JP4585227B2 - Slimming kit - Google Patents

Description

      The present invention relates to a slimming kit in which an external preparation and an oral preparation are used in combination.

  It is well known that obesity and overweight, which have been increasing in recent years, cause not only a cause of various diseases such as diabetes but also a great social and economic burden. Even if it does not lead to obesity or overweight until it causes various diseases, it is difficult to say that it is a cosmetically desirable phenomenon. However, in order to reduce body fat, it is necessary to greatly change the current lifestyle, and for example, methods such as limiting the amount of food to be consumed and burning fat by various aerobic exercises have been taken. According to these methods, the effect of reducing body fat may be shown in a short period of time, but it may be accompanied by mental distress, it is not an effective means, and everyone has difficulty There is a need to develop agents and methods that can be effectively slimmed.

  As an effective slimming method, it is widely known to improve the accumulation of subcutaneous fat that affects the formation of body shape and the accumulation of bodily fluids generally called swelling (Non-patent Document 1). As an improvement means, slimming cosmetics such as massage gels and creams have been developed that promote the circulation of bodily fluids by reducing excess fat in the face and body or increasing metabolism in the body. In adipose tissue edema called cellulite, the skin surface shape collapses due to fat cell hypertrophy and body fluid retention, and irregularities and sagging occur on the skin surface of the buttocks and thighs. Therefore, it is also effective as a slimming method to activate the dermal tissue that contributes to the skin shape beam.

  Some slimming cosmetics are blended with a lipid degradation promoter that promotes lipid degradation of fat cells constituting fat for the purpose of providing a slimming effect. Substances that promote lipolysis include caffeine, eucommia, seaweed, coffee tree, chlorella, aonori, kombu, wakame, giant kelp, hijiki, hibamata, hijirimen, tengusa, tosakanori, giraffe, tsunomata, kotojitsunomata, cedar tree, cedar and plant And seaweed extracts are known (Patent Document 1).

  Further, seaweed, extract of Bakuryo, etc. are known as components that improve the retention of body fluid appearing in the swelling (Non-patent Document 1). In addition, Maronnier is a plant that has been used for a long time for the treatment of inflammation and swelling (Non-patent Document 2), and it has become clear that it has the effect of promoting lipolysis when used in combination with other plant extracts. (Patent Documents 1 and 2).

  Skin exposed to pressure due to fat cell swelling and swelling maintains its smooth shape by extracellular matrix components such as collagen and elastin in the dermis layer. Therefore, it is important for the maintenance of skin tension to proliferate and activate fibroblasts that produce these matrix components. Chlorella, aloe vera, rice, moon peach, hibiscus extracts and the like have been reported as skin fibroblast growth promoting components (Patent Documents 3 to 6).

  On the other hand, for foods, effective materials are being developed from the viewpoints of fat absorption inhibition, appetite suppression, fat accumulation inhibition, metabolism promotion, and fluid circulation improvement. There are preparations having such effects as pharmaceuticals, but daily food use is prohibited by the Food Sanitation Law. Therefore, natural substances that can be used as foods to replace these pharmaceuticals are desired.

  So far, Gymnema sylvestre which suppresses sugar absorption, chitosan which inhibits lipid absorption, Garcinia Cambodia which suppresses fat synthesis, etc. are known, and diet supplements using these are also known (patents) Reference 7).

  In addition, as a food that improves body fluid circulation, that is, eliminates swelling, products that are supplemented with vitamin E, which has a high function of promoting blood circulation, or that contain plants containing branched chain amino acids, coumarins or their derivatives have been developed. (Patent Document 8).

However, nothing is mentioned that a particular slimming effect can be obtained by selecting a specific component according to the application method, and simultaneously taking and applying it from the oral and dermal routes.
Usefulness of cosmetics-Progress and future prospects of evaluation technology, 2001, Yakuji Nipposha Functional research of medicinal herbs, 1999, Health Industry Newspaper Patent No. 3091782 Japanese Patent No. 2982872 JP 10-36283 A JP 2002-128632 A JP2002-3390 Japanese Patent Laid-Open No. 9-295928 JP 2001-321126 A Japanese Patent No. 3310649

  The present invention uses, in combination, an external preparation containing at least one of components having at least a lipolysis enhancing action and / or a fibroblast proliferation promoting action and an oral preparation having a lipolysis and fluid circulation promoting action. Thus, an object of the present invention is to provide a kit for use in a method for achieving a safer slimming effect by promoting the degradation of fat cells from the inside and outside of the body and improving the circulation of body fluids.

  The present inventor uses an external preparation containing at least one of components having at least a lipolysis enhancing action and / or a fibroblast proliferation promoting action in combination with an oral preparation having a lipolysis and fluid circulation promoting action. As a result, it was found that an excellent slimming effect was obtained, and the present invention was completed.

That is, the present invention
An external preparation , an external preparation and a body fluid, which are used in a cosmetic method for slimming, and contain at least one or more of components having a lipolysis enhancing action and / or a fibroblast proliferation promoting action A slimming kit comprising an oral preparation having an effect of improving circulation, promoting lipolysis and suppressing adipocyte increase ,
1. External preparation for slimming containing chrysanthelum indicum extract and maronier extract,
2. Weight loss characterized by including an external preparation containing Chrysanthelum indicum extract, Maronnier extract and Tissou extract, and an oral preparation containing Shrimp Shrimp, Butcher's Bloom, Rosemary, Caffeine and Catechin For slimming and body fat reduction ,
About.

  By carrying out the present invention, an external preparation containing one or more of the components having a lipolysis enhancing action and / or fibroblast proliferation promoting action, and improving fluid circulation, promoting lipolysis and inhibiting adipocyte proliferation A kit comprising an oral agent is provided. By using this kit, it acts from inside and outside the body, promotes the degradation of fat cells and improves the circulation of body fluids, and can achieve a higher slimming effect safely.

  In the external preparation of the present invention, a chrysanthelum indicum extract and a maronier extract are blended as components having a lipolysis enhancing action.

  Chrysanthellum indicum originates from South America such as Peru and Bolivia and has golden petals, so it is also known as the golden chamomile. Traditionally used as a remedy for liver, bile, and heart disease, as well as for wounds caused by inflammation and sedation, and has recently been shown to have anticalculus and antioxidant activities. Yes.

  Horse chestnut (Aesculus Hippocasstanum) is a plant belonging to the family Acecaceae and is also called horse chestnut. The part used as an extract is a seed, and escin contained in the seed is used as a therapeutic agent for inflammation and swelling.

In addition, as a component having a fibroblast proliferation promoting action, it is possible to use Zizyphus jujuba Miller
var.inermis Rehder). Taisou is a plant belonging to the family Aceraceae, also called a jujube. Roots, bark, leaves, fruit nuclei, etc. are used for various purposes as traditional Chinese medicines.

  Further, the topical preparation of the present invention includes Centella asiatica, Arnica Montana, Akibia quinata Decaisne or Akebia trifoliata Koidzumi, Alfalfa (Medicago sativa), Kudu (Puerariae Radix; Pueraria lobata Ohwi), Ezoukogi You may mix | blend the 1 type, or 2 or more types of plant extract chosen from (Eleuthero Ginseng; Acanthopanax Senticosus).

In the external preparation of the present invention, the compounding ratio of the above-mentioned components is not particularly limited, and may be appropriately changed according to the degree of expression of the intended improvement effect, the constitution of the user, the specific components used, and the like. However, typically, Chrysantherm indicum extract should be blended in a ratio of 0.0001 to 10%, Maronier extract in a ratio of 0.0001 to 10%, and Isolate extract in a ratio of 0.0001 to 10%. Is preferred. If these blending agents are less than 0.0001%, the intended effect may not be exhibited. If the blending agent exceeds 10%, the plant to be blended is denatured in a sunlight environment, and the effect is reduced. In addition, an unpleasant odor may be generated. Furthermore, it may cause skin irritation, which is not preferable from the viewpoint of safety to the skin.

  The external preparation of the present invention can be used as cosmetics, quasi drugs, pharmaceuticals, for example, liquid solutions such as aqueous solutions, oil agents, emulsions, suspensions, semi-solid agents such as gels and creams, powders, solids, etc. It can be applied in the form of a solid agent. It can be prepared in these forms by a conventionally known method, and can be made into various dosage forms such as lotions, emulsions, gels, creams, ointments, plasters, haps, aerosols and the like.

  The external preparation of the present invention includes fats and oils such as vegetable oils, higher fatty acids, higher alcohols, silicones, anionic surfactants, cationic surfactants, amphoteric surfactants, nonionic surfactants, preservatives, sugars, metals A sequestering agent, a polymer such as a water-soluble polymer, a thickener, a powder component, a UV absorber, a UV blocker, a moisturizer such as hyaluronic acid, a fragrance, a pH adjuster, and the like can be contained. . Vitamins, resident bacteria control agents, active oxygen scavengers, anti-inflammatory agents, other medicinal components such as bactericides, and physiologically active components can also be included.

  Examples of oils and fats used as additive components include liquid oils such as camellia oil, evening primrose oil, macadamia nut oil, olive oil, rapeseed oil, corn oil, sesame oil, jojoba oil, germ oil, wheat germ oil, glyceryl trioctanoate, etc. , Cacao butter, coconut oil, hardened coconut oil, palm oil, palm kernel oil, owl, owl kernel oil, hardened oil, hardened castor oil and other solid fats, beeswax, candelilla wax, cotton wax, nuta wax, lanolin, lanolin acetate And waxes such as liquid lanolin and sugarcane wax.

  Examples of the hydrocarbons include liquid paraffin, squalene, squalane, and microcrystalline wax.

  Examples of higher fatty acids include lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and the like.

  Examples of the higher alcohol include linear alcohols such as lauryl alcohol, stearyl alcohol, cetyl alcohol, and cetostearyl alcohol, and branched chain alcohols such as monostearyl glycerol ether, lanolin alcohol, cholesterol, phytosterol, and octyldodecanol.

  Examples of silicone include linear polysiloxanes such as dimethylpolysiloxane and methylphenylpolysiloxane, and cyclic polysiloxanes such as decamethylcyclopentasiloxane.

  Examples of the anionic surfactant include fatty acid salts such as sodium laurate, higher alkyl sulfates such as sodium lauryl sulfate, alkyl ether sulfates such as POE lauryl sulfate triethanolamine, N-acyl sarcosine acid, sulfosuccinate , N-acyl amino acid salts and the like.

  Examples of the cationic surfactant include alkyltrimethylammonium salts such as stearyltrimethylammonium chloride, benzalkonium chloride, and benzethonium chloride.

  Examples of amphoteric surfactants include betaine surfactants such as alkyl betaines and amide betaines.

  Examples of nonionic surfactants include sorbitan fatty acid esters such as sorbitan monooleate and hydrogenated castor oil derivatives.

  Examples of preservatives include methyl paraben and ethyl paraben.

  Examples of the sequestering agent include edetates such as disodium ethylenediaminetetraacetate, edetic acid, and sodium edetate.

  Examples of polymers include gum arabic, gum tragacanth, galactan, guar gum, carrageenan, pectin, agar, quince seed, dextran, pullulan, carboxymethyl starch, collagen, casein, gelatin, methylcellulose, methylhydroxypropylcellulose, hydroxyethylcellulose, carboxymethylcellulose Examples thereof include vinyl polymers such as sodium (CMC), sodium alginate, and carboxyvinyl polymer (such as CARBOPOL).

  Examples of the thickener include carrageenan, tragacanth gum, quince seed, casein, dextrin, gelatin, CMC, hydroxyethyl cellulose, hydroxypropyl cellulose, guar gum, xanthan gum, bentonite and the like.

  Examples of the powder component include talc, kaolin, mica, silica, zeolite, polyethylene powder, polystyrene powder, cellulose powder, inorganic white pigment, inorganic red pigment, titanium oxide coated mica, titanium oxide coated talc, and colored titanium oxide coated mica. And organic pigments such as Red No. 201 and Red No. 202.

  Examples of the ultraviolet absorber include paraaminobenzoic acid, phenyl salicylate, isopropyl paramethoxycinnamate, octyl paramethoxycinnamate, 2,4-dihydroxybenzophenone, and the like.

  Examples of the ultraviolet blocking agent include titanium oxide, talc, carmine, bentonite, kaolin, and zinc oxide.

  Examples of humectants include polyethylene glycol, propylene glycol, dipropylene glycol, 1,3-butylene glycol, 1,2-pentanediol, glycerin, diglycerin, polyglycerin, xylitol, maltitol, maltose, sorbitol, glucose, fructose. , Sodium chondroitin sulfate, sodium hyaluronate, sodium lactate, pyrrolidone carboxylic acid, cyclodextrin and the like.

Examples of the medicinal component include vitamin Cs such as L-ascorbic acid, esters thereof, L-ascorbic acid-2-glucoside, pantothenic acids such as calcium pantothenate, vitamin Ds such as vitamin D ₂ and cholecalciferol And the like vitamins.

  Whitening agents such as placenta extract and glutathione, skin activators such as royal jelly and beech extract, glycyrrhizic acid derivatives, glycyrrhetinic acid derivatives, anti-inflammatory agents such as azulene, amino acids such as arginine, serine, leucine and tryptophan, control agents for resident bacteria And maltose sucrose condensate, lysozyme chloride and the like.

  In addition, chamomile extract, parsley extract, wine yeast extract, grapefruit extract, honeysuckle extract, rice extract, grape extract, loquat extract, duckweed extract, assembly extract, merilot extract, birch extract, licorice extract, loach extract, loofah extract, capsicum extract, Examples include various extracts such as lemon extract, gentian extract, perilla extract, rosemary extract, sage extract, thyme extract, tea extract, seaweed extract, cucumber extract, clove extract, and hamamelis extract.

  In the oral preparation of the present invention, ingredients having an action of improving body fluid circulation, promoting lipolysis and inhibiting adipocyte proliferation are added.

In the implementation of the oral preparation of the present invention, as a component having preferable body fluid circulation improvement, lipolysis promotion, and adipocyte proliferation inhibitory action, a shrimp (Melilotus) which is a plant containing coumarins or derivatives thereof is used.
officinalis), marronnier, bursas (Asperula)
odorata), and one or more selected from Henruda, butchers bloom (Ruscus aculeatus), rosemary (Rosmarinas Officinalis), caffeine, catechin.

  Shrimp shrimp, horse chestnut, walnut, henruda, butcher's bloom, and rosemary use plants or extracts thereof. In addition, caffeine and catechin are separated from synthetic or natural products by known methods, respectively. When obtaining the above plant extract and caffeine, catechin from natural products, dry the flowers, seeds, pulp, roots, etc. of the plants and use them as powder, or dry, pulverize them, water, ethanol, etc. As the extract extracted at room temperature, warming, and overheating, a solution obtained by concentrating the extract into a powder can be used.

  In the oral preparation of the present invention, the intake amount of the above-mentioned components is not particularly limited, and may be appropriately changed according to the degree of the intended improvement effect, the constitution of the user, the specific components used, and the like. I can do it. For example, a plant containing coumarins or derivatives thereof, such as shrimp shrimp, horse chestnut, prawn or its extract, butcher's bloom or its extract, rosemary or its extract, has a dry weight of 10,000 to 0.001 mg / The daily intake of caffeine and catechin is preferably 10,000 to 0.001 mg / day.

  In both external preparations and oral preparations, commercially available plant extracts can be used. However, each plant is treated with water or ethanol, 1,3-butylene glycol, 1,2-pentanediol, propylene glycol, etc. What was extracted using a simple organic solvent can also be used. In the extraction, it is more efficient to carry out the extraction of the fruits, seeds, leaves, stems, roots, etc. as they are, or after processing such as chopping, drying, crushing, etc. according to the plant as the raw material. Extraction can be performed by immersing in an extraction solvent, stirring, or homogenizing or pressurizing in the extraction solvent. The extraction temperature is suitably about 5 to 100 ° C., and the extraction time can be appropriately set between about 5 minutes and 10 days.

  Especially for plants used for oral preparations, all parts such as leaves, stems, buds, flowers, woody parts, bark parts (bark), ground parts such as roots, root nodules, seeds, fruits and resins can be used. It is. Those parts of the plant are dried products that have dried themselves, pulverized products thereof, juices obtained by squeezing themselves, crude extracts with water or organic solvents such as alcohol, ether, acetone, and This includes all the fractions of the extract obtained by stepwise purification by various chromatographies such as partitioning and column chromatography. These may be used alone or in combination of two or more.

  As an oral preparation, it can be applied as a food such as a pharmaceutical or dietary supplement, a functional food, a health food, a food for specified health use, a food for slimming. Examples of the form include solid preparations such as powders, powders, granules, tablets and capsules, liquid preparations such as solutions, suspensions and emulsions, freeze-dried preparations and the like, and these preparations are prepared by conventional means. It is possible. If necessary, additives such as a stabilizer, a wetting agent, an emulsifier, a binder, a tonicity agent, a thickener, and a coating agent such as corn starch or shellac can be appropriately added. Examples thereof include glucose, lactose, sucrose, starch, mannitol, dextrin, fatty acid glyceride, propylene glycol, glycerin fatty acid ester, sucrose fatty acid ester, amino acid, gelatin, albumin, water, and physiological saline. Moreover, it can also mix | blend with drinks or confectionery like juice as a foodstuff.

  Other fat-soluble vitamins, water-soluble vitamins and foods and additives used in foods can also be blended.

  As a formulation technique, it can be manufactured according to a formulation method generally used as a health food, an oral preparation in medicine, a cosmetic, or an external preparation for pharmaceuticals.

By the present invention, it is possible to provide an excellent external preparation for slimming by combining specific ingredients, and slimming that exhibits a further excellent slimming effect by using a combination of the food for external application and specific ingredients Kits can be provided.
Hereinafter, the present invention will be described in detail.

[Test Example 1: Enhancement of lipolytic activity of components used in external preparations]
An adipocyte culture medium (F-PM-1, Dainippon Pharmaceutical) supplemented with Chrysantherm indicum extract and Maronnier extract to normal human white adipocytes (Dainippon Pharmaceutical) whose lipolysis was induced by adrenaline. The effect of enhancing lipolytic activity was evaluated by a method of applying and measuring the amount of fatty acid released.

To white adipocytes seeded in a 96-well multiwell plate, Adrenaline 10 ⁻⁶ M that triggers lipolysis and Formulation Examples 1 to 4 in Table 1 in which each extract was adjusted in concentration were applied at 37 ° C., 5 ° C. 5 hours and cultured in% CO ² under saturated steam. After the cultivation, the amount of free fatty acid in the cell supernatant was measured using a free fatty acid amount measurement kit (NEFAC-Test Wako; manufactured by Wako Pure Chemical Industries, Ltd.).

  From the number of fat cells per well and the amount of free fatty acid, the amount of free fatty acid per differentiated cell was calculated. Furthermore, the following formula 1 was used, and the free fatty acid amount in the blending example 1 as a control group was taken as 100%, and the fatty acid liberation rate in the blending examples 2 to 4 as the respective extract addition sections was calculated. The results are shown in Table 1.

Formula 1
[Fatty acid release rate (%)] = [Amount of free fatty acid per differentiated cell in each extract addition group (Eq / cell)] / [Amount of free fatty acid per differentiated cell in the control group (Formulation Example 1) (Eq / cell) × 100

  As shown in Formulation Examples 2 and 3, when 0.1% Chrysantherm indicum extract and Maronnier extract were added alone, fatty acid release was inhibited and no lipolytic activity enhancing effect was observed. It was. However, in Formulation Example 4, which is a composition of the present invention in which these two extracts are combined, the fatty acid release rate was promoted to about 5.9 times that of the control group.

[Test Example 2: Fibroblast proliferation promotion test of components used for external preparation]
MTT (3- (4,5-dimethy), a test method that can quantify the number of living cells
l-2-triazolyl) -2,5-diphenyl-2H-tetrazolium
Using bromide reduction method, cell proliferation promotion evaluation test of human-derived normal skin fibroblasts was performed.

96-well plate 1 was prepared by suspending human-derived normal skin fibroblasts (manufactured by Clonetics) in DMEM medium (Dulbecco's modified Eagle MEM medium, Nissui Pharmaceutical) containing 10% FBS (fetal bovine serum; manufactured by Invitrogen). The cells were seeded at 5 × 10 ³ cells per well and cultured at 37 ° C. under 5% CO ₂ % saturated steam for 24 hours.

After culturing for 24 hours, the cells fixed in the wells were washed with PBS (phosphate buffered saline, manufactured by Nissui Pharmaceutical Co., Ltd.), and for Formulation Examples 6 to 8 which are the constitution of the present invention, the concentration of the peanut extract was added. The mixture was replaced with a DMEM medium containing 5% FBS, and for Formulation Example 5 as a control group, only the DMEM medium containing 5% FBS was applied, and both were cultured at 37 ° C. and 5% CO _{2 for an} additional 120 hours.

After completion of the culture, 200 μL of MTT (manufactured by Dojindo Laboratories) solution adjusted to 0.3 mg / ml in 10% FBS-containing DMEM medium was applied to each well and cultured at 37 ° C., 5% CO _{2 for} 2 hours. Went. After completion of the reaction culture, the absorbance of the culture supernatant in each well at a measurement wavelength of 540 nm and a control wavelength of 630 nm was measured using a microplate reader. Using formula 2, the cell growth rate in Formulation Examples 6 to 8 was calculated from the measured absorbance. The results are shown in Table 2.

Formula 2. Cell growth rate calculation formula [cell growth rate (%)] = ([Example absorbance (540 nm)] − [Example absorbance (630 nm)]) / ([Control absorbance (540 nm)] − [Control absorbance (630 nm) )]) X 100

  In Formulation Examples 6 to 8 which are the constitution of the present invention to which an extract of Taisou was added, cell growth was promoted in a concentration-dependent manner, and the effect of promoting the proliferation of fibroblasts by the extract of Tiso was shown.

  From the results of Test Examples 1 and 2, it was confirmed that the agent containing the Chrysantherm indicum extract, the Marronnier extract, and the Taiso extract had the lipolysis enhancing action. An external preparation containing these components promotes fat metabolism and exerts a slimming effect by promoting fibroblast proliferation.

External preparations (gel agents) and oral preparations used in the kit of the present invention were produced according to conventional methods according to the following formulation examples. The unit is mass%.

[Prescription Example 1] Gel water 89.0
Carbomer K 0.2
Glycerin 10.0
Chrysanthelum indicumum extract 0.5
Maronier extract 0.2
Taisou extract 0.1

[Prescription Example 2] Soft Capsule Shrimp Extract 8.0
Butcher's Bloom extract powder 8.0
Rosemary extract 8.0
Caffeine 8.0
Catechin 16.0
Rice germ oil 48.0
Beeslow 4.0

[Test Example 3: Slimming kit monitor test]
Forty women were divided into two groups of only the A external preparation and the oral preparation use group B external use use group, and a continuous test for 3 months was conducted. As an external preparation, the gel of Formulation Example 1 was used once a day in the abdomen and buttocks, and as an oral preparation, 6 capsules (250 mg) of Formulation Example 2 were consumed daily.

  Before monitoring, the waist circumference, hip circumference, body weight and body fat mass at the navel position were measured every month, 2 months and 3 months after the start of monitoring. An index of the slimming effect was obtained by subtracting the measured value before monitoring from the measured value after 3 months, as a change value. As a result of monitoring, a slimming effect was obtained by using an external preparation, and an even higher slimming effect was obtained by using in combination with an oral preparation. The results are shown in Table 3.

The oral preparation used in the kit of the present invention was produced according to a conventional method according to the following formulation example. The unit is mass%.

[Prescription Example 3] Tablet Shrimp Extract 6
Butchers Bloom Extract 10
Rosemary extract 10
Caffeine 5
Catechin 18
Cellulose 48
Sucrose fatty acid ester 3

[Test Example 4: Slimming kit monitor test]
The test was performed using the external preparation of Formulation Example 1 and the oral preparation of Formulation Example 3.
(Target)
Healthy women who are interested in dieting (exclusion criteria)
Those who fall under the following items were excluded from the subjects in this study.
Those who are taking treatment during pregnancy or breastfeeding, or who are undergoing treatment such as during a hospital visit (test item)
External preparation: Formulation Example 1 It was applied daily to the buttocks and legs.
Oral preparation: Formulation Example 3 Four tablets (one tablet 250 mg) were ingested daily.

(Test method)
The test period was 8 weeks, and all the subjects were in an open test of a group using only the external preparation or a group using both the external preparation and the oral preparation. Hereinafter, they are referred to as a lotion group and a combination group, respectively.

(Survey item)
The survey items were body part circumference measurement, body weight measurement, and body fat percentage measurement.
The subject took all measurements after changing into a predetermined measurement garment. The circumference of the body part was measured by measuring the circumference of the hip (the thickest position), thigh, and calf. The hip was measured from above the measurement garment, while the other parts were measured on the exposed part of the skin. The body fat percentage was measured by a measuring device Inbody using a multi-frequency impedance method.
The measurement data was expressed as an average value ± standard error. The test was performed at each time point within the group by “corresponding t test”, and the significance level was 5% or less on both sides. All analyzes used EXCEL.

(Subject background)
The test subjects were 33 women, and 17 lotion groups and 16 combination groups were assigned so that there was no difference between groups in terms of body fat percentage and age. Mean values ± SD are shown in Table 4.

(Implementation period)
The implementation period is as follows. After the first measurement, an external preparation, or an external preparation and an oral preparation were handed over, and intake was started from the next day.
First measurement (before use)
Second measurement (after 4 weeks)
Third measurement (after 8 weeks)
Measurements were taken after 15:00. Since one of the subjects had difficulty adjusting the date and time, measurements were always taken in the morning. For the other two, only the final measurement was taken in the morning.

(Measurement result)
(1) In both hip circumference groups, there was a slight increase tendency after 4 weeks, and after 8 weeks, there was a significant decrease in the combination group (α = 0.046) and the lotion group (α = 0.007) (Table 5).
Also in the rate of change, a significant decrease was observed after 8 weeks in both groups (combination group α = 0.006, lotion group α = 0.038), and the decrease in the combination group was particularly remarkable (Table 6).

Hip circumference change

Hip circumference change rate

(2) In the thigh surrounding combination group, it decreased significantly after 4 weeks (α = 0.004) and after 8 weeks (α = 0.0004). In the lotion group, there was a significant decrease (α = 0.002) after 8 weeks (Table 7).
A significant decrease (α = 0.002) was observed after 8 weeks in the lotion group, and a significant decrease was observed after 4 weeks (α = 0.004) and 8 weeks (α = 0.0003) in the combination group. In particular, the decrease in the combination group was rapid and remarkable (Table 8).

Thigh circumference change

Thigh circumference change rate

(3) Both groups around the calves decreased significantly after 8 weeks. (Combination group α = 0.0002, lotion group α = 0.0045) (Table 9).
There was also a significant decrease in the rate of change after 8 weeks in both groups. (Combination group α = 0.0003, lotion group α = 0.0005) (Table 10).

Calf circumference change

Calf circumference change rate

(4) The body weight combination group showed a decreasing trend after 4 weeks and a significant decrease after 8 weeks (α = 0.007). Although a decreasing trend was seen in the lotion group, it was not significant (Table 11).
Regarding the rate of change, a significant decrease (α = 0.012) was observed after 8 weeks in the combination group. There was little change in the lotion group (Table 12).

Weight change

Weight change rate

(5) In the body fat percentage combination group, a decreasing tendency was observed after 4 weeks, and a significant decrease (α = 1.5 × 10 ⁻⁶ ) was observed after 8 weeks. In the lotion group, there was a significant increase after 4 weeks (α = 0.033) and a significant decrease after 8 weeks (α = 0.0004) (Table 13).
In the lotion group, the change rate was significantly increased after 4 weeks (α = 0.028) and decreased significantly after 8 weeks (α = 0.0005). In the combination group, a significant decrease (α = 7.7 × 10 ⁻⁶ ) was observed after 8 weeks. After 8 weeks, the decrease was particularly remarkable in the combination group (Table 14).

Change in body fat percentage

Change rate of body fat percentage

The topical preparation used in this study was found to be effective in reducing the circumference of the lower body that women particularly want to lose. In addition, the combined use of an external preparation and an oral preparation markedly increased the reduction rate around the body.
The rate of change in body fat percentage decreased significantly after 8 weeks in both groups. The combination group showed a decrease of about 3%, the lotion group showed a decrease of about 2%, and the reduction rate was particularly high in the combination group.

Table 15 summarizes the slimming effect of the external preparation and oral preparation used in this test.

Although other prescription examples in the external preparation are shown below, the external preparation in the present invention is not limited to this prescription example.
[Prescription Example 4] Cream The following prescription (unit:% by mass) shows a cream formulation as an example of an external preparation used in the kit of the present invention.
Water 67.3
Xanthan gum 0.1
Glycerin 5.0
BG 5.0
Squalane 14.0
Stearic acid 2.0
Polysorbate 60 1.5
Palm fatty acid sucrose 0.3
Sorbitan stearate 4.0
Chrysanthelum indicumum extract 0.5
Maronier extract 0.2
Taisou extract 0.1

Claims (2)

An external preparation for slimming containing Chrysanthelum indicumum extract and Maronnier extract. An agent for use in a cosmetic method for slimming, comprising an external preparation containing Chrysanthelum indicum extract, Maronnier extract and Tisoiso extract , Shrimp shrimp, Butcher's bloom, Rosemary, Caffeine and A slimming kit for weight loss and body fat reduction , comprising an oral preparation containing catechin .