JP3719951B2 - Skin preparation - Google Patents

Description

【００４９】
【表２】

【００５０】
【表３】

【００５１】
表３より明らかなように、対照においては、ＵＶＢ照射日数が１０週を越える頃には皮膚に形成されたしわの深さは中程度にまで達し、２０週後には深いしわの形成が認められていた。有効成分として、カッコン抽出物又は抽出分画物のみを含有する比較例１及び比較例６塗布群では、２０週後に軽微なしわの形成が認められる程度で、しわの形成は良好に抑制されており、また抗炎症剤を含有し、カッコン抽出物又は抽出分画物もしくは4',7-ジヒドロキシイソフラボンを含有しない他の比較例塗布群でも、対照に比べてしわ形成に対する抑制効果は認められていたが、それぞれ対応する実施例塗布群では、いずれも２０週後に微小なしわの形成が認められた程度で、それぞれ対応する比較例塗布群に比べて、しわ形成に対する抑制効果は有意に向上していた。
【００５２】
続いて、本発明の実施例１〜実施例１２について使用試験を行い、皮膚の老化症状の改善効果を評価した。その際、実施例９〜実施例１２において、配合したカッコン抽出物又は抽出分画物及び抗炎症剤を表４に示すように代替して比較例９〜比較例１２とし、上記比較例１〜比較例８とともに同時に使用試験に供した。
【００５３】
【表４】

【００５４】
皮膚の老化症状の改善効果は、小じわ形成及び皮膚弾性の低下が顕著に認められる４０才代〜６０才代の女性パネラー２０名を１群とし、各群に実施例及び比較例のそれぞれをブラインドにて１日２回、２カ月間連続して使用させて評価した。小じわの程度については肉眼観察及び写真撮影により評価し、皮膚弾性についてはキュートメーターにより測定して、それぞれ使用試験開始前及び終了後の状態を比較し、「改善」，「やや改善」，「変化なし」の３段階で評価した。結果は、各評価を得たパネラー数にて表５に示した。
【００５５】
【表５】

【００５６】
表５より明らかなように、有効成分として抗炎症剤を含有するがカッコン抽出物等を含有しない比較例２〜比較例５，比較例７，比較例８及び比較例１０〜比較例１２の各使用群においては、小じわ及び皮膚弾性の改善傾向は認められるものの、明確な改善を認めたパネラーはさほど多くなかった。カッコンの抽出物又は抽出分画物のみを含有する比較例１，比較例６，及び比較例９の各使用群のそれぞれにおいては、かなり良好な小じわ及び皮膚弾性の改善傾向が認められていたが、実施例使用群においては、それぞれ対応する比較例使用群に比べて、明確な改善を認めたパネラーは有意に多くなっていた。
【００５７】
また、本発明の実施例１〜実施例１２及び比較例１〜比較例１２について、肌荒れ症状の改善効果を評価した。肌荒れ症状の改善効果は、顕著な肌荒れ症状を呈する２０才代〜６０才代の女性パネラー２０名を１群とし、各群に実施例及び比較例のそれぞれをブラインドにて１日２回、２カ月間連続して使用させて評価した。使用試験開始前及び終了後の皮膚の状態を、表６に示す評価基準に従って評価，点数化し、２０名の平均値を算出して表７に示した。
【００５８】
【表６】

【００５９】
【表７】

【００６０】
表７より明らかなように、本発明の実施例使用群ではいずれにおいても顕著な肌荒れの改善が認められ、使用試験終了後において、皮膚の状態はほぼ良好〜良好な状態にまで改善されていた。これに対し比較例使用群においても、かなり良好な肌荒れの改善が認められていたが、その程度はそれぞれ対応する実施例使用群に比べて小さいものであった。
【００６１】
なお実施例１〜実施例１２については、２５℃で６カ月間保存した場合において状態の変化は全く認められず、男性パネラー３０名による４８時間の背部閉塞貼付試験においても、問題となる皮膚刺激性反応は認められなかった。
【００６２】
【発明の効果】
以上詳述したように、本発明により、安定性及び安全性が良好で、肌荒れ及び皮膚の老化の防止，改善効果が相乗的に向上した皮膚外用剤を得ることができた。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an external preparation for skin which synergistically improves the effect of preventing and improving rough skin and aging of the skin. More specifically, an external preparation for skin comprising a combination of one or more selected from an extract and extract of cuckoo, or 4 ', 7-dihydroxyisoflavone, which is an active ingredient thereof, and an anti-inflammatory agent. About.
[0002]
[Prior art]
It is known that inflammatory reaction of the skin and aging of the skin progress due to aging, exposure to ultraviolet rays, oxidative stress due to reactive oxygen species generated in the skin tissue, contact with drugs and various allergens, and the like. In the field of topical skin preparations, many physiologically active ingredients such as reactive oxygen species scavengers, anti-inflammatory agents, and antiallergic agents have been searched and examined in order to prevent inflammation and aging of the skin. In addition, as it becomes clear that aging symptoms of skin progress due to modification of collagen, which is a component of the dermis matrix, due to aging and stress, studies on substances that have the effect of promoting their production in skin tissue Has been made. In recent years, there has been an increasing tendency to demand such components from plants, reflecting the natural and plant orientation of consumers.
[0003]
However, among the above-mentioned ingredients of plant origin that have already been reported, the activity is low, so that a considerably high concentration is required to obtain a sufficient action effect when blended with an external preparation for skin, stability or safety. When there are problems with sexuality, or there are things that give unfavorable color or odor to the external preparation for skin, when blended with external preparation for skin, all of the stability and safety of the preparation, and the action effect There are few things that can be satisfied in terms of the situation. In addition, since the inflammatory reaction and aging of the skin proceed with various factors involved in a complicated manner, sufficient effects cannot be obtained even if a substance that acts only on a part of the reaction is used.
[0004]
[Problems to be solved by the invention]
Accordingly, an object of the present invention is to obtain an external preparation for skin that suppresses complex skin inflammatory reactions and the like, and synergistically improves the effect of preventing and improving rough skin and aging of the skin.
[0005]
[Means for Solving the Problems]
As a result of various studies to solve the above-mentioned problems, the present inventors have found a high fibroblast activation action and a collagen production promotion action in the extract and extract fraction of cucumber ( Puerariae Radix ), and further selected from these 1 The combination of seeds or two or more and one or more anti-inflammatory agents in the topical skin preparation can prevent skin roughness and skin aging, and provide a synergistic improvement in the improvement effect. As a result, the present invention has been completed.
[0006]
In particular, the use of an extract with a high content of 4 ′, 7-dihydroxyisoflavone (daidzein) as a cuckoo extract and extract fraction provides a good effect, and can also be used in combination with 4 ′, 7-dihydroxyisoflavone itself. May be.
[0007]
In addition, as for the cuckoo extract, mucopolysaccharide fragmentation suppression, active oxygen elimination, antioxidant action (JP-A-6-24937), fat synthesis promoting action (JP-A-11-199499), hair papilla activating action (JP-A-11) -240823) has been disclosed, and hyaluronic acid production promoting action has also been reported (Abstracts of the 120th Annual Meeting of the Pharmaceutical Society of Japan 2, page 58, 2000), but the production promotion action of type I collagen is completely known. It is not done. Furthermore, a metabolite of 7-isopropoxyisoflavone has been reported to promote type I collagen synthesis (Calcified Tissue International 55 (5) 356-362 (1994)). Although it is well known that dihydroxyisoflavone has an antiacetylcholine action, no such report has been made so far.
[0008]
In addition, anti-inflammatory agents have long been blended in topical skin preparations with the expectation of improving skin roughness. However, the extract or extract of cuckoo or 4 ', 7-dihydroxyisoflavone and anti-inflammatory agents The effect of the present invention obtained by using together is not even suggested so far.
[0009]
DETAILED DESCRIPTION OF THE INVENTION
Cucon ( Puerariae Radix ) used in the present invention is a root excluding pericy of the deciduous Fujimoto ( Pueraria lobata Ohwi) belonging to the leguminosae ( Leguminosae ), Japanese, Korean and Chinese square brackets, Board brackets and powder brackets can be used.
[0010]
Cascon ( Puerariae Radix ) may be subjected to extraction as it is, but considering extraction efficiency, it is preferable to perform extraction after processing such as shredding, drying, and pulverization. Extraction is performed by immersing in an extraction solvent. In order to increase the extraction efficiency, stirring may be performed, or homogenization may be performed in an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but is suitably about 4 hours to 14 days.
[0011]
Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol, and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerin, and ethers such as ethyl ether and propyl ether. , Polar organic solvents such as esters such as ethyl acetate and butyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these are selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used.
[0012]
The extract from the above-mentioned solvent of cucumber ( Puerariae Radix ) can be incorporated into the external preparation for skin according to the present invention as it is, but the concentrated and dried solid can be dissolved again in water or a polar solvent, or these It may be used after performing purification treatment such as decolorization, deodorization, desalting, etc. within the range that does not impair the physiological action, or after fractionation treatment by column chromatography or the like. In particular, when adsorbed on an ion-exchange adsorbing resin and then eluted sequentially with a mixed solvent of water and ethanol, the fraction eluted with an aqueous solution of 50% to 99.5% by volume of ethanol contains 4 ', 7-dihydroxyisoflavone. It is contained most and is most preferably used from the viewpoint of promoting collagen production. As the ion exchange resin, DIAION MCI gel HP-20 (manufactured by Mitsubishi Chemical Corporation) and the like are preferably used. The extract of Kacon and its processed products and fractions can be freeze-dried after each processing and fractionation and dissolved in a solvent before use. It can also be used by encapsulating in vesicles such as liposomes or microcapsules.
[0013]
In the present invention, purified 4 ′, 7-dihydroxyisoflavone may be used in place of the above-described extract of cuckoo or a fraction thereof.
[0014]
In the present invention, the anti-inflammatory agent used in combination with one or more of the extracts and extract fractions of the above-mentioned cuckon ( Puerariae Radix ), or 4 ′, 7-dihydroxyisoflavone is used as cortisone, hydrocortisone, prednisolone, methyl Prednisolone, dexamethasone, betamethasone, triamcinolone, triamcinolone acetonide, fluocinolone acetonide, fluocinonide, beclomethasone and steroidal anti-inflammatory agents such as phosphate, propionate, acetate, succinate, salicylic acid and aspirin, Salicylic acid derivatives such as salicylamide, etenzamide and methyl salicylate, indoleacetic acid derivatives such as indomethacin and sulindac, pyrazolidinedione derivatives such as phenylbutazone and oxyphenbutazone, mefenamic acid and flufe Non-steroidal anti-inflammatory agents such as anthranilic acid derivatives such as namic acid, propionic acid derivatives such as ibuprofen, ketoprofen and naproxen, phenylacetic acid derivatives such as diclofenac, fenbufen and bufexamac, and benzothiazine derivatives such as piroxicam, glycyrrhizic acid and dipotassium glycyrrhizinate, Glycyrrhizic acid salts and derivatives such as monoammonium glycyrrhizinate, glycyrrhetinic acid and stearyl glycyrrhetinate, glycyrrhetinyl stearate, 3-succinyloxyglycyrrhetinic acid disodium salt and derivatives, guaiazulene, ethyl guaiazulene sulfonate, guaiazulene sulfone Azulene derivatives such as sodium acid and camazulene, allantoin, aloin, aloe Jin, shikonin and isobutyl shikonin, acetyl shikonin, shikonin derivatives such isovaleryloxy shikonin, ginsenoside R _a1, ginsenoside derivatives such as ginsenoside R _a2, Ginsenoside such Ginsenoside R _b1, and 20-glucoside Gin Seno glucoside R _f, paeoniflorin, Examples include peonol and peonoside derivatives such as peonoside and peonolide.
[0015]
As anti-inflammatory agent in the present invention, scutellaria root (Scutellariae Radix), licorice (Glycyrrhizae Radix), Sophora root (Sophorae Radix), Psycho (Bupleuri Radix), peony (Paeoniae Radix), Cimicifuga (Cimicifugae Rhizoma), gymnastics (Zizyphi Fructus ), Chimo ( Anemarrhenae Rhizoma ), Buttonpi ( Moutan Cortex ), Ryutan ( Gentianae Scabrae Radix ), Forsythiae Fructus, and other herbal medicines used as anti-inflammatory agents or extracts thereof can also be used. For the external preparation for skin according to the present invention, one or more of these anti-inflammatory agents are selected and used.
[0016]
In the present invention, one or more kinds of extracts and extracted fractions of cucumber ( Puerariae Radix ), or 4 ', 7-dihydroxyisoflavone and one or more kinds of anti-inflammatory agents are applied to the skin. Included in the base. The blending amount per total amount of the external preparation for skin is about 0.0001 to 5.0% by weight, 4 ′, 7-dihydroxy, although it depends on the preparation method of the extract or extract fraction of cucumber ( Puerariae Radix ). For isoflavones, about 0.00001 to 0.1% by weight, and for anti-inflammatory agents, depending on the type, it is appropriate to set them to about 0.0001 to 5.0% by weight.
[0017]
The external preparation for skin according to the present invention can be provided in various dosage forms such as lotions, emulsions, gels, creams, ointments, powders, granules and the like. In addition, skin cosmetics such as lotion, milky lotion, cream, beauty essence, packs, foundation cosmetics such as makeup base lotion and makeup base cream, foundations for each dosage form such as emulsion, oily, solid, etc. It can be provided as makeup cosmetics such as color and teak color, and body cosmetics such as hand cream, leg cream, neck cream and body lotion.
[0018]
In addition, skin external preparations according to the present invention include oil components, surfactants, moisturizers, pigments, UV absorbers, antioxidants, perfumes, anti-inflammatory agents, in addition to extracts such as Cucon ( Puerariae Radix ) and anti-inflammatory agents. General pharmaceutical ingredients such as antifungal agents and cosmetic raw materials, and physiologically active ingredients such as skin cell activators and whitening agents can be included.
[0019]
【Example】
Further, the features of the present invention will be described in detail with reference to examples.
[0020]
First, preparation of an extract and extract fraction of cucumber ( Puerariae Radix ) to be included in the external preparation for skin according to the present invention will be described.
[0021]
[Cuckoo extract 1]
200g of dried powder of cocoon is immersed in 1 liter of ethanol, left to stand at 20 ° C for 7 days and extracted. The extract is collected by filtration, concentrated, dried and freeze-dried. It was.
[0022]
[Cuckoo extract 2]
Chop 500 g, chop and extract in 2 liters of ethanol at 20 ° C. for 3 days, collect the extract by filtration, concentrate, dry, dissolve in 1 liter of glycerin, did.
[0023]
[Cuckoo extract 3]
250 g of the cocoon was dried and pulverized, immersed in 2 liters of a 50% by volume ethanol aqueous solution at 20 ° C. for 7 days, filtered, and the filtrate was recovered to obtain the cocoon extract 3.
[0024]
[Cracoon extract fractions 1-4]
500 g of cucumber ( Puerariae Radix ) is dried, ground, immersed in 2 liters of ethanol, left to stand at 20 ° C. for 7 days, extracted, filtered to collect the filtrate, then concentrated, dried and frozen dry. 36.0 g of this dry powder is dissolved in 500 ml of ethanol, 1500 ml of purified water is further added, 600 ml of DIAIONMCI gel HP-20 (manufactured by Mitsubishi Chemical Corporation) is added and stirred for 1 hour, and then filtered to remove the resin. It was collected and packed in a column, and eluted stepwise in a mixed solvent of water and ethanol. At that time, fractions eluted with 50% by volume ethanol aqueous solution, 70% by volume ethanol aqueous solution, 90% by volume ethanol aqueous solution and 99.5% by volume ethanol aqueous solution were collected and lyophilized, respectively, and the extracted fractions were extracted. 1-4.
[0025]
Moreover, what was marketed as a pharmaceutical or cosmetics was used for the anti-inflammatory agent mix | blended in the following Example of this invention. The preparation of the anti-inflammatory crude drug extract is described below.
[0026]
[Ougon extract]
300 g of Ogon ( Scutellariae Radix ) was dried, pulverized, added to 500 ml of ethanol, left standing at 20 ° C. for 10 days for extraction, and filtered to collect the filtrate. The filtrate was concentrated under reduced pressure and lyophilized to give the title extract.
[0027]
[Licorice extract]
500 g of licorice ( Glycyrrhizae Radix ) was dried, ground, and extracted in 1 liter of hot water for 2 hours. The filtrate was collected by filtration, then concentrated under reduced pressure and then lyophilized to obtain the title extract.
[0028]
[Cuzin extract]
500 g of kujin ( Sophorae Radix ) was dried, pulverized, immersed in 1 liter of 50 vol% ethanol aqueous solution, and extracted at 25 ° C. for 7 days. The filtrate was collected by filtration and used as the title extract.
[0029]
[Peonies extract]
550 g of peony ( Paeoniae Radix ) was dried, pulverized, immersed in 1 liter of 50% by volume ethanol aqueous solution, and extracted at 20 ° C. for 10 days with stirring. The filtrate was then collected by filtration, concentrated under reduced pressure, and lyophilized to give the title extract.
[0030]
[Tithus extract]
600 g of Taisou ( Zizyphi Fructus ) was dried, pulverized, immersed in 1 liter of a 50% by volume ethanol aqueous solution, and extracted at 25 ° C. for 7 days. The filtrate was collected by filtration and used as the title extract.
[0031]
[Button pi extract]
520 g of button pin ( Moutan Cortex ) was dried, pulverized, immersed in 1 liter of ethanol, allowed to stand at 10 ° C. for 14 days, and extracted. The filtrate was collected by filtration and used as the title extract.
[0032]
[Ryutan extract]
650 g of Ryutan ( Gentianae Scabrae Radix ) was dried, ground and extracted in 1 liter of hot water for 4 hours. The filtrate was collected by filtration, concentrated under reduced pressure, and lyophilized to give the title extract.
[0033]
[Forsythia extract]
Forsythiae Fructus (750 g) was pulverized, immersed in 1.2 liters of 1,3-butylene glycol, and extracted for 10 days at 15 ° C. with stirring. The filtrate was collected by filtration and used as the title extract.
[0034]
Then, the prescription of the Example about the skin external preparation which concerns on this invention is shown.
[0035]
[Example 1] Lotion agent (1) Ethanol 20.00 (wt%)
(2) Polyoxyethylene (60E.O.) hydrogenated castor oil 1.00
(3) Cuckoo extract 1 0.05
(4) Dipropylene glycol 5.00
(5) 1,3-butylene glycol 10.00
(6) Sodium guaiazulene sulfonate 0.20
(7) Methyl paraoxybenzoate 0.10
(8) Purified water 63.65
Manufacturing method: Add (2) and (3) to (1) and dissolve to make the alcohol phase. On the other hand, (4) to (7) are sequentially dissolved in (8) to obtain an aqueous phase. Add the alcohol phase to the water phase and stir and mix.
[0036]
[Example 2] Emulsion (1) Cetanol 1.00 (wt%)
(2) Beeswax 0.50
(3) Petrolatum 2.00
(4) Squalane 6.00
(5) Dimethylpolysiloxane 2.00
(6) Polyoxyethylene (20E.O.) sorbitan 1.00
Monostearate (7) Glyceryl monostearate 1.00
(8) Glycerin 4.00
(9) 1,3-butylene glycol 4.00
(10) Methyl paraoxybenzoate 0.10
(11) Purified water 62.18
(12) Carboxyvinyl polymer 10.00
(1.0 wt% aqueous solution)
(13) Potassium hydroxide (10.0 wt% aqueous solution) 1.00
(14) Allantoin 0.20
(15) Ethanol 5.00
(16) Cuckoo extract fraction 1 0.02
Production method: The oil phase components (1) to (7) are mixed and dissolved by heating to 75 ° C. On the other hand, the water phase components (8) to (11) are mixed and dissolved to 75 ° C. After preliminarily emulsifying by adding the oil phase to this, (12) was added and uniformly emulsified with a homomixer, and then (13) was added to increase the viscosity, followed by cooling at 40 ° C. (14) Are added and mixed, then (16) is dissolved in (15) and added and mixed.
[0037]
[Example 3] Emulsion (1) Cetanol 1.0 (wt%)
(2) Beeswax 0.5
(3) Vaseline 2.0
(4) Squalane 6.0
(5) Dimethylpolysiloxane 2.0
(6) Polyoxyethylene (20E.O.) sorbitan 1.0
Monostearate (7) Glyceryl monostearate 1.0
(8) Stearyl glycyrrhetinate 0.2
(9) Glycerin 4.0
(10) 1,3-butylene glycol 4.0
(11) Methyl paraoxybenzoate 0.1
(12) Purified water 61.9
(13) Carboxyvinyl polymer 10.0
(1.0 wt% aqueous solution)
(14) Potassium hydroxide (10.0 wt% aqueous solution) 1.0
(15) Ethanol 5.0
(16) Cuckoo extract 1 0.1
(17) Ogon extract 0.2
Production method: The oil phase components (1) to (8) are mixed and dissolved by heating to 75 ° C. On the other hand, the aqueous phase components (9) to (12) are mixed and dissolved to 75 ° C. After preliminarily emulsifying by adding the oil phase to this, (13) was added and uniformly emulsified with a homomixer, and then (14) was added to increase the viscosity, followed by cooling at 40 ° C. (16) , (17) are dissolved in (15) and added.
[0038]
[Example 4] Oil-in-water cream (1) Beeswax 6.00 (wt%)
(2) Cetanol 5.00
(3) Reduced lanolin 8.00
(4) Squalane 27.50
(5) Glyceryl fatty acid ester 4.00
(6) Lipophilic glyceryl monostearate 2.00
(7) Polyoxyethylene (20E.O.) sorbitan 5.00
Monolaurate (8) Stearyl glycyrrhetinate 0.25
(9) Propylene glycol 5.00
(10) Methyl paraoxybenzoate 0.10
(11) Cuckoo extract fraction 2 0.02
(12) Purified water 36.93
(13) Kujin extract 0.20
Manufacturing method: The oil phase components (1) to (8) are mixed and dissolved to 75 ° C. On the other hand, (10) and (11) are dissolved in (9), added to (12), mixed and dissolved, and heated to 75 ° C. Next, the oil phase component is added to the aqueous phase component and pre-emulsified, and then uniformly emulsified with a homomixer. After cooling, (13) is added and mixed at 40 ° C.
[0039]
[Example 5] Oil-in-water cream (1) Beeswax 6.00 (wt%)
(2) Cetanol 5.00
(3) Reduced lanolin 8.00
(4) Squalane 27.50
(5) Glyceryl fatty acid ester 4.00
(6) Lipophilic glyceryl monostearate 2.00
(7) Polyoxyethylene (20E.O.) sorbitan 5.00
Monolaurate (8) Propylene glycol 5.00
(9) Methyl paraoxybenzoate 0.10
(10) Cuckoo extract fraction 3 0.02
(11) Purified water 36.38
(12) Bufexamac 1.00
Production method: The oil phase components (1) to (7) are mixed and dissolved to 75 ° C. On the other hand, (9) and (10) are dissolved in (8), mixed and dissolved in (11), and heated to 75 ° C. Next, the oil phase component is added to the aqueous phase component and pre-emulsified, and then uniformly emulsified with a homomixer. After cooling, (12) is added and mixed at 40 ° C.
[0040]
[Example 6] Gel agent (1) Dipropylene glycol 10.00 (wt%)
(2) Carboxyvinyl polymer 0.50
(3) Potassium hydroxide (10.0 wt% aqueous solution) 1.00
(4) Methyl paraoxybenzoate 0.10
(5) Cuckoo extract fraction 3 0.02
(6) Licorice extract 0.30
(7) Purified water 88.08
Production method: (2) and (6) are uniformly dissolved in (7), (4) and (5) are dissolved and added to (1), and then (3) is added to increase the viscosity.
[0041]
[Example 7] Oil-in-water emulsion type ointment (1) White petrolatum 25.0 (% by weight)
(2) Stearyl alcohol 25.0
(3) Glycerin 12.0
(4) Sodium lauryl sulfate 1.0
(5) Methyl paraoxybenzoate 0.1
(6) Purified water 34.4
(7) 1.0 (w / v)% of parentheses extracted fraction 4 1.5
Ethanol solution (8) Buttonpi extract 1.0
Production method: The oil phase components (1) to (4) are mixed and heated to dissolve uniformly to 75 ° C. On the other hand, the aqueous phase components (5) and (6) are mixed and heated to 75 ° C. The oil phase component is gradually added to the aqueous phase component while stirring to emulsify, and after cooling, (7) and (8) are added and mixed at 40 ° C.
[0042]
[Example 8] Liposome agent (1) Glycerin 2.0 (wt%)
(2) 1,3-butylene glycol 3.0
(3) Polyoxyethylene (25E.O.) oleyl ether 0.2
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Purified water 79.7
(7) 4 ', 7-dihydroxyisoflavone, peony 5.0
Extract encapsulated liposome production method: Dissolve (5) in (4), add to (6) together with (1) to (3), mix uniformly, add (7) to this and disperse. The liposome encapsulating 4 ', 7-dihydroxyisoflavone and peony extract of (7) is 1.0 (w / v)% of 4', 7-dihydroxyisoflavone and 2.0 (w / v)% of peony extract. 80 g of soybean lecithin was added to 100 ml of a 50 vol% ethanol aqueous solution containing and suspended at 55 ° C., followed by sonication to prepare liposomes, which were collected by centrifugation.
[0043]
[Example 9] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (wt%)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglyceryl dioleate 5.0
(5) Sodium L-glutamate 1.6
(6) L-serine 0.4
(7) Propylene glycol 3.0
(8) Methyl paraoxybenzoate 0.1
(9) Cuckoo extract 2 0.5
(10) Forsythia extract 1.5
(11) Purified water 50.8
(12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C, and gradually add to (4) heated to 50 ° C in advance with stirring. These are mixed in advance and uniformly dispersed in (1) to (3) heated and dissolved at 70 ° C. (7) to (10) are added to the remainder of (11), and the mixture heated to 70 ° C. is added with stirring and emulsified with a homomixer. After cooling, add and mix (12) at 40 ° C.
[0044]
[Example 10] Make-up base cream (1) Stearic acid 12.00 (wt%)
(2) Cetanol 2.00
(3) Glyceryl tri-2-ethylhexanoate 2.50
(4) Self-emulsifying glyceryl monostearic acid 2.00
Ester (5) Propylene glycol 10.00
(6) Cuckoo extract 1 0.02
(7) Methyl paraoxybenzoate 0.10
(8) Ryutan extract 0.05
(9) Potassium hydroxide 0.30
(10) Purified water 68.43
(11) Titanium oxide 2.00
(12) Bengala 0.40
(13) Yellow iron oxide 0.10
(14) Fragrance 0.10
Manufacturing method: The oil phase components (1) to (4) are mixed and dissolved to 75 ° C. On the other hand, (6) and (7) are dissolved in (5) and added to (10) together with (8) and (9), mixed and heated to dissolve, and the pigment components (11) to (13) are added to this. Add and disperse uniformly with homomixer to 75 ° C. Next, the oil phase component is added to the water phase component, and the mixture is uniformly emulsified with a homomixer. After cooling, (14) is added and mixed at 40 ° C.
[0045]
[Example 11] Emulsion foundation (1) Stearic acid 2.00 (wt%)
(2) Squalane 5.00
(3) Octyl dodecyl myristate 5.00
(4) Cetanol 1.00
(5) Decaglyceryl monoisopalmitate 9.00
(6) 1,3-Butylenecricol 6.00
(7) Methyl paraoxybenzoate 0.10
(8) Potassium hydroxide 0.08
(9) Purified water 51.57
(10) Titanium oxide 9.00
(11) Talc 7.40
(12) Bengala 0.50
(13) Yellow iron oxide 1.10.
(14) Black iron oxide 0.10
(15) Cuckoo extract 3 1.00
(16) Isolate extract 1.00
(17) Fragrance 0.15
Manufacturing method: The oil phase components (1) to (5) are mixed and dissolved to 75 ° C. On the other hand, the aqueous phase components (6) to (9) are mixed, dissolved by heating, the pigment components (10) to (14) are added thereto, and the mixture is uniformly dispersed with a homomixer to 75 ° C. Next, the oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified with a homomixer. After cooling, (15) to (17) are added and mixed at 40 ° C.
[0046]
[Example 12] Hand cream (1) Cetanol 4.00 (wt%)
(2) Vaseline 2.00
(3) Liquid paraffin 10.00
(4) Glyceryl monostearate 1.50
(5) Polyoxyethylene (60E.O.) glyceryl 2.50
Isostearic acid ester (6) Tocopherol acetate 0.25
(7) Glycerin 20.00
(8) Methyl paraoxybenzoate 0.10
(9) Cuckoo extract fraction 1 0.01
(10) Dipotassium glycyrrhizinate 0.02
(11) Purified water 59.62
Production method: The oil phase components (1) to (6) are mixed and dissolved to 75 ° C. On the other hand, (8) and (9) are dissolved in (7) and added to (11) together with (10), and mixed and dissolved to obtain 75 ° C. Next, the oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified with a homomixer and cooled.
[0047]
Among the examples according to the present invention described above, Examples 1 to 8 were evaluated for the effect of suppressing wrinkle formation on the skin by medium wavelength ultraviolet rays (UVB). At that time, in Example 1 to Example 8, as shown in Table 1, the konkon extract or fraction and the anti-inflammatory agent, or 4 ′, 7-dihydroxyisoflavone and peony extract-encapsulating liposomes were replaced. Comparative Examples 1 to 8 were evaluated at the same time. The evaluation was performed by taking 5 hairless mice as a group, applying 0.2 g of each of the examples and comparative examples to the back part once a day for each group, and applying UVB of 100 mJ / cm ² / times 3 times a week. Irradiated for 20 weeks, the state of wrinkle formation in the hairless mouse skin was observed, scored according to the criteria shown in Table 2, and evaluated. At this time, a group to which only purified water was applied was used as a control. As a result, the average value of each group was calculated and shown in Table 3 according to the relationship with the number of days of UVB irradiation.
[0048]
[Table 1]

[0049]
[Table 2]

[0050]
[Table 3]

[0051]
As is apparent from Table 3, in the control, when the UVB irradiation days exceeded 10 weeks, the depth of wrinkles formed in the skin reached a medium level, and after 20 weeks, formation of deep wrinkles was observed. It was. In the application group of Comparative Example 1 and Comparative Example 6 containing only the cool extract or extract fraction as an active ingredient, the formation of wrinkles was suppressed to the extent that slight wrinkle formation was observed after 20 weeks. In addition, an inhibitory effect on wrinkle formation was recognized in the other application group containing an anti-inflammatory agent and not containing a cuckoo extract or extract fraction or 4 ′, 7-dihydroxyisoflavone as compared with the control. However, in each of the corresponding example application groups, the formation of fine wrinkles was observed after 20 weeks, and the inhibitory effect on wrinkle formation was significantly improved compared to the corresponding comparative application group. It was.
[0052]
Then, the use test was done about Example 1-Example 12 of this invention, and the improvement effect of the skin aging symptom was evaluated. At that time, in Example 9 to Example 12, the blended cuckoo extract or extract fraction and the anti-inflammatory agent were replaced as shown in Table 4 to give Comparative Examples 9 to 12, and Comparative Examples 1 to Simultaneously with the comparative example 8, it used for the use test.
[0053]
[Table 4]

[0054]
The effect of improving skin aging symptoms is that 20 female panelists in their 40's to 60's in which fine wrinkle formation and skin elasticity are markedly reduced are considered as one group, and each of the examples and comparative examples is blinded in each group. And used continuously twice a day for 2 months. The degree of fine lines is evaluated by visual observation and photography, and the skin elasticity is measured by a cut meter, and the state before and after the use test is started and compared, respectively. “Improved”, “Slightly improved”, “Change” It was evaluated in three stages, “none”. The results are shown in Table 5 in terms of the number of panelists that obtained each evaluation.
[0055]
[Table 5]

[0056]
As is apparent from Table 5, each of Comparative Examples 2 to 5, Comparative Example 7, Comparative Example 8 and Comparative Example 10 to Comparative Example 12 containing an anti-inflammatory agent as an active ingredient but not containing a cuckoo extract or the like. In the use group, although there was a tendency to improve fine wrinkles and skin elasticity, there were not so many panelists that showed a clear improvement. In each of the use groups of Comparative Example 1, Comparative Example 6, and Comparative Example 9 containing only the extract of Kakkon or the extract fraction, a fairly good tendency to improve fine wrinkles and skin elasticity was observed. In the Example use group, the number of panelists who recognized a clear improvement was significantly greater than in the corresponding Comparative Example use group.
[0057]
Moreover, the improvement effect of the rough skin symptom was evaluated about Example 1- Example 12 and Comparative Example 1- Comparative Example 12 of this invention. The improvement effect of rough skin symptoms is a group of 20 female panelists in their 20s to 60s who show remarkable rough skin symptoms, and each of the examples and comparative examples is blinded twice a day. Evaluated by using continuously for months. The skin condition before and after the start of the use test was evaluated and scored according to the evaluation criteria shown in Table 6, and the average value of 20 people was calculated and shown in Table 7.
[0058]
[Table 6]

[0059]
[Table 7]

[0060]
As is clear from Table 7, in the examples using group of the present invention, remarkable improvement in rough skin was observed in any of the groups, and after completion of the use test, the skin condition was improved from almost good to good. . On the other hand, in the comparative example use group, a considerably good improvement in rough skin was observed, but the degree was smaller than the corresponding example use group.
[0061]
In Examples 1 to 12, no change in state was observed when stored at 25 ° C. for 6 months, and the skin irritation was problematic even in a 48-hour back obstruction test with 30 male panelists. Sexual reaction was not observed.
[0062]
【The invention's effect】
As described above in detail, according to the present invention, it was possible to obtain an external preparation for skin having excellent stability and safety, and synergistically improving effects of preventing and improving rough skin and aging of the skin.

Claims (3)

One or more selected from extracts and extracts of cuckoo (Puerariae Radix), Scutellariae Radix, Glycyrrhizae Radix, Sophorae Radix , Zizyphi Fructus , Buttonpi ( A skin external preparation for improving rough skin, comprising one or more herbal medicines selected from Moutan Cortex), Ryutan (Gentianae Scabrae Radix), and Forsythiae Fructus.   The extract fraction of cucumber (Puerariae Radix) is eluted with 50% to 99.5% by volume ethanol aqueous solution when the extract is adsorbed on ion-exchange adsorption resin and then eluted with water / ethanol mixed solvent. The skin external preparation for rough skin improvement according to claim 1, which is a fraction obtained.   The skin external preparation for improving rough skin according to claim 1, wherein the extracted fraction of cuckon is 4 ', 7-dihydroxyisoflavone.