JP3999384B2 - Cosmetics, quasi drugs, pharmaceuticals, food - Google Patents

Description

【００１７】

ＡとＢをそれぞれ計量し、７０℃まで加温し、ＢにＡを攪拌しつつ徐々に加えたのち、ゆっくり攪拌しつつ３０℃まで冷却した。
【００１８】
Ｂ−１６メラノーマ細胞試験
検体を所定の濃度になるように、ＥａｇｌｅｓＭＥＭ培地に加え、除菌フィルターでろ過後、牛胎児血清が１０％なるように加え、ｐＨを７．６±０．１になるように炭酸水素ナトリウムで調整し、シャーレに６ｍｌ分注し、Ｂ−１６メラノーマ細胞浮遊液（１×１０６ｃｅｌｌ／ｍｌ）を０．０５ｍｌ加え、５％ＣＯ ２、９５％ａｉｒの条件下で３７℃で３日間培養した。さらに、培地交換（上記の検体が入った１０％牛胎児血清含有ＥａｇｌｅｓＭＥＭ培地）を行い、３日間培養した。（このとき、細胞増殖を判定する）
細胞を剥離し、遠心分離して、細胞を集め、肉眼で白色度の判定を行った。
【００１９】
判定基準
白色度
ブランクと同程度 ±
わずかに白色化傾向 ＋
明らかに白色化傾向 ＋＋
強い白色化傾向 ＋＋＋
細胞増殖
ブランクの８０％以上 Ａ
ブランクの６０〜８０％ Ｂ
ブランクの３０〜６０％ Ｃ
ブランクの３０％以下 Ｄ
【００２０】
【表１】

【００２１】
動物での美白試験
有色モルモット（メス、３匹）の背部より両測腹部にわたる毛を電気バリカン及び電気シェーバーで刈毛、剃毛し、２cm×２cmに１％製造物−５０％エタノール溶液を０．０５mlづつ、毎日２回塗布した。対照として、５０％エタノールも同様に実施した。
２日目より紫外線（ＵＶ−Ｂ）を週３回、２週間、１回当たり、７５０mJ照射した。
２日目より２週間間隔で色差計でＬ*（明度）、M.I.（メラニンインデックス）を測定した。結果を表３に示す。
【００２２】
【表２】

【００２３】
結果は、Ｌ*、ＭＩとも照射期間中の２週間後までは対照と差がないが、２週間後から４週間後の黒化は対照に比較して製造例は大幅に抑制されている。
【００２４】
使用テスト
女性６名の顔面を左右に分け、一方を実施例、もう一方を比較例として毎日、１回以上使用してもらって、３月後、アンケートした。なお、比較例は実施例より製造例の各種の抽出物を水にかえたものである。（比較例１，２）

【００２５】
判定基準は以下のようでアンケートの結果をまとめたのが以下の表である。
実施例の方が非常によい ３
実施例の方がかなりよい ２
実施例の方がややよい １
差がない ０
比較例の方がややよい −１
比較例の方がかなりよい −２
比較例の方が非常によい −３
【００２６】

【００２７】
【効果】
鳳尾草(Pteris multifida Poir)の全草の溶媒抽出物は美白作用が非常に強く、これを配合した化粧料、医薬部外品、医薬品、食品は美白作用が強く有効である。[0001]
[Industrial application fields]
The present invention relates to a cosmetic, a quasi-drug, and a pharmaceutical containing a whitening material having a high whitening effect.
[0002]
[Prior art]
Lepidoptera is a plant belonging to the family Lamiaceae, and is called Pteris multifida Poir. It is a perennial herb with a plant height of 30 to 70 cm. The whole plant is used for medicinal purposes, and it is used for bacterial diarrhea, gastroenteritis, hepatitis, and the like. For external use, it is used as a hemostatic agent.
[0003]
On the other hand, various substances are known as whitening substances that can be used as raw materials for cosmetics, quasi-drugs, and pharmaceuticals, but synthetic products guarantee safety when applied to human skin for a long time. There is no use and is being restricted. On the other hand, many natural products have a weak whitening effect. However, it is a natural product from the viewpoint of safety against human skin, and has been eaten by humans for many years and guaranteed in terms of safety, and a substance having a whitening effect has been desired.
[0004]
[Problems to be solved by the invention]
An object of the present invention is to provide cosmetics, quasi-drugs, pharmaceuticals, and foods containing effective ingredients that are safe to apply to the skin and have a whitening effect.
[0005]
[Means for solving the problems]
In order to solve the above-mentioned problems, the present inventors screened and examined plants that have been used for food for many years and have been confirmed to be safe for the human body, and examined those that have utility value as cosmetics.
As a result, it was found that the whole herbal extract of Pteris multifida Poir is very effective as a raw material for cosmetics or as a raw material for quasi-drugs, pharmaceuticals, and foods.
[0006]
It has been found that the extraction method from lepidoptera should be extracted with water or a hydrophilic organic solvent alone or in combination. Examples of hydrophilic organic solvents include ethanol, methanol, butanol, ethylene glycol, diethylene glycol, triethylene glycol, higher polyethylene glycols, propylene glycol, dipropylene glycol, higher polypropylene glycols, 1,3-butylene glycol, Examples include butylene glycols such as 1,4-butylene glycol, glycerin, diglycerin, and higher polyglycerins.
[0007]
If necessary, remove the solvent from the plant extract and blend it into cosmetics, quasi drugs, pharmaceuticals, and foods. The blending amount varies depending on the use, but is generally about 0.0001 to 5%. This extract can be formulated into various dosage forms. For example, it can be in the form of lotions, emulsions, creams, ointments, packs, bathing agents. It can also be incorporated into foods and can be in the form of candy, chewing gum, tablets and the like.
[0008]
To formulate these cosmetics, quasi drugs, pharmaceutical dosage forms, natural animal and vegetable oils and fats such as olive oil, mink oil, castor oil, palm oil, beef tallow, evening primrose oil, coconut oil, castor oil, cocoa oil, Macadamia nut oil, etc .; wax such as jojoba oil, beeswax, lanolin, carnauba wax, candelilla wax, etc .; higher alcohols such as lauryl alcohol, stearyl alcohol, cetyl alcohol, oleyl alcohol; higher fatty acids such as lauric acid, palmitic acid, stearic acid Oleic acid, behenic acid, lanolin fatty acid, etc .; higher aliphatic hydrocarbons such as liquid paraffin, solid paraffin, squalane, petrolatum, ceresin, microcrystalline wax, etc .; synthetic ester oils such as butyl stearate, hexyl laurate, diisopropyl Dipate, diisopropyl sebacate, octyldodecyl myristate, isopropyl myristate, isopropyl palmitate isopropyl myristate, cetyl isooctanoate, neopentyl glycol dicaprate; silicone derivatives such as silicone oils such as methyl silicone and methyl phenyl silicone ]
Surfactants include anionic surfactants such as alkyl sulfates, fatty acid salts, alkyl phosphates, polyoxyethylene alkyl ether phosphates and sulfates, etc .; nonionic surfactants such as glycerin fatty acid esters , Polyoxyethylene sorbitan fatty acid ester, polyoxyethylene alkyl ether, sorbitan fatty acid ester, polyoxyethylene hydrogenated castor oil, polyglycerin fatty acid ester, etc .; In addition to phosphatidylglycerol, phosphatidylinositol and their lysates, phosphophatidic acid and its salts
Polyhydric alcohols such as ethylene glycol, diethylene glycol, triethylene glycol, higher polyethylene glycols, propylene glycol, dipropylene glycol, higher polypropylene glycols, 1,3-butylene glycol, 1,4-butylene glycol, etc. Butylene glycols, glycerol, diglycerol, higher polyglycerols, sugar alcohols such as sorbitol, mannitol, xylitol, maltitol, ethylene oxide of glycerol (hereinafter abbreviated as EO), propylene oxide (hereinafter abbreviated as PO) ) Adduct, EO of sugar alcohols, PO adduct, monosaccharides such as galactose, glucose, fructose and its EO, PO adduct, polysaccharide such as maltose, lactose and its EO, P Polyhydric alcohol such as adduct [0011]
Tocopherol, tocopherol acetate, vitamin C, allantoin, placental extract, elastin, arbutin, collagen, triclosan, trichlorocarban, dipotassium glycyrrhizinate, methylparaben, butylparaben as drugs
These materials are combined to obtain the necessary preparation.
However, the surfactant has one advantage even if it is not blended as described in JP-A-3-83909, JP-A-5-43416, and JP-A-6-9333, and it is not blended depending on the purpose. Of course, other raw materials are blended as necessary.
[0013]
Naturally, it can be blended in various forms as well in the case of food.
[0014]
【Example】
Examples which are actual utilization methods will be described below, but the present invention is not limited to these examples.
Production examples of the substances and extracts used in the present invention are shown below.
[0015]
Production Example 2 liters of 50% ethanol was added to 100 g of a dry product of whole plant of Pteris multifida Poir, and left for 5 days. After concentration under reduced pressure, it was lyophilized.
[0016]

[0017]

A and B were weighed and heated to 70 ° C., A was gradually added to B while stirring, and then cooled to 30 ° C. with slow stirring.
[0018]
B-16 melanoma cell test specimen is added to Eagles MEM medium to a predetermined concentration, filtered through a sterilization filter, and added to 10% fetal calf serum, resulting in a pH of 7.6 ± 0.1 So that it was adjusted with sodium hydrogen carbonate, dispensed 6 ml into a petri dish, added 0.05 ml of B-16 melanoma cell suspension (1 × 10 6 cells / ml), 37 ° C. under conditions of 5% CO 2 and 95% air. For 3 days. Further, the medium was exchanged (Eagles MEM medium containing 10% fetal bovine serum containing the above specimen) and cultured for 3 days. (At this time, cell proliferation is determined)
The cells were detached and centrifuged to collect the cells, and the degree of whiteness was determined with the naked eye.
[0019]
Judgment standard Same as whiteness blank ±
Slight whitening tendency +
Clearly whitening tendency ++
Strong whitening tendency +++
Over 80% of cell growth blank A
60-80% of blank B
30-60% of blank C
30% or less of blank D
[0020]
[Table 1]

[0021]
Whitening test in animals Colored guinea pigs (female, 3 animals) hair from the back to both abdominal areas were shaved and shaved with an electric clipper and electric shaver, and 1% product-50% ethanol solution was added to 2cm x 2cm. .05ml was applied twice daily. As a control, 50% ethanol was also used in the same manner.
From the second day, ultraviolet rays (UV-B) were irradiated 3 times a week for 2 weeks and 750 mJ per time.
From the second day, L * (brightness) and MI (melanin index) were measured with a color difference meter every two weeks. The results are shown in Table 3.
[0022]
[Table 2]

[0023]
As a result, both L * and MI were not different from the control until 2 weeks after the irradiation period, but the blackening after 2 weeks to 4 weeks was significantly suppressed in the production examples compared to the control.
[0024]
The test women's 6 female faces were divided into left and right, one was used as an example and the other was used as a comparative example. In the comparative example, various extracts of the production examples were replaced with water from the examples. (Comparative Examples 1 and 2)

[0025]
The criteria are as follows, and the results of the questionnaire are summarized in the following table.
The example is much better 3
The example is much better 2
Example is slightly better 1
No difference 0
Comparative example is slightly better -1
The comparative example is much better -2
The comparative example is much better -3
[0026]

[0027]
【effect】
The whole plant solvent extract of Pteris multifida Poir has a very strong whitening effect, and cosmetics, quasi-drugs, pharmaceuticals, and foods containing it have a strong and effective whitening effect.

Claims (1)

  Whitening cosmetics containing solvent extract of Kashio grass