JP5124665B2 - Fine object collection tool and method for collecting and identifying minute object using the same - Google Patents

Description

  The present invention relates to a fine collection tool for collecting fine materials such as fiber scraps, paint film pieces, DNA type identification data, etc. present in crime scenes and the like, and a method for collecting and identifying fine materials using the same.

In recent years, crimes have become vicious and sophisticated, and minute inspections that are collected at criminal scenes etc. are identified as important inspection activities for identifying criminals and verifying crimes. . In addition, DNA type appraisal that examines DNA polymorphic regions by utilizing the fact that there are different parts in individual DNA has made it possible to identify individuals with high accuracy due to technological advances. For this reason, the importance of the crime as crime is increasing, the implementation status is increasing year by year, and it is important to collect DNA type identification materials at crime scenes.
However, when performing fine object identification, it may be difficult to make an appraisal if the amount of fine objects collected is not sufficient. In addition, if a foreign object is mixed when collecting a fine object, a correct appraisal result may not be obtained or appraisal may be difficult. In particular, DNA type identification is difficult to determine if other people's materials are mixed into the material to be collected. Therefore, it is required to collect fines by collecting a sufficient amount of fines and preventing foreign matters from being mixed.

  Conventionally, collection of fine objects has been performed using a commercially available adhesive tape or a re-tack sheet. There are also suggestions for collecting fines. For example, there is a fine object adsorption collection device that adsorbs and collects fine objects such as fiber pieces, hair, earth and sand by static electricity (see Patent Document 1). Further, there is a fine collection film in which a pressure-sensitive adhesive layer having a weak adhesive force is provided on the back surface of a transparent synthetic resin sheet that is likely to generate static electricity and is charged, and a release paper is polymerized on the pressure-sensitive adhesive layer (see Patent Document 2). ).

JP 2009-240101 A Japanese Utility Model Laid-Open No. 1-137452

  However, when collecting fines with a commercially available adhesive tape or retuck sheet, it is not possible to collect a sufficient amount of fines because of its low tackiness, and the ability to follow the uneven surface of the collection point is low Depending on the form of the sampling location, a sufficient amount of sampling could not be collected. In addition, foreign substances may be mixed into the adhesive surface, especially when collecting DNA type identification materials, even if it is used with extreme caution, other people's materials may be mixed. Since the fine matter sticking to the adhesive tape or the re-tack sheet is difficult to peel off, complicated work is required when the fine matter is peeled off and appraisal equipment such as a microscope is used. On the other hand, since the fine object collection device described in Patent Document 1 and the fine material collection film described in Patent Document 2 collect fine objects by static electricity, they are not affected by the form of the collection point of the fine objects. Even if it was able to be collected, there was a case where foreign matters were easily mixed. It has been difficult for the fine substance adsorption collection device described in Patent Document 1 and the fine sample collection film described in Patent Document 2 to collect DNA type identification materials as fine objects. The fine matter adsorbing and collecting apparatus described in Patent Document 1 requires troublesome setting of the apparatus, and the fine object collecting film described in Patent Document 2 generates static electricity by peeling off the release paper and causing static electricity in the sheet. Because it is necessary to make them work, it is difficult to handle them unless they are specialists.

  The present invention has been made in view of the above circumstances, and can be easily and quickly sampled by preventing foreign matter from entering a sufficiently collected amount of fines without being influenced by the form of the collection point of the fines. It is an object of the present invention to provide a tool for collecting fine objects and a method for using the same to easily and quickly collect and test fine objects.

  In order to solve the above-mentioned problems, the present inventors have made extensive studies and completed the present invention. That is, the present invention is a fine material collection tool for adhering and collecting fine materials present in crime scenes and DNA type identification materials, and a gel sheet of an adhesive resin coated with a protective sheet is on one side A sampling part composed of a flat plate that is detachably attached to the container, a protection part composed of a box-shaped body with at least an open top, and an operation part capable of being gripped, and the sampling part and the protection part The collection part can be detachably attached to the protection part by attachment means provided in between, and when the collection part is attached to the protection part with the surface side to which the gel sheet is attached facing down, the protection part Is formed so that a gap is formed between the inner bottom surface and the upper surface of the gel sheet, and the protection portion is detachably attached to the operation portion by an attachment means provided between the protection portion and the operation portion. The protective part to which the sampling part is attached is attached to the operation part, and the fine part can be collected by operating the gripping part and sticking the fine part at the top of the gel sheet. The gist of the tool for collecting fine objects characterized by this is as follows. With this configuration, it is possible to simply, easily and quickly collect a sufficient amount of fine matter while preventing contamination by foreign matter, regardless of the form of the fine matter collection location. Here, the fine matter is a minute, fine, and minute amount present in crime scenes, other places that are recognized to be related to crimes, people and things, such as fiber scraps, film pieces, glass pieces, soil, This refers to materials used for DNA type identification such as pollen and seeds and saliva, blood, blood stains, body fluids, bones, tissue fragments, nails and hair.

  In the above invention, the attachment means provided between the sampling portion and the protection portion so that the attachment / detachment direction of the sampling portion with respect to the protection portion and the attachment / detachment direction of the protection portion with respect to the operation portion are orthogonal to each other, and You may provide the said attachment means provided between a protection part and the said operation part, respectively. With this configuration, when removing the collection unit from the protection unit, the protection unit is not detached from the operation unit along with the removal of the collection unit, and the removal of the collection unit from the protection unit can be easily performed.

  In each of the above inventions, the gel sheet may be a polyurethane gel sheet having a rubber hardness of 30 or less. With this configuration, the followability to the uneven surface of the collected portion of the adhesive portion is increased. Here, the followability to the uneven surface of the collecting part of the adhesive part refers to a property that the adhesive part is deformed by pressing according to the form of the uneven surface of the collecting part.

  In each of the above inventions, the fine material may be used as DNA type identification data or fiber waste. With this configuration, it is possible to obtain powerful evidence to prove a crime.

  The present invention is a method for collecting and appraising a fine object using the fine object collecting tool according to any one of claims 1 to 3, wherein (1) the fine object is an upper surface of the gel sheet of the collecting unit. And (2) a process of removing the sampling part from which the fine material has been collected with the protective part attached to the operating part from the protective part or removing the protective part from the operating part, and the sampling part Removing from the protective part, (3) attaching the sampling part to the protective part with the surface side on which the gel sheet is attached facing down, and (4) attaching the sampling part to the operating part in the process (2) When removing the sampling part from which the fines have been collected with the protective part attached from the protective part, removing the protective part to which the sampling part is attached from the operation part; and (5) the sampling part is The attached protective part A process of storing and storing in a container that can be closed; and (6) a process of removing the collection unit from the protection unit and observing the collection unit as a subject with a microscope, or removing the gel sheet from the collection unit and performing DNA analysis The gist of the method is the collection and appraisal of fine objects characterized by comprising: In the present invention, the fine substance may be DNA type identification data or fiber waste.

The present invention has the following effects.
(1) Since the gel sheet of the present invention has excellent adhesiveness and followability to the uneven surface of the collection point of the fine material, the gel collection tool of the present invention is not affected by the form of the fine material collection point, It is easy to collect a sufficient amount of fines even from places where it was difficult to collect. For example, in the case of a molester case, it is possible to collect tissue fragments even from clothes that have been difficult to collect data for DNA type identification. It is also possible to prove crimes by doing so, and a great contribution can be expected to solve the crimes.
(2) The tool for collecting fine objects of the present invention can collect fine objects by preventing foreign substances from being mixed, so that the accuracy of the identification can be improved, and it is particularly useful for DNA type identification, and is very effective in solving crimes. Can make great contributions.
(3) The tool for collecting fine objects of the present invention can be easily and quickly collected, and can also be collected by police officers who have been sent to incident sites other than specialists in charge of expert knowledge. Therefore, it can contribute to smooth fine article inspection, and in turn can be expected to contribute to the early resolution of crimes.
(4) The micro sample collection tool of the present invention is simple and easy to mix foreign substances into the gel sheet by collecting the micro sample after the micro sample is collected by simply attaching and collecting the sample unit to the protective unit with the side to which the gel sheet is attached facing down. And it can be prevented quickly.

The present invention has the following effects.
The method for collecting and appraising fines according to the present invention allows simple and easy and quick collection of fines, and can easily and quickly prevent foreign substances from being mixed into the collected fines, and can be stored. The part is composed of a flat plate, and it is not necessary to remove the gel sheet one by one and place it on the slide glass. Can be carried out simply, easily and quickly.

It is a front view of a collection part. It is a top view of a collection part. It is a front view of a protection part. It is a top view of a protection part. It is a side view of a protection part. It is a side view of an operation part. It is a top view of an operation part. It is a bottom view of an operation part. It is a top view of the protection part to which the collection part was attached. It is a front view of the fine material collection tool with which the protection part to which the collection part was attached was attached to the operation part. It is a side view of the fine object collection tool with which the protection part to which the collection part was attached was attached to the operation part. It is a perspective view of the fine material collection tool with which the protection part to which the collection part was attached was attached to the operation part. It is a front view of the protection part where the collection part was attached by making the surface side to which a gel sheet is attached down. It is a top view of the protection part attached so that the surface side to which a gel sheet is attached to the collection part may be down.

  Next, the present invention will be described with reference to embodiments, but the present invention is not limited to the following embodiments.

  As shown in FIGS. 10 to 12, the fine object collecting tool 100 according to the present embodiment includes a collection unit 10, a protection unit 20, and an operation unit 30.

  As shown in FIGS. 1 and 2, the collection unit 10 includes a substantially rectangular flat plate 15 made of polystyrene. Protrusions 11 are formed at opposite positions on the side surface of the flat plate 15. Further, a step portion 12 is formed on the flat plate 15, and a transparent adhesive resin gel sheet 13 (polyurethane gel sheet, manufactured by EXCIAL Corporation (gel tack sheet)) is attached to the step portion 12 by adhesion. The upper surface of the gel sheet 13 is covered with a protective sheet 14 formed of a transparent polyethylene film. The protective sheet 14 is formed in a size larger than the area of the upper surface of the gel sheet 13, and can be easily detached from the gel sheet 13 using a portion protruding from the gel sheet 13. The protective sheet 14 is not limited to the above polyethylene film as long as it can be covered on the upper surface of the gel sheet 13 and can be removed when collecting fines. Examples of protective sheets made of other materials include various resin films such as polypropylene, polyester, and nylon, and various paper materials such as polyethylene laminated paper, crecote paper, and glassine paper.

  The gel sheet 13 is a polyurethane composition in which an alcohol component and an organic polyisocyanate are mixed. When the total amount of the alcohol component is 100 parts by weight, the number of functional groups is 2.4 to 3.0, and the molecular weight is 3. A polyurethane composition containing 99.5 to 90 parts by weight of a 1,000 to 6,000 polyol and 0.5 to 10 parts by weight of a secondary or tertiary higher monoalcohol has a rubber hardness of 30 or less. It is a polyurethane gel sheet and is easily available. This gel sheet 13 is excellent in adhesiveness when collecting fines and peelability of the fines after collection, and since it is flexible, it is excellent in following the uneven surface of the collection point of fines. Moreover, since the plasticizer is not added to the gel sheet 13, the influence of the plasticizer on the identification can be avoided particularly in the DNA type identification.

  In the case of DNA type identification in which the gel sheet 13 is immersed in a test solution and DNA is extracted, it is necessary to remove the gel sheet 13 from the step portion 12. In this case, if the removal of the gel sheet 13 from the stepped portion 12 is easy, it can contribute to speeding up the appraisal. Therefore, the adhesive strength of the lower surface of the gel sheet 13 is attenuated more than the adhesive strength of the upper surface of the gel sheet 13 for collecting fines. You may let them. As a method for reducing the adhesive strength, for example, a resin film can be attached in advance to the lower surface of the gel sheet 13 and an adhesive having a weak adhesive strength can be applied to the resin film.

  The gel sheet 13 is not limited to the above-mentioned polyurethane gel sheet as long as it is an adhesive resin gel sheet. Examples of other adhesive resin gel sheets include silicon gel sheets, polystyrene gel sheets, acrylic gel sheets, and polyvinyl chloride gel sheets.

  As shown in FIGS. 3-5, the protection part 20 is comprised from the substantially square box-shaped body 25 by the planar view formed by the polystyrene which the upper part and the front part opened. A mounting groove 21 is provided around the upper edge of the protection portion 20. In addition, through holes 22 are formed at opposing positions on both sides of the mounting groove 21. Since the sampling unit 10 is configured by the flat plate 15, the sampling unit 10 can be detachably attached to the protection unit 20 by inserting the sampling unit 10 into the mounting groove 21 of the protection unit 20. When attached, since the projection 11 of the sampling part 10 is fitted into the through hole 22 of the protection part 20, it is possible to prevent the sampling part 10 from being easily detached. An attachment step portion 23 is formed in the lower portion of the protection portion 20. Further, a protrusion 24 is formed on the lower surface of the mounting step portion 23. As shown in FIG. 13, when the collection unit 10 is attached to the protection unit 20 with the surface on which the gel sheet 13 is attached facing down, the protection unit 20 has a gap S between its inner bottom surface and the upper surface of the gel sheet 13. It is formed to be able to.

  As shown in FIGS. 6 to 8, the operation unit 30 is made of polypropylene, and includes a hollow elliptical columnar body 36 having a mounting base 31 on the upper part. On the outside of the elliptical columnar body 36, a number of anti-slip ridges 32 are provided. A large number of reinforcing ribs 33 are provided around the elliptical columnar body 36. A mounting groove 34 is provided around the mounting base 31. A through hole 35 is formed near one end of the mounting base 31. As shown in FIG. 9, the protective portion 20 can be detachably attached to the operation portion 30 by inserting the attachment step portion 23 of the protective portion 20 into the attachment groove 34, and when the protective portion 20 is attached, Since the protrusion 24 of the protection part 20 fits into the through hole 35 of the operation part 30, it is possible to prevent the protection part 20 from being easily detached.

  As shown in FIGS. 3 to 6 and 11, the mounting groove 21 of the protection unit 20, the protection unit so that the attaching / detaching direction of the sampling unit 10 with respect to the protection unit 20 and the attaching / detaching direction of the protection unit 20 with respect to the operation unit 30 are orthogonal to each other. 20 mounting steps 23 and mounting grooves 34 for the operation unit 30 are formed.

  The materials of the collection unit 10, the protection unit 20, and the operation unit 30 are not limited to the above materials, and materials other than resin can also be used. In the case of a resin, various resins can be used, and examples thereof include polyethylene, polyolefin, polyvinyl chloride, and polyester.

  As shown in FIGS. 10 to 12, in the fine object collecting tool 100 according to the present embodiment, the protection unit 20 to which the collection unit 10 is attached is attached to the operation unit 30, and the collection unit 10, the protection unit 20, and the operation unit are attached. 30 is integrated, and the ellipsoidal columnar body 36 of the operation unit 30 is gripped and operated, and the gel sheet 13 of the collection unit 10 is pressed against the fine object at the collection location, whereby the collection of the fine object can be performed easily and easily. it can. The fine sampling tool 100 is stored in advance in a storage bag (not shown) so that foreign matter is not mixed. In addition, the storage bag can be accommodated individually without the collection unit 10, the protection unit 20, and the operation unit 30 being integrated. In order to prevent foreign matters from being mixed into the gel sheet 13, the operation of attaching the gel sheet 13 to the sampling unit 10 and the operation of covering the upper surface of the gel sheet 13 with the protective sheet 14 are performed in a clean room with an air shower.

Hereinafter, a method for collecting and appraising a fine object using the fine object collecting tool 100 according to the present embodiment will be described. At the sampling site, the fine sampling tool 100 in which the sampling unit 10, the protection unit 20, and the operation unit 30 are integrated is taken out from the storage bag. When the collection unit 10, the protection unit 20, and the operation unit 30 are individually stored in the storage bag without being integrated, the collection unit 10 and the protection unit 20 are attached at the collection site. Next, the protective sheet 14 is removed from the gel sheet 13, the operation unit 20 is gripped, pressed against the fine material at the collection location, and adhered to the gel sheet 13 to collect the fine material. After collection of the fine objects, the collection unit 10 is removed from the protection unit 20, and the collection unit 10 is attached to the protection unit 20 with the surface side to which the gel sheet 13 is attached facing down, as shown in FIGS. Since the attachment / detachment direction of the collection unit 10 with respect to the protection unit 20 and the attachment / detachment direction of the protection unit 20 with respect to the operation unit 30 are orthogonal to each other, when removing the collection unit 10 from the protection unit 20, protection is performed from the operation unit 30 along with the removal of the collection unit 10. The part 20 is not detached, and the sampling part 10 can be easily detached from the protection part 20. There is a gap S between the inner bottom surface of the protection unit 20 and the upper surface of the gel sheet 13, and the sampling unit 10 is accommodated with the upper surface of the gel sheet 13 to which the fine substance adheres facing downward. The upper surface of the gel sheet 13 to which an object is adhered is protected, and foreign substances can be prevented from being mixed with the upper surface of the gel sheet 13. Further, the removal of the collection unit 10 from the protection unit 20 may be performed after the protection unit 20 is removed from the operation unit 30.
Next, the protection unit 20 to which the collection unit 10 is attached is removed from the operation unit 30. The protection unit 20 to which the collection unit 10 is attached is housed in a storage bag (not shown), and after the storage bag is sealed, a seal is attached to the outlet of the storage bag to ensure that it is not opened until the appraisal of fine objects. Taking a verification measure such as the signature of the witness in the entry box formed on the storage bag, the storage bag is handed over to the inspector. The inspector takes out the protection unit 20 to which the sampling unit 10 is attached from the storage bag, and further removes the sampling unit 10 from the protection unit 20 and performs microscopic observation using the sampling unit 10 as a subject, or from the sampling unit 10 Remove the gel sheet and perform DNA analysis.

The present invention is not limited to the above-described embodiment, and can be implemented with various modifications.
(1) The attachment means provided between the collection unit and the protection unit is not limited to the configuration of the above embodiment as long as the collection unit can be detachably attached to the protection unit, and various configurations can be adopted. it can. For example, one or two or more mounting protrusions are formed on both sides of the flat plate of the sampling part, a mounting hole to be fitted with the mounting protrusion is formed inside the upper edge of the protective part, and the sampling part is fitted from above can do. Further, the attachment means provided between the protection unit and the operation unit is not limited to the configuration of the above embodiment as long as the protection unit can be detachably attached to the operation unit, and various configurations can be adopted. For example, one or more mounting protrusions are formed on both sides of the protective part, a mounting hole for fitting with the mounting protrusion is formed inside the mounting base of the operation part, and the protective part is fitted from above. Can do.
(2) The shape of the flat plate of the sampling part is not limited to the substantially rectangular shape of the above embodiment, and can be various shapes such as a substantially square shape. For example, when the attachment means is configured in (1) above, the flat plate may be substantially trapezoidal. Moreover, the shape of the box-shaped body of the protection unit in plan view is not limited to the substantially square shape of the above-described embodiment, and may be various shapes such as a substantially trapezoidal shape. For example, when the attachment means is configured in the above (1) and the flat plate is substantially trapezoidal, the box-shaped body may be substantially trapezoidal.
(3) The box-shaped body of the protection portion is not limited to the configuration in which the upper portion and the front portion are opened as in the above-described embodiment, and may be configured to have only the upper portion opened. For example, when the attachment means is configured in the above (1), the front portion of the box-shaped body may not be opened.
(4) The operation unit is not limited to the above-mentioned shape as long as it has a shape that can be gripped and sampled easily and quickly, and may be formed in various shapes such as a prismatic body and a cylindrical body, for example. Can do.
(5) The collecting unit may be configured to attach the gel sheet directly to the flat plate without forming a stepped portion.

  EXAMPLES Next, although an Example is given and this invention is demonstrated in detail, this invention is not limited to a following example.

  In the case of a molester crime, since the scraps of the victim's clothing adhere to the suspect's hand, the scraps of the victim's clothing are collected as fines, and the scrap is collected from the suspect's hand. It may be possible to resolve molester crimes by judging whether they are the same. Therefore, each of the cotton fiber scraps and the polyester fiber scraps are touched by hand, and the fiber scraps adhering to the hand are separated with the above-mentioned fine sampling tool 100 (the following example) and a commercially available adhesive tape (the following comparative example). Attempts were made to test whether or not fines could be collected to the extent that they could be identified by both parties. The test results are shown in Tables 1 and 2.

  As shown in Table 1, in the case of cotton fiber litter, in the example, even when contacted for 1 second, the fiber litter attached to the hand could be collected to such an extent that it could be identified, but in the comparative example, there was no contact for 30 seconds or more. It was not able to be collected to the extent that it can be appraised. Therefore, the fine object collecting tool 100 according to the embodiment can verify a crime regardless of the length of time for the suspect's perverting action. Moreover, as shown in Table 2, in the case of the fiber waste of the polyester fiber, it was possible to collect the fiber waste adhering to the hand even in contact for 1 second in the example, but in the comparative example even in contact for 60 seconds. It was not able to be collected to the extent that it can be appraised. Therefore, the fine object collecting tool 100 of the embodiment can prove a crime regardless of the type of material of the victim's clothes. In addition, from the result that fines can be sufficiently collected even from a sampled portion having an uneven surface such as a hand, the sample collection tool 100 is excellent in adhesiveness and followability of the sampled portion of the sampled portion to the uneven surface, It was found to be extremely useful for fine article identification.

DESCRIPTION OF SYMBOLS 10 Sampling part 13 Gel sheet 14 Protective sheet 15 Flat plate 20 Protective part 21 Mounting groove 23 Mounting step part 25 Box-shaped body 30 Operation part 31 Mounting base 34 Mounting groove 36 Ellipsoidal columnar body 100 Fine object collection tool

Claims (6)

  A tool for collecting fine materials and DNA type identification materials that are present at crime scenes, etc., and a gel sheet of adhesive resin that covers a protective sheet is detachably attached to one surface A mounting unit provided between the sampling unit and the protection unit, the sampling unit including a flat plate, a protection unit including at least a box-shaped body having an open top, and an operation unit capable of gripping. The collection portion can be detachably attached to the protection portion, and when the collection portion is attached to the protection portion with the surface side to which the gel sheet is attached facing down, the protection portion has an inner bottom surface and the It is formed so that a gap is formed between the upper surface of the gel sheet, and the protective portion can be detachably attached to the operation portion by an attachment means provided between the protection portion and the operation portion. The fine part is characterized in that the protection part to which the collection part is attached is attached to the operation part, and the operation part is gripped and operated, and the fine part at the collection point is adhered to the upper surface of the gel sheet. Collection tool.   The attachment means, the protection part, and the operation provided between the collection part and the protection part so that the attachment / detachment direction of the collection part with respect to the protection part and the attachment / detachment direction of the protection part with respect to the operation part are orthogonal to each other The fine sampling tool according to claim 1, wherein the attachment means provided between the first and second portions is provided.   The fine material collecting tool according to claim 1 or 2, wherein the gel sheet is a polyurethane gel sheet having a rubber hardness of 30 or less.   The fine object collecting tool according to any one of claims 1 to 3, wherein the minute object is DNA type identification data or fiber waste.   A method for collecting and identifying a fine object using the fine object collecting tool according to any one of claims 1 to 3, wherein (1) a process of collecting the fine object on an upper surface of the gel sheet of the collecting unit And (2) a process of removing the sampling part from which the fine sample has been collected with the protection part attached to the operation part or removing the protection part from the operation part, and removing the protection part from the protection part. And (3) a process of attaching the collection part to the protection part with the surface side to which the gel sheet is attached facing down, and (4) attaching the protection part to the operation part in the process of (2). When removing the sampling part from which the fines have been collected while being attached from the protection part, the process of removing the protection part to which the sampling part is attached from the operation part, and (5) the attachment of the sampling part The protective part can be sealed A process of storing and storing in a container, and (6) a process of removing the sampling part from the protection part and observing the sampling part as a subject with a microscope, or removing the gel sheet from the sampling part and performing DNA analysis This is a method for collecting and appraising fine materials.   6. The method for collecting and appraising a fine material according to claim 5, wherein the fine material is DNA type identification data or fiber waste.

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