JP4077950B2 - Container with lid - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a vessel with a lid that can be suitably used as a multi-cell type cell culture vessel for cell and tissue culture, particularly for cloning of hybridomas for cultured cell gene mutation tests and monoclonal antibody production.
[0002]
[Prior art]
Cell culture containers including multi-tuft cell culture containers are generally used for in vitro culture of cells. Particularly, multi-tuft cell culture containers are usually 6, 12, 24, and 48. And 96 holes called so-called culture chambers.
[0003]
With regard to the specific structure of the multi-cell type cell culture vessel, for example, in addition to Japanese Patent Publication No. 62-60075, U.S. Pat. Nos. 4,349,632, 4,038,149, 4,012,288, 4010078, and 3,597,326 And No. 3107204.
[0004]
Among these, the container with a lid disclosed in Japanese Patent Publication No. Sho 62-60075 covers the container body 4 and the opening surface of the container body 4 as is apparent from the overall perspective view shown in FIG. The lid-covered body 2 constitutes the entire lidded container 1.
[0005]
In this case, the container body 4 is formed with a plurality of independent holes 3 called culture chambers, and the cells can be cultured in these holes 3. The cultured cells can also maintain an isolated state for each hole 3. Therefore, when the container 1 is used, various culture-related tests and analyzes such as observation of the reaction of cultured cells to a specific substance (proliferation and morphological changes) can be suitably performed. Become.
[0006]
However, when culturing using the same vessel 1, each sample should normally not be mixed between the holes 3, so that the sample or the like in each hole 3 can be separated. Notes had to be made individually.
[0007]
On the other hand, FIG. 4 shows a container with a lid called a well-known Terasaki plate used for tissue blood type determination such as cell culture of small volume, HLA typing, lymphocyte classification test, complement binding reaction and the like. It is a whole perspective view.
[0008]
The entire container 5 with a lid, called a terasaki plate, is similar to the multi-bottle type container 1 shown in FIG. 3 and includes a container body 6 and a lid body 9 that covers and covers the opening surface of the container body 6. It consists of The container 5 has a structure having a plurality of holes 8 recessed in the bottom plate part 7.
[0009]
[Problems to be solved by the invention]
By the way, in the case of the lidded container 1 shown in FIG. 3, the same sample may be dispensed in all the holes 3 in the test and analysis work related to cell culture. Thus, it is not necessary to pour a liquid sample into each hole 3 independently, and even if a sample is mixed between the holes 3, there is no problem as long as the desired amount per each hole 3 can be dispensed. is there. Specifically, the same cell suspension is dispensed into all of the holes 3, and after culturing for a certain period of time, the cultured cells are subjected to observation of the holes 3 and counting or isolation of colonies of the formed cells. There are experiments such as gene mutation tests and screening of hybridomas for monoclonal antibody production. In such an experiment, the operation of dispensing a sample or the like independently for each hole 3 is very complicated and time consuming. The container (multi-batch culture container) 1 having 96 holes 3 can be efficiently dispensed by using currently or commercially available 8 or 12 pipettes. It will be a very complicated task. If the number of treatments becomes large, the work efficiency can be improved by using a known automatic dispenser, etc., but the automatic dispenser itself is very expensive and is used in a small-scale experiment stage. There was an inconvenience that the use of was not economically paid.
[0010]
In addition, in the case of the lidded container (Terasaki plate) 5 shown in FIG. 4, each hole 8 provided in the container main body 6 generally has a small capacity, so that the amount of liquid that can be held is small, and from the opening of the hole 8 to the bottom In the meantime, there is a problem that it is not suitable for a cultured cell gene mutation test and the like because it is substantially concave or mortar and is not suitable for fine microscopic observation.
[0011]
In the present invention, in view of the above-described problems found in the prior art, after pouring a liquid sample into the surrounding space of the container body, a predetermined amount of the liquid sample is removed by simply sucking out an excess liquid sample with a suction nozzle. A container with a lid that can be dispensed into the hole, speeding up the process, and can be used for experiments such as cultured cell gene mutation tests and hybridoma screening for monoclonal antibody production. The purpose is to provide.
[0012]
[Means for Solving the Problems]
The present invention has been made in order to achieve the above-mentioned object, and has the structural feature of having an enclosed space defined by standing a dam plate around a bottom plate having a required thickness. A plurality of holes that can individually hold a liquid sample in a fixed amount on the bottom plate of the container body. And at least one liquid reservoir hole useful for adjusting the liquid level by sucking and removing the surplus portion of the liquid sample poured into the surrounding space of the container main body with a suction nozzle. The respective opening portions are positioned on the flat upper surface of the bottom plate portion and individually recessed.
[0013]
In this case, the hole portion has a circular planar shape with a taper that gradually increases in diameter from the bottom surface portion toward the opening portion, and has a depth of 2 to 7 mm. It is preferable to have a capacity of In addition, it is preferable that the holes are arranged in the row direction and the column direction, and are spaced apart so that the distances between adjacent ones in the row or column direction are equal.
[0014]
Furthermore, a series of holes located in the outermost periphery and in proximity to the dam plate portion are recessed in an arrangement relationship in which each outer edge reaches the inner surface side of the dam plate portion, It is preferable that an arc-shaped concave curved surface that goes directly up along the diameter-expanded shape of each hole in the reached part is cut and continued on the inner side surface. In addition, it is preferable to form the said container main body and a cover body with a transparent glass material or a transparent synthetic resin material.
[0015]
DETAILED DESCRIPTION OF THE INVENTION
FIG. 1 is an overall perspective view showing an example of a container with a lid according to the present invention in an open state, around a bottom plate portion 13 having a required plate thickness and at least an upper surface 14 being flat. The container body 12 includes an enclosure space 20 defined by standing the dam plate portion 17, and a lid body 21 that covers and covers the opening surface 12 a of the container body 12. .
[0016]
Among these, the container main body 12 has, for example, a bottom plate portion 13 having a rectangular planar shape with a short side of 70 to 90 mm, a long side of 120 to 130 mm, and a plate thickness of 3 to 8 mm, and an upper surface of the bottom plate portion 13. 14 and a dam plate portion 17 having a height of 4 to 6 mm so that a required amount of liquid sample L poured from a test tube 25 can be accommodated as shown in FIG. Is formed. In addition to the illustrated example, the container body 12 can be appropriately formed under appropriate dimensions determined in relation to the required number of holes 15.
[0017]
Moreover, as shown in FIG. 2 (c), the bottom plate portion 13 has a hole portion 15 having a plurality of bottom surface portions 15 b that can individually hold a predetermined amount of the liquid sample L, and an enclosure space 20 of the container body 12. the excess was removed by suction with the suction nozzle 26 over the surface and, for example, one liquid reservoir hole 16 to be utilized is flat when adjusting the liquid level of the liquid sample L was poured within 14, each opening 15a, 16a is positioned and recessed individually.
[0018]
Among these, each hole portion 15 has a circular planar shape with a taper that gradually increases in diameter from the bottom surface portion 15b toward the opening portion 15a, and has a depth of 2 to 7 mm. It is desirable to make a recess under the same capacity that presents a substantially frustoconical shape inverted.
[0019]
In particular, in the case of cell culture such as a cultured cell gene mutation test, it is sufficient to secure a capacity of about 40 to 3500 μl as the internal space of each hole 15, so the inner diameter of the hole 15 is in the range of 5 to 25 mm. Can be set as appropriate. In addition, it is desirable that the bottom surface portion 15b of the hole portion 15 has a circular planar shape because the bottom surface portion 15b of the hole portion 15 has an angular shape such as a square when isolating colonies formed by cells. This is because the shape makes it difficult to collect colonies or the like in the corner.
[0020]
The reason why the depth of each hole 15 is 2 to 7 mm is that if the depth from the bottom surface 15 b to the opening 15 a is shallower than 2 mm, the internal space volume of the hole 15 becomes small and can be retained. This is because there is a disadvantage in conducting the cultured cell gene mutation test. Moreover, when the depth of the hole 15 is deeper than 7 mm, there is a possibility that it may hinder observation and isolation of colonies formed by cells in cell culture such as a cultured cell gene mutation test which is the main use. Because.
[0021]
Furthermore, the individual hole portions 15 are recessed so that a total of 96 holes can be arranged on the bottom plate portion 13 of the container body 12 by, for example, twelve rows or column directions and eight columns or row directions. For example, they are arranged in the row direction and the column direction, and are spaced apart so that the distances between adjacent ones in the row or column direction are equal. In addition, in this case, although not shown, for example, the upper end surface 17a of the dam plate portion 17 of the container main body 12 is used, and the arithmetic numbers “1” to “1” along the row direction of the hole portions 15 are used. If appropriate symbols indicating the position coordinates are separately displayed in the row direction and the column direction, such as “12” is displayed along the column direction and alphabets “A” to “H” are respectively displayed. This is preferable because the position of the hole 15 can be quickly identified.
[0022]
In addition, as shown in FIG. 1, a series of hole portions 15 located on the outermost periphery in the bottom plate portion 13 are recessed under an arrangement relationship in which each outer edge reaches the inner surface 18 side of the dam plate portion 17. In addition, if the inner side surface 18 is formed with an arc-shaped concave curved surface 18a that extends directly along the diameter-expanding shape of each hole 15 at the portion that reaches the inner side surface 18, the container body This is preferable because the supply amount of the liquid sample L to be poured into 12 can be made the minimum necessary amount.
[0023]
Furthermore, as shown in FIG. 2B, the liquid reservoir hole 16 prepared for inserting the suction nozzle 26 for sucking the liquid sample L has an opening 16 a that is different from the opening 15 a of the hole 15. Similarly, as long as it is located on the upper surface 14 of the bottom plate portion 13, the depth may be smaller than the depth of the hole portion 15 and may hold a capacity smaller than the capacity of the hole portion 15. Further, the arrangement position and the number of the liquid reservoir holes 16 are not particularly limited as long as the hole plate 15 is not provided with the hole 15, and may be provided at one or a plurality of appropriate sides. It is preferable to leave.
[0024]
Furthermore, in the container body 12, at least one of the front and rear sides or the left and right sides, in the embodiment shown in FIG. Although an example in which the lid body 21 is more easily covered by using the slopes 19a and 19a formed by inclining forward 19 as a guide surface is shown, the present invention is not limited to this. For example, it can be appropriately selected as desired.
[0025]
The container body 12 and the lid body 21 are preferably formed using a transparent resin material such as polystyrene or a transparent glass material.
[0026]
Since the present invention is configured in this way, when its use is that the liquid specimen L can be dispensed into each hole 15. Through the process of FIG. 2 (a) ~ (c) become.
[0027]
That is, a person engaged in various tests and experiments puts a liquid sample L such as an adjusted cell suspension in a predetermined amount in the test tube 25 in advance in the container main body 12 and the liquid level on the bottom plate portion 13. By simply pouring the liquid sample so as to be higher than the surface 14, a liquid sample is obtained at an appropriate liquid level in the surrounding space 20 including the inside of the hole 15 and the inside of the liquid reservoir hole 16 as shown in FIG. L can be satisfied.
[0028]
After the injection of the liquid sample L into the container body 12 is finished, the surplus portion is removed by inserting the suction nozzle 26 into the liquid reservoir hole 16 and sucking the liquid sample L as shown in FIG. It can be eliminated to lower the liquid level.
[0029]
Moreover, the opening 16a of the liquid reservoir hole 16, so located on the surface 14 of the opening 15a as well as the bottom plate portion 13 of the hole 15, by continuing the suction 引除 being worked by the suction nozzle 26 Eventually, the liquid level of the liquid sample L can be lowered to the upper surface 14 of the bottom plate portion 13 as shown in FIG. Further, by continuing the suction 引除 removed by work after asked liquid sample L in the liquid reservoir hole 16 side by the suction nozzle 26 relative to the liquid reservoir hole 16 by tilting the container main body 12 as needed, each hole The liquid sample L can be left and removed only in the part 15.
[0030]
For this reason, the liquid sample L poured into the container main body 12 only remains in each hole portion 15, and all of the excess liquid sample L can be excluded from the container main body 12. That is, all the hole portions 15 can be obtained by simply pouring the liquid sample L into the surrounding space 20 of the container main body 12 without performing an individual dispensing operation of individually injecting the liquid sample L into each hole portion 15. A predetermined amount of the liquid sample L corresponding to the volume can be automatically held in the inside.
[0031]
【The invention's effect】
As described above, according to the present invention, the bottom plate portion has a plurality of holes capable of individually holding a fixed amount of liquid sample and at least one liquid reservoir hole , each having an opening on a flat upper surface. Since each part is recessed by being located, the liquid sample in the liquid reservoir hole can be removed by suction with the suction nozzle without going through the individual dispensing operation of individually injecting the liquid sample into each hole. Thus, a predetermined amount of liquid sample corresponding to the volume can be automatically held in all the holes, which can greatly contribute to the improvement of working efficiency during experiments or tests.
[0032]
In addition, when the depth is 2 to 7 mm and each hole is recessed under the same capacity that exhibits a substantially frustoconical shape that is inverted, the cultured cell gene mutation test, etc. In addition to being able to easily collect colonies formed by cells, it is possible to secure a sufficient cell culture medium if necessary, as well as for main applications. Observation and isolation of colonies formed by cells in cell culture such as cultured cell gene mutation test can be performed smoothly.
[0033]
Further, when the individual hole portions are arranged in the bottom plate portion in the row direction and the column direction and are arranged so as to be spaced apart from each other in the row or column direction, the main holes Counting of colonies formed by cells in a cultured cell gene mutation test or the like, which is an application, can be facilitated.
[0034]
Furthermore, when a concave curved surface that is directly above the enlarged diameter shape of each hole is formed on the inner surface of the dam plate, the supply amount of the liquid sample to be poured into the container main body is minimized. Therefore, workability can be improved.
[0035]
In addition, when a slope is formed on the outer surface of the dam plate portion of the container main body, it can be used as a guide surface when the lid is applied. It is possible to make it function effectively, for example.
[Brief description of the drawings]
FIG. 1 is an overall perspective view showing an example of a lidded container according to the present invention in an opened state.
FIG. 2 shows a work procedure when a liquid sample is dispensed into a lidded container according to the present invention, taking as an example a vertical cross-sectional view in FIG. Explanatory drawing shown as (c).
FIG. 3 is an overall perspective view showing a conventional example of a multi-cell type cell culture container in an opened state.
FIG. 4 is an overall perspective view showing a conventional example of a container with a lid generally called a terasaki plate under an open state.
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 11 Container 12 with a lid Main body 12a Opening surface 13 Bottom plate part 14 Upper surface 15 Hole part 15a Opening part 15b Bottom part 16 Reservoir hole part 16a Opening part 17 Dam plate part 17a Upper end surface 18 Inner side surface 18a Concave surface 19 Outer side surface 19a Slope 20 Surrounding space 21 Lid 25 Test tube 26 Suction nozzle L Liquid sample

Claims (5)

A container main body having a surrounding space defined by standing a dam plate around a bottom plate having a required plate thickness, and a lid that covers and covers the opening surface of the container main body The bottom plate portion of the container body sucks the excess portion of the liquid sample poured into the surrounding space of the container body, and a plurality of holes that can individually hold the liquid sample in a fixed amount. At least one liquid reservoir hole that is useful for adjusting the liquid level by sucking and removing with a nozzle is individually recessed with each opening positioned on the flat upper surface of the bottom plate. A container with a lid.  The hole portion has a circular planar shape with a taper that gradually increases in diameter from the bottom surface portion toward the opening, and has a depth of 2 to 7 mm, and each has the same capacity. The container with a lid according to claim 1, wherein the container has a lid.  3. Each of the hole portions is aligned in the row direction and the column direction, and is arranged so as to be spaced apart from each other so that the mutual distances adjacent in the row or column direction are equal. Container with lid.  A series of hole portions located on the outermost periphery and close to the dam plate portion are recessed under the arrangement relationship in which each outer edge reaches the inner surface side of the dam plate portion, and reaches the inner surface. The lid according to any one of claims 1 to 3, wherein an arc-shaped concave curved surface that is directly above the enlarged diameter shape of each hole portion of the part is formed by cutting the inner side surface continuously. container. The container with a lid according to any one of claims 1 to 4 , wherein the container body and the lid are formed of a transparent glass material or a transparent synthetic resin material.

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