JP4011945B2 - Topical skin preparation - Google Patents

Description

【００３５】
表１から明らかなように、本発明の実施例使用群ではいずれにおいても、保湿効果について非常に高い評価が得られた。これに対し、比較例使用群においても良好な評価が得られた場合もあるものの、その程度はそれぞれ対応する実施例使用群に比べて小さいものであった。
【００３６】
皮膚の老化症状改善評価の実使用試験は、パネラーとして、しわや皮膚弾性の低下といった皮膚の老化症状を顕著に呈する４０才〜６０才代の女性を１群２０名として用い、各群に実施例及び比較例をそれぞれブラインドにて１日２回使用させて行った。使用試験開始前及び終了後に皮膚の状態を観察し、しわ及び皮膚の弾性の改善状況について「改善」，「やや改善」，「変化なし」の３段階にて評価した。なお、しわの程度については写真撮影及びレプリカ採取により、皮膚の弾性についてはキュートメーターにより測定して評価した。結果は、各評価を得たパネラー数にて表２に示した。
【００３７】
【表２】

【００３８】
表２から明らかなように、本発明の実施例使用群ではいずれにおいても、しわ，皮膚弾性ともに全パネラーで症状の改善傾向が見られ、しわについては５０％以上、皮膚弾性については５５％以上のパネラーで明らかな改善を認めていた。これに対し、比較例使用群においても良好なしわ，皮膚弾性の改善が認められた場合もあるものの、その程度はそれぞれ対応する実施例使用群に比べて小さいものであった。
【００３９】
また、肌荒れ症状改善評価の実使用試験は、パネラーとして、肌荒れ症状を顕著に有する女性を１群２０名として用い、各群に実施例及び比較例をそれぞれブラインドにて１日２回使用させて行った。使用試験開始前及び終了後に皮膚の状態を観察し、肌荒れ症状の改善状況について、「改善」，「やや改善」，「変化なし」の３段階にて評価した。結果は、各評価を得たパネラー数にて表３に示した。
【００４０】
【表３】

【００４１】
表３から明らかなように、本発明の実施例使用群ではいずれにおいても、顕著な肌荒れの改善が認められ、使用試験終了後において皮膚の状態はほぼ良好な状態まで改善されていた。これに対し、比較例使用群においても良好な肌荒れの改善が認められた場合もあるものの、その程度はそれぞれ対応する実施例使用群に比べて小さいものであった。
【００４２】
つづいて色素沈着症状の改善効果は、顕著なシミ，ソバカス等の色素沈着症状を有する女性パネラー２０名を１群とし、各群に実施例及び比較例をそれぞれブラインドにて１日２回ずつ１カ月間使用させ、１カ月後の皮膚の色素沈着の状態を観察して使用前と比較して行った。色素沈着の状況は色素沈着を「認めない」，「やや認める」，「認める」の３段階にて評価した。結果は、各評価を得たパネラー数にて表４に示した。
【００４３】
【表４】

【００４４】
表４から、色素沈着症状についても、実施例使用群では良好な改善が認められ、使用試験終了時には、ほとんどのパネラーでわずかな色素沈着が見られる程度であった。これに対し、比較例使用群においても良好な色素沈着症状の改善が認められた場合もあるものの、その程度はそれぞれ対応する実施例使用群に比べて小さいものであった。
【００４５】
つぎに、実施例６〜実施例１１について、抗菌活性及び保存安定性の評価を行った。なお、各実施例において、エピロビウム・ラチフォリウム抽出物１〜同抽出物４を各抽出溶媒に、またエピロビウム・ラチフォリウム抽出物５をスクワランに代替して調製したものを比較例６〜比較例１１とし、同時に評価を行った。
【００４６】
抗菌活性は、試験菌として大腸菌（Escherichia coli），黄色ブドウ状球菌（Staphylococcus aureus），緑膿菌（Pseudomonas aeruginosa），アクネ菌（Pro pyonibacteriumacnes），黒カビ（Aspergillus niger ）及びふけ菌（Pityrosporum ovale ）を用い、これらをそれぞれ各実施例及び各比較例に１０⁶個／ｇ植菌し、細菌類は３７℃、真菌類は２５℃で培養し、２週間後の生菌数を計測して評価した。結果は、２週間後の生菌数が細菌については０個、真菌については１０³個／ｇ以下となっている場合を合格として、表５に示した。
【００４７】
保存安定性は、実施例及び比較例のそれぞれを２５℃で３カ月間保存し、変色，変臭，相分離や含有成分の析出といった外観の変化や変質の有無を観察して評価した。評価は「○；良好」，「△；やや悪い」，「×；悪い」として表し、表５にまとめて示した。
【００４８】
【表５】

【００４９】
本発明の実施例６〜実施例１１は、防腐剤を全く含有しないか、あるいは低濃度含有するのみであるが、表５において、いずれについても良好な抗菌活性と保存安定性が認められている。これに対し、比較例では特に緑膿菌，黒カビ及びふけ菌に対する抗菌活性が低く、保存安定性も良くなかった。
【００５０】
【発明の効果】
以上詳述したように、本発明により、保湿効果、皮膚の老化症状や炎症の防止，改善効果、皮膚の清浄化及び美白効果に優れ、且つ良好な抗菌活性を有し、安定性及び安全性に優れる皮膚外用剤を得ることができた。[0001]
BACKGROUND OF THE INVENTION
The present invention has an excellent moisturizing action and can exhibit both antibacterial activity and whitening action, and is useful for prevention and improvement of skin aging and inflammation, and prevention and improvement of pigmentation symptoms. And relates to an external preparation for skin having excellent stability and safety. More specifically, the present invention relates to an external preparation for skin containing an Epilobium latifolium extract.
[0002]
[Prior art]
The skin is exposed to various stresses existing in the environment such as heat, ultraviolet rays and various chemical substances. As a result, inflammatory reactions such as erythema and edema, allergic reactions, and blackening due to melanin formation occur in the skin, and as a result of exposure to such stress over a long period of time, skin wrinkles and blemishes occur, and elasticity decreases. It has been shown that aging symptoms progress. In addition, various resident bacteria exist on the skin, and they are abnormally grown by assimilating sebum and wastes, and the change in the flora is a cause of acne vulgaris and dandruff. It is also known that there is.
[0003]
Therefore, the search for active ingredients that prevent the above-mentioned inflammation and the like, prevent skin blackening and aging, and sterilize and clean the skin, and attempt to provide a skin external preparation containing them. Has been. For example, β-glycyrrhetinic acid, glycyrrhizic acid, salts and derivatives thereof, allantoin and derivatives thereof, azulene, ε-aminocaproic acid, hydrocortisone, etc. are used as anti-inflammatory agents. Antihistamines such as pheniramine are used, whitening agents such as ascorbic acid and derivatives thereof, kojic acid and derivatives thereof, hydroquinone derivatives such as arbutin, etc., skin sterilizers, cleansing agents such as hinokitiol, zinc pyrithione, photosensitizers, etc. Is used. In recent years, skin aging due to oxidative stress has been highlighted, and active oxygen species scavengers such as vitamin E and superoxide dismutase have also been added.
[0004]
However, many of the above-described components that have been used in the past are unstable to light, heat, etc., and their actions and effects are insufficient, so that sufficient effects can be obtained by blending them into an external preparation base. Needed to formulate a significant amount. In addition, there are few ingredients that can achieve all of the effects of moisturizing, preventing skin aging, skin whitening and skin sterilization, and cleaning. In order to exert these effects evenly, it is necessary to use many kinds of ingredients together. Depending on the components used in combination, the stability and action of each may be reduced.
[0005]
[Problems to be solved by the invention]
Therefore, in the present invention, the above-described problems are solved, and the external preparation for skin has the effects of moisturizing, preventing skin aging, whitening and sterilizing and cleaning the skin, and is excellent in stability and safety. The purpose was to provide.
[0006]
[Means for Solving the Problems]
Made various investigations to solve the above problems, Epirobiumu-Rachiforiumu (Epilobium latifolium) extract excellent moisturizing effect, anti-aging effects of the skin, there are whitening effect and antibacterial effect, further it to the skin external preparation base When added, the above-mentioned actions and effects were found not to be affected by the base component and excellent in storage stability, and the present invention was completed.
[0007]
That is, in the present invention, the Epirobiumu-Rachiforiumu (Epilobium latifolium) extract is contained in an external preparation base, it is an external preparation for skin.
[0008]
DETAILED DESCRIPTION OF THE INVENTION
Epirobiumu-Rachiforiumu used in obtaining the skin external preparation according to the present invention (Epilobium latifolium) is a vegetable of saffron (Onagraceae) family saffron (Epilobium) genus (Yanagiran (Chamaenerion) when classified in the genus also.) One, two or more selected from each part such as flowers, fruits, stems, leaves, roots, or the whole plant is used for extraction.
[0009]
These may be subjected to the extraction operation as they are, but considering the extraction efficiency, it is preferable to carry out the extraction after performing processing such as shredding, drying, and pulverization. Extraction is performed by immersing in an extraction solvent. In order to increase extraction efficiency, stirring may be performed or homogenization may be performed in an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but is suitably about 4 hours to 14 days. Moreover, the extraction method using a supercritical fluid or a subcritical fluid can also be taken.
[0010]
As an extraction solvent, water, lower alcohols such as methanol, ethanol, propanol, and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerin, diethyl ether, dipropyl ether, and the like Polar organic solvents such as ethers, esters such as ethyl acetate and butyl acetate, ketones such as acetone and ethyl methyl ketone, physiological saline, phosphate buffer, phosphate buffered saline, or petroleum ether , N-hexane, n-pentane, n-butane, n-octane, cyclohexane and other hydrocarbons, carbon tetrachloride, chloroform, dichloromethane, trichloroethylene, benzene, toluene and other nonpolar or low polarity solvents 1 Prefers seeds or two or more solvents It can be used for. Further, a supercritical fluid such as water, carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia, or a subcritical fluid may be used for extraction, and in this case, the above-mentioned solvent may be used as an entrainer.
[0011]
In addition, the extract can be added to the external preparation base as it is, but the concentrated and dried solid can be dissolved again in water or a polar solvent, or purified or fractionated by decolorization, deodorization, desalting, etc. You may use it after. For storage, it is preferable to freeze-dry after the purification treatment and dissolve in a solvent before use. Alternatively, they can be encapsulated in vesicles such as liposomes or microcapsules.
[0012]
In this invention, the said extract is contained in an external preparation base and is provided as a skin external preparation. The external preparation for skin according to the present invention can be provided in dosage forms such as lotions, emulsions, gels, creams, ointments and the like. Also, skin cosmetics such as lotions, emulsions, creams, packs, makeup base lotions, makeup base creams, emulsions, cream-like or ointment-type foundations, eye color, teak color makeup cosmetics, hand It can also be provided as body cosmetics such as creams, leg creams, and body lotions, body shampoos, detergents such as soaps, and hair cosmetics such as treatments and set agents.
[0013]
In addition to the extract according to the present invention, the external preparation for skin according to the present invention includes general oils, surfactants, moisturizers, ultraviolet absorbers, pigments, fragrances, preservatives, physiologically active ingredients and the like. Raw materials for pharmaceuticals and cosmetics can be contained. In addition, the blending amount of the extract according to the present invention into the external preparation for skin is appropriately about 0.0001 to 10.0% by weight, although it varies depending on the dosage form.
[0014]
【Example】
Further, the present invention will be described in detail by examples.
[0015]
Epirobium latifolium extract 1
After epirobium latifolium was crushed, 500 g was immersed in 1000 mL of a 50 vol% ethanol aqueous solution and allowed to stand at 25 ° C. for 7 days. Thereafter, insoluble materials were removed by filtration, the filtrate was concentrated under reduced pressure, and freeze-dried to obtain Epirobium / Ratifolium extract 1.
[0016]
Epirobium latifolium extract 2
The Epirobium / Ratifolium extract 1 prepared in Example 1 is dissolved in 10 mL of 50% by volume ethanol aqueous solution, and this is added to 100 mL of purified water and dissolved. To this, 80 g of soybean lecithin was added and suspended at 65 ° C., followed by sonication to prepare liposomes, which were collected by centrifugation to obtain Epirobium / Ratifolium extract 2.
[0017]
Epirobium latifolium extract 3
After pulverizing epirobium latifolium , 500 g is immersed in 2,000 mL of a 30% by volume 1,3-butylene glycol aqueous solution, stirred and extracted at 20 ° C. for 5 days, filtered to collect the filtrate, and epirobium latifolium is recovered. This was Extract 3.
[0018]
Epirobium latifolium extract 4
After pulverizing Epirobium / Latiforium , 500 g was homogenized in 2,000 mL of physiological saline, stirred as it was at 10 ° C. for a whole day and then centrifuged, and the supernatant was recovered and lyophilized to obtain Epirobium / Latiforium extract 4 It was.
[0019]
Epirobium latifolium extract 5
After pulverizing epirobium latifolium , 50 g was extracted at 40 ° C. with 15 MPa supercritical carbon dioxide fluid and 0.003% ethanol as an entrainer for 2 hours, and the resulting crude product was epirobium latifolium extract 5 It was.
[0020]
Example 1 Lotion for skin
(1) Ethanol 10.0 (% by weight) (2) Hydroxyethyl cellulose 1.0 (3) Epirobium / Ratifolium extract 1 0.1 (4) Purified water Mass production method with a total amount of 100: (1) to (4 ) To make uniform.
[0021]
Example 2 Emulsion for skin
(1) Stearic acid 0.2 (% by weight) (2) Cetanol 1.5 (3) Petrolatum 3.0 (4) Liquid paraffin 7.0 (5) Polyoxyethylene (10E.O.) monooleate 1.5 (6) Tocopherol acetate 0.5 (7) Glycerol 5.0 (8) Methyl paraoxybenzoate 0.1 (9) Triethanolamine 1.0 (10) Purified water Amount based on 100 (11 ) Epirobium latifolium extract 3 2.0
Production method: The oil phase components (1) to (6) are mixed, heated and uniformly dissolved, and kept at 70 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and heated to be uniform, and set to 70 ° C. The oil phase component is gradually added to the aqueous phase component while stirring to emulsify, and after cooling, (11) is added at 40 ° C.
[0022]
Example 3 Skin Gel
(1) Dipropylene glycol 10.0 (% by weight) (2) Carboxyvinyl polymer 0.5 (3) Potassium hydroxide 0.1 (4) Methyl paraoxybenzoate 0.1 (5) Purified water (6) Ascorbic acid phosphate magnesium salt 0.5 (7) Epirobium / Ratifolium extract 2 1.0
Production method: (2) is uniformly dissolved in (5), (4) is dissolved and added to (1), then (3) is added to increase the viscosity, and (6) and (7) are added. Added.
[0023]
Example 4 Skin Cream
(1) Beeswax 6.0 (% by weight) (2) Cetanol 5.0 (3) Reduced lanolin 8.0 (4) Squalane 27.5 (5) Glyceryl fatty acid ester 4.0 (6) Lipophilic glyceryl mono Stearate ester 2.0 (7) Polyoxyethylene (20E.O.) Sorbitan monolaurate ester 5.0 (8) Propylene glycol 5.0 (9) Methyl paraoxybenzoate 0.1 (10) Purified water Amount to be 100 (11) Retinol palmitate 0.5 (12) Epirobium / Ratifolium extract 4 3.0
Production method: The oil phase components (1) to (7) are mixed, dissolved, and heated to 75 ° C. On the other hand, the water phase components (8) to (10) are mixed and dissolved and heated to 75 ° C. Next, the oil phase component is added to the water phase component and pre-emulsified, and then uniformly emulsified with a homomixer. After cooling, (11) and (12) are added at 40 ° C.
[0024]
Example 5 Oil-in-water emulsion ointment
(1) White petrolatum 25.0 (% by weight) (2) Stearyl alcohol 25.0 (3) Glycerin 12.0 (4) Sodium lauryl sulfate 1.0 (5) Methyl parahydroxybenzoate 0.1 (6) Purification Amount based on total water of 100 (7) Retinoic acid 0.5 (8) Epirobium / Ratifolium extract 5 0.5
Production method: The oil phase components (1) to (4) are mixed, dissolved and made uniform, and heated to 75 ° C. On the other hand, the aqueous phase components (5) and (6) are mixed and dissolved, heated to 75 ° C., added with the oil phase component, emulsified, and cooled to 40 ° C. at (7), (8 ) And mix.
[0025]
Example 6 Lotion
(1) Ethanol 10.00 (% by weight) (2) 1,3-Butylene glycol 5.00 (3) Polyoxyethylene (20E.O.) sorbitan monolaurate 2.00 (4) Epirobium / Latifolium extract 5 0.01 (5) Fragrance 0.10 (6) Purified water Mass production method with a total amount of 100: Add (1) to (5) to (6) sequentially and mix and dissolve uniformly.
[0026]
Example 7 Makeup Base Cream
(1) Stearic acid 12.00 (% by weight) (2) Cetanol 2.00 (3) Glyceryl tri-2-ethylhexanoate 2.50 (4) Self-emulsifying glyceryl monostearate 2.00 (5) Propylene glycol 10.00 (6) Potassium hydroxide 0.30 (7) Methyl paraoxybenzoate 0.10 (8) Purified water Amount based on 100 (9) Titanium dioxide 1.00 (10) Bengala 0.10 (11) Yellow iron oxide 0.40 (12) Fragrance 0.10 (13) Epirobium / Ratifolium extract 2 3.00
Production method: The oil phase components (1) to (4) are mixed and heated to 75 ° C. to be uniform. On the other hand, the water phase components (5) to (8) are mixed, heated and dissolved at 75 ° C. to make uniform, and the pigments (9) to (11) are added to this and dispersed uniformly with a homomixer. . The oil phase component is added to the aqueous phase component, emulsified with a homomixer, cooled, and (12) and (13) are added and mixed at 40 ° C.
[0027]
Example 8 Liquid foundation
(1) Stearic acid 2.00 (wt%) (2) Squalane 5.00 (3) Octyldodecyl myristate 5.00 (4) Cetanol 1.00 (5) Polyglyceryl monoisopalmitate 9.00 (6 ) 1,3-butylene glycol 6.00 (7) Potassium hydroxide 0.10 (8) Methyl paraoxybenzoate 0.10 (9) Purified water Amount based on 100 (10) Titanium oxide 9.00 (11 ) Talc 7.40 (12) Bengala 0.50 (13) Yellow iron oxide 1.10 (14) Black iron oxide 0.10 (15) Fragrance 0.15 (16) Epirobium / Ratifolium extract 1 0.20
Production method: The oil phase components (1) to (5) are mixed and heated to 75 ° C. to be uniform. On the other hand, the water phase components (6) to (9) are mixed, heated and dissolved at 75 ° C. to make it uniform, and then the pigments (10) to (14) are added and dispersed uniformly with a homomixer. . The oil phase component is added to the water phase component, emulsified with a homomixer, cooled, and (15) and (16) are added and mixed at 40 ° C.
[0028]
Example 9 Shampoo
(1) Sodium alkyl ether sulfate 18.000 (% by weight) (2) Palm oil fatty acid diethanolamide 2.000 (3) Propylene glycol 2.000 (4) Epirobium latifolium extract 4 2.000 (5) Yellow 4 No. 0.001 (6) Fragrance 0.200 (7) Purified water Mass production method with a total amount of 100: (1) to (6) are sequentially added to (7) to make uniform.
[0029]
Example 10 Hair rinse
(1) Cetanol 3.000 (% by weight) (2) Stearyltrimethylammonium chloride 0.700 (3) Glycerol 3.000 (4) N-cocoyl-L-arginine ethyl ester-DL-pyrrolidone carboxylate 0.100 (5) Green No. 3 0.002 (6) Purified water Amount based on 100 (7) Fragrance 0.100 (8) Epirobium / Ratifolium extract 1 3.000
Production method: The aqueous phase components (3) to (6) are mixed, dissolved, and heated to 70 ° C. On the other hand, the oil phase components (1) and (2) are mixed and dissolved, and heated to 70 ° C. The oil phase is gradually added to the aqueous phase with stirring, pre-emulsified, and homogenized with a homomixer, cooled, and (7) and (8) are added and mixed at 40 ° C.
[0030]
Example 11 Liquid Body Cleaning Material
(1) N-lauroyl-L-glutamic acid triethanolamine (30.0 wt% aqueous solution) 20.0 (wt%) (2) N-laurylmethyltaurine sodium (30.0 wt% aqueous solution) 10.0 (3 ) Triethanolamine laurate 10.0 (4) Triethanolamine myristate 10.0 (5) Laurylimidazolinium betaine 5.0 (6) Lauroyldiethanolamide 5.0 (7) Propylene glycol 7.0 (8 ) Purified water Amount based on 100 (9) Fragrance 0.1 (10) Epirobium / Ratifolium extract 1 1.5
Production method: (1) to (10) are sequentially added and mixed to make uniform.
[0031]
Example 12 Emollient cream (water-in-oil type)
(1) Liquid paraffin 30.0 (wt%) (2) Microcrystalline wax 2.0 (3) Petrolatum 5.0 (4) Diglyceryl dioleate 5.0 (5) Sodium L-glutamate 1.6 (6) L-serine 0.4 (7) Propylene glycol 3.0 (8) Methyl paraoxybenzoate 0.1 (9) Epirobium / latiforium extract 3 0.2 (10) Purified water Amount based on 100 (11) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (10) to 50 ° C, and gradually add to (4) heated to 50 ° C with stirring. This was mixed in advance and uniformly dispersed in (1) to (3), which was heated and dissolved at 70 ° C, and (7) to (9) were dissolved in the remainder of (10) and heated to 70 ° C. Add with stirring and emulsify with homomixer. After cooling, add and mix (11) at 40 ° C.
[0032]
Among the above Examples, Examples 1 to 5 were subjected to an actual use test for 6 months in order to evaluate the moisturizing effect, the skin aging preventing effect, the skin roughening improving effect and the whitening effect. At that time, in each Example, Comparative Examples 1 to 5 were prepared by substituting Epirobium / Latiforium Extract 1 to 4 for each extraction solvent and replacing Epirobium / Ratiforium Extract 5 with squalane. At the same time, the evaluation was performed.
[0033]
First of all, the actual use test for evaluating the moisturizing effect used 20 women in their 20s to 50s who complained of dry skin as a panel as 20 people per group. It was made to use once. The evaluation was made in three stages: moist feeling “very”, “somewhat” and “not”. The results are shown in Table 1 as the number of panelists that gave each evaluation.
[0034]
[Table 1]

[0035]
As is clear from Table 1, in each of the examples using groups of the present invention, a very high evaluation was obtained regarding the moisturizing effect. On the other hand, although a favorable evaluation was sometimes obtained in the comparative example use group, the degree thereof was smaller than the corresponding example use group.
[0036]
The actual use test of skin aging symptom improvement evaluation was carried out in each group using as a panel a group of 20 women in their 40s to 60s who had marked skin aging symptoms such as wrinkles and reduced skin elasticity. Each of the examples and comparative examples was performed twice a day using blinds. The condition of the skin was observed before and after the use test was started, and the improvement status of wrinkles and skin elasticity was evaluated in three stages: “improved”, “somewhat improved”, and “no change”. The degree of wrinkle was evaluated by measuring the elasticity of the skin with a cute meter by taking a picture and taking a replica. The results are shown in Table 2 in terms of the number of panelists that obtained each evaluation.
[0037]
[Table 2]

[0038]
As is clear from Table 2, in all the groups using the examples of the present invention, both the wrinkles and the skin elasticity showed a tendency to improve the symptoms in all the panelists. The wrinkles were 50% or more, and the skin elasticity was 55% or more. Panelists acknowledged a clear improvement. On the other hand, although good wrinkles and improvement in skin elasticity were sometimes observed in the comparative example use group, the degree was smaller than the corresponding example use group.
[0039]
Moreover, the actual use test of rough skin symptom improvement evaluation uses the woman who has a rough skin symptom remarkably as a panel as 20 persons, and makes each group use an example and a comparative example twice a day blindly, respectively. went. The skin condition was observed before and after the start of the use test, and the improvement status of rough skin symptoms was evaluated in three stages: “improved”, “somewhat improved”, and “no change”. The results are shown in Table 3 in terms of the number of panelists that obtained each evaluation.
[0040]
[Table 3]

[0041]
As is clear from Table 3, in any of the examples using groups of the present invention, remarkable improvement in rough skin was observed, and after completion of the use test, the skin condition was improved to a substantially good condition. On the other hand, although the improvement of rough skin may be recognized also in the comparative example use group, the degree was small compared with the corresponding Example use group.
[0042]
Subsequently, the effect of improving pigmentation symptoms was defined as one group of 20 female panelists having pigmentation symptoms such as remarkable spots and buckwheat. Each example and comparative example was blinded twice a day for each group. It was used for one month, and the state of skin pigmentation after one month was observed and compared with that before use. The status of pigmentation was evaluated in three levels: “not recognized”, “slightly recognized”, and “accepted”. The results are shown in Table 4 in terms of the number of panelists that obtained each evaluation.
[0043]
[Table 4]

[0044]
From Table 4, also regarding the pigmentation symptom, good improvement was observed in the group using the examples, and at the end of the use test, slight pigmentation was observed in most panelists. On the other hand, although the improvement of the pigmentation symptom may be recognized even in the comparative example use group, the degree is smaller than the corresponding example use group.
[0045]
Next, for Examples 6 to 11, antibacterial activity and storage stability were evaluated. In addition, in each Example, what was prepared by substituting Epirobium latifolium extract 1 to 4 for each extraction solvent and substituting Epirobium latifolium extract 5 for squalane was set as Comparative Examples 6 to 11. Evaluation was performed at the same time.
[0046]
Antibacterial activity, E. (Escherichia coli) as the test bacteria, Staphylococcus aureus (Staphylococcus aureus), Pseudomonas aeruginosa (Pseudomonas aeruginosa), Propionibacterium acnes (Pro pyonibacteriumacnes), a black mold (Aspergillus niger) and dandruff fungus (Pityrosporum ovale) 10 ⁶ cells / g were inoculated in each example and each comparative example, and the bacteria were cultured at 37 ° C. and the fungi were cultured at 25 ° C., and the number of viable bacteria after 2 weeks was measured and evaluated. . The results are shown in Table 5 when the number of viable bacteria after 2 weeks is 0 for bacteria and 10 ³ / g or less for fungi as a pass.
[0047]
The storage stability was evaluated by storing each of the Examples and Comparative Examples at 25 ° C. for 3 months, and observing the appearance change and alteration such as discoloration, odor change, phase separation and precipitation of contained components. The evaluation was expressed as “◯; good”, “Δ; somewhat bad”, “×; bad”, and summarized in Table 5.
[0048]
[Table 5]

[0049]
Examples 6 to 11 of the present invention contain no preservative or contain only a low concentration, but in Table 5, good antibacterial activity and storage stability are recognized for all. . On the other hand, in the comparative example, antibacterial activity was particularly low against Pseudomonas aeruginosa, black mold and dandruff, and storage stability was not good.
[0050]
【The invention's effect】
As described above in detail, according to the present invention, it has excellent moisturizing effect, prevention of skin aging and inflammation, improvement effect, skin cleansing and whitening effect, good antibacterial activity, stability and safety It was possible to obtain an external preparation for skin having excellent resistance.

Claims (1)

Skin external preparation comprising a Epirobiumu-Rachiforiumu (Epilobium latifolium) extract.