JP4854194B2 - Lamella structure regenerative agent and skin external preparation - Google Patents

Description

The present invention relates to an external preparation having an excellent moisturizing effect, preventing skin dryness and aging, and moisturizing the skin. More specifically, the present invention relates to skin moisture by regenerating the lamellar structure of intercellular lipids in the horny layer. The present invention relates to an external preparation for skin that can enhance the holding function.

The stratum corneum intercellular lipid exists in a lamellar structure between the stratum corneum cells and is deeply involved in the stratum corneum water retention mechanism. Therefore, it is indispensable that the lamella structure is normally formed in order to maintain the moisture of the skin and the flexibility of the stratum corneum (Non-patent Document 1 edited by the Japan Cosmetic Science Society, “functional cosmetics”. , 1st edition, DISC Corporation, issued on July 12, 1991, the second printing, p.
235-252).
There has been known an invention that aims to improve rough skin by applying a skin external preparation having a lamellar structure and to normalize the skin lamellar structure by permeating the skin external preparation. For example, a topical skin preparation that forms a lamellar layer containing a sterol derivative (Patent Document 1, Japanese Patent Laid-Open No. 05-294989), a lipid bilayer and an amino acid, a higher fatty acid, or a higher alcohol. JP-A-07-285827), an external preparation for skin containing a lipid bilayer and an aliphatic alcohol (Patent Document 3 JP-A-2003-081809), betaine, S-adenosylmethionine, and lamellar structure Cosmetic composition to be formed (Patent Document 4 Special Table 2004-501069), composition containing neutral lipid and bilayer component (Patent Document 5 Special Table 2000-513706), lipid component of stratum corneum A composition containing ceramide or the like intended to be supplied to a lipid lamella and hydroxycarboxylic acid (Patent Document 6) 57283 JP), oil-in-water emulsion with lamellar liquid crystal phase (Japanese Patent Document 7 JP-T 2001-518886) and the like.
Further, a composition containing collagen tripeptide, 2-pyrrolidone-5-carboxylic acid and lactic acid is known as a wet composition that promotes and stabilizes lamellar structure formation (Patent Document 8: Japanese Patent Application Laid-Open No. 2001-064154). Publication).
The present inventors believe that if the lamellar structure of stratum corneum intercellular lipids can be regenerated, the moisture retention function of the stratum corneum can be recovered, moisture can be given to the skin, and rough skin and sensitive skin can be eliminated. In addition, intensive research on the regeneration of such a lamellar structure has been continued, and an external preparation for skin containing a hydrophilic polymer having a function of regenerating the lamellar structure in skin stratum corneum cells is disclosed (Patent Document 9) 2003-238341).

JP 05-294989 A Japanese Patent Application Laid-Open No. 07-285827 JP 2003-081809 A Japanese translation of PCT publication No. 2004-501069 Special Table 2000-513706 Japanese Patent Laid-Open No. 06-157283 Special table 2001-518886 JP 2001-064154 A JP 2003-238341 A Edited by Japan Cosmetic Science Society, “Functional Cosmetics”, 1st Edition, DISQUES Inc., July 12, 1991, 2nd edition, p. 235-252

Between stratum corneum cells, lipids between stratum corneum cells having a lamellar structure are present, and it has been revealed that they are deeply involved in a barrier function that prevents moisture retention and entry of foreign substances from the outside. In other words, so-called “sensitive skin” that is prone to trouble with skin irritation or irritation to the skin, such lamellar structures between stratum corneum cells disappear or are damaged. It is confirmed that the lamellar structure damage of the stratum corneum intercellular lipid leads to a decrease in the stratum corneum moisture retention function, and causes dry skin and rough skin.

Therefore, if the lamellar structure of stratum corneum intercellular lipids can be regenerated, it is considered that the stratum corneum moisture retention function can be recovered, moisture can be given to the skin, and rough skin and sensitive skin can be eliminated. The present inventors have continued research and development on the study on the regeneration of such a lamellar structure. It has been found that the decrease in the stratum corneum water content due to damage to the lamella structure as described above cannot be recovered by glycerin, which is a typical moisturizing agent.

The present invention has been made by paying attention to the above-mentioned function of the lamellar structure of the stratum corneum intercellular lipid in the skin surface layer part, and is a lamella of stratum corneum intercellular lipid that can enhance the moisture retention function of the skin It aims at providing the agent which reproduces | regenerates a structure, and a new skin external preparation.

The main configuration of the present invention is as follows.
(1) A lamellar structure regenerating agent for stratum corneum cells of skin , comprising glucose and raffinose, wherein the mixing ratio of glucose and raffinose is 4: 1 to 1: 4 in terms of concentration ratio.
(2) The lamellar structure regenerating agent for stratum corneum cells of the skin according to (1), wherein the blending amount of glucose is 0.1 to 10% by mass and the blending amount of raffinose is 0.1 to 10% by mass.
(3) A skin external preparation containing the lamellar structure regenerating agent in the horny layer cells of the skin according to (1) or (2) .

By using glucose and oligosaccharides together, a lamellar structure regenerating agent for stratum corneum cells with excellent usability and high practicality was realized. Excellent for practical use as a skin external preparation and cosmetic. The lamellar structure regeneration action becomes prominent by selecting a specific blending amount, blending ratio or specific oligosaccharide.

The skin protects the body from various external stimuli and has a barrier function that prevents evaporation of moisture inside. The lamellar structure composed of stratum corneum intercellular lipids is largely involved in the barrier function in the stratum corneum, and a method for easily observing the lamellar structure from the exfoliated stratum corneum has been established. Proved that
It is known that lamellar structure (maltase cross image) can be observed from a mixture of ceramide, cholesterol and other components that make up stratum corneum intercellular lipids and water, and this method is effective for the barrier function of the stratum corneum. Some important ingredients have been found.
Therefore, for the purpose of searching for effective ingredients for dry skin with reduced barrier function, we focused on highly moisturizing sugars and useful ingredients using an evaluation method using artificial stratum corneum intercellular lipids. Explored. As a result, an excellent effect on glucose and raffinose was found, and the barrier function recovery effect could be demonstrated in a human test. Furthermore, we have obtained useful results for the barrier effect on cultured epidermal cells in a dry environment.

Hereinafter, embodiments of the present invention will be described in detail.
The external preparation for skin according to the present invention comprises glucose and / or raffinose, or glucose and oligosaccharides.
The blending amount of glucose or raffinose is preferably 0.1 to 10% by mass, more preferably 0.5 to 5% by mass. If it is 0.1% by mass or less, the effect of regenerating the lamellar structure of stratum corneum intercellular lipid is not sufficient, and if it is 10% by mass or more, the stickiness increases and the usability is poor.

As a compounding quantity of oligosaccharide, it is more preferable to set it as the range of 0.1-10 mass%, and also 0.5-5 mass%. If it is 0.1% by mass or less, the effect of regenerating the lamellar structure of stratum corneum intercellular lipid is not sufficient, and if it is 10% by mass or more, the stickiness increases and the usability is poor.

The mixing ratio of glucose and oligosaccharide is preferably a molar concentration ratio of 13: 1 to 1: 1.2, and the regeneration effect of the lamellar structure of the stratum corneum intercellular lipid is synergistically increased.

In addition, when glucose is used alone, the moisturizing feeling becomes high, but “stickiness” becomes strong and the feeling of use is bad. When oligosaccharides are used alone, the moisturizing feeling is not so high, but it is refreshing. By using glucose and oligosaccharides, particularly glucose and melibiose, trehalose, and raffinose in combination, a feeling of use with high moisturizing feeling and less “stickiness” can be obtained.

Examples of oligosaccharides include melibiose, trehalose, raffinose, sucrose,
Examples include maltose, lactose, cellobiose, gentianose, stachyose, cyclodextrin and the like. Oligosaccharides can be used alone or in combination of two or more. Among oligosaccharides, melibiose, trehalose, and raffinose are particularly effective in regenerating the lamellar structure of stratum corneum intercellular lipids.
The dosage form of the external preparation for skin according to the present invention is not particularly limited, and can be applied to external preparations for skin such as facial cleansers, lotions, emulsions, creams, packs and the like.

In the upper skin external preparation according to the present invention, in addition to the hydrophilic polymer, a moisturizer, amino acid, vitamins, hydrocarbon, higher fatty acid, esters, silicon, anionic surfactant, cationic surfactant, Amphoteric surfactants, nonionic surfactants, pH adjusters, water and the like can be appropriately blended as necessary. Each of these components may be used alone or in combination of two or more.

Examples of the moisturizing agent include polyethylene glycol, propylene glycol, glycerin, 1,3-butylene glycol, sorbitol, maltitol, chondroitin sulfate, collagen, sodium lactate, dl-pyrrolidone carboxylate, yokoinine extract, brown sugar extract, soybean Examples include lecithin.

As amino acids, for example, neutral amino acids such as glycine, alanine, valine, leucine, isoleucine, serine, threonine, tryptophan, cystine, methionine, proline, hydroxyproline, glutamine, asparagine, acidic amino acids such as aspartic acid, glutamic acid, and arginine, Examples include basic amino acids such as histidine, lysine, and hydroxylysine.

Examples of vitamins include vitamins A, B1, B2, B6, C and derivatives thereof, E and derivatives thereof, pantothenic acid and derivatives thereof, biotin and the like.

Examples of the hydrocarbon include liquid paraffin, paraffin, squalane and petrolatum.

Examples of higher fatty acids include lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, isostearic acid, linoleic acid, linolenic acid, eicosapentaenoic acid (EPA), Examples include docosahexaenoic acid (DHA).

Esters include isopropyl myristate, cetyl octanoate, octyldodecyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, decyl oleate, hexyl decyl dimethyloctanoate, cetyl lactate, myristyl lactate, Lanolin acetate, isocetyl stearate, isocetyl isostearate, cholesteryl 2-hydroxystearylate, ethylene glycol di-2-ethylhexylate, dipentaerythritol fatty acid ester, monoisostearic acid N-alkylglycol, dicapric acid neopentylglycol, malic acid diacid Isostearyl,
Glycerin di-2-heptylundecanoate, glyceryl tri-2-ethylhexylate,
Trimethylolpropane triisostearate, cetyl 2-ethylhexanoate, 2-
Ethylhexyl palmitate, glyceryl trimyristate, tri-2-heptylundecanoic acid glyceride, castor oil fatty acid methyl ester, 2-hexyldecyl myristate, 2-hexyldecyl palmitate, 2-hexyldecyl adipate, diisopropyl sebacate, succinate Examples include 2-ethylhexyl acid and triethyl citrate.

Examples of silicon include chain polysiloxanes such as dimethylpolysiloxane and methylphenylpolysiloxane, cyclic polysiloxanes such as decamethylcyclopentasiloxane and dodecamethylcyclohexasiloxane, and silicon resins forming a three-dimensional network structure. Is mentioned.

Examples of the anionic surfactant include acyl amino acid salts such as acyl glutamate, fatty acid soaps such as sodium laurate and sodium palmitate, higher alkyl sulfates such as sodium lauryl sulfate and potassium lauryl sulfate, and POE lauryl sulfate trioxide. Examples include ethanolamine, alkyl ether sulfates such as POE sodium lauryl sulfate, and N-acyl sarcosine salts such as sodium lauroyl sarcosine.

Examples of the cationic surfactant include alkyltrimethylammonium salts such as stearyltrimethylammonium chloride and lauryltrimethylammonium chloride, distearyldimethylammonium dialkyldimethylammonium chloride, and poly (N, N′-chloride).
And alkylpyridinium salts such as dimethyl-3,5-methylenepiperidinium chloride, cetylpyridinium chloride, alkyl quaternary ammonium salts, POE alkylamines, alkylamine salts, polyamine fatty acid derivatives, amyl alcohol fatty acid derivatives, and the like.

Examples of amphoteric surfactants include 2-undecyl-N, N, N- (hydroxyethylcarboxymethyl) -2-imidazoline sodium, 2-cocoyl-2-imidazolinium hydroxide-1-carboxyethyloxy 2 Betaine amphoteric surfactants such as sodium salts such as imidazoline-based amphoteric surfactants, 2-heptadecyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, lauryldimethylaminoacetic acid betaine, alkylbetaines, amide betaines, sulfobetaines Examples include activators.

Examples of the nonionic surfactant include sorbitan monooleate, sorbitan monoisostearate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan sesquioleate, sorbitan trioleate, penta-2- Sorbitan fatty acid esters such as diglycerol sorbitan ethylhexylate, diglycerol sorbitan tetra-2-ethylhexylate, glyceryl monostearate,
α, α'-oleic acid pyroglutamate glycerin, glycerin polyglycerin fatty acids such as monostearic acid glycerin malic acid, propylene glycol fatty acid esters such as propylene glycol monostearate, hardened castor oil derivative, glycerin alkyl ether, POE-sorbitan Monostearate, POE-sorbitan monooelate, PO
POE sorbitan fatty acid esters such as E-sorbitan tetraoleate, POE sorbite fatty acid esters such as POE-sorbite monolaurate, POE-sorbite monooleate, POE-sorbite pentaoleate, POE-sorbite monostearate, POE -POE glycerol fatty acid esters such as glycerol monostearate, POE-glycerol monoisostearate, POE-glycerol triisostearate,
POE monooleate, POE distearate, POE monodiolate, POE fatty acid esters such as ethylene glycol stearate, POE castor oil, POE hydrogenated castor oil, POE hydrogenated castor oil monoisostearate, POE hydrogenated castor oil triisostearate, POE cured Castor oil monopyroglutamic acid monoisostearic acid diester, POE
POE castor oil hardened castor oil derivatives such as hardened castor oil maleic acid.

Examples of the pH adjuster include buffers such as lactic acid-sodium lactate, citric acid-sodium citrate, and phosphorous-sodium dihydrogen phosphate.

[Test 1]
1. Test method for lamellar structure regeneration test using saccharide alone Artificial intercellular lipids were prepared, and an aqueous sugar solution was added to regenerate the lamellar structure. The image observed with a polarizing microscope was binarized to determine the area of the lamella structure, and the regeneration effect for each type of sugar was evaluated.

Artificial intercellular lipid A Ceramide 0.12g
0.72 g of water
B Cholesterol 0.08g
Stearic acid 0.08g
A and B were each dissolved at 80 ° C., then mixed, cooled to 10 ° C. and irradiated with ultrasonic waves for 10 minutes. It melt | dissolved and mixed again at 80 degreeC, it cooled to 10 degreeC, and irradiated the ultrasonic wave for 10 minutes.

Sugar aqueous solution
Various 0.25M sugar aqueous solutions were prepared. As a mechanism to stabilize the lamella structure, it was assumed that sugars were oriented at the lamella interface, and the lamellar regeneration effect was assumed to depend on the number of molecules and compared at the same molar concentration.

Formation of Lamella Structure 10 mg of artificial intercellular lipid was collected, 20 μl of an aqueous sugar solution was added, heated in an 80 ° C. water bath, and mixed well. Thereafter, a lamellar structure was generated by repeating ultrasonic waves for 10 minutes at 10 ° C. and heating for 10 minutes in a water bath at 80 ° C. four times.

Polarizing microscope observation Polarizing microscope: γ = 530 nm, BX50, OLYMPUS, magnification: 100 times, observation range: approx. 220 μm × 164 μm, image analysis software: WinROOF, manufactured by Mitani Corporation

Result The polarizing microscope observation image was analyzed and the area of the lamellar liquid crystal was measured. Area 1500μm ²
The above was evaluated as ◎, 1000 μm ² or more and less than 1500 μm ² as ◯, and less than 1000 μm ² as Δ, and the lamellar liquid crystal regeneration effect was evaluated in three stages. The results are shown in Table 1. In addition, polarizing microscope images of ribose and raffinose are shown in FIGS.

Among the monosaccharides, glucose has a large effect on regenerating the lamellar structure. Glucose has a larger e-OH value (value obtained by proportional distribution from the proportions of various conformations present in the solution than the number of equatorial OH groups) compared to other monosaccharides in the table. When compared with sucrose, melibiose, trehalose (disaccharide), and raffinose (trisaccharide), the lamella structure regeneration effect is arranged in order from the largest in order of raffinose, trehalose, melibiose, and sucrose. I'm doing it. Therefore, the larger the value of e-OH, the greater the lamellar structure regeneration effect. Since α-cyclodextrin and γ-cyclodextrin have a large molecular weight, the proportion of OH that can be oriented in a lamellar structure out of OH in the molecule decreases. Therefore, at the same molar concentration, the lamellar structure regeneration effect is small as compared with disaccharides and trisaccharides despite the large value of e-OH.

[Test 2] 2. The lamellar structure regeneration test of sugar The evaluation method using artificial intercellular lipid was examined.
Test method An artificial intercellular lipid was prepared, and an aqueous sugar solution was added thereto to regenerate the lamellar structure. The image observed with a polarizing microscope was binarized to determine the area of the lamella structure, and the regeneration effect for each type of sugar was evaluated.

Artificial intercellular lipid A Ceramide (ceramide II, III, VI) 0.12g (each 0.04g)
Cholesterol sulfate 0.02g
0.25 g of water
B Cholesterol 0.08g
Stearic acid 0.08g

A and B were each melted at 80 ° C., then mixed and sonicated at 10 ° C. for 10 minutes. The mixture was melted and mixed again at 80 ° C., and sonicated at 10 ° C. for 10 minutes.

As a mechanism for stabilizing the conditioned lamellar structure of the aqueous sugar solution, we assumed that the orientation of the e-OH group of the constituent monosaccharide contributed to the stabilization of the lamellar interface, and compared at the same weight percent concentration.

Generation of lamellar structure
A 20% sugar aqueous solution was prepared, and 15 μl was added to 60 mg of artificial intercellular lipid to make the final sugar concentration 1%. Heat in an 80 ° C water bath and mix well. Then, a lamellar structure was generated by repeating ultrasonic waves for 10 minutes at 10 ° C. and heating for 10 minutes in a water bath at 80 ° C. twice.

Polarizing microscope observation Polarizing microscope: γ = 530 nm, BX50, OLYMPUS, magnification: 200 times, observation range: about 110 μm × 82 μm, image analysis software: WinROOF, manufactured by Mitani Corporation

Evaluation Method 2 mg of artificial intercellular lipid was collected, spread evenly with a cover glass, and observed with a polarizing microscope. The area of the lamella structure was measured by analyzing 30 μm × 30 μm (10 images of about 110 μm × 82 μm were selected by avoiding foreign substances other than the maltase cross image) in the polarizing microscope observation image. The average value of 10 areas was defined as the lamellar structure area.
Furthermore, the lamella structure regeneration promotion rate was calculated | required using the following formula | equation.

Lamella structure regeneration promotion rate (%) = 100 × [(Lamellar structure area of sugar) − (Lamellar structure area without added sugar)] / [Lamellar structure area without added sugar]

The results are shown in Table 7.

When comparison was made by the number of e-OH groups per constituent monosaccharide, the lamellar structure regeneration effect did not depend on the number of e-OH groups per constituent monosaccharide in polysaccharides higher than disaccharides. This is considered to indicate that the influence on the lamella interface is different depending on the steric structure of sugars in polysaccharides of disaccharides or higher.
The formation of lamellar structures is promoted for all sugars except ribose compared to no sugar addition (FIG. 4), and the effect is best for raffinose (FIG. 5), followed by glucose.

[Test 3]
3. Test method for moisture content change in dry environment
A 5% aqueous solution was prepared using raffinose or glucose dried overnight in a dryer set at 65 ° C. The air weight of a dish for cell culture (35 mm ² × 10 mm, FALCON) was measured, 2 ml of an aqueous solution was added, the weight was measured again, and the weight difference was taken as the weight of the aqueous solution. The dish was placed in a relatively stable environment at room temperature of 50 to 55 ° C. and relative humidity of 28 to 38%, and the weight was measured after 1, 2, 3, and 4 hours.

Results The weight of raffinose and aqueous glucose solution was reduced to half in 3 hours (FIG. 6). There was no statistically significant difference between the two.

[Test 4]
4). Test method for inhibition of cell lethality of epidermal keratinocytes in a dry environment 4% raffinose or glucose was added to cultured human epidermal keratinocytes and cultured at 37 ° C.
After 30 minutes of culture, the culture supernatant was removed, gas phase exposure (0 to 60 minutes) was performed, and the cells were cultured at 37 ° C. to be in a dry environment. The cell viability was determined by MTT assay, and the cell killing inhibitory effect was evaluated. The results are shown in FIG.

Results When no sugar was added, the cell viability of epidermal keratinocytes decreased with increasing drying time. The cells cultured in the raffinose-added medium had a cell viability of about 85% and glucose of about 60%, and raffinose inhibited the damage of epidermal keratinocytes due to drying.

[Test 5]
5. Barrier function recovery test method for dry and rough skin Nine male and female volunteers with healthy skin were subjected to acetone / ether treatment (1: 1, 10 minutes x 2 times) to create an artificial dry rough skin condition on the inner forearm. . For rough skin, raffinose-free and raffinose 3% lotion was used for 6 days. Before continuous use, 3 days, 6 days later, the stratum corneum moisture (SKICON-200EX, IBS) and transdermal moisture transpiration (2ch moisture transpiration monitor AS-TW, Asahi Biomed) were measured to determine the skin barrier function. Recovery was assessed. In addition, the stratum corneum moisture amount was a value after rough skin treatment, and the evaluation of the transdermal moisture transpiration amount was an amount of change relative to the value before treatment.

Results Skin rejuvenation was promoted by applying lotion on dry skin. The stratum corneum moisture content increased to the same extent for both of the two types of lotions (Fig. 8 stratum corneum moisture content), and the transdermal moisture transpiration rate was compared with the unconcentrated lotion when the raffinose 3% lotion was used continuously. Thus, the recovery was significantly promoted (FIG. 9 change in transdermal water transpiration amount).

[Test 6]
6). Test method of raffinose stimulation defense test 14 men and women with sensitive skin were subjected to 10-day raffinose-free and raffinose-containing lotion for 10 days, and then their irritation sensitivity was compared. In the stimulus sensitivity test, a non-woven fabric soaked with 1% lactic acid was applied to the left and right for 5 minutes, and the subjects scored the stimulus intensity in 7 stages every 30 seconds (stimulus score), and the total scores were compared.

Results The difference in the total stimulation score before and after the continuous use of raffinose 3% lotion was significantly lower than that of the non-formation lotion (FIG. 10), and the average values were 11.5 and 16.6. These results suggested the usefulness of raffinose in sensitive skin with low defense against external stimuli.

[Evaluation of Tests 2-6]
Sugar, which is mainly used as a moisturizing agent in cosmetics, has been studied for its water retention ability, but there are few inventions that have confirmed the action related to the barrier function of sugar. The following were found and confirmed by these tests 2-6.
As a result of evaluating the contribution of sugars to the formation process of lamellar structure composed of artificial stratum corneum intercellular lipid and water, it was found that raffinose has an excellent regeneration effect on lamellar structure. When lotion containing raffinose was used continuously for dry and rough skin, recovery of the stratum corneum moisture content was comparable to that without raffinose. On the other hand, the recovery of transdermal moisture transpiration has a significant effect on raffinose blended products, suggesting that raffinose has a high contribution to the recovery effect of stratum corneum barrier function among sugars.
In addition, the cell viability of epidermal cells in a dry environment is higher than that of glucose, and raffinose is useful not only in the stratum corneum but also in the protective effect on the dry environment in the epidermis. Suggests. This seems to support the protective effect against external stimuli when using lotion containing raffinose.
From the above points, it was confirmed that raffinose is a useful component particularly in skin having a reduced barrier function due to drying.

[Test 7]
7). Test method for lamellar structure regeneration test using glucose and raffinose in combination The same test as in Example 1 was performed. Glucose and raffinose were added as sugars to be added, and the mixing ratio was changed for evaluation.

Result The polarizing microscope observation image was analyzed and the area of the lamellar liquid crystal was measured. The area is 2000μm ²
The above-mentioned results were evaluated as follows: ◎, 1500 μm ² or more and less than 2000 μm ² was evaluated as ◯, and less than 1500 μm ² was evaluated as Δ. The results are shown in Table 2.

Glucose and raffinose at a concentration ratio of 4: 1 to 1: 4 and a molar ratio of 13: 1 to 1: 1
. It was found that by combining within the range of 2, the lamellar structure regeneration effect becomes synergistically greater than when used in combination outside the range.

Method for testing moisturizing effect by two-week continuous test Formulation Example 1 containing glucose and raffinose at a weight ratio of 5: 3 and Comparative Example 1 containing glucose but not raffinose were used for two weeks. . Further, Formulation Example 2 containing glucose and raffinose at a weight ratio of 1: 4 and Comparative Example 2 containing glucose but not raffinose were used continuously for two weeks. In the continuous test (90 women) using Prescription Example 1 and Comparative Example 1, texture evaluation before and after use and questionnaire after use were carried out. In the continuous test using Formulation Example 2 and Comparative Example 2 (62 women), only a questionnaire after use was conducted.
The formulation is shown in Table 3.

Evaluation of texture before and after use in the 2-week continuous test for each half-face of Prescription Example 1 and Comparative Example 1 Replicas of the left and right cheeks of the subject before and after the 2-week continuous test were collected with a skin cast manufactured by Yamada Cosmetic Co., Ltd.
The LED light source is irradiated onto the replica at an elevation angle of 30 °, and the shadow image is displayed on the actual microscope (SZ-PT, OLY
Observed by MPUS) and acquired a digital image. The image was binarized in black and white, subjected to edge processing, and the number of parts surrounded by skin grooves (the number of textures) was counted.
Table 4 shows the average number of textures before and after the 2-week continuous test. FIG. 3 shows the increase in texture after the 2-week continuous test. Although the number of textures increased in both Formulation Example 1 and Comparative Example 1, the increase in the number of textures was greater in Formulation Example 1. Formulation Example 1 formulated with a combination of glucose and raffinose has a greater effect of regenerating the lamellar structure in the stratum corneum cells of the skin than Comparative Example 1 formulated with glucose alone, and as a result of improved skin condition, the number of textures increased. Conceivable.

Questionnaire results after continuous use of Prescription Example 1 and Comparative Example 1 for half a week for two weeks A questionnaire was conducted according to the following evaluation criteria.

(Skin familiarity)
1 point: Bad 2 points: Slightly bad 3 points: Neither can be said 4 points: Slightly good 5 points: Good (moist feeling)
1 point: not moist 2 points: not very moist 3 points: can't say either 4 points: slightly moist 5 points: moist
1 point: not fluffy 2 points: not fluffy 3 points: cannot say 4 points: slightly fluffy 5 points: fluffy (durability of moist feeling)
1 point: Not sustained 2 points: Not very persistent 3 points: Neither can be said 4 points: Slightly sustained 5 points: Sustained (Comprehensive evaluation)
1 point: Bad 2 points: Slightly bad 3 points: Neither can be said 4 points: Slightly good 5 points: Good

Table 5 shows the questionnaire results.

Formulation Example 1 formulated with a combination of glucose and raffinose has all of “Family Skin”, “Moistness”, “Fluffy”, “Durability of Moistness”, and “Comprehensive Evaluation” over Comparative Example 1 with only glucose Was excellent.

Results of questionnaire after prescription example 2 and comparative example 2 were used continuously for half a week for two weeks After prescription example 2 and comparative example 2 were used for half a week for two weeks according to the same evaluation criteria as prescription example 1 and comparative example 1 A questionnaire was conducted.
Table 6 shows the questionnaire results.

Formulation Example 2 formulated with a combination of glucose and raffinose has all of “Family Skin”, “Moistness”, “Fluffy”, “Durability of Moistness”, and “Comprehensive Evaluation” over Comparative Example 2 with only glucose Was excellent.

A formulation example as a skin lotion is shown below.

Formulation Example 3 Lotion (Ingredient) Blending amount (mass%)
Glucose 2.0
Trehalose 2.0
Glycerin 5.0
1,3-butylene glycol 10.0
Sodium carboxymethyldextrin 0.1
Dipotassium glycyrrhizinate 0.1
Purified water residue

A prescription example as an emulsion is shown below.

Formulation Example 4 Emulsion (component) Blending amount (% by mass)
Glucose 4.0
Melibiose 1.0
Glycerin 8.0
1,3-butylene glycol 5.0
Squalane 3.0
Olive oil 1.0
Cetyl octanoate 5.0
Stearic acid 0.3
Polydimethylsiloxane 0.5
Polysorbate 60 0.5
Glyceryl monostearate 1.0
Carboxyvinyl polymer 0.1
Potassium hydroxide 0.01
Purified water residue

Polarized light micrograph showing lamellar liquid crystal regeneration when an aqueous ribose solution is added Polarized light micrograph showing lamellar liquid crystal regeneration when raffinose aqueous solution is added Graph showing increase in texture after 2-week continuous test of Formulation Example 1 and Comparative Example 1 Polarized light micrograph showing the effect of promoting the formation of lamellar structure (A: no addition of sugar) Polarized light micrograph showing lamella structure formation promoting effect (B: 1% raffinose added) Graph showing moisture content change in dry environment Graph showing the effect of inhibiting cell killing in a dry environment Graph showing water content of stratum corneum Graph showing the amount of transdermal moisture transpiration Graph comparing stimulus scores after continuous use of model lotion

Claims (3)

A lamellar structure regenerating agent for stratum corneum cells of skin , comprising glucose and raffinose, wherein the mixing ratio of glucose and raffinose is 4: 1 to 1: 4 in a concentration ratio.   The lamellar structure regenerating agent for stratum corneum cells of skin according to claim 1, wherein the amount of glucose is 0.1 to 10% by mass and the amount of raffinose is 0.1 to 10% by mass. The skin external preparation containing the lamellar structure regeneration agent in the stratum corneum cell of the skin of Claim 1 or 2 .