JP5044166B2 - Method for producing extract of stem and / or branch of Ekokogi - Google Patents
Method for producing extract of stem and / or branch of Ekokogi Download PDFInfo
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Description
本発明は、あまり薬効の期待されないエゾウコギの茎や枝から有効成分を抽出した抽出物、その製造方法及びその用途に関するものである。 The present invention relates to an extract obtained by extracting an active ingredient from a stem or a branch of Ezokogi, which is not expected to have a medicinal effect, a method for producing the same, and a use thereof.
エゾウコギ（学名：Acanthopanax senticosus harms）は、ウコギ科ウコギ属に属する植物である。エゾウコギには滋養強壮作用、鎮静作用、抗炎症作用、鎮痛作用、解熱作用等の薬理作用があることが公知である。 Ezoukogi (scientific name: Acanthopanax senticosus harms) is a plant belonging to the genus Uguigiaceae. It is known that Ezokogi has pharmacological actions such as nourishing tonicity, sedation, anti-inflammatory action, analgesic action, and antipyretic action.
特に、エゾウコギの根の抽出物は、薬効が高く薬事法で認可されている（非特許文献１）。 In particular, the extract of root of Ekokogi has a high medicinal effect and is approved by the Pharmaceutical Affairs Law (Non-patent Document 1).
エゾウコギの根の抽出物には、エレウテロサイドB、イソフラキシジン、エレウテロサイドB1、エレウテロサイドE等の多糖類が含まれており、これらの作用により上述の薬理作用を奏するものと考えられているが詳細は不明である。 The extract of root of Ezoukogi contains polysaccharides such as eleuteroside B, isofraxidine, eleuteroside B1 and eleuteroside E, and these actions are considered to exert the above-mentioned pharmacological action. However, details are unknown.
エゾウコギの根以外の茎や枝から抽出した抽出物中の多糖類の組成は、根の抽出物の多糖類の組成と異なっており、茎や枝の抽出物は根の抽出物と比較すると薬効が劣っており、健康補助食品として使用されている。
本発明が解決しようとする課題は、薬効の期待されないエゾウコギの茎や枝から有効成分を抽出した抽出物、その製造方法及びその用途を提供することにある。 The problem to be solved by the present invention is to provide an extract obtained by extracting an active ingredient from the stem or branch of Ezokogi, which is not expected to have a medicinal effect, its production method, and its use.
上記課題を解決するため、鋭意研究を重ねたところ、本発明者は特定の抽出溶媒でエゾウコギの茎や枝を抽出することにより課題を解決できることを見出し、本発明を完成するに至った。 As a result of diligent research to solve the above problems, the present inventor has found that the problems can be solved by extracting stems and branches of elephants with a specific extraction solvent, and the present invention has been completed.
すなわち本発明は下記に関するものである。
（１） エゾウコギの茎及び／又は枝をアルカリ電解水で抽出することを特徴とするエゾウコギ抽出物の製造方法。
That is, the present invention relates to the following.
(1) A method of manufacturing features and to Rue Zoukogi extract that the stems and / or branch of Acanthopanax extract with alkaline electrolyzed water.
本発明のエゾウコギの茎及び／又は枝からアルカリ電解水で抽出した抽出物は、薬効が高く、ダイエット用のサプリメントや飲食品として好適である。 The extract extracted with alkaline electrolyzed water from the stems and / or branches of Ezoukogi of the present invention has high medicinal effects and is suitable as a dietary supplement or food and drink.
本発明のエゾウコギの茎及び／又は枝からアルカリ電解水で抽出した抽出物が、ダイエット効果を奏することの詳細な理由は不明である。 The detailed reason why the extract extracted with alkaline electrolyzed water from the stem and / or branch of the present invention has a diet effect is unknown.
本発明の原料となるエゾウコギの茎や枝は、エゾウコギの茎や枝を乾燥し、裁断したものを用いればよい。 As the stems and branches of the Ekokogi used as the raw material of the present invention, what is obtained by drying and cutting the stems and branches of Ezoukogi may be used.
本発明の抽出溶媒は、アルカリ電解水である。本発明におけるアルカリ電解水とは、
隔膜でアノード室とカソ−ド室に仕切られた隔膜電解槽に水を供給してカソ−ド電解により生成したアルカリ性の電解水のことである。
The extraction solvent of the present invention is alkaline electrolyzed water. With alkaline electrolyzed water in the present invention,
This is alkaline electrolyzed water produced by cathodic electrolysis by supplying water to a diaphragm electrolyzer partitioned by a diaphragm into an anode chamber and a cathode chamber.
アルカリ電解水で抽出するための条件は、例えば、逆浸透膜処理水をカソ−ド電解して
生成したアルカリ性電解水に細断したエゾウコギの茎及び枝を浸漬した後、アルカリ性電解水を加熱して抽出すればよい。
The conditions for extraction with alkaline electrolyzed water are, for example, by immersing sorghum stems and branches in alkaline electrolyzed water produced by cathodic electrolysis of reverse osmosis membrane treated water, and then heating the alkaline electrolyzed water. To extract.
アルカリ電解水でエゾウコギの茎や枝を抽出した抽出物中のエレウテロサイドB、イソフラキシジン、エレウテロサイドB1、エレウテロサイドEの組成は、従来法で抽出したエゾウコギの茎や枝の抽出物の組成と異なり、むしろ根の抽出物の組成と近いものである（図１参照）。エゾウコギの茎及び／又は枝のアルカリ電解水抽出物のエレウテロサイドB、イソフラキシジン、エレウテロサイドB1、エレウテロサイドEの組成は、各々０.０１〜０．３、０.０１〜０．２、０．０１〜０．３、０．１〜０．５の範囲にある。 The composition of eleuteroside B, isofraxidine, eleuteroside B1 and eleuteroside E in the extract obtained by extracting sorghum stalks and branches with alkaline electrolyzed water is the extraction of sorghum stalks and branches extracted by conventional methods. Unlike the composition of the product, it is rather close to the composition of the root extract (see FIG. 1). The composition of eleuteroside B, isofraxidine, eleuteroside B1 and eleuteroside E in the alkaline electrolyzed water extract of sorghum stem and / or branch is 0.01 to 0.3 and 0.01 to 0, respectively. .2, 0.01 to 0.3, 0.1 to 0.5.
本発明の抽出物は、エゾウコギの茎や枝を抽出したままの液状でも良く、抽出液を濃縮したコンク品でもよく、抽出液を乾燥した粉末品でもよい。 The extract of the present invention may be in the form of a liquid obtained by extracting stems and branches of elephants, may be a concentrated product obtained by concentrating the extract, or may be a powder product obtained by drying the extract.
本発明の抽出物は、凍結乾燥して、粉末化し、リン酸カルシウム、セルロース、アルギン酸、ステアリン酸カルシウム、ショ糖等と配合してサプリメントとすることができる。 The extract of the present invention can be freeze-dried, powdered, and blended with calcium phosphate, cellulose, alginic acid, calcium stearate, sucrose, etc. to make a supplement.
本発明のサプリメントは後述の実施例から分かるように、ダイエット用のサプリメントとして好適である。 The supplement of the present invention is suitable as a dietary supplement, as can be seen from the examples described later.
本発明の抽出物は、飲料水、ドリンク、茶の飲料やビスケットのような食品に配合することにより、ダイエット効果が期待できる。
参考例１
実施例で使用したアルカリ電解水は、以下のように調製した。まず、図１に示す電解槽を用いて、逆浸透膜処理水をアノード室１とカソ−ド室６に供給して、pHが〜10の水を生成した。
The extract of the present invention can be expected to have a diet effect by blending it into foods such as drinking water, drinks, tea beverages and biscuits.
Reference example 1
The alkaline electrolyzed water used in the examples was prepared as follows. First, using the electrolytic cell shown in FIG. 1, reverse osmosis membrane treated water was supplied to the anode chamber 1 and the cathode chamber 6 to generate water having a pH of ˜10.
(1)抽出
乾燥エゾウコギ原木（茎主体）を針状に切断し、25ｇに対して溶媒250ｇを加え、80〜100℃にて溶媒抽出を行った。それぞれ室温まで冷却後、濾紙にてろ過し、抽出液量、エキス濃度、比重、pHを測定した。抽出液量とエキス濃度より抽出率（100ｇより抽出された乾燥エキスのｇ数）を求めた。抽出条件と測定結果を表１に示す。
(1) Extraction Dried Sorghum logs (mainly stems) were cut into needles, 250 g of solvent was added to 25 g, and solvent extraction was performed at 80 to 100 ° C. Each was cooled to room temperature, filtered through filter paper, and the amount of extract, extract concentration, specific gravity, and pH were measured. The extraction rate (number of g of dry extract extracted from 100 g) was determined from the amount of the extract and the concentration of the extract. Table 1 shows extraction conditions and measurement results.
（２）成分分析
抽出物を液体クロマトグラフ法によりエゾウコギの特徴5成分を測定した。測定結果を表２に示す。
(2) Component analysis The extract was measured for five characteristic components of Ekogigi by liquid chromatography. The measurement results are shown in Table 2.
表２中、「通常水抽出１」とは電解無処理の水を80〜100℃に加熱して抽出した例を示す。
意味し、「通常水抽出２」とは熱水抽出1と同様な条件で抽出した別の例を意味する。
In Table 2, “normal water extraction 1” refers to an example in which electrolytically untreated water is extracted by heating to 80 to 100 ° C.
Meaning, “normal water extraction 2” means another example extracted under the same conditions as hot water extraction 1.
表２の測定結果を、クロロゲン酸を１とした場合のエレウテロサイドB、イソフラキシジン、エレウテロサイドB1、エレウテロサイドEの重量比をグラフ化した（図２）。グラフ中「根」とあるのは、エゾウコギの根を熱水抽出した抽出物を測定したものである。 The measurement results in Table 2 are graphed based on the weight ratio of eleuteroside B, isofraxidine, eleuteroside B1, and eleuteroside E when chlorogenic acid is 1. (FIG. 2) The “root” in the graph is a measurement of an extract obtained by hot water extraction of the root of Ezokogi.
表２及び図２の結果から明らかなように、本発明のエゾウコギの茎のアルカリ電解水抽出物の有効成分の組成は、従来の熱水抽出物の組成と異なり、むしろ根の熱水抽出物の組成に近いものであった。 As is apparent from the results of Table 2 and FIG. 2, the composition of the active component of the alkaline electrolyzed water extract of the sorghum stalk of the present invention is different from the composition of the conventional hot water extract, rather the hot water extract of the root. It was close to the composition.
本実施例では、アルカリ電解水抽出エゾウコギエキスの抗肥満効果を様々なパラメータから検証を行い、既存の水抽出エゾウコギエキスの抗肥満効果との違いを明らかにする。 In this example, the anti-obesity effect of alkaline electrolyzed water extract Ekokogi extract is verified from various parameters, and the difference from the anti-obesity effect of existing water-extracted Ezoukogi extract is clarified.
効果確認試験には以下の動物を用いた。 The following animals were used for the effect confirmation test.
a.使用動物
SD雄性ラット４週令を購入後、１週間の予備飼育を行い、実験に使用した。
a. Animals used
After purchasing SD male rats 4 weeks old, they were preliminarily raised for 1 week and used for experiments.
b.グループ
・H2O,ND：普通食(360 kcal/100g)自由摂取ラット ６匹（図中、「普通食」と表示）
・H2O,HFD35%：βカゼイン5%入り35%ラード入り高脂肪(630 kcal/100g)食自由摂取ラット ６匹（図中、「高脂肪食」と表示）
・AlkaliASH5%,HFD：アルカリ電解水抽出エゾウコギエキス5%入り高脂肪食(630 kcal/100g)自由摂取ラット ６匹（図中、「電解水抽出」と表示）
・ASH5%,HFD：普通の水抽出エゾウコギエキス0.5%入り高脂肪食(630 kcal/100g)自由摂取ラット ６匹（図中、「通常水抽出」と表示）
b. Group H2O, ND: Normal diet (360 kcal / 100g) 6 rats with free intake (indicated as “normal diet” in the figure)
・ H2O, HFD35%: β casein 5% containing 35% lard containing high fat (630 kcal / 100g) diet free rats 6 (indicated as “high fat diet” in the figure)
・ AlkaliASH5%, HFD: Alkaline electrolyzed water extract 5% high-fat diet (630 kcal / 100g) containing 5% Ezokogi extract (indicated as “electrolyzed water extract” in the figure)
・ ASH5%, HFD: Normal water extracted Ezokogi extract 0.5% high fat diet (630 kcal / 100g) free intake 6 rats (indicated as “normal water extraction” in the figure)
これらのラットを予備飼育後、５週間、それぞれの処置を行った。 Each of these rats was treated for 5 weeks after preliminary breeding.
試験結果は以下のとおりまとめられる。 The test results are summarized as follows.
（１）アルカリ電解水抽出エゾウコギエキスの体重増加率への影響（図３）
高脂肪食投与ラットは、普通食投与ラットに比べ１日あたり平均約1.9倍の体重増加が認められた。高脂肪食条件下、通常の水抽出ASHエキスは、高脂肪食単独投与ラットに比べ１日あたり平均約27%の体重抑制を示した。一方、アルカリ電解水抽出エキスは、高脂肪食単独投与ラットに比べ１日あたり平均約42-49%の体重抑制を示し、アルカリ電解水抽出エキスによる作用の増強効果が明らかとなった。
(1) Effects of alkaline electrolyzed water-extracted Ekogi extract on body weight gain (Figure 3)
Rats administered with a high-fat diet showed an average weight gain of about 1.9 times per day compared with rats administered with a normal diet. Under high-fat diet conditions, the normal water-extracted ASH extract showed an average weight reduction of about 27% per day compared to rats administered with a high-fat diet alone. On the other hand, the alkaline electrolyzed water extract showed an average body weight suppression of about 42-49% per day as compared with the high-fat diet alone-administered rats.
（２）アルカリ電解水抽出エゾウコギエキスの内臓脂肪への影響（図４）
高脂肪食投与ラットは、普通食投与ラットに比べ約2.7内臓脂肪組織重量の増加が認められた。高脂肪食条件下、通常の水抽出ASHエキスは、高脂肪食単独投与ラットに比べ約26％脂肪重量の低下を示した。一方、アルカリ電解水抽出エキスは、高脂肪食単独投与ラットに比べ約52%の低下作用を示した。アルカリ電解水抽出エキスによる作用の増強効果が明らかとなった。
(2) Effects of alkaline electrolyzed water-extracted sorghum extract on visceral fat (Figure 4)
About 2.7 visceral adipose tissue weight was increased in the high fat diet administered rats compared to the normal diet administered rats. Under high-fat diet conditions, the normal water-extracted ASH extract showed a 26% reduction in fat weight compared to the high-fat diet-only rats. On the other hand, the alkaline electrolyzed water extract showed an approximately 52% lowering effect compared to the high fat diet-only rats. The enhancement effect of alkaline electrolyzed water extract was clarified.
（３）血中中性脂肪値に対するアルカリ電解水抽出エゾウコギエキスの効果（図５）
ラットへの高脂肪食投与は、血中中性脂肪値が、普通食に比較して上昇傾向を示した。アルカリ電解水抽出エキスは、高脂肪食による血中中性脂肪値の上昇を顕著に抑制し、さらに、通常水抽出エキスに比較しても抑制した。
(3) Effect of alkaline electrolyzed water-extracted Ekogigi extract on blood neutral fat level (Fig. 5)
Administration of a high-fat diet to rats showed an increase in blood triglyceride levels compared to the normal diet. The alkaline electrolyzed water extract significantly suppressed an increase in blood neutral fat level due to a high-fat diet, and further suppressed it compared to a normal water extract.
（４）血糖値に対するアルカリ電解水抽出エゾウコギエキスの効果（図６）
高脂肪食による血糖値の上昇は、アルカリ電解水抽出エキスや水抽出ASH5%エキスにより顕著に抑制された。また、アルカリ電解水抽出エキスは、ASH5%エキスの効果をさらに増強していることも明らかとなった。
(4) Effect of alkaline electrolyzed water-extracted Ekogi extract on blood glucose level (Fig. 6)
The increase in blood glucose level due to a high fat diet was remarkably suppressed by alkaline electrolyzed water extract or water-extracted ASH5% extract. It was also revealed that the alkaline electrolyzed water extract further enhanced the effect of the ASH 5% extract.
本発明のエゾウコギの茎や枝の抽出物は、ダイエット効果が期待できるので、ダイエット用のサプリメントや飲食品として有用である。 Since the extract of the stem and branch of Ekokogi of the present invention can be expected to have a diet effect, it is useful as a dietary supplement or food and drink.
Claims (1)
Method of manufacturing features and to Rue Zoukogi extract that the stems and / or branch of Acanthopanax extract with alkaline electrolyzed water.
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| KR101011285B1 (en) * | 2010-04-23 | 2011-01-28 | 변경삼 | Pharmaceutical composition for lowering back disc pain and method for preparing same |
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| JP2017007974A (en) * | 2015-06-19 | 2017-01-12 | 株式会社サン・クロレラ | Cardiac function regulatory agent |
| TW201900037A (en) * | 2017-03-31 | 2019-01-01 | 日商太陽綠藻股份有限公司 | Method for preparing learning effect improving agent and food composition for improving learning effect |
| TW201842927A (en) * | 2017-03-31 | 2018-12-16 | 日商太陽綠藻股份有限公司 | Method for producing anxiolytic drug and anxiolytic food product composition, and technology pertaining thereto |
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