JP4452578B2 - Skin external composition - Google Patents

Description

  The present invention relates to a composition for external use on skin and a food, and in particular, contains an extract obtained from Nejirefama beans fruit, in particular, an extract obtained using water, a water-soluble organic solvent or a mixed solvent thereof as an extraction solvent. The present invention relates to a composition for external use on skin and food excellent in the effect of improving rough skin caused by ultraviolet rays and the like.

  The human body is affected in various ways by exposure to ultraviolet rays. When active oxygen, lipid peroxide, and the like are generated, they cause inflammation and cause great damage to tissues such as skin. Further, these inflammations and skin damage cause rough skin, which causes loss of moisture, gloss and texture of the skin, and when the effect reaches the dermis, it causes photoaging such as wrinkles. In addition, active oxygen generated by ultraviolet rays and various factors released from epidermal cells activate tyrosinase of melanocytes and overproduce melanin. Excess melanin is delivered to epidermal cells, changing the skin tone and leading to skin darkening. Other ultraviolet rays may cause skin cancer through DNA damage.

  For the purpose of preventing and improving various skin problems such as rough skin, photoaging, pigmentation, etc. caused by ultraviolet rays, conventionally, cosmetics, pharmaceuticals, foods, etc. have been used for dibutylhydroxytoluene (BHT), butylhydroxyanisole. Synthetic antioxidants such as (BHA) and various antioxidants such as naturally derived α-tocopherol and ascorbic acid are used (for example, see Patent Documents 1 and 2). However, the effect of preventing and improving skin troubles caused by exposure to ultraviolet rays is not sufficient, and there is a current situation that the antioxidant property does not necessarily lead to improvement of skin troubles caused by ultraviolet rays. Furthermore, the former synthetic antioxidant has a concern in terms of safety, and the latter has many problems in terms of stability.

  In addition, various antioxidant substances extracted from plant seeds have been manufactured and tried to be blended into cosmetics, pharmaceuticals, foods, etc. When using plants as raw materials, procurement of the raw materials, production costs, etc. From the standpoint of production, the production amount is limited, and the production cost is expensive. Therefore, it is difficult to blend an effective amount substantially.

JP-A-5-320036 JP-A-9-263513

  Under such circumstances, the object of the present invention is to provide a safe, stable and inexpensive composition for external use of skin and food that is excellent in improving rough skin caused by an oxidative reaction, an inflammatory reaction, etc. caused by ultraviolet rays. There is to do.

As a result of intensive studies in view of the above circumstances, the present inventors have found that the extract obtained from the fruit of Nejirefama beans, that is, the beans that are seeds and the pods that are the skin and flesh, has an antioxidant effect, Various active ingredients having an inflammatory effect are contained, and when the extract was blended into a composition for external use of skin or food, it was found that it has a remarkable effect for improving rough skin caused by ultraviolet rays. Moreover, they are naturally derived substances, have no safety problems, and have excellent stability.

  That is, the first invention of the present application lies in an external composition for skin characterized by blending an extract obtained by solvent extraction from Nejirefamame fruit. 2nd invention exists in said skin external composition characterized by the extraction solvent being chosen from water, a water-soluble organic solvent, or these mixed solvents. A third aspect of the present invention is a food product characterized by blending an extract obtained by solvent extraction from Nejirefama beans fruit. 4th invention exists in said foodstuff whose extraction solvent is chosen from water, a water-soluble organic solvent, or these mixed solvents.

  In the present invention, by blending an extract obtained from the torrefi bean fruit into a composition for external use of skin or food, it is safe, stable and excellent in preventing rough skin caused by ultraviolet rays and the like. An inexpensive composition for external use on skin and food can be provided.

Hereinafter, embodiments of the present invention will be described in detail. Nephirus bean ( Parkia speciosa ) is a legume plant native to Malaysia, distributed from Malaysia to Indonesia. Usually, seed beans are eaten raw or in a stir-fry food, and young fruits such as strawberry and beans are raw as cocoon beans, or sliced and eaten in boiled or curry. In other words, it is treated as an ordinary vegetable and is inexpensive. In the present invention, the fruit (bean and / or koji) of the screw reef bean is used as the extraction material. The fruit can be either mature or immature. Further, it may be either a fresh product or a processed product such as a dried product.

  In the present invention, the extraction solvent used for solvent extraction can be any known extraction solvent used for solvent extraction from plant materials. As the extraction solvent, a solvent having a relatively high hydrophilicity is preferably used because an effective extract meeting the purpose of the present invention can be obtained. As such a solvent, in the present invention, a solvent selected from water, a water-soluble organic solvent or a mixed solvent thereof is particularly preferably used. The water-soluble organic solvent refers to a solvent having some compatibility with water, specifically, lower alcohols such as methanol, ethanol, propanol, isopropanol, butanol, polyhydric alcohols such as butylene glycol, propylene glycol, Examples thereof include aliphatic carboxylic acid esters such as methyl acetate and ethyl acetate, and other relatively polar organic solvents such as acetone. Among the extraction solvents, an ethanol aqueous solution is preferable.

  For extraction, first, the torrefi bean fruit is shredded and crushed by a known appropriate means. Extraction with a solvent may be performed according to a known method. For example, when water, a water-soluble organic solvent, or a mixed solvent thereof is used as an extraction solvent, the extraction solvent amount is 1 to 50 times, preferably 3 to 10 times in weight ratio to the extraction raw material, and 0 ° C. to Extraction may be performed for 0.1 to 50 hours, preferably 0.5 to 24 hours, at a temperature in the range of 100 ° C. and lower than the boiling point of the extraction solvent. Although the extraction may be in a stationary state, it is desirable to perform the extraction with moderate stirring for more efficient extraction.

  The Nerefu pea fruit extract obtained in this way may be blended in the composition for external use of skin or food as it is, or may be blended after being concentrated or dried. Moreover, you may use what re-extracted what was extracted with the other organic solvent etc. Furthermore, a fraction obtained by fractionating and purifying the extract by a known fractionation means or purification means may be used.

The composition for external use on skin and food blended with the extract of the screw reef pea fruit of the present invention can be various pharmaceuticals, quasi drugs, cosmetics, foods, health foods and the like. The dosage form is not particularly limited, and various forms can be used. Specifically, ointments, lotions, emulsions, creams, packs, granules, cataplasms, and the like can be used. Cosmetics are not particularly limited as long as they are applied to the human skin, and are cosmetics such as skin cosmetics, foundations, makeup cosmetics such as lipsticks, hair cosmetics such as shampoos, rinses and treatments, bath preparations, facial cleansers. It is possible to use various things. In addition, the food can be in various forms such as tablets, pills, dragees, powders, granules, teas, tea bags, hard capsules, soft capsules, strawberries, ice, baked goods.

  The compounding amount of the torrefi bean fruit extract to the composition for external use of the skin and food according to the present invention is not particularly limited, and since it varies depending on the purpose and target, it cannot be defined unconditionally. However, it is generally 0.00001 to 5.0% by mass, preferably 0.001 to 1.0% by mass in terms of dry matter.

  Moreover, the composition for external use and the food of the present invention can be combined with various components that are usually used within a range that does not impair the effect, if necessary. For example, in the case of cosmetics, oils, surfactants, humectants, thickeners, preservatives, antioxidants, ultraviolet absorbers, ultraviolet scattering agents, fragrances, dyes, pigments and the like. In the case of food, it is an excipient, additive, binder, thickener, emulsifier, colorant, fragrance, food additive, seasoning and the like.

  EXAMPLES Hereinafter, although this invention is explained in full detail based on an Example and a comparative example, this invention is not limited by these. Moreover, unless otherwise specified,% shown in an Example and a comparative example means the mass%.

Production example (Nedrafu bean fruit extract)
The surface of 1023 g of immature screw reef peas containing seeds and straw was washed and crushed with a knife and a mixer. Crushing with a mixer was performed by adding 10% amount of 50% ethanol aqueous solution to the fruit weight. The crushed product was concentrated with an evaporator and then dried using a freeze dryer to obtain 120.8 g of crushed and dried product. To 100 g of the crushed and dried product, 1000 mL of 50% aqueous ethanol solution was added, stirred at room temperature for 24 hours, and filtered to obtain a torrefi bean fruit extract (518 g, 41.57 g in terms of solid content).

[Measurement of antioxidant power]
In accordance with the following method, the antioxidant power of the torrefi bean fruit extract was measured.
Solution A: 0.5 mmol / L DPPH (1,1-Diphenyl-2-picrylhydrazyl, Wako Pure Chemical Industries) ethanol solution.
Liquid B: 100 mmol / L Tris-HCl aqueous solution (pH 7.4).
Liquid C (sample): Nejirefamame fruit extract of production example.
Procedure: Take 1.0 mL of solution A, 0.8 mL of solution B, and 0.2 mL of solution C in a test tube, and stir with a vortex mixer for about 10 seconds. Absorbance A ₅₁₇ at ₅₁₇ nm was measured. As a control, the same amount of 75% ethanol solution was added instead of liquid C, and the same amount of ethanol was added instead of liquid A for the purpose of correcting the absorbance derived from the measurement sample itself, The antioxidant power value was determined according to the following equation. As a comparison of the antioxidant power, a 1 mmol / L ascorbic acid solution prepared with a 75% ethanol solution was used instead of the C solution.
Formula: [A ₅₁₇ (control)-{A ₅₁₇ (sample) -A ₅₁₇ (correction)}] / A ₅₁₇ (control) × 100 (%)

The measurement results are shown in Table 1. The antioxidative activity of the extract of the twisted bean fruit of the present invention was comparable to that of ascorbic acid.

[Table 1]
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Sample Antioxidant power (%)
――――――――――――――――――――――――
Nejirefamame fruit extract 90.69
Ascorbic acid (control) 75.99
――――――――――――――――――――――――

Example 1 [Rough skin improvement effect]
The effect of the composition for external use on skin containing ultraviolet rays of the Nephirefus saccharum fruit extract on rough skin was evaluated by the following test using hairless mice. The animals used were 5 hairless mice per group, 9 weeks old at the start of the test.

1. Ultraviolet irradiation and rough skin evaluation method Ultraviolet irradiation was performed by irradiating UVB region ultraviolet rays in an amount of about 7 times the minimum amount of erythema. The degree of rough skin caused by ultraviolet irradiation was evaluated by the fluctuation of transdermal moisture transpiration (TEWL). TEWL was measured using a hydrograph before sample application, before ultraviolet irradiation, after 3 days and after 4 days of irradiation. A value obtained by dividing the values after 3 days and 4 days after irradiation by the value before the sample application was calculated as the TEWL fluctuation rate, and this value was used as an index of the degree of rough skin.
2. Sample and Experimental Method A sample (Example 1) was prepared using a 50% ethanol aqueous solution as a base and blended with a 1% concentration of the Nervous bean fruit extract obtained in the production example. First, 0.05 mL of this sample was applied to the skin of the back test site of a hairless mouse once a day for 7 days (preliminary application). The day after the final pre-application, UV irradiation was performed, and the sample was also applied the next day after irradiation and 2 days after the irradiation. Table 2 shows the effect of the application of the sample on the rough skin of the hairless mouse. A sample containing only the base was used as Comparative Example 1.

[Table 2]
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Sample TEWL fluctuation rate ―――――――――――――――――――――――――――――――――――
Example 1 (Nedrafu bean fruit extract 1.0%) 3.52 (3 days after irradiation)
2.86 (4 days after irradiation)
Comparative Example 1 (base only) 7.27 (3 days after irradiation)
8.17 (4 days after irradiation)
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  In this test system, the smaller the TEWL fluctuation rate value, the more the UV rough skin is suppressed. From the comparison with the comparative example of this test, it can be seen that rough skin due to ultraviolet rays is improved by applying the composition for external application containing the torrefi bean extract. In addition, the fluctuation | variation of TEWL by prior application was not seen.

Example 2 [Evaluation of safety and stability]
A lotion of Example 2 was prepared with the composition shown in Table 3 below, and applied as a test sample to human skin continuously for 1 month.

[Table 3]
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Ingredient name Compounding amount ――――――――――――――――――――――
Ethanol 5.0
Polyoxyethylene monolaurate 0.3
Sorbitan (20E.O.)
Methyl paraoxybenzoate 0.1
Nejirefama Bean Fruit Extract (Production Example) 1.0
Purified water balance ――――――――――――――――――――――

  As a result of the test, no side effects such as itching and dryness were observed. Further, this test sample was placed in a constant temperature bath at 40 ° C. and left for one month, but no precipitate was formed or discolored, and the stability was excellent.

  The present invention can provide cosmetics, food and drink, etc. as a composition for external use on skin and food. Examples thereof are given below, but the present invention is not limited to these examples.

Example 3 (skin lotion)
Ingredient name Blending amount A component (1) Olive oil 10.0
(2) Isopropyl myristate 1.0
(3) Monolauric acid 0.5
Polyoxyethylene (20) sorbitan (4) Propylene glycol 1.0
(5) Glycerin 2.0
B component (6) Nejirefamame fruit extract of production example 5.0
(7) Methyl paraben 0.1
(8) Ethanol 7.0
(9) Purified water remaining amount After each component A and component B were uniformly dissolved, the components A and component B were mixed, stirred and dispersed, and then filled into a container. In use, the contents are uniformly shaken and dispersed.

Example 4 (skin cream)
Ingredient name Compounding amount A component (1) dense wax 25.0
(2) Stearic acid 5.0
(3) Stearyl alcohol 5.0
(4) Reduced lanolin 2.0
(5) Squalene 2.0
(6) Monostearate sorbitan 3.0
Monostearate (7) Polyoxyethylenesorbitan 3.0
(8) Propylene glycol 5.0
B component (9) Nejirefamame fruit extract of production example 5.0
(10) Methylparaben 0.2
(11) Remaining amount of purified water After each component A and component B were heated and dissolved, the mixture was stirred and cooled to 30 ° C. to produce a skin cream.

Example 5 (tablets)
Ingredient name Blending amount (1) Nejirefamame fruit extract of production example 5.0
(2) Dextrin 63.0
(3) Sodium ascorbate 20.0
(4) Starch 5.5
(5) Silicon dioxide 3.0
(6) Glycerin fatty acid ester 1.5
(7) Fragrance 1.0
(8) Crystalline cellulose 1.0
Each of the above components was uniformly mixed, and a tablet was produced by a conventional method.

Example 6 (granule)
Ingredient name Blending amount (1) Nejirefamame fruit extract of production example 5.0
(2) Sodium ascorbate 60.0
(3) Dextrin 15.0
(4) Starch 10.0
(5) Lactose 9.0
(6) Crystalline cellulose 1.0
The above components were uniformly mixed and pulverized, and granules were produced by a conventional method.

Example 7 (jelly preparation)
Ingredient name Blending amount (1) Nejirefamame fruit extract of production example 5.0
(2) Dextrin 29.0
(3) Palatinose 5.0
(4) Gelatin 1.0
(5) Pectin 0.5
(6) Inositol 5.0
(7) Citric acid 0.8
(8) Ascorbic acid 3.0
(9) Nicotinamide 0.01
(10) Purified water remaining amount The above components were mixed and a jelly preparation was produced by a conventional method.

Example 8 (chewing gum)
Ingredient name Blending amount (1) Nejirefamame fruit extract of production example 1.0
(2) Gum base 29.0
(3) Maltitol 40.0
(4) Mannit 20.0
(5) Ascorbic acid 3.0
(6) Sorbitol 5.0
(7) Fragrance 1.0
(8) Purified water remaining amount The above components were mixed and chewing gum was produced by a conventional method.

According to the present invention, a safe, stable, and inexpensive skin external composition and food containing an extract of a torrefi bean fruit is provided, and rough skin caused by skin damage due to oxidation reaction, inflammation reaction, etc. caused by ultraviolet rays, etc. The composition for external use on the skin can be improved from outside the body, and the food can be improved from inside the body.

Claims (1)

A composition for external use for improving rough skin, comprising an extract obtained by extracting with an aqueous ethanol solution from a twisted bean fruit.