JP4193218B2 - Biotin dissolution method and biotin-containing internal solution - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a method for dissolving biotin and an internal solution containing biotin.
[0002]
[Prior art]
Biotin is also called vitamin H and is a vitamin necessary for maintaining normal skin and hair. In the acidic range (2 to 5), which is the pH range of general drinks, biotin is hardly soluble in water, and some contrivance is required for its dissolution.
Conventional methods for dissolving biotin include a method using polyvinyl pyrrolidone (Japanese Patent Laid-Open No. 7-76520) and a method of dissolving biotin after adjusting to neutral to alkaline using alkali hydroxide or the like. ing.
However, polyvinyl pyrrolidone may react with a coexisting component to produce a precipitate, or may react with other ingredients such as vitamins and amino acids to lower the component. In addition, the method using alkali hydroxide or the like is neutral to alkaline, and even if biotin is dissolved, if it is returned to the acidic range that is the pH of the internal solution, biotin may precipitate over time ( JP-A-7-76520). Even if it does not precipitate, it is not necessarily a preferable method, for example, it is necessary to add a component that is not necessary for medicinal effects, and the preparation becomes complicated.
[0003]
[Problems to be solved by the invention]
An object of the present invention is to provide a method for rapidly and easily dissolving biotin in water without adding unnecessary components in the preparation and an internal solution containing biotin.
[0004]
[Means for Solving the Problems]
As a result of diligent research on the above object, the present inventors have found that when biotin is separately dissolved in an aqueous solution of an amino acid salt having a buffering capacity, the intended object is achieved, and the present invention has been completed.
That is, the present invention is a biotin dissolution method characterized by dissolving biotin in an aqueous solution of an amino acid salt having a buffering capacity. Another aspect of the present invention is an internal solution containing biotin and an amino acid salt having a buffering capacity and having a pH in the range of 2 to 5. Another aspect of the present invention is an internal liquid solution containing biotin and an amino acid salt having a buffering capacity for dissolving biotin.
[0005]
DETAILED DESCRIPTION OF THE INVENTION
Examples of the amino acid salt having a buffering capacity in the present invention include aspartate. Aspartate is one or a combination of magnesium and potassium aspartate, magnesium aspartate, and potassium aspartate, with magnesium and potassium aspartate being particularly preferred.
[0006]
The method for dissolving biotin of the present invention is achieved by dissolving amino acids having a buffer capacity in water and then dissolving biotin. In addition, the biotin-containing oral solution of the present invention is prepared by first dissolving biotin in an aqueous solution of amino acids having a buffering capacity as described above, separately preparing a solution in which various compounding components are dissolved, mixing both solutions, and then necessary. It is manufactured by adjusting the pH accordingly.
[0007]
The concentration of the amino acid salt having a buffering capacity for dissolving biotin varies depending on pH and the like. For example, in order to dissolve 0.2 mg of biotin, the aqueous solution is usually 50 to 500 mg, preferably 100 to 300 mg dissolved in 100 ml of water. 50-100 ml is used. Hereinafter, the amount of an aqueous solution of an amino acid salt having a buffer capacity is determined by the amount of biotin dissolved.
[0008]
Furthermore, the internal liquid preparation of the present invention includes, in addition to the above components, compounding ingredients that can be used in normal internal liquid preparations, such as various vitamins (for example, vitamin A, vitamin C, vitamin D, vitamin E, vitamin P, or salts thereof). , Esters or derivatives), amino acids (eg, taurine, L-aspartic acid, L-arginine, tryptophan, lysine, etc.), herbal medicines (eg, muirapuama, carrot, diau, etc.), caffeine, royal jelly, polyhydric alcohols (eg, propylene glycol) Etc.), organic acids (eg, citric acid, malic acid, lactic acid, succinic acid, etc.), mineral components (eg, calcium gluconate, calcium lactate, ammonium iron citrate, etc.), perfumes, preservatives, etc. It can mix | blend in the range which is not.
[0009]
【The invention's effect】
According to the present invention, a biotin-containing internal medicine that provides a quick and easy dissolution method without adding unnecessary components for the dissolution of biotin, is stable over time in the acidic region, and can be replenished with various components. A solution was provided.
[0010]
【Example】
Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples.
Example 1
Taurine 2000mg
Biotin 200μg
Magnesium and potassium aspartate 200mg
Maltitol 1500mg
Vitamin B6 5mg
Citric acid (pH adjuster) appropriate amount sodium citrate 10mg
Sodium benzoate 70mg
Stevia extract 30mg
Fragrance Trace purified water 100ml (pH3.5)
After dissolving magnesium potassium aspartate in 80 ml of purified water, biotin was added and dissolved while stirring. After confirming that taurine, maltitol, vitamin B6, sodium citrate, sodium benzoate, stevia extract, and fragrance were added and dissolved in that order, purified water was added to adjust the total volume to 100 ml, and citric acid was added. By adjusting the pH to 3.5, an internal solution was obtained.
[0011]
Example 2
Taurine 1000mg
Biotin 200μg
Magnesium aspartate 200mg
Vitamin B2 5mg
Sorbitol 2000mg
Maltitol 9000mg
Malic acid 100mg
Citric acid (pH adjuster) appropriate amount Sodium benzoate 60mg
Stevia extract 25mg
Fragrance Micropurified water 100ml (pH 2.8)
About each said component, the internal liquid preparation for nourishing tonic was obtained by the same method as Example 1.
[0012]
Example 3
Taurine 1000mg
Biotin 200μg
Magnesium and potassium aspartate 200mg
Vitamin B1 nitrate 2mg
Erythritol 5000mg
Malic acid 100mg
Citric acid (pH adjuster) appropriate amount nipabuchi 4mg
Stevia extract 15mg
Purified water 100ml (pH 4.0)
About each said component, the internal liquid preparation for nourishing tonic was obtained by the same method as Example 1.
[0013]
Test example [Solubility test]
{Circle around (1)} In Example 1, 200 mg of biotin was added after dissolving 200 mg of magnesium / potassium aspartate in 80 ml of purified water and stirred at room temperature, and dissolution of biotin was confirmed after 10 minutes. To this, other components in the formulation were dissolved and the total amount was adjusted to 100 ml with purified water, and then citric acid was added to adjust the pH to 3.5. As a result of storage at 5 ° C. for 1 week, biotin precipitation was not observed.
[0014]
{Circle around (2)} In Example 2, 200 mg of biotin was added with stirring at room temperature after dissolving 200 mg of magnesium aspartate in 80 ml of purified water, and dissolution of biotin was confirmed after 10 minutes. This was adjusted in the same manner as in (1) and stored at 5 ° C. for 1 week. As a result, no biotin precipitation was observed.
[0015]
(3) When 200 μg of biotin was added to 80 ml of purified water at room temperature and dissolution was attempted with stirring, it was not dissolved after 60 minutes.
[0016]
(4) 200 μg of biotin was added to 80 ml of purified water maintained at 80 to 90 ° C. with stirring, and dissolution was attempted. However, dissolution was not observed after 60 minutes.
[0017]
(5) After dissolving 10 mg of sodium citrate in 80 ml of purified water and adding 200 μg of biotin with stirring, dissolution was attempted, but after 60 minutes, it was not dissolved.
[0018]
(6) After dissolving 10 mg of sodium citrate in 80 ml of purified water maintained at 80 to 90 ° C., 200 μg of biotin was added with stirring, and dissolution was attempted.

Claims (6)

A method for dissolving biotin, comprising dissolving biotin in an aqueous solution of aspartate . Magnesium potassium aspartate aspartic acid, dissolution process of claim 1 wherein at least one of magnesium aspartate, and potassium aspartate. An internal solution containing biotin and aspartate and having a pH in the range of 2 to 5. An oral solution characterized in that biotin is dissolved in an aqueous solution of aspartate and the pH is in the range of 2-5. An internal solution containing aspartate for dissolving biotin and biotin. The oral solution according to any one of claims 3 to 5, wherein the aspartate is one or more of magnesium and potassium aspartate, magnesium aspartate and potassium aspartate.