JP3945580B2 - Control of soil pests by low-voltage AC electricity - Google Patents
Control of soil pests by low-voltage AC electricity Download PDFInfo
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Description
【0001】
【発明の属する技術分野】
本発明は、農業もしくは園芸における土壌病害虫の制御法に関する。
【0002】
【従来の技術】
作物の連作による土壌病害虫の防除は、地上部の病害虫に比べ困難である。多くの場合、強力な農薬を多量に使用して病害虫を殺滅する方法が採られている。その他、大量の堆肥や有機質資材を投入して土壌中の生物間拮抗作用を利用して対応する方法や、寄生性が低い、もしくは病害虫に強い抵抗性品種を導入する方法が採用されている。また、大規模農業地帯では分類上全く異なった種類の作物を栽培する輪作によって対応している。
【0003】
また、マツノザイセンチュウの防除に高周波、高電圧を松の樹幹に付加することも行われている。
【0004】
【発明が解決しようとする課題】
現在農耕地における土壌病害虫防除の主体は、クロルピクリン、臭化メチル、1,3−ジクロロプロペン、メチルイソチオシアネートなどの農薬である。しかし、これらの薬剤は何れも気化しやすく、強い刺激性を有しており、また大量に使用されるため地下水や大気を汚染する恐れがある。更に臭化メチルはオゾン層を破壊する物質として2005年以降、世界の農耕地で使用が禁止される。
【0005】
有機農業においてはこれらの農薬の代わりに10アール当たり数トンの堆肥等の有機質素材を投下して土壌内の微生物を増加、多様化させることによって被害の回避を図っている。しかし、一方でこれらの大量有機質素材の投入は土壌塩類の濃度を上昇させ、地下水に肥料成分が溶出する等問題を生じている。その他、抵抗性品種の導入は、嗜好に対する優良形質が失われたり、抵抗性を打破する系統の微生物が発生したりして満足しうる病害虫の対抗手段になり得ていない。輪作は、栽培面積が狭小で、経済性を重視せざるを得ない集約農業においては利用することが困難である。
【0006】
また、マツノザイセンチュウの防除に検討されている高周波、高電圧を付加する方法を一般農耕地に導入することは、コストはもとより安全性を確保する上からも難しい。
【0007】
【課題を解決するための手段】
直接電気を利用して生物活動を制御する事例は、農業における加温機や照明による作物の生育促進、光による害虫の誘引などの一般的な方法がある。その他、次のようなことが知られている。例えば、化学と生物(2000,Vol.38,NO.8,503−507)には、電気刺激によって細胞機能の制御ができることが記されている。また、ネマトロジー(Nematology,2001,Vol.3(1),31−34)には化学物質に接触した土壌線虫が一定の電位差パルスを発生することが記載されている。
【0008】
本発明者による、平板寒天上のサツマイモネコブセンチュウへの交流電気50V以下の付加は、行動や生存に影響を与えなかった。また、100Vの電圧を付加した場合は、激しく痙攣して数分以内に全ての個体が死亡した、この死亡原因は発熱によるものと推測された。一方、直流を流した場合は、電極に用いた銅の溶出など系内のイオン分布に変化を生じ死亡個体の発生が認められた。
【0009】
開放系の農耕地土壌に電気を用いる場合、そこに入る人間や動物に対する安全の確保や作物に対する影響を回避する必要がある。既存の報告から、直流電気ではやや許容度が高いとされるが、交流電気で生命に対して殆ど影響がないとされるのは、電圧30V以下、電流0.05A以下である。また、30V以上の土壌への電圧付加は発熱を起こし、土壌温度が30℃を越える環境下の作物栽培においては高温障害を生じて使用出来ないことが明らかになった。一方、土壌に直流電気を連続的に通電した場合、電気化学反応により土壌中の水素イオン濃度等、物質濃度を不均一化して作物の生育を大きく阻害し利用できないことを見いだした。また、土壌病害虫の発生を抑制する他、電圧の付加によって作物の葉が濃緑化し肥料吸収量の増加が示唆された。
【0010】
以上のような課題を回避し、同時に、土壌病害虫の発生を抑制する方法として、30V以下の交流電気を連続的もしくは断続的に土壌に通電することで解決しうることを見いだした。また、電気化学反応によって土壌内の物質濃度を不均質化させる直流電気は、一定時間毎に電流の方向を逆転させる回路により低周波交流電気に変換して通電する方法を見いだした。
【0011】
電源は配線されている交流電源を利用するのが最も簡単であるが、風力発電装置、太陽電池を用いることもできる。電圧は変圧器を用いることにより容易に変えることができる。断続電圧付加は、タイマーを使うことによって自由な断続時間が得られる。またサイリスタ等の電流をカットする器具を使うことによって極短時間の断続も可能である。電圧の付加は栽培の全期間に及ぶ必要はない。一般に土壌病害虫は作物の生育段階、気候、季節によって消長があり、費用削減のためにも適期の通電が望ましい。
【0012】
直流を自由な周期を持つ交流に変換するには、タイマーを用いて電磁リレーを設定時間毎に開閉させ、リレーに組み込んだ直流回路の陽極と陰極を交互に入れ替えることによって得ることができる。電極材料としては腐食しにくい銅製やステンレススチール製の板、網、もしくはこれらの切削屑など螺旋状、毛状のもの、アルミニウムコーティングフィルムなど埋設が容易で表面積を大きくとることが可能なものが好ましい。
【0013】
【発明の実施の形態】
発明の実施の形態を実施例に基づき説明する。
【0014】
【実施例1】
シャーレ内電圧付加による線虫の行動
水道水1リッターに食塩5g、及び寒天12gを加熱溶解し、その約20mlを9cmガラスシャーレに流して平板を作成した。このシャーレの中心部に約20頭のサツマイモネコブセンチュウ(Meloidogyne incognita)のII期幼虫を含む懸濁水200マイクロリッターを滴下した。シャーレの両端に7cmの距離をおいて平行になるように銅製電極を深さ0.7cm、幅1.5cm埋設させた。可変変圧器(山菱電気社製 S−130−1)、可変整流器(Skynie社製 BA−163)、及びテスター(アロー電子工業社製ETU−102A)を用いて電圧を各レベルに調整して、交流電気、もしくは直流電気を流した。通電から5分間、顕微鏡を用いて線虫の行動を観察した。一部はその後の状態を継続観察した。その結果を表1に示す。
【0015】
【表1】
上表のように、交流の100V電圧付加は寒天の発熱により全個体が死亡したが、50V以下では致死的影響を与えなかった。また、直流の通電は走化性を示す結果が得られた。直流による線虫の致死効果は電気化学反応によるものと判断された。
【0016】
【実施例2】
電圧付加によるセンチュウ寄生度への影響
直径18cmの素焼鉢にサツマイモネコブセンチュウに汚染されたクロボク土壌(247±80頭/20g土壌、3反復、ベルマン法)850mlを詰め、10cmの距離をおいて平行になるように幅5cm長さ10cmの銅板を埋設した。電極間に3葉期のトマト苗(品種 タイニーティム)を5本定植して潅水した。100V交流電源を変圧器(豊澄電源機器社製 P−4106及びHT−1203)を用いて測定値7Vの連続、測定値14Vの断続通電を行った。また、調光用サイリスタ(太洋電機産業社製 PC−30)を調整して土壌に埋設した電極間電圧の測定値を15Vに設定して通電した。交流14Vの断続通電はタイマー(オムロン社製 H3CR)を変圧器の前に接続し、0.05秒毎にオンオフして得た。1週間後に500倍に希釈した液肥(ハイポネクスジャパン社製 微粉ハイポネクス)を散布した。4週間後にトマトを堀上げ、ネコブ寄生度、茎葉重、及びpHメータ(堀場製作所社製 B−211)を用いて土壌pHを調査した。ネコブ寄生度は0(ネコブを認めない)から1,2,3,4(根系のほぼ75%以上にネコブが認められる)の4段諧として、各段階は中間位を設けた。実験は無加温ハウス内で行った。実験期間中の平均温度は27.6℃であった。結果の平均値を表2に示す。
【0017】
【表2】
以上のように交流電気の連続、もしくは断続付加はネコブ寄生度を低下させた。
使用電力量を等しくした7V連続と14V断続間でネコブ寄生度に大きな差は認められなかった。サイリスタを用いた断続付加のネコブ寄生度はやや低い傾向を示した。一方、通電した全ての区で線虫対照区に比べて茎葉重の低下が認められ、高温時の電圧付加は作物に対して生育を阻害する可能性が示唆された。
【0018】
【実施例3】
電圧付加とセンチュウ寄生度及びトマト斑点病の抑制
直径18cmの素焼鉢にクロボク土壌850mlを詰め、10cmの距離をおいて平行になるように幅5cm長さ10cmの銅板を埋設した。電極間に2葉期のトマト苗(品種 タイニーティム)を5本定植した。サツマイモネコブセンチュウのII期幼虫約10000頭を含む懸濁液10mlを土壌表面に均一になるよう接種して潅水した。潅水直後の電極間の抵抗値は250Ωであった。可変変圧器と可変整流器を用いて、交流、直流をそれぞれ6Vに変換して通電した。交流はタイマーを用いて0.05秒毎にオンオフして断続的に通電した。1週間後に500倍に希釈した液肥(ハイポネクスジャパン社製 微粉ハイポネクス)を散布した。1週間経過前後からトマト斑点病の発症が全体に認められた。2週間後にトマト第1、第2本葉の斑点病発症葉数を調査した。発病葉数の調査後殺菌剤(マンゼブ水和剤)500倍液を散布した。3週間後に土壌pHを測定した。6週間後にトマトを堀上げてネコブ寄生度、茎葉重、卵嚢形成状況、電極の状態を調査した。ネコブ寄生度は0(ネコブを認めない)から1,2,3,4(根系のほぼ75%以上にネコブが認められる)の4段階として、各段階は中間位を設けた。実験は無加温ハウス内で行った。実験期間中の平均温度は23.3℃であった。結果の平均値を表3に示す。
【0019】
【表3】
以上のように6Vの断続交流電圧、もしくは直流電圧の付加は、トマト斑点病(Stemphylium lycopersici)の発症、及びサツマイモネコブセンチュウのトマト根への侵入を抑制した。同時に交流を付加した区はトマト葉の濃緑化現象が認められた。一方、直流の付加は殆どの株が枯死し、茎葉重も低下して通電によるトマトの生育阻害は顕著であった。原因はpHの変化によるものと推測された。また、卵嚢の形成阻害は認められず次世代に影響を与えないと判断された。
【0020】
【実施例4】
上限電圧の付加とセンチュウ寄生度
直径18cmの素焼鉢にサツマイモネコブセンチュウに汚染されたクロボク土壌(21±6頭/20g土壌、3反復、ベルマン法)850mlを詰め、10cmの距離をおいて平行になるように幅5cm長さ10cmの銅板を埋設した。電極間に4葉期のトマト苗(品種 タイニーティム)を5本定植して潅水した。100V交流電源を変圧器(豊澄電源機器社製 HT−3002)により30Vに変換して断続通電した。また、調光用サイリスタを用い、電極間電圧の測定値を15V、及び30Vに設定して通電した。交流30V断続通電はタイマーを変圧器の前に接続し、0.05秒毎にオンオフした。1週間後に500倍に希釈した液肥(ハイポネクスジャパン社製 微粉ハイポネクス)を散布した。定植直後の日中及び翌朝、通電による地温変化を測定した。7週間後にトマトを堀上げてネコブ寄生度、線虫密度、茎葉重を調査した。ネコブ寄生度は0(ネコブを認めない)から1,2,3,4(根系のほぼ75%以上にネコブが認められる)の4段階として、各段階は中間位を設けた。実験は無加温ハウス内で行った。実験期間中の平均温度は22.9℃であった。結果の平均値を表4に示す。
【0021】
【表4】
以上のように15V以上の電圧を付加した場合、いずれも地温上昇が認められ、サイリスタの測定電圧値30Vの場合は、地温が40℃を越えて1日後にトマトが萎凋枯死したため最終調査までいたらなかった。30V断続通電、及びサイリスタの測定電圧値15V通電は約3℃の地温上昇が認められたが、トマトの生育に影響を与えなかった。いずれも通電によりネコブ寄生度、及び第二世代線虫密度を低下させた。しかし、サイリスタの15V通電区はネコブ寄生度の低下にも関わらずトマト茎葉重の減少が認められた。
【0022】
【実施例5】
低周波交流付加とネコブ寄生度及びトマトの立ち枯れ率
直径18cmの素焼鉢にサツマイモネコブセンチュウに汚染されたクロボク土壌(63±21頭/20g土壌、3反復、ベルマン法)850mlを詰め、10cmの距離をおいて平行になるように幅5cm長さ10cmの銅板を埋設した。電極間に3葉期のトマト苗(品種 タイニーティム)を5本定植し、サツマイモネコブセンチュウのII期幼虫約15000頭を接種して潅水した。100V交流電源を可変変圧器と整流器(東洋計器社製 A20640j)を用いて0.7Vの直流に変換した後、更に電磁リレー(オムロン社製 LY2)回路に組み込んで1時間、1日、1週間毎に陽極と陰極を交互に入れ替える低周波交流を調整して通電した。また、1週間交互0.05秒断続通電は1週間ごとに陽極、陰極を入れ替えると同時にタイマーを変圧器の前に接続して0.05秒毎にオンオフした。1週間交互1週間断続通電は1週間毎に通電を断続し、通電毎に陽極、陰極を入れ替えた。対照として0.7V直流の連続通電区を設けた。1週間後に500倍に希釈した液肥(ハイポネクスジャパン社製 微粉ハイポネクス)を散布した。4週間後に本葉の数を調査した。立ち枯れ病による萎凋株は随時抜き取りネコブ寄生度を調査した。8週間後にトマトを堀上げてネコブ寄生度、茎葉重を調査した。また、試験紙(東洋濾紙社製 万能pH1−11)でpHを測定した。ネコブ寄生度は0(ネコブを認めない)から1,2,3,4(根系のほぼ75%以上にネコブが認められる)の4段階として、各段階の中間位を設けた。実験は加温ハウス内で行った。実験期間中の平均温度は16.8℃であった。結果の平均値を表5に示す。
【0023】
【表5】
上表中、ネロブ寄生度に枯死株を含み、茎葉重に枯死株を含まない。
以上のように低電圧の直流を長い周期を有する交流に変換することによって直流を連続通電した場合に発生する作物の生育阻害作用を防止することが可能になった。同時に、立ち枯れ病の発生やネコブ寄生度を低下させることが認められた。また、サツマイモネコブセンチュウ密度が高く、かつ栽培温度が低い本条件下においては、全ての個体でリン酸欠乏症状である茎葉の赤紫色化が認められた。
【0024】
【発明の効果】
人体、作物に安全な30V以下で種々の周期性を持った交流電気を土壌に連続的、もしくは断続的に通電することによって土壌病害虫の被害を抑制する。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a method for controlling soil pests in agriculture or horticulture.
[0002]
[Prior art]
Control of soil pests by continuous cropping is more difficult than pests on the ground. In many cases, a method of killing pests using a large amount of a strong pesticide is employed. In addition, a method of responding by using a large amount of compost and organic materials and utilizing the antagonism between organisms in the soil, and a method of introducing a resistant variety with low parasitic properties or strong resistance to pests are employed. Also, in large-scale agricultural areas, this is handled by rotating crops that cultivate a completely different type of crop.
[0003]
In addition, high frequency and high voltage are added to pine tree trunks for controlling pinewood nematodes.
[0004]
[Problems to be solved by the invention]
At present, pesticides such as chloropicrin, methyl bromide, 1,3-dichloropropene, and methyl isothiocyanate are mainly used to control soil pests in farmland. However, any of these drugs is easy to vaporize, has a strong irritation, and is used in large quantities, there is a risk of contaminating groundwater and air. In addition, methyl bromide is prohibited from being used in agricultural fields around the world since 2005 as a substance that destroys the ozone layer.
[0005]
In organic agriculture, instead of these pesticides, organic materials such as compost of several tons per 10 ares are dropped to increase and diversify microorganisms in the soil, thereby avoiding damage. On the other hand, however, the introduction of these large quantities of organic materials raises problems such as increasing the concentration of soil salts and eluting fertilizer components into groundwater. In addition, the introduction of resistant varieties cannot be a satisfactory countermeasure against pests because the superior traits for preference are lost or the microorganisms of strains that break resistance are generated. Rotation is difficult to use in intensive agriculture where the cultivation area is small and economic efficiency must be emphasized.
[0006]
In addition, it is difficult to secure safety as well as cost to introduce a method for applying high frequency and high voltage, which is being studied for controlling pinewood nematodes, to general farmland.
[0007]
[Means for Solving the Problems]
Examples of directly controlling biological activities using electricity include general methods such as promotion of crop growth by heating and lighting in agriculture and attraction of pests by light. In addition, the following is known. For example, Chemistry and Biology (2000, Vol. 38, NO. 8, 503-507) describes that cell functions can be controlled by electrical stimulation. In addition, nematology (Nematology, 2001, Vol. 3 (1), 31-34) describes that soil nematodes in contact with chemical substances generate a constant potential difference pulse.
[0008]
The addition of AC electricity of 50 V or less to sweet potato root nematodes on flat agar by the present inventor did not affect behavior or survival. Moreover, when a voltage of 100 V was applied, all individuals died within a few minutes due to severe convulsions, and it was assumed that the cause of this death was due to fever. On the other hand, when direct current was passed, the distribution of ions in the system changed, such as the elution of copper used for the electrodes, and deaths were observed.
[0009]
When electricity is used in open farmland soil, it is necessary to ensure the safety of humans and animals entering the soil and avoid the impact on crops. According to existing reports, it is said that the tolerance is somewhat high with DC electricity, but it is voltage 30V or less and current 0.05A or less that AC electricity has almost no influence on life. In addition, it was clarified that application of voltage to soil of 30 V or higher generates heat and cannot be used in crop cultivation under an environment where the soil temperature exceeds 30 ° C. due to high temperature damage. On the other hand, it was found that when direct current electricity was applied to the soil, the growth of crops was greatly hindered by making the concentration of substances such as hydrogen ion concentration in the soil non-uniform due to the electrochemical reaction. In addition to suppressing the occurrence of soil pests, the addition of voltage suggested that the leaves of the crop became dark green and increased fertilizer absorption.
[0010]
As a method of avoiding the above problems and simultaneously suppressing the occurrence of soil pests, it has been found that AC electricity of 30 V or less can be solved by energizing the soil continuously or intermittently. In addition, we found a method to convert the direct current electricity, which makes the material concentration in the soil inhomogeneous by electrochemical reaction, into low frequency alternating current electricity by a circuit that reverses the direction of the current at regular intervals.
[0011]
Although it is easiest to use a wired AC power source as the power source, a wind power generator or a solar cell can also be used. The voltage can be easily changed by using a transformer. For the intermittent voltage addition, a free intermittent time can be obtained by using a timer. In addition, it is possible to intermittently connect for a very short time by using a device for cutting current such as a thyristor. The application of voltage need not extend over the entire period of cultivation. In general, soil pests change depending on the growth stage, climate, and season of crops, and it is desirable to apply electricity in a timely manner to reduce costs.
[0012]
In order to convert direct current into alternating current having a free cycle, the electromagnetic relay can be opened and closed at a set time using a timer, and the anode and cathode of the direct current circuit incorporated in the relay can be alternately switched. The electrode material is preferably a copper or stainless steel plate that is not easily corroded, a net, or a spiral or hairy material such as cutting scraps, or an aluminum coating film that can be embedded easily and has a large surface area. .
[0013]
DETAILED DESCRIPTION OF THE INVENTION
Embodiments of the present invention will be described based on examples.
[0014]
[Example 1]
Behavior of nematode by applying voltage in petri dish 5 g of salt and 12 g of agar were heated and dissolved in 1 liter of tap water, and about 20 ml was poured into a 9 cm glass petri dish to prepare a flat plate. 200 microliters of suspended water containing about 20 stage II larvae of Meloidogyne incognita was dropped into the center of the petri dish. A copper electrode was embedded at a depth of 0.7 cm and a width of 1.5 cm so that both ends of the petri dish were parallel with a distance of 7 cm. Adjust the voltage to each level using a variable transformer (Sanyo Electric S-130-1), variable rectifier (Skynie BA-163), and tester (ETU-102A from Arrow Electronics Co., Ltd.). , AC electricity or DC electricity was applied. The behavior of the nematode was observed with a microscope for 5 minutes after energization. Some continued to observe the subsequent condition. The results are shown in Table 1.
[0015]
[Table 1]
As shown in the above table, the application of an alternating voltage of 100 V caused all individuals to die due to the fever of the agar, but had no lethal effect at 50 V or less. Moreover, the result which shows chemotaxis was obtained when direct current supply was carried out. The lethal effect of nematodes by direct current was judged to be due to electrochemical reactions.
[0016]
[Example 2]
Effect of voltage application on nematode parasitism 18 cm diameter clay pot filled with 850 ml of Kuroboku soil contaminated with sweet potato nematode (247 ± 80 heads / 20 g soil, 3 iterations, Bellman method), paralleled at a distance of 10 cm A copper plate having a width of 5 cm and a length of 10 cm was embedded. Five tomato seedlings (variety Tiny Tim) at the 3 leaf stage were planted between the electrodes and watered. Using a transformer (P-4106 and HT-1203, manufactured by Toyosumi Power Equipment Co., Ltd.), a 100V AC power source was subjected to continuous measurement of 7V and intermittent measurement of 14V. Moreover, the measured value of the voltage between the electrodes embedded in the soil by adjusting a dimming thyristor (PC-30 manufactured by Taiyo Denki Sangyo Co., Ltd.) was set to 15 V and energized. AC 14V intermittent energization was obtained by connecting a timer (H3CR manufactured by OMRON) in front of the transformer and turning it on and off every 0.05 seconds. One week later, liquid fertilizer diluted by a factor of 500 (fine powder hypoponex manufactured by Hyponex Japan) was sprayed. After 4 weeks, the tomatoes were dug up, and the soil pH was examined using a cat lobe parasitism, a foliage weight, and a pH meter (B-211, manufactured by Horiba, Ltd.). The degree of parasitism of the cat is 0 (no cat is recognized) to 1, 2, 3, 4 (the cat is found in almost 75% or more of the root system), and each stage has an intermediate position. The experiment was conducted in an unheated house. The average temperature during the experiment was 27.6 ° C. The average value of the results is shown in Table 2.
[0017]
[Table 2]
As described above, continuous or intermittent addition of AC electricity reduced the catob parasitic.
There was no significant difference in the degree of parasitism of the cat between 7V continuous and 14V intermittent with the same power consumption. Intermittent addition of Nekob parasitism using thyristors tended to be slightly lower. On the other hand, in all the energized plots, a decrease in the foliage weight was observed compared to the nematode control plot, suggesting that the application of voltage at high temperatures may inhibit the growth of crops.
[0018]
[Example 3]
Voltage application and inhibition of nematode parasitism and tomato spot disease A 850 ml of Kuroboku soil was packed in an unglazed pot having a diameter of 18 cm, and a copper plate having a width of 5 cm and a length of 10 cm was embedded so as to be parallel with a distance of 10 cm. Five two-leaf tomato seedlings (variety Tiny Tim) were planted between the electrodes. 10 ml of a suspension containing about 10,000 larvae of stage II larvae of sweet potato root-knot nematode was inoculated uniformly on the soil surface and watered. The resistance value between the electrodes immediately after irrigation was 250Ω. Using a variable transformer and variable rectifier, AC and DC were each converted to 6V and energized. The alternating current was turned on and off every 0.05 seconds using a timer and energized intermittently. One week later, liquid fertilizer diluted by a factor of 500 (fine powder hypoponex manufactured by Hyponex Japan) was sprayed. The onset of tomato spot disease was observed from around 1 week. Two weeks later, the number of leaf disease-causing leaves of the tomato first and second true leaves was examined. After the investigation of the number of diseased leaves, a 500-fold solution of bactericide (manzeb wettable powder) was sprayed. Soil pH was measured after 3 weeks. Six weeks later, the tomatoes were dug up to investigate the degree of cat parasitism, foliage weight, egg sac formation, and electrode condition. The degree of parasitism of the cat is 0 (no cat is recognized) to 1, 2, 3, 4 (a cat is found in almost 75% or more of the root system), and each stage has an intermediate position. The experiment was conducted in an unheated house. The average temperature during the experiment was 23.3 ° C. The average value of the results is shown in Table 3.
[0019]
[Table 3]
As described above, the intermittent AC voltage of 6 V or the application of a DC voltage suppressed the onset of tomato spot disease (Stemphyllium lycopersici) and the invasion of the root-knot nematode into the tomato root. At the same time, dark greening of tomato leaves was observed in the area where AC was added. On the other hand, the addition of direct current caused most strains to die, the stem and leaf weight also decreased, and the inhibition of tomato growth by electricity was significant. The cause was assumed to be due to a change in pH. In addition, inhibition of egg sac formation was not observed, and it was determined that it would not affect the next generation.
[0020]
[Example 4]
Adding upper limit voltage and nematode parasitic degree 18cm in diameter clay pot filled with 850ml of Kuroboku soil contaminated with sweet potato nematode (21 ± 6 heads / 20g soil, 3 repetitions, Bellman method) and paralleled at a distance of 10cm Thus, a copper plate having a width of 5 cm and a length of 10 cm was embedded. Five tomato seedlings (variety Tiny Tim) at the 4-leaf stage were planted between the electrodes and watered. A 100V AC power source was converted to 30V by a transformer (HT-3002 manufactured by Toyozumi Power Supply Co., Ltd.) and intermittently energized. In addition, using a dimming thyristor, the measured value of the interelectrode voltage was set to 15 V and 30 V, and the current was supplied. AC 30V intermittent energization connected a timer in front of the transformer and turned on and off every 0.05 seconds. One week later, liquid fertilizer diluted by a factor of 500 (fine powder hypoponex manufactured by Hyponex Japan) was sprayed. Changes in the ground temperature due to energization were measured during the day immediately after planting and in the next morning. Seven weeks later, the tomatoes were dug up to investigate the nematode parasitism, nematode density, and foliage weight. The degree of parasitism of the cat is 0 (no cat is recognized) to 1, 2, 3, 4 (a cat is found in almost 75% or more of the root system), and each stage has an intermediate position. The experiment was conducted in an unheated house. The average temperature during the experiment was 22.9 ° C. The average value of the results is shown in Table 4.
[0021]
[Table 4]
As described above, when a voltage of 15 V or higher was applied, an increase in the ground temperature was observed. When the measured voltage value of the thyristor was 30 V, the tomato died withering one day after the ground temperature exceeded 40 ° C. There wasn't. The 30V intermittent energization and the thyristor measured voltage value of 15V energization showed an increase in ground temperature of about 3 ° C., but did not affect the growth of tomatoes. In both cases, the catobacterial degree and the second generation nematode density were reduced by energization. However, in the thyristor 15V energized section, a decrease in tomato foliage weight was observed in spite of a decrease in the degree of parasitism of the cat.
[0022]
[Example 5]
Low frequency alternating current addition, Nekob parasitism and tomato wilt rate of 18cm diameter clay pot packed with 850ml of Kuroboku soil (63 ± 21 heads / 20g soil, 3 repetitions, Bellman method) contaminated with sweet potato root nematode, distance of 10cm A copper plate having a width of 5 cm and a length of 10 cm was embedded so as to be parallel to each other. Five three-leaf tomato seedlings (variety Tiny Tim) were planted between the electrodes, and about 15,000 larvae of stage II larvae were inoculated and watered. 100V AC power is converted to 0.7V DC using a variable transformer and rectifier (A20640j, manufactured by Toyo Keiki Co., Ltd.), and then incorporated into an electromagnetic relay (LY2 manufactured by OMRON Corporation) circuit for 1 hour, 1 day, 1 week Electricity was adjusted by adjusting the low-frequency alternating current in which the anode and the cathode were alternately switched every time. In addition, alternating 0.05-second intermittent energization for one week was switched on and off every 0.05 seconds by switching the anode and cathode every week and at the same time connecting a timer in front of the transformer. In the alternate energization for 1 week, the energization was interrupted every week, and the anode and the cathode were switched every time energization. As a control, a continuous energization zone of 0.7 V DC was provided. One week later, liquid fertilizer diluted by a factor of 500 (fine powder hypoponex manufactured by Hyponex Japan) was sprayed. Four weeks later, the number of true leaves was examined. Dwarf strains caused by withering disease were withdrawn from time to time, and the degree of parasitism in the cat was investigated. Eight weeks later, the tomatoes were dug up and the degree of cat parasitism and foliage were investigated. Further, the pH was measured with a test paper (universal pH 1-11 manufactured by Toyo Roshi Kaisha, Ltd.). The intermediate degree of each stage was set as four stages, from 0 (no recognized cat) to 1, 2, 3, 4 (approximately 75% or more of root system). The experiment was conducted in a warming house. The average temperature during the experiment was 16.8 ° C. The average value of the results is shown in Table 5.
[0023]
[Table 5]
In the above table, the nerobic parasite degree includes the dead strain and the foliage weight does not include the dead strain.
As described above, by converting low voltage direct current into alternating current having a long period, it has become possible to prevent the growth inhibition effect of crops that occurs when direct current is energized. At the same time, it was found that the occurrence of withering disease and the degree of parasitism of cat roots were reduced. Moreover, under the present conditions where the sweet potato nematode density was high and the cultivation temperature was low, reddish purple of the foliage, which is a phosphate deficiency symptom, was observed in all individuals.
[0024]
【The invention's effect】
The damage of soil pests is suppressed by continuously or intermittently supplying AC electricity with various periodicity at 30V or less that is safe for the human body and crops.
Claims (4)
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