JP3694091B2 - Preparation of organic acid mineral solution antibacterial agent for bacterial growth inhibition - Google Patents

Description

【００１５】
表１から明らかなように、無添加区では耐熱芽胞菌（バチルス属枯草菌）が活発に増殖しており、この菌は枯草菌であった。
また、無添加区では一般生菌数も増殖しており、あんは腐敗の直前の状態であった。
Ａ剤の添加区では枯草菌の増殖はみられなかった。
【００１６】
〔検証例２〕
つぎに検証例１で増殖した菌の胞子形成を確認し、その培地から胞子を採取して懸濁液を作成し、胞子の発芽にヒートショックを与えるため１００℃で５分間加熱し、その懸濁液を、Ａ剤を表２に示す濃度に希釈混入の培養プレートに接種し、Ａ剤が耐熱芽胞菌の増殖を阻止する効果を試験した。
また再加熱しない懸濁液の接種培地を用意し胞子菌と栄養細胞細菌の増殖試験も併行して実施した。その結果を表２に示す。
【００１７】
【表２】

【００１８】
表２から明らかなように、Ａ剤を混入しない（対象０％）では菌は増殖したが、Ａ剤を０．８％以上混入したプレートではその増殖は見られなかった。なお、再加熱した胞子菌プレートの方は、再加熱することで胞子菌以外の一般生菌が加熱滅菌されたため培地には胞子菌のみが接種されている。一方、非再加熱菌のプレートでは、共存の一般生菌が加熱滅菌されていないため総量として菌コロニーは若干多い傾向が見られた。
【００１９】
表２の結果から、耐熱芽胞菌の胞子発芽の特長はヒートショックを与えることで芽胞の発芽が促され、発芽から栄養細胞への強力な転生と細胞増殖がおもなわれるが、その過程において、Ａ剤の高浸透圧によるミネラルイオンの細胞膜、細胞壁の透過により、細胞基質のミネラル変性、タンパク合成酵素失活など細菌増殖環境に多大な影響を与え、ヒートショックを与えられ、発芽が促された耐熱芽胞菌の増殖が抑えられたことが実証された。
上記のように、耐熱胞子形成菌の枯草菌の増殖阻止は従来、複雑な工程と多くのエネルギー消費をともなっていたが、本発明に係る有機酸ミネラル溶液の一定濃度（０．８％以上）において容易に、正確に阻止することが確認できた。
食品の生産、流通、貯蔵において、本発明の有機酸ミネラル溶液の抗菌剤として利用することにより、保健衛生上有益であり、経済効率は向上し、省エネルギーとなる。
【００２０】
〔検証例３〕
空気中に無数に飛び交っているカビ菌の胞子が物体に付着して繁殖し菌叢としてコロニーを形成する。そこで、胞子の着生後における増殖阻止の効果について切りもちで試験をした。もちは加水が少なく、カビの発生しやすい食品であるので、カビの着生と増殖の確認の試験が容易に実施できる。
試験は同一製造条件の切ちもちについて下記種類の試験品を作成した。
（１） 無処理
（２） Ｂ剤を水で１０倍に希釈した液を切りもちの片面に刷毛で塗布
これらを空気が自由に出入できる同一容器内に収納して２１日間放置し、その過程での状態を観察した。その結果を表３に示す。
【００２１】
【表３】

【００２２】
無処理品に増殖したカビは真菌のペニシリウム属の青カビであった。
この試験は空気が自由に出入りでき、カビの胞子が各試験品へ付着することが確認できる同一容器に試験品を収納して実施しており、Ｂ剤希釈水のミネラルイオンが、胞子着生後、胞子の細胞壁に浸透し、細胞基質のミネラル変性、タンパク合成酵素失活、さらに資化阻害をなし、カビの有機養料菌としての炭水化物、窒素源の栄養摂取をも阻害して成育阻止および増殖阻止していることが確認できた。
このような効果は、安全性の高い抗菌剤を希釈して食品あるいは、器物に直接塗布し、長期間効果を維持するもので、本発明有機酸ミネラル溶液の優れた効果を示すものである。
【００２３】
〔検証例４〕
薯蕷饅頭は米粉、山芋、砂糖を材料に使用する食品で水分が多く、原料由来の細菌類、カビ菌、腐敗菌の着生増殖が早い。Ａ剤を下記内容にて塗布し通常どおり１２分蒸して試験品とした。他にＡ剤を添加しない無処理のものを同一条件で用意しカビ菌の増殖阻止試験をした。
（１）無処理
（２）Ａ剤を水で５倍に希釈し饅頭の外側にスプレーして蒸す
試験品を室内に常温で保存し、カビ発生と増殖阻止の比較試験を実施した。その結果を表４に示す。
【００２４】
【表４】

【００２５】
無処理品のカビは、普通に澱粉加工品に繁殖するアスペルギルス、ユーロチウムとみられる。
比較試験において、同一の保存条件下で、胞子の着生もほぼ同一量であるが、Ａ剤を添加した試験品は抗菌剤がカビ菌の発芽、増殖を阻止していることが実証されている。なお、Ａ剤の作用については検証例３の記載と同じである。
上記検証例から明らかなように、Ａ剤は加熱されてもその効果に減少がなく、加工に際しての温度変化にも影響がなく、安定した効果があり、安全で容易に使用することができ、効果は正確であり長期間持続する。
【００２６】
〔検証例５〕
酵母は主に有用菌として利用されるが、ときには有害菌となることもある。有用菌としては、酵母の増殖、すなわち発酵作用の操作があり、また有害菌としては、酵母の着生による炭酸ガスの発生で包装食品の膨張、破袋、腐敗発酵などがある。この有害作用に有機酸ミネラル溶液を抗菌剤として利用する。
パン酵母作用のパン生地にＢ剤を添加して膨張率の推移について比較試験をし、その結果を表５に示す。
【００２７】
【表５】

【００２８】
酵母は細菌類、カビ菌より大きい単細胞微生物であり、細胞壁の組成がグルカン、マンナンなど強固なため、一般に抗菌剤の利用が難しいといわれ、抗菌剤の添加量は細菌類、カビ菌への使用量よりも多い量が必要であった。このような状況に対し、有機酸ミネラル溶液は高浸透圧の作用により、細胞壁、細胞質膜を浸透して、細胞内容の原形質に影響し阻害作用を与え、酵母の発酵を阻止、遅延する効果が実証された。これは酵母を利用する技術として、あるいは酵母汚染による腐敗増殖阻止としての抗菌剤として優れた作用、効果である。
【００２９】
〔検証例６〕
栄養細胞細菌類へのカルシウムをはじめとするミネラル類の高い浸透圧により、細胞結合水の滲出による自由水の増加と、タンパク質の変性と、細胞分裂の増殖サイクル下での栄養細胞にたいする高浸透圧作用、栄養摂取の阻害すなわち資化阻害で、細菌増殖と離水の関係のなかで抗菌効果をみるため、検証対象として短時間で腐敗しやすいカスタードクリームを使用し、これの一般生菌の増加と離水による抗菌効果をＡ剤を添加したカスタードクリームについて試験をした。その結果を表６に示す。
【００３０】
【表６】

【００３１】
無添加区では細菌の増殖と離水が比例してすすみ、添加区では増殖の阻止が有効にはたらき、添化区では離水がないことが確認できた。
上述した検証例で検証できたように胞子形成菌、カビ、酵母などの成育最適環境下においてでも有機酸ミネラル溶液の抗菌剤としての十分な効果がある。このことは、当然ながらこれらより抵抗性の劣る細菌にはより有効である。それは単に増殖阻止を腐敗と離水の両面の関係でみることでさらに効果を確認できた。
【００３２】
〔検証例７〕
黒カビはぜん息やアレルギーの原因物質ともいわれ、環境衛生のなかで対策が求められているカビ菌である。浴室のタイル目地の黒カビを対象に試験を実施した。
Ｂ剤を局法ワセリンに３％の割合で混合希釈し（以下抗菌剤入りワセリンという）これを試験に供した。
予め黒カビが密生している浴室タイル目地に試験区を設定し、洗剤で洗浄後黒カビ胞子の着床と増殖の試験を実施した。試験区以外の周辺の黒カビはそのままである。
（１）Ａ試験区：洗剤でカビを除去し、ワセリンのみを塗布
（２）Ｂ試験区：洗剤でカビ除去後抗菌剤入りワセリンを塗布
新たなカビの発生までの時間差を計測して抗菌効果を判定した。その結果を表７に示す。
【００３３】
【表７】

【００３４】
Ａ試験区では２５日後に黒カビのコロニーが発生３７日後に周囲と同一の密度に増殖した。一方Ｂ試験区では５８日後に黒カビのコロニーが発生７８日後に周囲と同一となった。
黒カビ（クラドスポリウム類）は、住居のほか器物、農産物などその繁殖は広範囲におよぶ。食品関連の抗菌作用のみでなく、さらに対象を広くし、食品とは条件の異なる居住環境においても、有機酸ミネラル溶液は同様の抗菌作用がある。黒カビの着生に好適な条件下においても、胞子への直接の浸透作用、細胞基質の変性、胞子の発芽、資化阻害により増殖の阻止作用があり、広く生活環境の全般に安全正確な抗菌効果を有する。
【００３５】
〔検証例８〕
ヒトの皮膚に感染する糸状菌を皮膚糸状菌といい、少胞子菌、白癬菌、表皮菌の３属がある。代表的な白癬菌は皮膚に着生増殖し感染症として、シラクモ、ミズムシ、タムシなどの原因となる。この白癬菌にたいする有機酸ミネラル溶液の抗菌試験をした。試験は、
無添加の培地の白癬菌を接種培養、
Ａ剤を０．５％に希釈して添加した添加培地に白癬菌を接種培養、
Ａ剤を１．０％に希釈して添加した添加培地に白癬菌を接種培養
の３種類の培地につきおこない、白癬菌増殖と増殖阻止の状況を観察した。その結果を表８に示す。
【００３６】
【表８】

【００３７】
皮膚糸状菌は増殖の条件がヒトに由来の栄養成分と温度などの条件がともなうため、それに適した栄養成分の培地において培養と増殖阻止の効果を比較した。その結果、有機酸ミネラル溶液が細胞の増殖阻止に有効に作用していることが確認でき、ヒトの表皮付近に生存する感染症につき、無害で抗菌効果のある抗菌剤として優れた効能を発揮することが確認できた。
【００３８】
〔検証例９〕
一般的にｐＨが４．５以下になると細菌の成育が抑制されるが、これは微生物の細胞内のタンパク質が低いｐＨでの強い浸透圧により細胞膜を透過したミネラルにより細胞基質が影響をうけ、そして細菌の増殖機能を阻害し、結果として阻止の作用をする。この作用は、有機酸類は溶液中で一部が解離するが水素イオン濃度が増加すると解離の比率が減り非解離分子が細菌の細胞膜を透過してタンパク質変性をひきおこすことによる。
【００３９】
クエン酸の０．５％水溶液のｐＨは２．８であるが、Ａ剤を１％添加した水溶液にクエン酸０．５％を加えても、ｐＨ４．８でありｐＨの低下はない。これは、低いｐＨ４．５以下でのみ実現していた高い浸透圧の作用あるいは細菌類の増殖阻止作用が、強酸性域に限定されないという利点をもたらすものである。すなわち、有機酸ミネラル溶液は食品にあっては食味（酸っぱさ）への影響、ヒトの皮膚では過度の酸刺激の緩和、環境では機器の酸化、腐食を改善する。またこれが、細菌の細胞膜、細胞壁を浸透しうる十分な浸透圧をもつ理由である。
【００４０】
表９は本発明の製造法で作成した有機酸ミネラル溶液を、水で希釈した水溶液の希釈割合とｐＨの推移を測ったものである。この表９から明らかなように、有機酸ミネラル溶液を希釈した水溶液は弱酸性を維持しながらもなおかつ水溶液中の水素イオン濃度がｐＨの上昇の条件であってもｐＨを維持する能力をもつことが確認できる。このように有機酸ミネラル溶液は広範囲にわたる緩衝作用があり、従来の抗菌剤にないすぐれた特徴をもつ。
【００４１】
【表９】

【００４２】
本発明の有機酸ミネラル溶液は、食品に利用されている素材を原材料に使用して製造するため、安全で、広範囲な用途で使用できる抗菌剤を提供することができる。本発明で作成する有機酸ミネラル溶液は、ｐＨ４．５以下に低下させなきとも、従来のこれより低いｐＨ領域下の浸透圧よりもはるかに高い浸透圧を維持し、細菌類の細胞壁、細胞膜を透過する抗菌剤効果をもつ。
そしてミネラルイオン量濃度が高いことは、細胞基質に影響を与え、細胞増殖因子を阻害する結果、細菌類の増殖を阻止する。
さらに細菌類の資化阻害は、カビ胞子の発芽を長期にわたりこれを阻止する効果に現れている。食品では食味に影響することなしに腐敗を防ぎ、省エネルギーをはかり抗生物質の食用動植物への濃厚使用や習慣使用による耐抗菌の弊害を減少するものである。
【００４３】
居住環境においても新規な効果をもち、環境衛生に有効に作用する有機酸ミネラル溶液を提供するものである。
ヒトの健康向上についてもまた資源の損失をも防ぐ効果がある。ヒトの皮膚感染症菌類について本有機酸ミネラル溶液を抗菌剤として使用すると、とくに刺激がなく、体内吸収での安全性もある抗菌剤となる。
本有機酸ミネラル溶液は緩衝作用の範囲が広く緩衝機能があるので多種類の薬剤等の希釈剤として利用することができる。弱酸性域内で、強酸性域の薬剤効果を発揮できるなどのあらたな希釈剤として効果を発揮する。
【００４４】
【発明の効果】
本発明は、抗菌剤の原材料を安全な天然の素材に求め、これを醸造酢酸発酵法により多種類のミネラル（無機塩類）を高濃度に溶解させ、所望ｐＨの有機酸ミネラル溶液を製造することができる優れた効果を有するものである。
また、この有機酸ミネラル溶液を抗菌剤として食物、ヒト、環境中に使用し、従来では容易に実現できなかった真菌類、胞子形成菌類の増殖阻止を、容易に、的確に阻止し良好な生活環境（安全衛生）を保持することができる、優れた効果を有するものである。
【図面の簡単な説明】
【図１】胞子形成細菌の生活環の説明図である。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a method for producing an organic acid mineral solution antibacterial agent that inhibits bacterial growth, and contributes to improving and improving the living environment (health hygiene, residential hygiene environment, food hygiene) as an antibacterial agent, and is safe and comfortable Ensuring a safe life and effective use of resources.
[0002]
[Prior art]
There are many chemically synthesized inorganic and organic products as antibacterial agents that protect food and sanitary environments. Antibiotics, organic acids and salts are also widely used. Moreover, although there exists what melt | dissolved the mineral in the vinegar, the acetic acid amount of a vinegar is 5 to 10%, and the dissolved amount of a mineral is proportional to this . Since the antibacterial action depends on the amount of minerals , especially the amount of calcium dissolved, the antibacterial effect has been limited in the prior art. This is because, when minerals are dissolved, ionization of minerals other than calcium such as magnesium precedes ionization due to mutual interference between the ionization sequence derived from mineral components and the desired calcium in a solution under a certain amount of acetic acid. It was difficult to dissolve. Furthermore, the effects of chemical synthetic products and natural antibacterial agents are not necessarily effective against all microorganisms in food and the environment, and antibacterial agents that are particularly effective against fungi such as mold and spore-forming bacteria are rare. .
[0003]
[Problems to be solved by the invention]
The harmful fungi of fungi are also called filamentous fungi, and they multiply everywhere in the environment including the soil, causing a lot of damage to food, humans and houses. For example, mold poisons and mold production toxins cause diseases such as allergies, and foods, utensils, and houses have losses due to corruption and deterioration.
[0004]
The mold spores float in the air, adhere to the surface of the object, and the mycelium, fruiting bodies and spores form a flora colony, and further release spores and repeat their growth. Chemically synthesized products are widely used as antibacterial agents against fungi, but there is a possibility of health effects and environmental pollution, and there is a need for safer and more effective anti-fouling technologies.
[0005]
The spore-forming bacterium is a heat-resistant vegetative cell bacterium that forms a spore by environmental factors, survives in the form of a strong cell wall and low moisture spore, germinates by stimulation such as heat shock, and repeats the growth of the vegetative cell bacterium again. There are spores. The life-cycle of spore-forming bacteria is shown in FIG. (Refer to Tokyo Bunka Gakusha, New Food Hygiene.)
[0006]
Sporulation and illness occur in the process of spore-forming bacteria parasitically growing from soil to agricultural products, food, or feed to livestock. As representative spore-forming bacteria, Bacillus and Clostridium are known. In the spore state, since it shows strong resistance to heat, chemicals, ultraviolet rays, radiation, etc., there is a great need for a technique for preventing this growth. The most important cautions are hygiene, especially Bacillus subtilis, Bacillus cereus, Potulinus, and Bacillus subtilis, which produce food poisons.
[0007]
In order to heat sterilize spore-forming bacteria, Bacillus subtilis requires 3 hours at 100 ° C., which is difficult to implement in the processing of foods, and also wastes energy.
In order to achieve the above object, the present invention seeks a raw material for an antibacterial agent as a safe natural material, which is an organic material having a desired pH obtained by dissolving various minerals (inorganic salts) at a high concentration by a brewing acetic acid fermentation method. and e an acid mineral solution, and used to food, human, into the environment, fungi could not be easily realized with conventional, the growth inhibition of sporulation fungi, easily, and accurately prevent good living environment ( The purpose is to maintain safety and health .
[0008]
[Means for Solving the Problems]
The present invention has been made in order to solve the above-mentioned problems and achieve the above-mentioned object, and has a brewed acetic acid fermentation solution containing shells, eggshells, bone meals, coral shells, etc., having a pH of 6.0 or less, at least a high concentration ionic state, This is a method for producing an organic acid mineral solution antibacterial agent for inhibiting bacterial growth, wherein the calcium ion amount is dissolved by 3% or more.
[0009]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, one embodiment of the present invention will be described in detail.
First, the manufacturing method of a brewing fermentation acetate mineral solution is explained in full detail.
(1) Mineral (inorganic) raw materials;
Mineral (inorganic) raw materials use bone meal, eggshells, and shells, and these are heated and pulverized for sterilization and activation to be used alone or in combination (hereinafter, this mineral raw material is simply expressed as “mineral”) )
(2) fermentation;
Steam rice, cereals (wheat, corn), add alcohol and water. Acetic acid bacteria (Acenovacter, Gluconobacter) are added to this and fermented at room temperature.
(3) Dissolving mineral (inorganic) material in brewing fermentation acetic acid solution;
When fermentation proceeds and the pH of the solution becomes acidic, a portion of the mineral is added. When the fermentation progresses and the pH of the solution becomes acidic, a part of the mineral is added again. Further, the fermentation proceeds and the fermentation is stopped and the solution is taken out when the predetermined mineral is charged and the pH is 6 or lower. The brewing fermentation time is 36 to 72 hours.
The organic acid mineral solution thus produced is an antibacterial agent useful for inhibiting bacterial growth.
The component composition of the organic acid mineral solution produced by the above production method requires that the amount of calcium atoms in the solution be contained (dissolved) by cationic charge at least 3,000 mg% (weight ratio 3% ) or more. The content level above solution is than also you express new antimicrobial effect. The bone meal, eggshell, and shell used in the present invention are natural minerals, and the content ratio is also a natural balance.
[0010]
In the brewing process of brewing fermentation acetic acid solution, the raw alcohol is mainly acetic acid, and rice and shells are brewed as multiple organic acids such as acetic acid, tartaric acid, citric acid, succinic acid, malic acid, gluconic acid and fumaric acid. Is done. Mineral components are composed of calcium, sodium, potassium, magnesium, phosphorus, iron, zinc and the like. Organic acids undergo chemical changes with mineral components in solution to form cation metal salts and anion metal salts. There are calcium, sodium, potassium, and iron zinc as cation metal salts, which raise the pH . Phosphorus as a non-metal salt of the anion, sulfur, there are iodine, lowering the pH. As the total amount of minerals, there are many cations and the pH rises. By further promoting fermentation, an organic acid is generated, and after the reaction with the minerals in the solution, the fermentation is further stopped when the pH is 6 or 6 or less.
[0011]
Next, embodiments of the present invention will be described by way of examples.
[Example 1]
65% shell configuration of mineral material, 30% eggshell, and 5% coral acid, dissolving the minerals acetate solution in brewing fermentation, stop fermentation at pH 4.8, was prepared organic acid mineral solution A. This is A agent.
[ 0012 ]
[Example 2]
The mineral material composition was 75% eggshell and 25% shell, and this mineral was dissolved in an acetic acid solution during brewing and fermentation was stopped at pH 4.8 to produce an organic acid mineral solution B. This is B agent.
[ 0013 ]
[Verification Example 1]
About heat-resistant spore bacteria, A agent was used and the antimicrobial effect was tested.
Select more crops Azuki heat spore-forming bacteria Bacillus derived from soil as a spore-forming bacteria and subjected Antoshi, bacterial testing braised heating it for about 120 minutes.
(1) those added A agent 0.5% adzuki bean jam (2) without the addition of A agent to red bean sauce (no additives) those for 3 days, and hot storage at 20 degrees Celsius, the bacterial growth after 3 days The state was observed and the results are shown in Table 1.
[ 0014 ]
[Table 1]

[ 0015 ]
As is apparent from Table 1 , heat-resistant spore bacteria (Bacillus subtilis) were actively growing in the additive-free group, and this bacterium was Bacillus subtilis .
In addition, the number of general viable bacteria also increased in the non-addition zone, and the state was just before rot .
The growth of Bacillus subtilis in addition group of A agent was not observed.
[ 0016 ]
[Verification Example 2]
Next, the spore formation of the fungus grown in Verification Example 1 was confirmed, a spore was collected from the medium to prepare a suspension, and heated at 100 ° C. for 5 minutes to give a heat shock to the germination of the spore. The suspension was inoculated on a culture plate containing the agent A diluted to the concentration shown in Table 2, and the effect of the agent A inhibiting the growth of heat-resistant spore bacteria was tested.
In addition, a suspension inoculation medium that was not reheated was prepared, and a growth test for spores and vegetative cells was also conducted. The results are shown in Table 2.
[ 0017 ]
[Table 2]

[ 0018 ]
As is apparent from Table 2, the bacteria grew when the agent A was not mixed (target 0%), but the growth was not observed on the plate mixed with the agent A 0.8% or more. In addition, since the reheated spore mold plate is sterilized by heating, the medium is inoculated with only the spore mold. On the other hand, in the plate of the non-reheat bacteria, fungi colonies as the total amount for viable microorganisms coexisting has not been autoclaved was observed slightly more tendency.
[ 0019 ]
From the results in Table 2, the characteristics of the spore germination of heat-resistant S. spore bacteria are that heat shock gives the spore germination, which leads to strong reincarnation and cell growth from germination to vegetative cells. , cell membranes of mineral ions by high osmotic pressure of the a agent, the permeability of the cell wall, minerals modified cellular substrates, a great influence on bacterial growth environment, such as protein synthesis enzyme inactivation, given a heat shock, germination prompting It was demonstrated that the growth of the heat-resistant spore bacteria was suppressed.
As described above, the growth inhibition of Bacillus subtilis, a heat-resistant spore-forming bacterium, has conventionally involved a complicated process and a large amount of energy consumption. However, the organic acid mineral solution according to the present invention has a constant concentration (0.8% or more). It was confirmed that it was easily and accurately stopped.
Use in the production, distribution and storage of food as an antibacterial agent of the organic acid mineral solution of the present invention is beneficial to health and hygiene, improves economic efficiency, and saves energy.
[ 0020 ]
[Verification Example 3]
A myriad of fungal spores flying in the air grow on the object and propagate to form colonies as a flora. Therefore, the effect of inhibiting the growth after spore establishment was tested. Mochi is a food that is low in water and prone to mold generation, so it is possible to easily carry out tests for confirming mold growth and growth.
For the test, the following types of test products were prepared for the same production conditions .
(1) No processing
(2) A solution obtained by diluting B agent 10 times with water is applied to one side of the rice cake with a brush.
These were stored in the same container where air can freely enter and exit and left for 21 days , and the state in the process was observed. The results are shown in Table 3.
[ 0021 ]
[Table 3]

[ 0022 ]
The mold grown on the untreated product was the fungus Penicillium blue mold.
This test is carried out by storing the test product in the same container where air can freely enter and exit, and mold spores can be confirmed to adhere to each test product. It penetrates the cell wall of the spore, denatures the cell matrix, inactivates protein synthase, inhibits assimilation, inhibits the growth of carbohydrates as organic organic fungi of fungi, and prevents the intake of nutrients from nitrogen sources. I was able to confirm that it was blocking.
Such effects, food or dilute the safety of high antibacterial agent, was applied directly to the vessels, but a long time to maintain the effect, and shows an excellent effect of the present invention the organic acid mineral solution .
[ 0023 ]
[Verification Example 4]
Shantou is a food that uses rice flour, yam, and sugar as ingredients, and has a high water content, and the growth of bacteria, fungi, and spoilage bacteria derived from the raw materials is fast. Agent A was applied as follows and steamed for 12 minutes as usual to prepare a test product . In addition, an untreated product to which agent A was not added was prepared under the same conditions, and a fungus growth inhibition test was conducted.
(1) No treatment (2) Dilute agent A 5 times with water, spray on the outside of the wharf and steam
The test article was stored indoors at room temperature, and a comparative test of mold generation and growth inhibition was performed. The results are shown in Table 4.
[ 0024 ]
[Table 4]

[ 0025 ]
Untreated molds are seen as Aspergillus and Eurotium, which normally grow on processed starch products.
In a comparative test, the same amount of spore formation was observed under the same storage conditions, but it was demonstrated that the antibacterial agent inhibited the germination and growth of fungi in the test product to which agent A was added. Yes. In addition, about the effect | action of A agent, it is the same as the description of the verification example 3. FIG.
As apparent from the above simulation example, A agent without also reducing its efficacy is heated, there is no impact on the temperature change during processing, there are stable effect can be safely and easily used, The effect is accurate and lasts for a long time.
[ 0026 ]
[Verification Example 5]
Yeast Ru is used primarily as useful bacteria, but also be a sometimes harmful fungi. Useful bacteria include the yeast growth, that is, the operation of fermentation, and harmful bacteria include the expansion of packaged food due to the generation of carbon dioxide by yeast growth, bag breakage, and septic fermentation . An organic acid mineral solution is used as an antibacterial agent for this harmful effect.
The B agent is added to bread dough that acts as a baker's yeast and a comparative test is performed on the change in expansion rate. The results are shown in Table 5.
[ 0027 ]
[Table 5]

[ 0028 ]
Yeast is a single-cell microorganism larger than bacteria and fungi, and its cell wall composition is strong such as glucan and mannan, so it is generally said that it is difficult to use antibacterial agents. The amount of antibacterial agents added to bacteria and fungi An amount greater than the amount used was required. For such situation, by the action of an organic acid mineral solution hypertonic, cell wall, to penetrate the cell membrane, it affects and inhibitory effect on the plasma cell contents, prevent the yeast fermentation, delayed effect Has been demonstrated . This as a technology utilizing yeast, or antimicrobial agent to excellent effect as corruption growth inhibition by yeast contamination, is effective.
[ 0029 ]
[Verification Example 6]
High osmotic pressure of minerals such as calcium to vegetative cell bacteria increases free water due to exudation of cell-bound water, protein denaturation, and high osmotic pressure for vegetative cells under the proliferation cycle of cell division In order to see the antibacterial effect in the relationship between bacterial growth and water separation by inhibiting action, inhibition of nutrient intake, that is, inhibition of assimilation, custard cream that is subject to spoilage in a short time was used as a verification target, and this increased the number of general viable bacteria The antibacterial effect due to water separation was tested on custard cream to which agent A was added. The results are shown in Table 6.
[ 0030 ]
[Table 6]

[0031]
In the non-addition zone, the growth of bacteria and water separation proceeded in proportion , and in the addition zone, the inhibition of growth worked effectively, and in the addition zone, it was confirmed that there was no water separation.
As verified in the above-described verification examples, the organic acid mineral solution has a sufficient effect as an antibacterial agent even in an optimal growth environment such as spore-forming bacteria, molds, and yeast . This is of course more effective for bacteria that are less resistant than these. It was simply further can confirm the effect by looking at the growth inhibition in both sides of the relationship between corruption and water separation.
[ 0032 ]
[Verification Example 7]
Black mold is said to be a causative agent of asthma and allergies, and is a fungus that requires countermeasures in environmental sanitation. Tests were conducted on black molds on bathroom tile joints.
The agent B was mixed and diluted with local petrolatum at a ratio of 3% (hereinafter referred to as petrolatum with an antibacterial agent) and used for the test.
A test area was set in the bathroom tile joint where black mold was densely grown in advance , and after washing with a detergent, a test of black mold spore implantation and proliferation was performed. Black mold around the test area remains the same.
(1) Test area A: Remove mold with detergent and apply petrolatum only (2) Test area B: Apply fungus with antibacterial agent after removing mold with detergent Antibacterial effect by measuring the time difference until the occurrence of new mold Was judged . The results are shown in Table 7.
[ 0033 ]
[Table 7]

[ 0034 ]
In the test group A , black mold colonies grew after 25 days and grew to the same density as the surroundings after 37 days . On the other hand, in the test area B, black mold colonies became the same as the surroundings 78 days after the occurrence of the colonies.
Black mold (Cladosporium earth), in addition to vessels of residential, agricultural products, such as the breeding is extensive to contact good department. Not only antibacterial activity related food, further widening the subject, even in different residential environments conditions food, organic acid mineral solution are the same antibacterial effect. Even under conditions suitable for the growth of black mold, it has direct spore penetration, degeneration of cell substrate, germination of spore, and inhibition of growth by inhibiting growth, and it is safe and accurate antibacterial in a wide range of living environments Has an effect.
[ 0035 ]
[Verification Example 8]
Filamentous fungi that infect human skin are called dermatophytes, and there are three genera: oligospores, ringworms, and epidermis. Representative ringworms grow on the skin and cause infections such as shirakamo, worms, and beetles. An antibacterial test of an organic acid mineral solution against this ringworm was performed. The exam
Inoculated culture with tinea fungus in an additive-free medium,
Inoculated culture with ringworm on the supplemented medium diluted with 0.5% of agent A,
Inoculated culture with ringworms in supplemented medium with A agent diluted to 1.0%
These three types of medium were used to observe the growth and inhibition of ringworm fungus. The results are shown in Table 8.
[ 0036 ]
[Table 8]

[ 0037 ]
Since dermatophytes have conditions for growth such as nutrients derived from humans and conditions such as temperature, the effects of culture and growth inhibition were compared in mediums of nutrients suitable for them. As a result, it can be confirmed that the organic acid mineral solution is effective in inhibiting cell growth, and it exhibits excellent efficacy as an innocuous and antibacterial agent for infectious diseases that live near the human epidermis. I was able to confirm .
[ 0038 ]
[Verification Example 9]
In general, when the pH is 4.5 or less, the growth of bacteria is suppressed, but this is because the cellular substrate is affected by minerals that permeate the cell membrane due to strong osmotic pressure at low pH of intracellular proteins of microorganisms, And it inhibits the growth function of bacteria, and as a result acts as a blocking. This action is due to the fact that organic acids partially dissociate in solution, but as the hydrogen ion concentration increases, the dissociation rate decreases and non-dissociated molecules permeate the bacterial cell membrane to cause protein denaturation.
[ 0039 ]
The pH of a 0.5% aqueous solution of citric acid is 2.8, the addition of 0.5% citric acid aqueous solution was added to agent A 1%, there is no decrease in pH was pH 4.8. This brings about the advantage that the action of high osmotic pressure or the action of inhibiting the growth of bacteria, which has been realized only at a low pH of 4.5 or less , is not limited to a strongly acidic region. In other words, organic acid mineral solutions improve the effect on taste (sourness) in foods, alleviate excessive acid irritation in human skin, and oxidize and corrode equipment in the environment. This is also the reason why it has sufficient osmotic pressure to penetrate the bacterial cell membrane and cell wall.
[ 0040 ]
Table 9 shows changes in the dilution ratio and pH of an aqueous solution obtained by diluting an organic acid mineral solution prepared by the production method of the present invention with water. As is apparent from Table 9, the aqueous solution obtained by diluting the organic acid mineral solution has the ability to maintain the pH even if the hydrogen ion concentration in the aqueous solution is in the condition of increasing the pH while maintaining weak acidity. Can be confirmed. Thus, the organic acid mineral solution has a buffering action over a wide range, and has excellent characteristics not found in conventional antibacterial agents.
[ 0041 ]
[Table 9]

[ 0042 ]
Since the organic acid mineral solution of the present invention is manufactured using raw materials used for food as raw materials, it can provide an antibacterial agent that is safe and can be used in a wide range of applications . The organic acid mineral solution prepared in the present invention maintains an osmotic pressure far higher than the conventional osmotic pressure under a pH range lower than the conventional one even if it is not lowered to pH 4.5 or lower, and the cell walls and cell membranes of bacteria are maintained. Has antibacterial effect to penetrate.
The high mineral ion concentration affects the cell substrate and inhibits cell growth factors, thereby preventing the growth of bacteria.
In addition, inhibition of bacterial assimilation has appeared in the effect of blocking the germination of mold spores over a long period of time. In food, it prevents spoilage without affecting the taste, saves energy, and reduces the harmful effects of antibacterial resistance due to heavy use of antibiotics and habituation of antibiotics.
[ 0043 ]
The present invention provides an organic acid mineral solution that has a novel effect even in a living environment and that effectively acts on environmental sanitation.
It also has the effect of improving human health and preventing resource loss. When this organic acid mineral solution is used as an antibacterial agent for human skin infection fungi, it becomes an antibacterial agent that is not particularly irritating and is safe for absorption in the body.
Since the present organic acid mineral solution has a wide range of buffering action and has a buffering function, it can be used as a diluent for many kinds of drugs. In weakly acidic region, it is effective as a new diluent such can exhibit drug effect of strongly acidic range.
[ 0044 ]
【The invention's effect】
The present invention seeks a safe natural material as a raw material for an antibacterial agent, and dissolves various minerals (inorganic salts) at a high concentration by a brewing acetic acid fermentation method to produce an organic acid mineral solution having a desired pH. It has an excellent effect that can be achieved.
In addition, this organic acid mineral solution is used as an antibacterial agent in food, humans, and the environment, and it can easily and accurately prevent the growth of fungi and spore-forming fungi that have not been easily realized in the past. It has excellent effects that can maintain the environment (safety and health).
[Brief description of the drawings]
FIG. 1 is an explanatory diagram of the life cycle of spore-forming bacteria.

Claims (1)

  Organic acid for inhibiting bacterial growth, characterized in that shellfish, eggshell, bone meal, coral shell, etc. are dissolved in a brewed acetic acid fermentation solution at a pH of 6.0 or less and at a high concentration, at least 3% or more calcium ions. Manufacturing method of mineral solution antibacterial agent.

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