JP4087353B2 - Liquid chromatograph, liquid chromatograph control device, and liquid chromatograph control program - Google Patents

Description

  The present invention relates to a liquid chromatograph, a liquid chromatograph control device, and a liquid chromatograph control program.

  As described in Patent Document 1, in liquid chromatography, a sample dissolved in a mobile phase, which is an eluent mixed with a plurality of solvents, is passed through a stationary phase packed in a column. At this time, each component in the sample introduced into the column has different time to pass through the column due to the interaction with the stationary phase and the affinity with the mobile phase. Are separated. A substance having a strong interaction with the stationary phase or a weak affinity with the mobile phase stays in the column for a long time and is eluted slowly from the column.

JP 2003-240765 A

  The liquid chromatograph includes a normal mode in which the sample eluted from the column is separated into a plurality of containers over the entire elution time regardless of its concentration, and a period during which the concentration of the sample eluted from the column exceeds a predetermined value. There is one that can selectively execute the peak mode in which only the container is sorted. However, in the peak mode, if there is a failure of the detector due to a dirty flow cell or the like, a failure of the multi-way valve attached to the fraction collector, the sample to be dispensed is discarded.

  An object of the present invention is to provide a liquid chromatograph, a liquid chromatograph control device, and a liquid chromatograph control capable of preventing high-concentration components eluted from a column from being discarded due to a failure of a detector or a multi-way valve. Is to provide a program.

Means and effects for solving the problems

  The liquid chromatograph of the present invention includes a column packed with a stationary phase, a pump for supplying an eluent to the column, a detector for detecting a concentration of a component contained in a sample eluted from the column, and the column A fraction collector for separating the sample supplied to the first port of the multi-way valve after being eluted into the plurality of containers through the second port of the multi-way valve, and from the third port of the multi-way valve A first liquid sensor for detecting whether or not a liquid has flowed out, and between the first port and the second port of the multi-way valve when the concentration detection level by the detector is higher than a predetermined level. And the multi-way valve is controlled so that the first port and the third port of the multi-way valve communicate with each other when the concentration detection level by the detector is lower than a predetermined level. Control And when the concentration detection level by the detector is higher than a predetermined level and the first liquid sensor detects that the liquid flows out from the third port, the pump is suspended. First pump stop control means.

  The liquid chromatograph control device of the present invention includes a column filled with a stationary phase, a pump for supplying an eluent to the column, a detector for detecting the concentration of a component contained in a sample eluted from the column, A fraction collector for separating the sample that has been eluted from the column and then supplied to the first port of the multi-way valve into a plurality of containers via the second port of the multi-way valve; and a third of the multi-way valve A liquid chromatograph control device comprising a first liquid sensor for detecting whether or not liquid flows out from a port, wherein the multi-way valve is used when a concentration detection level by the detector is higher than a predetermined level. The first port of the multi-way valve and the third port when the first port and the second port of the multi-way valve communicate with each other and the concentration detection level by the detector is lower than a predetermined level. A valve control means for controlling the multi-way valve so as to communicate with the port; a concentration detection level by the detector is higher than a predetermined level; and the liquid from the third port is detected by the first liquid sensor. And a first pump stop control means for temporarily stopping the pump when it is detected that there is an outflow.

  According to the present invention, it is possible to prevent the high concentration component eluted from the column from being discarded through the third port of the multi-way valve when the multi-way valve has failed.

  The liquid chromatograph of the present invention may further include a second liquid sensor that detects whether or not there is a liquid outflow from the second port of the multi-way valve. At this time, the first pump stop control means confirms that the concentration detection level by the detector is higher than a predetermined level, and the first liquid sensor has an outflow of liquid from the third port. When it is detected, or when both the first and second sensors detect that there is no liquid outflow, the pump is temporarily stopped.

  As a result, a failure of the multi-way valve can be detected more reliably, so that the high concentration component eluted from the column is further discarded through the third port of the multi-way valve.

  In another aspect, the liquid chromatograph of the present invention includes a column packed with a stationary phase, a pump for supplying an eluent to the column, and detection for detecting the concentration of a component contained in the sample eluted from the column. A fraction collector for separating the sample that has been eluted from the column and then supplied to the first port of the multi-way valve into a plurality of containers via the second port of the multi-way valve, and the first of the multi-way valve A first liquid sensor that detects whether or not there is a liquid outflow from the three ports, and the first port and the second of the multi-way valve when the concentration detection level by the detector is higher than a predetermined level. The first port and the third port of the multi-way valve communicate with each other when the concentration detection level by the detector is lower than a predetermined level. Many Valve control means for controlling the valve, signal supply means for supplying a signal having a level higher than the predetermined level to the detector immediately after the start of driving of the pump, and the first liquid sensor from the third port And a first pump stop control means for temporarily stopping the pump when it is detected that there is a liquid outflow.

  In this aspect, the liquid chromatograph control device of the present invention detects a concentration of a component contained in a sample eluted from the column, a column filled with a stationary phase, a pump for supplying an eluent to the column. A detector, a fraction collector for separating the sample that has been eluted from the column and then supplied to the first port of the multi-way valve into a plurality of containers via the second port of the multi-way valve, and the multi-way valve A liquid chromatograph control device including a first liquid sensor for detecting whether or not a liquid flows out of the third port, wherein the concentration detection level by the detector is higher than a predetermined level. When the first port and the second port of the multi-way valve communicate with each other and the concentration detection level by the detector is lower than a predetermined level, the first one of the multi-way valve A valve control means for controlling the multi-way valve so that the communication port communicates with the third port, and a signal having a level higher than the predetermined level is supplied to the detector immediately after the pump starts driving. Signal supply means, and first pump stop control means for temporarily stopping the pump when the first liquid sensor detects that the liquid has flowed out of the third port. Yes.

  According to the present invention, it is possible to prevent the high concentration component eluted from the column from being discarded through the third port of the multi-way valve when the detector is malfunctioning.

  The liquid chromatograph of the present invention may further include a second liquid sensor that detects whether or not there is a liquid outflow from the second port of the multi-way valve. At this time, the first pump stop control means detects that the first liquid sensor detects the outflow of liquid from the third port or both the first and second sensors are liquid. When it is detected that there is no outflow, the pump is temporarily stopped.

  As a result, it is possible to detect the failure of the detector more reliably, so that the high-concentration components eluted from the column are further discarded through the third port of the multi-way valve.

  In the liquid chromatograph and the control device thereof according to the present invention, the first liquid sensor detects the outflow of liquid from the third port, and the second liquid sensor detects the second port. It is preferable to further include second pump stop control means for temporarily stopping the pump when it is detected that there is a liquid outflow from the pump. Alternatively, in the liquid chromatograph and the control device thereof according to the present invention, the first liquid sensor detects that there is no liquid outflow from the third port, and the second liquid sensor It is preferable to further include second pump stop control means for temporarily stopping the pump when it is detected that there is no liquid outflow from the second port.

  According to this, when the first liquid sensor is out of order, it is possible to prevent the high concentration component eluted from the column from being discarded through the third port of the multi-way valve.

  The liquid chromatograph control program of the present invention is a program that allows a computer to function as the above-described liquid chromatograph control device, and has the same operational effects as the liquid chromatograph control device. This program can be distributed by recording on a removable recording medium such as a CD-ROM (Compact Disc Read Only Memory) disk, flexible disk (FD), MO (Magneto Optical) disk, or a fixed recording medium such as a hard disk. In addition, it can be distributed via a communication network such as the Internet by wired or wireless telecommunication means. Here, the computer is not limited to a general-purpose computer such as a personal computer, but may be a computer incorporated in a device having a specific application.

  Hereinafter, preferred embodiments of the present invention will be described with reference to the drawings.

[First Embodiment]
A schematic configuration of a liquid chromatograph according to a first embodiment of the present invention will be described with reference to FIG. A liquid chromatograph 10 shown in FIG. 1 includes a container 12 in which an eluent 11 is stored, a pump 13, an injector 14 containing a sample, a column 15, a detector 16, and a fraction collector 20. . The pump 13, the detector 16, the fraction collector 20 and the liquid sensor 31 are connected to the control device 19.

  The pump 13 pumps the eluent 11 from the container 12. A sample is stored in the injector 14. When the eluent 11 pumped up from the container 12 by the pump 13 is sent to the injector 14, the sample is sent out from the injector 14 together with the eluent 11.

  Column 15 is packed with silica gel as a stationary phase. By supplying the sample and the eluent 11 to the column 15, the sample is eluted from the column 15 and a liquid chromatograph is performed.

  The detector 16 detects the concentration of the sample eluted from the column 15 based on the absorbance in the flow cell. Then, the detector 16 sends a detection signal to the control device 19.

  The fraction collector 20 includes a test tube support 18 having a large number of test tubes (containers), an arm 17a, and a three-way electromagnetic valve 17b. The three-way solenoid valve 17b has three ports 32a, 32b, and 32c. The port 32 a is an input port connected to the column 15 through the detector 16. The port 32b is a preparative output port to which a nozzle 17c that discharges liquid into the test tube supported by the test tube support 18 is connected. The port 32c is a waste output port to which a drain pipe 33 through which a sample to be discarded passes is connected. In the three-way solenoid valve 17b, based on the drive signal from the control device 19, the port 32a and the port 32b communicate with each other, the port 32a and the port 32c communicate with each other, and the port 32b and the port 32c communicate with each other. Any of the states can be taken. Based on the drive signal from the control device 19, the arm 17a moves the nozzle 17c to a position directly above the test tube of the sorting destination.

  A liquid sensor 31 is attached to the drain pipe 33. The liquid sensor 31 detects whether there is a liquid outflow from the port 32c. If there is a liquid outflow from the port 32 c, the liquid sensor 31 sends a detection signal to the control device 19.

  A liquid sensor 41 is connected to the nozzle 17c to detect whether or not there is a liquid outflow from the port 32b. If there is a liquid outflow from the port 32 b, the liquid sensor 41 sends a detection signal to the control device 19.

  FIG. 2 is an example of the density change curve 51 detected by the detector 16. In this example, the density detection level indicated by the density change curve 51 is lower than the predetermined level 52 before the time a and after the time b, and the density detection indicated by the density change curve 51 during the time w from the time a to the time b. The level is higher than the predetermined level 52. In such a case, in the peak mode, the control device 19 communicates between the port 32a and the port 32c before the time a and after the time b, and during the time w from the time a to the time b, the port 32a And the port 32b are controlled so that the three-way solenoid valve 17b communicates.

  The control device 19 is configured by an information processing device such as a general-purpose personal computer. As shown in FIG. 3, the control device 19 includes a CPU 21, a hard disk 23, and an input / output port 24.

  The input / output port 24 includes, for example, an input unit 25 constituted by a keyboard, a mouse, and the like, a display (display screen) 26 for notifying an operator of various information in the execution process of the liquid chromatograph, and a sorting control described later An arm drive unit 27 that sends a drive signal to the arm 17a based on an instruction from the unit 21a and a valve drive unit 28 that sends a drive signal to the three-way electromagnetic valve 17b based on an instruction from the sorting control unit 21a are connected. Has been. The input unit 25, the display 26, the arm driving unit 27, and the valve driving unit 28 constitute a part of the control device 19. The input / output port 24 is connected to a detector 16 that outputs a concentration signal of the sample eluted from the column 15.

  The arm drive unit 27 generates a drive signal for driving the arm 17a based on the control signal output from the sorting control unit 21a. Based on this drive signal, the arm 17a moves the nozzle 17c to a position directly above the test tube that is the collection destination of the sample eluted from the column 15 among the plurality of test tubes of the test tube support 18. Let

  The valve drive unit 28 generates a drive signal for selecting two communicating ports among the three ports 32a, 32b, 32c of the three-way solenoid valve 17b based on the control signal output by the sorting control unit 21a. .

  As will be described in detail below, the CPU 21 functions as a sorting control unit 21a, a first pump stop control unit 21b, and a second pump stop control unit 21c.

  When the concentration detection level indicated by the detection signal supplied from the detector 16 to the control device 19 is higher than the predetermined level 52, the sorting control unit 21a sends a control signal to the arm driving unit 27 and the valve driving unit 28. The communication between the port 32a and the port 32b of the three-way solenoid valve 17b is made, and the arm 17a is moved right above a new test tube. This initiates the dispensing of the sample into a new test tube. On the other hand, when the concentration detection level indicated by the detection signal supplied from the detector 16 to the control device 19 is lower than the predetermined level 52, the sorting control unit 21a sends a control signal to the arm driving unit 27 and the valve driving unit 28. The three-way solenoid valve 17b communicates between the port 32a and the port 32c.

  When the first pump stop control unit 21b detects that the liquid sensor 31 has an outflow of liquid from the port 32c even though the concentration detection level indicated by the concentration change curve 51 is higher than the predetermined level 52, or When both the liquid sensors 31 and 42 detect that there is no liquid outflow, a pump stop signal is sent to the pump 13 to temporarily stop the pump 13. In such a case, if it is known in advance that the detector 16 has not failed, the probability that the three-way solenoid valve 17b has failed is high. In addition, when the concentration detection level is lower than the predetermined level, the liquid sensor 31 does not detect that there is a liquid outflow from the port 32c, or both the liquid sensors 31 and 42 have a liquid outflow. Even when this is detected, the first pump stop control unit 21b sends a pump stop signal to the pump 13 to temporarily stop the pump 13. Also in this case, if it is known in advance that the detector 16 has not failed, there is a high probability that the three-way solenoid valve 17b has failed.

  The second pump stop control unit 21c detects that the liquid sensor 41 has an outflow of liquid from the port 32b even though the liquid sensor 31 has detected the outflow of liquid from the port 32c. If so, a pump stop signal is sent to the pump 13 to stop the pump 13 temporarily. This is because if it is known in advance that the liquid sensor 41 has not failed, the liquid sensor 31 has failed. In addition, even when the liquid sensor 41 detects that there is no liquid outflow from the port 32b even though the liquid sensor 31 has detected that there is no liquid outflow from the port 32c, the second The pump stop control unit 21c sends a pump stop signal to the pump 13 to stop the pump 13 temporarily. Also in this case, if it is known in advance that the liquid sensor 41 has not failed, the liquid sensor 31 has failed.

  The hard disk 23 stores various software including a liquid chromatograph control program according to the present embodiment. The above-described units 21a, 21b, and 21c are constructed by combining these hardware and software. This program can be installed in any computer by recording it on a removable recording medium such as a CD-ROM, FD, or MO.

  Next, the procedure for performing the liquid chromatograph in the peak mode will be described based on the flowchart shown in FIG. It is assumed that the detector 16 has not failed and the liquid sensor 41 has not failed.

  In step S1 of FIG. 4, the pump 13 is driven to start a liquid chromatograph.

  Next, in step S2, the liquid sensor 31 and the liquid sensor 41 both detect the liquid flow, or the liquid sensor 31 and the liquid sensor 41 both do not detect the liquid flow, or neither ( That is, it is determined which one of the liquid sensors is detecting the flow of the liquid). If only one of the liquid sensor 31 and the liquid sensor 41 detects the liquid, the liquid sensor 31 is functioning normally, and the process proceeds to step S6. When the liquid sensor 31 and the liquid sensor 41 both detect the flow of the liquid, or when both the sensors 31 and 42 do not detect the flow of the liquid (step S2: YES), the liquid sensor 41 has not failed. Therefore, the liquid sensor 31 is out of order. Then, it transfers to step S3 and the 2nd pump stop control part 21c sends out a pump stop signal to the pump 13, and stops the pump 13 temporarily.

  After the pump is temporarily stopped in step S3, the process proceeds to step S4, and the operator replaces, repairs, or checks the liquid sensor 31. If the malfunction of the liquid sensor 31 is eliminated, the process proceeds to step S5 and the driving of the pump 13 is resumed. The restart of the pump 13 is triggered by a manual operation by an operator such as pressing a switch.

  Next, the process proceeds to step S6, and it is determined whether the detection level of the detector 16 is equal to or higher than a predetermined level. If the detection level of the detector 16 is equal to or higher than the predetermined level (step S6: YES), the process proceeds to step S7. If the detection level of the detector 16 is less than the predetermined level (step S6: NO), the determination in step S6 is repeated until the detector 16 detects a detection level equal to or higher than the predetermined level.

  Next, in step S7, the liquid sensor 31 has detected that there is an outflow of liquid from the port 32c, or neither the liquid sensor 31 nor the liquid sensor 41 has detected the liquid flow, or neither ( That is, it is determined whether only the liquid sensor 41 is detecting the flow of the liquid). If only the latter, that is, only the liquid sensor 41 detects the flow of the liquid (step S7: NO), the sample to be sorted is flowing toward the nozzle 17c, and the process proceeds to step S8. In the former case, that is, when the liquid sensor 31 detects that there is a liquid outflow from the port 32c, or when both the liquid sensor 31 and the liquid sensor 41 have not detected the liquid flow (step S7: YES), the sample to be collected However, due to the malfunction of the three-way solenoid valve 17b, the sample is accidentally discharged only to the drain pipe 33, the sample is discharged to both the nozzle 17c and the drain pipe 33, or the sample is three-way solenoid valve 17b is not discharged, the process proceeds to step S11, and the first pump stop control unit 21b sends a pump stop signal to the pump 13 to temporarily stop the pump 13.

  After temporarily stopping the pump 13 in step S11, the process proceeds to step S12, and the operator replaces or repairs the three-way solenoid valve 17b. If the malfunction of the three-way solenoid valve 17b is removed, the process proceeds to step S13, and the drive of the pump 13 is resumed. The restart of the pump 13 is triggered by a manual operation by an operator such as pressing a switch. Thereafter, the process proceeds to step S8.

  Next, in step S8, the sorting control unit 21a controls the three-way solenoid valve 17b and the arm 17a, so that the sorting of the sample to be sorted is started with a new test tube. In step S9, it is repeatedly determined whether the detection level of the detector 16 is less than a predetermined level. If the detection level of the detector 16 is equal to or higher than the predetermined level (step S9: NO), sample collection is continued. And if the detection level of the detector 16 becomes less than a predetermined level (step S9: YES), it will transfer to step S10 and will complete | finish sorting.

  As described above, according to the present embodiment, when the liquid chromatography is performed in the peak mode, it is possible to easily find the malfunction of the three-way solenoid valve 17b and the liquid sensor 31, and the necessary components can be found even if there is a malfunction. It is possible to prevent wasteful discharge.

[Second Embodiment]
A second embodiment of the present invention will be described with reference to FIGS. In addition, the same code | symbol is attached | subjected to the member same as 1st Embodiment, and the description is abbreviate | omitted. The configuration of the second embodiment is different from the first embodiment in that the signal supply unit 21d is provided in the CPU 21 of the control device 19 in the configuration diagram of the control device 19 shown in FIG. The control device 19 is provided with a memory 22 having a signal storage unit 22a.

  The signal supply unit 21d automatically supplies a level signal (dummy signal) of a predetermined level or higher to the detector 16 automatically immediately after the driving of the pump 13 is started. If the detector 16 detects this dummy signal, the detection signal is input to the sorting control unit 21a via the input / output port 24, and sorting is performed. If the detector 16 does not detect this signal, the control by the sorting control unit 21a is not performed.

  The signal storage unit 22a stores the signal level of the dummy signal and supplies the level information of the dummy signal to the signal supply unit 21d. The signal supply unit 21 d generates a dummy signal that is a pulse signal having a signal level supplied from the signal storage unit 22 a in a signal generation circuit (not shown), and supplies the dummy signal to the detector 16.

  The procedure for performing liquid chromatography in the peak mode will be described based on the flowchart shown in FIG. It is assumed that the three-way solenoid valve 17b has not failed and the liquid sensor 41 has not failed.

  Steps S1 to S5 are the same as those in the first embodiment, and a description thereof will be omitted. After step S5, the process proceeds to step S21, where the signal supply unit 21d generates a dummy signal and supplies it to the detector 16.

  Next, the process proceeds to step S22, in which the liquid sensor 31 detects that there is a liquid outflow from the port 32c, or both the liquid sensor 31 and the liquid sensor 41 do not detect the liquid flow, or any of them. However, it is determined whether it is (that is, only the liquid sensor 41 is detecting the flow of the liquid). In the latter case, that is, when only the liquid sensor 41 detects the flow of the liquid (step S22: NO), since the detector 16 has correctly detected the dummy signal, the sorting control by the sorting control unit 21a is performed, and the sample is ejected from the nozzle. Since it flows to 17c and the sample can be sorted into the test tube, the process proceeds to step S6. In the former case, that is, when the liquid sensor 31 detects that the liquid is flowing out from the port 32c, or when both the liquid sensor 31 and the liquid sensor 41 have not detected the liquid flow (step S22: YES), the detector 16 The sorting control by the sorting control unit 21a does not work due to the malfunction, the sample is accidentally discharged toward the drain pipe 33, the sample is discharged to both the nozzle 17c and the drain pipe 33, Alternatively, the sample is either not discharged from the three-way solenoid valve 17b, and the process proceeds to step S23, where the first pump stop control unit 21b sends a pump stop signal to the pump 13, whereby the pump 13 Is paused.

  After the pump 13 is temporarily stopped in step S23, the process proceeds to step S24, where the operator replaces or repairs the detector 16. After the defect of the detector 16 is removed, the process proceeds to step S25, the driving of the pump 13 is resumed, and the process proceeds to step S6. The restart of the pump 13 is triggered by a manual operation by an operator such as pressing a switch. Since step S6 and steps S8 to S10 are the same as those in the first embodiment, the description thereof is omitted.

  As described above, according to the present embodiment, when the liquid chromatography is performed in the peak mode, the failure of the detector 16 and the liquid sensor 31 can be easily found, and necessary components are wasted even if there is a failure. Can be prevented from being discharged.

  Although the present invention has been described based on the preferred embodiments, the present invention can be modified without departing from the spirit of the present invention. That is, the liquid sensor 41 may not be attached to the nozzle 17c. In this case, it is not necessary to provide the second pump stop control unit 21c in the CPU 21 of the control device 19. A four-way valve or the like may be used as the multi-way valve. In step S7 and step S22, it may be determined only whether the liquid sensor 31 has detected a flow, and the pump may be temporarily stopped when the liquid sensor 31 has detected a flow.

  In addition, the liquid chromatograph control program may be written in the ROM of the storage unit in advance for reading only, or the one recorded on a recording medium such as a CD is read out and written to the storage unit when necessary. Alternatively, it may be transmitted via a telecommunication line such as the Internet and written in the storage unit.

1 is a schematic configuration diagram of a liquid chromatograph according to a first embodiment of the present invention. It is a graph showing the time-dependent change of the density | concentration level of a sample. It is a block diagram of the control apparatus of the liquid chromatograph which concerns on the 1st Embodiment of this invention. It is a flowchart for demonstrating operation | movement of the liquid chromatograph which concerns on the 1st Embodiment of this invention. It is a block diagram of the control apparatus of the liquid chromatograph which concerns on the 2nd Embodiment of this invention. It is a flowchart for demonstrating operation | movement of the liquid chromatograph which concerns on the 2nd Embodiment of this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 10 Liquid chromatograph 11 Eluent 12 Container 13 Pump 14 Injector 15 Column 16 Detector 17a Arm 17b Three-way solenoid valve (multi-way valve)
17c Nozzle 18 Test tube support 19 Control device 20 Fraction collector 21 CPU
21a Sorting control unit 21b First pump stop control unit (first pump stop control means)
21c 2nd pump stop control part (2nd pump stop control means)
21d Signal supply unit (signal supply means)
22 memory 22a signal storage unit 23 hard disk 24 input / output port 25 input unit 26 display screen display 27 arm drive unit 28 valve drive unit 31 liquid sensors 32a, 32b, 32c port 33 drain pipe 41 liquid sensor 52 predetermined level

Claims (18)

A column packed with a stationary phase;
A pump for supplying an eluent to the column;
A detector for detecting the concentration of a component contained in the sample eluted from the column;
A fraction collector for separating a sample that has been eluted from the column and then supplied to the first port of the multi-way valve into a plurality of containers via the second port of the multi-way valve;
A first liquid sensor that detects whether there is liquid outflow from the third port of the multi-way valve;
When the concentration detection level by the detector is higher than a predetermined level, the first port and the second port of the multi-way valve communicate with each other, and the concentration detection level by the detector is lower than the predetermined level. Valve control means for controlling the multi-way valve so that the first port and the third port of the multi-way valve communicate with each other when the
When the concentration detection level by the detector is higher than a predetermined level and the first liquid sensor detects that there is a liquid outflow from the third port, a first stop is made to temporarily stop the pump. A liquid chromatograph comprising pump stop control means. A second liquid sensor for detecting whether or not there is a liquid outflow from the second port of the multi-way valve;
When the first pump stop control means detects that the concentration detection level by the detector is higher than a predetermined level and the first liquid sensor detects the outflow of liquid from the third port. The liquid chromatograph according to claim 1, wherein when both the first and second sensors detect that there is no liquid outflow, the pump is temporarily stopped. A column packed with a stationary phase;
A pump for supplying an eluent to the column;
A detector for detecting the concentration of a component contained in the sample eluted from the column;
A fraction collector for separating a sample that has been eluted from the column and then supplied to the first port of the multi-way valve into a plurality of containers via the second port of the multi-way valve;
A first liquid sensor that detects whether there is liquid outflow from the third port of the multi-way valve;
When the concentration detection level by the detector is higher than a predetermined level, the first port and the second port of the multi-way valve communicate with each other, and the concentration detection level by the detector is lower than the predetermined level. Valve control means for controlling the multi-way valve so that the first port and the third port of the multi-way valve communicate with each other when the
Signal supply means for supplying a signal having a level higher than the predetermined level to the detector immediately after the start of driving of the pump;
And a first pump stop control means for temporarily stopping the pump when the first liquid sensor detects the outflow of liquid from the third port. Liquid chromatograph. A second liquid sensor for detecting whether or not there is a liquid outflow from the second port of the multi-way valve;
The first pump stop control means detects that the first liquid sensor detects that there is a liquid outflow from the third port or that both the first and second sensors have liquid outflow. The liquid chromatograph according to claim 3, wherein the pump is temporarily stopped when it is detected that there is not.   When the first liquid sensor detects that there is a liquid outflow from the third port, and the second liquid sensor detects that there is a liquid outflow from the second port The liquid chromatograph according to claim 2, further comprising second pump stop control means for temporarily stopping the pump.   When the first liquid sensor detects that no liquid flows out from the third port, and the second liquid sensor detects that no liquid flows out from the second port The liquid chromatograph according to claim 2, further comprising second pump stop control means for temporarily stopping the pump. A column filled with a stationary phase, a pump for supplying an eluent to the column, a detector for detecting the concentration of a component contained in the sample eluted from the column, and a multi-way valve after elution from the column. A fraction collector that separates the sample supplied to the first port into a plurality of containers via the second port of the multi-way valve, and whether or not there is a liquid outflow from the third port of the multi-way valve. A liquid chromatograph control device comprising a first liquid sensor to detect,
When the concentration detection level by the detector is higher than a predetermined level, the first port and the second port of the multi-way valve communicate with each other, and the concentration detection level by the detector is lower than the predetermined level. Valve control means for controlling the multi-way valve so that the first port and the third port of the multi-way valve communicate with each other when the
When the concentration detection level by the detector is higher than a predetermined level and the first liquid sensor detects that there is a liquid outflow from the third port, a first stop is made to temporarily stop the pump. A liquid chromatograph control device comprising pump stop control means. The liquid chromatograph further comprises a second liquid sensor for detecting whether or not there is an outflow of liquid from the second port of the multi-way valve;
When the first pump stop control means detects that the concentration detection level by the detector is higher than a predetermined level and the first liquid sensor detects the outflow of liquid from the third port. 8. The liquid chromatograph control device according to claim 7, wherein when both the first and second sensors detect that no liquid flows out, the pump is temporarily stopped. A column filled with a stationary phase, a pump for supplying an eluent to the column, a detector for detecting the concentration of a component contained in the sample eluted from the column, and a multi-way valve after elution from the column. A fraction collector that separates the sample supplied to the first port into a plurality of containers via the second port of the multi-way valve, and whether or not there is a liquid outflow from the third port of the multi-way valve. A liquid chromatograph control device comprising a first liquid sensor to detect,
When the concentration detection level by the detector is higher than a predetermined level, the first port and the second port of the multi-way valve communicate with each other, and the concentration detection level by the detector is lower than the predetermined level. Valve control means for controlling the multi-way valve so that the first port and the third port of the multi-way valve communicate with each other when the
Signal supply means for supplying a signal having a level higher than the predetermined level to the detector immediately after the start of driving of the pump;
And a first pump stop control means for temporarily stopping the pump when the first liquid sensor detects the outflow of liquid from the third port. Control device for liquid chromatograph. The liquid chromatograph further comprises a second liquid sensor for detecting whether or not there is an outflow of liquid from the second port of the multi-way valve;
The first pump stop control means detects that the first liquid sensor detects that there is a liquid outflow from the third port or that both the first and second sensors have liquid outflow. The liquid chromatograph control device according to claim 9, wherein when the absence is detected, the pump is temporarily stopped.   When the first liquid sensor detects that there is a liquid outflow from the third port, and the second liquid sensor detects that there is a liquid outflow from the second port 11. The liquid chromatograph control device according to claim 8, further comprising second pump stop control means for temporarily stopping the pump.   When the first liquid sensor detects that no liquid flows out from the third port, and the second liquid sensor detects that no liquid flows out from the second port 11. The liquid chromatograph control device according to claim 8, further comprising second pump stop control means for temporarily stopping the pump. A column filled with a stationary phase, a pump for supplying an eluent to the column, a detector for detecting the concentration of a component contained in the sample eluted from the column, and a multi-way valve after elution from the column. A fraction collector that separates the sample supplied to the first port into a plurality of containers via the second port of the multi-way valve, and whether or not there is a liquid outflow from the third port of the multi-way valve. A chromatograph control program for causing a computer to control a liquid chromatograph including a first liquid sensor to be detected,
When the concentration detection level by the detector is higher than a predetermined level, the first port and the second port of the multi-way valve communicate with each other, and the concentration detection level by the detector is lower than the predetermined level. A valve control means for controlling the multi-way valve so that the first port and the third port of the multi-way valve communicate with each other when
When the concentration detection level by the detector is higher than a predetermined level and the first liquid sensor detects that there is a liquid outflow from the third port, a first stop is made to temporarily stop the pump. A liquid chromatograph control program for causing a computer to function as a pump stop control means. A chromatograph control program for causing a computer to control the liquid chromatograph further comprising a second liquid sensor for detecting whether or not there is a liquid outflow from the second port of the multi-way valve,
When the first pump stop control means detects that the concentration detection level by the detector is higher than a predetermined level and the first liquid sensor detects the outflow of liquid from the third port. 14. The liquid chromatograph control program according to claim 13, wherein when both the first and second sensors detect that no liquid flows out, the pump is temporarily stopped. A column filled with a stationary phase, a pump for supplying an eluent to the column, a detector for detecting the concentration of a component contained in the sample eluted from the column, and a multi-way valve after elution from the column. A fraction collector that separates the sample supplied to the first port into a plurality of containers via the second port of the multi-way valve, and whether or not there is a liquid outflow from the third port of the multi-way valve. A chromatograph control program for causing a computer to control a liquid chromatograph including a first liquid sensor to be detected,
When the concentration detection level by the detector is higher than a predetermined level, the first port and the second port of the multi-way valve communicate with each other, and the concentration detection level by the detector is lower than the predetermined level. Valve control means for controlling the multi-way valve so that the first port and the third port of the multi-way valve communicate with each other when
Signal supply means for supplying a signal having a level higher than the predetermined level to the detector immediately after the start of driving of the pump; and
A liquid chromatograph control program for causing a computer to function as first pump stop control means for temporarily stopping the pump when the first liquid sensor detects that there is a liquid outflow from the third port . A chromatograph control program for causing a computer to control the liquid chromatograph further comprising a second liquid sensor for detecting whether or not there is a liquid outflow from the second port of the multi-way valve,
The first pump stop control means detects that the first liquid sensor detects that there is a liquid outflow from the third port or that both the first and second sensors have liquid outflow. The liquid chromatograph control program according to claim 15, wherein when the absence is detected, the pump is temporarily stopped.   When the first liquid sensor detects that there is a liquid outflow from the third port, and the second liquid sensor detects that there is a liquid outflow from the second port The liquid chromatograph control program according to claim 14 or 16, further causing a computer to function as second pump stop control means for temporarily stopping the pump.   When the first liquid sensor detects that no liquid flows out from the third port, and the second liquid sensor detects that no liquid flows out from the second port The liquid chromatograph control program according to claim 14 or 16, further causing a computer to function as second pump stop control means for temporarily stopping the pump.

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