JP4482894B2 - Seaweed culture method - Google Patents

Description

The present invention relates to a method for culturing seaweed , and more particularly, to a method capable of realizing the purpose of artificial culture by a method for immobilizing floating germ cells released by wild large seaweed and mass-producing them.

Energy is indispensable in our daily life. For example, food and clothes, housing, transportation, education and entertainment are closely related to energy. With the prosperity of industry and commerce and the advanced development of material civilization, mankind faces enormous energy and environmental pressures.

Most of the existing energy is fossil fuels such as coal, oil, and natural gas, but the storage rate of fossil fuels is constant, and the incubation rate is very long, so the rate of fuel generation is slow, but the usage rate Is faster and further accelerates the loss of fossil energy. If a large amount of fossil fuel is used, acid rain is generated by generating warming gas easily, problems such as global warming are derived, and natural resources are depleted. Is not possible. According to the Energy Information Bureau of the US Department of Energy, each primary energy will be depleted in 40 years for oil, 60 years for natural gas, 200 years for coal, and uranium, which is atomic energy, for 70 years. Since general peasants are less aware of energy conservation, it is important to modify usage practices and raise awareness of energy conservation. On the other hand, active development of alternative energy and renewable energy is also an urgent issue.

In Taiwan, fossil energy is lacking and the development space for nuclear power is limited. Regenerative energy development and research are emphasized in order to provide enormous consumer and industrial energy. For example, research on renewable energy and alternative energy such as solar energy, geothermal energy, wind power, and tides has already been actively undertaken, but because of the lack of objective environmental conditions and high development costs, research on biomass energy And use is a proactive way of thinking. The Earth's biological resources are very abundant, and it is estimated that there are approximately 172.5 billion tons of biomaterials by photosynthesis annually on Earth. As a renewable energy resource, the energy of these biomaterials is 10 to 20 times the total energy consumption of the world, but at present, it has a very low utilization rate of only 1% to 3%. Therefore, it is best to develop fossil energy by developing energy conversion technology for the biomaterial energy. In addition, seaweed is one of the biomass energy materials, and it is necessary to maintain the production of seaweed when using biomass energy as a substitute for fossil energy. Currently, large algae are mostly wild, Its growth point is on the coast, sticking to coral, etc., its quality and production volume are limited by the weather and region, so there is a need for a method to artificially culture sea bream that can be mass-produced while being stable, Can provide high quality algae in large quantities and is advantageous for developing new energy.

The main object of the present invention is to artificially culture seaweed, which is stimulated by changes in osmotic pressure due to different salinity to release floating germ cells and to attach and stabilize germ cells However, the purpose of artificial culture that can be mass-produced is obtained, which is advantageous for algae production and can be provided in large quantities as a source of biomass energy material.

In order to achieve the above object, the present invention includes at least a, a step of preparing a seaweed culture solution, b, a step of sterilizing a culture dish having a seaweed attachment device, and then putting the seaweed culture solution in, C, collecting seaweed and placing it in a container with seawater; d, seaweed being stimulated by changes in osmotic pressure due to different salinity , releasing germ cells, and attaching the germ cells to seaweed Placing in a culture dish having a vessel, and placing the culture dish in a plant culture vessel to attach germ cells to the seaweed adhering device; e, when germ cells sprout into seaweed larvae, a step to put a conical flask having a seaweed cultivation liquid, is contained, a method of culturing algae.

FIG. 1 is a conceptual flow diagram of the present invention. As shown in the figure, the present invention is a method of culturing seaweed , and at least step a: sterilized seawater, seawater salinity is 3.5%, and 1 ml of saturated germanium oxide aqueous solution is added to 1 liter of seawater. Step b: preparing a seaweed culture solution, step b: sterilizing by a high-pressure steam sterilization method in a culture dish having a seaweed attachment device comprising a stainless steel frame and a cotton rope wound on the stainless steel frame; A step of introducing 180 ml to 220 ml of seaweed culture solution, step c: collecting seaweed and placing it in a container having seawater, and step d: seaweed being stimulated by changes in osmotic pressure due to different salinity Release the germ cells, put the germ cells into a culture dish with a seaweed adherence, and also put the culture dish into a plant incubator. In which the seaweed is subjected to different osmotic pressures and then left in a dark place for one day, and finally irradiated with light for 1 to 2 hours to produce germ cells, The seawater surface of the vessel is floated, germ cells are sucked up with a dropper and placed in a culture dish having the seaweed attachment device. The whole culture dish is placed in the plant culture device, and the temperature of the plant culture device is 20 A germ cell adheres to the seaweed attachment device under a culture condition of between 30 degrees and 30 degrees, illuminance between 145 and 155 μEm ⁻² s ^{−1 and} a photoperiod of 12/12 hours (light / dark); Step e: When germ cells sprout into the seaweed larvae, the seaweed larvae are put into a centrifuge tube containing 40 ml of seaweed culture, in which the germ cells adhere on the cotton rope of the seaweed attachment device, Sprouting on seaweed larvae with a length of 1 to 1.5 mm If that, the steps to put a Erlenmeyer flask seaweed broth 40ml removed cotton rope seaweed larvae attached of 5-7 centimeters in length is contained, it is contained.

According to the above method, when the seaweed is stimulated by the change in osmotic pressure due to different salinity , the floating germ cells are released, and the germ cells adhere, thereby stabilizing and mass-producing them. The purpose of artificial culture is realized.

Hereinafter, an example is given and demonstrated.

Example in which seaweed is Aosa The seaweed of this example is Aosa, and at least step a: sterilized seawater with a seawater salinity of 3.5% and 1 ml of saturated germanium oxide aqueous solution in 1 liter of seawater A step of preparing a seaweed culture solution to which is added, and step b: high pressure is applied to a culture dish having a seaweed adhering device comprising a 7 × 7 centimeter stainless steel frame and a 1 mm diameter cotton rope wound on the stainless steel frame. after sterilized by steam sterilization method, the steps interleaving seaweed culture about 200 ml, step c: Ulva was collected, and the collected were Ulva, washed with filtered seawater, also with or other seaweed The kimono is removed, washed with fresh water, and washed several times with clean seawater to simulate the “tidal” and “rainfall” environments in the intertidal zone ( osmotic pressure stimulation method ). Wrapping Aosa in wet paper towel containing seawater, letting it stand in a cool and dark place for 1 hour, leaving it in clean seawater overnight, and then putting it in a container having seawater; Step d: Aosa However, it is stimulated by changes in the osmotic pressure of different salinity to release germ cells, put the germ cells into a culture dish with a seaweed attachment, and put the culture dish into a plant incubator. The cells attach to the seaweed attachment device, and the Aosa is irradiated with light for 1 to 2 hours to generate germ cells. The germ cells float on the surface of the seawater of the container and suck the germ cells with a dropper. , Put into the culture dish having the seaweed attachment device, and put the whole culture dish into the plant culture device, the temperature of the plant culture device is 24 degrees, the illuminance is 150 μEm ⁻² s ⁻¹ , and the photoperiod 12/12 A step of attaching germ cells to the seaweed appendage under culture conditions that are between (light / dark), and step e: when germ cells sprout to Aosa larvae, the Aosa larvae are placed in an Erlenmeyer flask containing 40 ml of seaweed culture solution. When the germ cells adhere to the cotton rope of the seaweed attachment device and sprout to the Aosa larvae having a length of 1 to 1.5 mm, the Aosa larvae having a length of about 6 cm are attached. And removing the cotton rope and placing it in an Erlenmeyer flask containing 40 ml of seaweed culture solution.

As described above, the seaweed culturing method according to the present invention can effectively improve various conventional drawbacks, stimulate the seaweed with changes in osmotic pressure due to different salinity , and release floating germ cells, Then, the adherence of germ cells realizes the purpose of stabilization and mass production of artificial culture. Therefore, the present invention is more advanced and practical, so that a patent application is filed according to the law.

The above are merely preferred embodiments of the present invention, and the present invention is not limited thereby, and all equivalent changes and modifications made in accordance with the claims and the specification relating to the present invention relate to the present invention. Included within the scope of the claims.

Flow chart of the present invention

Step: ae

Claims (9)

at least,
a. preparing a seaweed culture solution;
b, sterilizing a culture dish having a seaweed attachment device, and then putting the seaweed culture solution;
c. collecting seaweed and placing it in a container with seawater;
d. Seaweeds are stimulated by changes in osmotic pressure due to different salinity to release germ cells, and the germ cells are placed in a culture dish having a seaweed attachment device. And attaching germ cells to the seaweed applicator,
e, when germ cells sprout into seaweed larvae, placing the seaweed larvae into an Erlenmeyer flask having a seaweed culture solution;
Contains,
A method for culturing seaweed characterized by the above. The seaweed cultivation method according to claim 1, wherein the seaweed is Aosa. The seaweed culture solution according to claim 1, wherein the seaweed culture solution is sterilized seawater and has a seawater salinity of 3.5%, and 1 ml of a saturated germanium oxide aqueous solution is added to 1 liter of seawater . Culture method . 2. The seaweed culturing method according to claim 1, wherein the seaweed adherence device comprises a stainless frame and a cotton rope wound on the stainless frame. In the said step b, the sterilization system is a high pressure steam sterilization method, The culture method of the seaweed of Claim 1 characterized by the above-mentioned. In the step d, seaweed, by light irradiation to generate a germ cell, seaweed culture methods according to claim 1 in which the germ cells, characterized in that the float seawater surface of the container. In step d, the culture conditions of the plant incubator are as follows: the temperature is 20 to 30 degrees, the illuminance is 145 to 155 μEm ⁻² s− ¹ , and the photoperiod is 12/12 hours (light / dark). The method for culturing seaweed according to claim 1, wherein: The volume of the said Erlenmeyer flask is 50 ml, and 40 ml of seaweed culture solutions are accommodated , The culture method of the seaweed of Claim 1 characterized by the above-mentioned. 8. The seaweed culture method according to claim 7, wherein the light irradiation time is 1 to 2 hours.