JP3662556B2

JP3662556B2 - Processing method for crushed samples

Info

Publication number: JP3662556B2
Application number: JP2002208108A
Authority: JP
Inventors: 修二安井
Original assignee: YASUI KIKAI CORPORATION
Current assignee: YASUI KIKAI CORPORATION
Priority date: 2002-07-17
Filing date: 2002-07-17
Publication date: 2005-06-22
Anticipated expiration: 2022-07-17
Also published as: JP2004053301A

Description

【０００１】
【発明の属する技術分野】
本発明は、植物の組織や種子類、動物の組織、プラスチック材料、鉱物材料などの試料を化学的に分析・分画分離するために、破砕容器に試料と破砕媒体とを収容し、振動を加えて破砕媒体により試料を破砕した後、遠心分離する破砕試料の処理方法に関するものである。
【０００２】
【従来の技術】
上記のような試料を化学的に分析・分画するためには、試料を均一に破砕する必要がある。試料を破砕するために破砕装置が用いられ、試料と破砕媒体とを収容した破砕容器に振動を加えることにより、試料に破砕媒体が衝突することによる圧縮と回転による磨砕とによって試料を破砕する。前記破砕装置として本願出願人は特公平６−３６７３２号をはじめとして複数の装置を提案している。
【０００３】
図８は、特願２０００−１０４３４３号として提案した破砕装置の構成を示すもので、回転軸８にその軸芯に対して軸芯が傾斜した傾斜軸体１１を設け、傾斜軸体１１に相対回転自在に環状保持体２０を外嵌させると共に、この環状保持体２０に取り付けられた磁石１６と、これに対極する固定磁石１８とにより環状保持体２０の回転を弾性的に拘束し、環状保持体２０の外周部に破砕媒体３２と試料とを収容した細長い破砕容器３０を環状保持体２０の軸芯と平行な姿勢で保持できるように構成したものである。前記回転軸８を図外のモータにより回転駆動すると、破砕容器３０にはその軸芯方向の比較的長い行程の主往復移動とそれに直交する方向の比較的短い行程の副往復移動とが組み合わされた８の字状の往復振動が加わり、破砕媒体３２が相対回転しながら破砕容器３０の底部に衝突することにより、破砕容器３０が乳鉢、破砕媒体３２が乳棒のように作用して、試料が植物組織や動物組織、あるいはプラスチック材料や鉱物材料であっても効率的に破砕することができる。
【０００４】
前記破砕容器３０は、図９に示すように、細長い円筒容器の開口部外周にネジ３０ａが形成され、開口部に蓋体３１を螺合して密閉できるように構成されている。この破砕容器３０は、ポリカーボネイト、ポリプロピレン等の材料を用いて樹脂成形により製作される。
【０００５】
前記破砕容器３０に試料と共に収容される破砕媒体３２は、図１０に示すように、破砕容器３０の内径Ｄより大きい長さＬの単一部材により構成されており、一端部に破砕容器３０の底部形状に対応した形状の先端部３２ａが形成されている。また、破砕媒体３２の外径ｄは破砕容器３０の内径Ｄに対して２ｍｍ以下、内径ｄが小さい場合には１ｍｍ以下に設定される。また、破砕媒体３２の先端部３２ａは、図１０（ｂ）（ｃ）に示すように、放射状又は螺旋状に１又は複数の溝３４を形成したものを用いることができ、試料が植物材料などである場合に、凍結乾燥しなくても切断され難い繊維質を効果的に切断することができる。この破砕媒体３２の材料は、ステンレス鋼や炭素鋼が一般的に用いられる。
【０００６】
破砕容器３０及び破砕媒体３２の組み合わせは、図１１及び図１２に示すように、破砕容器３０の底部３５を球面に形成し、これに対応させて先端部３２ａを球面に形成した破砕媒体３２を用いることもできる。
【０００７】
上記破砕容器３０及び破砕媒体３２を用いて破砕装置により試料を破砕し、破砕された試料を分析に用いる場合には、破砕後の破砕容器３０内に緩衝液を注入して遠心分離することがなされる。破砕容器３０中に緩衝液を注入してシェークすると、破砕により破砕容器３０の壁面や破砕媒体３２の表面に付着した破砕試料も緩衝液に洗い流されて緩衝液中に懸濁するので、破砕容器３０から破砕媒体３２を取り出し、破砕容器３０を遠心分離にかけると上清と沈殿とに分離することができ、上清の吸光度測定等の手段により分析ができる。
【０００８】
【発明が解決しようとする課題】
しかしながら、破砕媒体を破砕容器から取り出すことは容易でなく、破砕媒体を取り出す際に異物を混入させる恐れもある。また、破砕後に破砕媒体を取り出すと、破砕媒体には破砕された試料が付着しており、試料の回収量が減少するばかりでなく、試料が飛散する恐れもある。
【０００９】
本発明は上記課題に鑑みて創案されたもので、破砕容器からの破砕媒体の取り出しを容易にすると共に、破砕後の遠心分離処理を容易にする破砕試料の処理方法を提供することを目的とする。
【００１０】
【課題を解決するための手段】
本願の第１発明に係る破砕試料の処理方法は、有底筒状体の開口部を蓋体により開閉自在に閉じる破砕容器中に試料と破砕媒体とを収容し、振動を加えて試料を破砕した後、破砕容器中に緩衝液を注入し、破砕容器の外部から強磁性体で形成された前記破砕媒体を磁石により吸着し、破砕媒体を吸着した状態の磁石を蓋体に移動させ、破砕媒体を蓋体の内側に吸着保持した状態にして遠心分離処理した後、蓋体を開くと同時に破砕媒体を取り出し、破砕容器から緩衝液及び破砕された試料を取り出すことを特徴とする。
【００１１】
本願の第１発明によれば、磁石により破砕媒体を蓋体側に吸着保持した状態にして遠心分離することができるので、緩衝液に懸濁した試料は上清と沈殿物とに円滑に分離され、しかも破砕媒体を取り出す作業を行うことなく遠心分離ができる。
【００１３】
また、本願の第２発明は、有底筒状体の開口部を筒状部の外径より大きな外径の蓋体により開閉自在に閉じる破砕容器中に試料と破砕媒体とを収容し、振動を加えて破砕試料を破砕した後、破砕容器中に緩衝液を注入して遠心分離する破砕試料の処理方法において、前記破砕容器の筒状部の直径に対応する開口部が複数に形成された板状体を着磁した容器保持台に、緩衝液注入後の破砕容器を各開口部に挿入することにより、強磁性体で形成された前記破砕媒体を容器保持台の磁性により蓋体の下側に吸着保持した状態にして遠心分離することを特徴とするもので、容器保持台に複数の破砕容器を保持し、破砕媒体を蓋体の下側に吸着した状態で遠心分離することができ、複数の破砕容器を同時に処理することができる。
【００１６】
【発明の実施の形態】
図１は、破砕試料の処理方法を手順を追って略図示したもので、以下に各処理手順を説明する。尚、図に示すＳ１，Ｓ２…は処理手順を示すステップ番号であって、本文に添記する番号と一致する。
【００１７】
まず、破砕容器３０中に破砕する試料５０と破砕媒体３２とを投入して、破砕容器３０の開口部を蓋体３１により閉じ（Ｓ１）、この破砕容器３０を前述した破砕装置にセットして試料５０を破砕すると（Ｓ２）、破砕容器３０中に破砕された試料５０が破砕媒体３２と共にに入った状態となる（Ｓ３）。単に試料５０を破砕するだけでよい場合は、この状態から蓋体３１を開いて破砕容器３０から破砕された試料５０を破砕媒体３２と共に取り出せばよい。
【００１８】
破砕された試料５０を分析などに供する場合には、蓋体３１を開いて破砕容器３０内に所定量の緩衝液５２を注入する（Ｓ４）。緩衝液５２を注入して蓋体３１を閉じ、破砕容器３０をシェークすると、破砕容器３０の壁面や破砕媒体３２の表面に付着した試料５０が洗い出されて緩衝液に破砕された試料５０が懸濁した懸濁液５３が得られる（Ｓ５）。
【００１９】
次に、磁石５１により破砕容器３０の外部から懸濁液５３中にある破砕媒体３２を吸着する（Ｓ６）。破砕媒体３２はステンレス鋼や炭素鋼等の強磁性体で形成されているので、磁石５１によって容易に吸着保持できるので、破砕媒体３２を吸着した磁石５１を蓋体３１側に移動させて破砕媒体３２を蓋体３１の内側に吸着保持した状態にする（Ｓ７）。磁石５１と破砕媒体３２とは蓋体３１を介して引き合った状態になるので安定した保持状態が得られ、この状態で遠心分離の処理を行う（Ｓ８）。
【００２０】
遠心分離によって懸濁液５３は上清５４と沈殿物５５とに分離される（Ｓ９）。破砕容器３０から蓋体３１を取り外すと、蓋体３１と共に破砕媒体３２が取り出される（Ｓ１０）。破砕容器３０には上清５４及び沈殿５５だけが残るので、分析等の処理は容易に行うことができる。
【００２１】
前述した破砕装置は複数の破砕容器３０に対して同時に破砕処理できるので、破砕処理された複数の破砕容器３０を効率よく処理するために、図２に示すように、複数の破砕容器３０を同時処理するための容器保持台６０を用いることができる。
【００２２】
容器保持台６０は、破砕容器３０の外径に対応する容器収納穴６１が複数に形成された鉄系材料の板材で、着磁されて全体が磁性を帯びている。上記処理手順におけるステップＳ４の緩衝液５２を注入する処理手順の後、シェークして破砕された試料５０を洗い出した破砕容器３０を容器保持台６０の容器収納穴６１に挿入すると、図示するように着磁された容器保持台６０によって強磁性体である破砕媒体３２は容器保持台６０に吸着されて蓋体３１側に保持された状態になる。この容器保持台６０に破砕容器３０を収容した状態で遠心分離すると、破砕された試料５０と緩衝液５２とが懸濁した懸濁液５３は上清５４と沈殿５５とに分離できるので、各破砕容器３０は蓋体３１の上に磁石５１を置いて破砕媒体３２を吸着保持した状態にして容器保持台６０から取り出すと、図１に示したステップＳ９と同様の状態が得られるので、破砕容器３０から蓋体３１を開くと、ステップＳ１０に示す如く蓋体３１と共に破砕媒体３２を取り出すことができる。
【００２３】
また、図１に示した処理手順のステップＳ４において、緩衝液５２を注入するために蓋体３１を開くと、破砕された試料５０が乾燥物である場合に、蓋体３１を開いたときや緩衝液５２を注入したときに、破砕されて粉末状になった試料５０が舞い上がる恐れがある。緩衝液５２の注入は注射器やスポイトで行われるので破砕容器３０の開口部を大きく開く必要はない。
【００２４】
そこで、図３に示すように、蓋体３１に緩衝液５２を注入するのに必要な最小限の径で注入孔６６を設け、この注入孔６６をネジ蓋（閉止体）６７で閉じるように構成すると、処理手順ステップＳ４において緩衝液５２を注入する際にネジ蓋６７を開いて小さい注入孔６６から緩衝液５２を注入することができる。この注入孔６６とネジ蓋５２の構成は、ネジ蓋６７の外径が大きく平坦に形成してあるので、磁石５１により破砕媒体３２を吸着保持するのに障害とはならない。
【００２５】
また、図４に示すように、蓋体３１に注入口９０を設け、蓋体３１の内側に軟質のシリコンセプタム（閉止体／軟質体）６９を配設すると、緩衝液５２の注入時に蓋を開くことなく注入することができる。注入手段（注射器やスポイト）を注入口９０から挿入し、その先端をシリコンセプタム６９に圧入すると破砕容器３０内に注入手段の先端を挿入することができるので、緩衝液５２を破砕容器３０内に注入することができる。緩衝液の注入後は注入手段を引き抜くとシリコンセプタム６９は弾性復帰により注入手段が通過した通路を塞ぐので、破砕容器３０内から外部方向への流通は阻止され、破砕容器３０内に収容された試料５０や緩衝液５２は蓋体３１を開かない限り逸脱しない。
【００２６】
小さい破砕容器３０では、図５に示すように、複数の破砕容器３０に対して複数の蓋体３１を連結した連結蓋体７０で各破砕容器３０を連結した状態にして破砕処理を行なうことがなされる。複数の破砕容器３０は、各破砕容器３０に試料と破砕媒体３２とを収納し、連結蓋体７０の各蓋体３１で破砕容器３０それぞれを閉じ、図６に示すように、容器ホルダ７１に列設配置されて破砕装置にセットされ、多数の破砕容器３０について一括した破砕処理がなされる。
【００２７】
このような破砕容器３０の構成においては、図７に示すように、破砕処理後に容器ホルダ７１に収容された各破砕容器３１を覆う大きさの磁石７２を蓋体３１上に置くと、各破砕容器３０中の破砕媒体３２は磁石７２に磁気吸引されてそれぞれの蓋体３１の内側に吸着保持される。この状態で磁石７２を持ち上げると破砕容器３０を容器ホルダ７１から取り出せるので、破砕容器３０を蓋体３１から外すと蓋体３１と破砕媒体３２は磁石７２側に残り、破砕容器３０の中の破砕された試料を取り出すことができる。
【００２８】
上記構成における磁石７２は、容器ホルダ７１に収納された全ての破砕容器３０を覆う大きさのものとしているが、連結蓋体７０で連結された複数の破砕容器３０を覆う単位のサイズとすることができ、破砕容器３０の取り外しはより容易となる。
【００３０】
【発明の効果】
本願第１発明によれば、破砕媒体を取り出す作業を行うことなく遠心分離ができる。
【００３２】
また、本願第２発明によれば、容器保持台に複数の破砕容器を保持し、破砕媒体を蓋体の下側に吸着した状態で遠心分離することができ、複数の破砕容器を同時に処理することができる。
【図面の簡単な説明】
【図１】破砕試料の処理手順を示す工程図。
【図２】保持台に破砕容器を挿入した状態を示す断面図。
【図３】緩衝液注入用の孔を設けた蓋体の断面図。
【図４】緩衝液注入用の軟質体を設けた蓋体の断面図。
【図５】複数の破砕容器を連結蓋体で連結した態様を示す斜視図。
【図６】同上破砕容器を容器ホルダに収納した状態を示す斜視図。
【図７】同上破砕容器から破砕媒体を取り出す方法を示す側面図。
【図８】破砕装置の構成を示す断面図。
【図９】破砕容器の断面図。
【図１０】破砕媒体の各種例の正面図。
【図１１】破砕容器の断面図。
【図１２】破砕媒体の各種例の正面図。
【符号の説明】
３０破砕容器
３１蓋体
３２破砕媒体
５０試料
５１、７２磁石
５２緩衝液
６０保持台
６１容器収納穴
６６注入孔
６７ネジ蓋
６９シリコンセプタム（軟質体）[0001]
BACKGROUND OF THE INVENTION
In order to chemically analyze and fractionate samples such as plant tissues and seeds, animal tissues, plastic materials, mineral materials, etc., the present invention contains a sample and a crushing medium in a crushing container, and vibrates. after crushing a sample by crushing medium added, are those concerning the processing how crushing sample centrifuged.
[0002]
[Prior art]
In order to chemically analyze and fractionate the sample as described above, it is necessary to uniformly crush the sample. A crushing device is used to crush the sample, and by crushing the crushing container containing the sample and the crushing medium, the sample is crushed by compression caused by the crushing medium colliding with the sample and grinding by rotation. . As the crushing apparatus, the applicant of the present application has proposed a plurality of apparatuses including Japanese Patent Publication No. 6-36732.
[0003]
FIG. 8 shows the configuration of the crushing apparatus proposed as Japanese Patent Application No. 2000-104343. The rotating shaft 8 is provided with an inclined shaft body 11 whose axis is inclined with respect to the axis, and is relative to the inclined shaft body 11. The annular holder 20 is externally fitted in a freely rotatable manner, and the rotation of the annular holder 20 is elastically constrained by the magnet 16 attached to the annular holder 20 and the fixed magnet 18 opposite to the magnet 16, thereby holding the annular holder. The elongated crushing container 30 containing the crushing medium 32 and the sample on the outer periphery of the body 20 is configured to be held in a posture parallel to the axis of the annular holder 20. When the rotary shaft 8 is rotationally driven by a motor (not shown), the crushing container 30 is combined with a main reciprocating movement with a relatively long stroke in the axial direction and a sub-reciprocating movement with a relatively short stroke in the direction orthogonal thereto. When the crushing medium 32 collides with the bottom of the crushing container 30 while rotating relatively, the crushing container 30 acts like a mortar and the crushing medium 32 acts like a pestle. Even plant tissues, animal tissues, plastic materials and mineral materials can be efficiently crushed.
[0004]
As shown in FIG. 9, the crushing container 30 is configured such that a screw 30a is formed on the outer periphery of the opening of an elongated cylindrical container, and a lid 31 can be screwed into the opening to be sealed. The crushing container 30 is manufactured by resin molding using a material such as polycarbonate or polypropylene.
[0005]
The crushing medium 32 accommodated in the crushing container 30 together with the sample is constituted by a single member having a length L larger than the inner diameter D of the crushing container 30, as shown in FIG. A tip portion 32a having a shape corresponding to the bottom shape is formed. Further, the outer diameter d of the crushing medium 32 is set to 2 mm or less with respect to the inner diameter D of the crushing container 30, and is set to 1 mm or less when the inner diameter d is small. Further, as shown in FIGS. 10B and 10C, the tip 32a of the crushing medium 32 can be one in which one or a plurality of grooves 34 are formed radially or spirally, and the sample is a plant material or the like. In this case, it is possible to effectively cut the fiber that is difficult to cut without being lyophilized. As the material of the crushing medium 32, stainless steel or carbon steel is generally used.
[0006]
As shown in FIGS. 11 and 12, the combination of the crushing container 30 and the crushing medium 32 includes a crushing medium 32 in which the bottom 35 of the crushing container 30 is formed into a spherical surface, and the tip 32a is formed into a spherical surface corresponding to this. It can also be used.
[0007]
When the sample is crushed by the crushing apparatus using the crushing container 30 and the crushing medium 32 and the crushed sample is used for analysis, a buffer solution may be injected into the crushing container 30 after crushing and centrifuged. Made. When the buffer solution is poured into the crushing container 30 and shaken, the crushing sample adhering to the wall surface of the crushing container 30 or the surface of the crushing medium 32 due to crushing is also washed away in the buffer solution and suspended in the buffer solution. When the crushing medium 32 is taken out from 30 and the crushing container 30 is centrifuged, it can be separated into a supernatant and a precipitate, and can be analyzed by means such as measuring the absorbance of the supernatant.
[0008]
[Problems to be solved by the invention]
However, it is not easy to take out the crushing medium from the crushing container, and foreign substances may be mixed when taking out the crushing medium. Further, when the crushing medium is taken out after crushing, the crushed sample adheres to the crushing medium, and not only the amount of collected sample is reduced but also the sample may be scattered.
[0009]
The present invention was devised in view of the above problems, and an object of the present invention is to provide a method for treating a crushed sample that facilitates the removal of a crushing medium from a crushing container and facilitates a centrifugal separation process after crushing. To do.
[0010]
[Means for Solving the Problems]
The processing method of the crushing sample which concerns on 1st invention of this application accommodates a sample and a crushing medium in the crushing container which closes the opening part of a bottomed cylindrical body so that opening and closing is possible, and crushes a sample by applying a vibration. After that, a buffer solution is injected into the crushing container, the crushing medium formed of a ferromagnetic material is adsorbed from the outside of the crushing container with a magnet, and the magnet with the crushing medium adsorbed is moved to the lid body to crush After centrifugation with the medium adsorbed and held inside the lid, the crushing medium is taken out simultaneously with opening the lid, and the buffer solution and the crushed sample are taken out from the crushing container .
[0011]
According to the first invention of this application, since the crushing medium can be adsorbed and held on the lid side by the magnet and centrifuged, the sample suspended in the buffer is smoothly separated into the supernatant and the precipitate. In addition, centrifugation can be performed without taking out the crushing medium.
[0013]
Further, the second invention of the present application accommodates the sample and the crushing medium in a crushing container in which the opening of the bottomed cylindrical body is opened and closed by a lid having an outer diameter larger than the outer diameter of the cylindrical part, after crushing the crushed sample by adding, in the processing method of crushing the sample to centrifugation by injecting buffer in broken砕容unit, opening corresponding to the diameter of the cylindrical portion of the crushing chamber is formed in a plurality and the container holding the magnetized plate-like body, buffer solution by crushing the container after injection for insertion into the openings, the lid the grinding media formed of a ferromagnetic material of a magnetic container holder It is characterized by centrifuging in a state of being adsorbed and held on the lower side, and holding a plurality of crushing containers on a container holding stand, and centrifuging with a crushing medium adsorbed on the lower side of the lid And a plurality of crushing containers can be processed simultaneously.
[0016]
DETAILED DESCRIPTION OF THE INVENTION
FIG. 1 schematically shows a processing method of a crushed sample, step by step, and each processing procedure will be described below. In the figure, S1, S2,... Are step numbers indicating processing procedures, and coincide with numbers appended to the text.
[0017]
First, the sample 50 to be crushed and the crushing medium 32 are put into the crushing container 30, the opening of the crushing container 30 is closed by the lid 31 (S1), and the crushing container 30 is set in the crushing apparatus described above. When the sample 50 is crushed (S2), the sample 50 crushed in the crushing container 30 enters a state together with the crushing medium 32 (S3). When it is sufficient to simply crush the sample 50, the lid 50 is opened from this state, and the sample 50 crushed from the crushing container 30 may be taken out together with the crushing medium 32.
[0018]
When the crushed sample 50 is used for analysis or the like, the lid 31 is opened and a predetermined amount of buffer solution 52 is injected into the crushed container 30 (S4). When the buffer solution 52 is injected, the lid 31 is closed, and the crushing container 30 is shaken, the sample 50 adhered to the wall surface of the crushing container 30 or the surface of the crushing medium 32 is washed out and the sample 50 crushed into the buffer solution is obtained. A suspended suspension 53 is obtained (S5).
[0019]
Next, the crushing medium 32 in the suspension 53 is adsorbed by the magnet 51 from the outside of the crushing container 30 (S6). Since the crushing medium 32 is formed of a ferromagnetic material such as stainless steel or carbon steel, it can be easily adsorbed and held by the magnet 51. Therefore, the magnet 51 adsorbing the crushing medium 32 is moved to the lid body 31 side, and the crushing medium 32 is moved. 32 is brought into a state of being sucked and held inside the lid 31 (S7). Since the magnet 51 and the crushing medium 32 are in a state of being attracted via the lid 31, a stable holding state is obtained, and the centrifugal separation process is performed in this state (S8).
[0020]
The suspension 53 is separated into a supernatant 54 and a precipitate 55 by centrifugation (S9). When the lid body 31 is removed from the crushing container 30, the crushing medium 32 is taken out together with the lid body 31 (S10). Since only the supernatant 54 and the precipitate 55 remain in the crushing container 30, processing such as analysis can be easily performed.
[0021]
Since the crushing apparatus described above can simultaneously crush a plurality of crushing containers 30, in order to efficiently process the crushing crushing containers 30, as shown in FIG. A container holder 60 for processing can be used.
[0022]
The container holding table 60 is a plate of iron-based material in which a plurality of container storage holes 61 corresponding to the outer diameter of the crushing container 30 are formed, and is magnetized so as to be magnetic as a whole. After the processing procedure of injecting the buffer solution 52 in step S4 in the above processing procedure, when the crushing container 30 in which the sample 50 that has been shaken and crushed is washed out is inserted into the container holding hole 61 of the container holding base 60, as shown in the figure. The crushing medium 32, which is a ferromagnetic material, is attracted to the container holding table 60 and held on the lid 31 side by the magnetized container holding table 60. Since the suspension 53 in which the crushed sample 50 and the buffer solution 52 are suspended can be separated into the supernatant 54 and the precipitate 55 by centrifuging in a state where the crushing container 30 is accommodated in the container holding table 60, each When the crushing container 30 is placed on the lid 31 with the magnet 51 placed in a state where the crushing medium 32 is attracted and held and taken out from the container holding stand 60, the same state as step S9 shown in FIG. When the lid 31 is opened from the container 30, the crushing medium 32 can be taken out together with the lid 31 as shown in step S10.
[0023]
Further, in step S4 of the processing procedure shown in FIG. 1, when the lid body 31 is opened to inject the buffer solution 52, when the crushed sample 50 is a dried product, the lid body 31 is opened. When the buffer solution 52 is injected, the sample 50 that has been crushed into a powder form may rise. Since the injection of the buffer solution 52 is performed by a syringe or a syringe, it is not necessary to open the opening of the crushing container 30 widely.
[0024]
Therefore, as shown in FIG. 3, an injection hole 66 having a minimum diameter necessary for injecting the buffer solution 52 into the lid 31 is provided, and the injection hole 66 is closed with a screw lid (closing body) 67. When configured, the buffer solution 52 can be injected from the small injection hole 66 by opening the screw cap 67 when the buffer solution 52 is injected in the processing procedure step S4. The configuration of the injection hole 66 and the screw lid 52 is not an obstacle to attracting and holding the crushing medium 32 by the magnet 51 because the screw lid 67 has a large outer diameter and is formed flat.
[0025]
In addition, as shown in FIG. 4, when an injection port 90 is provided in the lid 31 and a soft silicon septum (closed body / soft body) 69 is provided inside the lid 31, the lid is closed when the buffer solution 52 is injected. Can be injected without opening. When the injection means (syringe or syringe) is inserted from the injection port 90 and the tip of the injection means is press-fitted into the silicon septum 69, the tip of the injection means can be inserted into the crushing container 30, so that the buffer solution 52 is put into the crushing container 30. Can be injected. After the injection of the buffer solution, when the injection means is pulled out, the silicon septum 69 closes the passage through which the injection means has passed by elastic recovery, so that the flow from the inside of the crushing container 30 to the outside is blocked and the silicon septum 69 is accommodated in the crushing container 30. The sample 50 and the buffer solution 52 do not deviate unless the lid 31 is opened.
[0026]
In the small crushing container 30, as shown in FIG. 5, the crushing process can be performed by connecting the crushing containers 30 with the connecting lid 70 connecting the plurality of lids 31 to the plurality of crushing containers 30. Made. The plurality of crushing containers 30 store the sample and the crushing medium 32 in each crushing container 30, and close each crushing container 30 with each lid body 31 of the connection lid body 70, and as shown in FIG. The crushing process is performed for a large number of crushing containers 30 by arranging and arranging them in a crushing apparatus.
[0027]
In such a configuration of the crushing container 30, as shown in FIG. 7, when a magnet 72 having a size covering each crushing container 31 accommodated in the container holder 71 after the crushing process is placed on the lid body 31, The crushing medium 32 in the container 30 is magnetically attracted by the magnet 72 and is attracted and held inside each lid 31. When the magnet 72 is lifted in this state, the crushing container 30 can be taken out from the container holder 71. Therefore, when the crushing container 30 is removed from the lid 31, the lid 31 and the crushing medium 32 remain on the magnet 72 side and crushing in the crushing container 30 is performed. The collected sample can be taken out.
[0028]
The magnet 72 in the above configuration has a size that covers all the crushing containers 30 stored in the container holder 71, but has a unit size that covers a plurality of crushing containers 30 connected by the connecting lid 70. The crushing container 30 can be removed more easily.
[0030]
【The invention's effect】
According to the first invention, it is centrifuged without performing the work of taking out the broken砕媒body.
[0032]
Further, according to the second invention, holds a plurality of crushing containers container holder, the grinding media can be centrifuged in a state of being adsorbed on the underside of the lid, a plurality of crushing containers treated simultaneously can do.
[Brief description of the drawings]
FIG. 1 is a process chart showing a processing procedure for a crushed sample.
FIG. 2 is a cross-sectional view showing a state in which a crushing container is inserted into a holding table.
FIG. 3 is a cross-sectional view of a lid body provided with a buffer solution injection hole.
FIG. 4 is a cross-sectional view of a lid provided with a soft body for buffer injection.
FIG. 5 is a perspective view showing an aspect in which a plurality of crushing containers are connected by a connecting lid.
FIG. 6 is a perspective view showing a state in which the same crushing container is housed in a container holder.
FIG. 7 is a side view showing a method of taking out the crushing medium from the crushing container.
FIG. 8 is a cross-sectional view showing a configuration of a crushing device.
FIG. 9 is a cross-sectional view of a crushing container.
FIG. 10 is a front view of various examples of crushing media.
FIG. 11 is a cross-sectional view of a crushing container.
FIG. 12 is a front view of various examples of crushing media.
[Explanation of symbols]
30 Crushing container 31 Lid 32 Crushing medium 50 Sample 51, 72 Magnet 52 Buffer 60 Holding base 61 Container receiving hole 66 Injection hole 67 Screw lid 69 Silicon septum (soft body)

Claims

The sample and the crushing medium are housed in a crushing container in which the opening of the bottomed cylindrical body is opened and closed freely with a lid. After crushing the sample by applying vibration, a buffer solution is injected into the crushing container and crushing. The crushing medium formed of a ferromagnetic material is adsorbed from the outside of the container with a magnet, the magnet with the crushing medium adsorbed is moved to the lid, and the crushing medium is adsorbed and held inside the lid and centrifuged. After the separation treatment, a crushing sample processing method, wherein the crushing medium is taken out simultaneously with opening the lid, and the buffer solution and the crushed sample are taken out from the crushing container.

After the sample and the crushing medium are housed in a crushing container in which the opening of the bottomed cylindrical body is opened and closed with a lid having an outer diameter larger than the outer diameter of the cylindrical part, and the crushed sample is crushed by applying vibration In the processing method of the crushing sample in which the buffer solution is injected into the crushing container and centrifuged, the container holding the plate-like body formed with a plurality of openings corresponding to the diameter of the cylindrical part of the crushing container By inserting the crushing container after injection of the buffer solution into each opening on the table, the crushing medium formed of a ferromagnetic material is adsorbed and held under the lid by the magnetism of the container holding table and centrifuged. A method for treating a crushed sample characterized by separating.