JP4817698B2 - Method of operating blood component collection device - Google Patents

Description

The present invention relates to a method for operating a blood component collection device.

  At the time of blood collection, for the purpose of effective use of blood and reduction of burden on blood donors, the blood sample is separated into each blood component by centrifugation, etc., and only the components necessary for the transfuser are collected. Ingredients are collected to return the ingredients to the blood donor.

  In such component blood collection, for example, when obtaining a plasma preparation, blood collected from a blood donor is introduced into a blood component collection circuit, and plasma and a plasma are collected by a centrifuge called a centrifuge bowl installed in the blood component collection circuit. It is separated into blood cells, and the plasma therein is collected in a blood component collection bag to obtain a plasma preparation, and the blood cells are returned (returned) to the blood donor together with the remaining plasma.

  By the way, in a blood component collection apparatus that collects such component blood, for example, when collecting plasma, a blood component collection step for collecting plasma, and a blood component return for returning the remaining blood components (mainly blood cells) to the donor The process is performed alternately (see, for example, Patent Document 1).

  However, in the blood component collection device described in Patent Document 1, for example, when the amount of plasma collected in one cycle is increased, blood cells adhere to the outlet of the centrifuge in the blood component collection step. If it is not removed, the adhered blood cells will be transferred into the blood component collection bag in the next blood component collection step.

JP 2001-17540 A

An object of the present invention is to provide a blood component collection device that can prevent unnecessary blood components (for example, blood cells) from being mixed into a predetermined blood component (for example, plasma) to be collected without increasing the required time . It is to provide an operating method .

Such an object is achieved by the present inventions (1) to (6) below.
(1) Blood collection means for collecting blood from a donor,
A centrifuge that includes a rotor having a blood storage space therein, and centrifuges blood collected by the blood collection means by rotation of the rotor in the blood storage space;
A blood collection / return line connecting the blood collection means and the centrifuge;
A blood component collection bag that communicates with the blood storage space and collects a predetermined blood component separated by the centrifuge;
A blood component collection circuit comprising a storage bag communicating with the blood storage space ;
A liquid feeding means provided on the blood collection / return line;
Channel opening and closing means provided on the blood collection / return line;
Control means for controlling the liquid feeding means and the flow path opening and closing means ;
A plurality of cycles of blood component collection operations comprising: a blood component collection step of centrifuging blood collected from a donor and collecting the predetermined blood component; and a blood component return step of returning the remaining blood components to the donor A method of operating a blood component collection device to perform,
In the blood component collection step in the blood component collection operation of each cycle after the second cycle , the control means controls the predetermined amount before collecting the predetermined blood component in the blood component collection bag. Performing an initial flow removal operation of transferring the predetermined blood component of the storage bag to the storage bag,
In the blood component return step in the blood component collection operation of each cycle from the second cycle to the last cycle, the predetermined blood component in the storage bag is returned to the blood storage space, and initially the rotor By continuing the return of the remaining blood components while stopping the rotation and rotating the rotor from the middle so that the predetermined blood components are accumulated on the outer peripheral side in the blood storage space by centrifugal force The method for operating a blood component collecting apparatus, wherein the blood component return step is terminated in a state where the predetermined blood component remains in the blood storage space.

(2) According to the control, the control means returns a part of the predetermined blood component in the blood component collection bag to the blood storage space in the blood component return step of the blood component collection operation. A method for operating the blood component collection device according to claim 1.

(3) In the blood component collecting step, the blood collected by the blood collecting means is transferred to the blood storing space through the blood collecting / returning line under the control of the control means. The air that was present is transferred into the storage bag,
In the blood component return step, the air in the storage bag is returned to the blood storage space as the remaining blood components in the blood storage space are returned to the blood donor under the control of the control means. A method of operating the blood component collection device according to 1) or (2).

(4) When the rotor rotates in the blood component returning step when the rotor is rotated in the middle and the predetermined blood component is accumulated on the outer peripheral side in the blood storage space, the air in the blood storage space The method for operating the blood component collection device according to any one of (1) to (3), wherein the blood component can flow into the blood collection / return line.

(5) According to the control, the control means does not rotate the rotor halfway in the blood component return step of the blood component collection operation in the final cycle. A method of operating the blood component collecting apparatus according to claim 1.

(6) In the blood component return step when the rotor is rotated halfway through the control, the control means detects the blood return amount from the start of the blood component return step by the liquid feed amount detection means. The operation method of the blood component collection device according to any one of (1) to (5), wherein the rotation of the rotor is started when the detected blood return amount reaches a predetermined amount.

  According to the present invention, prior to collecting a predetermined blood component (for example, plasma) in the blood component collection bag, a predetermined amount of the predetermined blood component is transferred from the blood storage space of the centrifuge into the storage bag. Storing. By the transfer of the predetermined blood component, blood components other than the predetermined blood component adhering to the inside of the flow path between the blood storage space and the storage bag (mainly near the outlet of the blood storage space) (for example, , Blood cells) are captured and transferred into the storage bag. Thereby, it is possible to prevent blood components (for example, blood cells) other than the predetermined blood component from entering the blood component collection bag.

  In addition, since the predetermined blood component returned in the blood storage space in the blood component return step can be left in the blood storage space without being returned to the donor, the predetermined blood component in the blood component collection step in the next cycle Collection efficiency can be improved. Moreover, the time required for the blood component return step can be shortened. Therefore, the required time as a whole can be shortened.

Hereinafter, the operation method of the blood component collection device of the present invention will be described in detail based on preferred embodiments shown in the accompanying drawings.

FIG. 1 is a plan view (partly including a block diagram) showing a blood component collection device in an embodiment of a method for operating a blood component collection device of the present invention, and FIG. 2 is provided in the blood component collection device shown in FIG. FIG. 3 is a partially broken cross-sectional view showing a state in which the centrifuge drive device is mounted on the centrifuge, FIG. 3 is a flowchart showing the control operation of the control unit in the first cycle, and FIG. 4 is the blood component return process A in FIG. 5 is a flowchart showing the control operation (subroutine) of the control unit in FIG. 5, FIG. 5 is a flowchart showing the control operation of the control unit in the cycle immediately before the second cycle to the last cycle, and FIG. 6 is the blood component return in FIG. The flowcharts showing the control operation (subroutine) of the control unit in the process B, FIGS. 7 to 12, respectively, sequentially show the state in the blood component collection circuit in the blood component return process B. FIG. 13 is a flowchart showing the control operation of the control unit in the final cycle, and FIG. 14 is a flowchart showing the control operation (subroutine) of the control unit in the blood component return process C in FIG. is there.

  Hereinafter, the case where the blood component collection apparatus 1 is applied to a plasma collection apparatus that mainly collects plasma as a predetermined blood component will be described as an example.

  A blood component collection device (plasma collection device) 1 shown in FIG. 1 includes a blood collection needle (blood collection means) 29 for collecting blood from a donor (blood donor), and a rotor 142 having a blood storage space 146 therein. A centrifuge (centrifuge bowl) 20 that centrifuges blood collected by the blood collection needle 29 by rotation in the blood storage space 146, and a blood component collection bag (plasma collection bag) that collects plasma separated by the centrifuge 20 ) 25 and a blood component collection circuit (plasma collection circuit) 2, and after centrifuging blood collected from a donor to collect a predetermined amount of plasma, leukocytes, platelets and red blood cells (remaining This is a device that returns blood components to the donor.

  The blood component collection device 1 includes a centrifuge 20, a first line (blood collection / return line) 21 for connecting (communication) a blood collection needle 29, which is a blood collection means, and an inlet 143 of the centrifuge 20. A second line 22 connected to the outlet 144 of the centrifuge 20, a third line 23 connected to the first line 21 and used, for example, for anticoagulant injection, A fourth line 24 connected in the middle of the line 22 and communicating with the outlet 144 of the centrifuge 20 via the second line 22; a plasma collection bag 25 connected to the second line 22; The blood component collection circuit 2 includes a sub bag 32 connected to the plasma collection bag 25 by a tube (flow path) 32 a and a storage bag 26 connected to the fourth line 24. A clamp (sealing means) 65 is provided in the middle of the tube 32a.

  In addition, the blood component collection device 1 includes a centrifuge drive device 10 for rotating the rotor 142 of the centrifuge 20, a first liquid feeding pump 11 for the first line 21, and a third line. 23, a first flow path opening / closing means 51 and a second flow path opening / closing means 52 for opening and closing the flow path of the blood component collection circuit 2, and a centrifuge drive A control unit (control means) 55 for controlling the apparatus 10, the first liquid feed pump 11, the second liquid feed pump 12, the first flow path opening / closing means 51, the second flow path opening / closing means 52, and the like; A turbidity sensor 14, an optical sensor 15, a weight sensor (detection means) 16, a first bubble sensor 17, a second bubble sensor 18, and a third bubble sensor (detection means) 27; And a fourth bubble sensor 28.

  When the blood component collection device 1 is used, the blood component collection bag 25, the sub bag 32, and the storage bag 26 are connected to the second line 22 and the tube 32a, respectively. And the side to which the fourth line 24 is connected, that is, the flow path side communicating with the blood storage space 146 of the centrifuge 20 (the lower side in FIG. 1) is suspended so as to be vertically downward. In this case, in the hole 251 of the blood component collection bag 25, the hole 321 of the sub bag 32, and the hole 261 of the storage bag 26, the corresponding hooks (supports) provided above the support mechanism (not shown) in the vertical direction, respectively. Part).

  For this reason, the operation | work etc. which set the blood component collection | recovery apparatus 1 can be performed easily, and the confirmation operation | work etc. can be performed easily, when the plasma is extract | collected in the blood component collection bag 25. FIG.

  In the present embodiment, the third line 23 and the second liquid feeding pump 12 constitute an anticoagulant supply means for adding an anticoagulant to blood collected from a donor.

Hereinafter, the structure of each part is demonstrated.
As the blood collection needle 29, for example, a metal needle is used. In the illustrated example, the blood collection needle 29 is covered with a cap.

  The first line 21 is a centrifuge-side first line to which a blood-collecting needle-side first line (blood-collecting needle-side first flow path) 21 a to which a blood collection needle 29 is connected and an inlet 143 of the centrifuge 20 are connected. It has 1 line (centrifuge separator side 1st flow path) 21b and the 1st pump tube 21g arrange | positioned among these.

  The blood collection needle side first line 21a includes a chamber 21d for removing bubbles and microaggregates.

  An air-permeable and bacteria-impermeable filter 21i is connected to the chamber 21d through a tube (flow path) 21h. This line can be used for detecting the internal pressure of the blood collection needle side first line 21a, for example.

  The second line 22 has one end connected to the outlet 144 of the centrifuge 20 and the other end connected to the plasma collection bag 25. A branch connector 61 is provided in the middle of the second line 22, that is, at a position corresponding to a cassette housing 33 described later, and is branched into three (three forks) (four connection ports in total). The second line 22 is connected via the branch connector 61.

  The branch connector 61 is connected to a breathable and bacteria-impermeable filter 63 via a tube (flow path) 62. This line can be used for detecting the internal pressure of the second line 22, for example.

  One end of the third line 23 is connected to a connecting branch connector 21 c provided on the first line 21. The third line 23 includes a second pump tube 23a, a sterilization filter (infusion filter) 64, a bubble removal chamber 23c, and an anticoagulant container connection needle 23d from the connection branch connector 21c side. In the illustrated example, the anticoagulant container connecting needle 23d is covered with a cap.

  Further, a clamp (sealing means) 66 is provided in the middle of the blood collection needle side first line 21a between the blood collection needle 29 and the connecting branch connector 21c.

  The fourth line 24 is connected to the branch connector 61. That is, the storage bag 26 is branched from the second line 22 via the fourth line 24.

  Tubes used to form the first line 21, the second line 22, the third line 23, and the fourth line 24, the pump tubes 21g and 23a, the tube 32a connected to the bag, and the chamber As a constituent material of the tube 21h connected to 21d and the tube 62 connected to the branch connector 61, polyvinyl chloride is preferable.

  If each tube is made of polyvinyl chloride, sufficient flexibility and softness can be obtained, so that it is easy to handle and is suitable for clogging with a clamp or the like.

  In addition, as each pump tube 21g and 23a, what has the intensity | strength of the grade which is not damaged even if it presses with a roller pump is used.

  Further, as the constituent materials of the connecting branch connector 21c and the branch connector 61 described above, the same constituent materials as those of the tube can be used.

  The plasma collection bag 25 is a container for collecting plasma, and is connected to the centrifuge 20 via the second line 22, whereby the inside of the plasma collection bag 25 is stored in the blood storage space 146 of the rotor 142. Communicating with

  The sub bag 32 is connected to the plasma collection bag 25 via the tube 32a, and the insides thereof communicate with each other. This subbag 32 can be used for an air vent bag, for example.

  The storage bag 26 is a container for temporarily storing (storing) air or plasma, and is connected to the branch connector 61 via the fourth line 24. That is, the storage bag 26 is connected to the centrifuge 20 via the fourth line 24, the branch connector 61, and the second line 22, whereby the inside of the storage bag 26 is stored in the blood storage space 146 of the rotor 142. Communicating with

  Each of the plasma collection bag 25, the sub bag 32, and the storage bag 26 is laminated with a resin-made flexible sheet material, and its peripheral portion is fused (heat fusion, high frequency fusion, etc.) or bonded. A bag is used.

  As a material used for the plasma collection bag 25, the sub bag 32, and the storage bag 26, for example, soft polyvinyl chloride is preferably used.

  The main part of the blood component collection circuit 2 is a cassette type as shown in FIG. The blood component collection circuit 2 partially stores and partially holds all lines (first line 21, second line 22, third line 23, fourth line 24), In other words, the cassette housing 33 in which they are partially fixed is provided.

  Both ends of the first pump tube 21g and both ends of the second pump tube 23a are fixed to the cassette housing 33. These pump tubes 21g and 23a are respectively supplied from the cassette housing 33 to the respective liquid feeds described later. It protrudes in a loop shape corresponding to the shape of pumps 11 and 12 (for example, roller pumps or the like). For this reason, the first pump tube 21g and the second pump tube 23a can be easily attached to the liquid feeding pumps 11 and 12.

  Further, the cassette housing 33 includes a plurality of openings located in the cassette housing 33. Specifically, the fourth line 24 is exposed, and the first opening 91 through which the first flow path opening / closing means 51 can enter and the second line 22 on the plasma collection bag 25 side from the branch connector 61. And a second opening 92 that allows the second flow path opening / closing means 52 to enter.

  The blood component collection device 1 includes a cassette housing mounting portion (not shown) on which the cassette housing 33 is mounted. For this reason, by mounting the cassette housing 33 to the cassette housing mounting portion of the blood component collection device 1, each line and each tube of the portion exposed from each opening 91, 92 of the cassette housing 33 automatically correspond. It is attached to each flow path opening / closing means 51, 52. As a result, the blood component collection circuit 2 can be easily mounted, and the preparation of plasma collection (blood component collection) can be performed quickly.

  In addition, the blood component collection device 1 is provided with two liquid feeding pumps 11 and 12 in the vicinity of the cassette housing mounting portion. For this reason, it is easy to attach the pump tubes 21g and 23a exposed from the cassette housing 33 to the liquid feeding pumps 11 and 12.

  The centrifuge 20 provided in the blood component collection circuit 2 is generally called a centrifuge bowl, and separates blood into plasma and blood cells (platelets, white blood cells, and red blood cells) by centrifugal force. As the centrifuge 20, the one shown in FIG. 2 is used.

  The first flow path opening / closing means 51 is provided to open and close the middle of the flow path of the fourth line 24.

  The second flow path opening / closing means 52 is provided to open and close the flow path of the second line 22 on the plasma collection bag 25 side from the branch connector 61.

  The first flow path opening / closing means 51 and the second flow path opening / closing means 52 include a line or tube insertion portion, and the insertion portion includes a drive source such as a solenoid, an electric motor, a cylinder (hydraulic pressure or air pressure), for example. It has a clamp that operates at Specifically, an electromagnetic clamp that operates with a solenoid is suitable.

  As shown in FIG. 2, the centrifuge drive device 10 includes a housing 151 that houses the centrifuge 20, a leg portion 152, a motor 153 that is a drive source, and a disk-shaped fixing that holds the centrifuge 20. It comprises a table 155. The housing 151 is placed and fixed on the upper portion of the leg portion 152.

  A motor 153 is fixed to the lower surface of the housing 151 by a bolt 156 via a spacer 157. A fixed base 155 is fitted to the tip of the rotating shaft 154 of the motor 153 so as to rotate coaxially and integrally with the rotating shaft 154, and the bottom of the rotor 142 is fitted to the upper portion of the fixed base 155. A concave portion is formed.

  The upper part 145 of the centrifuge 20 is fixed to the housing 151 by a fixing member (not shown). In the centrifuge drive device 10, when the motor 153 is driven, the fixed base 155 and the rotor 142 fixed thereto rotate under a predetermined centrifugal condition (for example, a rotational speed of 3000 to 6000 rpm). Under this centrifugal condition, a blood separation pattern (for example, the number of blood components to be separated) in the rotor 142 can be set.

  In the rotor 142, the volume of the blood storage space 146 is, for example, about 100 to 350 mL.

  Further, on the inner wall of the housing 151, an optical for optically detecting the position of the interface of blood components separated in the centrifuge 20 (blood storage space 146) (for example, the interface B between the plasma layer 131 and the blood cell layer 132). The type sensor 15 is installed and fixed by a mounting member 158.

  The optical sensor 15 includes a light source that irradiates light toward the shoulder portion of the centrifuge 20 and a light receiving unit that receives light reflected and returned from the centrifuge 20. That is, a light emitting element such as an LED (semiconductor laser or the like) and a light receiving element such as a photodiode or a CCD are arranged in a line, and the light reflected from the blood component of the light emitted from the light emitting element is reflected by the light receiving element. It is configured to receive light and photoelectrically convert the amount of received light. Since the intensity of the reflected light differs depending on the separated blood components (for example, the plasma layer 131 and the blood cell layer 132), the position corresponding to the light receiving element in which the amount of received light has changed is detected as the position of the interface B.

  More specifically, the blood cell layer 132 is obtained from the difference in the amount of light received by the light receiving unit when the position where the light of the centrifuge 20 passes is filled with plasma and when filled with the blood cell layer 132. Is detected to have reached the light passage part.

  The position where the blood cell layer 132 is detected is adjusted by changing the position where the light passes through the centrifuge (centrifugal bowl) 20, and is usually fixed after determining the light passage position.

  The weight sensor 16 constitutes detection means for detecting the amount of plasma collected in the plasma collection bag 25.

  The turbidity sensor 14 is for detecting the turbidity of the fluid flowing in the second line 22 and outputs a voltage value corresponding to the turbidity. Specifically, a low voltage value is output when the turbidity is high, and a high voltage value is output when the turbidity is low.

  Each of the first bubble sensor 17 and the second bubble sensor 18 is for detecting that air has flowed into the first line 21. That is, the presence or absence of blood in the first line 21 is detected by the first bubble sensor 17 and the second bubble sensor 18, respectively.

  The third bubble sensor 27 has air or blood in the second line 22 at a predetermined position of the second line 22 between the outlet 144 of the blood storage space 146 of the centrifuge 20 and the branch connector 61. This is for detecting the flow of (plasma). That is, the presence or absence of blood in the second line 22 is detected by the third bubble sensor 27.

  The fourth bubble sensor 28 prevents the inside of the third line 23 from being emptied (so as to be filled with the anticoagulant), so that air (anticoagulant) at a predetermined position of the third line 23 is used. This is used to detect the presence or absence of

  As the turbidity sensor 14 and the bubble sensors 17, 18, 27, 28, for example, an ultrasonic sensor, an optical sensor, an infrared sensor, or the like can be used.

  As the 1st liquid feeding pump 11 with which the 1st pump tube 21g of the 1st line 21 is equipped, and the 2nd liquid feeding pump 12 with which the 2nd pump tube 23a of the 3rd line 23 is equipped, A non-blood contact type pump such as a roller pump is suitable.

  Further, as the first liquid feeding pump (blood pump) 11, a pump capable of feeding blood in any direction is used. Specifically, a roller pump capable of forward rotation and reverse rotation is used.

  The control unit 55 includes, for example, a microcomputer and the like, and includes a centrifuge drive device 10, a first liquid feeding pump 11, a second liquid feeding pump 12, a first flow path opening / closing means 51, a second The channel opening / closing means 52, the turbidity sensor 14, the optical sensor 15, the weight sensor 16, the first bubble sensor 17, the second bubble sensor 18, the third bubble sensor 27, the fourth bubble sensor 28, etc. , Respectively, are electrically connected.

  The detection signals from the turbidity sensor 14, the optical sensor 15, the weight sensor 16, the first bubble sensor 17, the second bubble sensor 18, the third bubble sensor 27, and the fourth bubble sensor 28 are respectively controlled. Input to the unit 55 as needed. Based on the detection signals from these, the control unit 55 operates the blood component collection device 1 according to a preset program, that is, rotates, stops, or rotates in the direction of rotation (forward rotation). / Reverse rotation), and the opening / closing of the flow path opening / closing means 51 and 52 and the operation of the centrifuge drive device 10 are controlled as necessary.

  The control unit 55 includes a calculation unit and a memory (both not shown). Furthermore, the control unit 55 is electrically connected with an input unit configured with, for example, a keyboard and a display unit (none of which is shown) configured with, for example, a liquid crystal display.

  Such a control unit 55 collects blood from a donor, and controls the operation of the blood component collection apparatus 1 so as to collect plasma from the blood components (blood components).

  Specifically, the control unit 55 allows blood collected from a donor to flow into the centrifuge 20 (blood storage space 146), and collects (transfers) the plasma separated by the centrifuge 20 into the plasma collection bag 25. ) Plasma collecting step (blood component collecting step) and blood cell returning step (blood component returning step) for returning mainly blood cells (remaining blood components) remaining in the centrifugal separator 20 to the donor ( The operation of the blood component collection device 1 is controlled so as to perform the blood component collection operation.

  In the present embodiment, in the plasma collection step, the plasma to which the anticoagulant is added is collected in the plasma collection bag 25, and the weight sensor 16 detects the weight of the plasma to which the anticoagulant is added. Is done.

  The control unit 55 performs the plasma collection operation for a plurality of cycles (preferably 3 cycles or more). In the following description, the amount of plasma collected in each cycle is referred to as “the amount of plasma collected per cycle”.

  Furthermore, in the following description, the target plasma collection amount is referred to as “target plasma collection amount”, and the actually collected plasma collection amount is referred to as “current plasma collection amount”.

  Hereinafter, the usage method and operation of the blood component collection device 1 will be described with reference to FIGS.

  First, the operator (operator) primes the third line 23 and the blood collection needle 29 with an anticoagulant, and then punctures the blood collection needle 29 into the donor's blood vessel. This completes preparation for plasma collection.

  As described above, the blood component collection bag 25, the sub bag 32, and the storage bag 26 are respectively connected to the side to which the second line 22 is connected, to the side to which the tube 32a is connected, and to the fourth line 24. In the holes 251, 321, and 261, they are hooked by corresponding hooks (supports) provided on the upper side of the support mechanism (not shown) in the vertical direction so that the connected side is vertically downward. Has been lowered.

  Next, the operator turns on a collection start switch (not shown). Thereby, the program shown in FIGS. 3-6, 13 and 14 is executed, and plasma is collected.

The blood component collection device 1 performs a plasma collection step in the first cycle shown in FIG. In this plasma collection process, blood collected from a donor by a blood collection needle (blood collection means) 29 and blood mixed with an anticoagulant is introduced into the blood storage space 146 of the rotor 142 of the centrifuge 20 by an anticoagulant supply means. The plasma mixed with the anticoagulant separated by centrifuging the blood is collected in the plasma collection bag 25.
That is, in this plasma collection step, blood collection is first started (step S101).

  At the same time, the air (sterilized air) in the blood component collection circuit 2 such as the blood storage space 146 of the centrifuge 20 is transferred into the storage bag 26 via the second line 22 and the fourth line 24. Store (step S102).

  Specifically, the first flow path opening / closing means 51 is opened (opened), the second flow path opening / closing means 52 is closed (closed), and the first liquid feeding pump 11 is rotated at a predetermined rotational speed (preferably It operates (forward rotation) at about 250 mL / min or less, more preferably about 40 to 150 mL / min (for example, 60 mL / min).

  Simultaneously with the blood collection, the second liquid feeding pump 12 is operated to supply an anticoagulant such as an ACD-A solution through the third line 23, and this anticoagulant is collected into the blood sample. Add in.

  At this time, the rotation speed of the second liquid feeding pump 12 is controlled so that the anticoagulant is added at a predetermined ratio (addition ratio).

  The predetermined ratio is preferably about 1/20 to 1/6, for example, 1/12.

  As a result, blood (anticoagulant-added blood) is transferred through the first line 21 and introduced into the blood storage space 146 of the rotor 142 through the tube 141 from the inlet 143 of the centrifuge 20.

  At this time, the air in the blood storage space 146 of the centrifuge 20 is transferred and stored in the storage bag 26 via the second line 22 and the fourth line 24.

  Simultaneously with or before or after the blood collection, the centrifuge drive device 10 is operated to rotate the rotor 142 at a predetermined rotational speed.

  By the rotation of the rotor 142, the blood introduced into the blood storage space 146 is separated into two layers, a plasma layer (PPP layer) 131 and a blood cell layer 132 from the inside.

  The rotational speed of the rotor 142 is preferably about 3000 to 6000 rpm, more preferably about 4000 to 5700 rpm.

  Furthermore, when the blood collection and the supply of the anticoagulant are continued and the anticoagulant-added blood (about 270 mL) exceeding the capacity of the blood storage space 146 is introduced into the blood storage space 146, the blood storage space 146 is completely blood. And the plasma overflows from the outlet 144 of the centrifuge 20, and the plasma flows through the second line 22.

  Next, when plasma flowing out from the blood storage space 146 of the centrifuge 20 is detected by the third bubble sensor 27 (step S103), the storage of the air in the blood storage space 146 into the storage bag 26 is completed.

  Next, the first flow path opening / closing means 51 is closed, the second flow path opening / closing means 52 is opened, and the plasma in the blood storage space 146 of the centrifuge 20 is collected from the plasma via the second line 22. The sample is transferred into the bag 25 and collected (step S104).

  The weight of the plasma collection bag 25 is measured by the weight sensor 16, and the measured weight signal is input to the control unit 55.

  Next, the end of plasma collection is determined (step S105). In this case, when any one of the following (1) to (4) is detected, plasma collection is terminated.

(1) When blood cells are detected by the turbidity sensor 14.
(2) When the plasma collection amount per cycle detected by the weight sensor 16 reaches the set plasma collection amount (upper limit value) per cycle.

(3) When the current plasma collection amount (total plasma collection amount) detected by the weight sensor 16 reaches the target plasma collection amount.
(4) When the amount of blood collected per cycle reaches the allowable extracorporeal circulating blood volume.

  When any one of the above (1) to (4) is detected and the plasma collection is finished, the operation of the first liquid feeding pump 11 and the second liquid feeding pump 12 is stopped and the centrifugal separation is performed. The operation of the compressor drive device 10 is stopped, and the rotation of the rotor 142 of the centrifuge 20 is stopped (step S106).

  With the above, the plasma collection process is completed, and the process proceeds to the blood component return process. That is, blood component return processing A is performed (step S107). In this blood component return step, the blood components remaining in the blood component collection circuit 2 (mainly the blood storage space 146 of the rotor 142) (remaining blood components) are returned to the donor.

  As shown in FIG. 4, in this blood component return processing A, first, the first flow path opening / closing means 51 is opened, the second flow path opening / closing means 52 is closed, and the first liquid delivery pump 11 is turned on. It operates (reverses) at a predetermined rotational speed (preferably about 30 to 100 mL / min, for example, 45 mL / min).

  As a result, blood cells in the blood storage space 146 of the rotor 142 begin to be returned to the donor via the first line 21 and the blood collection needle 29. Further, the air stored in the storage bag 26 is transferred into the blood storage space 146 of the centrifuge 20 via the second line 22 and the fourth line 24, and returns (step S401).

  During this time, the control unit 55 sequentially detects the blood return amount from the start of the blood component return process A by counting the number of rotations of the first liquid feeding pump 11, and this blood return amount is preset. It is determined whether or not the predetermined amount has been reached (step S402).

  When the amount of blood returned from the start of blood component return processing A reaches a predetermined amount, the first flow path opening / closing means 51 is closed and the second flow path opening / closing means 52 is opened. As a result, a predetermined amount (set amount) of plasma in the plasma collection bag 25 is transferred into the blood storage space 146 of the centrifuge 20 via the second line 22 and returned (step S403).

  At this time, blood components other than plasma (blood cells) adhering in the flow path between the blood storage space 146 and the plasma collection bag 25 (mainly near the outlet 144 of the blood storage space 146) due to the flowing plasma. Is captured and transported into the blood reservoir space 146. Thereby, in the plasma collection process of the next cycle, mixing of blood components (blood cells) other than plasma into the plasma collection bag 25 is prevented.

  Based on the change in the weight of the plasma collection bag 25 detected by the weight sensor 16, the control unit 55 determines whether or not the amount of plasma transferred from the plasma collection bag 25 to the centrifuge 20 has reached a set amount. Judgment is made (step S404). The set amount of plasma at this time is not particularly limited, but is preferably about 1 to 120 mL, and more preferably about 5 to 50 mL.

  When the amount of plasma transferred reaches the set amount in step S404, the process proceeds to step S405.

  In step S405, the first flow path opening / closing means 51 is opened, and the second flow path opening / closing means 52 is closed. Thereby, the air stored in the storage bag 26 is transferred into the blood storage space 146 of the centrifuge 20 via the second line 22 and the fourth line 24 and returned.

  When the blood return to the donor is continued in this way, the air in the first line 21 reaches the first bubble sensor 17 and is detected by the first bubble sensor 17 (step S406). After the air is detected by the first bubble sensor 17, the control unit 55 further rotates the first liquid feeding pump 11 a predetermined number of times (step S407), and then stops the first liquid feeding pump 11.

  The number of rotations of the first liquid delivery pump 11 in step S407 is the number of revolutions until the air in the first line 21 reaches the first bubble sensor 17 and the blood cells in the chamber 21d disappear and are almost empty. It is preset so as to correspond to the amount of liquid.

Thus, the blood component return process A ends (step S408).
When the blood component return processing A in the first cycle is completed, the process proceeds to the plasma collection step of the plasma collection operation in the second cycle shown in FIG.

  The plasma collection operation from the third cycle to the cycle before the final cycle is the same as the plasma collection operation in the second cycle described below.

  As shown in FIG. 5, in the plasma collection process of the plasma collection operation of the second cycle, blood collection is first started (step S201), similarly to the plasma collection process of the plasma collection operation of the first cycle described above. The air in the blood component collection circuit 2 such as in the blood storage space 146 of the centrifuge 20 is transferred into the storage bag 26 and stored via the line 22 and the fourth line 24 (step S202).

  In the blood storage space 146, the plasma remaining in the blood storage space 146 and the blood newly introduced into the blood storage space 146 through the first line 21 are once mixed, and the mixed blood is The plasma layer 131 and the blood cell layer 132 are separated from the inside by centrifugal force.

  When plasma flowing out from the blood storage space 146 of the centrifuge 20 is detected by the third bubble sensor 27 (step S203), the storage of the air in the blood storage space 146 into the storage bag 26 is completed.

  Next, a predetermined amount (set amount) of plasma in the blood storage space 146 of the centrifuge 20 is transferred and stored in the storage bag 26 via the second line 22 and the fourth line 24, and is removed. An operation is performed (step S204).

  Specifically, the first channel opening / closing means 51 is opened and the second channel opening / closing means 52 is closed. As a result, a predetermined amount of plasma in the blood storage space 146 of the centrifuge 20 is transferred and stored in the storage bag 26 via the second line 22 and the fourth line 24. At this time, blood components other than plasma (blood cells) adhering in the flow path between the blood storage space 146 and the storage bag 26 (mainly near the outlet 144 of the blood storage space 146) are caused by the flowing plasma. Is captured and transferred into the storage bag 26. By performing this initial flow removal operation, in the plasma collection process of this cycle, mixing of blood components (blood cells) other than plasma into the plasma collection bag 25 is prevented.

  When the amount of plasma transferred from the blood storage space 146 to the storage bag 26 reaches the set amount (step S205), the process proceeds to step S206.

  Whether or not the amount of plasma transferred from the blood storage space 146 into the storage bag 26 has reached the set amount is determined by counting the number of rotations of the first liquid feeding pump 11. That is, when the count value of the number of rotations of the first liquid delivery pump 11 becomes a value corresponding to a preset amount of plasma that is set in advance, it is determined that the amount of plasma has reached the set amount.

  The set amount of the plasma amount is not particularly limited, but is preferably about 1 to 120 mL, and more preferably about 15 to 75 mL.

  Next, similarly to the plasma collection step of the plasma collection operation in the first cycle described above, the first flow path opening / closing means 51 is closed, the second flow path opening / closing means 52 is opened, and the second line 22 is connected. Then, the plasma in the blood storage space 146 of the centrifugal separator 20 is transferred to the plasma collection bag 25 and collected (step S206), and the end of the plasma collection is determined (step S207), and the above (1) to (4) ) Is detected and the plasma collection is terminated, the operation of the first liquid feeding pump 11 and the second liquid feeding pump 12 is stopped and the operation of the centrifuge drive device 10 is stopped. The rotation is stopped and the rotation of the rotor 142 of the centrifuge 20 is stopped (step S208).

  With the above, the plasma collection process is completed, and the process proceeds to the blood component return process. That is, blood component return processing B shown in FIG. 6 is performed (step S209).

  Hereinafter, the blood component return process B will be described, but the difference from the blood component return process A in the first cycle described above will be mainly described, and description of similar matters may be omitted.

  As shown in FIG. 6, in the blood component return processing B, first, the plasma stored in the storage bag 26 is stored in the centrifuge 20 via the second line 22 and the fourth line 24. It is transferred back into the space 146 (step S501).

  Specifically, the first flow path opening / closing means 51 is opened, the second flow path opening / closing means 52 is closed, and the first liquid feed pump 11 is operated (reversely rotated) at a predetermined rotational speed. Thereby, blood cells in the blood storage space 146 of the rotor 142 begin to be returned to the donor via the first line 21 and the blood collection needle 29, and plasma stored in the storage bag 26 is returned to the second line 22 and the fourth line. Is transferred into the blood storage space 146 of the centrifuge 20 through the line 24 (see FIG. 7).

  At this time, the amount ZmL of plasma returned from the storage bag 26 into the blood storage space 146 is substantially the same as the amount of plasma transferred from the blood storage space 146 into the storage bag 26 in step S204 described above.

  When air is detected by the third bubble sensor 27 because the storage bag 26 has become empty (step S502), blood is returned until the blood return reaches a predetermined amount (step S503). Next, a predetermined amount (set amount) of plasma in the plasma collection bag 25 is transferred into the blood storage space 146 of the centrifuge 20 via the second line 22 and returned (step S504).

  In step S504, the first flow path opening / closing means 51 is closed, and the second flow path opening / closing means 52 is opened. As a result, a predetermined amount of plasma in the plasma collection bag 25 is transferred into the blood storage space 146 of the centrifuge 20 via the second line 22 and returned (see FIG. 8). At this time, blood components other than plasma (blood cells) adhering to the flow path between the blood storage space 146 and the plasma collection bag 25 are captured and transferred to the blood storage space 146 by the flowing plasma. . Thereby, in the plasma collection process of the next cycle, mixing of blood components (blood cells) other than plasma into the plasma collection bag 25 is prevented.

  Since the specific gravity of plasma is lighter than that of blood cells, as shown in FIG. It is maintained in a state of being divided into two layers.

  When the amount of plasma transferred from the plasma collection bag 25 to the centrifuge 20 reaches the set amount (step S505), the process proceeds to step S506.

  In step S506, the first flow path opening / closing means 51 is opened, the second flow path opening / closing means 52 is closed, and stored in the storage bag 26 via the second line 22 and the fourth line 24. The air in the centrifuge 20 is transferred into the blood storage space 146 and returned.

  As a result, as shown in FIG. 9, blood cells in the blood storage space 146 are further returned to the donor via the first line 21 and the blood collection needle 29, and the liquid level 134 in the blood storage space 146 further decreases.

  Here, the control unit 55 of the present embodiment includes a counter that counts the number of rotations of the first liquid feeding pump 11. Since the number of rotations of the first liquid feeding pump 11 and the amount of liquid feeding are in a correspondence relationship, the control unit 55 counts the number of rotations of the first liquid feeding pump 11 to thereby determine the number of rotations in the first line 21. It can function as a liquid feeding amount detecting means for detecting the liquid feeding amount of blood or blood components.

  The control unit 55 sequentially detects the blood return amount from the start of the blood component return process B by counting the number of rotations of the first liquid delivery pump 11, and this blood return amount is set in advance. It is determined whether or not a fixed amount (XmL) has been reached (step S507).

  When the blood return amount from the start of the blood component return process B reaches a predetermined amount (XmL), the centrifuge drive device 10 is operated to start rotating the rotor 142 of the centrifuge 20 (step S508). FIG. 10). The rotation speed of the rotor 142 is preferably about 3000 to 6000 rpm, more preferably about 4500 to 5700 rpm.

  The timing for starting the rotation of the rotor 142 at this time is such that almost all blood cells disappear from the blood storage space 146 and blood mainly composed of plasma remains in the blood storage space 146 as shown in FIG. The predetermined amount XmL is set to a value that can realize such timing.

  The predetermined amount XmL can be determined, for example, as follows in the design stage. First, the amount of blood plasma YmL remaining in the blood storage space 142 when almost all blood cells disappear from the blood storage space 146 is obtained by experiments. In step S501, the amount of plasma returned from the storage bag 26 to the blood storage space 146 is set to ZmL, and in step S503, the amount of plasma returned from the plasma collection bag 25 to the blood storage space 146 is set to WmL. When the volume of the blood storage space 146 is VmL, the predetermined amount XmL can be determined by the equation X = V−Y + Z + W.

  When the rotation of the rotor 142 is started in step S508, the plasma in the blood storage space 146 is accumulated on the outer peripheral side in the blood storage space 146 by centrifugal force as shown in FIG. As a result, the air transferred from the storage bag 26 to the blood storage space 146 with the plasma remaining in the blood storage space 146 flows into the first line 21 through the tube body 141 and the inlet 143. Therefore, with the plasma remaining in the blood storage space 146, only the blood cells in the first line 21 can be pushed away with air toward the blood collection needle 29 and returned to the donor.

  If blood return to the donor is continued in this way, the air in the first line 21 reaches the first bubble sensor 17 and is detected by the first bubble sensor 17 (step S509). After the air is detected by the first bubble sensor 17, the control unit 55 further rotates the first liquid feeding pump 11 a predetermined number of times (step S510), and then stops the first liquid feeding pump 11.

  As described above, as shown in FIG. 12, almost all blood cells in the blood component collection circuit 2 are returned to the donor, and the blood component return process B is completed (step S511). At this time, plasma remains in the blood storage space 146 as shown in FIG.

  Thus, in the blood component return process B, as shown in FIGS. 7 and 8, both the plasma in the storage bag 26 and a part of the plasma in the plasma collection bag 25 are contained in the blood storage space 146. Returned. Thereby, in the cycle immediately before the second cycle to the final cycle, a predetermined amount of plasma remains in the blood storage space 146 in the state shown in FIG.

  When the blood component return processing B is completed, if this cycle is two or more cycles before the final cycle, the process proceeds to the plasma collection step of the plasma collection operation shown in FIG. In the case of the previous cycle, the process proceeds to the plasma collection step of the plasma collection operation in the final cycle shown in FIG.

  Next, in the plasma collecting process of the plasma collecting operation of the final cycle shown in FIG. 13, blood collection is first started (step S301), as in the plasma collecting process of the plasma collecting operation of the second cycle described above. Via the line 22 and the fourth line 24, the air in the blood component collection circuit 2 such as in the blood storage space 146 of the centrifuge 20 is transferred and stored in the storage bag 26 (step S302), and the third When the blood bubble sensor 27 detects plasma flowing out from the blood storage space 146 of the centrifuge 20 (step S303), the storage of the air in the blood storage space 146 into the storage bag 26 is completed.

  Next, similarly to the plasma collection step of the plasma collection operation in the second cycle described above, a predetermined amount (set amount) of plasma in the blood storage space 146 of the centrifuge 20 is supplied to the second line 22 and the fourth line 24. Then, it is transferred to and stored in the storage bag 26 (step S304).

  Thereby, blood components (blood cells) other than plasma adhering in the flow path between the blood storage space 146 and the storage bag 26 (mainly near the outlet 144 of the blood storage space 146) due to the flowing plasma. Is captured and transferred into the storage bag 26. Thereby, in the plasma collection process of this cycle, mixing of blood components (blood cells) other than plasma into the plasma collection bag 25 is prevented.

  When the amount of plasma transferred from the blood storage space 146 into the storage bag 26 reaches a set amount, as in the plasma collection step of the plasma collection operation in the second cycle described above (step S305), the first flow path The opening / closing means 51 is closed, the second flow path opening / closing means 52 is opened, and the plasma in the blood storage space 146 of the centrifuge 20 is transferred into the plasma collection bag 25 via the second line 22. Sample is collected (step S306), the end of plasma collection is determined (step S307), and if any one of the above (1) to (4) is detected and plasma collection is terminated, the first liquid delivery While stopping operation | movement of the pump 11 and the 2nd liquid feeding pump 12, operation | movement of the centrifuge drive device 10 is stopped, and rotation of the rotor 142 of the centrifuge 20 is stopped (step S308).

  With the above, the plasma collection process is completed, and the process proceeds to the blood component return process. That is, the blood component return process C shown in FIG. 14 is performed (step S309).

  Hereinafter, the blood component return process C will be described, but the difference from the blood component return process A described above will be mainly described, and the description of the same matters may be omitted.

  In this blood component return processing C, first, the first flow path opening / closing means 51 is opened, the second flow path opening / closing means 52 is closed, and the first liquid feed pump 11 is operated at a predetermined rotational speed ( Reverse). Thus, blood cells in the blood storage space 146 of the rotor 142 begin to be returned to the donor via the first line 21 and the blood collection needle 29, and the plasma and air stored in the storage bag 26 are returned to the second line 22. Then, the blood is transferred into the blood storage space 146 of the centrifuge 20 via the fourth line 24 and returns (step S601).

  When air is detected by the third bubble sensor 27 (step S602), blood is returned until the blood return reaches a predetermined amount (step S603).

  Next, the first flow path opening / closing means 51 is closed, and the second flow path opening / closing means 52 is opened. Thereby, a predetermined amount of plasma in the plasma collection bag 25 is transferred into the blood storage space 146 of the centrifuge 20 via the second line 22 and returned. At this time, blood components other than plasma (blood cells) adhering to the flow path between the blood storage space 146 and the plasma collection bag 25 are captured and transferred to the blood storage space 146 by the flowing plasma. . Thereby, even when an additional cycle is performed after the final cycle, contamination of blood components (blood cells) other than plasma into the plasma collection bag 25 is prevented in the plasma collection step of the additional cycle.

  When the amount of plasma transferred from the plasma collection bag 25 to the blood storage space 146 reaches the set amount (step S605), the process proceeds to step S606.

  In step S606, the first flow path opening / closing means 51 is opened and the second flow path opening / closing means 52 is closed, so that the inside of the storage bag 26 is passed through the second line 22 and the fourth line 24. The air stored in the centrifuge 20 is transferred into the blood storage space 146 of the centrifuge 20 and returned. When air is detected by the second bubble sensor 18 (step S607), the return of the blood component is terminated (step S608), and the program is terminated.

  Thereby, almost all the remaining blood components in the blood component collection circuit 2 are returned to the donor, and the plasma collection operation is completed. Thus, in the blood component return process C in the final cycle, unlike the blood component return process B, all the blood components in the blood storage space 146 are returned without leaving plasma in the blood storage space 146. The rotor 142 is stopped from start to finish without rotating 142 (step S508).

  In the blood component return processing C, steps S603 to S606 are steps provided in consideration of performing an additional cycle, and thus can be omitted.

  As described above, according to the blood component collection device 1, the plasma in the blood storage space 146 of the centrifuge 20 is collected in the plasma collection bag 25 in the plasma collection step of each cycle after the second cycle. Prior to this, a predetermined amount of plasma in the blood storage space 146 of the centrifuge 20 is transferred and stored in the storage bag 26 via the second line 22 and the fourth line 24. As a result, blood components other than plasma (mainly blood cells, in particular, adhering to the inside of the flow path between the blood storage space 146 and the storage bag 26 (mainly near the outlet 144 of the blood storage space 146) due to the flowing plasma. White blood cells) are captured and transported into the blood reservoir space 146. As a result, it is possible to prevent blood components (blood cells, particularly white blood cells) other than plasma from entering the plasma collection bag 25.

  Further, in the blood component return step of each cycle except the final cycle, a predetermined amount of plasma in the plasma collection bag 25 is transferred to the blood storage space 146 of the centrifuge 20 via the second line 22 and returned. In such a case, blood other than plasma adhering in the flow path between the blood storage space 146 and the plasma collection bag 25 (mainly near the outlet 144 of the blood storage space 146) due to the flowing plasma. Components (blood cells, particularly white blood cells) are captured and transported into the blood reservoir space 146. Thereby, contamination of blood components other than plasma (blood cells, particularly white blood cells) into the plasma collection bag 25 can be more reliably prevented.

  Further, by rotating the rotor 142 of the centrifuge 20 from the middle of the blood component return process, the plasma returned from the storage bag 26 or the plasma collection bag 25 into the blood storage space 146 is not returned to the donor, but the blood storage space 146. The blood component return process is terminated with the plasma remaining in the interior. Therefore, compared with the case where all the blood components in the blood storage space 146 are returned to the donor, the plasma collection efficiency can be improved. Moreover, the time required for the blood component return step can be shortened. Thereby, since the required time as a whole can also be shortened, the number of donors that can collect blood per day with one blood component collection device 1 can be increased, and the efficiency can be improved.

The operation method of the blood component collection device of the present invention has been described based on the illustrated embodiments. However, the present invention is not limited to this, and the configuration of each part of the blood component collection device is the same. Any structure having a function can be substituted. Moreover, other arbitrary components may be added to the blood component collection device .

  In the present invention, the blood component collection device is not limited to the case where the blood component collection device is applied to a plasma collection device that collects plasma, and can be applied to, for example, a blood component collection device that collects platelets, plasma, and red blood cells.

It is a top view (partly including a block diagram) which shows the blood component collection device in embodiment of the operation method of the blood component collection device of this invention. FIG. 2 is a partially broken cross-sectional view showing a state where a centrifuge drive device is mounted on a centrifuge provided in the blood component collection device shown in FIG. 1. It is a flowchart which shows the control action of the control part in a 1st cycle. It is a flowchart which shows the control operation (subroutine) of the control part in the blood component return process A in FIG. It is a flowchart which shows the control action of the control part in the cycle before the 2nd cycle-the last cycle. It is a flowchart which shows the control operation (subroutine) of the control part in the blood component return process B in FIG. It is a schematic diagram for explaining the state in the blood component collection circuit in the blood component return processing B step by step. It is a schematic diagram for explaining the state in the blood component collection circuit in the blood component return processing B step by step. It is a schematic diagram for explaining the state in the blood component collection circuit in the blood component return processing B step by step. It is a schematic diagram for explaining the state in the blood component collection circuit in the blood component return processing B step by step. It is a schematic diagram for explaining the state in the blood component collection circuit in the blood component return processing B step by step. It is a schematic diagram for explaining the state in the blood component collection circuit in the blood component return processing B step by step. It is a flowchart which shows the control action of the control part in the last cycle. It is a flowchart which shows the control operation (subroutine) of the control part in the blood component return process C in FIG.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Blood component collection apparatus 2 Blood component collection circuit 10 Centrifugal separator drive device 11 1st liquid feeding pump 12 2nd liquid feeding pump 14 Turbidity sensor 15 Optical sensor 16 Weight sensor 17 1st bubble sensor 18 2nd Bubble sensor 20 Centrifuge 21 First line (blood collection / return line)
22 Second line 23 Third line 24 Fourth line 25 Plasma collection bag (blood component collection bag)
251 hole portion 26 storage bag 261 hole portion 27 third bubble sensor 28 fourth bubble sensor 29 blood collection needle 32 sub bag 321 hole portion 33 cassette housing 51 first channel opening / closing means 52 second channel opening / closing means 55 Control unit 61 Branch connector 62 Tube 63 Filter 64 Disinfection filter 65, 66 Clemme 91 First opening 92 Second opening 131 Plasma layer 132 Blood cell layer 133 Interface 134 Liquid surface 141 Tube 142 Rotor 143 Inlet 144 Flow Outlet 145 Upper part 146 Blood storage space 151 Housing 152 Leg part 153 Motor 154 Rotating shaft 155 Fixing base 156 Bolt 157 Spacer 158 Mounting member 21a Blood collecting needle side first line 21b Centrifuge side first line 21c Branch connector for connection 21d Chamber 2 1g 1st pump tube 21h tube 21i filter 23a 2nd pump tube 23c bubble removal chamber 23d anticoagulant welder connection needle 32a tube S101 to S107 step S201 to S209 step S301 to S309 step S401 to S408 step S501 S511 step S601 to S608 step

Claims (6)

Blood collection means for collecting blood from a donor,
A centrifuge that includes a rotor having a blood storage space therein, and centrifuges blood collected by the blood collection means by rotation of the rotor in the blood storage space;
A blood collection / return line connecting the blood collection means and the centrifuge;
A blood component collection bag that communicates with the blood storage space and collects a predetermined blood component separated by the centrifuge;
A blood component collection circuit comprising a storage bag communicating with the blood storage space ;
A liquid feeding means provided on the blood collection / return line;
Channel opening and closing means provided on the blood collection / return line;
Control means for controlling the liquid feeding means and the flow path opening and closing means ;
A plurality of cycles of blood component collection operations comprising: a blood component collection step of centrifuging blood collected from a donor and collecting the predetermined blood component; and a blood component return step of returning the remaining blood components to the donor A method of operating a blood component collection device to perform,
In the blood component collection step in the blood component collection operation of each cycle after the second cycle , the control means controls the predetermined amount before collecting the predetermined blood component in the blood component collection bag. Performing an initial flow removal operation of transferring the predetermined blood component of the storage bag to the storage bag,
In the blood component return step in the blood component collection operation of each cycle from the second cycle to the last cycle, the predetermined blood component in the storage bag is returned to the blood storage space, and initially the rotor By continuing the return of the remaining blood components while stopping the rotation and rotating the rotor from the middle so that the predetermined blood components are accumulated on the outer peripheral side in the blood storage space by centrifugal force The method for operating a blood component collecting apparatus, wherein the blood component return step is terminated in a state where the predetermined blood component remains in the blood storage space. The blood according to claim 1, wherein the control means returns a part of the predetermined blood component in the blood component collection bag to the blood storage space in the blood component return step of the blood component collection operation by the control. Method of operating the component collection device. In the blood component collection step, the air that was initially in the blood storage space as the blood collected by the blood collection means is transferred to the blood storage space through the blood collection / return line by the control of the control means. Is transferred into the storage bag,
In the blood component return step, the air in the storage bag is returned to the blood storage space as the remaining blood components in the blood storage space are returned to the blood donor under the control of the control means. A method for operating the blood component collection device according to 1 or 2.   When the rotor rotates in the blood component returning step when the rotor is rotated from the middle and the predetermined blood component is accumulated on the outer peripheral side in the blood storage space, the air in the blood storage space is the blood collection The method for operating a blood component collection device according to any one of claims 1 to 3, wherein the blood component collection device can flow into the blood return line. The blood component according to any one of claims 1 to 4, wherein the control means does not rotate the rotor partway through the control in the blood component return step of the blood component collection operation in the final cycle. How to operate the sampling device. In the blood component return step when the rotor is rotated halfway through the control, the control means detects the blood return amount from the start of the blood component return step by the liquid feed amount detection means, and is detected. The method for operating a blood component collection device according to any one of claims 1 to 5, wherein rotation of the rotor is started when the amount of returned blood reaches a predetermined amount.

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