JP4252383B2 - Diluent for electrolyte analyzer with improved storage stability - Google Patents

Description

【００２８】
結果
【００２９】
希釈液に抗菌剤であるパラホルムアルデヒドのみを添加したものは、無関係塩である硫酸マグネシウムを追加したものと比較し、クロライド電極のスロープが1ヶ月で8.1％、5ヶ月で11.1％低下した。抗菌剤だけでは効果が経時的に減少するが、無関係塩を追加することにより劣化を遅らせることが可能となった。
【００３０】
【発明の効果】
以上のように、本発明によれば抗菌剤を含む分析装置用希釈液に測定項目ではないイオンからなる無関係塩を添加することにより、抗菌剤のみでは十分な抗菌作用を得られない希釈液の保存性を向上させることができる。また、抗菌剤と無関係塩を組み合わせて各々の添加量を最小限に抑えれば、抗菌剤による電極へのストレスや希釈液の変色、希釈液成分の組成変化を抑えることもできる。加えて、無関係塩は抗菌剤よりも安価で安全性も高い場合が多いので汎用性がある。
【図面の簡単な説明】
【図１】 本発明の希釈液を使用する際に用いた測定装置の構成を示す図である。
【符号の説明】
１・・・希釈液
２・・・電圧計
３・・・参照電極
４・・・電極
５・・・希釈液または校正液
６・・・測定セル[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a diluent for a dilution method electrolyte analyzer.
[0002]
[Prior art]
When bacteria are propagated in the dilution solution for the dilution method electrolyte analyzer, the metabolite affects the normal measurement of chloride ions. This is because the chloride electrode using a quaternary ammonium salt as a sensitive substance has low ion selectivity. In order to suppress the deterioration of the diluted solution and improve the storage stability, an antibacterial agent must be used to suppress the growth of bacteria.
[0003]
[Problems to be solved by the invention]
However, it is difficult to search for an antibacterial agent exhibiting sufficient antibacterial action without interfering with normal measurement with an ion-selective electrode, and to control the residual concentration of the antibacterial agent in order to maintain the antibacterial effect for a long time.
[0004]
[Means for Solving the Problems]
The present invention solves the above problem by suppressing the growth of bacteria by adding an irrelevant salt consisting of ions that are not measurement items to a diluting solution that cannot obtain a sufficient antibacterial effect with an antibacterial agent alone, and adjusting the salt concentration. Is.
[0005]
【Example】
The raw materials and equipment used at the beginning are described.
[0006]
Sodium chloride Wako Pure Chemical Industries, Ltd.
[0007]
Potassium chloride Wako Pure Chemical Industries, Ltd.
[0008]
Lithium chloride Wako Pure Chemical Industries, Ltd.
[0009]
Sulfuric acid Wako Pure Chemical Industries, Ltd.
[0010]
2,2 ', 2''-nitrilotriethanol (triethanolamine) Pure Chemicals Model No. 49135-1263
[0011]
Paraformaldehyde 95% made by Merck Japan KK
[0012]
Magnesium sulfate heptahydrate Wako Pure Chemical Industries, Ltd. Model No. 131-00405
[0013]
NUC-silicone (L-7604) made by Nippon Unica KK Model No.B27070192-04696
[0014]
As the water, ultrapure water supplied from an ultrapure water device manufactured by Millipore was used.
[0015]
In addition, a measuring flask, a medicine bowl, a magnetic stirrer, a medicine wrapping paper, an electronic balance, etc. were used as equipment.
[0016]
Preparation method [0017]
Sodium chloride 0.5844g, potassium chloride 0.07455g, lithium chloride 0.1696g, sulfuric acid 3.727g, 2,2 ', 2''-nitrilotriethanol (triethanolamine) 14.919g, paraformaldehyde 5g, magnesium sulfate heptahydrate 4.9296g Were weighed and dissolved in pure water, and the volume was increased in a 5-liter volumetric flask. After the prepared aqueous solution was filled in a bottle, 15 drops of NUC-silicone was added and stirred to obtain a diluent.
[0018]
Experimental example [0019]
Dilution storage tests were conducted. The measurement apparatus having the configuration shown in FIG. 1 was loaded with the diluent of the example and calibrated. The control was a dilution without addition of magnesium sulfate.
[0020]
Reagents used [0021]
Calibration solution for measurement 1 25.1292 g of sodium chloride, 1.4910 g of potassium chloride, 15.6156 g of disodium hydrogen phosphate, 1.44 g of acetic acid and 3.5 g of paraformaldehyde were dissolved in 5 liters of pure water.
[0022]
Calibration solution 2 for measurement 45.5832 g of sodium chloride, 16.4010 g of potassium chloride, 15.6156 g of disodium hydrogen phosphate, 1.44 g of acetic acid, and 3.5 g of paraformaldehyde were dissolved in 5 liters of pure water.
[0023]
Experimental method [0024]
During storage, close the lid of the bottle containing the diluent and store at room temperature. Calibrate with the instrument before and after storage to check the slope change.
[0025]
Results [0026]
The results of the storage test are shown in Table 1. In addition, a numerical value is the ratio of the electrode slope of a control dilution with respect to the electrode slope of an Example dilution.
[0027]
[Table 1]

[0028]
Result [0029]
Compared with the addition of the antibacterial agent paraformaldehyde to the diluted solution, the chloride electrode slope decreased 8.1% in 1 month and 11.1% in 5 months compared to the addition of magnesium sulfate, an unrelated salt. The effect of the antibacterial agent alone decreases over time, but the addition of an irrelevant salt has made it possible to delay the deterioration.
[0030]
【The invention's effect】
As described above, according to the present invention, by adding an irrelevant salt composed of ions that are not measurement items to a diluent for an analyzer containing an antibacterial agent, a dilute solution that cannot obtain a sufficient antibacterial action only by the antibacterial agent Preservability can be improved. In addition, if the addition amount of each of the antibacterial agent and the irrelevant salt is minimized, the stress on the electrode due to the antibacterial agent, the color change of the diluent, and the composition change of the diluent component can be suppressed. In addition, unrelated salts are versatile because they are often cheaper and safer than antibacterial agents.
[Brief description of the drawings]
FIG. 1 is a diagram showing a configuration of a measuring apparatus used when using a diluent of the present invention.
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 1 ... Dilution liquid 2 ... Voltmeter 3 ... Reference electrode 4 ... Electrode 5 ... Dilution liquid or calibration liquid 6 ... Measurement cell

Claims (1)

In a dilute solution for a dilution method electrolyte analyzer , which includes measurement items of sodium ion, potassium ion and chloride ion with an ion selective electrode, the sensitive substance of the chloride ion selective electrode is a quaternary ammonium salt, and an antibacterial agent , A diluent for a dilution method electrolyte analyzer , wherein magnesium sulfate is added to the diluent as an irrelevant salt composed of ions that are not measurement items, and the salt concentration is adjusted to improve storage stability.

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