JP4880233B2 - Anti-dermatological agent and external preparation for skin containing the same - Google Patents

Description

  The present invention relates to an anti-dermatological agent that suppresses or ameliorates skin damage caused by exposure to ultraviolet rays, and an external preparation for skin containing the same. More specifically, an anti-dermatological agent comprising a Compositae Chrysanthemum genus plant extract as an active ingredient, and a skin extracellular matrix component resulting from exposure to ultraviolet rays, comprising the anti-dermatological agent of the Asteraceae Chrysanthemum genus plant extract. The present invention relates to a skin external preparation useful as a cosmetic or pharmaceutical agent that suppresses or improves at least one of abnormal accumulation, collagen cross-linking formation, wrinkle formation, skin thickening, and skin hardening.

Long-term exposure to ultraviolet rays (e.g. sunlight) causes skin to become irritated by chemical mediators, cytokines, etc., and skin disorders such as wrinkles, talmi, skin thickening, skin sclerosis, skin cancer, solar elastic fibrosis, etc. (See Non-Patent Document 1). In particular, thickening of the epidermis and dermis results in a decrease in skin elasticity and moisture retention, which is considered to be one of the causes of skin aging. Therefore, in order to prevent these obstacles conventionally, an external preparation containing an ultraviolet absorber (see Patent Document 1 and Non-Patent Document 2) and an ultraviolet scattering agent (see Non-Patent Document 3), that is, an emulsion, cream, lotion, Cosmetic liquids, foundations, ointments, cataplasms, patches, etc. are used.
In addition, retinoic acid (see Non-Patent Document 4), anti-inflammatory drug (see Non-Patent Document 5) in order to prevent or improve skin wrinkles, tarmi, elasticity and reduced elasticity caused by exposure to ultraviolet rays, etc. ), Buckwheat extract, birch extract, sage extract, roman chamomile extract, etc. (see Patent Document 2), Melissa extract (see Patent Document 3), and also active in inhibiting abnormal accumulation of extracellular matrix components, skin thickening, wrinkles, etc. Formulation of type vitamin D ₃ (see Non-Patent Document 6) has been reported.

However, for example, a topical skin preparation containing retinoic acid has the effect of improving the wrinkle by proliferating collagen in the upper layer of the dermis, but is not effective in suppressing the generation of wrinkles. In addition, when the application site is exposed to ultraviolet rays, there are problems such as inducing skin cancer. The active vitamin D ₃ have a side effect problems such as calcium metabolism. Even in the topical skin preparations containing other UV absorbers, UV scattering agents, anti-inflammatory agents, plant extracts, etc., these are not effective in suppressing or improving wrinkle formation, skin thickening, etc. When these additives are blended at a high concentration, the usability of the preparation may be impaired, and problems such as deterioration at high temperatures and over time may occur.

JP 09-268194 A Japanese Patent Application Laid-Open No. 08-109122 JP 09-241148 A Tsutomu Sugawara and Keiichi Nozu, “Solar UV and Health,” Yukabo (1998) p. 2-100 Nicholas J. Rowe / Nadim A. Germ, “Sunscreens and Dermatology”, Fragrance Journal, published May 20, 1993, p. 195-262 “Fragrance Journal”, Fragrance Journal, July 2002 16-27, 33-38 Lorraine H. Kligman, “Effects of all-trans-retinoic acid on the dermis of hairless rice,” Journal of the American Academy of Dermatology, 1988. 15, no. 4, Part. 2, October, P.M. 779-785 Bissett DL, et al. Author, “Photoprotective effect of topical anti-inflammatory agents against ultraradiation radiation-induced chronic skin damage in the hair 90.” 7, p. 153-158 Koshiishi I, et al. "1,25-dihydroxyvitamin D3 presents the conversion of adipose tissue into fibrosstic in inspired toporic UV iradiation.", Toxol. 173, P.I. 99-104

  Accordingly, an anti-dermatological agent that effectively suppresses or ameliorates at least one skin disorder such as abnormal accumulation of extracellular matrix components, collagen cross-linking formation, wrinkle formation, skin thickening, and skin hardening caused by ultraviolet exposure, and There has been a demand for the development of an external preparation for skin containing this without adversely affecting the feeling of use and dosage form.

  In order to achieve the above object, the present inventors have focused on plants already used in pharmaceuticals, cosmetic raw materials, folk medicines, etc., which have already been confirmed to be safe, and as a result of intensive research, Chrysanthemum plant extract suppresses the abnormal accumulation of extracellular matrix components caused by UV exposure, cross-linking of collagen and elastin, and further the development of at least one skin disorder such as wrinkle formation, skin thickening, skin hardening, etc. The present invention was completed by obtaining new knowledge that the effect of improvement was excellent.

  That is, the present invention relates to an anti-dermatological agent that suppresses and improves skin damage caused by exposure to ultraviolet rays, comprising an extract of Asteraceae plant extract as an active ingredient, and the Asteraceae plant belongs to Chrysantheum Makino Matsum. etNakai), Kikutanigiku (chrysanthemum boreale Makino), Shimakangiku (chrysanthemum indicum L.), chrysanthemum pacificum (chrysanthemum pacificum), garland chrysanthemum (chrysanthemum coronarium), France chrysanthemum (chrysanthemum Leucanthemum L.), and feverfew (chrysanthemum parthenium Peres.) (hereinafter, Each plant Latin notation scientific name is related to the anti-skin-disorder agent is at least one or more selected from the group consisting of a drawing). Further, the present invention relates to an anti-dermatological agent in which the skin damage caused by ultraviolet exposure is at least one of abnormal accumulation of skin extracellular matrix components, collagen cross-linking formation, wrinkle formation, skin thickening, and skin hardening. Still further, the present invention relates to an external preparation for skin containing the anti-dermatological agent as an active ingredient and suppressing or improving the above-mentioned skin damage, particularly to an external preparation for preventing aging.

  The Asteraceae plant extract according to the present invention suppresses abnormal accumulation of skin extracellular matrix components and collagen cross-linking formation, wrinkle formation, skin thickening, and skin hardening caused by ultraviolet exposure. Or it is an anti-dermatological agent highly effective in improvement. The topical skin preparation containing the anti-dermatological agent as an active ingredient effectively suppresses or ameliorates the skin disorder caused by exposure to ultraviolet rays. Therefore, a cosmetic or quasi-drug that prevents skin aging. Etc., which can be used advantageously.

Hereinafter, the present invention will be described in detail.
The Asteraceae plant extract used in the present invention is an abnormal accumulation of extracellular matrix components caused by exposure to ultraviolet rays, particularly abnormal production of collagen, collagen cross-linking, wrinkle formation, skin thickening, skin hardening, etc. It suppresses or improves at least one skin disorder. There are no particular limitations on the Asteraceae genus plant extract used in the present invention, but among these, Ryunogi, Kikutaniku, Shimakangi, Rhizome, Sungiku, French Giku, and Feverfew are preferred from the viewpoint of effects.

  The Chrysanthemum plant extract used in the present invention is not particularly limited in its preparation method, and any of whole plant or root, stem, stem, bark, bud, leaf, flower, fruit, seed, etc. It is possible to extract from one or more places, and after subjecting these to appropriate treatment such as drying, chopping, pressing, or fermentation, extraction with a solvent at low temperature or room temperature to warming can be mentioned.

  Examples of the extraction solvent include water, lower monohydric alcohols (methyl alcohol, ethyl alcohol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (glycerin, propylene glycol, 1, 3 -Butylene glycol, etc.), lower alkyl esters (ethyl acetate, etc.), hydrocarbons (benzene, hexane, pentane, etc.), ketones (acetone, methyl ethyl ketone, etc.), ethers (diethyl ether, tetrahydrofuran, dipropyl ether, etc.), acetonitrile 1 type, or 2 or more types can be used. Particularly preferred extraction solvents include water or water-ethyl alcohol mixed solvents.

  Further, the extract may be any of a molecular weight fraction, a solvent fraction, a product purified by various resin treatments (ion exchange resin, adsorbent, etc.), and a freeze-dried product.

  As a specific production method, for example, a dried plant product is pulverized, and about 10-fold mass concentration of hydrous ethyl alcohol or 1,3-butylene glycol is used for extraction or reflux extraction at room temperature for 1 to 5 days. After 2 hours or after 1 hour of hot water extraction, it is filtered and the extract is further purified by molecular weight fractionation, solvent fractionation, various resin treatments (ion exchange resin, adsorbent, etc.), etc. There are methods such as lyophilization to obtain a solid. In a water-ethyl alcohol solvent, ethyl alcohol is distilled off and then lyophilized to obtain a solid content.

  The form of the anti-dermatological agent of the present invention is not particularly limited as long as it is contained as a Chrysanthemum plant extract, and can be used in any form such as liquid, paste, cream, gel, and spray drying. It is also possible to use it as a powder after being dried by, for example.

  The external preparation for skin of the present invention is characterized by containing an anti-dermatopathic agent of the Asteraceae plant extract. The content of the anti-dermatological agent in the external preparation for skin of the present invention is not particularly limited, but is 0.0001 to 10% by mass (hereinafter, simply referred to as “%”) as a dry solid content in the external preparation for skin. And preferably 0.001 to 5%. Within this range, abnormal accumulation of extracellular matrix components due to UV exposure, especially abnormal production of collagen and collagen cross-linking, as well as the development of at least one skin disorder such as wrinkle formation, skin thickening, skin hardening, etc. It is excellent in the effect of suppressing or improving the symptom thereof, and good in terms of stability over time can be obtained.

  In addition to the above essential components, the external preparation for skin containing the anti-dermatological agent of the present invention includes various components usually used in cosmetics, quasi-drugs, external medicines, that is, water, alcohols, oils, interfaces. Active agents, thickeners, powders, chelating agents, pH adjusters, whitening agents, anti-inflammatory agents, antioxidants, moisturizers, bactericides, blood circulation promoters and other medicinal agents, extracts derived from animals, plants and microorganisms In addition, an ultraviolet absorber, an ultraviolet scattering agent, a fragrance and the like can be appropriately added depending on the purpose within a range not impairing the effects of the present invention.

  Examples of the external preparation for skin containing the anti-dermatological agent of the present invention include cosmetics, quasi-drugs, pharmaceuticals, etc., and the dosage form is an aqueous dosage form, an oil-based dosage form, an emulsifier type, a powder dosage form, It can mix | blend with any dosage forms, such as a solid dosage form. For example, as cosmetics, it can be used in lotions, milky lotions, creams, cosmetic liquids, packs, bath salt ointments, gels, foundations, powders, lip balms, lipsticks, sunscreen products, and the like.

  Next, although the manufacture example of a plant extract and an Example are given and this invention is demonstrated further in detail, this invention is not restrict | limited at all.

Production examples 1 to 11 of the Asteraceae genus plant extract, and production examples 12 of birch bark extract whose anti-wrinkle action and anti-aging action have already been reported as samples for comparative examples Indicated.
[Production Example 1]
1,000 mL of a 25 vol% aqueous ethanol solution was added to 100 g of a crushed dried product of Ryunogi flower, and reflux extraction was performed for 2 hours. Centrifugation, pressure filtration, and membrane separation were used to collect a sample having a molecular weight of 5,000 or less to obtain 600 mL of an extract, which was concentrated under reduced pressure using an evaporator. After distilling off ethanol, this solution was freeze-dried. 7.4 g was obtained as a solid.

[Production Example 2]
1,000 mL of a 25 vol% aqueous ethanol solution was added to 100 g of the crushed dry matter of Kikutaniku flowers, and reflux extraction was performed for 2 hours. Centrifugation, pressure filtration, and membrane separation were used to collect a product having a molecular weight of 5,000 or less, and 650 mL of an extract was obtained and concentrated under reduced pressure using an evaporator. After distilling off ethanol, this solution was freeze-dried. 6.9 g was obtained as a solid.

[Production Example 3]
Purified water (1,000 mL) was added to 100 g of crushed dried citrus flowers, and hot water extraction was performed for 1 hour. Centrifugation, pressure filtration, and membrane separation were used to collect one having a molecular weight of 5,000 or less to obtain 620 mL of an extract. After concentration under reduced pressure using an evaporator, this solution was lyophilized to obtain a solid as 9. 3 g was obtained.

[Production Example 4]
1,000 mL of 25 vol% ethanol aqueous solution was added to 100 g of a dried product of dried sea oyster leaves, and reflux extraction was performed for 2 hours. Centrifugation, pressure filtration, and membrane separation were used to collect a sample having a molecular weight of 5,000 or less, 700 mL of an extract was obtained, concentrated under reduced pressure using an evaporator, ethanol was distilled off, and this solution was freeze-dried. 5.7 g was obtained as a solid.

[Production Example 5]
1,000 mL of a 25 vol% ethanol aqueous solution was added to 100 g of a crushed dried syringa flower, and reflux extraction was performed for 2 hours. Centrifugation, pressure filtration, and membrane separation were used to collect a product having a molecular weight of 5,000 or less, to obtain 610 mL of an extract, which was concentrated under reduced pressure with an evaporator, ethanol was distilled off, and this solution was freeze-dried. 7.4 g was obtained as a solid.

[Production Example 6]
1,000 mL of a 25 vol% aqueous ethanol solution was added to 100 g of a crushed dried dried daisy flower, and reflux extraction was performed for 2 hours. Centrifugation, pressure filtration, and membrane separation were used to collect a sample having a molecular weight of 5,000 or less, 700 mL of an extract was obtained, concentrated under reduced pressure using an evaporator, ethanol was distilled off, and this solution was freeze-dried. 5.4 g was obtained as a solid.

[Production Example 7]
Purified water (1,000 mL) was added to 100 g of dried fractured feverfew, and hot water extraction was performed for 1 hour. This was subjected to centrifugal separation and pressure filtration, and membrane separation was used to collect one having a molecular weight of 5,000 or less to obtain 670 mL of an extract, which was freeze-dried to obtain 7.5 g as a solid.

[Production Example 8]
Purified water (1,000 mL) was added to 100 g of crushed dried synghi leaf, and hot water extraction was performed for 1 hour. Centrifugation, pressure filtration, and membrane separation were used to collect a product having a molecular weight of 5,000 or less to obtain 760 mL of an extract, which was concentrated under reduced pressure using an evaporator. 9 g was obtained.

[Production Example 9]
1,000 mL of a 25 vol% aqueous ethanol solution was added to 100 g of a crushed dried product of feverfew, and reflux extraction was performed for 2 hours. Centrifugation, pressure filtration, and membrane separation were used to collect a sample having a molecular weight of 5,000 or less, and 660 mL of an extract was obtained and concentrated under reduced pressure using an evaporator. After distilling off ethanol, this solution was freeze-dried. 6.6 g was obtained as a solid.

[Production Example 10]
1,000 mL of a 25 vol% ethanol aqueous solution was added to 100 g of the crushed dried citrus leaves, and reflux extraction was performed for 2 hours. Centrifugation, pressure filtration, and membrane separation were used to collect a sample having a molecular weight of 5,000 or less, and 580 mL of an extract was obtained and concentrated under reduced pressure using an evaporator. After distilling off ethanol, this solution was freeze-dried. 5.5 g was obtained as a solid.

[Production Example 11]
1,000 mL of a 25 vol% aqueous ethanol solution was added to 100 g of a crushed dry matter of whole sea anemone, and reflux extraction was performed for 2 hours. Centrifugation, pressure filtration, and membrane separation were used to collect a sample having a molecular weight of 5,000 or less, and 660 mL of an extract was obtained and concentrated under reduced pressure using an evaporator. After distilling off ethanol, this solution was freeze-dried. 8.2 g were obtained as a solid.

[Production Example 12]
1,000 mL of 25 vol% ethanol aqueous solution was added to 100 g of a crushed dried birch bark, and reflux extraction was performed for 2 hours. Centrifugation, pressure filtration, and membrane separation were used to collect a sample having a molecular weight of 5,000 or less, and 660 mL of an extract was obtained and concentrated under reduced pressure using an evaporator. After distilling off ethanol, this solution was freeze-dried. 7.4 g was obtained as a solid.

Example 1: Skin damage test by hairless mouse UV irradiation Anti-skin damage preparation was prepared by the following preparation method, skin thickening by UV irradiation, wrinkle formation, skin hardening, abnormal accumulation of skin extracellular matrix components (total hydroxyproline amount Dermatan sulfate amount) and collagen crosslinking (pepsin resistant hydroxyproline) were evaluated.

[Preparation of sample (anti-dermatological preparation)]
A chrysanthemum plant extract of Production Examples 1 to 11 and a birch bark extract of Production Example 12 were dissolved in a base (polyethylene glycol 1000: ethyl alcohol = 1: 1), and a sample was prepared to a solid content concentration of 5%. It was used for the skin evaluation test by hairless Mousus ultraviolet irradiation.

[Sample application method and UV irradiation method]
One group consists of 8 animals, and 0.1 g of the above sample is applied to the back of hairless mice (10 weeks old) 90 minutes before UV irradiation, and a certain amount of UV light (Toshiba FL20S / BLB lamp) is applied for 2 hours a day (5 times). / Week) Irradiation was performed for 20 weeks (total irradiation amount: 720 J / cm ² ), and skin thickening, wrinkle formation, skin hardening, abnormal accumulation of skin extracellular matrix components, and inhibition of collagen cross-linking formation were examined.
In addition, the ultraviolet absorption spectrum of these samples was measured, and it was confirmed that these did not affect the evaluation test.

[Evaluation method]
(Skin thickening suppression effect)
The thickness of the skin before ultraviolet irradiation and 20 weeks after ultraviolet irradiation was measured using a dial thickness gauge (OZAK.MFG.CO.LTD.). The results were evaluated by the average value of the skin thickness of 8 animals and the rate of increase after 20 weeks.

(Wrinkle formation inhibitory effect)
About wrinkle formation 20 weeks after ultraviolet irradiation, the wrinkle grade was determined based on the “photoskin aging grade” shown in Table 4 below. In addition, the result was expressed and evaluated as an average value of 8 scores.

(Skin hardening inhibitory effect)
The central part of the back of the hairless mouse skin was picked, and the skin requiring 5 seconds or more for restoration was regarded as skin hardening, and the expression rate in 8 mice was evaluated.

[Abnormal accumulation of extracellular matrix components; total hydroxyproline content, dermatan sulfate content]
(Total hydroxyproline; collagen determination method)
Hydroxyproline in the skin was measured and the amount of abnormal collagen accumulation was evaluated.
For the determination of hydroxyproline, first, a frozen section (20 microns) of hairless mouse dorsal skin was prepared, and the skin section was heat-treated on a slide glass, and then 0.05% alkaline protease (actinase E; manufactured by Kaken Pharmaceutical) (500 tyrosinase) (Unit / mL) was enzymatically decomposed (40 ° C.-2 hours) and solubilized. Thereafter, the sample was vacuum-sealed, hydrolyzed with 6N hydrochloric acid (145 ° C. for 4 hours), and then hydroxyproline was colored by the Woessner method and measured.

(Dermatan sulfate determination method)
Like collagen, abnormal accumulation of dermatan sulfate, an extracellular matrix component, is one of the indicators of skin aging caused by ultraviolet irradiation, and the degree of skin aging was evaluated by measuring this component. After formalin fixation of the hairless mouse dorsal skin, a 6-micron skin section was prepared, and the skin section was enzyme-enzymed with chondroitinase ABC (0.5 units / mL) and collagenase (500 mandel units / mL) on a slide glass. After decomposition (37 ° C-2 hours) and solubilization, the sample is separated using an HPLC apparatus by the post-column method under the following conditions, mixed with the reaction reagent 1 and the reaction reagent 2, and then at 110 ° C-2 minutes The reaction was carried out in the interior to obtain a fluorescent derivative, which was measured with a fluorescence detector.

(HPLC conditions)
HPLC column: DOCOSIL (4.6id × 150mm: manufactured by Senshu Scientific Co., Ltd.)
Fluorescence detection: Ex. 346 nm, Em. 410nm
Mobile phase: 8.5% acetonitrile-1 mM tetra n-butylammonium hydrogensulfate Flow rate: 1.5 mL / min Column temperature: 60 ° C.
Reaction reagent 1: 0.3 M NaOH (0.25 mL / min)
Reaction reagent 2: 0.25% 2-cyanoacetamide

(Collagen cross-linking: Pepsin resistant hydroxyproline quantitative method)
The degree of collagen cross-linking in the skin was evaluated based on the difficulty of degradation by acid protease (pepsin; manufactured by Nacalai Tesque).
First, a frozen section (20 microns) of the hairless mouse dorsal skin prepared by the above-described collagen quantification method was enzymatically degraded with 0.01% pepsin (366 units / mL) on a slide glass (5 ° C.-64 hours) to allow pepsin. The dissolved collagen was washed with water and removed. Thereafter, pepsin-resistant collagen was heated by the same method as described above, and then enzymatically decomposed using actinase E and solubilized. Next, the solution was sealed in a vacuum, hydrolyzed with 6N hydrochloric acid (145 ° C. for 4 hours), and then developed with hydroxyproline by Woessener method to measure pepsin resistant hydroxyproline.

(Pepsin resistant hydroxyproline (%) calculation method)
The content of pepsin resistant hydroxyproline relative to total hydroxyproline in the skin was calculated and used as an index for collagen cross-linking formation.
Pepsin resistant hydroxyproline content (%) = (pepsin resistant hydroxyproline / total hydroxyproline) × 100

Tables 1 to 3 show the evaluation results of skin thickening, wrinkle formation, skin hardening, abnormal accumulation of extracellular matrix components (total hydroxyproline amount, dermatan sulfate amount), and collagen cross-linking (pepsin resistant hydroxyproline amount, content), respectively. Show.

(Wrinkle grade (light skin aging grade) criteria)

As is clear from Tables 1 to 3, the Chrysanthemum plant extracts of Production Examples 1 to 11 of the present invention are compared with the birch bark extract that is a comparative example, all of which are marked skin thickening, skin hardening, wrinkle formation, It was extremely excellent in the effect of suppressing abnormal accumulation of extracellular matrix components and collagen crosslinking.

Example 2 Cream (ingredient) (%)
(1) Monostearic acid polyethylene glycol (40E.O.) 2.0
(2) Self-emulsifying glyceryl monostearate 5.0
(3) Stearic acid 5.0
(4) Behenyl alcohol 1.0
(5) Liquid paraffin 10.0
(6) Glyceryl trioctanoate 10.0
(7) Ryunogi flower extract * 1 0.5
(8) Kikutaniku flower extract * 2 2.0
(9) Glycerin 5.0
(10) Preservative appropriate amount (11) perfume appropriate amount (12) remaining amount of purified water * 1 Production Example 1
* 2 Production Example 2

(Manufacturing method)
A. Ingredients (1)-(6) are mixed and heated to keep at 70 ° C.
B. Ingredients (9), (10) and (12) are mixed and heated to keep at 70 ° C.
C. B is added to A and emulsified.
D. Components (7), (8) and (11) were added to C, and then cooled to obtain a cream.

  Example 2 is stable without discoloration and odor and has a high smooth emollient effect when applied to the skin, and continuously applied to suppress or improve skin disorders such as wrinkle formation, skin thickening, and skin hardening. It was excellent in effect.

Example 3 lotion (ingredient) (%)
(1) Glycerin 10.0
(2) 1,3-butylene glycol 6.0
(3) Citric acid 0.1
(4) Sodium citrate 0.3
(5) Shimakangi Flower Extract * 1 0.5
(6) Sea anemone flower extract * 2 0.5
(7) Purified water remaining amount (8) polyoxyethylene (60E.O.) hydrogenated castor oil 0.5
(9) Ethyl alcohol 8.0
(10) Preservative appropriate amount (11) Fragrance appropriate amount * 1 Production Example 3
* 2 Production Example 4

(Manufacturing method)
A. Components (1) to (7) are mixed and dissolved.
B. Components (8) to (11) are mixed and dissolved.
C. A and B were mixed and made uniform to obtain a skin lotion.

  Example 3 is stable without discoloration, odor, precipitation, and the like, and has a fresh moisturizing feeling when applied to the skin. By applying continuously, skin damage such as wrinkle formation, skin thickening, and skin hardening is suppressed or It was effective in improving.

Example 4 Latex (component) (%)
(1) Sorbitan monostearate 0.3
(2) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.1
(3) Lipophilic glyceryl monostearate 0.2
(4) Stearic acid 0.5
(5) Cetanol 0.5
(6) Squalane 3.0
(7) Liquid paraffin 4.0
(8) Glyceryl tri-2-ethylhexanoate 2.0
(9) Dimethylpolysiloxane 1.0
(10) Hydrogenated soybean phospholipid 0.1
(11) Carboxyvinyl polymer aqueous solution (1.0%) 10.0
(12) Sodium hydroxide 0.05
(13) Glycerin 5.0
(14) 1,3-butylene glycol 7.0
(15) Purified water remaining amount (16) sengoku flower extract: * 1 0.1
(17) Ethyl alcohol 5.0
(18) Silicic anhydride 1.0
(19) Preservative appropriate amount (20) Fragrance appropriate amount * 1 Production Example 5

(Manufacturing method)
A. Ingredients (1) to (10) are heated and mixed and maintained at 70 ° C.
B. Ingredients (11) to (15) are heated and mixed and maintained at 70 ° C.
C. Add B to A, mix and uniformly emulsify.
D. After cooling C, (16) and (17) to (20) were added and mixed uniformly to obtain an emulsion.

  Example 4 is stable without discoloration, odor, separation, etc., and has a smooth moisturizing feeling when applied to the skin, and continuously applied to suppress skin disorders such as wrinkle formation, skin thickening, and skin hardening. Or it had the effect of improving.

Example 5 Pack (ingredient) (%)
(1) Polyvinyl alcohol 15.0
(2) Silicic anhydride 0.5
(3) Polyethylene glycol 0.5
(4) Polyoxypropylene methyl glucoside 5.0
(5) Glycerin 5.0
(6) Purified water remaining amount (7) Ethyl alcohol 10.0
(8) Preservative Appropriate amount (9) French chrysanthemum flower extract * 1 0.05
(10) Perfume appropriate amount * 1 Production Example 6

(Manufacturing method)
A. Components (1) to (6) are mixed and heated to 70 ° C. to dissolve.
B. Components (7) to (8) are mixed and dissolved.
C. B was added to the previous A, mixed, and then cooled to uniformly disperse (9) and (10) to obtain a pack.

  Example 5 is stable without discoloration, odor, separation, etc. When applied to the skin, it has a moderate tension, and after peeling off the pack, the skin has a high moist feeling, wrinkle formation, skin thickening, skin It was excellent in the effect of suppressing or reducing skin disorders such as curing.

Example 6 Liquid foundation (component) (%)
(1) Dipentaerythritol tetra 12-hydroxystearic acid sesquistearic acid hemirosin ester 2.0
(2) Liquid paraffin 5.0
(3) Stearic acid 2.0
(4) Cetanol 1.0
(5) Self-emulsifying glyceryl monostearate 1.0
(6) Paramethoxycinnamic acid-2-ethylhexyl 8.0
(7) Feverfew whole plant extract * 1 0.01
(8) Preservative appropriate amount (9) Glycerin 5.0
(10) Triethanolamine 1.0
(11) Carboxymethylcellulose 0.2
(12) Bentonite 0.5
(13) Purified water remaining amount (14) Titanium oxide 6.0
(15) Fine particle titanium oxide 2.0
(16) Fine zinc oxide 5.0
(17) Mica 2.0
(18) Talc 4.0
(19) Color pigment 4.0
(20) Perfume appropriate amount * 1 Production Example 9

(Manufacturing method)
A. Components (1) to (6) are heated and mixed and dissolved.
B. Ingredients (14) to (19) are added to A, mixed uniformly, and kept at 70 ° C.
C. Ingredients (8) to (13) are uniformly dissolved and kept at 70 ° C.
D. B is added to C and emulsified uniformly.
E. After cooling D, components (7) and (20) were added to obtain a liquid foundation.

  Example 6 is stable with no odor, separation, etc., and when applied to the skin, is excellent in a moisturizing makeup effect, moderately protects the skin from ultraviolet rays during the day, wrinkles, skin thickening, skin hardening, etc. It was excellent in the effect of suppressing or reducing skin damage.

Example 7 Sunscreen Latex (Component) (%)
(1) Polyoxyalkylene-modified organopolysiloxane 1.0
(2) Dimethylpolysiloxane 5.0
(3) Octamethylcyclotetrasiloxane 20.0
(4) Isotridecyl isononanoate 5.0
(5) Paramethoxycinnamic acid-2-ethylhexyl 5.0
(6) Fine particle titanium oxide 10.0
(7) Fine zinc oxide 10.0
(8) Zirconium oxide 5.0
(9) Polystyrene powder 3.0
(10) Trimethylsiloxysilicate 0.5
(11) Preservative appropriate amount (12) Dipropylene glycol 3.0
(13) Ethyl alcohol 10.0
(14) Purified water remaining amount (15) Salt 0.2
(16) Chrysanthemum leaf extract * 1 0.2
(17) Perfume appropriate amount * 1 Production Example 8

(Manufacturing method)
A. Components (1) to (10) are mixed and dispersed.
B. Components (11) to (15) are mixed and dissolved.
C. Add B to A and emulsify uniformly.
D. Components (16) and (17) were added to C to obtain a sunscreen emulsion.

  Example 7 is stable with no odor, separation, etc., and when applied to the skin, has a refreshing emollient effect, protects the skin from ultraviolet rays during the day, and prevents skin disorders such as wrinkle formation, skin thickening, and skin hardening. It was excellent in the effect of suppressing or reducing.

Claims (1)

Ryuunougiku (Chrysanthemum Makino Matsum.etNakai), Kikutanigiku (Chrysanthemum boreale Makino), Shimakangiku (Chrysanthemum indicum L.), chrysanthemum pacificum (Chrysanthemum pacificum), garland chrysanthemum (Chrysanthemum coronarium), and selected from the group consisting of French Chrysanthemum (Chrysanthemum Leucanthemum L.) A skin external preparation for anti-aging which exhibits an action to suppress abnormal accumulation of skin extracellular matrix components caused by exposure to ultraviolet rays, or an action to suppress skin thickening caused by exposure to ultraviolet rays, comprising at least one selected from the above as an active ingredient.