JP4728808B2 - Insecticide - Google Patents

Description

  The present invention relates to an insecticide, and more particularly to an insecticide having an excellent control effect and persistence against pests that are particularly difficult to control in the field of agriculture and horticulture.

  Macrolide compounds produced by soil actinomycetes are widely used as pest control agents because they exhibit insecticidal activity at low concentrations in a wide range of pests. Representative macrolides currently used include spinosad (trade name: Spinoace), emamectin benzoate (trade name: Afarm), milbemectin (trade name: Colomite), nemadectin (trade name: Megatop) And the like. However, although the insecticidal activity is wide, it has been reported that some compounds have a certain degree of selectivity in the insecticidal activity (see, for example, Non-Patent Document 1).

“Plant Protection”, Vol. 54, No. 9 (2001), P377 In addition, to date, there have been known insecticides and acaricides with starch and spinosad or emamectin benzoate having excellent control effect. It has been. (For example, see Patent Document 1).

JP-A-2001-302416 (Claim 1) These insecticides and acaricides are drugs intended to improve the ability of insecticidal insects and maintain their sustainability by mixing them at normal use concentrations. However, in reality, there is a problem that the insecticidal power is weak against pests flying after spraying and larvae hatched after spraying.

  An object of the present invention is to solve such a conventional problem and to provide an insecticide that exhibits high insecticidal activity at a low active ingredient concentration and is excellent in the sustainability of the insecticidal activity.

  In order to solve the above-mentioned problems, the present inventors have repeatedly studied the components constituting the insecticide, and as a result, as this component, a fatty acid glyceride originally having low insecticidal activity alone and a macrolide insecticide. It was found that the above-mentioned problems can be solved unexpectedly by using them in combination with insect pests having resistance to insecticidal activity and / or persistence, and the present invention has been completed based on this finding. It was.

That is, the means of the present invention for solving the problems of the present invention are as follows:
(Hereinafter, sometimes simply referred to as a fatty acid glyceride.) Capric acid and mixed fatty and fatty acid triglyceride obtained from glycerol caprylic at a rate of concentration 200～3000Ppm, and spinosad, emamectin benzoate, milbemectin and a agricultural or horticultural insecticide characterized by containing at least one macrolide insecticide selected from the group consisting of nemadectin at the rate of concentration 0.2～100Ppm.

Preferred embodiments of the means for solving the problems of the present invention include the following agricultural and horticultural insecticides .
Agricultural and horticultural insecticides for the control of spider mite, semi-lepidopterous, total lepidopterous or lepidopterous pests.

  According to this invention, a fatty acid glyceride and a macrolide obtained from a low-concentration C8-22 fatty acid against pests that do not exhibit insecticidal activity with macrolide insecticides or pests that are less effective at low concentrations. A specific insecticidal action was observed in each process of growing from the egg of the target pest to the adult by mixing with the insecticide. In other words, after spraying, for the surviving adult worms and spawned eggs, an ovicidal action, an egg-laying-inhibiting action, an feeding-stopping action, and an improvement in the ability to kill insects are seen, resulting in a high pest control effect, There is a tremendous contribution to pest control in the field of agriculture and horticulture.

  This invention is an insecticide containing a fatty acid glyceride and a macrolide insecticide.

  The fatty acid glyceride used in this invention is a glycerin ester of a fatty acid having 8 to 22 carbon atoms and glycerin. The fatty acid forming the fatty acid glyceride may be a natural fatty acid or a synthetic fatty acid, but is preferably a fatty acid derived from a plant, for example, soybean, cottonseed or rapeseed. Examples of fatty acids having 8 to 22 carbon atoms include caprylic acid, pelargonic acid, capric acid, undecanoic acid, lauric acid, tridecanoic acid, myristic acid, pentadecanoic acid, palmitic acid, margaric acid, stearic acid, nonadecanoic acid, arachidic acid, heneicosane An acid, behenic acid, etc. can be mentioned, Especially, a caprylic acid, capric acid, and a mixture thereof are preferable.

  The fatty acid glyceride can be produced by esterification or transesterification from the fatty acid and glycerin by a conventional method. The fatty acid glyceride to be produced includes fatty acid monoglyceride, fatty acid diglyceride or fatty acid triglyceride, and any fatty acid glyceride can be used. Of these, glycerides of mixed fatty acids of caprylic acid and capric acid (decanoyl octanoyl glycerol, trade name: Sun Crystal Emulsion) are preferable.

  Examples of the macrolide insecticide used in the present invention include insecticides comprising macrolide compounds produced by soil actinomycetes, such as spinosad (trade name: Spinoace), emamectin benzoate (trade name: Afarm), milbemectin. (Trade name: colomite) or nemadectin (trade name: Mega Top).

  The insecticide of this invention can be prepared, for example, by mixing the fatty acid glyceride and the macrolide insecticide. In the preparation, when the fatty acid glyceride is solid at room temperature, it may be mixed with the macrolide insecticide by heating and melting it, and then mixed with the macrolide insecticide and the fatty acid glyceride and the fatty acid. A solution or suspension may be prepared by mixing glyceride, and this solution and a macrolide insecticide may be mixed. Examples of the solvent include known solvents that can dissolve fatty acid glycerides, and specifically include solvents such as methanol, ethanol, isopropanol, propylene glycol, glycerin, sorbitol, or mixed solvents thereof. Can do. When the fatty acid glyceride is liquid at room temperature, the fatty acid glyceride and the macrolide insecticide are preferably mixed and prepared at room temperature or with heating. In preparation of mixing, emulsifiers, dispersants, adjuvants and the like known in the technical field of this insecticide can be appropriately added to and mixed with the fatty acid glyceride and the macrolide insecticide.

  The macrolide insecticide particles used at this time are preferably granular, and the particle size of the macrolide insecticide when used in a granular form is not particularly limited. Moreover, there is no restriction | limiting in the conditions which mix macrolide insecticide particle | grains.

  There is no particular restriction on the mixing ratio of the fatty acid glyceride and the macrolide insecticide.

  The insecticide of the present invention can be produced by adding auxiliary agents such as emulsifiers, dispersants and fixing agents to fatty acid glycerides and macrolide insecticides.

  The insecticide of this invention thus produced can be used as it is, but it is preferable to dilute with water or the like for use such as spraying.

  In the dilution, a fatty acid glyceride diluted by adding water or the like and a macrolide insecticide diluted by adding water or the like may be mixed. There is no limit to the degree of dilution. The concentration of the fatty acid glyceride in the insecticide diluted in this way is usually 200 to 3000 ppm, and the concentration of the macrolide insecticide is usually 0.2 to 100 ppm.

  Examples of target pests to be controlled by the insecticide of the present invention include spider mite, apple spider mite, spider mite, Kanzawa spider mite, etc. Lepidopterous insects such as whitefly insects (such as white fly whitefly, silver leaf whitefly, tobacco whitefly), thrips (such as white thrips, citrus thrips, chano thrips, etc.), lepidoptera Can do.

  The insecticide of the present invention has a specific insecticidal action against such pests in each process of growing from the eggs to adults. On the other hand, an egg-killing action, an egg-laying-inhibiting action, a feeding-stopping action and an improvement in the ability to kill insects are observed. In particular, when the concentration is as low as 0.2 to 100 ppm, a macrolide insecticide that originally does not exhibit insecticidal activity exhibits a high insecticidal control effect when mixed with a fatty acid glyceride.

  Hereinafter, the present invention will be described in more detail with reference to examples. However, the present invention is not limited to these examples.

Example (Example of production of insecticide)
Sun crystals containing 90% decanoyl octanoyl glycerol as an active ingredient (commercial product, trade name, dosage form is emulsion) are diluted with water to the dilution factor shown in the table below. A dilution was prepared. In addition, spinoace containing 25% of spinosad as an active ingredient (commercial product, product name. Granule wettable powder) is added with water so that the dilution factor shown in the following table is added, and a spinoace diluent is added. Prepared. Next, the sun crystal diluent and the spinoace diluent were mixed to produce an insecticide.
Sun crystals diluted by adding water and diluting the sun crystals to the dilution factor shown in the table below, and diluting the afarm formulation with water to the dilution factor shown in the table below The pesticide was prepared by mixing with the prepared Afarm diluent.
Sun crystals diluted by adding water to dilute the sun crystal to the dilution factor shown in the following table, and dilute the colomite emulsion with water to the dilution factor shown in the following table. The colomite dilution prepared in this way was mixed to produce an insecticide.
Add Sun Crystal Diluent prepared by diluting by adding water to the dilution factor shown in the table below, and add water to Mega Top Solution to the dilution factor shown in the table below. The pesticide was produced by mixing with the megatop diluent prepared by dilution.

Comparative Example 1 (Insecticide production example)
The sun crystal diluted solution obtained by diluting the sun crystal with the dilution water shown in the following table was used as an insecticide, and this insecticide was indicated as “Comparative Example 1” in the table. Sun crystals diluted by adding water to dilute the sun crystal to the dilution factor shown in the table below, and dilute by adding water to the cascade emulsion to the dilution factor shown in the table below. To produce a pesticide. This insecticide was indicated as “Comparative Example 1 + K” in the table. Add the sun crystal diluted solution prepared by adding water and diluting it to the dilution factor shown in the following table, and add water to the Dantotsu water solvent to the dilution factor shown in the following table. The insecticide was manufactured by mixing with the diluted solution prepared by dilution. This insecticide was indicated as “Comparative Example 1 + D” in the table. Add Sun Crystal Diluent prepared by diluting Sun Crystal to the dilution factor shown in the table below, and add water to Oltran Wettable Powder to the dilution factor shown in the Table below. The pesticide was produced by mixing with the ortho-diluted solution prepared by dilution. This insecticide was indicated as “Comparative Example 1 + R” in the table.

Comparative Example 2
A spinoace dilution obtained by diluting spinoace with water having a dilution ratio shown in the following table was used as an insecticide, and this insecticide was indicated as “Comparative Example 2” in the table. A mixed solution of the spinoace dilution and an adhesive solution diluted by diluting the adhesive solution with water so as to have a dilution factor shown in the table was used as an insecticide. 2 + N ”. A mixture of the Spino Ace diluted solution and an oleate diluted solution obtained by diluting an oleate solution with water so as to have a dilution factor shown in the table was used as an insecticide, and this insecticide was referred to as “Comparative Example 2 + O” in the table. displayed. A mixture of the spinoace dilution and an Akaritouch dilution obtained by diluting the Akaritouch emulsion with water so as to have a dilution factor shown in the table was used as an insecticide, and this insecticide was referred to as “Comparative Example 2 + A” in the table. Is displayed.
Comparative Example 3
An afarm diluted solution prepared by adding water to the afarm formulation to dilute it to the dilution factor shown in the following table and diluting was used as an insecticide, and this was indicated as “F” in the table. A colomite diluted solution prepared by adding water to the colomite emulsion so as to have a dilution factor shown in the following table and diluting was used as an insecticide, and this was indicated as “C” in the table. A cascade dilution prepared by adding water to the cascade emulsion to dilute in the following table and diluting was used as an insecticide, and this was indicated as “K” in the table. A dantotsu dilute solution prepared by adding water and diluting the dantotsu wettable powder to the dilution factor shown in the following table was used as an insecticide, and this was indicated as “D” in the table. An ortho-diluted solution prepared by diluting the ortho-run wettable powder with water so as to have a dilution factor shown in the following table was used as an insecticide, and this was indicated as “R” in the table. A megatop diluted solution prepared by adding water and diluting the megatop solution so as to have a dilution factor shown in the following table was used as an insecticide, and this was indicated as “M” in the table. An adhesive solution diluted by diluting the adhesive solution with water so as to have a dilution factor shown in the table was used as an insecticide, and this insecticide was indicated as “N” in the table. An oleate diluted solution obtained by diluting an oleate solution with water so as to have a dilution factor shown in the table was used as an insecticide, and this insecticide was indicated as “O” in the table. An Akaritouch diluted solution obtained by diluting an Akaritouch emulsion with water so as to have a dilution factor shown in the table was used as an insecticide, and this insecticide was indicated as “A” in the table.

Evaluation tests 1-6
Release adult female nymph mite on the leaf of pot-grown cucumber (variety: Honor, 1.5 leaf stage), investigate the number of parasites two days after adult female colonization, and use the dilution factor shown in Table 1. The diluted insecticide was sprayed with a small sprayer. The status of life and death of adult females one day after spraying was investigated. The results are shown in Table 1.

  As can be seen from the results of Comparative Examples 1 and 2 as shown in Table 1, as for the effect of spinoace on adult female spider mites, there are many paralytic insects, there are difficulties in quick action, and the insecticidal power is low. there were. On the other hand, in the sun crystal emulsion, there are many live insects and the killing power was not high. However, by combining spinoace and sun crystal, it was confirmed that the rapid action and the insecticidal power were improved. In this test, the rapid effect on adults one day after spraying was observed. Usually, paralytic insects are divided into life and death in 3 to 4 days. Even in the state of paralysis, it has the ability to lay eggs depending on the degree thereof, and thus the immediate effect on adults is extremely important for the acaricide.

Evaluation test 7-12
Insects shown in Table 2 were sprayed with a small sprayer at the dilution factor shown in Table 2 (dilution with water) on green beans grown in pots (variety: Satsuki Midori No. 2, first compound leaf stage). After spraying, it was managed in a glass house, and after 20 days and 1 day after spraying, 20 adult spider mite were released on the first compound leaf of green beans. Three days after the release, the number of living females and the total number of eggs laid were examined. The results are shown in Table 2.

  Figures in parentheses are the number of eggs laid per surviving insect.

  As can be seen from the results of the examples and comparative examples in Table 2, by using spinoace and sun crystal together, an improvement in insecticidal power and a decrease in the number of eggs laid were observed as compared to the case where each was used alone. . This tendency is maintained even against the larvae 4 days after spraying, and an improvement in residual effect can be expected. After spraying, the number of eggs produced by the released adults in 3 days is 22-24 per head in the non-sprayed area, 19-23 by using Spinoace alone, and 115-116 by using Sun Crystal alone. became. In the mixed-use area, the number of eggs is 4 to 8 including the low-concentration area. After spraying, it is determined whether the released insects are in contact with the insecticide attachment sites on the leaves or due to the phytotoxic effect of sucking juice. However, many dead solids were observed in 3 days, and the number of laying eggs of living solids decreased. From the aspect of control, the mite-killing effect is observed when ticks on the back of leaves that are difficult to be applied with insecticide, or when mites flying by wind come into contact with the insecticide-treated leaves, have a residual effect. Is also important.

Evaluation test 13-19
After releasing 20 adult spider mite every 20 days to a pot-grown kidney bean (variety: Satsuki Midori No. 2, first compound leaf stage), and spawning in a glass house for 2 days, the insecticides shown in Table 3 It sprayed with the small sprayer by the dilution factor (dilution with water) shown in Table 3. Seven days after spraying, the number of parasitic spider mites was examined at each growth stage. The results are shown in Table 3.

  As shown in Table 3, the spinoace granule wettable powder and the sun crystal emulsion alone showed the ability to kill insects, but there were many nymphs grown from hatched larvae. By combining spinoace and suncrystal, the ovicidal power was improved, and further, the growth of hatched larvae was stopped. In addition, the growth stage under 27 degreeC conditions of the Kanzawa spider mite similar to a spider mite is as shown in Table 4.

  That is, the number of days from egg laying to adults is 10 days. In this test, adults and eggs laid for 2 days were sprayed as targets. After spraying, the surviving adults continue to lay eggs. Larvae hatched from eggs (six feet) enter the first stationary phase and molt and grow into nymphs (eight feet). Larvae hatched from each single-use section grew normally and most became nymphs. Due to the combined use of Spinoace and Sun Crystal, hatched larvae were found to be starved dead due to feeding inhibition in the mixed area. In the mixed area, hatched larvae are seen, but as will be described later, they starve to death as the days pass.

Evaluation test 20-25
Twenty adult females of the spider mite were released on the primary leaves of a pot-grown kidney bean (variety: Satsuki Midori No. 2). After laying eggs for 2 days, the insecticide shown in Table 5 was sprayed with a small sprayer at the concentrations shown in Table 5. 5, 7, and 10 days after spraying, the green leaves of green beans were cut out from each pot, and the number of live larvae hatched was examined. The results are shown in Table 5.

Figures in parentheses are growth inhibition rates.
Growth inhibition rate = 1− (number of live insects in sprayed area / number of live insects in non-sprayed area) × 100

  The spinoace granule wettable powder and sun crystal emulsion each showed a tendency to increase the number of larvae as the number of days passed after spraying. However, when spinoace and suncrystal were mixed, many dead solids were observed due to the feeding-inhibiting effect on hatched larvae, and the growth of urticae was completely suppressed after 10 days.

Evaluation test 26-41
Ten adult spider mites were released on the fresh leaves of pot-grown kidney beans (variety: Satsuki Midori No. 2) and allowed to lay eggs in a glass house for 3 days. Three days after the release, the insecticide shown in Table 6 was sprayed with a small sprayer in the dilution ratio (dilution with water) and concentration shown in Table 6. The growth inhibition rate was calculated from the number of parasites 9 days after spraying. The results are shown in Table 6.

  Growth inhibition rate = 1− (number of live insects in sprayed area / number of live insects in non-sprayed area) × 100

  When spinoace and sun crystal were used alone, a large number of surviving insects were seen, but by using spinoace and sun crystal together, the surviving insects were drastically reduced and a high synergistic effect was observed. The mixed concentration (ppm) ratio at which this effect was exhibited was spinoace: suncrystal = 1: 8 to 1: 250. Further, the complete blocking mixture concentration ratio was 1:58.

Evaluation test 42-49
Planter-grown eggplants (variety: Senryo No. 6 leaf stage) were inoculated with a spider mite parasitic leaf section. Three days after the inoculation, the number of nymph mite parasites before spraying the insecticide was investigated, and then the insecticide shown in Table 7 was sprayed with a small sprayer at the dilution factor shown in Table 6 (dilution with water). The number of parasites after 7, 14, 20, and 29 days after spraying was investigated for all leaves, and the control efficiency was calculated from the number of parasites. The results are shown in Table 7.

Control efficiency = (1−CbΣTai / TbΣCai) × 100
Cb: Number of insects before spraying insecticide in the non-sprayed area Tai: Number of insects for the i-th time after spraying the insecticide in the sprayed area Tb: Number of insects before spraying the insecticide in the sprayed area Cai: After spraying the insecticide in the non-sprayed area I-th insect number

  When Spinoace and Sun Crystal were each used alone, the effect on the spider mite was inferior, but by using Spino Ace and Sun Crystal together, an improvement in the effect was recognized.

Evaluation test 50-61
Twenty adult female nymph mites were released on the first compound leaf of pot-grown kidney beans (variety: Satsuki Midori No. 2) and allowed to lay eggs in a glass house for 1 day. After investigating the number of nymph mite parasites before spraying the insecticide, the insecticide shown in Table 8 was sprayed with a small sprayer at the dilution factor shown in Table 8 (dilution with water), and the number of parasites 7 days after spraying was examined. . The results are shown in Table 8.

N: Adhesive solution (Sumitomo Chemical Co., Ltd., 5% starch)
O: Olate solution (Otsuka Chemical Co., Ltd., 20% sodium oleate)
A: Akari touch emulsion (Ishihara Sangyo Co., Ltd., 70% propylene glycol mono fatty acid ester)

  In Table 8, “Comparative Example 2 + N” for the insecticide indicates that the insecticide is a mixture of the insecticide prepared in Comparative Example 2 and an adhesive liquid. The indications “Comparative Example 2 + O” for the insecticide and “Comparative Example 2 + A” for the insecticide indicate the same thing.

  Adhesive solution, oleate solution or Akaritouch emulsion which is a physical control agent and spinoace were not mixed. When spinoace and sun crystal were used alone, the miticide effect was inferior, but by combining spinoace and sun crystal, many dead solids of hatching larvae were seen, and other similar It was found that the action and effect different from the physical control agent can be obtained.

Evaluation test 62-97
Leaves (one branch, one leaf) were collected from an outdoor mandarin orange tree (one mandarin orange), washed with water and inserted with water, and then 10 adult mandarin mite mites were released per leaf. After laying eggs in a room at 25 ° C. for 4 days, adult females were removed and the number of eggs laid was investigated. On the same day, the insecticide shown in Table 9 was sprayed with a small sprayer in the dilution ratio (dilution with water) and concentration shown in Table 9. After spraying, it was placed in a room at 25 ° C., and the mortality of hatching larvae 8 days after spraying was calculated. The results are shown in Table 9.

  When spinoace and suncrystal were each used alone, there was almost no larvicidal effect, but by mixing spinoace and suncrystal, a lot of starvation solids were seen, and the effect on larvae was significantly improved. Admitted. At first glance, the mortality rate seems to be low compared with the growth inhibitory effect and control effect, but as the number of days passes, the number of starved deaths increases. Moreover, in the tested ovicidal effect, the effect by mixing was not recognized, but it is the most characteristic point that the larva hatched by mixing does not grow. This action and effect is not observed when sun crystals are used alone, but differs only in that they are expressed only when sun crystals are sprayed on larvae by continuous spraying.

Evaluation test 98-132
Sprinkling potted green beans (variety: Satsuki Midori No.2) with fresh insecticides shown in Table 10 using a small sprayer at the dilution factor (dilution with water) and concentration shown in Table 10 and placing them in the area where the legumes occur did. After investigating the number of beetle-feeding insects that had occurred 14 days after spraying, the damage prevention rate was calculated. The results are shown in Table 10.

Damage prevention rate = (1−number of parasites in sprayed area / number of parasites in non-sprayed area) × 100
Spinoace is known to have high direct insecticidal activity against larvae that feed on larvae, but after spraying as in this test, no effect on parasitic larvae or hatched larvae has been observed. Even when using Sun Crystal alone. It is the same. It was confirmed that by combining Spinoace and Sun Crystal, the effect on hatching larvae was enhanced, but a high damage prevention effect was obtained, although eggs were laid. After spraying, when it is placed in the area where the leguminous fly flies are frequent, by mixing spinoace and sun crystal, adult dead can be seen, but there are many food marks and number of eggs laid. By the time hatching begins, small marks of hatched larvae are observed, but after hatching and feeding damage, the larvae die. Long aftereffect. At present, there are few drugs that show damage-preventing effects with special agents for legumes.

Evaluation tests 133-146
Planter-grown cherry tomatoes (variety: Tiny Tim 6.5 leaf stage, 10 plants planted), sprayed with the insecticide shown in Table 11 using a 500 ml vertical sprayer per planter at the dilution factor shown in Table 11 (dilution with water) did. Eight days after spraying, the damage to the top five leaves (50 leaves in 1 ward) was investigated. The damage leaf rate, damage level and control value were calculated from the extent of this damage. The results are shown in Table 11.

F: Afarm emulsion (manufactured by Syngenta Japan, 1% emamectin benzoate)
C: Colomite emulsion (Sankyo Co., Ltd., 1% milbemectin)
K: Cascade emulsion (manufactured by BASF Agro, 10% flufenoxuron)
D: Dantotsu water solvent (Takeda Pharmaceutical Co., Ltd., 16% clothianidin)
R: Ortran wettable powder (Takeda Pharmaceutical Co., Ltd., 50% acephate)
Number of surveyed leaves: 150
Degree of damage 0: No infiltration mark.
1: Submarine trace is less than 10% of the total area.
2: Insidation trace is less than 10 to 25% of the total area.
3: Submerged trace is less than 25 to 50% of the total area.
4: Submarine trace is 0% or more of the total area.
Damage degree _{= n + 2n 2 + 3n 3} + 4n 4/4 × N
n: Number of leaves indicated by the degree of damage.
N: Number of leaves surveyed.
Damage value = Degree of damage in untreated area-Degree of damage in treated area / Degree of damage in untreated area x 100

  It was confirmed that a high control effect can be obtained by mixing a macrolide insecticide and a sun crystal emulsion.

Evaluation test 147-159
Pot-grown kidney beans (variety: Satsuki Midori No. 2) The insecticide shown in Table 12 was sprayed with a small sprayer at the concentrations shown in Table 12 to the southern thrips larvae parasitized in the first compound leaf stage. The life-and-death situation of the southern blue thrips parasitizing 5 days after spraying was investigated, and the death rate was calculated. The results are shown in Table 12.

  When spinoace was used alone, it was confirmed that a high control effect was obtained by mixing spinoace and sun crystal in a concentration range of 12.5 ppm or less, which is inferior in effect.

Evaluation test 160-164
Potted kidney beans (variety: Toramaru Uzura) primary leaves were placed in a breeding cage (30 × 25 × 28 cm), and adult white lice were released, and eggs were laid for 2 days. After egg laying, the insecticide shown in Table 13 was sprayed with a small sprayer at the dilution factor shown in Table 13 (dilution with water). Two days after spraying, the parasitic leaves were cut out and the hatching status and the death rate of hatched larvae were investigated using a microscope. The results are shown in Table 13.

  It was recognized that the effect on hatched larvae was remarkably improved by mixing Spinoace and Sun Crystal, and a stable effect was obtained.

Evaluation test 165-200
The first true leaf of the pot-grown cucumber (variety: Shinko No. A) was inoculated with a cotton aphid parasitic leaf, and the number of parasites was examined one day later. After the investigation, the insecticide shown in Table 14 was sprayed with a small sprayer in the dilution ratio (dilution with water) and concentration shown in Table 14. Five days after spraying, the number of parasites was investigated and the growth inhibition rate was calculated. The results are shown in Table 14.

Growth inhibition rate = (1−number of pre-treatment insects in untreated group × number of treated insects in treated group / of treated group
Number of worms before treatment x number of worms after treatment in untreated section) x 100

The effect of spinoace alone was not observed, but the effect of inhibiting the growth of cotton aphids was enhanced by the combination of spinoace and suncrystal.

Evaluation test 201-230
20 adults of two spider mites (2 pots) were released on the primary leaves of pot-grown kidney beans (variety: Satsuki Midori 2). After laying eggs for 2 days, the insecticide shown in Table 15 was sprayed with a small sprayer at the concentrations shown in Table 15. Nine days after spraying, the number of surviving insects was examined, and the growth inhibition rate was calculated. The results are shown in Table 15.

Growth inhibition rate = (1−number of live insects in sprayed area / number of live insects in non-sprayed area) × 100
C: Colomite emulsion (Sankyo Co., Ltd., 1% milbemectin)
A stable effect was obtained by mixing a colomite emulsion and a sun crystal emulsion at a concentration of 1 ppm or less, which is inferior in effect with a single chromite emulsion. The optimum mixing ratio was colomite: sun crystal = 1000 ppm: 1.13 ppm (1: 885).

Evaluation tests 231 to 238
The insecticides shown in Tables 16 and 17 were sprayed on the first true leaves of pot-cultivated cucumbers (variety: Shinko No. A) with a small sprayer in the dilution ratios shown in Tables 16 and 17 (dilution with water). Two, four, six and eight days after spraying, 15 adult spider mite were released on the surface of the cucumber leaves. The number of surviving insects and the number of eggs laid in the spider mite that moved to the back of the leaf 2 days after each release was investigated. Figures in parentheses are the number of eggs laid per head. The results are shown in Table 16 and Table 17.

C: Colomite emulsion (Sankyo Co., Ltd., 1% milbemectin)
The first true leaf of the sprayed cucumber stretched 2.5 times 8 days after spraying. After spraying, the effect of the colomite emulsion on adult worm spider mites was not significant. The number of eggs laid by adults that moved from the leaf surface to the back of the leaf decreased to 4 days after spraying when colomite emulsion was used alone, and an egg-laying inhibitory effect was observed. By mixing the colomite emulsion and the sun crystal emulsion, the number of eggs laid was further reduced, and the egg-laying inhibitory effect could be maintained until 8 days after spraying.

Evaluation test 239-263
20 adults of two spider mites (2 pots) were released on the primary leaves of pot-grown kidney beans (variety: Satsuki Midori 2). One day after the release, the insecticide shown in Table 18 was sprayed with a small sprayer in the dilution ratio (dilution with water) and concentration shown in Table 18. Adult larvae and hatched larvae 7 days after spraying were investigated, and the growth inhibition rate was calculated. The results are shown in Table 18.

F: Afarm emulsion (manufactured by Syngenta Japan, 1% emamectin benzoate)
Growth inhibition rate = (1−number of pre-treatment insects in untreated group × number of treated insects in treated group / of treated group
Number of worms before treatment x number of worms after treatment in untreated section) x 100
Number of surviving insects in untreated section: 12 adults, 28 larvae, total 40

  Although the growth inhibitory effect was high even with the single use of the afarm emulsion, it was confirmed that a high effect was obtained by mixing the afarm emulsion and the sun crystal emulsion in the 1.62 ppm section of the inferior afarm.

Evaluation test 264-288
20 adults of two spider mites (2 pots) were released on the primary leaves of pot-grown kidney beans (variety: Satsuki Midori 2). One day after the release, the insecticide shown in Table 19 was sprayed with a small sprayer in the dilution factor (dilution with water) and concentration shown in Table 19. Adult larvae and hatched larvae 7 days after spraying were investigated, and the growth inhibition rate was calculated. The results are shown in Table 19.

M: Megatop liquid (manufactured by BASF Agro Co., Ltd., 3.6% nemadectin)
Growth inhibition rate = (1−number of pre-treatment insects in untreated group × number of treated insects in treated group / of treated group
Number of worms before treatment x number of worms after treatment in untreated section) x 100

  The effect of the megatop solution alone was unstable, but it was confirmed that a stable effect could be obtained by mixing the megatop solution and suncrystal.

Evaluation test 289-324
The insecticide shown in Table 20 was sprayed on the green beans (variety: Satsuki Midori No. 2) grown in pots with a small sprayer in the dilution factor (dilution with water) and concentration shown in Table 20. After spraying, they were placed in a cherry tomato cultivation house where peas were frequently spawned and spawned. 13 days after spraying, the number of legume larvae parasitic on the primary leaves was investigated, and the damage prevention rate was calculated from the number of parasites. The results are shown in Table 20.

F: Afarm emulsion (manufactured by Syngenta Japan, 1% emamectin benzoate)
Damage prevention rate = (1−number of parasites in sprayed area / number of parasites in non-sprayed area) × 100

  In the single use of the Afarm emulsion, a high effect was observed at 2000 times (5 ppm), but no effect was shown at a concentration lower than this. In a concentration group of 2.5 ppm or less where the effect is inferior, the damage prevention effect was remarkably improved by using the mixture of the Afama emulsion and sun crystal.

Evaluation test 325-334
Pot aphid parasites were inoculated into pot-grown cucumbers (variety: Shinko No. A, 2-leaf stage). After investigating the number of insects before spraying 2 days after inoculation, the insecticide shown in Table 21 was sprayed with a small sprayer at the dilution factor shown in Table 21 (dilution with water). Three days after spraying, the number of parasites was investigated and a corrected density index was calculated. The results are shown in Table 21.

C: Colomite emulsion (Sankyo Co., Ltd., 1% milbemectin)
F: Afarm emulsion (manufactured by Syngenta Japan, 1% emamectin benzoate)
Corrected density index = number of pre-treatment insects in the untreated group x number of treated insects in the treated group / number of pre-treated insects in the treated group x none
Number of insects after treatment in the treatment area

  The use of each of the chromite emulsion and the afarm emulsion was inferior in effect, but it was confirmed that a high effect was obtained by mixing the colomite emulsion or the afarm emulsion with a 900-fold diluted solution of sun crystal. .

Evaluation test 335-343
The insecticide shown in Table 22 was sprayed with a small sprayer at the dilution factor shown in Table 22 (dilution with water) on the new leaf parts damaged by the dust mite parasitized in the field-grown eggplant (variety: Senryo 2). Three days after spraying, the new leaf part sprayed was cut out, and the number of live insects was examined under a microscope. The results are shown in Table 22.

  C: Colomite emulsion (Sankyo Co., Ltd., 1% milbemectin)

It was found that the use of each of the colomite emulsion and the ardent wettable powder alone is highly effective by mixing the colomite emulsion with a 600-fold diluted solution of sun crystal at 16000 times and 32000 times that the effect is inferior. .

Claims (2)

The fatty acid triglyceride obtained from the mixed fatty acids and glycerin capric acid and caprylic acid at a ratio of concentrations 200～3000Ppm, and spinosad, emamectin benzoate, at least one of the macros that are selected from the group consisting of milbemectin and nemadectin An agricultural and horticultural insecticide comprising a ride-type insecticide at a concentration of 0.2 to 100 ppm. The insecticide for agricultural and horticultural use according to claim 1, which is for controlling spider mite insects, semilepidopterous insects, total lepidopterous insects or lepidopterous insects.