JP4159898B2 - Warm sterilization method of transplanted bone - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention can be applied to bone grafts having various different properties, shapes, and sizes. Warm sterilization method About.
[0002]
[Prior art]
Bone transplantation is often required during surgery, and examples of indications include filling and repair after focal curettage such as bone tumors, bridging of bone defects that occurred after malignant tumor resection or trauma, and bone attachment Acquisition of bones, repair of bone defects by artificial joint replacement, and the like. In the case of a large bone defect, it cannot be filled with autologous bone alone, so another filling material is required. Artificial bone replacement materials have recently been developed as a replacement material, but homogenous bones are more useful because they are replaced by autologous bones through the processes of bone induction and bone conduction. However, fresh allogeneic bone is different from autologous bone in that it has antigenicity. Therefore, antigenicity is usually reduced by chemical treatment or freezing.
[0003]
Furthermore, in order to minimize the risk of infection, particularly the risk of viral infection during bone transplantation, the transplanted bone must undergo appropriate virus inactivation and sterilization prior to surgery. When the entire transplanted bone or a part of the bone is frozen after being collected and stored in the bone bank, the sterilization treatment is performed before the storage. The bactericidal treatment is performed for the purpose of preventing infectious diseases, particularly viral infection, but it must not destroy the biomechanical properties of bone and maintain the protein structure. These two requirements can be met by heating the entire bone to a temperature of about 80 ° C. over a period of time.
[0004]
This sterilization condition of keeping the temperature of the transplanted bone at a minimum of 80 ° C. for a certain time, for example, 10 minutes, has already been widely adopted for the femoral head heating treatment. For example, a warmer sterilizer “Lobator SD-2 (trade name)” for a femoral head collected from a donor who has undergone surgery (biological donor) is disclosed in US Pat. No. 5,591,398 (EP 05848484 A1). This condition is realized by the processing method described in (1). However, this device is a processing device exclusively for the femoral head and cannot be used for other bones.
[0005]
In the sterilization method of Robotator SD-2, the femoral head is placed in a plastic cylindrical special container and heated. The dedicated container is adapted to fit in the cylindrical heating cavity of the device. The container is filled with Lactated Ringer's solution to a predetermined level and sealed with a dedicated lid. The lid is equipped with a self-sealing stopper that can be inserted. After the heating process is completed, the lactated Ringer's solution is removed from the dedicated container using another container equipped with an injection needle, and necessary ventilation is performed. In the method of the prior patent invention, the lactated Ringer's solution functions as a medium for convective heat transfer from the container wall to the femoral head. The dedicated container is equipped with a magnetic stirrer to eliminate the effects of different thermal resistance on the inside of the container wall, Lactated Ringer's solution itself, the femoral head surface, etc., and prevent local temperature peaks and local overheating. ing. This stirrer is provided below the femoral head in the container. Therefore, the femoral head is placed on the wire screen so that a constant distance is maintained between the inner bottom of the container and the femoral head, and the stirring bar rotates below the wire screen.
[0006]
Lactated Ringer's solution is injected and the sealed plastic container is placed in the electrical heating cavity of the device where it is heated. Due to the structure of the device, it is impossible to measure the temperature of any part in the container such as the temperature of each part of the transplanted bone or the temperature of the lactated Ringer's solution during the heating process. Therefore, all of the temperature control of the heating cavity such as the heating time or the heating power must be set empirically.
[0007]
The treatment described in USP 5,591,398 is effective for relatively small grafts of the same type such as the femoral head for economic and technical reasons. It is not suitable for processing by this method because it does not enter the cylindrical dedicated container or cannot be completely immersed in Ringer's solution. Therefore, a completely new device needs to be developed to sterilize other types of bone grafts.
[0008]
This method has its limitations other than economic reasons. To enlarge the cylindrical container so that a sterilization container for the proximal part of the tibia, for example about 20 cm in length, can be placed on the wire screen, it is dedicated to contain the bone graft and heat transfer liquid All objects to be heated, such as auxiliary equipment and agitators, as well as containers, must be enlarged. Therefore, the heat capacity of the object to be heated increases, and it takes a considerably long time to raise the temperature. Even at the temperature rising stage alone, the transplanted bone is exposed to a high temperature for a long time, and effective sterilization of at least 10 minutes is possible. Before the time to be done, it can damage the biological properties of the transplanted bone. Thus, the ratio between the effective time for sterilization and the actual warming time deteriorates as the container is made larger.
[0009]
As another treatment method, there is a method described in DE 40 37 806 A1. This processing method has relatively no problem in the technology, but has a drawback that the apparatus is complicated and expensive. The grafted bone is stored in a thermostatic bath filled with a heat transfer liquid (eg, made of sterilized water). Warming requires two separate and different warming systems. The thermostat itself is placed in a second liquid tank filled with the second heat transfer liquid. This second heat transfer liquid, usually made of water, is circulated by a pump through an outer loop containing a buffer tank, a heat exchanger and an electric warmer. Furthermore, microwave energy is irradiated through the lid of the thermostatic bath, and the transplanted bone directly absorbs this energy and is also directly heated by water. Therefore, the temperature chamber lid must be permeable to high frequency electromagnetic energy irradiation.
[0010]
In the case where the second heat transfer liquid tries to heat the transplanted bone only by circulating through the outer loop, the heat capacity of the object to be heated is excessive, and it takes a lot of time for the heating. Therefore, in order to shorten the heating time within an allowable range, a second heating system using microwave energy is indispensable.
[0011]
These two warming systems are controlled by two separate temperature sensors. One of the temperature sensors must be placed in the center of the bone graft. Therefore, in order to install the temperature sensor at the center of the bone graft, a hole must be provided in the bone graft by drilling. According to this processing method, it is possible to suitably sterilize bones of various types, shapes and sizes by providing such two heating systems. However, this method requires two types of heating devices, two liquid water tanks, an external loop pipe, a pump, a valve, a buffer tank, and the like, and has a drawback of being complicated and expensive.
[0012]
Therefore, as disclosed in Japanese Patent Application Laid-Open No. 2002-017826, the present applicant heats the transplanted bone with a predetermined temperature only by microwave irradiation for a predetermined time, and then heats and sterilizes the transplanted bone. Was proposed earlier. According to this method, optimum warming sterilization according to the type, shape and size of the transplanted bone can be easily achieved in a short time. However, in order to optimize the conditions of heat sterilization, it is necessary to precisely monitor the temperature of the transplanted bone, and a special and expensive sensor is required for temperature measurement, or a large amount of temperature measurement is required. There was a problem to be solved, such as the need for labor.
[0013]
Therefore, as a result of various experiments and researches by the applicant, it is possible to easily measure the temperature of the atmosphere in the container of the transplanted bone, and it is possible to perform optimum heat sterilization only by monitoring this temperature. I found out.
[0014]
[Problems to be solved by the invention]
In view of the problems of the prior art and the inventor's knowledge, the main object of the present invention is to make it possible to optimally and easily sterilize transplanted bones of various types, shapes and sizes. Warm sterilization method Is to provide.
[0015]
The second object of the present invention is to economically sterilize the transplanted bone without requiring a complicated or expensive auxiliary device. Warm sterilization method Is to provide.
[0016]
The third object of the present invention is to , Easy to operate, suitable for direct use in the operating room Warm sterilization method Is to provide.
[0017]
[Means for Solving the Problems]
Such a problem is, according to the present invention, a method for warming and sterilizing a transplanted bone, comprising: a microwave irradiation source and a microwave irradiation device including a microwave cavity to be irradiated with a microwave by the microwave irradiation source A step of preparing an apparatus, a step of preparing a sealed aseptic heating container formed of a material that at least partially transmits microwaves, and a temperature sensor for measuring the temperature of the atmosphere in the heating container. A step of placing in the warming vessel, a step of placing the grafted bone to be sterilized in the warming vessel, the warming vessel in which the grafted bone is placed in the microwave cavity, and the grafted bone On the other hand, in the process of irradiating microwaves from the microwave irradiation source, and in the irradiation process, the amount of microwave irradiation from the microwave irradiation source is controlled according to the output of the temperature sensor. It is achieved by providing a heating sterilization method of bone graft, characterized by having the steps of.
[0018]
Measuring the temperature of the atmosphere in the heating container can be easily performed by using a thermocouple or a semiconductor temperature sensor, and according to the knowledge of the inventor, various results are obtained from the measurement result of such a temperature sensor. It is possible to optimally and easily sterilize transplanted bones of different types, shapes and sizes.
[0019]
The heating container is handled after being sterilized by being stored frozen, transported, etc., but the heating container should receive the temperature sensor so as to substantially maintain its airtightness. If it has an opening, not only can the sterilization process be performed easily, but the internal aseptic condition can be easily maintained even when handled in various ways. The self-sealing opening can also be used for taking a sample of a transplanted bone without breaking the sterility by using an injection needle or the like. Alternatively, if the heating container incorporates the temperature sensor and has an opening for pulling out the lead of the temperature sensor substantially airtightly, the temperature sensor is required for each container. Although it is lost due to disposal of the container, sterilization treatment and other handling can be easily performed, and management advantages can be obtained.
[0020]
Depending on the irradiation intensity of the microwave and the characteristics of the temperature sensor, the output of the temperature sensor may be subject to interference due to the current induced by the microwave. By detecting the output of the temperature sensor only during the rest period, or by surrounding the temperature sensor with a shield made of a wire mesh or the like, interference due to the current induced by the microwave can be avoided.
[0021]
Such a method is a sealed aseptic warming container for accommodating the transplanted bone when the transplanted bone is sterilized by heating with a microwave irradiation device, and at least a part thereof is formed of a material that transmits microwaves. It is preferably carried out by using a sealed aseptic heating container characterized by having a sealed container, a temperature sensor disposed in the sealed container, and a humidifying medium disposed in the sealed container. be able to.
[0022]
When the transplanted bone is subjected to heat sterilization treatment, keeping the transplanted bone moist is important as discussed in Japanese Patent Application Laid-Open No. 2002-017826, and as disclosed in the same publication, A screen or a screen for avoiding the submergence of the water storage part and the transplanted bone can be used in the heating container, but for convenience, a sponge or a sponge for holding the humidifying medium in the heating container It is preferable to have a porous member made of foamed resin or the like.
[0023]
DETAILED DESCRIPTION OF THE INVENTION
FIG. 1 shows a warming sterilizer according to the present invention. The warming sterilizer 1 is provided with a housing having a glass door that opens from the front, like a normal home microwave oven. A bone graft (not shown) is accommodated in a sealed container 3, and the sealed container 3 is placed on the installation table 2. In the case of the present embodiment, the installation base 2 is fixed, and in order to uniformly irradiate the microwave toward the irradiated object from a microwave irradiation source (not shown), the movable reflector 5 is appropriately placed in the housing. Has been placed. The installation base 2 is made of a material that generally does not absorb microwaves. In addition to this, or instead of this, microwave irradiation can be made uniform by using the installation table as a turntable and rotating it appropriately.
[0024]
A circular recess is provided at the center of the installation base 2, and a thermocouple-type temperature sensor 4, which has an injection needle shape, protrudes from the center of the bottom. A thermocouple is fixed to the tip of the temperature sensor 4 and enters the sealed container 3 as described below.
[0025]
The temperature in the airtight container 3 obtained by the temperature sensor 4 is monitored during the sterilization treatment, and based on this, the control unit 10 controls not only the microwave irradiation amount but also the operation of the reflection device 5, for the shortest time. In addition, a predetermined temperature is achieved throughout the transplanted bone, and this temperature is maintained for a predetermined time. A display 7 and keyboards 8 and 9 for operating the control unit 10 are provided on the front surface of the housing.
[0026]
The container 3 shown in FIG. 2 has a configuration suitable for microwave irradiation while humidifying the surface of the transplanted bone. The container 3 has a bottomed cylindrical container body 11 made of a hard resin material, and the bottom of the container 3 is fitted into the central recess 2 a of the installation base 2, whereby the container body 11 is positioned. A cylindrical collar 13 protruding inward in the axial direction is provided at the center of the inner surface of the bottom of the container main body, and the tip thereof forms three protrusions 14 separated from each other by a gap in the circumferential direction. .
[0027]
A lid 15 is screwed into an upper end of the container body 11, that is, an open end via a seal 12 made of an O-ring. The lid 15 has a cup shape, and an inner screw provided on the inner periphery of the axial flange thereof is screwed with an outer screw provided on the outer periphery of the container body open end. On the outer periphery of the axial flange, an uneven portion 17 is provided so as not to slip when it is gripped and rotated. On the inner surface of the lid 15, a collar 16 that protrudes into the container body 11 is provided. Foamed resin pieces 18 and 19 for impregnating physiological saline or the like for wetting the transplanted bone are provided in the recess surrounded by the collar 16 and in the annular recess on the outer periphery of the cylindrical collar 13 at the bottom of the container body. It is accepted.
[0028]
The center of the cylindrical collar 13 at the bottom of the container body is generally hollow and defines a hole communicating with the inside and outside of the container, but is closed by a self-sealing rubber plug 20. The rubber plug 20 may be provided on a bag for a drug for injection, and may be made of one that is widely used to allow the injection needle to enter and allow the drug to be inhaled, or an equivalent thereof. Further, an annular groove 11a is provided around the outer end of the cylindrical collar 13, and a rubber cap 6 is attached to the outer end of the cylindrical collar 13 as shown in FIG. Contamination from the rubber plug 20 can be prevented.
[0029]
A threaded boss 2b fixed to the metal wall plate 1a of the microwave cavity of the warming sterilizer 1 protrudes from the center of the bottom surface of the central recess 2a of the installation table 2, and the diameter expansion base of the temperature sensor 4 is It is fixed to this threaded boss 2b by a cap nut 2c. Corresponding contact terminals are provided at the bases of the threaded boss 2b and the temperature sensor 4, respectively, so that the output of the temperature sensor can be sent to the control unit 10 via the lead 4a. In the illustrated embodiment, the temperature sensor 4 is provided at the bottom of the warming sterilizer 1 and the container 3 is vertically placed so as to penetrate the rubber plug 20 provided at the bottom. Alternatively, the temperature sensor 4 can be provided on the side wall and the upper wall of the warming sterilizer 1, and the temperature sensor 4 is not limited to the bottom of the container 3 but also enters the container 3 from the side or top thereof. Can be.
[0030]
According to the above-described embodiment, first, the foamed resin pieces 18 and 19 are appropriately impregnated with sterilized water or physiological saline, which is placed in the container 3, and then the transplanted bone is placed in the container 3. Further, when the container 3 is pushed into the central recess 2a of the installation base 2 from the bottom side, the container 3 is positioned at the center by the central recess 2a, and as it is pushed, the temperature sensor 4 penetrates the rubber plug 20 and the container 3 Rush into. In this state, the three protrusions 14 serve to protect the temperature sensor 4. In this state, the door of the warming sterilizer 1 is closed, and microwaves are irradiated from the microwave irradiation source into the container 3. Thereby, since the transplanted bone is heated not only from its surface but also from the inside, the time required for raising the temperature to a certain temperature such as 80 ° C. can be shortened. While controlling the temperature based on the output from the temperature sensor 4 and heating the bone graft for a predetermined time, the microwave irradiation source is stopped. If the temperature of the transplanted bone exceeds the protein denaturation temperature, the transplanted bone will not be suitable for transplantation, and if the temperature is too low or the heating time is too short, sterilization will be insufficient. A database of heating conditions according to conditions such as the type, size, and shape of the transplanted bone, and the efficiency of the transplanted bone by controlling the irradiation energy of the microwave irradiation source based on the output from the temperature sensor 4 Can be sterilized efficiently and reliably.
[0031]
Depending on the irradiation intensity of the microwave and the characteristics of the temperature sensor 4, the output of the temperature sensor 4 may be interfered by a current induced by the microwave. In such a case, it is possible to avoid interference due to the current induced by the microwave by irradiating the microwave in a pulse shape and detecting the output of the temperature sensor only during the rest period of the microwave pulse. .
[0032]
4 and 5 show a second embodiment of the present invention. Parts corresponding to those of the above-mentioned embodiment are denoted by the same reference numerals, and detailed description thereof is omitted. In the present embodiment, the sealed container 3 ′ has a configuration suitable for horizontal placement. Depending on the type of bone to be transplanted, it may be necessary to keep it stable in the container, and it may be desirable to place the container on its side. This container has a configuration substantially similar to that shown in FIGS. 2 and 3, but in order to be placed horizontally, a flat lower mounting surface 11c is defined on the side wall of the container to be lower. A pair of leg pieces 11b to be formed are integrally formed. A perforated plate 29 defining a flat upper support surface 29a for supporting the graft bone B is fixed to the inner peripheral portion of the container to be the lower side by an adhesive or other means.
[0033]
The leg piece 11b makes it possible to stably place the sealed container 3 'on the metal wall plate 1a of the microwave cavity of the warming sterilizer 1, but freezes the sealed container 11' after warming and sterilizing it. It is also useful for stable handling of the container when it is stored, stored, or transported. Further, a guide structure (not shown) that cooperates with these leg pieces 11b is provided on the metal wall plate 1a, and the temperature when the container 3 'is placed in the microwave cavity of the warming sterilizer 1 is changed. It is also possible to easily and surely penetrate the rubber plug 20 provided with the sensor 4 in the container.
[0034]
FIG. 6 shows a third embodiment of the present invention, and parts corresponding to those of the above-mentioned embodiment are denoted by the same reference numerals and detailed description thereof is omitted. In the present embodiment, the structure of the bottom portion of the sealed container 3 ″ is slightly different from the above-described embodiment, and the container bottom portion 22 has a bottomed cylindrical shape slightly smaller in diameter than the container body 11 as shown in the right half of FIG. And is connected to the container body 11 only through the annular thin portion 21. The left half of Fig. 6 has an inner screw 25 that is screwed into an outer screw 24 provided on the outer periphery of the container bottom portion 22. 6 shows a state where the screw ring 23 is mounted, in the embodiment shown in the right half of Fig. 6, in order to hold the container body 11 stably, it is provided on the container body 11 side for mounting the screw ring 23. The outer screw 24 is screwed into the corresponding inner screw provided on the side of the installation base 2. In this way, it is possible to resist gravity and to freely install the container main body 11 in a desired direction. Can be selected.
[0035]
In the embodiment shown in FIGS. 2 and 3, the installation table 2 is composed of three members arranged at intervals of 120 degrees. In this embodiment, the installation table 2 is arranged at intervals of 90 degrees. It consists of four members. A plurality of knife edges 26 protrude from the end of the container bottom 22 opposite to the container body 11, and as the container bottom 22 is screwed into the container body 11, the knife edge 26 bites into the annular thin part 21, Eventually, the thin annular portion 21 is adapted to be completely cut. For example, the knife edge 26 may be formed by integrally forming a plurality of protrusions having an inclined sharp tip at the end of the container bottom 22.
[0036]
The temperature sensor 4 is inserted into the container 3 ″ in the same manner as in the above embodiment, but its base end is fixed to the wall plate 1a at the center of the installation base recess by the screw nut 27, and the lead wire 28 of the temperature sensor 4 Passes through the wall plate 1 a and reaches the control unit 10.
[0037]
The time point when the screw ring 23 is attached to the container body 11 may be any time point until the sealed container 3 is finally opened by the knife edge 26 and the sterilized grafted bone is taken out. For example, even when the sealed container 3 ″ is not attached with the screw ring 23, the transplanted bone inside thereof is heat sterilized, and then the screw ring 23 is attached to the sealed container 3 ″ (the right half in FIG. 6). Alternatively, heat sterilization may be performed with the screw ring 23 attached to the sealed container 3 (the left half in FIG. 6). In any case, in the case of the above embodiment, the sealed container could be used repeatedly, but in this embodiment, it is necessary to cut the annular thin portion 21 in order to take out the transplanted bone. Therefore, it can only be used once. Any one of these may be selected according to management requirements. In particular, when it is used only once, it is desirable that the lid 15 be configured so that it cannot be opened again once it is mounted.
[0038]
FIGS. 7 and 8 show a fourth embodiment of the present invention. The illustrated sealed container 30 has a bottomed cylindrical body 31 made of a hard resin material, and its bottom 36 is In the same manner as in the second embodiment, the container body 31 is coupled via the annular thin portion 42. An outer screw 37 is provided on the outer periphery of the bottom portion 36, and is screwed with an inner screw 45 of a screw ring 43 provided with a knife edge 46 similar to the above-described embodiment, and the screw ring 43 is attached.
[0039]
In this embodiment, the lid 32 is attached to the container main body 31 by screwing the outer screw 34 with the inner screw 33 on the container main body 31 side. The lid 32 has a shape that is difficult to grip, and once the lid 32 is attached manually or using a special tool, it cannot be opened again manually. In order to achieve such an object more suitably, the screw ring 43 can be screwed with a normal right screw, whereas the screw of the lid 32 can be a left screw.
[0040]
A collar 38 projects from the center of the inner surface of the bottom 36, and a thermocouple temperature sensor 39 projects from the center. A connector 40 is insert-molded in the bottom portion 36 so that the lead of the temperature sensor 39 passes through the bottom portion 36 and is drawn to the outside. Also in this case, the output of the temperature sensor 39 is irradiated with microwaves in a pulsed manner so as not to be interfered by the current induced by the microwaves, and the output of the temperature sensor is detected only during the pause period of the microwave pulses By doing so, interference due to the current induced by the microwave can be avoided. Alternatively, as indicated by a broken line, the temperature sensor 39 is surrounded by a shield made of a wire mesh 41 electrically connected to the shield of the lead of the temperature sensor 39, and the output of the temperature sensor 39 is induced by microwaves. It is also possible to prevent interference from the generated current. In that case, naturally, it is not necessary to irradiate the microwave in a pulse form or to detect the output of the temperature sensor only in the rest period of the microwave pulse. In the above embodiment, the temperature sensor is used repeatedly, whereas in this embodiment, the temperature sensor 39 is integrated with the container body 31 and used only once.
[0041]
【The invention's effect】
As described above, according to the method for sterilizing a transplanted bone according to the present invention, since it is heated not only from the surface of the transplanted bone but also from the inside of the transplanted bone, it is necessary to raise the temperature to a certain temperature such as 80 ° C. Time can be shortened. In addition, it is possible to computer-control the heating conditions based on the temperature in the heating container that can be easily measured, and by using a program according to the conditions such as the type, size, and shape of the transplanted bone, A wide range of bone grafts can be sterilized efficiently.
[Brief description of the drawings]
FIG. 1 is a front view showing a warming sterilization apparatus suitable for carrying out a bone sterilization method according to the present invention.
FIG. 2 is a longitudinal sectional view showing a first embodiment of a sterile container according to the present invention.
FIG. 3 is a longitudinal sectional view of an essential part showing a state where a rubber cap is attached to the bottom of the sterile container shown in FIG.
FIG. 4 is a view similar to FIG. 2 showing a second embodiment of the sterile container according to the present invention.
FIG. 5 is a cross-sectional view taken along line VV in FIG. 4;
FIG. 6 is a longitudinal sectional view of an essential part showing a third embodiment of a sterile container according to the present invention.
FIG. 7 is a view similar to FIG. 2 showing a fourth embodiment of the sterile container according to the present invention.
FIG. 8 is an exploded sectional view of a fourth embodiment of a sterile container according to the present invention.
[Explanation of symbols]
1 Microwave heating device
1a Wall board
2 Installation stand
2a recess
2b Screwed boss
2c Cap nut
3, 3 ', 3 "aseptic container
4 Temperature sensor
4a lead
5 Reflector
6 Rubber cap
7 Display
8,9 Keyboard
10 Control unit
11 Container body
11a annular groove
12 Seal
13 Cylindrical collar
14 Projection
15 lid
16 colors
17 Concavity and convexity
18, 19 Foamed resin pieces
20 Rubber plug
21 Annular thin part
22 Bottom of container
23 Screw ring
24 External thread
25 Internal thread
26 Knife Edge
27 Screw nut
28 leads
30 containers
31 Container body
32 lids
33, 34 screw
35 seal
36 Bottom
37 screws
38 colors
39 Temperature sensor
40 connectors
41 wire mesh
42 Annular thin part
43 Screw ring
44 screws
45 Internal thread
46 Knife Edge

Claims (3)

A method for heat sterilization of transplanted bone,
Preparing a microwave irradiation device including a microwave irradiation source and a microwave cavity to be irradiated with microwaves by the microwave irradiation source;
A process of preparing a sealed aseptically heated container formed of a material that at least partially transmits microwaves;
Arranging a temperature sensor in the heating container for measuring the temperature of the atmosphere in the heating container;
Placing the grafted bone to be sterilized in the warming container;
Placing the heating container in which the grafted bone is disposed in the microwave cavity, and irradiating the grafted bone with microwaves from the microwave irradiation source;
In the irradiation process, the process of controlling the microwave irradiation amount from the microwave irradiation source according to the output of the temperature sensor,
The heating container is inserted into a collar having a hole communicating with the inside and outside of the container, and an outer end side of the hole of the collar, and the temperature sensor is provided so as to substantially maintain the airtightness of the heating container. A method for warming and sterilizing a transplanted bone, comprising: a self-sealing opening to be received, wherein the temperature sensor projects from the outside of the container into the hole of the collar through the self-sealing opening. .   The first aspect of the present invention is characterized in that the collar has a cylindrical shape, and has protrusions spaced apart from each other in the circumferential direction so as to surround the temperature sensor on the inner end side thereof. The method for warming and sterilizing a transplanted bone according to claim 1.   The method for warming and sterilizing a transplanted bone according to claim 1 or 2, wherein the temperature sensor is surrounded by a shield made of a metal mesh.

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