JP4190846B2 - Corn steep liquor free of precipitates and method for producing the same - Google Patents
Corn steep liquor free of precipitates and method for producing the same Download PDFInfo
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- JP4190846B2 JP4190846B2 JP2002289041A JP2002289041A JP4190846B2 JP 4190846 B2 JP4190846 B2 JP 4190846B2 JP 2002289041 A JP2002289041 A JP 2002289041A JP 2002289041 A JP2002289041 A JP 2002289041A JP 4190846 B2 JP4190846 B2 JP 4190846B2
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- 240000008042 Zea mays Species 0.000 title claims description 26
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims description 26
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims description 26
- 235000005822 corn Nutrition 0.000 title claims description 26
- 238000004519 manufacturing process Methods 0.000 title claims description 10
- 239000002244 precipitate Substances 0.000 title description 26
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 22
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 18
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 18
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 18
- 150000007522 mineralic acids Chemical class 0.000 claims description 15
- 150000007524 organic acids Chemical class 0.000 claims description 15
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 12
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 12
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 11
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 11
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 6
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 6
- 235000011054 acetic acid Nutrition 0.000 claims description 6
- 235000019253 formic acid Nutrition 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 229910017604 nitric acid Inorganic materials 0.000 claims description 6
- 235000006408 oxalic acid Nutrition 0.000 claims description 6
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 5
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 5
- 235000015165 citric acid Nutrition 0.000 claims description 5
- 239000001630 malic acid Substances 0.000 claims description 5
- 235000011090 malic acid Nutrition 0.000 claims description 5
- 235000011007 phosphoric acid Nutrition 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 description 14
- 239000007788 liquid Substances 0.000 description 13
- 238000003860 storage Methods 0.000 description 9
- 238000003756 stirring Methods 0.000 description 7
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 238000009826 distribution Methods 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 235000014655 lactic acid Nutrition 0.000 description 3
- 238000010979 pH adjustment Methods 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 208000003643 Callosities Diseases 0.000 description 2
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Diphosphoinositol tetrakisphosphate Chemical compound OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 229920002261 Corn starch Polymers 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000004845 protein aggregation Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
- Cereal-Derived Products (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Fertilizers (AREA)
Description
【０００１】
【発明の属する技術分野】
本発明は、低温で静置しても沈殿物の発生が無く、流通時および長期にわたり安定した貯蔵を可能とするコーンスチープリカー及びその製造方法に関する。
【０００２】
【従来の技術】
本発明に利用するコーンスチープリカー（CORN STEEP LIQUOR；以下ＣＳＬとする）は、とうもろこし澱粉を製造する際の副産物であって、とうもろこし粒内の水溶性成分を抽出し、これを乳酸醗酵させてから濃縮したものであり、主要な成分として蛋白質、ペプチド、アミノ酸、乳酸、糖分、各種のビタミン及びリン、カリウム、マグネシウム等のミネラルなど多くの成分を多含しており、主にペニシリン醗酵等の微生物工業用培地栄養剤、家畜飼料の栄養成分として、肥料等の原材料に用いられている。
【０００３】
とうもろこしの浸漬液であるＣＳＬは通常、水分４５〜６５％に濃縮されてやや粘性のある液体として製造される。
ＣＳＬは製造された後、貯蔵中に液温が低下したり、また時間が経つと必ず沈殿物が発生し、上澄みの液層と沈殿物層の２層に分離する。
【０００４】
沈殿物の発生する原因は、蛋白質の凝集変性・澱粉の老化・各種塩類の析出等様々あるが、これを解決するために、例えばＣＳＬを保温し、あるいは攪拌維持して沈殿物の発生を防ぐなどが一般におこなわれている。
【０００５】
しかし、保温・攪拌のできない容器での流通および貯蔵時等にはＣＳＬに沈殿物が発生し、使用時等に容器に残った沈殿物の排出等において配管・バルブ等の詰まりを起こし作業性を著しく悪くする。
【０００６】
特にＣＳＬを醗酵培地として使用する場合では、貯槽および醗酵槽までの配管、バルブ、計器などに沈殿物が詰まるなどの問題が生じ、液体肥料に使用した場合では、潅水ホースや噴霧ノズルに沈殿物が詰まり、液体肥料や水の流量管理に重大な悪影響を及ぼすため沈殿物の発生しないＣＳＬの開発が望まれていたが、これらを解決する技術は未だ開発されていないのが現状であった。
【０００７】
上記目的を達成するために、本発明者らは先に遠心分離により沈殿物の少ないＣＳＬおよび製造方法について出願した（特許文献１参照）。この製法によれば、常温下での長期保存を可能とするとともに、取り扱い性にも優れ、貯蔵に際してもかなり長期間に亘って沈殿物の発生が無く安定的に保存できるＣＳＬが得られるというものである。しかしながら、これによって得られるＣＳＬにおいても、特に厳寒地域での使用、たとえば氷点下というような極めて過酷な条件での保管においては沈殿物が発生することがあり長期間の貯蔵における沈殿の発生を防ぐことはできなかった。
【０００８】
この他に、コーンスチープリカーにアルカリを添加して沈殿物を除去する方法もあるが（例えば、特許文献２参照）、アルカリを添加するとコーンスチープリカー中のリン酸分であるフィチンが沈殿し、また蛋白質が、変性・沈殿するなどによりコーンスチープリカーに含まれる多くの有効成分が沈殿として除かれてしまう問題が生じる上、沈殿物の発生も完全に防ぐことはできない。
【０００９】
【特許文献１】
特開２００１−２０４４１０号公報
【特許文献２】
特開昭５３−５４５７４号公報
【００１０】
【発明が解決しようとする課題】
本発明は、低温で静置しても沈殿物の発生が無く、流通時および長期にわたり安定した貯蔵を可能とするコーンスチープリカー及びその製造方法を提供することを目的とする。
【００１１】
【課題を解決するための手段】
本発明者らは、前記の課題を解決するために検討した結果、ＣＳＬに無機酸及び／又は有機酸を添加してｐＨ１〜３に調整したＣＳＬが、冬場の氷点下のような極めて過酷な条件下においても沈殿物の発生が無く、流通時および長期に亘り安定した貯蔵を可能とすることができることを見出し本発明を完成させるに至った。
【００１２】
即ち、本発明は以下の発明を包含する。
（１）コーンスチープリカーに無機酸及び／又は有機酸を添加し、ｐＨ１〜３に調整して得られるコーンスチープリカー。
（２）無機酸及び／又は有機酸が塩酸、硫酸、硝酸、リン酸、酢酸、シュウ酸、クエン酸、リンゴ酸、及び蟻酸からなる群から選択されるものである前記（１）記載のコーンスチープリカー。
（３）コーンスチープリカーに無機酸及び／又は有機酸を添加し、ｐＨ１〜３に調整することを特徴とするコーンスチープリカーの製造方法。
（４）無機酸及び／又は有機酸が塩酸、硫酸、硝酸、リン酸、酢酸、シュウ酸、クエン酸、リンゴ酸、及び蟻酸からなる群から選択されるものである前記（３）記載のコーンスチープリカーの製造方法。
【００１３】
【発明の実施の形態】
以下に、本発明を更に詳しく説明する。
ＣＳＬは、通常、濃縮した形で生産され、ｐＨは約４で固形物を３５〜５０％含有している。その固形物の主成分は、蛋白質・ペプチド・アミノ酸などが２０〜３０％、全糖分が６〜１０％、乳酸１０〜１５％その他灰分が７〜１０％である。
【００１４】
本発明で使用するＣＳＬとしては、とうもろこし浸漬液を濃縮した通常のＣＳＬ、又は予めＣＳＬから静置、遠心分離などで沈殿物を除く処理をしたＣＳＬ等どのようなＣＳＬを用いても良く、特に限定されるものではない。
【００１５】
ＣＳＬに無機酸及び／又は有機酸を添加する方法としては、例えば、撹拌装置の付いた通常のタンクにＣＳＬを入れ、そこに撹拌しながら無機酸及び／又は有機酸を徐々に添加する方法が挙げられる。無機酸及び／又は有機酸を添加する際に発熱する場合もあるので、ＣＳＬの液温をコントロールしながら行うことが好ましい。ｐＨ調整するときのＣＳＬの液温は、ＣＳＬが流動性を有する液温であるかぎり特に限定されないが、通常１０〜７０℃であり、好ましくは２０〜６０℃である。
【００１６】
ｐＨ調整時の撹拌時間は、ＣＳＬのｐＨが１〜３に安定するまで適宜設定する。通常、無機酸及び／又は有機酸添加終了後２０〜３０分間おこなえば充分である。本発明では撹拌終了後のＣＳＬのｐＨを３以下とするが、ｐＨ１〜３が好ましく、ｐＨ１．５〜３が特に好ましい。
なお、ｐＨ調整後にＣＳＬ中に不溶性物質が残存している場合は、フィルター、濾過器、遠心分離などで除去しておくことが好ましい。
【００１７】
本発明で用いられる無機酸としては、塩酸、硫酸、硝酸、リン酸等が用いられ、有機酸としては、酢酸、シュウ酸、クエン酸、リンゴ酸、蟻酸等を用いること
が出来るがこれらに限定されるものではない。
【００１８】
本発明の製造方法により得られるＣＳＬの沈殿重量は、通常１０％以下であり、好ましくは０．１〜１０％、更に好ましくは０．１〜５％である。沈殿重量の値が１０％以下のＣＳＬは、-４℃の低温で且つ攪拌せずに静置した条件下でも沈殿物を発生しない。なお、本明細書でいう沈殿重量(％)は、試料５０ｇをガラス遠沈管に入れて所定温度、例えば室温（２５℃）又は−４℃まで液温を低下させた後、該温度で一定時間、例えば一晩静置して、２０００Ｇの条件で５分間遠心分離して遠沈管に形成された沈殿物重量の試料に対する重量％を表す。
【００１９】
また、本発明の方法と他のＣＳＬ処理手段と組み合わせることにより、より沈殿の少ないＣＳＬを得ることも可能である。例えば、ＣＳＬをデカンター式横型遠心分離機、分離板式竪型遠心分離機等の遠心分離機にかけて遠心分離処理を施してＣＳＬ清澄液を得て、それに無機酸及び／又は有機酸を混合・撹拌してｐＨ３以下に調整する。あるいは、その逆に本発明の方法により得られるＣＳＬに上述のような遠心分離処理を施してもよい。
【００２０】
【実施例】
以下、実施例により本発明を更に具体的に説明するが、本発明の範囲はこれらの実施例に限定されるものではない。なお、以下において％は全て重量基準である。
【００２１】
実施例１
コーンスチープリカー（固形分４５％、ｐＨ３．９）に８５％リン酸を攪拌しながら添加してそれぞれ調整ｐＨの異なるＣＳＬを調製した。調製したＣＳＬ試料５０ｇをガラス遠沈管に入れ、室温（２５℃）、−４℃のそれぞれの温度で１晩静置した後、２０００Ｇの条件で５分間遠心分離した後、遠沈管に形成された沈殿物の試料に対する重量％を沈殿重量とした。得られた沈殿重量の値を表１に示す。
【００２２】
【表１】
上記８５％リン酸でｐＨ１．５〜３に調整したＣＳＬは、２５℃、−４℃のそれぞれの温度で３０日静置しても、沈殿物の増加は見られなかった。これに対し、８５％リン酸無添加ＣＳＬは、沈殿物が増加して１０日後にはＣＳＬの全体が固化した。
【００２４】
実施例２
コーンスチープリカー（固形分４５％、ｐＨ３．９）に、それぞれ８５％リン酸、３５％塩酸、９５％硫酸、６０％硝酸、酢酸、７０％クエン酸、９０％蟻酸、６０％シュウ酸液を攪拌しながら添加してｐＨ３に調整した。
【００２５】
各調製したＣＳＬ試料５０ｇをそれぞれガラス遠沈管に入れ、室温（２５℃）、−４℃で１晩静置した後、２０００Ｇの条件で５分間遠心分離した後、遠沈管に形成された沈殿物の試料に対する重量％を沈殿重量とした。得られた沈殿重量の値を表２に示す。
【００２６】
【表２】
上記各種の酸でｐＨ３に調整したＣＳＬは、２５℃、−４℃で３０日静置しても、沈殿物の増加は見られなかった。これに対し、酸無添加ＣＳＬは、沈殿物が増加して１０日後にはＣＳＬの全体が固化した。
【００２８】
実施例３
コーンスチープリカー（固形分４５％、ｐＨ３．９）をデカンター式横型遠心分離機（三菱化工機(株)製ＫＶＺ２５ＳＬを使用して遠心分離して清澄液を調製した。
このＣＳＬに８５％リン酸を攪拌しながら添加してｐＨ３のＣＳＬを調製し、実施例１と同様に沈殿重量を測定した。その結果を表３に示す。
【００２９】
【表３】
上記デカンター清澄液を８５％リン酸でｐＨ３に調整したＣＳＬは、２５℃、−４℃で３０日静置しても、沈殿物の増加は見られなかった。これに対し、デカンター清澄液、８５％リン酸無添加（原料ＣＳＬ）は沈殿物の増加が見られ、８５％リン酸無添加は１０日後にＣＳＬの全体が固化した。
【００３１】
【発明の効果】
本発明により、低温で静置しても沈殿物の発生がなく、流通時および長期にわたり安定した貯蔵を可能とするコーンスチープリカー及びその製造方法を提供できる。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a corn steep liquor and a method for producing the same, which do not generate precipitates even when allowed to stand at a low temperature and enable stable storage during distribution and for a long period of time.
[0002]
[Prior art]
Corn steep liquor (hereinafter referred to as CSL) used in the present invention is a by-product in producing corn starch, and after extracting water-soluble components in corn grains and subjecting it to lactic acid fermentation Concentrated and contains many components such as protein, peptide, amino acid, lactic acid, sugar, various vitamins and minerals such as phosphorus, potassium and magnesium as the main components, mainly microorganisms such as penicillin fermentation It is used for raw materials such as fertilizers as nutrients for industrial medium nutrients and livestock feed.
[0003]
CSL, which is a corn dipping solution, is usually produced as a slightly viscous liquid that is concentrated to a moisture content of 45-65%.
After the CSL is manufactured, the liquid temperature is lowered during storage, or a precipitate is always generated over time, and is separated into a supernatant liquid layer and a precipitate layer.
[0004]
There are various causes of precipitates, such as protein aggregation and denaturation, starch aging, precipitation of various salts, etc. In order to solve this, for example, CSL is kept warm or stirred to prevent the formation of precipitates. Etc. are generally done.
[0005]
However, precipitates are generated in the CSL during distribution and storage in containers that cannot be kept warm or stirred, causing clogging of piping, valves, etc. during discharge of precipitates remaining in the containers, etc. Make it extremely bad.
[0006]
In particular, when CSL is used as a fermentation medium, problems such as clogging of sediments occur in piping, valves, instruments, etc. to storage tanks and fermentation tanks. However, the development of CSL that does not generate precipitates has been desired because it has a serious adverse effect on the flow rate control of liquid fertilizer and water, but the technology to solve these problems has not been developed yet.
[0007]
In order to achieve the above object, the present inventors previously filed an application for CSL and a production method with less precipitate by centrifugation (see Patent Document 1). According to this production method, a CSL can be obtained that can be stored for a long period of time at room temperature, has excellent handleability, and can be stored stably without generation of precipitates for a long period of time during storage. It is. However, even in the CSL obtained by this, precipitates may be generated particularly in use in extremely cold regions, for example, storage under extremely harsh conditions such as below freezing point, thus preventing precipitation during long-term storage. I couldn't.
[0008]
In addition to this, there is a method of removing the precipitate by adding alkali to corn steep liquor (see, for example, Patent Document 2), but when alkali is added, phytin, which is the phosphoric acid content in corn steep liquor, is precipitated, In addition, there is a problem that many active ingredients contained in the corn steep liquor are removed as precipitates due to protein denaturation / precipitation, and the generation of precipitates cannot be completely prevented.
[0009]
[Patent Document 1]
Japanese Patent Laid-Open No. 2001-204410 [Patent Document 2]
JP-A-53-54574
[Problems to be solved by the invention]
An object of the present invention is to provide a corn steep liquor and a method for producing the same, which do not generate precipitates even when allowed to stand at a low temperature and enable stable storage during distribution and for a long period of time.
[0011]
[Means for Solving the Problems]
As a result of investigations to solve the above-mentioned problems, the present inventors have found that CSL adjusted to pH 1 to 3 by adding an inorganic acid and / or organic acid to CSL has extremely severe conditions such as below freezing in winter. The inventors have found that there is no generation of precipitates even underneath, and that stable storage can be performed during distribution and for a long time, and the present invention has been completed.
[0012]
That is, the present invention includes the following inventions.
(1) Corn steep liquor obtained by adding an inorganic acid and / or organic acid to corn steep liquor and adjusting the pH to 1 to 3.
(2) Corns according to (1), wherein the inorganic acid and / or organic acid is selected from the group consisting of hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, acetic acid, oxalic acid, citric acid, malic acid, and formic acid. Cheap car.
(3) A method for producing corn steep liquor, comprising adding an inorganic acid and / or an organic acid to corn steep liquor and adjusting the pH to 1 to 3.
(4) Corns according to (3), wherein the inorganic acid and / or organic acid is selected from the group consisting of hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, acetic acid, oxalic acid, citric acid, malic acid, and formic acid. A manufacturing method of cheap cars.
[0013]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, the present invention will be described in more detail.
CSL is usually produced in a concentrated form, having a pH of about 4 and containing 35-50% solids. The main components of the solid are 20-30% protein, peptide, amino acid, etc., 6-10% total sugar, 10-15% lactic acid, and 7-10% other ash.
[0014]
As the CSL used in the present invention, any CSL such as normal CSL in which the corn immersion liquid is concentrated, or CSL that has been treated in advance by standing, centrifugation, etc. from the CSL may be used. It is not limited.
[0015]
As a method of adding inorganic acid and / or organic acid to CSL, for example, there is a method in which CSL is put in a normal tank equipped with a stirrer and inorganic acid and / or organic acid is gradually added while stirring. Can be mentioned. Since heat may be generated when an inorganic acid and / or an organic acid is added, it is preferable to carry out while controlling the liquid temperature of CSL. Although the liquid temperature of CSL at the time of pH adjustment is not specifically limited as long as CSL is the liquid temperature which has fluidity | liquidity, it is 10-70 degreeC normally, Preferably it is 20-60 degreeC.
[0016]
The stirring time at the time of pH adjustment is appropriately set until the pH of the CSL is stabilized at 1 to 3. Usually, it is sufficient to carry out for 20 to 30 minutes after the addition of the inorganic acid and / or organic acid. In this invention, although pH of CSL after completion | finish of stirring shall be 3 or less, pH 1-3 is preferable and pH 1.5-3 are especially preferable.
In addition, when insoluble substances remain in the CSL after pH adjustment, it is preferably removed by a filter, a filter, a centrifuge, or the like.
[0017]
As the inorganic acid used in the present invention, hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid and the like are used, and as the organic acid, acetic acid, oxalic acid, citric acid, malic acid, formic acid and the like can be used, but are not limited thereto. Is not to be done.
[0018]
The precipitation weight of CSL obtained by the production method of the present invention is usually 10% or less, preferably 0.1 to 10%, more preferably 0.1 to 5%. CSL having a precipitation weight value of 10% or less does not generate a precipitate even under a low temperature of −4 ° C. and standing without stirring. In this specification, the precipitation weight (%) is obtained by putting 50 g of a sample in a glass centrifuge tube and lowering the liquid temperature to a predetermined temperature, for example, room temperature (25 ° C.) or −4 ° C. For example, it represents the weight% of the weight of the precipitate formed in the centrifuge tube after standing overnight and centrifuging for 5 minutes under the condition of 2000 G.
[0019]
Further, by combining the method of the present invention with other CSL processing means, it is also possible to obtain CSL with less precipitation. For example, CSL is applied to a centrifugal separator such as a decanter horizontal centrifuge or a separator plate vertical centrifuge to give a CSL clarified liquid, which is then mixed and stirred with an inorganic acid and / or an organic acid. To adjust the pH to 3 or less. Alternatively, conversely, the CSL obtained by the method of the present invention may be subjected to the centrifugation treatment as described above.
[0020]
【Example】
EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples. However, the scope of the present invention is not limited to these examples. In the following, all percentages are based on weight.
[0021]
Example 1
CSL having a different adjusted pH was prepared by adding 85% phosphoric acid to corn steep liquor (solid content 45%, pH 3.9) with stirring. 50 g of the prepared CSL sample was put in a glass centrifuge tube, allowed to stand overnight at room temperature (25 ° C.) and −4 ° C., and then centrifuged at 2000 G for 5 minutes, and then formed into a centrifuge tube. The weight% of the precipitate relative to the sample was defined as the precipitation weight. The obtained precipitation weight values are shown in Table 1.
[0022]
[Table 1]
The CSL adjusted to pH 1.5-3 with 85% phosphoric acid did not show an increase in precipitate even when allowed to stand at 25 ° C. and −4 ° C. for 30 days. In contrast, 85% phosphoric acid-free CSL solidified the entire CSL 10 days after the precipitation increased.
[0024]
Example 2
Corn steep liquor (solid content 45%, pH 3.9), 85% phosphoric acid, 35% hydrochloric acid, 95% sulfuric acid, 60% nitric acid, acetic acid, 70% citric acid, 90% formic acid, 60% oxalic acid solution, respectively The pH was adjusted to 3 by adding with stirring.
[0025]
50 g of each prepared CSL sample was placed in a glass centrifuge tube, allowed to stand at room temperature (25 ° C.) and −4 ° C. overnight, centrifuged at 2000 G for 5 minutes, and then a precipitate formed in the centrifuge tube. % By weight of the sample was defined as the precipitation weight. The obtained precipitation weight values are shown in Table 2.
[0026]
[Table 2]
The CSL adjusted to pH 3 with the above-mentioned various acids did not show an increase in precipitate even when allowed to stand at 25 ° C. and −4 ° C. for 30 days. On the other hand, the acid-free CSL solidified the entire CSL 10 days after the precipitation increased.
[0028]
Example 3
Corn steep liquor (solid content 45%, pH 3.9) was centrifuged using a decanter horizontal centrifuge (KVZ25SL, manufactured by Mitsubishi Chemical Corporation) to prepare a clarified liquid.
85% phosphoric acid was added to this CSL with stirring to prepare CSL having a pH of 3, and the precipitation weight was measured in the same manner as in Example 1. The results are shown in Table 3.
[0029]
[Table 3]
CSL prepared by adjusting the decanter clarified liquid to pH 3 with 85% phosphoric acid did not show an increase in precipitate even when allowed to stand at 25 ° C. and −4 ° C. for 30 days. On the other hand, the decanter clarified liquid and 85% phosphoric acid-free addition (raw material CSL) showed an increase in precipitates, and 85% phosphoric acid-free addition solidified the entire CSL after 10 days.
[0031]
【The invention's effect】
According to the present invention, it is possible to provide a corn steep liquor and a method for producing the same that can be stably stored during distribution and for a long period of time without generation of precipitates even when left at low temperature.
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| JP2016032444A (en) * | 2014-07-31 | 2016-03-10 | 王子コーンスターチ株式会社 | Clear corn steep liquor and production method thereof |
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