JP4520176B2 - Resin-degrading microbial culture - Google Patents

Description

  The present invention relates to a resin-degrading microbial culture. More specifically, the present invention relates to a resin, specifically a microbial culture that decomposes a polyester compound and / or a styrene acrylic compound, a resin, specifically a composition for decomposing a polyester compound and / or a styrene acrylic compound. And a method for recovering metals or pigments in waste.

Currently, incineration in an incinerator or the like is performed for the disposal of wastes such as resin-containing used toner generated when using a copier or printer. However, the treatment by incineration of resins may make it difficult to recover useful resources. Further, the treatment by incineration of resins has drawbacks such as the occurrence of breakage of an incinerator associated with the generation of high energy, the generation of CO _{2 and} the like.

  Therefore, biodegradable plastic resins such as polyhydroxyalkanoates have also been developed (Patent Document 1). However, such a biodegradable plastic resin still has the disadvantages that it is expensive and sometimes inferior in strength compared to a chemically synthesized resin (referred to as a synthetic resin).

  On the other hand, Pseudomonas bacteria (for example, patent documents) capable of decomposing waste containing polycaprolactone or poly (3-hydroxybutyric acid-3-hydroxyvaleric acid) copolymer as microorganisms that degrade synthetic resins. 2 and Patent Document 3), anaerobic bacteria Bacteroides genus bacteria (Patent Document 4) capable of degrading aliphatic polyesters, Mucor genus and Humicola capable of degrading aliphatic polyester resins. (Humicola), Thermomyces, Talaromyces, Chaetomium, Torula, Sporotrichum or Malbranke hea) filamentous fungus (Patent Document 5), and the like belonging to the genus.

However, in the microorganisms disclosed in Patent Documents 2 to 4, there is a drawback that the decomposition of the polyester resin may take several months after, for example, embedding a plastic sheet in the soil. In addition, the decomposition of the aliphatic polyester resin by the microorganism described in Patent Document 5 is assumed to be performed under high temperature conditions. In addition, degradation by these microorganisms may make it difficult to improve stability, and the types of resins that can be degraded may be limited.
WO99 / 42527 pamphlet JP-A-6-319532 JP-A-6-319533 JP-A-6-253865 JP-A-10-117768

One aspect of the present invention is to efficiently decompose a resin, specifically, a polyester compound and / or a styrene acrylic compound, to stably decompose a polyester compound and / or a styrene acrylic compound, a polyester compound, and / or Alternatively, there is substantially no generation of CO ₂ based on decomposition of the styrene acrylic compound, at least two microorganisms act cooperatively with each other, and the polyester compound and / or styrene acrylic are coordinated by at least two microorganisms. Decomposing compounds, facilitating recovery of metals or pigments in waste by decomposing polyester compounds and / or styrene acrylic compounds in waste, made with polyester compounds and / or styrene acrylic compounds Polyester after putting the material into the molded form By the compound and / or a styrene acrylic compound decomposes microorganisms, capable of achieving at least one of such to facilitate the recovery of the molded product of interest is to provide a microbial culture.

  Another aspect of the present invention is to provide a composition for degradation that can solve at least one of the same problems as those of the microorganism culture.

Another aspect of the present invention is to efficiently decompose a polyester compound and / or a styrene acrylic compound, to stably decompose a polyester compound and / or a styrene acrylic compound, various polyester compounds and / or styrene acrylic compounds. Decomposing, decomposing polyester compounds and / or styrene acrylic compounds at low cost, decomposing polyester compounds and / or styrene acrylic compounds with low impact on the surrounding environment, and decomposing polyester compounds and / or styrene acrylic compounds Substantially no generation of CO ₂ , facilitating recovery of metals or pigments in the waste by decomposing the polyester and / or styrene acrylic compounds in the waste, polyester compounds and / or Or styrene After the material is put into a mold made of a copper compound and molded, the polyester compound and / or the styrene acrylic compound can be decomposed by microorganisms to achieve at least one of making it easy to recover the desired molded product. Another object of the present invention is to provide a method for decomposing a polyester compound and / or a styrene acrylic compound.

  Still another aspect of the present invention is to facilitate recovery of a metal or a dye in the waste by decomposing a polyester compound and / or a styrene acrylic compound in the waste, a metal or a dye in the waste The metal in the waste, or at least one of recovering the polyester or compound and / or the styrene acrylic compound with a low influence on the surrounding environment. It is to provide a method for recovering a dye.

The present invention has been made in view of the above problems, that is, the gist of the present invention is as follows.
[1] A microorganism culture Do that contain at least two microorganisms exhibiting biodegradability of the polyester compound and / or a styrene acrylic compounds, at least two kinds of microorganisms
i) a microorganism consisting of Pseudomonas aeruginosa, Clavibacter xyli subsp. cynodontis and a microorganism consisting of Alkagenes sp. or a mixture of Alcaligenes sp.
ii) a microbial culture, which is a microbial mixture consisting of Pseudomonas aeruginosa and Sphingomonas paucimobilis,
[2] A composition for decomposing a polyester compound and / or a styrene acrylic compound, comprising the microorganism culture according to [1],
[3] A product or waste containing a polyester compound and / or a styrene acrylic compound is contacted with the microorganism culture according to [1] or the decomposition composition according to [2], Decomposition method of polyester compound and / or styrene acrylic compound, and [4] (A) waste containing polyester compound and / or styrene acrylic compound and metal or pigment, and (B) microbial culture according to [1] Or a method for recovering a metal or pigment in waste, which comprises contacting the composition for decomposition according to [2] above,
About.

The microorganism culture and decomposition composition of the present invention have an excellent effect of being able to efficiently and stably decompose a resin, specifically, a polyester compound and / or a styrene acrylic compound. Further, according to the microorganism culture and decomposition composition of the present invention, the generation of CO ₂ based on the decomposition of the polyester compound and / or the styrene acrylic compound is substantially suppressed, and the influence on the surrounding environment is low. There is an excellent effect that the polyester compound and / or the styrene acrylic compound can be decomposed. Furthermore, according to the microorganism culture and degradation composition of the present invention, at least two types of microorganisms act in a coordinated manner to become stable, and the polyester compound and / or the styrene acrylic compound are degraded in a coordinated manner. There is an excellent effect of being able to. Therefore, according to the microorganism culture and decomposition composition of the present invention, it is possible to facilitate recovery of metals or pigments in the waste by decomposing the polyester compound and / or the styrene acrylic compound in the waste. There is an excellent effect of being able to. In addition, according to the microorganism culture and decomposition composition of the present invention, the material is put into a mold made of a polyester compound and / or a styrene acrylic compound, and then the polyester compound and / or the styrene acrylic compound is decomposed by a microorganism. Thus, it is possible to easily collect the target molded product.

According to the method for decomposing a polyester compound and / or a styrene acrylic compound of the present invention, various polyester compounds and / or styrene acrylic compounds can be effectively and stably produced at low cost, and the generation of CO ₂ based on the decomposition is substantially reduced. It has an excellent effect of being able to be decomposed with a low influence on the surrounding environment. Moreover, according to the decomposition | disassembly method of this invention, there exists an outstanding effect that the collection | recovery of the metal or pigment | dye in the waste containing a polyester compound and / or a styrene acrylic compound can be performed easily. In addition, according to the decomposition method of the present invention, after molding the material into a mold made of a polyester compound and / or a styrene acrylic compound, the polyester compound and / or the styrene acrylic compound is decomposed by microorganisms to obtain the desired molding. There is an excellent effect that the collection of the object can be facilitated.

  According to the method for recovering metal or pigment in waste of the present invention, the metal or pigment in waste containing a polyester compound and / or a styrene acrylic compound can be easily and inexpensively recovered with a low impact on the surrounding environment. It has an excellent effect that it can be collected and can be easily collected.

  One aspect of the present invention is a microbial culture containing at least two microorganisms that exhibit the biodegradability of resins, specifically polyester compounds and / or styrene acrylic compounds.

  In the present specification, the “microorganism culture” refers to a group of microorganisms separated from nature exhibiting the biodegradability of a polyester compound and / or a styrene acrylic compound, or a mixed microorganism exhibiting a biodegradability equivalent to the microorganism group. It is a concept to include.

  The microorganism culture of the present invention has one major characteristic in that it contains at least two kinds of microorganisms exhibiting the biodegradability of polyester compounds and / or styrene acrylic compounds.

  In one aspect, the microorganism culture of the present invention is a group of microorganisms including at least two types of microorganisms found by the present inventors. According to such a group of microorganisms, the microorganism culture is used in toners for copying machines and printers. It exhibits an excellent property of enabling biodegradation of the hardly decomposable resin.

  Since the microorganism culture of the present invention contains at least two kinds of microorganisms exhibiting the biodegradability of a polyester compound and / or a styrene acrylic compound, at least at the time of cultivation for 5 weeks, compared to the case of using the microorganism alone. 1.1 times, specifically, 1.1 times to 1.5 times higher polyester compound and / or styrene acrylic compound biodegradability. Furthermore, in the microorganism culture of the present invention, at least two kinds of the microorganisms can act cooperatively, so that high biodegradability can be stably maintained with respect to the polyester compound and / or the styrene acrylic compound. Exhibits excellent effects. Moreover, in the microorganism culture of this invention, microorganisms are stably maintained. Therefore, according to the microorganism culture of the present invention, at least two kinds of microorganisms exhibiting the biodegradability of the polyester compound and / or the styrene acrylic compound act cooperatively, and the polyester compound and / or the styrene acrylic compound can be efficiently and stably stabilized. Can be broken down into

In addition, since the microorganism culture of the present invention contains the microorganism, the generation of CO ₂ based on the decomposition of the resin is substantially suppressed. Moreover, according to the microorganism culture of the present invention, since the microorganism contains at least two kinds, the polyester compound and / or the styrene acrylic compound can be decomposed cooperatively and used as nutrients in each growth. . Therefore, according to the microorganism culture of the present invention, the polyester compound and / or the styrene acrylic compound can be decomposed with a low influence on the surrounding environment.

  Examples of the polyester compound that can be decomposed by the microorganism culture of the present invention include alkyd resins, unsaturated polyester resins, thermoplastic polyester resins, and the like. Specifically, for example, phthalic anhydride, isophthalic acid, tetrachlorophthalic anhydride , Saturated polybasic acids such as hexachloroendomethylenetetrahydrophthalic anhydride, dimethylenetetrahydrophthalic anhydride, adipic acid and sebacic acid, unsaturated polybasic acids such as maleic anhydride and fumaric acid, ethylene glycol, propylene Polystyrene and polyhydric alcohols such as glycol, diethylene glycol, triethylene glycol, 1,3-butylene glycol, 2,3-butylene glycol, and 1,4-butylene glycol are polycondensed to produce unsaturated alkyd, and styrene, vinyl Toluene, diallylphthale Unsaturated polyester resin obtained by adding a polymerizable monomer such as methacrylic acid, crosslinking and curing; phthalic anhydride, isophthalic acid, tetrachlorophthalic anhydride, hexachloroendomethylenetetrahydrophthalic anhydride, Saturated polybasic acids such as dimethylenetetrahydrophthalic anhydride, adipic acid and sebacic acid, ethylene glycol, propylene glycol, diethylene glycol, triethylene glycol, 1,3-butylene glycol, 2,3-butylene glycol, 1,4 -Alkyd resins obtained by polycondensation with polyhydric alcohols such as butylene glycol; thermoplastic polyesters obtained by condensing compounds such as terephthalic acid and compounds such as ethylene glycol and butylene glycol.

  Specifically, examples of the polyester compound include maleic acid resin, polycarbonate (for example, polylactic acid), polyethylene terephthalate, polybutylene terephthalate, and the like.

Examples of the styrene acrylic compound that can be decomposed by the microorganism culture of the present invention include compounds obtained by polycondensation of an acrylic acid compound such as acrylic acid n-butyl ester and styrene.
Specifically, the styrene acrylic compound is not particularly limited, and examples thereof include acrylonitrile acrylate styrene and styrene butyl acrylate.

  In addition, the average molecular weight of the said polyester compound and / or a styrene acrylic compound, a polymerization degree, etc. should just be the range which exhibits the biodegradation by the microorganism culture of this invention.

  In another aspect, the microorganism culture of the present invention comprises at least two polyester compounds and / or styrene acrylic compounds belonging to a genus selected from the group consisting of Pseudomonas, Sphingomonas, Krabibacter, and Alkaligenes. One major feature is the inclusion of microorganisms that exhibit biodegradability. Preferably, the microorganism culture of the present invention comprises Pseudomonas aeruginosa (D-1 and R-1 in FIG. 5), Sphingomonas paucimobilis (D-2 in FIG. 5), Alkaline Genes. Alcaligenes sp. (R-3 in FIG. 5) and Clavibacter xyli subsp. Cynodontis (R-2 in FIG. 5) or bacteria that are close to the evolutionary tree At least two microorganisms selected from the group consisting of: In addition, these microorganisms are preserve | saved at the time of this application in the Kinki University Faculty of Agriculture Department of Agricultural Chemistry Department of Applied Microbiology (Postal Code: 631-8505 Nakamachi, Nara City, 3327-204). According to the “Regulations for Deposit of Microorganisms Related to Patent Applications” filed by the Patent Organism Depositary of the National Institute of Advanced Industrial Science and Technology (AIST).

The properties of Pseudomonas aeruginosa D-1 strain or bacteria close to it in the evolutionary tree are: short bacillus, gram-negative, motility: yes, colony characteristics: morphological properties of fluorescent substance production Have. Physiological properties of the D-1 strain or the bacteria close to it in the evolutionary tree are: growth pH range: 6.0 to 7.5, growth temperature range: 10 to 40 ° C., oxidase activity, β-galactosidase activity Mu, arginine dihydrolase activity Yes, lysine decarboxylase activity Yes, Mu ornithine decarboxylase activity, urease activity Mu, Mu tryptophan deaminase activity, gelatinase activity Mu, citric Sansi resistance Yes, Mu production of H ₂ S, the indole No production, Acetoin production Yes, Glucose assimilation Yes, D-mannitol assimilation No, Inositol assimilation No, D-sorbitol assimilation No, L-Rhamnose assimilation No, Saccharose assimilation No, D-melibiose assimilation Yes, D-amygdalin assimilation not available, L-arabinose assimilation not available.

The Sphingomonas paucimobilis strain D-2 or a bacterium that is close to the evolutionary tree has cocci, gram-negative, motility, and colony characteristics: white morphological properties. Physiological properties of the fungus in the position close to D-2 or its evolutionary tree are as follows: growth pH range: 6.0 to 7.8, growth temperature range: 20 to 40 ° C., no oxidase activity, β-galactosidase activity Yes, Arginine dihydrolase activity No, Lysine decarboxylase activity No, Ornithine decarboxylase activity No, urease activity No, tryptophan deaminase activity No, gelatinase activity No, citric acid assimilation No, H ₂ S production No, indole production No, production of acetoin Yes, glucose assimilation Yes, D-mannitol assimilation No, inositol assimilation No, D-sorbitol assimilation No, L-rhamnose assimilation No, saccharose assimilation No, D -No meribiose assimilation, D-amygdalin assimilation, no L-arabinose assimilation

The Pseudomonas aeruginosa R-1 strain or a bacterium that is close to the evolutionary tree is a short bacillus, gram-negative, motility, colony characteristics: morphological properties of fluorescent substance production. Physiological properties of the R-1 strain or a fungus close to it in the evolutionary tree are: growth pH range: 6.0 to 7.5, growth temperature range: 10 to 40 ° C., oxidase activity, β-galactosidase activity Mu, arginine dihydrolase activity Yes, Mu lysine decarboxylase activity, Mu ornithine decarboxylase activity, urease activity Mu, Mu tryptophan deaminase activity, gelatinase activity Mu, citric Sansi resistance Yes, H ₂ Mu S production, the indole Production None, Acetoin Production None, Glucose Utilization Available, D-Mannitol Utilization None, Inositol Utilization None, D-Sorbitol Utilization None, L-Rhamnose Utilization None, Saccharose Utilization None, No D-melibiose assimilation, D-amygdalin assimilation, L-arabinose assimilation.

Clavibacter xyli subsp. Cynodontis R-2 strain or bacteria close to it in the evolutionary tree are short bacillus, gram positive, motility, colony characteristics: central brown morphological Has properties. Physiological properties of the R-2 strain or bacteria close to the evolutionary tree are as follows: growth pH range: 6.0-7.5, growth temperature range: 20-37 ° C., no oxidase activity, β-galactosidase activity Yes, arginine dihydrolase activity Yes, Mu lysine decarboxylase activity, Mu ornithine decarboxylase activity, urease activity Mu, Mu tryptophan deaminase activity, gelatinase activity Mu, Mu citric Sansi resistance, Mu production of H ₂ S, the indole Production None, Production of acetoin Yes, Glucose assimilation None, D-mannitol assimilation None, Inositol assimilation None, D-sorbitol assimilation None, L-Rhamnose assimilation None, Sucrose utilization No D-melibiose assimilation, D-amygdalin assimilation, L-arabinose assimilation.

Alkaline genes sp. Alkalinenes sp. R-3 or bacteria in a position close to the evolutionary tree have cocci, gram negative, motility, colony characteristics: white morphological properties. Physiological properties of the R-3 strain or bacteria close to the evolutionary tree are as follows: growth pH range: 6.0 to 7.5, growth temperature range: 20 to 40 ° C., no oxidase activity, β-galactosidase activity Mu, arginine dihydrolase activity Yes, Mu lysine decarboxylase activity, Mu ornithine decarboxylase activity, urease activity Yes, tryptophan deaminase activity Yes, gelatinase activity Mu, citric Sansi resistance Yes, H ₂ Mu S production, the indole Production None, Production of acetoin Yes, Glucose assimilation None, D-mannitol assimilation None, Inositol assimilation None, D-sorbitol assimilation None, L-Rhamnose assimilation None, Sucrose utilization No D-melibiose assimilation, D-amygdalin assimilation, L-arabinose assimilation.

  Specifically, as the microorganism culture of the present invention, the D-1 strain, the D-2 strain, the R-1 strain, the R-2 strain, and the R-3 strain or the bacteria that are close to the evolved phylogenetic tree. A mixed microorganism containing at least two species selected from the group consisting of, specifically, a mixed microorganism named and displayed as AMOC001 (this mixed microorganism is deposited by the Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology) A microorganism group comprising the R-1 strain, the R-2 strain, and the R-3 strain; the D-1 strain, the D-2 strain, the R-1 strain, the R-2 strain, and the R -3 strains of microorganisms and the like. From the viewpoint of sufficiently exhibiting high biodegradability stably, preferably, the mixed microorganism AMOC001, the microorganism group containing the R-1 strain, the R-2 strain, and the R-3 strain, the D-1 strain, A microorganism group containing the D-2 strain, the R-1 strain, the R-2 strain, and the R-3 strain is desirable.

  According to the microorganism culture of the present invention, for example, at least about 1.1 times, compared to the case where each of the D-1, D-2, R-1, R-2 and R-3 is used alone, Specifically, it exhibits an excellent effect of developing a polyester compound biodegradability of about 1.1 to 1.5 times.

The biodegradability of each of the polyester compound and the styrene acrylic compound by the microorganism culture of the present invention is, for example,
-E medium containing a polyester compound with a final concentration of 10 g / l or a styrene acrylic compound with a final concentration of 15 g / l [Composition: NH ₄ Cl 0.25 wt%, KH ₂ PO ₄ 0.1 wt%, K ₂ HPO ₄₀ 0.05% by weight, MgSO ₄ .7H ₂ O 0.05% by weight, NaCl 0.2% by weight, ZnCl ₂ 0.001% by weight, FeSO ₄ .7H ₂ O 0.001% by weight, CaCl 2 0.001% by weight PH 7.0] Incubate the microbial culture in a test tube containing 5 ml at 37 ° C. for 1 week at 125 strokes / minute,
-Stearyl alcohol was added as an internal standard to 5 ml of the obtained culture solution so that the final concentration was 3.7 mM.-5 ml of dichloromethane was added to 6 ml of the obtained mixture, and the polyester compound or styrene acrylic in the culture solution was added. Extracting a compound and a degradation product derived from the polyester compound or a degradation product derived from a styrene acrylic compound,
-Obtained the culture broth-dichloromethane two-phase supernatant, dried,
-1 μl of the sample obtained by adding 5 ml of dichloromethane to the resulting product is spotted on a TLC rod, eg trade name: Chromarod-SIII (100 mm × 0.5 mm),
-The obtained TLC rod was developed for 7 cm at 40 ° C in a developing tank containing a developing solution of n-hexane: toluene = 2: 8 (v / v) until the internal standard was visible on the rod after drying. Perform FID analysis,
-The rod is developed for 10 cm in a developing tank containing a dichloromethane: methanol / 95: 5 developing solution, and the dried rod is subjected to FID analysis.
It can be evaluated by calculating the decomposition rate when the peak area of the resin component is zero. As analysis conditions in the FID analysis, for example, when using a product name: Iatroscan Mk-6s manufactured by Yatron, hydrogen gas 160 / ml, air 2.0 l / min, scan speed 30 seconds / Scan conditions are listed.

  The microbial culture of the present invention can be selected from, for example, soil (for example, soil at the Kinki University Department of Agriculture) or compost by the biodegradation evaluation method described above.

  The microorganism culture of the present invention may further contain other microorganisms that can capture or assimilate the products produced by the degradation of the polyester compound and / or the styrene acrylic compound, if necessary.

The microorganism culture of the present invention is, for example, 0.2 to 2.0% by weight, preferably 0.75 to 1.25% by weight, NH ₄ Cl, (NH ₄ ) ₂ SO ₄ , NH ₄ as a nitrogen source. It contains at least one of NO ₃ , (NH ₄ ) ₂ CO, (NH ₄ ) ₂ HPO ₄ , (NH ₄ ) H ₂ PO ₄ , and the carbon source / nitrogen source amount ratio (C / N ratio) is 4 to 100, preferably 6 to 75, particularly preferably 12 to 50, pH 5.0 to 8.0, preferably 6.5 to 7.5, for example, E medium, F medium [Composition: NH ₄ Cl 1 wt%, KH ₂ PO ₄ 0.1 wt%, K ₂ HPO ₄ 0.05 wt%, MgSO ₄ .7H ₂ O 0.05 wt%, NaCl 0.2 wt%, ZnCl ₂ 0.001 _{wt%, FeSO 4 · 7H 2 O} 0.001 wt%, CaCl ₂ 0.001 wt%, pH 7.0 Or the like by, 25 to 45 ° C., preferably, under conditions of 33 to 40 ° C., if necessary, 90 to 140 strokes / min, preferably may be maintained by 110-130 strokes / min incubation. In addition, the said culture medium is arbitrarily made into a final concentration of 0.01 to 0.5% by weight, preferably 0.1% from the viewpoint of more stably and sufficiently exhibiting the biodegradability of the polyester compound and / or the styrene acrylic compound. Weight% Yeast extract may be contained.

  According to the microorganism culture of the present invention, the resin in the waste, specifically, the polyester compound and / or the styrene acrylic compound can be decomposed. Therefore, according to the microorganism culture of the present invention, it is possible to facilitate the recovery of the metal or pigment contained in the waste.

  In addition, according to the microorganism culture of the present invention, the material can be easily recovered by decomposing the resin with microorganisms after putting the material into a mold made of resin and molding.

  Another aspect of the present invention is a composition for decomposing a polyester compound and / or a styrene acrylic compound.

  The degradation composition of the present invention has one major feature in that it contains the microorganism culture of the present invention. Therefore, according to the composition for decomposition of the present invention, various polyester compounds and / or styrene acrylic compounds can be decomposed efficiently, stably and inexpensively with a low influence on the surrounding environment.

In addition, since the decomposition composition of the present invention contains the microbial culture of the present invention, when used for the decomposition of a polyester compound and / or a styrene acrylic compound, the generation of CO ₂ due to the decomposition is substantially reduced. Is suppressed. In addition, according to the decomposition composition of the present invention, since the microorganism culture of the present invention is contained, when the polyester compound and / or styrene acrylic compound is used for decomposition, the polyester compound and / or styrene acrylic compound is used. Can be decomposed and used as a nutrient source for growth. Therefore, according to the decomposition composition of the present invention, the polyester compound and / or the styrene acrylic compound can be decomposed with a low influence on the surrounding environment.

  The decomposing composition of the present invention may contain various reagents and the like that stably maintain and survive microorganisms exhibiting the biodegradability of the polyester compound and / or styrene acrylic compound.

  Examples of the reagent that stably maintains the microorganism and allows it to survive include a medium suitable for the growth and culture of the microorganism, a carrier that can immobilize or hold the microorganism, and the like.

The nitrogen source in the medium is 0.2 to 2.0% by weight, preferably 0.75 to 1.25% by weight NH ₄ Cl, (NH ₄ ) ₂ SO ₄ , NH ₄ NO ₃ , (NH ₄ ) ₂ CO, (NH ₄ ) ₂ HPO ₄ , (NH ₄ ) H ₂ PO _4, etc., and the carbon source / nitrogen source amount ratio (C / N ratio) is 5 to 100, preferably 10 to 70 Especially preferably, it is desirable that it is 12-50.

Specifically, for example, the E medium, F medium [composition: NH ₄ Cl 1 wt%, KH ₂ PO ₄ 0.1 wt%, K ₂ HPO ₄ 0.05 wt%, MgSO ₄ .7H ₂ O 0 0.05 wt%, NaCl 0.2 wt%, ZnCl ₂ 0.001 wt%, FeSO ₄ .7H ₂ O 0.001 wt%, CaCl ₂ 0.001 wt%, pH 7.0] and the like. In addition, the said culture medium is arbitrarily made into a final concentration of 0.01 to 0.5% by weight, preferably 0.1% from the viewpoint of more stably and sufficiently exhibiting the biodegradability of the polyester compound and / or the styrene acrylic compound. Weight% Yeast extract may be contained.

  The carrier that can immobilize or hold the microorganism may be any carrier that can stably maintain the growth of microorganisms exhibiting the biodegradability of the polyester compound and / or styrene acrylic compound and does not inhibit the biodegradation. Examples thereof include a porous carrier, a nonwoven fabric, activated carbon and the like. In the composition for decomposition of the present invention, the microorganism culture of the present invention may be immobilized on the surface of the insoluble carrier, or may be immobilized or held inside. When the microorganism culture of the present invention is immobilized or held inside such a carrier, it is a carrier capable of giving and receiving various nutrients necessary for the growth of the microorganism, a polyester compound to be decomposed, and / or a styrene acrylic compound, etc. It is desirable.

  The number of microbial cells in the decomposing composition of the present invention is not particularly limited, and may be appropriately set according to, for example, the type of carrier, handling method, and the like.

  The decomposition composition of the present invention is a compound, reagent, polyester compound and / or product produced by decomposition of a styrene acrylic compound capable of capturing a product produced by decomposition of a polyester compound and / or a styrene acrylic compound, if necessary. It may further contain other microorganisms that can capture or assimilate.

  According to the decomposition composition of the present invention, the polyester compound and / or the styrene acrylic compound in the waste can be decomposed. Therefore, the decomposition composition of the present invention can also be used for facilitating recovery of metals or pigments contained in waste.

  According to the composition for decomposition of the present invention, after putting a material into a mold made of resin and molding, the polyester compound and / or the styrene acrylic compound is decomposed by microorganisms to facilitate recovery of the target molded product. Can also be used.

  Another aspect of the present invention is a polyester characterized by contacting a product or waste containing a polyester compound and / or a styrene acrylic compound with the microbial culture of the present invention or the decomposition composition of the present invention. This is a method for decomposing a compound and / or a styrene acrylic compound.

  The degradation method of the present invention has one major feature in that the microorganism culture of the present invention or the degradation composition of the present invention is used. Therefore, according to the decomposition method of the present invention, various polyester compounds and / or styrene acrylic compounds can be decomposed efficiently, stably and inexpensively.

In addition, the degradation method of the present invention has one major feature in that the product or waste containing the polyester compound and / or the styrene acrylic compound is brought into contact with the microbial culture of the present invention or the degradation composition of the present invention. There is. Therefore, according to the decomposition method of the present invention, the generation of CO ₂ due to decomposition is substantially suppressed, and the product produced when the polyester compound and / or the styrene acrylic compound is decomposed is the microbial culture of the present invention. Or since it can be used as a nutrient in the growth of microorganisms contained in the composition for decomposition of the present invention, the polyester compound and / or the styrene acrylic compound can be decomposed with a low influence on the surrounding environment.

  Examples of the product include a mold for molding.

  Examples of the waste include toners, can laminates, insulators for electronic devices, home appliance / car waste, industrial waste, and the like.

In the decomposition method of the present invention, the contact between the product or waste containing the polyester compound and / or the styrene acrylic compound and the microorganism culture of the present invention or the decomposition composition of the present invention is, for example,
1) culturing the microorganism culture of the present invention in the presence of the product or waste,
2) The composition for decomposition of the present invention may be sprayed or mixed and maintained on the product or waste under conditions suitable for the growth of microorganisms exhibiting the biodegradability of polyester compounds and / or styrene acrylic compounds. Can be mentioned.

  If necessary, the waste may be pulverized by an appropriate means into a powder form.

  The “conditions suitable for the growth of microorganisms exhibiting the biodegradability of the polyester compound and / or styrene acrylic compound” may be the same as the conditions applied to the maintenance of the microorganism culture of the present invention.

  Further, according to the decomposition method of the present invention, when used in molding processing, the material is put into a mold made of a product (resin) containing a polyester compound and / or a styrene acrylic compound and molded. In this case, the product (resin) and the microorganism culture of the present invention or the decomposition composition of the present invention are brought into contact with each other to decompose the polyester compound and / or the styrene acrylic compound in the product, thereby obtaining the desired molded product. Can be easily recovered.

  According to the decomposition method of the present invention, the polyester compound and / or the styrene acrylic compound in the waste containing the polyester compound and / or the styrene acrylic compound is efficiently decomposed. The component becomes a residue. Therefore, the decomposition method of the present invention can facilitate recovery of metals, pigments and the like in the waste.

  Still another aspect of the present invention provides (A) a waste containing a polyester compound and / or a styrene acrylic compound and a metal or a pigment, and (B) a microorganism culture of the present invention or a decomposition composition of the present invention. It is a method for recovering metals or pigments in waste, which is characterized by contacting them.

  The recovery method of the present invention has one major feature in that (B) the microorganism culture of the present invention or the decomposition composition of the present invention is used. Therefore, according to the recovery method of the present invention, the metal or the pigment in the waste containing the polyester compound and / or the styrene acrylic compound can be easily and inexpensively recovered.

Further, the recovery method of the present invention comprises (A) a waste containing a polyester compound and / or a styrene acrylic compound and a metal or a pigment, and (B) the microorganism culture of the present invention or the decomposition composition of the present invention. There is one major feature in contacting. Therefore, according to the recovery method of the present invention, the generation of CO ₂ based on the decomposition of the polyester compound and / or the styrene acrylic compound in the waste is substantially suppressed, and the polyester compound and / or the styrene acrylic compound is decomposed. The products produced in the process can be used as nutrients in the growth of microorganisms contained in the microorganism culture of the present invention or the decomposition composition of the present invention, so that the metal or pigment in the waste has a low impact on the surrounding environment. Can be recovered.

  The contact between (A) a waste containing a polyester compound and / or a styrene acrylic compound and a metal or a pigment, and (B) the microorganism culture of the present invention or the decomposition composition of the present invention is the same as the decomposition method of the present invention. It is the same.

  In the product after differentiation of the polyester compound and / or styrene acrylic compound, the components composed of the polyester compound and / or styrene acrylic compound are substantially removed. It can be easily recovered by fractionation, acid-alkali treatment, precipitation, organic solvent extraction, centrifugation, various chromatography, and the like.

  EXAMPLES Hereinafter, although an Example demonstrates this invention, this invention is not limited at all by this Example.

  Soil, compost, sludge, seawater, etc. were sampled from nature. The solid sample was suspended in physiological saline and uniformly dispersed by ultrasonic treatment, and the obtained sample was used for the following operations. Moreover, liquid samples, such as seawater, were used for the following operations as they were.

  The sampled samples were screened as follows using the activity of decomposing each of the polyester compound and the styrene acrylic compound, which are fine powder resins used in the toner ink, as indices.

Fine powder polyester resin for toner ink (supplied by Mikasa Sangyo Co., Ltd.) and fine powder styrene acrylic resin for toner ink (supplied by Mikasa Sangyo Co., Ltd.) were dissolved in dichloromethane, dispensed into test tubes, and air-dried. Thereafter, the E medium [composition: NH ₄ Cl 0.25 wt%, KH ₂ PO ₄ 0.1 so that the final concentration of the polyester compound is 10 g / l and the final concentration of the styrene acrylic compound is 15 g / l. Wt%, K ₂ HPO ₄ 0.05 wt%, MgSO ₄ .7H ₂ O 0.05 wt%, NaCl 0.2 wt%, ZnCl ₂ 0.001 wt%, FeSO ₄ · 7H ₂ O 0.001 wt% %, CaCl ₂ 0.001 wt%, pH 7.0] was added to the test tube. Thereafter, the test tube containing the medium was autoclaved at 121 ° C. for 20 minutes.

  Product name: Hydroball (Yonkid, Netherlands) 0.2 g was added to the medium, and then 500 μl of the sample was added, and the culture was concentrated at 37 ° C. for 1 week at 125 strokes / minute. It was.

  After the culture, the polyester compound or styrene acrylic compound adhering to the test tube wall is dispersed in the medium, and the E medium containing the polyester compound or the E medium containing the styrene acrylic compound becomes cloudy in the sample. As an indicator of the presence of a microorganism group that degrades the polyester compound or a microorganism group that degrades the styrene acrylic compound, an enriched culture solution with good growth of microorganisms was selected.

  Thereafter, a part of the enriched culture broth was used as an E medium and / or styrene containing the polyester compound under the condition of 125 g / min at 37 ° C. in the presence of 0.2 g of a trade name: Hydroball (manufactured by Yonkid). Culture was performed for 1 week using 5 ml of E medium containing an acrylic compound. The result of observing the obtained culture solution is shown in FIG.

  As a result, as shown in the panel (A) and panel (B) of FIG. 1, a microorganism group capable of degrading a polyester compound and two microorganism groups capable of degrading a styrene acrylic compound [microorganism group A (derived from compost) And microorganism group F (AMOC001) (derived from the soil on the campus of the Faculty of Agriculture, Kinki University)].

  For each microorganism group A and microorganism group F obtained in Example 1, the influence of the number of passages on the degradation rate of the polyester compound or styrene acrylic compound was examined.

  As in Example 1, trade name: Hydroball [manufactured by Yonkid] 0.2 g and final concentration 10 g / l polyester resin [supplied by Mikasa Sangyo Co., Ltd.] or final concentration 15 g / l styrene acrylic resin [Mikasa Sangyo Co., Ltd.] The microorganism group A was cultured under conditions of 125 strokes / minute at 37 ° C. in 5 ml of E medium containing

  Product name: Hydroball (manufactured by Yonkid) 0.2 g and final concentration 10 g / l Polyester resin [supplied by Mikasa Sangyo Co., Ltd.] E medium 5 ml Four (5 ml / tube) were cultured.

  Subculture was performed every week, and 4 to 3 culture solutions were sampled as quantitative samples each time subculture was performed.

  As an internal standard, stearyl alcohol (manufactured by Nacalai Tesque) was added to 5 ml of the obtained culture broth to a final concentration of 3.7 mM. To 6 ml of the obtained mixture, 5 ml of dichloromethane was added to extract a polyester compound or a styrene acrylic compound, a degradation product derived from the polyester compound, a degradation product derived from the styrene acrylic compound, and the like in the culture solution. The obtained culture broth-dichloromethane two-phase system was centrifuged at 2385 × g for 10 minutes to obtain a supernatant. The extraction with dichloromethane was repeated once more, and the extracts were combined. Thereafter, the obtained extract was dried using an evaporator.

  To the resulting product was added 5 ml of dichloromethane. The obtained sample was analyzed by a thin layer chromatography / hydrogen flame ionization detector method (hereinafter referred to as TLC / FID) using a product name: Iatroscan Mk-6s manufactured by Yatron Co., Ltd. as follows. The analysis conditions were hydrogen gas 160 / ml, air 2.0 l / min, and scan speed 30 seconds / scan.

1 μl of the sample was spotted on Chromarod-SIII (100 mm × 0.5 mm) and left in a constant humidity bath for 10 minutes. Thereafter, the rod was placed in a developing tank containing a developing solution of n-hexane: toluene = 2: 8 (v / v) and developed at 40 ° C. for 7 cm. Then, after removing the solvent, the rod was dried. After performing the FID analysis until the internal standard was seen, the rod was left in a constant humidity bath for 10 minutes. Thereafter, the rod was placed in a developing tank containing a developing solution of dichloromethane: methanol / 95: 5 and developed for 10 cm. After development, the rod was dried at room temperature and subjected to FID analysis. The results are shown in FIG. In FIG. 2, the decomposition rate is 100% when the peak area of the resin component is zero.

  As a result, as shown in FIG. 2, the microorganism group A (circle in the figure) and the microorganism group F (triangle in the figure) are substantially affected by subculture when subcultured for 12 generations or more. It can be seen that the polyester is stably decomposed at a decomposition rate of about 70 to 80%.

  On the other hand, as shown in FIG. 2, it can be seen that the microorganism group A (indicated by squares in the figure) decomposes styrene acrylic until the third subculture.

  For microorganism group A, the culture conditions suitable for the degradation of styrene acrylic were examined.

Fine powder styrene acrylic resin for toner ink (supplied by Mikasa Sangyo Co., Ltd.) was dissolved in dichloromethane, dispensed into a test tube, and air-dried. Thereafter, F medium [composition: NH ₄ Cl 1 wt%, KH ₂ PO ₄ 0.1 wt%, K ₂ HPO ₄ 0.05 wt%, so that the final concentration of styrene acrylic compound is 15 g / l, MgSO ₄ .7H ₂ O 0.05 wt%, NaCl 0.2 wt%, ZnCl ₂ 0.001 wt%, FeSO ₄ .7H ₂ O 0.001 wt%, CaCl ₂ 0.001 wt%, pH 7.0 ] 5 ml was added to the test tube. Thereafter, the test tube containing the medium was autoclaved at 121 ° C. for 20 minutes. The F medium is a medium in which the concentration of NH ₄ Cl is increased four times compared to the E medium.

  Product name: Hydroball (manufactured by Yonkid) In the presence of 0.2 g, four microorganism groups A are cultured in the F medium containing the styrene acrylic compound at 37 ° C. and 125 strokes / minute, every week. Three cultures were sampled.

  As an internal standard, stearyl alcohol (manufactured by Nacalai Tesque) was added to 5 ml of the obtained culture broth to a final concentration of 3.7 mM. To 6 ml of the obtained mixture, 5 ml of dichloromethane was added to extract a styrene acrylic compound or a decomposition product derived from the styrene acrylic compound in the culture solution. The obtained culture broth-dichloromethane two-phase system was centrifuged at 2385 × g for 10 minutes to obtain a supernatant. The extraction with dichloromethane was repeated once more, and the extracts were combined. Thereafter, the obtained extract was dried using an evaporator.

  To the resulting product was added 5 ml of dichloromethane. The obtained product was analyzed by TLC / FID in the same manner as in Example 2. The results are shown in panel (A) of FIG. In the panel (A) of FIG. 3, the decomposition rate is shown as 100% when the peak area of the resin component is zero.

As a result, as shown in the panel (A) of FIG. 3, it is understood that the decomposition rate can be maintained even if cultured for a long period of time by increasing the concentration of NH ₄ Cl in the medium to 4 times that in the E medium. .

  Next, 4 microorganism groups A were cultured under the condition of 125 strokes / minute at 37 ° C. using 5 ml of the same medium as described above, and subcultured every week. Three culture solutions were sampled for each subculture, and the decomposition rate of the styrene acrylic compound was analyzed by TLC / FID in the same manner as described above. The results are shown in panel (B) of FIG. In the panel (B) of FIG. 3, the decomposition rate is shown as 100% when the peak area of the resin component is zero.

As a result, as shown in the panel (B) of FIG. 3, by increasing the concentration of NH ₄ Cl in the medium to 4 times that in the E medium, the resolution can be maintained even when subculture is performed. Recognize.

  Further, yeast extract (manufactured by Difco Laboratories) was added to the F medium containing styrene acrylic so as to have a final concentration of 0.1% by weight, and subculture was performed in the same manner as described above. The decomposition rate of the styrene acrylic compound was analyzed by TLC / FID. The results are shown in panel (A) of FIG. In the panel (A) of FIG. 4, the decomposition rate is shown as 100% when the peak area of the resin component is zero.

  As a result, as shown in panel (A) of FIG. 4, it can be seen that by using a medium containing yeast extract, an average 30% degradation rate can be maintained even when subculture is performed.

  Furthermore, the yeast extract concentration was set to 0.1, 0.25, or 0.5% by weight, and the microorganism group A was cultured at 37 ° C. for 125 strokes / minute for 1 week. The results are shown in panel (B) of FIG. In the panel (B) of FIG. 4, the decomposition rate is shown as 100% when the peak area of the resin component is zero.

  As a result, as shown in the panel (B) of FIG. 4, it can be seen that styrene acrylic can be decomposed at a decomposition rate of about 70% by using a medium containing 0.1 wt% yeast extract.

  Microbial groups A and F were serially diluted with sterile saline. Each of the obtained dilutions was applied to a trade name: R2A plate medium (manufactured by Difco Laboratories) and cultured at 37 ° C. until colonies appeared. Colonies with different morphologies were selected from the emerged colonies and applied again to the R2A medium. As a result, single colonies of three types of microorganisms (R-1, R-2, R-3) and two types of microorganisms (D-1, D-2) were isolated from the microorganism group A. It was done. About each isolated microorganism, the form and the mobility were investigated with the phase-contrast microscope (made by Nikon). The morphological characteristics of each isolated microorganism are shown in FIG.

  Each isolated microorganism was then subjected to Gram staining and oxidase test by conventional methods, and other physiological and biochemical properties were examined using a product name: Api20E identification kit manufactured by BioMerieux. The results are shown in Table 1.

For each isolated microorganism, DNA was extracted by a conventional method, 16S rDNA was amplified by PCR using the obtained DNA as a template, and the base sequence of the 16S rDNA was analyzed. In amplification by the PCR method, 10 × Gold Taq buffer (Applied Biosystems) 5 μl, dNTP mixture (Applied Biosystems, 2.0 mM each) 5 μl, 25 mM MgCl ₂ 6 μl, PCR primer pair [Toa Gosei Co., Ltd. Supply, 10 pmol / μl each] 1 μl each, sample and ultrapure water 31.5 μl, AmpliTaq Gold [Applied Biosystems 5U / μl] reaction system (50 μl) was used. The PCR primer pair is a forward primer having the base sequence shown in SEQ ID NO: 1 and a reverse primer having the base sequence shown in SEQ ID NO: 2.

  PCR conditions are as follows: after a pre-run at 94 ° C. for 7 minutes, a reaction of 20 cycles of 94 ° C. for 1 minute, 65 ° C. for 1 minute (down 0.5 ° C. every cycle) and 72 ° C. for 2 minutes is performed Then, the reaction was carried out for 15 cycles of 94 ° C. for 1 minute, 55 ° C. for 1 minute, and 72 ° C. for 2 minutes, and post-run at 72 ° C. for 10 minutes.

  Each obtained amplification product was purified by 1 wt% low melting point agarose gel (manufactured by Bioproducts, trade name: SeaPlaque GTG agarose) to obtain a sample for base sequence analysis. Using the obtained sample for base sequence analysis, 8 primers of the base sequences shown in SEQ ID NOs: 3 to 10, and a product name: DYEamic ET Terminator Cycle Sequencing Kit (manufactured by Amersham Biosciences) Reaction was performed. Thereafter, the obtained product was analyzed with a DNA sequencer [manufactured by Applied Biosystems, trade name: ABI PRISM 3100 DNA sequencer]. Using the database registered in DNA Data Bank of Japan (DDBJ), a homology search was performed on the base sequence of the 16S rDNA that was found.

  From the results shown in FIG. 5, the results shown in Table 1, and the results of 16sRNA analysis, D-1 is Pseudomonas aeruginosa, D-2 is Sphingomonas paucimobils, and R-1 is Pseudomonas. Elginosa (Pseudomonas aeruginosa), R-2 is Clavibacter xyli subsp. Cynodontis, R-3 is Alkaline Genes sp.

  For the isolated microorganisms R-1, R-2, R-3, D-1, and D-2, the polyester degradation rate was examined.

  Each isolated microorganism was treated with 125 strokes / minute at 37 ° C. in 5 ml of F medium containing 0.2 g of a product name: Hydroball (manufactured by Yonkid) and a final concentration of 10 g / l polyester resin (supplied by Mikasa Sangyo Co., Ltd.). The culture was performed under conditions, and the degradation rate of the polyester compound was analyzed by the TLC / FID method in the same manner as in Example 2. The results are shown in panel (A) of FIG. In the figure, the decomposition rate is shown as 100% when the peak area of the resin is zero.

  In addition, the isolated microorganisms R-1, R-2, and R-3 were mixed at a ratio of 1: 1: 1, respectively, to a suspension of microorganisms adjusted to the turbidity of McFarland 4, and mixed with microorganisms. The product was obtained. Further, D-1 and D-2 were mixed at a ratio of 1: 1, respectively, to a microbial suspension in which the turbidity of McFarland 4 was adjusted to obtain a microbial mixed product D. Moreover, R-1, R-2, R-3, D-1, and D-2 were mixed to obtain a microorganism mixed product RD.

  In 5 ml of F medium containing 0.2 g of the product name: Hydroball (manufactured by Yonkid) and a final concentration of 10 g / l polyester resin (supplied by Mikasa Sangyo Co., Ltd.) Four cultures were performed at 37 ° C. under the condition of 125 strokes / minute. Three culture solutions were sampled at the first week and the fifth week from the start of the culture, and the degradation rate of the polyester compound was analyzed by the TLC / FID method in the same manner as in Example 2. The results are shown in panel (B) of FIG. In the figure, the decomposition rate is shown as 100% when the peak area of the resin is zero.

  As a result, as shown in FIG. 6, by culturing the isolated microorganisms in combination, the degradation rate of the polyester compound is increased to the same or more than when each isolated microorganism is cultured alone. I understand that. Moreover, it turns out that the degradation rate by the microorganism mixed product R which mixed R-1, R-2, and R-3 and the degradation rate by the microorganism group A which is a separation source are equivalent. Furthermore, it turns out that the decomposition rate by the microorganism mixed product D which mixed D-1 and D-2 and the decomposition rate by the microorganism group F which is a separation source are equivalent. Moreover, the degradation rate by the microorganism mixed product RD obtained by mixing R-1, R-2, R-3, D-1, and D-2 is equal to the degradation rate by the microorganism group A and the microorganism group F, respectively. I understand.

  Using microorganism group A, degradation of polyethylene terephthalate was examined.

  Polyethylene terephthalate was chopped, put into a test tube, and dissolved with 1,1,1,3,3,3-hexafluoro-2-propanol (manufactured by Nacalai Tesque). After air drying, 5 ml of F medium was added to the test tube. Thereafter, the test tube containing the medium was autoclaved at 121 ° C. for 20 minutes.

  Product name: Hydroball (manufactured by Yonkid) In the presence of 0.2 g, the microorganism group A was cultured for 1 week at 37 ° C. under the condition of 125 strokes / minute in F medium containing polyethylene terephthalate. The result of observing the obtained culture solution is shown in the upper panel of FIG.

  As an internal standard, stearyl alcohol (manufactured by Nacalai Tesque) was added to 5 ml of the obtained culture broth to a final concentration of 3.7 mM. To 6 ml of the resulting mixture, 5 ml of 1,1,1,3,3,3-hexafluoro-2-propanol was added to extract polyethylene terephthalate and degradation products derived from the polyethylene terephthalate. The obtained culture solution-1,1,1,3,3,3-hexafluoro-2-propanol two-phase system was centrifuged at 2385 × g for 10 minutes to obtain a supernatant. Thereafter, the obtained extract was dried using an evaporator.

  To the obtained product, 5 ml of 1,1,1,3,3,3-hexafluoro-2-propanol was added. The obtained product was analyzed by TLC / FID in the same manner as in Example 2. The results are shown in the lower panel of FIG. In the figure, the decomposition rate is shown as 100% when the peak area of the resin is zero.

  As a result, it is understood that polyethylene terephthalate can be decomposed by the microorganism group A, as shown in FIG.

  According to the present invention, the polyester compound and / or the styrene acrylic compound at the time of processing of waste represented by toner and the like can be processed at low cost and with low influence on the environment. In addition, according to the present invention, it is possible to recover metals or pigments in waste at low cost and with low impact on the environment.

FIG. 1 is a diagram showing a screening result of microorganisms that can degrade a polyester compound or a styrene acrylic compound. Panel (A) shows the result of observing a polyester compound and panel (B) after culturing a medium containing a styrene acrylic compound for 1 week.

FIG. 2 is a diagram showing the results of examining the influence of the number of passages of microorganism group A and microorganism group F. In the figure, circles indicate the degradation rate of the polyester compound by the microorganism group A, and triangles indicate the decomposition rate of the polyester compound by the microorganism group F. In the figure, the square marks indicate the decomposition rate of the styrene acrylic compound by the microorganism group A. In the figure, the decomposition rate is shown as 100% when the peak area of the resin is zero.

FIG. 3 is a diagram showing the results of examining the influence of the nitrogen source on the decomposition rate of the styrene acrylic compound by the microorganism group A. Panel (A) shows the difference in culture time, and panel (B) shows the difference depending on the number of passages. In the figure, the decomposition rate is shown as 100% when the peak area of the resin is zero.

FIG. 4 is a graph showing the results of examining the effect of yeast extract on the degradation rate of styrene acrylic compounds by microorganism group A. Panel (A) shows the number of passages and panel (B) shows the difference depending on the yeast extract concentration. In the figure, the decomposition rate is shown as 100% when the peak area of the resin is zero.

FIG. 5 is a diagram showing the morphological properties of isolated microorganisms.

FIG. 6 is a diagram showing the results of examining the degradation rate of polyesters by isolated microorganisms and combinations thereof, and microorganism groups. Panel (A) shows the degradation rate of each isolated microorganism alone. Panel (B) is a diagram showing the results of comparing the degradation rate by the combination of isolated microorganisms with the degradation rate by the microorganism group. In the figure, A represents the microorganism group A, R represents the microorganism mixture product R, F represents the microorganism group F, D represents the microorganism mixture product D, and RD represents the microorganism mixture product RD. In the figure, the decomposition rate is shown as 100% when the peak area of the resin is zero.

FIG. 7 is a diagram showing the results of examining the degradation of polyethylene terephthalate by microorganisms. In the figure, the decomposition rate is shown as 100% when the peak area of the resin is zero.

  SEQ ID NO: 1 is the sequence of forward primer 27f.

  SEQ ID NO: 2 is the sequence of reverse primer 1492r.

  SEQ ID NO: 3 is the sequence of forward primer f1L.

  SEQ ID NO: 4 is the sequence of forward primer f2L.

  SEQ ID NO: 5 is the sequence of forward primer f3L.

  SEQ ID NO: 6 is the sequence of reverse primer r1L.

  SEQ ID NO: 7 is the sequence of reverse primer r2L.

  SEQ ID NO: 8 is the sequence of reverse primer r3L.

  SEQ ID NO: 9 is the sequence of reverse primer r4L.

  SEQ ID NO: 10 is the sequence of reverse primer rE1L.

Claims (4)

A microbial culture Do that contain at least two microorganisms exhibiting biodegradability of the polyester compound and / or a styrene acrylic compounds, at least two kinds of microorganisms
i) a microorganism consisting of Pseudomonas aeruginosa, Clavibacter xyli subsp. cynodontis and a microorganism consisting of Alkagenes sp. or a mixture of Alcaligenes sp.
ii) A microbial culture, which is a microbial mixture consisting of Pseudomonas aeruginosa and Sphingomonas paucimobilis.   A composition for decomposing a polyester compound and / or a styrene acrylic compound, comprising the microorganism culture according to claim 1.   A polyester compound and / or a product or waste containing a polyester compound and / or a styrene acrylic compound is contacted with the microorganism culture according to claim 1 or the degradation composition according to claim 2. A method for decomposing styrene acrylic compounds.   (A) A waste comprising a polyester compound and / or a styrene acrylic compound and a metal or a pigment, and (B) the microorganism culture according to claim 1 or the decomposition composition according to claim 2 are brought into contact with each other. A method for recovering metals or pigments in waste.

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