JP3715151B2 - Method of distinguishing moisturizing ingredients and cosmetics - Google Patents
Method of distinguishing moisturizing ingredients and cosmetics Download PDFInfo
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- JP3715151B2 JP3715151B2 JP29606899A JP29606899A JP3715151B2 JP 3715151 B2 JP3715151 B2 JP 3715151B2 JP 29606899 A JP29606899 A JP 29606899A JP 29606899 A JP29606899 A JP 29606899A JP 3715151 B2 JP3715151 B2 JP 3715151B2
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- 239000004615 ingredient Substances 0.000 title description 9
- 239000000203 mixture Substances 0.000 claims description 42
- 210000002510 keratinocyte Anatomy 0.000 claims description 32
- 210000003491 skin Anatomy 0.000 claims description 32
- 238000007654 immersion Methods 0.000 claims description 27
- 238000002360 preparation method Methods 0.000 claims description 10
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- 239000006210 lotion Substances 0.000 description 6
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 6
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- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 4
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- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
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- WCVRQHFDJLLWFE-UHFFFAOYSA-N pentane-1,2-diol Chemical compound CCCC(O)CO WCVRQHFDJLLWFE-UHFFFAOYSA-N 0.000 description 1
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Cosmetics (AREA)
Description
【０００１】
【発明の属する技術分野】
本発明は、化粧料などの保湿成分を含む皮膚外用の組成物の保湿作用の鑑別法及び、該鑑別法により優れた効果を有すると鑑別された保湿成分を含む皮膚外用の組成物に関する。
【０００２】
【従来の技術】
保湿作用は、化粧料や皮膚外用医薬などの皮膚外用剤の製剤設計に於いては、重要な概念であり、この作用の特質の如何によっては、同じ有効成分を含んでいても、その外用剤の効果は大きく異なってしまう。この為、製剤成分の保湿作用や製剤全体の保湿作用を鑑別することは重要な技術となっている。しかるに、現在行われている保湿作用を鑑別する手だてとしては、散逸水分量を測る手段や皮膚のコンダクタンスなど水分に起因する物理的数値を測定する手段と、肉眼的所見観察と使用実感を被験者よりアンケートで回答してもらう等が挙げられるが、これらは何れも二次的な測定値や主観的な評価であることは否めなかった。更に、詳細に言えば、これらの測定の内物理的数値については、皮膚内に存在する水分の指標にはなっても、生理的な保水性指標とは言い難かった。即ち、生理的な意義を加味した保湿作用の鑑別法の開発が望まれていた。
【０００３】
一方、角質細胞の厚さ乃至は体積を指標に保湿作用を鑑別することは全く為されていなかったし、この鑑別結果を元に化粧料の個対応の選択を行うことも全く為されていなかった。
【０００４】
【発明が解決しようとする課題】
本発明は、この様な状況下為されたものであり、生理的な意義を加味した保湿作用の鑑別法を提供し、個対応された保湿成分を含有する皮膚外用組成物を提供することを課題とする。
【０００５】
【課題の解決手段】
この様な状況に鑑みて、本発明者らは生理的な意義を加味した保湿作用の鑑別法を求めて鋭意研究努力を重ねた結果、角質細胞を保湿成分を含む皮膚外用の組成物に浸漬し、浸漬前後の角質細胞の厚さ及び／又は体積を測定し、該厚さ及び／又は体積の変化を指標とすることにより、この様な鑑別が為し得ることを見出した。更に研究を重ねた結果、この様な鑑別法において、人の頬部より採取した角質細胞を該保湿成分を含む皮膚外用の組成物に１２時間浸漬し、浸漬前と浸漬より取り出して３時間後の角質細胞の厚さ及び／又は体積を測定し、浸漬終了後３時間の該厚さ及び／又は体積が浸漬前の該厚さ及び／又は体積に比して２０％以上の増大であることを特徴とするものが、生理的な保湿作用において優れた皮膚外用剤であることを見出し発明を完成させるに至った。即ち、本発明は、角質細胞を保湿成分を含む皮膚外用の組成物に浸漬し、浸漬前後の角質細胞の厚さ及び／又は体積を測定し、該厚さ及び／又は体積の変化を指標とする保湿作用の鑑別法を提供するものである。
【０００６】
更に本発明は、この様な鑑別法において、人の頬部より採取した角質細胞を該保湿成分を含む皮膚外用の組成物に１２時間浸漬し、浸漬前と浸漬より取り出して３時間後の角質細胞の厚さ及び／又は体積を測定し、浸漬終了後３時間の該厚さ及び／又は体積が浸漬前の該厚さ及び／又は体積に比して２０％以上の増大する事を特徴とする皮膚外用剤を提供するものである。
【０００７】
【発明の実施の形態】
（１） 本発明の保湿作用の鑑別法
本発明の保湿成分を含有する皮膚外用の組成物の保湿作用の鑑別法は、角質細胞を保湿成分を含む皮膚外用の組成物に浸漬し、浸漬前後の角質細胞の厚さ及び／又は体積を測定し、該厚さ及び／又は体積の変化を指標とすることを特徴とする。ここで、保湿成分とは皮膚に必要な水分を生理的に保持する機能を有する成分の総称を意味する。かかる保湿成分としては、例えば、プロピレングリコール、１，３−ブタンジオール、１，２−ペンタンジオール、イソプレングリコール、グリセリン、ジグリセリンなどのポリグリセリン、ジプロピレングリコールなどのポリプロピレングリコール、ポリエチレングリコール、ソルビトール、マルチトール等の糖類等の多価アルコール、硫酸化多糖類、多硫酸化多糖類、硫酸化糖類、アシル化糖類などの糖誘導体、ホスファチジルコリン、ポリメタクリロイルオキシエチルコリン等の燐脂質とその誘導体等が例示できる。又、この様な保湿成分を含有する皮膚外用の組成物としては、化粧料、皮膚外用医薬、外用殺菌消毒剤などが好ましく例示でき、この中でも化粧料と皮膚外用医薬がより好ましく例示でき、化粧料が特に好ましい。本発明の鑑別法に於いては、かかる保湿成分を含有する組成物中に動物より採取した角質細胞を浸漬し、浸漬前と浸漬後の細胞の厚さ及び／又は体積を測定しその変化率を求めて指標とする。ここで角質細胞を採取する動物としては、マウス、ラット、モルモット、ウサギ、ブタなどの実験動物や人が例示できる。採取方法は、実験動物であれば背部の毛を剃毛した後、人であればそのまま粘着テープなどによりストリッピングして採取する方法が好ましく例示できる。培養皮膚を培養して得られた角質細胞を使用することも可能である。この場合、細胞が安定しているため、組成物の比較には個体差を排除することができる。又、動物を人にした場合、組成物の影響の仕方やその程度に肌性などの個体差があるため、これを利用してその角質細胞の持ち主に適した保湿成分を含有する皮膚外用の組成物を選択する指標として用いることもできる。この様な技術も本発明の技術的範囲に属する。更に、組成物を固定して、角質細胞を変えて、角質細胞の変化の仕方を元に肌性などの角質細胞或いはそれに起因する肌質などの肌の特性を鑑別する技術も本発明の技術的範囲に属する。浸漬は組成物が大過剰になるように行うのが好ましい。かかる技術において、角質細胞の厚さ乃至は体積を測定する手段としては，原子間力顕微鏡を用いて、その像を画像解析することが好ましく例示できる。角質細胞の皮膚外用の組成物中への浸漬時間は平衡に達する程度の時間が好ましく、少なくとも１０時間、より好ましくは１２時間が例示できる。この浸漬の後に取り出して３時間程度まで経時的に厚さ乃至は体積を測定すると更に保湿に対する作用を詳しく鑑別することができる。
【０００８】
本発明の鑑別法において、有効な保湿作用を有すると判定されるためには、上記の手技に従って角質細胞の厚さ及び／又は体積を測定し、その変化率が２０％以上であることが条件となる。
【０００９】
（２） 本発明の皮膚外用剤
本発明の皮膚外用剤は上記鑑別法によって有効と鑑別されていることを特徴とする。即ち、保湿成分を含む皮膚外用の組成物において、人の頬部より採取した角質細胞を該保湿成分を含む皮膚外用の組成物に１２時間浸漬し、浸漬前と浸漬より取り出して３時間後の角質細胞の厚さ及び／又は体積を測定し、浸漬終了後３時間の該厚さ及び／又は体積が浸漬前の該厚さ及び／又は体積に比して２０％以上の増大であることを特徴とする。この様な化粧料は、優れた保湿作用を発揮し、角層のバリア機能を高める作用を有するため、この様な機能の衰えている敏感肌の人やアトピー性皮膚炎の人などに非常に好適である。
【００１０】
【実施例】
以下に、実施例を挙げて本発明について更に詳細に説明を加えるが、本発明がかかる発明にのみ限定を受けないことは言うまでもない。
【００１１】
＜実施例１＞
表１に示す保湿成分を含有する皮膚外用組成物（化粧水）を作成し、敏感肌の人より採取した角質細胞と普通肌の人より採取した角質細胞を１２時間浸漬し、浸漬終了後３時間にその厚さ、面積及び体積を、原子間力顕微鏡及び画像解析を用いて計測した。計測の代表値は２０個の計測の平均値とした。結果を表１に示す。これより、保湿成分の種類により厚さと体積は変化していることがわかる。しかしながら、面積の変化は殆どなく、化粧料の適性は角質細胞の面積よりも厚さ或いは体積に大きく関与していることが推察される。尚、単位は厚みがμｍ、面積がμｍ 2 、体積がμｍ 3 であった。これより、保湿成分の種類により、角質細胞の厚さ及び／又は体積に対する影響が異なることがわかる。これらの化粧水の内、本発明の皮膚外用の組成物に該当するのは保湿剤をグリセリンに選択したもののみであった。（変化率＋２３％）
化粧料の処方
保湿成分＊ ５ 重量部
エタノール ５ 重量部
メチルパラベン ０．２重量部
水 ８９．８重量部
＊表１に詳細を記す。
【００１２】
【表１】
＜実施例２＞
下記に示す処方に従って化粧水を製造した。即ち、処方成分を攪拌し、可溶化して化粧水を得た。このものに敏感肌の人より角質細胞を採取し、１２時間組成物中に浸漬し、取り出した後、経時的に計測しながら３時間観察を続けた。浸漬前は厚さが０．１４１μｍ、面積が１６４６μｍ 2 、体積が２２８μｍ 3 であり、浸漬終了後１時間の厚さが０．１８９μｍ、面積が１４２６μｍ 2 、体積が２６４μｍ 3 であり、２時間後の厚さが０．１９６μｍ、面積が１４２３μｍ 2 、体積が２７３μｍ 3 であり、３時間後の厚さが０．２００μｍ、面積が１３８３μｍ 2 、体積が２７７μｍ 3 であり、このものは本発明の皮膚外用の組成物であることが判明した。又、体積と厚さは増大しているが面積は変化していないこともわかり、皮膚機能との関係は面積よりも体積や厚みの方がより密接であることもわかる。加えて、本発明の化粧料に於いては、浸漬終了後放置しても細胞内の水分を放出していないこともわかる。このものを角質細胞を採取した人に１ヶ月間使用してもらったところ、炎症などの不都合な現象は全く発生せず、専門家による肉眼観察結果では使用前に比し、使用後に肌状態が有意に改善していた。これより、本発明の鑑別法が使用化粧料の選択に好適な示唆を与えることがわかる。
１，３−ブタンジオール ８ 重量部
グリセリン ９ 重量部
ポリエーテル変性シリコーン １ 重量部
ポリオキシエチレン硬化ヒマシ油 ０．３重量部
メチルパラベン ０．２重量部
水 ８１．５重量部
【００１４】
＜実施例３＞
下記に示す処方に従って化粧水を製造した。即ち、処方成分を攪拌し、可溶化して化粧水を得た。このものに敏感肌の人より角質細胞を採取し、１２時間組成物中に浸漬し、取り出した後、経時的に計測しながら３時間観察を続けた。浸漬前は厚さが０．１７５μｍ、面積が１４５８μｍ 2 、体積が２４１μｍ 3 であり、浸漬終了後１時間後の厚さが０．１４１μｍ、面積が１４３９μｍ 2 、体積が２００μｍ 3 であり、２時間後の厚さが０．１６７μｍ、体積が２４２μｍ 3 であり、３時間後の厚さが０．１６８μｍ、面積が１４３９μｍ 2 、体積が２３９μｍ 3 であり、体積、厚さ共に変化は見られず、このものは本発明の皮膚外用の組成物でないことが判明した。又、この様な組成物に於いては、浸漬終了後角質細胞の厚さ乃至は体積が減少していることもわかる。これは、内部に吸収した水分が放出されるためである。このものを角質細胞を採取した人に使用してもらったところ、１週間後にブツを発生した為、使用を中止した。これより本発明の鑑別法のこの化粧料はこのパネラーに適しているとはいえないとの鑑別結果が正しかったことが分かる。
ポリエチレングリコール １ 重量部
グリセリン ３ 重量部
イソプレングリコール ２ 重量部
メチルパラベン ０．１重量部
水 ９３．９重量部
【００１５】
【発明の効果】
本発明によれば、生理的な意義を加味した保湿作用の鑑別法を提供し、個対応された保湿成分を含有する皮膚外用組成物を提供することができる。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a method for distinguishing a moisturizing action of a composition for external use containing a moisturizing component such as a cosmetic, and a composition for external use containing a moisturizing component identified as having an excellent effect by the distinguishing method.
[0002]
[Prior art]
Moisturizing action is an important concept in the formulation design of external preparations for skin, such as cosmetics and external preparations for skin. Depending on the nature of this action, the external preparation may contain the same active ingredient. The effect of is very different. For this reason, it is an important technique to distinguish the moisturizing action of the formulation components and the moisturizing action of the entire formulation. However, as a means of distinguishing the current moisturizing effect, there are means for measuring the amount of dissipated water, means for measuring physical numerical values due to moisture such as skin conductance, macroscopic observation and actual use feeling from the subject. There is an answer to the questionnaire, etc., but none of these were secondary measurements or subjective evaluations. More specifically, even though these physical values of these measurements are indicators of moisture present in the skin, they are difficult to say as physiological water retention indicators. That is, it has been desired to develop a method for distinguishing moisturizing action in consideration of physiological significance.
[0003]
On the other hand, the moisturizing effect was not discriminated at all by using the thickness or volume of the keratinocytes as an index, and the individual selection of cosmetics was not made at all based on this discrimination result. It was.
[0004]
[Problems to be solved by the invention]
The present invention has been made under such circumstances, and provides a method for distinguishing moisturizing action that takes physiological significance into consideration, and provides an external composition for skin containing a moisturizing component corresponding to the individual. Let it be an issue.
[0005]
[Means for solving problems]
In view of such a situation, the present inventors have sought to identify a moisturizing action that takes physiological significance into consideration, and as a result of intensive research efforts, dipped keratinocytes in a composition for external use containing a moisturizing component. Then, the thickness and / or volume of the stratum corneum before and after immersion was measured, and it was found that such a differentiation could be made by using the change in the thickness and / or volume as an index. Further studies, in such a differentiation method, was immersed for 12 hours horny cells collected from the cheek of the person in the composition of the skin external containing-holding wet ingredients, 3 hours after taken out of the immersion and before immersion The thickness and / or volume of keratinocytes is measured, and the thickness and / or volume for 3 hours after the immersion is increased by 20% or more compared to the thickness and / or volume before the immersion. Was found to be an external preparation for skin excellent in physiological moisturizing action, and the present invention was completed. That is, in the present invention, keratinocytes are immersed in a composition for external use containing a moisturizing component, the thickness and / or volume of the keratinocytes before and after immersion are measured, and the change in the thickness and / or volume is used as an index. It provides a method for distinguishing the moisturizing effect.
[0006]
Further, according to the present invention, in such a discrimination method, keratinocytes collected from a human cheek are soaked in a composition for external use containing the moisturizing component for 12 hours, and the keratin after 3 hours after taking out from the soaking and after soaking. The thickness and / or volume of a cell is measured, and the thickness and / or volume after 3 hours of immersion is increased by 20% or more compared to the thickness and / or volume before immersion. An external preparation for skin is provided.
[0007]
DETAILED DESCRIPTION OF THE INVENTION
(1) Method of distinguishing moisturizing action of the present invention The method of distinguishing the moisturizing action of the composition for external use of the skin containing the moisturizing ingredient of the present invention comprises immersing the keratinocytes in the composition for external use of the skin containing the moisturizing ingredient. The thickness and / or volume of keratinocytes is measured, and the change in the thickness and / or volume is used as an index. Here, the moisturizing component means a general term for components having a function of physiologically holding water necessary for the skin. Examples of the moisturizing component include propylene glycol, 1,3-butanediol, 1,2-pentanediol, isoprene glycol, polyglycerol such as glycerol and diglycerol, polypropylene glycol such as dipropylene glycol, polyethylene glycol, sorbitol, Polysaccharides such as saccharides such as maltitol, saccharides such as sulfated polysaccharides, polysulfated polysaccharides, sulfated saccharides, acylated saccharides, phospholipids such as phosphatidylcholine and polymethacryloyloxyethylcholine and their derivatives, etc. It can be illustrated. Further, examples of the composition for external use containing such a moisturizing ingredient include cosmetics, external preparations for skin, and antibacterial disinfectants for external use. Among these, cosmetics and external preparations for skin can be more preferably exemplified. The material is particularly preferred. In the differentiation method of the present invention, keratinocytes collected from an animal are immersed in a composition containing such a moisturizing component, and the thickness and / or volume of the cells before and after immersion are measured, and the rate of change is measured. Is used as an index. Examples of animals from which corneocytes are collected include laboratory animals such as mice, rats, guinea pigs, rabbits, and pigs, and humans. As a sampling method, a method of shaving the back hair of a laboratory animal and then stripping it with an adhesive tape as it is for a human can be preferably exemplified. It is also possible to use keratinocytes obtained by culturing cultured skin. In this case, since the cells are stable, individual differences can be excluded in the composition comparison. In addition, when an animal is a human, there are individual differences such as skin properties in the manner and extent of the effect of the composition. Therefore, using this, it is intended for external use that contains a moisturizing component suitable for the owner of the keratinocytes. It can also be used as an index for selecting a composition. Such a technique also belongs to the technical scope of the present invention. Furthermore, the technique of fixing the composition, changing the keratinocytes, and distinguishing the skin characteristics such as keratinocytes such as skin properties or the skin quality resulting from the keratinocytes based on the change of the keratinocytes is also a technique of the present invention. Belong to the scope. Immersion is preferably performed so that the composition is in a large excess. In such a technique, as a means for measuring the thickness or volume of the keratinocytes, it is preferable to analyze an image of the image using an atomic force microscope. The soaking time of the corneocytes in the composition for external use on the skin is preferably a time to reach equilibrium, and can be exemplified by at least 10 hours, more preferably 12 hours. Taking out after this immersion and measuring the thickness or volume over time up to about 3 hours can further distinguish the action on moisture retention.
[0008]
In the discrimination method of the present invention, in order to be determined to have an effective moisturizing action, the thickness and / or volume of keratinocytes is measured according to the above procedure, and the rate of change is 20% or more. It becomes.
[0009]
(2) External preparation for skin of the present invention The external preparation for skin of the present invention is characterized by being identified as effective by the above-described discrimination method. That is, in the composition of the skin external including moisturizing, for 12 hours in the compositions of the skin external including keratinocytes and-holding wet components collected from the cheek of a human, after 3 hours was removed from the immersion and before immersion The thickness and / or volume of the keratinocytes is measured, and the thickness and / or volume after 3 hours of immersion is increased by 20% or more compared to the thickness and / or volume before immersion. Features. Such cosmetics have an excellent moisturizing effect and enhance the barrier function of the stratum corneum, so it is very suitable for people with sensitive skin and those with atopic dermatitis who have such functions declined. Is preferred.
[0010]
【Example】
Hereinafter, further is added described in detail about the present invention by way of example, it is needless to say not subjected to limited the present invention is applied invention.
[0011]
<Example 1>
A skin external composition (lotion) containing the moisturizing ingredients shown in Table 1 was prepared, and keratinocytes collected from a person with sensitive skin and keratinocytes collected from a person with normal skin were immersed for 12 hours. Over time, its thickness, area and volume were measured using atomic force microscopy and image analysis. The representative value of the measurement was an average value of 20 measurements. The results are shown in Table 1. From this, it can be seen that the thickness and volume change depending on the type of the moisturizing component. However, there is almost no change in the area, and it is assumed that the suitability of the cosmetic is more largely related to the thickness or volume than the area of the corneocytes. The unit was μm in thickness, area in μm 2 , and volume in μm 3 . From this, it can be seen that the effect on the thickness and / or volume of keratinocytes varies depending on the type of moisturizing component. Of these lotions, only the ones selected as the humectant as the humectant corresponded to the composition for external use of the skin of the present invention. (Change rate + 23%)
Prescription moisturizing ingredients for cosmetics * 5 parts by weight ethanol 5 parts by weight methyl paraben 0.2 parts by weight water 89.8 parts by weight * Details are given in Table 1.
[0012]
[Table 1]
<Example 2>
A lotion was produced according to the formulation shown below. That is, the prescription ingredients were stirred and solubilized to obtain a lotion. The corneocytes were collected from a person with sensitive skin, immersed in the composition for 12 hours, taken out, and then observed for 3 hours while measuring over time. Before immersion, the thickness is 0.141 μm , the area is 1646 μm 2 , and the volume is 228 μm 3. After the immersion, the thickness is 0.189 μm , the area is 1426 μm 2 , and the volume is 264 μm 3 . The thickness after 2 hours is 0.196 μm , the area is 1423 μm 2 , the volume is 273 μm 3 , the thickness after 3 hours is 0.200 μm , the area is 1383 μm 2 , and the volume is 277 μm 3 This was found to be the composition for external use of the skin of the present invention. It can also be seen that the volume and thickness have increased, but the area has not changed, and that the relationship with the skin function is that the volume and thickness are closer than the area. In addition, it can be seen that in the cosmetic of the present invention, intracellular moisture is not released even if it is allowed to stand after immersion. When this sample was used by a person who collected corneocytes for 1 month, there was no inconvenience such as inflammation at all, and the result of naked eye observation by an expert showed that the skin condition after use was higher than before use. There was a significant improvement. From this, it can be seen that the discrimination method of the present invention provides a suitable suggestion for the selection of the cosmetics to be used.
81.5 parts by weight of 1,3-butane diol 8 parts by weight of glycerin 9 parts by weight polyether-modified silicone 1 part by weight polyoxyethylene hydrogenated castor oil 0.3 parts by weight Methylparaben 0.2 parts by weight of water [0014]
<Example 3>
A lotion was produced according to the formulation shown below. That is, the prescription ingredients were stirred and solubilized to obtain a lotion. The corneocytes were collected from a person with sensitive skin, immersed in the composition for 12 hours, taken out, and then observed for 3 hours while measuring over time. Before immersion, the thickness is 0.175 μm , the area is 1458 μm 2 , the volume is 241 μm 3 , the thickness after one hour after the immersion is 0.141 μm , the area is 1439 μm 2 , and the volume is 200 μm 3 Yes , the thickness after 2 hours is 0.167 μm and the volume is 242 μm 3 , the thickness after 3 hours is 0.168 μm , the area is 1439 μm 2 , the volume is 239 μm 3 , and the volume and thickness Neither change was found, and it was found that this was not the composition for external use of the skin of the present invention. It can also be seen that in such a composition, the thickness or volume of the keratinocytes decreases after the immersion. This is because the moisture absorbed inside is released. When this was used by a person who had collected corneocytes, a rash occurred one week later, so the use was discontinued. From this, it can be seen that the discrimination result that the cosmetic of the discrimination method of the present invention is not suitable for this panelist was correct.
Polyethylene glycol 1 part by weight Glycerin 3 parts by weight Isoprene glycol 2 parts by weight Methylparaben 0.1 part by weight Water 93.9 parts by weight
【The invention's effect】
According to the present invention, it is possible to provide a method for distinguishing moisturizing action in consideration of physiological significance, and to provide an external composition for skin containing a corresponding moisturizing component.
Claims (5)
前記増大率が大きければ保湿作用が高く、前記増大率が小さければ保湿作用が低いと判定することを特徴とする、保湿作用の判定法。 Index keratinocytes, were immersed in the composition of the skin external including moisturizing, the thickness and / or volume of keratinocytes before and after immersion was measured, the thickness and / or volume increase rate of keratinocytes after immersion A method for determining the moisturizing action of the composition as
A method for determining a moisturizing effect, wherein the moisturizing effect is high if the increase rate is large, and the moisturizing effect is low if the increase rate is small.
前記増大率が大きい組成物を選択することを特徴とする、組成物の選択方法。 A method for selecting a composition, comprising selecting a composition having a large increase rate.
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| JP29606899A JP3715151B2 (en) | 1999-10-19 | 1999-10-19 | Method of distinguishing moisturizing ingredients and cosmetics |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29606899A JP3715151B2 (en) | 1999-10-19 | 1999-10-19 | Method of distinguishing moisturizing ingredients and cosmetics |
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| JP2001116745A JP2001116745A (en) | 2001-04-27 |
| JP3715151B2 true JP3715151B2 (en) | 2005-11-09 |
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| JP4876500B2 (en) * | 2005-09-22 | 2012-02-15 | 日油株式会社 | Gel hand sanitizer |
| KR100969564B1 (en) | 2008-07-07 | 2010-07-12 | (주)아모레퍼시픽 | How to measure skin moisture |
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