JP4524801B2 - Method for producing fermented brown rice and method for producing fermented germinated brown rice - Google Patents
Method for producing fermented brown rice and method for producing fermented germinated brown rice Download PDFInfo
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本発明は、栄養価が高く、健康の維持・増進に有効な玄米発酵物、その製造方法及びそれを含む飲食品並びに発酵発芽玄米の製造方法及びその発酵発芽玄米に関する。 The present invention relates to a fermented brown rice product that has high nutritional value and is effective for maintaining and promoting health, a method for producing the same, a food and drink containing the same, a method for producing fermented germinated brown rice, and a fermented germinated brown rice.
日本人の主食である米の生体へ及ぼす有効な機能について、種々の研究がなされており、近年の健康食品ブームを反映して機能性を有する加工米が開発、市販されている。例えば、非特許文献1には、血清コレステロール低下作用を有するγ−オリザノール等の玄米成分を含む加工米が開示されている。また、特許文献1には、米胚芽,胚芽を含む米糠,胚芽米,小麦胚芽及び小麦胚芽を含む麸の中の少なくとも1種をpH2.5〜7.5かつ50℃以下の条件で水に浸漬して得たγ−アミノ酪酸を富化した食品素材が開示されている。このγ−アミノ酪酸(GABA)は、玄米を温水に適当な時間浸漬することにより発芽させた発芽玄米に多く含まれており、動物の血圧を下げる機能等を有していることが知られている。 Various studies have been conducted on effective functions of rice, which is a staple food for Japanese people, on living organisms, and processed rice having functionality reflecting the recent health food boom has been developed and marketed. For example, Non-Patent Document 1 discloses processed rice containing brown rice components such as γ-oryzanol having a serum cholesterol lowering effect. Patent Document 1 discloses that at least one of rice germ, rice bran containing germ, germ rice, wheat germ and straw containing wheat germ is submerged in water at pH 2.5 to 7.5 and 50 ° C. or less. A food material enriched with γ-aminobutyric acid obtained by immersion is disclosed. This γ-aminobutyric acid (GABA) is abundantly contained in germinated brown rice germinated by immersing brown rice in warm water for an appropriate time, and is known to have a function of lowering blood pressure of animals. Yes.
一方、生きた状態で腸管に到達し、宿主に対して有効な保健効果を示す菌は、プロバイオティクス菌と定義され、ラクトバチルス属の乳酸菌やビフィドバクテリウム属菌等が注目されている。そして、これらのプロバイオティクス菌に関しては、整腸効果や消化吸収能の改善,血清コレステロールの低減,抗腫瘍効果,免疫賦活化作用等数多くの保健効果について報告されている。例えば、特許文献2には、乳酸菌またはその処理物を有効成分として含有することを特徴とする免疫賦活作用を有する炊飯用組成物が開示されている。また、特許文献3には、発芽米を乳酸菌発酵して得られる乳酸菌発酵発芽米を配合した乳化剤及び化粧料が開示されている。 On the other hand, bacteria that reach the intestinal tract in a living state and show effective health effects on the host are defined as probiotic bacteria, and lactobacilli of the genus Lactobacillus and Bifidobacterium are attracting attention . And about these probiotic bacteria, many health effects, such as an intestinal regulation effect and digestion absorption improvement, reduction of serum cholesterol, an antitumor effect, and an immunostimulatory effect, are reported. For example, Patent Document 2 discloses a composition for cooking rice having an immunostimulatory action characterized by containing lactic acid bacteria or a processed product thereof as an active ingredient. Patent Document 3 discloses an emulsifier and a cosmetic containing lactic acid bacteria fermented germinated rice obtained by fermenting germinated rice with lactic acid bacteria.
また、乳酸菌は、様々な食品に存在し、有機酸やバクテリオシンなどの抗菌性物質を生産し、最も安全な微生物として食品と共に長年にわたって食べ続けられてきたことからバイオプリザベーションの食品の応用例として、乳製品,食肉製品,水産製品等で幅広く利用されている(非特許文献2)。例えば、特許文献4及び5には、発芽した玄米を乳酸菌発酵処理した後、熱水洗浄して乳酸菌発酵処理を停止させた後、水切り後乾燥させた玄米を洗米後、浸漬水に浸漬し、次いで浸漬水から取り出し、炊飯直前に炊き水を添加して炊飯後、必要に応じて蒸らした後、無菌設備中でガスバリヤー性耐熱容器中に充填し、完全に密封して無菌玄米ごはんを製造する際に、炊飯後の玄米ごはんのpHが4〜4.8となるように調整する、無菌玄米ごはんの製造方法が開示されている。
上述のように、保健効果を有する乳酸菌を、発芽させた玄米に利用する試みは行われているものの、常法による発芽玄米製造法の発芽工程において、玄米自身の代謝作用の他、玄米の外周部や水に含まれている雑菌の繁殖などにより、発酵状態となり、玄米に発酵臭や異臭が残留する虞があるため、発芽した玄米を、熱水洗浄して発芽した玄米の外周面に付着した雑菌を殺菌するなどの殺菌工程が必要であり、雑菌を除去するのに手間がかかるといった問題があった。また、乳酸菌を玄米の状態から利用する試みは行われていなかった。 As described above, although attempts have been made to use lactic acid bacteria having health effects in germinated brown rice, in the germination process of the conventional germination brown rice production method, in addition to the metabolic action of brown rice itself, the periphery of brown rice Fermented by miscellaneous bacteria contained in the head and water, the fermented odor and off-flavor may remain in the brown rice, so the germinated brown rice adheres to the outer surface of the germinated brown rice after washing with hot water There is a problem that a sterilization process such as sterilization of various germs is necessary, and it takes time to remove the germs. In addition, no attempt has been made to utilize lactic acid bacteria from the state of brown rice.
そこで、本発明は、以上のとおりの事情に鑑みてなされたものであって、栄養価が高く、健康の維持・増進に有効な玄米発酵物及びそれを含む飲食品並びに発酵発芽玄米を提供することを目的とする。また、本発明は、玄米や発芽玄米における雑菌を容易に抑制可能な玄米発酵物の製造方法並びに発酵発芽玄米の製造方法を提供することを目的とする。 Then, this invention is made | formed in view of the situation as mentioned above, Comprising: Fermented brown rice with high nutritional value and effective in maintenance and promotion of health, food and drink containing the same, and fermented germinated brown rice For the purpose. Moreover, an object of this invention is to provide the manufacturing method of the brown rice fermented material which can suppress easily the germs in brown rice and germinating brown rice, and the manufacturing method of fermented germinating brown rice.
上記課題を鑑みて鋭意検討した結果、玄米を、乳酸菌を含有する浸漬液に浸漬して発酵させることにより、この玄米発酵物の発酵玄米及びその発酵液において乳酸菌が増殖し、乳酸菌により有害細菌の生育を抑制し、さらに発酵玄米が発芽することを見出し、本発明に想到した。 As a result of diligent examination in view of the above problems, by immersing brown rice in an immersion liquid containing lactic acid bacteria and fermenting it, lactic acid bacteria proliferate in the fermented brown rice of this brown rice fermented product and its fermentation liquid, and The inventors have found that fermented brown rice germinates while suppressing growth, and have arrived at the present invention .
本発明の請求項1記載の玄米発酵物の製造方法は洗米した発芽前の玄米を、30〜40℃の条件下で乳酸菌を含む浸漬液中に浸漬して発酵及び発芽させ、前記玄米に乳酸菌を付着させることを特徴とする。 The process according to claim 1, brown rice fermentation product according to the present invention the brown rice preemergence was rinsed rice was immersed in the immersion solution containing lactic acid bacteria fermented and germinated under conditions of 30 to 40 ° C., the lactic acid bacteria to the brown rice It is characterized by adhering .
本発明の請求項2記載の発酵発芽玄米の製造方法は、洗米した発芽前の玄米を、乳酸菌を含む浸漬液中に浸漬して発酵及び発芽させ、蒸煮処理した後、乾燥処理することを特徴とする。 The method for producing fermented germinated brown rice according to claim 2 of the present invention is characterized in that the washed brown rice before germination is immersed in an immersion liquid containing lactic acid bacteria, fermented and germinated, steamed, and then dried. And
本発明の請求項3記載の発酵発芽玄米の製造方法は、30〜40℃の条件下で前記乳酸菌を含む浸漬液中に浸漬して発酵されることを特徴とする。 The method for producing fermented germinated brown rice according to claim 3 of the present invention is characterized in that the fermented germinated brown rice is fermented by being immersed in an immersion liquid containing the lactic acid bacteria under a condition of 30 to 40 ° C.
本発明の請求項4記載の発酵発芽玄米の製造方法は、糊化度を60〜85%にさせることを特徴とする。 The method for producing fermented germinated brown rice according to claim 4 of the present invention is characterized in that the degree of gelatinization is 60 to 85% .
本発明の請求項1記載の玄米発酵物の製造方法によれば、乳酸菌を増殖させ、乳酸菌により有害細菌の生育を抑制し、且つ、健康の維持・増進に有効な玄米発酵物を提供できる。 According to the manufacturing method of claim 1 rice fermented product according the present invention, grown lactic acid bacteria to suppress the growth of harmful bacteria by lactic acid bacteria, and can provide an effective brown rice fermentation product to maintenance and promotion of health.
本発明の請求項2記載の発酵発芽玄米の製造方法によれば、乳酸菌により有害細菌の生育を抑制し、さらに玄米から溶出される栄養成分で乳酸菌が増殖するので、乳酸菌による保健効果を有し、健康の維持・増進に有効であり、栄養価が一層高い発酵発芽玄米が得られる。 According to the method for producing fermented germinated brown rice according to claim 2 of the present invention, the growth of harmful bacteria is suppressed by lactic acid bacteria, and further, the lactic acid bacteria are propagated by nutrient components eluted from the brown rice. Fermented and germinated brown rice that is effective in maintaining and promoting health and having a higher nutritional value can be obtained.
本発明の請求項3記載の発酵発芽玄米の製造方法によれば、乳酸菌を増殖させ、乳酸菌により有害細菌の生育を抑制し、且つ、健康の維持・増進に有効な発酵発芽玄米を提供できる。 According to the method for producing fermented germinated brown rice according to claim 3 of the present invention, fermented germinated brown rice can be provided which is capable of growing lactic acid bacteria, suppressing the growth of harmful bacteria by the lactic acid bacteria, and effective in maintaining and promoting health.
本発明の請求項4記載の発酵発芽玄米の製造方法によれば、特に、食味に優れた発酵発芽玄米を提供できる。 According to the method for producing fermented germinated brown rice according to claim 4 of the present invention, it is possible to provide fermented germinated brown rice particularly excellent in taste .
以下、本発明を詳細に説明する。 Hereinafter, the present invention will be described in detail.
本発明に用いられる玄米としては、特に限定されないが、例えば、粳米の玄米,糯米の玄米,色素米の玄米,或いは、5分づき米,7分づき米等の玄米から精米に至る種々の状態の米が挙げられる。 Although it does not specifically limit as brown rice used for this invention, For example, various states ranging from brown rice, such as brown rice of sticky rice, brown rice of sticky rice, brown rice of pigmented rice, or rice for 5 minutes, rice for 7 minutes Of rice.
本発明に用いられる乳酸菌としては、例えば、ラクトバチルス カゼイ(Lactobacilluscasei),ラクトバチルス プランタラム(L.plantarum),ラクトバチルス ファーメンタム(L.fermentum),ラクトバチルス アシドフィラス(L.acidophilus),ラクトバチルスガセリ(L.gasseri),ラクトバチルス パラカゼイ(L.paracasei),ラクトバチルスラムノーサス(L.rhamnosus),ラクトバチルス ヘルバティカス(L.helveticus)などのラクトバチルス属が挙げられる。また、本発明においては、1種類の乳酸菌を単独で用いてもよいし、複数の乳酸菌を任意に組み合わせてもよい。 Examples of lactic acid bacteria used in the present invention include Lactobacillus casei, Lactobacillus plantarum, L. fermentum, L. fermentum, L. acidophilus, and Lactobacillus gasseri. (L. gasseri), Lactobacillus paracasei, L. rhamnosus, Lactobacillus helvaticus (L. helveticus) and the like. In the present invention, one type of lactic acid bacterium may be used alone, or a plurality of lactic acid bacteria may be arbitrarily combined.
乳酸菌スターターの培地としては、乳酸菌用のMRS培地(組成:プロテオースペプトン1%、牛肉エキス1%、酵母エキス0.5%、ブドウ糖2%、Tween 80 0.1%、クエン酸アンモニウム0.5%、硫酸マグネシウム0.01%、硫酸マンガン0.005%、リン酸二カリウム0.2%、DIFCO社製),10%脱脂粉乳培地などが挙げられる。乳酸菌スターターには、培養物をそのまま、若しくは遠心分離器で集菌した洗浄菌体が用いられる。また、乳酸菌の種類等にもよるが、例えば約107〜109cfu/mLの乳酸菌を含むことが好ましい。 As a medium for lactic acid bacteria starter, MRS medium for lactic acid bacteria (composition: proteose peptone 1%, beef extract 1%, yeast extract 0.5%, glucose 2%, Tween 80 0.1%, ammonium citrate 0.5% %, Magnesium sulfate 0.01%, manganese sulfate 0.005%, dipotassium phosphate 0.2%, manufactured by DIFCO), 10% nonfat dry milk medium, and the like. For the lactic acid bacteria starter, washed cells obtained by collecting the cultures as they are or using a centrifuge are used. Further, although depending on the type of lactic acid bacteria, it is preferable to contain, for example, about 10 7 to 10 9 cfu / mL of lactic acid bacteria.
本発明の玄米発酵物の製造方法は、例えば以下のようにして行われる。まず、洗米した玄米を、30〜40℃、好ましくは35〜37℃、より好ましくは約37℃の条件下で、該玄米の総重量の0.1〜1重量%の乳酸菌を含む溶液中に、約6時間以上浸漬して発酵させ、前記玄米に乳酸菌を付着させた発酵玄米とその発酵液を含む玄米発酵物を得ることができる。発酵温度が30℃より低い場合、乳酸菌以外の菌が増殖するため好ましくなく、また40℃を超える場合、発酵温度が高いため乳酸菌発酵ができず好ましくない。玄米を、乳酸菌を含む溶液中に約6時間浸漬すると、玄米の表面に乳酸菌が付着し、付着した乳酸菌は玄米から溶出される栄養成分で増殖でき乳酸菌発酵を行うことが判明した。したがって、乳酸菌発酵の約6時間後には、発酵液の一部又は全部を取り出して新たな水と交換することで、発酵時間の調整が可能である。このように発酵液を新たな水と交換することによって、乳酸菌発酵により生成される乳酸の量の増加によるpHの低下を防ぐことができる。さらに、発酵玄米を発芽させるには、約15〜36時間、好ましくは約17時間浸漬して発酵させることにより適度に発芽させることができる。また、この発酵玄米は、乳酸菌発酵及び乳酸菌を含む浸漬液中での浸漬処理により軟化し、また浸漬液中で発芽することによってさらに軟化する。 The manufacturing method of the brown rice fermented material of this invention is performed as follows, for example. First, the washed brown rice is placed in a solution containing lactic acid bacteria of 0.1 to 1% by weight of the total weight of the brown rice under conditions of 30 to 40 ° C., preferably 35 to 37 ° C., more preferably about 37 ° C. The fermented brown rice containing the fermented brown rice and the fermented liquid obtained by immersing and fermenting for about 6 hours or more and attaching lactic acid bacteria to the brown rice can be obtained. When the fermentation temperature is lower than 30 ° C., bacteria other than lactic acid bacteria grow, which is not preferable, and when it exceeds 40 ° C., fermentation temperature is high, and lactic acid bacteria fermentation cannot be performed, which is not preferable. It was found that when brown rice is immersed in a solution containing lactic acid bacteria for about 6 hours, lactic acid bacteria adhere to the surface of the brown rice, and the attached lactic acid bacteria can grow with nutrients eluted from the brown rice and perform lactic acid bacteria fermentation. Therefore, about 6 hours after lactic acid bacteria fermentation, the fermentation time can be adjusted by taking out part or all of the fermentation broth and replacing it with fresh water. Thus, by exchanging the fermentation broth with fresh water, it is possible to prevent a decrease in pH due to an increase in the amount of lactic acid produced by lactic acid bacteria fermentation. Furthermore, in order to germinate fermented brown rice, it can be made to germinate appropriately by immersing and fermenting for about 15 to 36 hours, preferably about 17 hours. The fermented brown rice is softened by lactic acid bacteria fermentation and an immersion treatment in an immersion liquid containing lactic acid bacteria, and further softened by germination in the immersion liquid.
洗米処理方法としては、例えば水や温水等により行うことができ、玄米の外周部に付着している雑菌を洗い流すことができる。さらに、乳酸菌による乳酸菌発酵を導入することにより、有害細菌の生育を抑制することができる。 As the rice washing treatment method, for example, water or warm water can be used, and various bacteria attached to the outer peripheral portion of the brown rice can be washed away. Furthermore, the growth of harmful bacteria can be suppressed by introducing lactic acid bacteria fermentation by lactic acid bacteria.
また、本発明の玄米発酵物は、洗米した玄米を、乳酸菌を含む浸漬液中に浸漬して発酵させ、前記玄米に乳酸菌を付着させた発酵玄米とその発酵液とを含む。さらに、本発明の玄米発酵物の発酵玄米は、軟らかい食感を有する発芽した玄米である。 Moreover, the brown rice fermented product of this invention contains the fermented brown rice which fermented by immersing the washed brown rice in the immersion liquid containing a lactic acid bacterium, and making lactic acid bacteria adhere to the said brown rice, and its fermented liquid. Furthermore, the fermented brown rice of the brown rice fermented product of the present invention is a germinated brown rice having a soft texture.
本発明の玄米発酵物は、固液分離操作を行うことにより、発酵玄米とその発酵液とに分離することができる。さらに、該発酵玄米とその発酵液とを乾燥することによって、粒状態の玄米発酵物を得ることができる。或いは、該発酵玄米を耐熱容器に充填し、この耐熱容器の開口部をシールして密封し、次いでレトルト殺菌処理を行うことにより、レトルト加工米を得ることができる。固液分離操作としては、例えば遠心分離、静置分離、ろ過分離、膜分離などが挙げられ、公知の方法が適宜選択される。また、乾燥方法としては、食品の乾燥に用いられる方法であれば特に限定されず、例えば、凍結、真空、噴霧、熱風、あるいはこれらを組み合わせた乾燥方法などが挙げられる。 The brown rice fermented product of the present invention can be separated into fermented brown rice and its fermentation broth by performing solid-liquid separation operation. Further, by drying the fermented brown rice and its fermentation broth, a fermented brown rice product in a grain state can be obtained. Alternatively, retort-processed rice can be obtained by filling the fermented brown rice in a heat-resistant container, sealing and sealing the opening of the heat-resistant container, and then performing retort sterilization treatment. Examples of the solid-liquid separation operation include centrifugation, stationary separation, filtration separation, membrane separation and the like, and a known method is appropriately selected. The drying method is not particularly limited as long as it is a method used for drying foods, and examples thereof include freezing, vacuum, spraying, hot air, or a combination of these.
本発明の玄米発酵物は、粉末状態又は粒状態、液体状態に加工でき、旨味、渋味、苦味、塩味などを呈する各種物質ともよく調和し、広く一般の飲食品、例えば、米菓、和菓子、洋菓子、氷菓、調味料、畜肉加工品、魚肉・水産加工品、乳・卵加工品、野菜加工品、果実加工品、穀類加工品、飲料など広範に利用可能である。本発明の玄米発酵物は、乳酸菌発酵により乳酸菌を豊富に含み、玄米が発芽し栄養価が高く健康の維持・増進に有効であるので、本発明の玄米発酵物を上記飲食品に添加することによって、健康の維持・増進に有効な健康食品とすることができる。 The brown rice fermented product of the present invention can be processed into a powder state, a grain state, or a liquid state, and is well harmonized with various substances exhibiting umami, astringency, bitterness, salty taste, and the like, and is widely used in general foods and beverages such as rice confectionery and Japanese confectionery. , Western confectionery, ice confectionery, seasonings, processed meat products, processed fish / fishery products, processed milk / eggs, processed vegetables, processed fruits, processed cereals, and beverages. Since the brown rice fermented product of the present invention contains abundant lactic acid bacteria by lactic acid bacteria fermentation, and the brown rice germinates and has a high nutritional value and is effective in maintaining and promoting health, the fermented brown rice product of the present invention should be added to the food and drink. Therefore, it is possible to obtain a health food effective for maintaining and promoting health.
さらに、本発明の玄米発酵物の発酵玄米は、乳酸菌発酵や乳酸菌を含む浸漬液中での浸漬処理により、また発芽した玄米であるため玄米外層部が軟らかく、精白米と同様に炊飯器で炊くことができ、精白米に近い軟らかさで食することができるため、健康の維持・増進に有効な発芽玄米としても提供することができる。 Further, the fermented brown rice of the brown rice fermented product of the present invention is a brown rice that has been germinated by immersing in a dipping solution containing lactic acid bacteria fermentation or lactic acid bacteria. In addition, since it can be eaten with a softness close to polished rice, it can also be provided as germinated brown rice that is effective in maintaining and promoting health.
そして、後述する実施例5のように、洗米した玄米を、乳酸菌を含む浸漬液中に浸漬して発酵及び発芽させ、得られた発酵発芽玄米をレトルト殺菌を行い、レトルト加工米とすることができる。 And like Example 5 mentioned later, the wash | cleaned brown rice is immersed in the immersion liquid containing a lactic acid bacterium, is fermented and germinated, the obtained fermented germinated brown rice is retort-sterilized, and it is set as a retort processed rice. it can.
上記レトルト加工米が所謂「ウエット品」であるのに対し、所謂「ドライ品」に例について説明すると、洗米した玄米を、乳酸菌を含む浸漬液中に浸漬して発酵及び発芽させ、これにより得られた玄米発酵物である発酵発芽玄米を、蒸煮処理した後、乾燥処理した発酵発芽玄米を炊飯して食することができる。また、この発酵発芽玄米は、上述したように広く一般の飲食品に適用できる。 The retort-processed rice is a so-called “wet product”, whereas the so-called “dry product” will be described as an example. The washed rice is soaked in an immersion liquid containing lactic acid bacteria and fermented and germinated. The fermented germinated brown rice, which is a fermented brown rice product, is steamed and then dried and fermented germinated brown rice can be cooked and eaten. Moreover, this fermented germinated brown rice can be widely applied to general food and drink as described above.
具体的には、上述した玄米発酵物の製造方法により得られた発酵発芽玄米を、固液分離操作を行うことにより、発酵発芽玄米とその発酵液とに分離し、粒状態になる程度に表面の発酵液を除去する。その固液分離操作としては、上述した各種の方法が適宜選択される。 Specifically, the fermented germinated brown rice obtained by the above-described method for producing fermented brown rice is separated into fermented germinated brown rice and its fermented liquid by performing a solid-liquid separation operation, and the surface is brought into a grain state. Remove the fermentation broth. As the solid-liquid separation operation, the various methods described above are appropriately selected.
前記表面の発酵液を除去した発酵発芽玄米を、蒸煮処理した後、乾燥処理することにより、「ドライ品」の製品としての発酵発芽玄米が得られる。この発酵発芽玄米は、糊化度を60〜85%とすることが食味の点から好ましく、また、水分含量は、12〜16%程度とすることが好ましい。尚、前記糊化度は、βアミラーゼ・プルラナーゼ法(BAP法)により測定した。蒸煮処理においては、100℃で5〜30分間、セイロで蒸した際の糊化度は10〜85%であり、時間を調整して60〜85%の糊化度の発酵発芽玄米を製造した。後述する実験例4のように、発酵発芽玄米は、糊化度が50%程度では、一般の玄米や発芽玄米と比較して食味の面で大きな違いが見られず、一方、糊化度が85%を越すと、吸水率が多くなるため、軟らかくなりすぎて食味がよくなく、糊化度が60〜85%で食味に優れたものが得られた。 The fermented germinated brown rice from which the fermented liquid on the surface has been removed is steamed and then dried to obtain fermented germinated brown rice as a “dry product” product. The fermented germinated brown rice preferably has a gelatinization degree of 60 to 85% from the viewpoint of taste, and preferably has a water content of about 12 to 16%. The gelatinization degree was measured by the β amylase / pullulanase method (BAP method). In the steaming treatment, the degree of gelatinization when steamed in a salo for 5 to 30 minutes at 100 ° C. is 10 to 85%, and the fermented germinated brown rice having a degree of gelatinization of 60 to 85% was prepared by adjusting the time. . As in Experimental Example 4 to be described later, the fermented germinated brown rice has a gelatinization degree of about 50%, and there is no significant difference in taste compared to general brown rice or germinated brown rice. If it exceeds 85%, the water absorption increases, so it becomes too soft and the taste is not good, and a paste with a gelatinization degree of 60 to 85% and an excellent taste is obtained.
次に、本発明の効果を確認する検査方法について以下の試験例で説明する。 Next, an inspection method for confirming the effect of the present invention will be described in the following test examples.
(試験例1)乳酸菌スターターの培養:日本乳業技術協会より購入したL.casei L−14を用いた。スターター用培地は、MRS培地を用い、37℃、24時間培養し、遠心分離後、洗浄して乳酸菌の菌数を約109cfu/mLに調整した。 (Test Example 1) Cultivation of lactic acid bacteria starter: L. lactic acid bacteria purchased from the Japan Milk Industry Association. casei L-14 was used. The starter medium was an MRS medium, cultured at 37 ° C. for 24 hours, centrifuged and washed to adjust the number of lactic acid bacteria to about 10 9 cfu / mL.
次に、玄米3品種(新潟県産のコシヒカリ,ミルキークィーン,こしいぶき)を流水で2分間水洗いした後、この水洗いした玄米を、該玄米の総重量と同量の蒸留水(約35℃)と該玄米の総重量に対して0.1重量%の乳酸菌スターターとを均一に懸濁した浸漬液に浸漬し、37℃で17時間発酵させ、玄米発酵物を得た。さらに、固液分離操作を行い、玄米発酵物を、発酵玄米と発酵液とに分離した。ここで得られた発酵玄米を流水により30秒間4回洗浄し、300粒の発酵玄米を顕微鏡により形態観察し、発酵玄米の発芽率を算出した。その後、発酵玄米の10倍量の蒸留水を添加し、ミキサーで破砕し、発酵玄米のpHおよび菌数を測定した。また、玄米発酵物の発酵液は、そのままの状態で発酵液のpHおよび菌数を測定した。 Next, three brown rice varieties (Koshihikari, milky queen, Koishibuki from Niigata Prefecture) were washed with running water for 2 minutes, and then the washed brown rice was distilled water (about 35 ° C) in the same amount as the total weight of the brown rice. And 0.1% by weight of lactic acid bacteria starter with respect to the total weight of the brown rice were immersed in an immersion liquid and fermented at 37 ° C. for 17 hours to obtain a brown rice fermentation product. Furthermore, solid-liquid separation operation was performed and the brown rice fermented material was isolate | separated into fermented brown rice and fermentation broth. The fermented brown rice obtained here was washed 4 times with running water for 30 seconds, and 300 grains of fermented brown rice were observed with a microscope to calculate the germination rate of the fermented brown rice. Then, 10 times the amount of distilled water of fermented brown rice was added and crushed with a mixer, and the pH and the number of bacteria of the fermented brown rice were measured. Moreover, the fermentation liquid of the brown rice fermented product measured the pH and the number of bacteria of the fermentation liquid as it was.
なお、微生物分析としては、玄米を滅菌した生理食塩水と一緒に無菌袋に入れ、ストマッカー(オルガノ社製)で2分間破砕した。玄米破砕液及び発酵液は1/10ずつ段階希釈を行い、菌数測定に用いた。 For microbial analysis, brown rice was placed in a sterile bag together with sterilized physiological saline and crushed with a stomacher (manufactured by Organo) for 2 minutes. The brown rice crushed liquid and the fermented liquid were serially diluted by 1/10 each and used for the bacterial count measurement.
各々の菌における菌数は、以下の条件下の培地におけるコロニー形成数(cfu/mL又はcfu/g)をカウントして測定した。一般生菌数は、標準寒天培地(日水製薬社製)を用いて48時間培養;乳酸菌数は、BCP加プレートカウントアガール(日水製薬社製)を用いて48〜72時間培養;ラクトバチルス(Lactobacillus)数は、LBSagar(BBL社製)を用いて48時間嫌気培養(BBL社製:GasPack);グラム陽性菌数は変法フェネチルアルコール培地(標準寒天培地に0.3%のβ−フェネチルアルコール(関東化学社製)を含む)を用いて48時間培養;グラム陰性菌数は、CVT寒天培地(日水製薬社製)を用いて48時間培養;シュードモナス(Pseudomonas)数は、Pseudomonas Isolation agar(DIFCO社製)を用いて48〜72時間培養;大腸菌群数は、デソキシコレート培地(日水製薬社製)を用いて24時間培養;バチルスセレウス(Bcilllus cereus)数は、NGKG寒天基礎培地(日水製薬社製)を用いて24時間培養;黄色ブドウ球菌数は卵黄加マンニット食塩培地(日水製薬社製)を用いて36時間培養;培養温度は各々37℃。 The number of bacteria in each bacterium was measured by counting the number of colonies formed (cfu / mL or cfu / g) in the medium under the following conditions. General viable cell count is cultured for 48 hours using standard agar medium (manufactured by Nissui Pharmaceutical); lactic acid bacteria count is cultured for 48 to 72 hours using BCP-added plate count agar (manufactured by Nissui Pharmaceutical); Lactobacillus (Lactobacillus) number is anaerobic culture using LBSagar (manufactured by BBL) (BBL company: GasPack); the number of gram positive bacteria is modified phenethyl alcohol medium (0.3% β-phenethyl on standard agar medium) Cultured for 48 hours using alcohol (including Kanto Chemical Co., Inc.); Gram-negative bacteria were cultured for 48 hours using CVT agar medium (manufactured by Nissui Pharmaceutical); Pseudomonas number was Pseudomonas Isolation agar Culturing for 48 to 72 hours using DIFCO (manufactured by DIFCO); the number of coliforms is 24 using deoxycholate medium (manufactured by Nissui Pharmaceutical). Time culture; B. cereus number is cultivated for 24 hours using NGKG agar basal medium (manufactured by Nissui Pharmaceutical Co., Ltd.); Culture for 36 hours; culture temperature is 37 ° C. each.
また、発酵液及び発酵玄米の乳酸菌以外の菌数は、標準寒天培地より求めた菌数からLBS agarより求めた菌数を減じる方法又はBCP加プレートカウントアガールでハローを形成しないコロニー数を算出する方法により求めた。 The number of bacteria other than lactic acid bacteria in the fermentation broth and fermented brown rice is calculated by subtracting the number of bacteria obtained from LBS agar from the number of bacteria obtained from the standard agar medium, or the number of colonies that do not form halos with BCP-added plate count agar. Determined by the method.
標準寒天培地で生育した菌は、グラム染色後の細胞観察,カタラーゼ及びオキシダーゼの有無、0−Fテストで同定した。 Bacteria grown on the standard agar medium were identified by cell observation after Gram staining, presence or absence of catalase and oxidase, and 0-F test.
玄米3品種の水洗前後の菌数及び乳酸菌発酵後の発酵玄米の菌数の結果を表1に示す。 Table 1 shows the results of the number of bacteria before and after washing with three types of brown rice and the number of bacteria in fermented brown rice after fermentation with lactic acid bacteria.
表1に示すように、玄米3品種に付着している菌数はそれぞれ約106(cfu/g)以上であり、水洗いすることで1オーダー程度低下した。また、この水洗いした玄米を、玄米と同重量の蒸留水に乳酸菌スターターを玄米に対して0.1%添加して均一に懸濁した浸漬液に浸漬させ、37℃で17時間発酵させて得られた玄米発酵物の発酵玄米の全菌数の増加が見られた。また、発酵玄米の全菌数と、ラクトバチルス数はほぼ同一であった。この結果から、発酵玄米の菌のほとんどがラクトバチルスであることがわかる。また、品種の異なる玄米においても同様の傾向が見られた。さらに、発酵玄米の発芽率は、約100%であった。この結果から、乳酸菌の存在下でも玄米が発芽することが認められた。なお、ここで得られた発酵玄米のpHは約5.5〜6.5であり、この発酵玄米を炊飯しても酸味を感じることなく、美味しく食することができた。また、この発酵玄米は、周知の温水に浸漬させた方法により発芽させた発芽玄米よりも、軟らかい食感を有す玄米であった。 As shown in Table 1, the number of bacteria adhering to the three varieties of brown rice was about 10 6 (cfu / g) or more, and was reduced by about one order by washing with water. Further, this washed brown rice is obtained by immersing in a dipping solution in which 0.1% of lactic acid bacteria starter is added to distilled rice in the same weight as brown rice and uniformly suspended, and fermented at 37 ° C. for 17 hours. An increase in the total bacterial count of the fermented brown rice was observed. In addition, the total number of bacteria in the fermented brown rice and the number of Lactobacillus were almost the same. From this result, it can be seen that most of the germs of fermented brown rice are Lactobacillus. The same tendency was also observed in brown rice with different varieties. Furthermore, the germination rate of fermented brown rice was about 100%. From this result, it was confirmed that brown rice germinated even in the presence of lactic acid bacteria. In addition, pH of the fermented brown rice obtained here is about 5.5-6.5, and even if this fermented brown rice was cooked, it was able to eat deliciously without feeling sour. Moreover, this fermented brown rice was a brown rice having a softer texture than germinated brown rice germinated by a method of immersing in well-known warm water.
(試験例2)
玄米(新潟県産のコシヒカリ)と浸漬水の配合比、乳酸菌スターターの添加量、及び発酵温度をそれぞれ以下のように設定した以外は試験例1と同様にして、玄米発酵物を得た。配合比(玄米:浸漬水)、乳酸菌スターターの添加量、温度;1:1、0.1%、25℃;1:1、0.1%、30℃;1:1、0.1%、37℃;1:1、1.0%、37℃;1:10、0.1%、37℃;1:30、0.1%、37℃;1:1、0%、37℃。
(Test Example 2)
A brown rice fermented product was obtained in the same manner as in Test Example 1 except that the blending ratio of brown rice (Koshihikari produced in Niigata Prefecture) and immersion water, the addition amount of lactic acid bacteria starter, and the fermentation temperature were set as follows. Mixing ratio (brown rice: immersion water), addition amount of lactic acid bacteria starter, temperature; 1: 1, 0.1%, 25 ° C; 1: 1, 0.1%, 30 ° C; 1: 1, 0.1%, 37 ° C; 1: 1, 1.0%, 37 ° C; 1:10, 0.1%, 37 ° C; 1:30, 0.1%, 37 ° C; 1: 1, 0%, 37 ° C.
玄米発酵物(発酵玄米および発酵液)のpHと菌数を表2に示す。 Table 2 shows the pH and the number of bacteria of the fermented brown rice (fermented brown rice and fermentation broth).
表2に示すように、発酵温度が25℃や30℃の場合より、37℃の場合において、pHの低下および乳酸菌の増加が発酵液及び発酵玄米で見られ、乳酸菌以外の菌数(その他菌数)の抑制も発酵液及び発酵玄米で見られた。この結果より、乳酸菌を添加した影響が示唆される。乳酸菌スターターの添加量を1%に増やしてもpH及び菌数ともに大きな変化は見られなかった。浸漬液中の水の量を増やすと発酵液のラクトバチルス数の減少及びその他の菌数の増加が見られ、発酵玄米ではラクトバチルス数は同程度であるが、その他の菌の増加が見られた。乳酸菌を添加していない対照区では、浸漬液及び発酵玄米でその他菌数の増加が見られた。 As shown in Table 2, a decrease in pH and an increase in lactic acid bacteria were observed in the fermented liquid and fermented brown rice when the fermentation temperature was 37 ° C. than when the fermentation temperature was 25 ° C. or 30 ° C., and the number of bacteria other than lactic acid bacteria (other bacteria Number) was also observed in the fermentation broth and fermented brown rice. This result suggests the effect of adding lactic acid bacteria. Even when the addition amount of the lactic acid bacteria starter was increased to 1%, neither pH nor the number of bacteria was significantly changed. When the amount of water in the immersion liquid is increased, the number of Lactobacillus in the fermentation liquid is decreased and the number of other bacteria is increased, while the number of Lactobacillus is the same in fermented brown rice, but the number of other bacteria is increased. It was. In the control group to which lactic acid bacteria were not added, the number of other bacteria increased in the immersion liquid and fermented brown rice.
(試験例3)
表2に示したように、乳酸菌スターターを添加しなかった場合に、乳酸菌以外の菌の増殖が見られたので、選択培地を用いて玄米,乳酸菌添加(乳酸菌を含む浸漬液で発酵させた発酵玄米),無添加(乳酸菌を含まない浸漬液で浸漬させた玄米)のそれぞれの菌叢を調べた。なお、乳酸菌スターターは、上記試験例1と同様のものを用い、玄米(新潟県産のコシヒカリ)と浸漬水の配合比、乳酸菌スターターの添加量、及び発酵温度・発酵時間を以下のように設定した;1:1、0.1%、37℃、17時間。結果を表3に示す。
(Test Example 3)
As shown in Table 2, since growth of bacteria other than lactic acid bacteria was observed when no lactic acid bacteria starter was added, brown rice and lactic acid bacteria were added using a selective medium (fermented with an immersion liquid containing lactic acid bacteria) The bacterial flora of brown rice) and additive-free (brown rice soaked in a soaking solution not containing lactic acid bacteria) was examined. The lactic acid bacteria starter is the same as in Test Example 1, and the blending ratio of brown rice (Koshihikari from Niigata Prefecture) and immersion water, the amount of lactic acid bacteria starter added, and the fermentation temperature and fermentation time are set as follows: 1: 1, 0.1%, 37 ° C., 17 hours. The results are shown in Table 3.
表3より、玄米の一般生菌数は2.0×106cfu/gでありグラム陽性菌数とグラム陰性菌数を足しても、この玄米の菌数より少なく、全ての菌数は検出できなかった。一方、乳酸菌添加区では、グラム陽性菌が増殖し、ラクトバチルス数とほぼ同数の結果を示した。 From Table 3, the number of general viable bacteria in brown rice is 2.0 × 10 6 cfu / g. Even if the number of gram positive and gram negative bacteria is added, the number of bacteria is less than that in brown rice, and all the numbers of bacteria are detected. could not. On the other hand, in the lactic acid bacteria-added section, Gram-positive bacteria grew and showed almost the same number as the number of Lactobacillus.
また、玄米より分離された乳酸菌を16SrRNA遺伝子配列で同定した。LBS agarで生育した乳酸菌をMRS培地で37℃、24時間培養後、集菌洗浄した。スターターの乳酸菌と発酵玄米から無作為に分離した15個のラクトバチルスコロニーの16SrRNA遺伝子塩基配列を比較したが、全て同一であり、スターターに用いたL.caseiL−14が増殖したことを確認した。グラム陰性菌は玄米と比較して、若干の減少が見られシュードモナスは未検出であった。無添加区ではグラム陽性菌数は玄米と同程度であったが、グラム陰性菌の大きな増加、シュードモナスの増加が認められた。黄色ブドウ球菌、B.cereus及び大腸菌群は何れの玄米からも検出されなかった。 In addition, lactic acid bacteria isolated from brown rice were identified by 16S rRNA gene sequence. Lactic acid bacteria grown on LBS agar were cultured in MRS medium at 37 ° C. for 24 hours, and then collected and washed. The 16S rRNA gene base sequences of 15 Lactobacillus colonies randomly separated from the starter lactic acid bacteria and fermented brown rice were compared. It was confirmed that caseiL-14 grew. Gram-negative bacteria showed a slight decrease compared to brown rice, and Pseudomonas was not detected. In the additive-free group, the number of Gram-positive bacteria was similar to that of brown rice, but a large increase in Gram-negative bacteria and an increase in Pseudomonas were observed. S. aureus, B. Cereus and coliforms were not detected from any brown rice.
さらに、無添加区でどのような菌が増殖しているか調べるため、玄米と無添加区玄米の標準寒天培地で生育したコロニーを無作為に取り出して同定を行った。玄米より取り出した57個のコロニー中、46個がEnterobacteriaceae(腸内細菌科)であり、11個がBacillusであった。乳酸菌無添加区の玄米では、67個中全てがEnterobacteriaceaeであった。この結果より、乳酸菌無添加区では主にEnterobacteriaceaeが増殖したことが示唆された。 Furthermore, in order to investigate what kind of bacteria were growing in the non-addition zone, colonies grown on the brown rice and the standard agar medium of the non-addition zone brown rice were randomly picked and identified. Of 57 colonies taken out from brown rice, 46 were Enterobacteriaceae (Enterobacteriaceae) and 11 were Bacillus. In the brown rice in the lactic acid bacteria-free group, 67 out of 67 were Enterobacteriaceae. From this result, it was suggested that Enterobacteriaceae mainly proliferated in the lactic acid bacteria-free group.
(試験例4)
乳酸菌の違いによる玄米発酵物(発酵玄米および発酵液)のpHと菌数を調べた。乳酸菌スターターの培養:日本乳業技術協会より購入したL.caseiL−14,理化学研究所より購入したL.acidophilus JCM1132T,L.gaseri JCM1131T,L.plantarum JCM1057,L.fermentumJCM1560を用いた。スターター用培地は、MRS培地を用い、37℃、24時間培養し、遠心分離後洗浄して、乳酸菌の菌数を約109cfu/mLに調整した。なお、乳酸菌スターターは、上記試験例1と同様のものを用い、玄米(新潟県産のコシヒカリ)と浸漬水の配合比、乳酸菌スターターの添加量、及び発酵温度・発酵時間を以下のように設定した;1:1、0.1%、37℃、17時間。結果を表4に示す。
(Test Example 4)
The pH and the number of bacteria of brown rice fermented products (fermented brown rice and fermented liquid) due to differences in lactic acid bacteria were examined. Lactic acid bacteria starter culture: L. lactic acid bacteria starter caseiL-14, purchased from R.C. acidophilus JCM1132 T , L. gaseri JCM1131 T , L.M. plantarum JCM1057, L.M. fermentum JCM1560 was used. The starter medium was an MRS medium, cultured at 37 ° C. for 24 hours, centrifuged and washed to adjust the number of lactic acid bacteria to about 10 9 cfu / mL. The lactic acid bacteria starter is the same as in Test Example 1, and the blending ratio of brown rice (Koshihikari from Niigata Prefecture) and immersion water, the amount of lactic acid bacteria starter added, and the fermentation temperature and fermentation time are set as follows: 1: 1, 0.1%, 37 ° C., 17 hours. The results are shown in Table 4.
表4に示されるように、使用した全ての乳酸菌で得られた玄米発酵物のラクトバチルス数の増加が見られた。L.acidophilus JCM1132Tのみ生育が悪く、発酵液のpH低下及び乳酸菌数の増加の程度が低かったが、その他の乳酸菌では発酵液で約108cfu/g、発酵玄米で約107cfu/g以上の増殖が見られた。 As Table 4 showed, the increase in the number of Lactobacillus of the brown rice fermented material obtained with all the lactic acid bacteria used was seen. L.acidophilus JCM1132 T only growth is poor, although the degree of reduction and increase in the number of lactic acid bacteria the pH of the fermentation liquor is low, other about 10 8 cfu / g in fermented liquid is lactic acid, about 10 7 cfu / g in fermented brown rice The above proliferation was observed.
以下、本発明の実施例により具体的に説明するが、本発明はこれらの実施例に限定されるものではない。 EXAMPLES Hereinafter, the present invention will be specifically described with reference to examples, but the present invention is not limited to these examples.
(玄米発酵物の製造方法)玄米を流水で2分間水洗いした後、この水洗いした玄米を、該玄米の総重量と同量の蒸留水(約35℃)と該玄米の総重量に対して0.1重量%の乳酸菌スターターを均一に懸濁した浸漬液に浸漬させ37℃で17時間発酵させ、玄米発酵物を得た。また、固液分離操作を行うことにより、玄米発酵物から発酵発芽玄米と発酵液とを得た。 (Method for producing fermented brown rice) After washing the brown rice with running water for 2 minutes, the washed brown rice is 0% of the total weight of the brown rice with distilled water (about 35 ° C) and the total weight of the brown rice. A 1% by weight lactic acid bacteria starter was immersed in a uniformly suspended immersion liquid and fermented at 37 ° C. for 17 hours to obtain a brown rice fermented product. Moreover, fermentation germination brown rice and the fermented liquor were obtained from the brown rice fermented material by performing solid-liquid separation operation.
米菓餅生地として糯米80重量%と、実施例1で得られた発酵発芽玄米20重量%とを原料として、常法の米菓の製造方法により米菓を作製した。 A rice cracker was prepared by a conventional rice cracker manufacturing method using 80% by weight of rice cracker as a dough for rice cracker and 20% by weight of fermented germinated brown rice obtained in Example 1.
ヨーグルト90重量%と、実施例1で得られた玄米発酵物10重量%を粉砕して液状化したものを混合し、発酵飲料を作製した。 90% by weight of yogurt and 10% by weight of the fermented brown rice product obtained in Example 1 were pulverized and mixed to prepare a fermented beverage.
実施例1で得られた発酵発芽玄米200gをパウチに充填密封し、袋詰めのまま120℃、30分のレトルト殺菌を行い、レトルト加工米を作製した。 200 g of fermented germinated brown rice obtained in Example 1 was filled and sealed in a pouch, and sterilized by retort at 120 ° C. for 30 minutes while being packaged to produce retort-processed rice.
実施例1で得られた発酵発芽玄米(新潟県産のコシヒカリの玄米を発酵及び発芽したもの)を、蒸煮処理し、この蒸気処理として、発酵発芽玄米を蒸気で蒸しながら、間歇的にシャワーによる加水を行う加熱により行い、蒸煮処理を行った後、乾燥処理を行い、糊化度の異なる発酵発芽玄米を作製した。この場合、玄米を蒸し続けるといずれ表面が乾き、いくら蒸しても米に水分が浸透せず、玄米が糊化しなくなる。そこで、蒸気で蒸しながら、玄米に熱水をシャワー状にして加水することにより、表面がぬれて良く糊化し、60〜85%という比較的高い糊化度の製品を容易に製造することができ、また、加水することにより、飽和水蒸気を用いる必要がなく、即ち密封系の蒸し器でなくても、所望の糊化度の玄米を製造できる。尚、各発酵発芽玄米の水分含量は、12〜16%である。 The fermented germinated brown rice obtained in Example 1 (fermented and germinated from Koshihikari brown rice produced in Niigata Prefecture) was steamed, and as this steam treatment, the fermented germinated brown rice was steamed with steam and intermittently by a shower. It performed by the heating which adds water, and after performing the steaming process, the drying process was performed and the fermented germination brown rice from which gelatinization degree differs was produced. In this case, if the brown rice is kept steamed, the surface will eventually dry, and no matter how much steaming, the water will not penetrate into the rice and the brown rice will not gelatinize. Thus, by steaming with hot water and steaming with brown rice while steaming with steam, the surface is wet and gelatinized well, and a product with a relatively high gelatinization degree of 60 to 85% can be easily produced. Further, by adding water, it is not necessary to use saturated steam, that is, brown rice having a desired gelatinization degree can be produced without using a sealed steamer. In addition, the water content of each fermented germinated brown rice is 12 to 16%.
上記表5は、糊化度の異なる発酵発芽玄米を炊飯して食した結果を示し、10名のパネラーによるブラインドテストを行った。 Table 5 above shows the results of cooking and eating fermented germinated brown rice having a different degree of gelatinization, and a blind test was conducted by 10 panelists.
玄米ごはんと、本実施例の発酵発芽玄米ごはんとを比較し、「−3」〜「+3」の7段階評価を行った。尚、「0」は玄米ごはんと同じ、「−3」が最も悪い、「+3」が最も良い、の評価であり、「外観」「香り」「味」「粘り」「硬さ」についてそれぞれ評価し、数値の平均を総合評価とした。 The brown rice rice and the fermented germinated brown rice rice of this example were compared, and a seven-step evaluation of “−3” to “+3” was performed. “0” is the same as brown rice rice, “−3” is the worst, “+3” is the best, and “appearance”, “scent”, “taste”, “stickiness” and “hardness” are evaluated respectively. The average of the numerical values was taken as the overall evaluation.
上記表5のように、外観についての評価は大きな違いは見られなかったが、その他の「香り」「味」「粘り」「硬さ」については、いずれも高い評価が得られ、発酵発芽玄米において、糊化度を60〜85%とすることにより、従来にない高い食味評価を得ることができた。また、「硬さ」の評価で、本実施例の発酵発芽玄米が軟らかいて良い食感を与えることが分かる。 As shown in Table 5 above, no significant difference was observed in the appearance, but the other “scent”, “taste”, “stickiness”, and “hardness” were all highly evaluated, and fermented germinated brown rice , The pasty degree was set to 60 to 85%, and an unprecedented high taste evaluation could be obtained. Moreover, it is understood from the evaluation of “hardness” that the fermented germinated brown rice of this example is soft and gives a good texture.
尚、発酵発芽玄米となる玄米の種類を代えて実験を行ったが、玄米の種類により食味に大きな違いは見られなかった。このことから、発酵させることにより、玄米の種類によらず食味に優れた発酵発芽玄米ごはんが得られることが分かった。 In addition, although it experimented by changing the kind of brown rice used as fermented germination brown rice, the big difference was not looked at by the kind of brown rice. From this, it was found that fermented and germinated brown rice rice having excellent taste was obtained by fermenting regardless of the type of brown rice.
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