
That is, the barley-derived gastrointestinal tract function improvement and cholesterol elevation inhibitor of the present invention is characterized by containing cocoons generated when producing edible barley.
[0012]
The barley-derived gastrointestinal function improvement and cholesterol elevation inhibitor of the present invention is characterized in that the barley koji is classified into 80% to 100% yield.
[0013]
The barley-derived gastrointestinal function improvement and cholesterol elevation inhibitor of the present invention is characterized in that the dietary fiber content is 40% or more and the content of hemicellulose in the total dietary fiber content is 60% or more. Yes.
[0014]
The barley-derived gastrointestinal tract function improvement and cholesterol elevation inhibitor of the present invention is characterized in that the barley koji is classified into particles by sieving, and the particle size is classified as passing through a 60M sieve (mesh 0.25 mm). It is said.
[0015]
The barley-derived gastrointestinal function improvement and cholesterol elevation inhibitor of the present invention is also characterized in that the barley meal used comprises a composition containing about 5% or more of crude fat.
[0016]
The barley koji with a milling yield of 80 to 100%, which is the raw material of the present invention, means the outer part of the barley grain, and usually consists of the remainder of the edible wheat in the barley milling process. The composition of these barley straws varies somewhat depending on the type of barley. For example, in the case of Canadian barley (quality B1602), the moisture content is 10.1%, the ash content is 6.1%, the crude protein is 12.1%, and the crude fat is 5. 3% and dietary fiber 56.6%. However, there is no particular limitation on the time of intake and variety.
[0017]
In the present invention, this barley koji is first passed through a 60M sieve to remove a 60M over section with a small hemicellulose content in the total dietary fiber content, and a 60M sieve passage section with a high hemicellulose content (total dietary fiber content is 40% or more). And the hemicellulose content in the total dietary fiber content is 60% or more). By further pulverizing as necessary to adjust the particle size, the barley-derived gastrointestinal function improvement and cholesterol elevation inhibitor of the present invention can be obtained. In addition, the amount of dietary fiber as used in the field of this invention means the indigestible polysaccharide measured by Southgate method.
[0018]
Moreover, in this invention, the health food containing a digestive tract function improving agent and a cholesterol raise inhibitor can be obtained by adding this substance to various foodstuffs. Examples of such foods and beverages include bread and confectionery such as bread, rice crackers, cakes, biscuits and cookies, noodles such as buckwheat, udon and Chinese noodles, prepared dishes such as tempura and fries, and dumplings. Things are raised.
[0019]
In this case, the amount of the substance added to the food is preferably 13% (weight) or more in order to sufficiently impart the effect of improving gastrointestinal function and the effect of suppressing cholesterol elevation.
[0020]
The substance of the present invention can also be used as it is as a health food having an effect of improving gastrointestinal function and an effect of suppressing cholesterol elevation.
[0021]
In the present invention, a substance having a gastrointestinal function improving effect and a cholesterol elevation suppressing effect can be obtained by a very simple process of separating by a sieve. Further, since there is no need to use an alkali, a deproteinizing agent, etc., there is no possibility that a treatment agent or a drug harmful to the human body remains.
[0022]
In the present invention, among the barley koji categories with a yield of 80 to 100% when the barley is refined, the 60M sieve passage category is important, and clearly has an effect of improving gastrointestinal function and increased cholesterol as compared with commercially available wheat bran. An inhibitory effect is observed.
[0023]
However, in the 60M sieve over category, no significant difference is observed compared to commercial wheat bran. The reason for this is that regarding the gastrointestinal function improving effect, the water retention of hemicellulose contained in the passage section of the 60M sieve is very high, the lipid content including the fat-soluble functional component is low, and It can be easily used.
[0024]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, embodiments of the present invention will be described in detail based on examples.
[0025]
【Example】
Rokujo barley (variety B1602) harvested in Canada in 1995 was refined to 80% yield with a RM-5C grinding mill and a RM-3C grinding mill (both manufactured by Satake Corporation). The barley koji component was fractionated into a 30M sieve (aperture 0.5 mm) over section and a 60M sieve (aperture 0.25 mm) passage section using a micro shifter (Dalton). The former was Barley ran 30 (hereinafter abbreviated as BB-30) and the latter was Barley ran 60 (hereinafter abbreviated as BB-60). Commercial wheat bran (hereinafter abbreviated as WB) used in the following test examples was used. In order to remove the influence on the physiological effect due to the difference in particle size, the above samples were all pulverized with a polytron (manufactured by Hosokawa Micron Corporation) so as to pass through a 100M sieve (aperture 0.15 mm). Table 1 shows the component analysis results of each sample.
[0026]
[Table 1]
Figure 0003661044
<Test Example> In order to examine the effect of improving the digestive tract function and the effect of suppressing cholesterol elevation of the sample obtained in Example 1, an animal experiment using rats was conducted.
(A) The feed composition used in the experimental feed experiment is shown in Table 2. The experimental groups were a fiber-free (hereinafter abbreviated as FF) group and a cellulose powder (hereinafter abbreviated as CP, manufactured by Toyo Roshi Kaisha Co., Ltd.) 5 w / w% mixed group as controls, BB-30 group, BB-60. The group and WB group were used as experimental feeds each containing 5 w / w% at the dietary fiber level. In addition, each group of BB-30, BB-60, and WB was adjusted so that the levels of protein, lipid, and starch in the feed would be equal from the general component values.
[0027]
[Table 2]
Figure 0003661044
(B) Animal breeding method Using 4-week-old Sprague-Dawley rats (initial weight 70-85 g, manufactured by Tokyo Experimental Animal Co., Ltd.), using a basic sample based on the AIN-76 composition for 7 days, and following the body weight Each group was divided into 5 groups, 7 animals, and used for the experiment. Rats were placed in individual gauges made of stainless steel, and the interior of the four zones was adjusted to 23 ± 1 ° C., humidity 50 ± 10%, and 12-hour light / dark cycle (light period 8:00 to 20:00). Each feed group and water (tap water) shown in Table 2 were ad libitum, and this breeding was carried out for 28 days.
(C) Analysis and statistical processing method The gastrointestinal transit time (hereinafter abbreviated as GTT) was measured twice on the 8th and 15th days after the start of the breeding. That is, it was fasted from 8:00 to 20:00, and a diet containing 0.5 (w / w)% of carmine as a marker was administered. It was carried out by checking the presence or absence of pigment in feces every 30 minutes and measuring the time required for the marker to be confirmed for the first time (result is the average value of two times).
[0028]
The amount of feces was measured every day for 5 days before the end of this breeding. After measuring the amount of feces, vacuum lyophilization treatment was performed, and the amount of dry feces was measured.
[0029]
In order to examine the effect of each feed group on the characteristics of the digestive tract, the animals were fasted for 3 hours after the breeding period and laparotomy was performed under Nembutal anesthesia. The cecum was quickly taken out and weighed, and a part of the contents was homogenized with ultrapure water, and the pH was measured with a glass electrode. The remaining contents were frozen in liquid nitrogen and stored at −80 ° C., and short-chain fatty acids were quantified with a gas chromatograph (GC-12A, manufactured by Shimadzu Corporation) by the method of Deschner et al.
[0030]
To examine the effect of each feed group on liver cholesterol levels, after laparotomy, the liver was washed with ice-cold physiological saline, and lipids were extracted according to the Folch method performed in a chloroform: methanol mixture (2: 1). Total cholesterol was measured by the method of Zak et al., Triglyceride was measured by the method of Danno et al., And phospholipid was measured by the Bartlett method. For the test of the difference between the average values of these analytical values, a SAS computer package was used, the numerical values were analyzed by one-way analysis of variance (one-way ANOBA), and the test of significant difference was performed by Duncan's multiple range test. The results are shown in Tables 3 to 6.
[0031]
[Table 3]
Figure 0003661044
[0032]
[Table 4]
Figure 0003661044
[0033]
[Table 5]
Figure 0003661044
[0034]
[Table 6]
Figure 0003661044
(D) Explanation on results 1) Explanation on improvement of gastrointestinal function Table 3 and Table 4 show the effects of each test group on feces volume, fecal water content and content transit time in the intestine, and characteristics of the caecum. Table 5 shows the effect on the short-chain fatty acids in the contents. The most characteristic difference was the pH of the cecal contents, compared to the FF group, the CP group and the BB-30 group were hardly changed, whereas the BB-60 group was significantly different from the cecal contents. The pH dropped. The degree was also significant in comparison with the WB group. Although there was no significant difference in the cecal content weight as compared with the WB group and the BB-30 group, the BB-60 group had the largest number (Table 4). One of these causes is that the difference in the component characteristics between BB-60 and other test groups affects the amount of fermentation products (for example, substances that lower the pH such as short-chain fatty acids) ( Table 5). In other words, the BB-60 group contains more substances that are easily decomposed and fermented by intestinal bacteria than the other test groups, and at the same time, there is a possibility that the planting of intestinal bacteria is promoted.
[0035]
Furthermore, when considering the balance between the amount of feces and the water content of feces, the BB-60 group had the highest amount of water in the feces compared to the other test groups. This indicates that the BB-60 group has high water retention. As for GTT, the BB-60 group is significantly shortened compared to the FF group and the CP group, and of course, the BB-30 group is significantly different from the BB-30 group, but is not significantly different from the WB group. Even it was shortened (Table 3). Although the influence of dietary components on GTT is complex, it is generally said that dietary fiber with high water retention increases the amount of feces and shortens GTT. Therefore, it is possible that the water retention of the BB-60 group itself is sufficiently common as a factor that shortened the GTT most compared with the other test groups. Further, in an experiment in which SCFA was directly administered into the colon, butyric acid was found to be most effective in contracting strength (which is said to be correlated to some extent with GTT). Based on this and this result (Table 5), it can be seen that the composition of short-chain fatty acids, which are metabolites of dietary fiber in the BB-60 group, clearly contributes to shortening of GTT.
[0036]
From the above results, it can be seen that the BB-60 group is clearly superior to the other test groups in improving gastrointestinal function, and the effect is equivalent to or higher than that in the WB group. It was.
[0037]
2) Explanation for suppressing cholesterol elevation Table 6 shows the effect of each test group on liver cholesterol. In the amount of triglycerides, the BB-60 test group was significantly lower than the other test groups. In addition, the total cholesterol level was lower than that of other test groups. It is said that the amount of short chain fatty acid produced and the amount of cholesterol have a relatively negative correlation. From this, it was considered that the reason why the BB-60 group is superior in suppressing cholesterol elevation compared to WB is that the production amount of short chain fatty acids is large (Table 5).
[0038]
From the above results, it was found that the BB-60 group had a more excellent cholesterol elevation inhibitory effect than the WB group.
[0039]
【The invention's effect】
According to the present invention, digestion that can be produced in a simpler process without using a dangerous treatment agent or chemical, using barley straw containing abundant dietary fiber as in wheat bran. Tube function improvement and cholesterol elevation inhibitor, and health food containing the same can be provided at a very low cost.

Claims (2)

食用大麦を製造するに際し発生した搗精歩留り80〜100%の区分である大麦糠を、ふるい等で粒度分けしてその粒度を60M篩(目開き0.25mm)通過の区分としてなることを特徴とする大麦由来の消化管機能改善およびコレステロール上昇抑制剤。It is characterized in that barley koji, which is a classification of 80% to 100% of the yield of mash produced during the production of edible barley, is divided into particles by sieving, etc., and the particle size is classified as passing through a 60M sieve (mesh 0.25 mm). An improved barley-derived gastrointestinal function improvement and cholesterol elevation inhibitor. 大麦糠が粗脂肪を約5%以上含有する組成からなることを特徴とする請求項1に記載の大麦由来の消化管機能改善およびコレステロール上昇抑制剤。The barley-derived gastrointestinal tract function improvement and cholesterol elevation inhibitor according to claim 1, wherein the barley koji has a composition containing about 5% or more of crude fat.
JP32438496A 1996-12-04 1996-12-04 Barley-derived gastrointestinal function improvement and cholesterol elevation inhibitor Expired - Fee Related JP3661044B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP32438496A JP3661044B2 (en) 1996-12-04 1996-12-04 Barley-derived gastrointestinal function improvement and cholesterol elevation inhibitor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP32438496A JP3661044B2 (en) 1996-12-04 1996-12-04 Barley-derived gastrointestinal function improvement and cholesterol elevation inhibitor

Publications (2)

Publication Number Publication Date
JPH10165120A JPH10165120A (en) 1998-06-23
JP3661044B2 true JP3661044B2 (en) 2005-06-15

Family

ID=18165198

Family Applications (1)

Application Number Title Priority Date Filing Date
JP32438496A Expired - Fee Related JP3661044B2 (en) 1996-12-04 1996-12-04 Barley-derived gastrointestinal function improvement and cholesterol elevation inhibitor

Country Status (1)

Country Link
JP (1) JP3661044B2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002097203A (en) * 2000-09-22 2002-04-02 Asahi Denka Kogyo Kk β-glucan extraction method
JP2007254464A (en) * 2006-02-24 2007-10-04 Sanwa Shiyurui Kk Cholesterol lowering promotion composition comprising water-insoluble components contained in barley fermented as an active ingredient
US20100062089A1 (en) * 2007-03-13 2010-03-11 Sapporo Breweries Limited Ameliorating or prophylactic agent for metabolic syndrome, and beverage, food and feed each comprising the same
JP5766192B2 (en) * 2010-08-17 2015-08-19 キリン株式会社 Novel plant-derived immunostimulant
JP5809763B1 (en) * 2015-02-27 2015-11-11 清一郎 伊東 Barley-derived polyphenol production method, barley outer layer recovery device, and barley outer layer recovery method
JP2019198281A (en) * 2018-05-17 2019-11-21 花王株式会社 Intestinal flora-improving food and drink
JP2021136923A (en) * 2020-03-04 2021-09-16 株式会社東洋新薬 Composition
WO2022114320A1 (en) * 2020-11-30 2022-06-02 농업회사법인 유한회사 영인바이오 Method for manufacturing noodle snack for companion animals by using fermented barley bran and noodle snack manufactured thereby

Also Published As

Publication number Publication date
JPH10165120A (en) 1998-06-23

Similar Documents

Publication Publication Date Title
Johnson et al. Dietary fibre and related substances
Williams et al. “Dietary fibre”: moving beyond the “soluble/insoluble” classification for monogastric nutrition, with an emphasis on humans and pigs
Christa et al. Buckwheat grains and buckwheat products–nutritional and prophylactic value of their components–a review
McDougall et al. Plant cell walls as dietary fibre: range, structure, processing and function
Sangnark et al. Chemical, physical and baking properties of dietary fiber prepared from rice straw
US5658571A (en) Process for extraction and use of fenugreek (Trigonella foenumgraecum)
Kaur et al. Comparison of dietary fibers obtained from seven Indian cereal grains
Di Cairano et al. Functional cereal-based bakery products, breakfast cereals, and pasta products
Lim et al. Potential functional byproducts from guava purée processing
JP3661044B2 (en) Barley-derived gastrointestinal function improvement and cholesterol elevation inhibitor
Zwer Oats: characteristics and quality requirements
JP6073581B2 (en) Rice with rice flour-like flavor and rice flour
Mabrouki et al. Chemical, functional and nutritional characteristics of raw, autoclaved and germinated fenugreek seeds
Karunanithi et al. Valorization of betel leaves byproducts: A novel ingredient for sustainable cookie development
Astorga-Gaxiola et al. Effect of Traditional Nixtamalization and Extrusion Processes on Bioaccessibility and Antioxidant Activity of Phenolic Compounds in Blue Maize Tortillas During In Vitro Fermentation by Human Colonic Microbiota
JP4480173B2 (en) Oat granule with high concentration of β-glucan
Kulathunga et al. Introduction to rice bran arabinoxylan compound
Caliskan et al. Chemical properties of left over coconut pulp gluten free flour
JPH0728697B2 (en) Low-fat edible wheat bran and fiber foods
WO2004084636A1 (en) Food composition, functional pasta product with high fibre content derived therefrom and process for the production thereof
Segura-Campos et al. Fiber residues from Canavalia ensiformis L. seeds with potential use in food industry.
Prakash et al. Alpha and beta amylase activity of Fagopyrum esculentum (Buckwheat): a medicinal plant
Canja et al. Dietary fiber role and place in baking products
EA001653B1 (en) Drug composition for regulation of bowel activity, containing linseed crush and metho for its production.
Pichaiyongvongdee et al. Evaluation of extraction methods of dietary fiber from pomelo juice byproducts and particle size distribution on the physicochemical and functional properties

Legal Events

Date Code Title Description
A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20040813

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20041028

A521 Written amendment

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20041210

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20050202

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20050307

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110401

Year of fee payment: 6

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110401

Year of fee payment: 6

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110401

Year of fee payment: 6

S531 Written request for registration of change of domicile

Free format text: JAPANESE INTERMEDIATE CODE: R313532

R371 Transfer withdrawn

Free format text: JAPANESE INTERMEDIATE CODE: R371

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110401

Year of fee payment: 6

S531 Written request for registration of change of domicile

Free format text: JAPANESE INTERMEDIATE CODE: R313532

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110401

Year of fee payment: 6

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20140401

Year of fee payment: 9

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees