JP4881046B2 - Cryopreservation - Google Patents
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Description
本発明は、採取した各種試料（例えば、海底の堆積物や岩盤から採取した微生物含有のコアサンプルや臓器等）を採取場所（例えば、船舶等）から所定の目的場所（例えば、陸上の研究施設等）へと凍結保存状態で運搬することができる凍結保存器に関するものである。 In the present invention, various samples collected (for example, core samples and organs containing microorganisms collected from seabed sediments and bedrock) from a collection location (for example, a ship) to a predetermined destination location (for example, a research facility on land) To a cryopreservation device that can be transported in a cryopreserved state.
例えば、日米主導の国際的な科学プロジェクトである統合国際深海掘削計画（ＩＯＤＰ）にあっては、海底の堆積物や岩盤からコアサンプルを採取して、そのサンプルに含まれる微生物等についての生物研究を行なうことが計画されているが、このような生物研究を行なう場合、船上では実験設備等が十分でないため、採取したコアサンプルの一部（試料）を船上で凍結保存して、実験設備等の充実する陸上の研究施設へと持ち帰る必要がある。 For example, in the Integrated International Deep Drilling Program (IODP), an international scientific project led by Japan and the United States, core samples are collected from sediments and bedrock on the seabed, and organisms such as microorganisms contained in the samples are collected. It is planned to conduct research. However, when such biological research is conducted, because there are not enough experimental facilities on board, some of the collected core samples (samples) are frozen and stored on the ship. It is necessary to take it back to a well-equipped onshore research facility.
このような試料の海上から陸上への運搬は、一般に、試料と共にドライアイスを収納した断熱容器を使用して行なわれている。しかし、このようなドライアイスを使用した凍結保存では、凍結保存状態が十分でないため、航海が長期に亘って行われる場合、陸上の研究施設に運搬されるまでの間に試料に含まれる微生物が死滅してしまう虞れがあった。 In general, such a sample is transported from the sea to the land using a heat insulating container containing dry ice together with the sample. However, in such cryopreservation using dry ice, the cryopreservation state is not sufficient, so if the voyage is carried out over a long period of time, the microorganisms contained in the sample will not be contained before being transported to a land research facility. There was a risk of dying.
このため、例えば非特許文献１に示されるように、極低温液化ガスである液体窒素を貯留した断熱容器を使用することも試みられている。このような液体窒素を使用した凍結保存方法によれば、試料の凍結保存が十分に行われ、航海が長期に亘る場合にも、試料に含まれる微生物の死滅を可及的に防止することができる。 For this reason, as shown in Non-Patent Document 1, for example, it has been attempted to use a heat insulating container storing liquid nitrogen that is a cryogenic liquefied gas. According to such a cryopreservation method using liquid nitrogen, it is possible to prevent the microorganisms contained in the sample from being killed as much as possible even when the sample is sufficiently frozen and preserved for a long period of time. it can.
しかし、このような液体窒素を貯留した断熱容器を使用する場合には、試料の断熱容器への収納作業や断熱容器の運搬作業等において、液体窒素が漏洩する危険があり、不安定な船上での作業を安全に行い難いといった問題があった。 However, when using an insulated container that stores such liquid nitrogen, there is a risk of liquid nitrogen leaking during storage of the sample into the insulated container or transportation of the insulated container. There was a problem that it was difficult to perform this work safely.
本発明は、このような問題を生じることなく、船上等の採取場所において試料を十分に凍結保存し、試料を陸上研究施設等の目的場所へと良好な凍結保存状態で安全に運搬することができる凍結保存器を提供することを目的とする。 In the present invention, without causing such a problem, the sample can be sufficiently frozen and stored at a sampling place on the ship, and the sample can be safely transported to a target place such as a land research facility in a good frozen storage state. An object of the present invention is to provide a cryopreservation device that can be used.
本発明は、上記の目的を達成すべく、上部に開閉自在な試料出入口を有する横断面形状円形の断熱容器と、試料出入口から断熱容器内に格納される試料収納ケースと、断熱容器内に配置された極低温液化ガスの含浸材とを具備して、含浸材に含浸された液化ガスにより断熱容器内を極低温の気相雰囲気に保持するように構成したことを特徴とする凍結保存器を提案する。
In order to achieve the above object, the present invention provides a circular insulated cross-sectional heat insulating container having a sample opening / closing port that can be opened and closed at the top, a sample storage case stored in the heat insulating container from the sample inlet / outlet, and disposed in the heat insulating container. A cryopreservation device comprising: a cryogenic liquefied gas impregnated material, and the liquefied gas impregnated in the impregnated material configured to hold the inside of the heat insulating container in a cryogenic gas phase atmosphere. suggest.
かかる凍結保存器にあっては、断熱容器が複数の試料収納ケースを当該断熱容器の周方向に等間隔を隔てて格納しうるものであり、断熱容器内には、各試料収納ケースの格納領域及びこれらの格納領域から試料出入口へと至る試料収納ケースの出し入れ領域を除いて、極低温液化ガスの含浸材が充填されている。また、含浸材はグラスファイバペーパを積層してなるものであって、断熱容器の底部領域である出し入れ領域及び各格納領域の下部領域に充填された第１含浸材と格納領域相互間の各領域に充填された横断面形状が扇形をなす柱状の第２含浸材とからなる。また、各試料収納ケースは、横断面形状が円形をなす複数の試料収容箱と、これらの試料収容箱を上下に積層した状態で保持する保持枠と、試料収容箱を保持した保持枠を収納する有底円筒状の収納体及びこれに取り付けられて上方に延びる吊支杆からなるキャニスタとを具備して、吊支杆の上端部に形成した係合部を試料出入口の周縁部に係合させることにより、断熱容器内の格納領域に吊支状態で格納しうるように構成されている。各試料収納ケースは吊支杆を除いて金属製のものであり、吊支杆が、金属製の係合部とキャニスタの収納体に取付けられる金属製の取付部とをプラスチック製の中間部で連結してなるものである。また、各試料収容箱は、海底より採取される柱状コアサンプルから得られた大型の円形試料を収納しうる大きさのものであることが好ましい。
In such a cryopreservation device, the heat insulation container is capable of storing a plurality of sample storage cases at equal intervals in the circumferential direction of the heat insulation container, and in the heat insulation container, the storage area of each sample storage case and except for the loading and unloading area of the sample storage case leading to the sample inlet and outlet from these storage areas, impregnant cryogenic liquefied gas that has been filled. Further, the impregnating material is formed by laminating glass fiber paper, and each region between the first impregnating material and the storage region filled in the loading / unloading region which is the bottom region of the heat insulating container and the lower region of each storage region. And a columnar second impregnating material in which the cross-sectional shape filled in is a fan shape. Each sample storage case stores a plurality of sample storage boxes having a circular cross-sectional shape, a holding frame that holds these sample storage boxes in a stacked state, and a holding frame that holds the sample storage box. And a canister composed of a suspended support attached to the bottom and extending upwardly, and an engaging portion formed on the upper end of the suspended support is engaged with the peripheral edge of the sample inlet / outlet By being made, it is comprised so that it can store in the storage area in a heat insulation container in a suspended state . Each sample storage case is made of metal except for the suspension support, and the suspension support is made up of a metal engagement portion and a metal attachment portion attached to the canister storage body with a plastic intermediate portion. Ru der those formed by connecting. Moreover, it is preferable that each sample storage box is a thing of the magnitude | size which can accommodate the large-sized circular sample obtained from the columnar core sample extract | collected from the seabed.
本発明の凍結保存器は、液体窒素等の極低温液化ガスをグラスファイバペーパ等の含浸材に含浸，保持させて、断熱容器内を極低温の気相雰囲気に保持しておくものであるから、断熱容器内に収納した試料を、ドライアイスを使用する場合に比して、十分に凍結保存することができると共に、液体窒素を貯留させておく場合に比して、試料の凍結保存作業や採取場所から海路，空路，陸路を経て目的場所への試料の運搬作業を安全に行うことができ、特に、海上で採取した試料の生物研究等を行なう上で極めて便利なものである。また、試料収納ケースを横断面形状が円形をなす複数の試料収容箱を上下に積層した状態で保持するものとしておくことにより、各試料収容箱に冒頭で述べたコアサンプルや臓器等の大型試料を細分化することなく、そのまま収納しておくことができ、かかる大型試料の凍結保存を簡便に行いうる。また、キャニスタ吊支杆の一部（中間部）を伝熱係数の低いエポキシ樹脂等のプラスチックで構成しておくことにより、試料収納ケースを吊支杆を介して断熱容器の試料出入口に吊支させておく場合にも、吊支杆からの熱損失を可及的に防止し得て、試料の凍結保存を良好に行うことができる。 Since the cryopreservation device of the present invention impregnates and holds a cryogenic liquefied gas such as liquid nitrogen in an impregnating material such as glass fiber paper, the inside of the heat insulating container is kept in a cryogenic gas phase atmosphere. In addition, the sample stored in the heat insulating container can be stored frozen as compared with the case of using dry ice, and the sample can be cryopreserved as compared with the case of storing liquid nitrogen. The sample can be safely transported from the collection location to the destination location via the sea, air, and land routes, and is particularly convenient for conducting biological research on samples collected at sea. In addition, by holding the sample storage cases in a state where a plurality of sample storage boxes having a circular cross-sectional shape are stacked one above the other, large samples such as core samples and organs described at the beginning of each sample storage box Can be stored as it is without being subdivided, and the large sample can be cryopreserved easily. In addition, by constructing a part (intermediate part) of the canister suspension support with a plastic such as epoxy resin having a low heat transfer coefficient, the sample storage case is suspended from the sample inlet / outlet of the heat insulating container via the suspension support. Also when it is allowed to keep, heat loss from the suspension support can be prevented as much as possible, and the sample can be cryopreserved satisfactorily.
また、収納箱及び保持枠を用いることにより、コアサンプル等を他の収納箱に移し替える事無く、保管場所にある大型の凍結保存容器に保管することが可能となる。これにより、試料の移し替えに伴う、試料の昇温を防ぐことが可能となり、より安全に試料を輸送・保管することができる。 Further, by using the storage box and the holding frame, the core sample or the like can be stored in a large cryopreservation container in a storage place without being transferred to another storage box. As a result, it is possible to prevent the temperature of the sample from increasing due to the transfer of the sample, and the sample can be transported and stored more safely.
また、従来、輸送したサンプルを保存する場合、輸送用の小型凍結保存容器のキャニスタから保存先の大型凍結保存容器のラック（収納箱及び保持枠）に移し替える必要があり、移し替えの際にサンプルの温度が昇温し、サンプルが死滅する可能性があった。しかし、本発明の凍結保存器によれば、試料収納ケース（収納箱、保持枠及び収納体）を用いることにより、サンプルの保管先で収納箱及び保持枠を取り出し、そのまま大型の凍結保存容器に移し替えることが可能となる為、サンプルの昇温を可能な限り減らすことができる。このように、本発明の凍結保存器は大型サンプルの運搬，保存システムとして好適するものであり、その実用的価値極めて大なるものである。 Also, conventionally, when transported samples are stored, they must be transferred from the canister of the small cryopreservation container for transportation to the rack (storage box and holding frame) of the large cryopreservation container at the storage destination. There was a possibility that the temperature of the sample increased and the sample was killed. However, according to the cryopreservation device of the present invention, by using the sample storage case (storage box, holding frame and storage body), the storage box and the holding frame are taken out at the storage location of the sample and can be directly used as a large cryopreservation container. Since it can be transferred, the temperature of the sample can be reduced as much as possible. Thus, the cryopreservation device of the present invention is suitable as a large sample transport and storage system, and its practical value is extremely large.
図１は本発明の凍結保存器１を示す縦断正面図であり、図２はその横断平面図であり、図３〜図５はその要部を示す斜視図である。 FIG. 1 is a longitudinal front view showing a cryopreservation device 1 of the present invention, FIG. 2 is a cross-sectional plan view thereof, and FIGS. 3 to 5 are perspective views showing an essential part thereof.
凍結保存器１は、図１及び図２に示す如く、断熱容器２内に複数の試料収納ケース３を格納すると共に極低温液化ガスの含浸材４を充填してなる。 As shown in FIGS. 1 and 2, the cryopreservation unit 1 stores a plurality of sample storage cases 3 in a heat insulating container 2 and is filled with an impregnating material 4 of a cryogenic liquefied gas.
断熱容器２は、図１及び図２に示す如く、上部にキャップ２１により開閉される試料出入口２２を有する横断面形状円形のものである。試料出入口２２は、後述する横断面円形の試料収納ケース３を出し入れするに必要且つ十分な径（この例では、１３０ｍｍ）を有する円形状をなしている。断熱容器２の容積は、格納しようとする試料収納ケース３の数に応じて設定されるが、この例では、３個の試料収納ケース３を格納しうるに必要且つ十分な容積（５５Ｌ）に設定されている。断熱容器２の周壁２３は、アルミニウム製の二重壁構造であって、断熱材２４を充填した真空構造をなしている。なお、周壁２４内には、図示していないが、断熱材２４と共に適宜の吸着材が装填されている。 As shown in FIGS. 1 and 2, the heat insulating container 2 has a circular cross-sectional shape having a sample inlet / outlet 22 opened and closed by a cap 21 at the top. The sample inlet / outlet port 22 has a circular shape having a diameter (130 mm in this example) that is necessary and sufficient for taking in and out a sample storage case 3 having a circular cross section described later. The volume of the heat insulating container 2 is set according to the number of sample storage cases 3 to be stored. In this example, the volume is sufficient and sufficient to store the three sample storage cases 3 (55 L). Is set. The peripheral wall 23 of the heat insulating container 2 has a double wall structure made of aluminum and has a vacuum structure filled with a heat insulating material 24. Although not shown, an appropriate adsorbent is loaded in the peripheral wall 24 together with the heat insulating material 24.
各試料収納ケース３は、図３に示す如く、複数の試料収容箱３１と保持枠３２とキャニスタ３３とからなる。 Each sample storage case 3 includes a plurality of sample storage boxes 31, a holding frame 32, and a canister 33 as shown in FIG.
各試料収容箱３１は、図５に示す如く、上面を開放した横断面形状が円形をなすもので、大型の円形試料５をそのまま収容できる大きさのものである。この例では、冒頭で述べた如く海底の堆積物や岩盤から採取される柱状コアサンプルを輪切りした円形試料５を収納しうるに十分な径及び深さを有するものとされている。具体的には、コアサンプルの採取は船上から内径１００ｍｍのドリルを使用して掘削すること等により行なわれるものであるから、試料収容箱３１の径及び深さは夫々１０５ｍｍ及び５０ｍｍとされている。なお、試料収容箱３１の周壁には、これを後述する保持枠３２に出し入れするための操作孔３１ａが形成されている。また、試料収容箱３１の底壁３１ｂは、パンチングメタル等の透孔板で構成されている。 As shown in FIG. 5, each sample storage box 31 has a circular cross-sectional shape with an open upper surface, and has a size that can store a large circular sample 5 as it is. In this example, as described at the beginning, the diameter and the depth are sufficient to accommodate the circular specimen 5 obtained by cutting the columnar core sample collected from the seabed sediment or rock. Specifically, since the core sample is collected by drilling from the ship using a drill with an inner diameter of 100 mm, the diameter and depth of the sample storage box 31 are 105 mm and 50 mm, respectively. . An operation hole 31a is formed on the peripheral wall of the sample storage box 31 for taking it in and out of a holding frame 32 described later. Further, the bottom wall 31b of the sample storage box 31 is formed of a through-hole plate such as a punching metal.
保持枠３２は、図５に示す如く、半円筒状の本体部３２ａと、本体部３２ａの上下端部に固着された上下壁部３２ｂ，３２ｃと、上下壁部３２ｂ，３２ｃ間に等間隔を隔てて配置した状態で本体部３２ａに固着された複数の仕切壁部３２ｄと、上壁部３２ｂに取付けられたフック３２ｅとからなり、上下壁部３２ｂ，３２ｃ及び仕切壁部３２ｄの相互間に各々試料収容箱３１を挿脱させることができ、複数の試料収容箱３１を上下に積層した状態で保持しうるようになっている。なお、上下壁部３２ｂ，３２ｃ及び各仕切壁部３２ｄは、試料収容箱３１と同一径の円板形状をなすものであり、これの外周部の半分が本体部３２ａに溶接等により固着されている。 As shown in FIG. 5, the holding frame 32 has a semi-cylindrical main body portion 32a, upper and lower wall portions 32b and 32c fixed to the upper and lower ends of the main body portion 32a, and equal intervals between the upper and lower wall portions 32b and 32c. It consists of a plurality of partition wall portions 32d fixed to the main body portion 32a in a state of being spaced apart, and a hook 32e attached to the upper wall portion 32b, and between the upper and lower wall portions 32b, 32c and the partition wall portion 32d. Each of the sample storage boxes 31 can be inserted and removed, and a plurality of sample storage boxes 31 can be held in a stacked state. The upper and lower wall portions 32b and 32c and each partition wall portion 32d are formed in a disk shape having the same diameter as the sample storage box 31, and half of the outer peripheral portion thereof is fixed to the main body portion 32a by welding or the like. Yes.
キャニスタ３３は、図３及び図４に示す如く、試料収容箱３１を保持した保持枠３２を収納しうる有底円筒状の収納体３３ａと、これに溶接等により取り付けられて上方に延びる吊支杆３３ｂとからなる。収納体３３ａの内径及び深さは、保持枠３２を収納しうるに必要且つ最小限の寸法に設定されている。吊支杆３３ｂは、上端部を試料出入口２２の周縁部に係合しうる屈曲状の係合部３３ｃと、収納体３３ａに取り付けられた取付部３３ｄと、両部３３ｃ，３３ｃ間を連結する中間部３３ｅとからなり、係合部３３ｃを試料出入口２２の周縁部に係合させることにより、キャニスタ３３を、断熱容器２内に吊支させておくことができるようになっている。この例では、試料出入口２２の内周部に、図示していないが、周方向に等間隔（１２０度）を隔てて上下方向に延びる３本の係合溝が形成されており、各吊支杆３３ｂの中間部３３ｅをこの係合溝に係合させることにより、３個の試料収納ケース３つまりキャニスタ３３を、断熱容器２内の予め設定された領域に吊支保持させておくことができるようになっている。すなわち、かかる吊支保持状態においては、３個のキャニスタ３３の収納体３３ａが、図２に示す如く、断熱容器２内における試料出入口２２の直下領域２ａを除く円筒状領域に、周方向に等間隔（１２０度）を隔てて位置されるのである。以下、各収納体３３ａが位置する断熱容器２内の領域を試料収納ケースの格納領域２ｂという。そして、各試料収納ケース３つまりキャニスタ３３は、吊支杆３３ｂを操作して、収納体３３ａを格納領域２ｂから試料出入口２２の直下領域２ａにもたらすことによって、試料出入口２２から出し入れすることができる。以下、この試料収納ケース３を出し入れするための上記直下領域を試料収納ケースの出し入れ領域２ａという。 3 and 4, the canister 33 has a bottomed cylindrical storage body 33a that can store a holding frame 32 that holds the sample storage box 31, and a suspension support that is attached to this by welding or the like and extends upward. It consists of a bag 33b. The inner diameter and depth of the storage body 33a are set to the minimum and necessary dimensions for storing the holding frame 32. The suspension support rod 33b connects between the bent portions 33c and 33c, a bent engagement portion 33c whose upper end portion can be engaged with the peripheral edge portion of the sample inlet / outlet port 22, a mounting portion 33d attached to the storage body 33a. The canister 33 can be suspended in the heat insulating container 2 by engaging the engaging portion 33c with the peripheral portion of the sample inlet / outlet port 22. In this example, although not shown in the drawing, three engagement grooves extending in the vertical direction at equal intervals (120 degrees) in the circumferential direction are formed in the inner peripheral portion of the sample inlet / outlet port 22. By engaging the intermediate portion 33e of the flange 33b with the engagement groove, the three sample storage cases 3, that is, the canisters 33 can be suspended and held in a preset region in the heat insulating container 2. It is like that. That is, in such a suspended support holding state, the storage body 33a of the three canisters 33 is provided in a cylindrical region excluding the region 2a directly below the sample inlet / outlet 22 in the heat insulating container 2 as shown in FIG. They are positioned at an interval (120 degrees). Hereinafter, the area in the heat insulating container 2 where the storage bodies 33a are located is referred to as a storage area 2b of the sample storage case. Each sample storage case 3, that is, the canister 33, can be taken in and out of the sample inlet / outlet 22 by operating the suspension support rod 33 b to bring the storage body 33 a from the storage area 2 b to the area 2 a immediately below the sample inlet / outlet 22. . Hereinafter, the region directly under which the sample storage case 3 is taken in and out is referred to as a sample storage case in / out region 2a.
ところで、試料収納ケース３の各構成部材は、吊支杆３３ｂの中間部３３ｅを除いて、耐低温性に優れた金属（例えば、ＳＵＳ３０４等のステンレス鋼）で構成されている。一方、吊支杆３３ｂの中間部３３ｅは、吊支杆３３ｂからの熱伝導による熱損失を防止するために、伝熱係数の低い高強度プラスチック（この例では、エボキシ樹脂）で構成されている。 By the way, each component of the sample storage case 3 is made of a metal excellent in low temperature resistance (for example, stainless steel such as SUS304) except for the intermediate portion 33e of the hanging support rod 33b. On the other hand, the intermediate portion 33e of the suspension support rod 33b is made of high-strength plastic (in this example, epoxy resin) having a low heat transfer coefficient in order to prevent heat loss due to heat conduction from the suspension support rod 33b. .
含浸材４は、図１及び図２に示す如く、断熱容器２内における出し入れ領域２ａ及び各格納領域２ｂを除く領域２ｃ，２ｄに充填されている。含浸材４には極低温液化ガス（この例では液体窒素）が含浸，保持されていて、出し入れ領域２ａ及び各格納領域２ｂを含む断熱容器２の内部領域を極低温の気相雰囲気に保持する。すなわち、含浸材４は、断熱容器２の底部領域（出し入れ領域２ａ及び各格納領域２ｂの下部領域）２ｃに充填された円板状の第１含浸材４１と、３つの格納領域２ｂ相互間の各領域２ｄに充填された横断面形状が扇状をなす柱状の第２含浸材４２とからなる。各含浸材４１，４２は、液体窒素を含浸（吸収），保持できるものであればよいが、この例では、グラスファイバペーパを積層したものが使用されている。含浸材４への液体窒素の含浸は、例えば、断熱容器２内に液体窒素を充填した上、余剰の液体窒素をサイホン管等を使用して、断熱容器２から排出させることによって行なわれる。ところで、液体窒素（沸点：−１９６℃）の含浸材４を使用することにより、断熱容器２内は−１５０℃以下の極低温雰囲気に保持される。−１５０℃以下である極低温雰囲気の保持時間は含浸材４の充填容積等によって異なるが、この例のものでは、試料５を保存した状態で３０日程度となる。 As shown in FIGS. 1 and 2, the impregnating material 4 is filled in regions 2c and 2d excluding the take-in / out region 2a and the storage regions 2b in the heat insulating container 2. The impregnating material 4 is impregnated and held with a cryogenic liquefied gas (in this example, liquid nitrogen), and the inner region of the heat insulating container 2 including the take-in / out region 2a and each storage region 2b is held in a cryogenic gas phase atmosphere. . That is, the impregnating material 4 is formed between the disc-shaped first impregnating material 41 filled in the bottom region (the lower region of the storage region 2a and the storage region 2b) 2c of the heat insulating container 2 and the three storage regions 2b. Each of the regions 2d is composed of a columnar second impregnating material 42 in which the cross-sectional shape is a fan shape. Each impregnating material 41 and 42 may be any material that can impregnate (absorb) and hold liquid nitrogen, but in this example, a laminated glass fiber paper is used. The impregnation material 4 is impregnated with liquid nitrogen, for example, by filling the heat insulating container 2 with liquid nitrogen and discharging excess liquid nitrogen from the heat insulating container 2 using a siphon tube or the like. By the way, by using the impregnating material 4 of liquid nitrogen (boiling point: −196 ° C.), the inside of the heat insulating container 2 is kept in an extremely low temperature atmosphere of −150 ° C. or less. Although the holding time of the cryogenic atmosphere at −150 ° C. or less varies depending on the filling volume of the impregnating material 4 and the like, in this example, it is about 30 days with the sample 5 stored.
したがって、以上のように構成された凍結保存器１によれば、海上において、コアサンプルから得た円形試料５を試料収容箱３１に収容し、試料収容箱３１を保持枠３２に保持させた上でキャニスタ３により断熱容器２内に格納することにより、試料５を瞬時に且つ十分に凍結して、試料５に含まれる微生物を良好に凍結保存することができる。このため、ドライアイスを使用した場合のように、陸上の研究施設への運搬途中に微生物が死滅するようなことがない。また、液体窒素が含浸材４に含浸，保持されていることから、不安定な船舶上における試料５の凍結保存作業（試料収納ケース３の断熱容器２内への格納作業）や運搬作業において液体窒素が断熱容器２から漏洩するといった危険がなく、これらの作業を極めて安全に行なうことができる。 Therefore, according to the cryopreservation device 1 configured as described above, the circular sample 5 obtained from the core sample is stored in the sample storage box 31 and the sample storage box 31 is held in the holding frame 32 at sea. By storing in the heat insulating container 2 by the canister 3, the sample 5 can be frozen instantly and sufficiently, and the microorganisms contained in the sample 5 can be well frozen and stored. For this reason, unlike when dry ice is used, microorganisms do not die during transportation to a land-based research facility. Further, since the liquid nitrogen is impregnated and held in the impregnating material 4, the sample 5 is frozen and stored on an unstable ship (storage work in the heat insulating container 2 of the sample storage case 3) or transported. There is no danger of leakage of nitrogen from the heat insulating container 2, and these operations can be performed extremely safely.
なお、本発明は上記した実施の形態に限定されるものではなく、本発明の基本原理を逸脱しない範囲において適宜に改良，変更することができる。例えば、断熱容器２内に格納する試料収納ケース３の数は、断熱容器２の容積等に応じて任意に設定することができ、１個の試料収納ケース３を格納するように構成することもできる。この場合、上記出し入れ領域２ａを試料収納ケースの格納領域とし、上記格納領域２ｂを全て含浸材４の充填領域として使用することができ、凍結保存時間（−１５０℃以下に保持できる時間）を増大させることができ、試料の採取から目的場所への運搬までに要する時間が長い場合（例えば、航海期間が長い場合）にも、試料５の凍結保存を確実に行うことができる。また、本発明の凍結保存器１は、前記したコアサンプルや臓器等の大型試料を凍結保存する場合に最適するものであるが、アンプル等に収納される小型試料や微細試料を凍結保存する場合にも好適に使用することができる。 It should be noted that the present invention is not limited to the above-described embodiment, and can be appropriately improved and changed without departing from the basic principle of the present invention. For example, the number of sample storage cases 3 stored in the heat insulating container 2 can be arbitrarily set in accordance with the volume of the heat insulating container 2 and the like, and a single sample storage case 3 can be stored. it can. In this case, the loading / unloading area 2a can be used as a storage area for the sample storage case, and the storage area 2b can be used as a filling area for the impregnating material 4. Even when the time required from collection of the sample to transportation to the destination is long (for example, when the voyage period is long), the sample 5 can be reliably frozen and stored. The cryopreservation device 1 of the present invention is optimal for cryopreserving large samples such as the core samples and organs described above, but in the case of cryopreserving small samples and fine samples stored in ampoules and the like. Also, it can be suitably used.
１ 凍結保存器
２ 断熱容器
２ａ 出し入れ領域
２ｂ 格納領域
３ 試料収納ケース
４ 含浸材
５ 試料
２２ 試料出入口
３１ 試料収容箱
３２ 保持枠
３３ キャニスタ
３３ａ 収納体
３３ｂ 吊支杆
３３ｃ 吊支杆の係合部
３３ｄ 吊支杆の取付部
３３ｅ 吊支杆の中間部
４１ 第１含浸材
４２ 第２含浸材
DESCRIPTION OF SYMBOLS 1 Cryopreservation device 2 Insulation container 2a Out / in area 2b Storage area 3 Sample storage case 4 Impregnation material 5 Sample 22 Sample inlet / outlet 31 Sample storage box 32 Holding frame 33 Canister 33a Storage body 33b Suspension support 33c Engagement section 33d of suspension support Attachment part of hanging support 33e Middle part of hanging support
41 1st impregnating material
42 second impregnating material
Claims (2)
断熱容器（２）が複数の試料収納ケース（３）を当該断熱容器（２）の周方向に等間隔を隔てて収納しうるものであり、断熱容器（２）内には、各試料収納ケース（３）の格納領域（２ｂ）及びこれらの格納領域（２ｂ）から試料出入口（２２）へと至る試料収納ケース（３）の出し入れ領域（２ａ）を除いて、極低温液化ガスの含浸材（４）が充填されており、
含浸材（４）はグラスファイバペーパを積層してなるものであって、断熱容器（２）の底部領域である出し入れ領域（２ａ）及び各格納領域（２ｂ）の下部領域（２ｃ）に充填された第１含浸材（４１）と格納領域（２ｂ）相互間の各領域（２ｄ）に充填された横断面形状が扇形をなす柱状の第２含浸材（４２）とからなり、
各試料収納ケース（３）が、横断面形状が円形をなす複数の試料収容箱（３１）と、これらの試料収容箱（３１）を上下に積層した状態で保持する保持枠（３２）と、試料収容箱（３１）を保持した保持枠（３２）を収納する有底円筒状の収納体（３３ａ）及びこれに取り付けられて上方に延びる吊支杆（３３ｂ）からなるキャニスタ（３３）とを具備して、吊支杆（３３ｂ）の上端部に形成した係合部（３３ｃ）を試料出入口（２２）の周縁部に係合させることにより、断熱容器（２）内の格納領域（２ｂ）に吊支状態で格納されるように構成されたものであり、
各試料収納ケース（３）が吊支杆（３３ｂ）を除いて金属製のものであり、各試料収納ケース（３）の吊支杆（３３ｂ）が金属製の係合部（３３ｃ）とキャニスタ（３３）の収納体（３３ａ）に取り付けられる金属製の取付部（３３ｄ）とをプラスチック製の中間部（３３ｅ）で連結してなるものであることを特徴とする凍結保存器。 A heat insulating container (2) having a circular cross-sectional shape having a sample inlet / outlet (22) that can be opened and closed at the top, a sample storage case (3) stored in the heat insulating container (2) from the sample inlet / outlet (22 ), and a heat insulating container (2) and a impregnant loading cryogenic liquefied gas (4) in, by impregnating the impregnated material (4) liquefied gas insulated container (2) in the gas phase atmosphere cryogenic A cryopreservation device configured to hold ,
The heat insulating container (2) can store a plurality of sample storage cases (3) at equal intervals in the circumferential direction of the heat insulating container (2), and each sample storage case is contained in the heat insulating container (2). Excluding the storage area (2b) of (3) and the storage area (2a) of the sample storage case (3) extending from the storage area (2b) to the sample inlet / outlet (22), the impregnating material for cryogenic liquefied gas ( 4) is filled,
The impregnating material (4) is formed by laminating glass fiber papers, and is filled in a loading / unloading region (2a) which is a bottom region of the heat insulating container (2) and a lower region (2c) of each storage region (2b). The first impregnating material (41) and the storage region (2b) are each composed of a columnar second impregnating material (42) in which each region (2d) filled between the storage regions (2b) forms a sector shape,
Each sample storage case (3) has a plurality of sample storage boxes (31) having a circular cross-sectional shape, and a holding frame (32) for holding these sample storage boxes (31) in a stacked state. A bottomed cylindrical storage body (33a) for storing a holding frame (32) holding a sample storage box (31), and a canister (33) which is attached to this and has a hanging support rod (33b) extending upward. And a storage region (2b) in the heat insulating container (2) by engaging an engaging portion (33c) formed at the upper end of the suspension support rod (33b) with the peripheral portion of the sample inlet / outlet (22). It is configured to be stored in a suspended state in
Each sample storage case (3) is made of metal except for the suspension support rod (33b), and the suspension support rod (33b) of each sample storage case (3) is made of a metal engaging portion (33c) and a canister. A cryopreservation device comprising a metal attachment portion (33d) attached to the storage body (33a) of (33) and a plastic intermediate portion (33e) .
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| JPS6288938A (en) * | 1985-10-15 | 1987-04-23 | Shimizu Constr Co Ltd | Equipment for transporting and storing specimens in frozen sampling of gravel ground |
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| JP2005143873A (en) * | 2003-11-17 | 2005-06-09 | Taiyo Nippon Sanso Corp | Cryopreservation container |
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