JP4723738B2 - Deodorant - Google Patents

Description

【００２２】
実施例１ β−グルクロニダーゼ阻害活性
０．１ｍｏｌ／Ｌ酢酸ナトリウム緩衝液（ｐＨ５．０）０．４mL、０．０６％フェノールフタレイン−β−Ｄ−グルクロニドナトリウム０．４mL及び１０％に希釈した植物抽出物０．１mLを試験管に入れ、３７℃で予備加温する（Ｂｌａｎｋも同じ）。０．２％塩化ナトリウム溶液で２５０ｕｎｉｔｓ／mLに希釈したβ−グルクロニダーゼ（和光純薬工業株式会社，ウシ肝臓製）を０．１mL入れ（Ｂｌａｎｋには入れない）、反応を開始する。３７℃で正確に３０分間加温し、５％トリクロロ酢酸溶液０．１mLを加え反応を停止する（Ｂｌａｎｋにも入れる）。Ｂｌａｎｋに０．２％塩化ナトリウム溶液で２５０ｕｎｉｔｓ／mLに希釈したβ−グルクロニダーゼ（和光純薬工業株式会社，ウシ肝臓製）を０．１mL入れた後、０．２ｍｏｌ／ｌグリシン溶液（ｐＨ１０．４）を３mL加え発色させる。吸収セル１０ｍｍを用い、５４０ｎｍにおける吸光度をＢｌａｎｋを対照液として測定する。サンプル（１０％に希釈した植物抽出物）の代わりに蒸留水０．１ｍを入れた物をコントロールとし、コントロールの吸光度とサンプルの吸光度から、下記式に基づきサンプル（１％植物抽出物）のβ−グルクロニダーゼ活性阻害率を計算した。
【００２３】
β−グルクロニダーゼ活性阻害（％）＝（１−（サンプルの吸光度／コントロールの吸光度））×１００
【００２４】
また、０．１％植物抽出物及び０．０１％植物抽出物についても、それぞれ１％及び０．１％に希釈した植物抽出物をサンプルとし、１％植物抽出物と同様にβ−グルクロニダーゼ活性阻害率を測定した。結果を図２に示す。
【００２５】
実施例２ 臭い発生抑制効果
（１）β−グルクロニダーゼ処理による臭い発生の確認
滅菌処理した秤量ビンに、以下に示す方法により採取し、濾過滅菌した汗を５mL入れ、０．２％塩化ナトリウム溶液で９００ｕｎｉｔｓ／mLに希釈したβ−グルクロニダーゼ（和光純薬工業株式会社，ウシ肝臓製）を０．５mL入れ（対照は０．２％塩化ナトリウム溶液０．５mL入れた）３７℃で２４時間インキュベートし、官能評価をした。結果を図３に示す。
【００２６】
（汗の採取法）
一般男性２６人の脇の下を、蒸留水１．５mLを湿らせた脱脂綿で拭き取った。
脱脂綿を絞り、回収した物（５０mL）を０．４５μｍのフィルターで濾過した後、ＭＩＬＬＩＰＯＲＥのセントリプレップＹＭ−１０で濾過し、凍結乾燥をした。これに５０ｍＭクエン酸緩衝液（ｐＨ５．０）１０mLを入れ汗とする。
【００２７】
（２）臭い発生抑制効果
滅菌処理した秤量ビンに、以下に示す方法により採取し、濾過滅菌した汗を２mL、濾過滅菌した１％ハマメリスエキスを０．２mL入れ（対照は、蒸留水０．２mL入れ）、０．２％ 塩化ナトリウム溶液で９００ｕｎｉｔｓ／mLに希釈したβ−グルクロニダーゼ（和光純薬工業株式会社，ウシ肝臓製）を０．２mL入れ３７℃で２４時間インキュベートし、官能評価をした。結果を図４に示す。
【００２８】
（汗の採取法）
一般男性２５人の脇の下を、蒸留水１．５mLを湿らせた脱脂綿で拭き取った。脱脂綿を絞り、回収した物（５０mL）を０．４５μｍのフィルターで濾過した後、ＭＩＬＬＩＰＯＲＥのセントリプレップＹＭ−１０で濾過をし、凍結乾燥をした。これに５０ｍＭクエン酸緩衝液（ｐＨ５．０）１０mLを入れ汗とした。
【００２９】
【発明の効果】
本発明のデオドラント剤及びβ−グルクロニダーゼ阻害剤は、皮膚の常在菌を殺すことなく、不快な体臭の発生を持続的に抑制できることから、優れた消臭効果と高い安全性を有する化粧料、外用医薬品又は医薬部外品として有用である。
【図面の簡単な説明】
【図１】植物及び菌類抽出物の皮膚常在菌に対する影響を示す図である。
【図２】植物及び菌類抽出物のβ−グルクロニダーゼ活性阻害率を示す図である。
【図３】β−グルクロニダーゼによる汗臭の変化を示す図である。
【図４】β−グルクロニダーゼ阻害による汗臭生成抑制効果を示す図である。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a deodorant agent that suppresses the generation of unpleasant body odors in humans.
[0002]
[Prior art and problems to be solved by the invention]
Control technology for unpleasant human body odors includes sweat control technology using antiperspirant materials, control technology for the growth of germs causing unpleasant odors using antibacterial materials, and technology for deodorizing unpleasant body odors. And masking technology by fragrance.
[0003]
Antiperspirant technology has been devised to suppress sweating using zinc paraphenol sulfonate, citric acid, various aluminum and zirconium salts, which have a relatively strong astringent action, and antibacterial technology is the cause of creating substances that cause body odor A method has been devised to suppress the generation of odors using antibacterial agents such as trichloric acid, benzalkonium chloride, benzethonium chloride, chlorhexidine hydrochloride, and halocarban, which have the function of suppressing the growth of fungi (including skin resident bacteria). ing.
[0004]
In addition, as a deodorizing technique, a method for suppressing the generation of specific odors by changing lower fatty acids, which are one of the causes of unpleasant body odors, into metal salts with zinc white (zinc oxide), flavonoids and chlorophylls. As a masking technique, a method of masking a weak body odor with a perfume or a cologne has been devised.
[0005]
However, conventional antiperspirant technology, deodorant technology and masking technology are not sufficient in terms of sustainability of the effect, and antibacterial technology is effective by disinfecting skin resident bacteria in addition to the cause of unpleasant odor. There is a suggestion that the primary barrier function may be reduced.
[0006]
An object of this invention is to provide the deodorant agent which can suppress unpleasant body odor continuously without killing the resident bacteria of skin.
[0007]
[Means for Solving the Problems]
The present inventors focused on androste 16-enes, which are causative substances of volatile steroid odor, which is one of the causes of unpleasant body odors in humans. It has been found that the extract has an excellent β-glucuronidase inhibitory activity, can suppress the generation of unpleasant body odor derived from androste 16-enes without killing skin resident bacteria, and is useful as a deodorant agent.
[0008]
That is, the present invention is a deodorant comprising a plant and fungi or an extract thereof selected from the following: And a β-glucuronidase inhibitor are provided.
[0009]
DETAILED DESCRIPTION OF THE INVENTION
Ougon of the present invention is the root of the Labiatae Scutellaria baicalensis Georgi excluding the pericy, and Gobaishi is an insect parasite of the aphids of the aphids on the leaves of the Nurde Rhus javanica L. of the Anacardiaceae ( Insects are the roots of Lithospermum erythrorhizon Siebold et Zuccarini in Boraginaceae, peonies are the roots of Ranunculaceae, and the roots of Paeonia lactinora PALL. japonicus Hara stems and leaves, saxifragaceae armchairs Hydrandea serrata Seringe var. thunbergii Makino leaves and branch tips, clove is myrtaceae Syzygium aromaticum Merrill et Perry buds, legumes legumes (Leguminosae) Glycyrrhiza uralensis Fischer, Glycyrrhiza glabra L. or other genus plants Ron, assembly is the whole plant of the flowering stage of assembly Swertia japonica Makino of gentianaceae (Gentianaceae), citrus unshiu peel means Rutaceae (Rutaceae) of satsuma mandarin Citrus unshiu Markovich, or other closely related plants mature pericarp, respectively.
[0010]
Gardenia jasminoides Elli (Rubiaceae) is mainly used for its flowers and fruits, and chamomile is Matricaria chamomilla L. (Compositae), mainly for its flowering petals. Is Umblliferae's Centella asiatica (L.) RUBAN, mainly using its whole plant, Comfrey is Boraginaceae's spinach Symphytum officinale L., mainly using its leaves and roots The nettle is the nettle (U. thumbergiana) of the Urtiaceae family, and its whole grass is mainly used, and the Hamelis is the American seaweed Hamamelis virginiana, mainly using its leaves and bark.
Cordyceps is a mushroom infested with insects of the Clavicipitaceae family.
[0011]
Such a plant or an extract thereof has β-glucuronidase inhibitory activity, and suppresses the production of androste 16-enes on the skin (Example 1 described later). Here, Androste 16-enes are volatile steroids that are the cause of human body odor, mainly glandular odor, and are secreted into sweat by glucuronic acid inclusion and increase in solubility, and inhabit the skin. Inhibiting β-glucuronidase, which is involved in the metabolism of glucuronic acid-encapsulated precursors, suppresses the production of androste 16-enes. Since it can suppress the generation of unpleasant body odor, it is useful as a deodorant agent.
Further, as the deodorant agent, those that do not exhibit antibacterial activity, desirably bactericidal properties against the skin resident bacteria involved in maintaining the barrier function of the skin are preferable, but also in this respect, the plant and fungal extracts are It is highly useful without killing skin resident bacteria (FIG. 1).
The term "not showing bactericidal properties" as used herein means that a resident bacterium is inoculated into an SCD medium containing 0.5% Tween 80, to which a test substance is added, and after reacting at 37 ° C for 24 hours, (Bacillus subtilis) means that the number of bacteria does not decrease to 1/10 or less of the control, and normal bacteria (Staphylococcus epidermidis) do not decrease to 1/100 or less of the control.
[0012]
The plant and fungi which are the active ingredients of the deodorant agent and β-glucuronidase inhibitor of the present invention are the whole plant or leaves, roots, rhizomes, fruits, seeds, flowers and fungi of the plant as it is or ground. And use. Further, in the present invention, the extract is further extracted at room temperature or under heating, or extracted by using an extractor such as a Soxhlet extractor, a dilute solution thereof, It means a concentrated liquid or its dry powder. Here, the extract may be a mixture obtained from two or more kinds of plants.
[0013]
As a solvent used for extraction, water; alcohols such as methanol, ethanol, propanol and butanol; polyhydric alcohols such as propylene glycol and butylene glycol; ketones such as acetone and methyl ethyl ketone; esters such as methyl acetate and ethyl acetate; tetrahydrofuran Linear and cyclic ethers such as diethyl ether; hydrogen halides such as dichloromethane, chloroform and carbon tetrachloride; hydrocarbons such as hexane, cyclohexane and petroleum ether; aromatic hydrocarbons such as benzene and toluene; polyethylene Polyethers such as glycol; pyridines and the like can be mentioned, and these can be used alone or as a mixture.
[0014]
In addition, it is possible to remove inactive contaminants from the extract by a technique such as liquid-liquid distribution, and it is preferable to use such an extract in the present invention. These may be used after performing treatments such as deodorization and decolorization by a known method if necessary.
[0015]
The plant and fungi or the extract thereof can be used as they are as the deodorant agent and β-glucuronidase inhibitor of the present invention, but after the extract is diluted, concentrated or freeze-dried, it is made into a powder or paste. It can also be prepared and used.
[0016]
The deodorant and β-glucuronidase inhibitor of the present invention can be used as a preparation such as a cosmetic, an external medicine or a quasi-drug, such as a cream, emulsion, lotion, powder, spray, stick, etc. It is also possible to use the methods in combination.
[0017]
When used as a cosmetic, topical medicine or quasi-drug, the content of deodorant or β-glucuronidase inhibitor is generally preferably 0.1 to 20% by weight, particularly 0.5 to 10% by weight. % Is preferable. Further, the content as a plant extract is generally preferably 0.0001 to 10% by weight, particularly preferably 0.001 to 5% by weight in terms of solid content.
[0018]
These cosmetics, external medicines or quasi drugs include various commonly used components such as oils, surfactants, alcohols, chelating agents, pH adjusters, preservatives commonly used as cosmetic ingredients. , UV absorbers, whitening agents, wrinkle improvers, moisturizers, sebum secretion inhibitors, softeners, keratin protective agents, medicinal agents, antioxidants, solvents, etc. Can be formulated in any combination.
[0019]
The deodorant agent and β-glucuronidase inhibitor of the present invention can control the generation of an unpleasant odor by applying them locally to a place where an unpleasant odor is likely to occur, such as a foot, a heel, a head, or a genital region. In such a case, the amount of the preparation to be used varies depending on the content of the active ingredient. For example, in the case of a liquid preparation, 1-20 mg per 1 cm ^{2 of the} skin surface, and in the case of a solid preparation, it is preferably 1-50 mg.
[0020]
【Example】
As the plant and fungal extracts, the following commercial products conforming to the cosmetic raw material standards were used.
[0021]
[Table 1]

[0022]
Example 1 β-glucuronidase inhibitory activity 0.1 mol / L sodium acetate buffer (pH 5.0) 0.4 mL, 0.06% phenolphthalein-β-D-glucuronide sodium 0.4 mL and a plant diluted to 10% Place 0.1 mL of extract in a test tube and pre-warm at 37 ° C. (same for Blank). 0.1 mL of β-glucuronidase (Wako Pure Chemical Industries, Ltd., manufactured by Bovine Liver) diluted to 250 units / mL with 0.2% sodium chloride solution is added (not added to Blank) to start the reaction. Warm exactly 30 minutes at 37 ° C., and stop the reaction by adding 0.1 mL of 5% trichloroacetic acid solution (also put into Blank). After adding 0.1 mL of β-glucuronidase (Wako Pure Chemical Industries, Ltd., manufactured by Bovine Liver) diluted to 250 units / mL with 0.2% sodium chloride solution in Blank, 0.2 mol / l glycine solution (pH 10.4) Add 3 mL) to develop color. Using an absorption cell of 10 mm, the absorbance at 540 nm is measured using Blank as a control solution. Instead of the sample (plant extract diluted to 10%), the one containing 0.1m of distilled water was used as a control. From the absorbance of the control and the absorbance of the sample, the β of the sample (1% plant extract) was calculated based on the following formula. -The glucuronidase activity inhibition rate was calculated.
[0023]
β-glucuronidase activity inhibition (%) = (1− (absorbance of sample / absorbance of control)) × 100
[0024]
In addition, with respect to 0.1% plant extract and 0.01% plant extract, the plant extract diluted to 1% and 0.1%, respectively, was used as a sample, and β-glucuronidase activity was obtained as in the case of 1% plant extract The inhibition rate was measured. The results are shown in FIG.
[0025]
Example 2 Odor Occurrence Suppressing Effect (1) Confirmation of Odor Occurrence by β-Glucuronidase Treatment Into a sterilized weighing bottle, 5 mL of sweat sterilized by filtration and sterilized by filtration is placed in a 0.2% sodium chloride solution. 0.5 mL of β-glucuronidase (Wako Pure Chemical Industries, manufactured by Bovine Liver) diluted to 900 units / mL was added (control was 0.5 mL of 0.2% sodium chloride solution) and incubated at 37 ° C. for 24 hours. Sensory evaluation was performed. The results are shown in FIG.
[0026]
(How to collect sweat)
The armpits of 26 ordinary men were wiped with absorbent cotton moistened with 1.5 mL of distilled water.
Absorbent cotton was squeezed, and the collected product (50 mL) was filtered through a 0.45 μm filter, then filtered through MILLIPORE Centriprep YM-10, and lyophilized. 10 mL of 50 mM citrate buffer (pH 5.0) is added to this to make sweat.
[0027]
(2) Odor generation suppression effect In a sterilized weighing bottle, 2 mL of sweat sterilized by filtration and 0.2 mL of 1% Hamamelis extract sterilized by filtration are added (control is 0.2 mL of distilled water). Put), 0.2 mL of β-glucuronidase (Wako Pure Chemical Industries, Ltd., manufactured by Bovine Liver) diluted to 900 units / mL with 0.2% sodium chloride solution was added and incubated at 37 ° C. for 24 hours for sensory evaluation. The results are shown in FIG.
[0028]
(How to collect sweat)
The armpits of 25 general men were wiped with absorbent cotton moistened with 1.5 mL of distilled water. Absorbent cotton was squeezed, and the collected product (50 mL) was filtered through a 0.45 μm filter, then filtered through MILLIPORE Centriprep YM-10, and lyophilized. To this was added 10 mL of 50 mM citrate buffer (pH 5.0) to make sweat.
[0029]
【The invention's effect】
Since the deodorant agent and β-glucuronidase inhibitor of the present invention can continuously suppress the generation of unpleasant body odor without killing resident bacteria of the skin, a cosmetic having an excellent deodorizing effect and high safety, It is useful as a topical medicine or quasi-drug.
[Brief description of the drawings]
FIG. 1 is a diagram showing the influence of plant and fungal extracts on skin resident bacteria.
FIG. 2 is a graph showing the β-glucuronidase activity inhibition rate of plant and fungal extracts.
FIG. 3 is a diagram showing changes in sweat odor caused by β-glucuronidase.
FIG. 4 is a diagram showing an effect of inhibiting sweat odor production by β-glucuronidase inhibition.

Claims (1)

Gardenia, Tsu boxer, comfrey, Se Nburi, Cordyceps and Iraq Sa or al plants and fungi are selected, or consists of extracts β- glucuronidase inhibitor.

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