JP4937583B2 - Oral composition - Google Patents

Description

  The present invention relates to a remineralization accelerator for tooth enamel and an oral composition produced by adding the remineralization accelerator.

  Caries start as caries bacteria adhere to the tooth surface and form plaque, and the acid produced by the caries fungus metabolizing food in the plaque demineralizes the tooth enamel. Create a carious state. Saliva has the function of remineralizing the demineralized part by the action of calcium and phosphorus in the saliva and returning the teeth to the original state.

In order to promote remineralization, a dentifrice containing one kind of fluoride and calcium phosphate and hydroxyapatite having a crystal structure similar to that of an inorganic tooth component has been manufactured. However, the remineralization of the demineralized part is not sufficient only by using saliva, fluoride, or hydroxyapatite dentifrice.
Apart from this, a composition for oral cavity containing silk powder is known.

  As a dentifrice using silk powder, a composition for oral cavity containing silk powder and dextranase (Japanese Patent Laid-Open No. 2000-63251) and an enzyme were immobilized in order to enhance the effect of suppressing the reattachment of plaque. By providing a disintegrating silk powder, a composition for oral cavity which is excellent in adhesion prevention effect and removal action of plaque and excellent in feeling of use is provided (Japanese Patent Laid-Open No. 2001-226243), sucrose fatty acid ester and collagen compound A dentifrice composition (Japanese Patent Laid-Open No. 2001-278757) formulated with silk powder to enhance UV absorption effect, and for oral cavity containing silk powder and condensed phosphate for the purpose of preventing coloring stains on teeth In order to improve the composition (Japanese Patent Laid-Open No. 2001-288061) and refreshing feeling after brushing, the average particle size is 50 to 1,000 μm with silk powder. Dentifrice compositions collapse strength were formulated in combination with particles that are 10 to 200 g / Pieces (JP 2002-255770) or the like is disclosed.

JP 2000-63251 A JP 2001-226243 A JP 2001-278757 A JP 2001-288061 A JP 2002-255770 A

  The present invention aims to provide an oral composition excellent in remineralization effect, which can effectively promote remineralization of decalcified enamel and thereby positively suppress caries. It is a thing.

The present invention uses silk powder, silk protein components sericin, fibroin, and / or a hydrolyzate thereof to promote remineralization of teeth, and these and calcium compounds including hydroxyapatite. It was found that the remineralization of the teeth was further promoted by the combined use, and the present invention was achieved.
The composition for oral cavity of the present invention is produced by adding the above-mentioned tooth enamel remineralization accelerator.

  Specifically, the present invention relates to an oral composition comprising silk powder, silk protein sericin, fibroin, and / or a hydrolyzate thereof, and silk powder, silk protein. An oral composition comprising a certain sericin, fibroin, and / or a hydrolyzate thereof, and a calcium compound such as hydroxyapatite is provided.

Silk powder is a powder of 70-80% fibroin and 30-20% sericin obtained by pulverizing silk obtained from silkworm cocoons.
These silk powder, silk protein sericin, fibroin, and hydrolysates thereof are obtained by a conventionally known method, and the production method is not limited.
Silk protein can be obtained as a silk extract by extracting silk fibers with dilute sulfuric acid solution.

  Various methods for obtaining fibroin have been proposed. Fibroin is a water-insoluble protein obtained by treating silk with hot water, cold water, hydrous alcohol, etc., and is taken out from a solution in urea and guanidine hydrochloride. be able to. Also disclosed is a method (Japanese Patent Laid-Open No. 2001-54359) for removing refined silk fibers from a dissolved neutral salt solution. A fibroin hydrolyzate can be obtained by hydrolyzing fibroin using various acids, alkalis and proteolytic enzymes.

  Sericin is a water-soluble protein obtained by treating silk with hot water, cold water, hydrous alcohol, etc., and hydrolyzed using alkali or a proteolytic enzyme to obtain a hydrolyzate of sericin.

  The silk powder, silk protein and hydrolyzate thereof are dissolved or dispersed in an appropriate liquid, or mixed with an appropriate powder carrier or adsorbed thereto. It is also possible to add a suspending agent, spreading agent, penetrating agent, wetting agent or stabilizer, etc. and formulate it into an emulsion, wettable powder, powder or tablet and use it.

  Calcium compounds are powders made by crushing natural hard tissues such as bones and eggshell shells of fish, amphibians, reptiles, birds or mammals that live in seawater or fresh water, calcium chloride, calcium carbonate, calcium lactate, gluconic acid Calcium, hydroxyapatite, tricalcium phosphate, calcium citrate, calcium hydrogen phosphate, calcium hydrogen phosphate dihydrate, calcium oxide and the like can be mentioned, and hydroxyapatite which is the same component as the tooth is particularly preferable.

  Hydroxyapatite crystals constituting the enamel on the tooth surface are composed of calcium phosphate and are considered to be effective as a source of calcium and phosphoric acid. In addition to calcium compounds synthesized in a normal manner, the natural hard tissue includes those containing abundant calcium compounds in the present invention. Examples include edible fish bones, pork bones, and beef bones.

  By using the above-mentioned silk powder, oral protein composition containing silk protein and its hydrolyzate, silk powder, silk protein, oral composition using hydrolyzate and calcium compound in combination, remineralization is remarkably achieved. The knowledge that it can be promoted was obtained.

  In silk powder, although good results can be obtained as a remineralization accelerator for tooth enamel, problems on the product such as discoloration due to various proteins in silk and off-flavors still remain. In commercialization, sericin, fibroin, and hydrolysates thereof, which are free from such problems, are particularly suitable, and sericin that is highly effective when used in a small amount. Moreover, as a calcium compound used together, hydroxyapatite is particularly suitable.

Examples of the oral composition of the present invention include toothpastes such as toothpaste, toothpaste and liquid toothpaste, mouthwash, 3DS preparation gingival masage cream, gargle tablets, troches and the like.
The addition amount of silk powder, silk protein and its hydrolyzate to the oral composition, and the addition amount of the calcium compound are difficult to determine in general depending on the form of the oral composition, but 0.001 to 50.0% by weight, preferably 0.01% to 50.0% by weight.
Furthermore, it is of course possible to blend not only one kind but also two or more kinds of silk powders and silk proteins. Similarly, not only one kind but also two or more kinds of calcium compounds can be blended.

In the present invention, as a method of adding silk powder, silk protein or a hydrolyzate thereof to the oral composition, it may be added at any time during the production process of the product, and the remaining raw materials may be mixed.
As a method of adding silk powder, silk protein or a hydrolyzate thereof, and a calcium compound to the oral composition, it may be added at any time during the production process of the product, and the remaining raw materials may be mixed. In addition, in the production of an oral composition to which silk powder, silk protein or a hydrolyzate thereof, and further a calcium compound are added, they may be mixed in advance, or separately, that is, silk powder, silk You may add protein, its hydrolyzate, and a calcium compound separately, respectively.

  The oral composition of the present invention is used in the form of a dentifrice such as a toothpaste, a liquid dentifrice, a powder dentifrice, a mouthwash, a 3DS preparation gingival masage cream, a gargle tablet, a troche and the like. obtain. The oral composition of the present invention can contain additives, humectants, foaming agents, fragrances, sweeteners, preservatives and various active ingredients that are commonly used in dentifrice compositions. Specific examples of these components are shown below.

Abrasive: calcium carbonate, calcium hydrogen phosphate, calcium pyrophosphate, etc. Wetting agent: glycerin, propylene glycol, polyethylene glycol and the like.
Foaming agent: sodium lauryl sulfate, sodium N-lauroyl sarcosine, nonionic surfactant and the like.
Thickener: hydroxyethylcellulose, sodium carboxymethylcellulose, carrageenan, carboxyvinyl polymer, chitansan gum and the like.
Preservatives: paraoxybenzoic acid ester, alkyldiaminoethylglycine hydrochloride, methylparaben, ethylparaben, sodium benzoate and the like.
Perfume: menthol, spearmint oil, lemon oil, eucalyptus oil, etc.
Sweetening agents: sodium saccharin, stevia extract, aspartame, etc.
Other medicinal ingredients: allantoin, tocopherol acetate, isopropylphenol, β-glycyrrhetinic acid, triclosan, chlorhexidine, dextranase, chlorophyll, flavonoid, tranexamic acid, hinokitiol and the like.

In addition, the compounding quantity of these arbitrary components is used suitably in the range accept | permitted pharmacologically, without preventing the effect of this invention.
EXAMPLES Examples and test examples of the present invention will be described below, but the following examples describe examples used for remineralization tests, and the scope of the present invention is not limited thereby. .

  The recalcification accelerator of the present invention has silk powder, sericin, fibroin and hydrolysates thereof, and silk powder, sericin, fibroin and hydrolysates thereof, and calcium compounds such as hydroxyapatite as active ingredients, The composition for oral cavity of the present invention produced by adding the remineralization accelerator effectively promotes the remineralization of the demineralized tooth enamel and actively suppresses caries. Is advantageous.

It is a photograph which shows the control surface and processing surface of a crown part by a contact microradiogram (CMR). FIG. 2 is a diagram in which the photograph shown in FIG. 1 is drawn using dark ink and a description of the state of each part is added.

  Hereinafter, the present invention will be described in detail with reference to examples.

[Recalcification promotion test method]
In order to confirm the effect of promoting remineralization, an artificial initial caries test sample prepared in advance was used.
The artificial caries test sample was prepared by using the crown of a human extracted tooth that had not undergone dental preservation and prosthesis treatment, removing the dirt and deposits on the enamel surface, and then testing the enamel surface. A target area of 3.5 × 3.0 mm is created with Nail Enamel and is placed in 0.1 M lactate buffer (pH 4.5, 3.0 mm CaCl ₂ , 1.8 mm KH ₂ PO ₄ , 0.5% CMC) 37 An artificial initial caries was created by dipping at 7 ° C. for 7 days. For control of the test, half of the crown top portion side of the 3.5 × 3.0 mm window was further masked with Nail Enamel, and used as a comparison target site (control).
A test solution was prepared by suspending dentifrice, liquid dentifrice, liquid dentifrice, and mouthwash into a suspension solution with artificial saliva.

  In the remineralization acceleration test, the artificial initial caries test specimen prepared above was immersed in each test solution for 24 hours, and then the specimen was about 500 μm thick so as to be parallel to the tooth axis with a microcutter. After cutting, this piece was polished using a kneading stone and a natural whetstone so as to obtain a parallel thin piece having a thickness of about 100 μm under water injection. After polishing, contact microradiogram (CMR) was taken to confirm the effect of remineralization of teeth (see Fig. 1 and Fig. 2). Analysis.

  Image analysis on a computer is based on the formula of Angmer et al. -23, 1963), and the amount of mineral remineralized is calculated, and the method of Damato et al. (FA Damato, R. Stang and KW Stephen: Effect of Fluoride Concentration on Regenerative of Carriage: in vitro pH-Cycling Stu y, Caries Res, according 24,174-180,1990) was calculated mineral loss values ΔZ (% volume mineral · μm) of the control surface and the treated surface of each section. In addition, the remineralization rate was computed by the following formula | equation.

Table 5 shows the results of confirming the promotion effect of remineralization by the above-described remineralization promotion test method.
The calcification rate when using a hydrolyzate of sericin was the same as that of sericin.

“Control surface” in the figure is a part for comparing the degree of remineralization effect of “oral compositions” in Examples and Comparative Examples. The state is maintained. It is a half of the artificial initial caries (3.5 × 3.0 mm window) region.
The “processing surface” in the figure is a portion where the specimens of the example and the comparative example are operated as follows.
(1) The test substance was prepared by using a suspension solution of a dentifrice or mouthwash and artificial saliva as a test solution.
(2) In the remineralization promotion test, the artificial initial caries test specimen was immersed in each test solution for 24 hours.

Claims (4)

A composition for promoting remineralization of tooth enamel , which is produced by adding sericin and / or a hydrolyzate thereof. Characterized in that blended with the calcium compound, according to claim 1 oral composition. Calcium compounds are natural bone tissue powder, calcium chloride, calcium lactate, calcium gluconate, hydroxyapatite, tricalcium phosphate, calcium citrate, calcium hydrogen phosphate, calcium hydrogen phosphate dihydrate, calcium oxide The composition for oral cavity of Claim 2 formed by mix | blending 1 type (s) or 1 or more types of these . A method for producing a composition for promoting oral remineralization of tooth enamel, comprising adding sericin and / or a hydrolyzate thereof.

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