JP4630102B2 - Hyaluronic acid production promoter and cosmetics containing the same - Google Patents

Description

The present invention relates to a cosmetic intended to improve the skin wrinkles and wrinkles by promoting hyaluronic acid production of keratinocytes.

In recent years, as the aging society progresses, the role required for cosmetics has been increasing in order to beautifully grow older. However, due to internal factors such as aging and external factors such as ultraviolet rays and active oxygen, the skin's inherent functions such as contractility, flexibility, and moisture retention decline, causing various problems. To do.

Wrinkles, one of these troubles, are caused by a decrease in the number of cells that produce the extracellular matrix of the dermis, aging of cell functions due to a decrease in division rate, decrease and degeneration of collagen fibers, decrease of subcutaneous adipose tissue, etc. Occurs due to the occurrence of skin relaxation and loss of elasticity.

Conventionally, skin aging has been dealt with by using a composition that supplements the skin with substances such as collagen and hyaluronic acid that are lost due to aging, and an indirect combination of protective substances to protect the skin from ultraviolet rays and active oxygen. Anti-aging agents were mainstream. However, these methods have not been satisfactory. Further, as an attempt to fundamentally improve aging, particularly wrinkles produced, retinoic acid (Japanese Patent Laid-Open No. 2005-15388), α-hydroxy acid represented by glycolic acid, etc. 301883), however, they require a high blending amount, have problems in safety such as causing inflammation accompanied by swelling, etc., and could not withstand long-term use.
JP-A-8-259420 Japanese Patent Laid-Open No. 9-301883 JP 2005-15388 A

The problem to be solved is to provide a cosmetic material that can fundamentally improve the aging of the skin, has no harmful effects on the skin, and can be used safely and is effective in preventing aging.

Therefore, as a result of intensive studies in view of the actual situation, the present inventors have found that the extract obtained from the seeds of the cucurbitaceae plant activates the hyaluronic acid producing ability of keratinocytes and increases the production of extracellular matrix, thereby reducing the skin. As a result, the present invention has been completed.

That is, this invention provides the skin external preparation effective for anti-aging characterized by including the extract obtained from the seed of the Cucurbitaceae plant.

The seeds of the Cucurbitaceae plant used in the present invention are not particularly limited, but pumpkin (Cucurbita moschata Duch. Var. Toonas Makino) and loofah (Luffa cylindrical Roem.) Seeds are preferred. The extraction method is also not particularly limited, and can usually be obtained by extracting with various solvents and purifying the extract (extract).

That is, the extract obtained from the seeds of Cucurbitaceae plants can use water, various polar organic solvents and mixtures thereof, and a polar solvent acidified with hydrochloric acid as an extraction solvent.

The blending amount of the extract obtained from the seeds of the Cucurbitaceae plant according to the present invention with respect to various cosmetics is not particularly limited because it can be varied according to the cosmetic embodiment, cosmetic usage form, and the like. In principle, an effective amount may be present, but generally 0.0001 to 100% by mass in terms of dry weight can be used in a cosmetic composition, preferably 0.01 to 10%. It is 0.1 mass%, More preferably, it is 0.1-5.0 mass%.

The application range of the cosmetic according to the present invention is not particularly limited. That is, the present invention can be applied to all cosmetics that can use each function and effect according to the function and effect of the active ingredient of the present invention.

For example, the active ingredient according to the present invention is blended in various cosmetic bases, and various basic cosmetics such as creams, emulsions, lotions, packs, facial cleansers, foundations, lipsticks, cheeks, white powders, etc. It can be applied to a wide range of cosmetics such as makeup, hair washing, hair nourishing agents, shampoos and rinses, and other cosmetics such as soaps, beauty nails and eau de cologne. Moreover, the embodiment of the various cosmetics can be applied in various forms such as lotion, emulsion, ointment, sol, gel, powder, spray, and solid.

EXAMPLES Hereinafter, although an Example demonstrates this invention in detail, this invention is not limited to these.

[Example 1] As a raw material for preparing an extract obtained from pumpkin seeds, 50 g of endosperm excluding the seed coat of pumpkin seeds was used. After adding 200 ml of ion-exchanged water to 50 g of the raw material and heating and extracting at 60 ° C. for 6 hours, the supernatant is centrifuged to remove relatively large suspended matter, and then filtered using a filter paper having a mesh diameter of 0.22 μm. Concentration under reduced pressure using a rotary evaporator gave about 0.5 g of pumpkin seed extract.

[Example 2] Hyaluronic acid production test using keratinocytes (1) Preparation of test solution The pumpkin seed extract was added to 1.0% (w / v)% in DULBECCO'S MODIFICATION OF EAGLE'S MEDIUM (EME) medium containing 5% fetal bovine serum. After dissolution, the solution was sterilized by filtration through a 0.2 μm membrane filter and appropriately diluted to obtain a test solution.
(2) Cell culture Keratinocytes were cultured in DULBECCO'S-EME medium supplemented with 5 (v / v)% fetal bovine serum. Cells adjusted to 5 × 10 ⁵ cells / mL in the above medium were inoculated into 0.1 mL each in a sterile plastic 96-well plate with an inner diameter of 6.5 mm and cultured for 48 hours.
(3) Quantification of hyaluronic acid in culture medium Hyaluronic acid was quantified using a hyaluronic acid measurement kit manufactured by Seikagaku Corporation. The medium was taken out from the cultured cells, and 50 μL was added to a microplate on which hyaluronic acid binding protein (HABP) was immobilized. Subsequently, 50 μL of biotin-labeled HABP solution was added to each well. After mixing for 1 minute using a plate mixer, the mixture was allowed to stand at 37 ° C. for 60 minutes. (1 hour reaction) After washing each well, 100 μL each of HRP-labeled streptavidin solution was added, and then allowed to stand at 37 ° C. for 60 minutes. (Secondary reaction) After washing of each well, 100 μL of enzyme substrate solution was added, and then light-shielded with aluminum foil or the like and allowed to stand at room temperature for 30 minutes. (Luminescence reaction) 100 μL of a reaction stop solution was added to each well. The absorbance of each well was measured with a plate reader.

Table 1 shows the hyaluronic acid producing ability in keratinocytes of retinoic acid and cucurbitaceae seed extract.

From the results shown in Table 1, it was found that the cucurbitaceae seed extract clearly promotes hyaluronic acid production although it does not reach retinoic acid, which is said to have an effect of promoting high hyaluronic acid production.

The formulation example of this invention is given to the following.
<Formulation example 1> lotion (component name) (mass%)
Pumpkin seed extract 0.01
Glycerin 5.0
Polyoxyethylene sorbitan monolaurate (20E.0) 1.5
Ethanol 10.0
Antiseptic / Antioxidant Appropriate amount of perfume Appropriate amount of purified water Remainder

<Formulation example 2> Cream (component name) (mass%)
Pumpkin seed extract 0.0001
Beeswax 2.0
Stearyl alcohol 5.0
Stearic acid 8.0
Squalane 10.0
Self-emulsifying glyceryl monostearate 3.0
Polyoxyethylene cetyl ether (20E.0) 1.0
Glycerin 5.0
Potassium hydroxide 0.3
Perfume Appropriate amount of preservative / antioxidant Appropriate amount of purified water

<Prescription Example 3> Emulsion (component name) (mass%)
Pumpkin seed extract 0.1
Squalane 8.0
Vaseline 2.0
Beeslow 0.5
Sorbitan sesquioleate 0.8
Polyoxyethylene oleyl ether (20E.0) 1.2
Carboxyvinyl polymer 0.2
Glycerin 1.5
Potassium hydroxide 0.1
Ethanol 7.0
Perfume Appropriate amount of preservative / antioxidant Appropriate amount of purified water

<Prescription Example 4> Packing agent (component name) (mass%)
Pumpkin seed extract 0.01
Vinyl acetate resin emulsion 15.0
Polyvinyl alcohol 10.0
Jojoba oil 3.0
Glycerin 5.0
Titanium oxide 8.0
Kaolin 7.0
Ethanol 5.0
Perfume Appropriate amount of preservative / antioxidant Appropriate amount of purified water

<Prescription Example 5> Ointment (component name) (mass%)
Pumpkin seed extract 0.0001
Tocopherol acetate 0.5
Octyl paradimethylaminobenzoate 4.0
Butylmethoxybenzoylmethane 4.0
Stearyl alcohol 18.0
Owl 20.0
Glycerin monostearate 0.3
Vaseline 33.0
Perfume Appropriate amount of preservative / antioxidant Appropriate amount of purified water

<Formulation example 6> Powder foundation (component name) (mass%)
Pumpkin seed extract 0.01
Talc 43.0
Kaolin 18.0
Mica 8.0
Zinc oxide 10.0
Titanium oxide 5.0
Coloring pigment Appropriate amount Magnesium stearate 6.0
Liquid paraffin 4.0
White petrolatum 1.0
Ceresin 1.0
Isopropyl myristate 1.5
Preservative / Antioxidant Appropriate amount Perfume Appropriate amount

When 20 healthy adult women were allowed to use the cream of Prescription Example 2 for 1 month, 18 people obtained a result that skin moisture, firmness and wrinkles were improved. In addition, no panelists felt any abnormality on the skin during use.

According to the present invention, since a high hyaluronic acid production promoting effect is seen in the seed extract of Cucurbitaceae seed extract, particularly pumpkin and loofah, it is effective to improve wrinkles with high safety by containing it. Providing cosmetics can be expected.

Claims (1)

Hyaluronic acid production promoter comprising loofah seed extract