JP5222610B2 - A novel microorganism having a resolution of polyisoprene rubber and a method for decomposing a rubber composition - Google Patents
A novel microorganism having a resolution of polyisoprene rubber and a method for decomposing a rubber composition Download PDFInfo
- Publication number
- JP5222610B2 JP5222610B2 JP2008096521A JP2008096521A JP5222610B2 JP 5222610 B2 JP5222610 B2 JP 5222610B2 JP 2008096521 A JP2008096521 A JP 2008096521A JP 2008096521 A JP2008096521 A JP 2008096521A JP 5222610 B2 JP5222610 B2 JP 5222610B2
- Authority
- JP
- Japan
- Prior art keywords
- rubber
- polyisoprene
- strain
- nocardia
- resolution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 229920001971 elastomer Polymers 0.000 title claims description 79
- 239000005060 rubber Substances 0.000 title claims description 75
- 229920001195 polyisoprene Polymers 0.000 title claims description 35
- 239000000203 mixture Substances 0.000 title claims description 19
- 238000000034 method Methods 0.000 title claims description 17
- 244000005700 microbiome Species 0.000 title description 23
- 241001421890 Nocardia takedensis Species 0.000 claims description 21
- 241000186361 Actinobacteria <class> Species 0.000 claims description 14
- 241001446247 uncultured actinomycete Species 0.000 description 10
- 239000003795 chemical substances by application Substances 0.000 description 8
- 238000000354 decomposition reaction Methods 0.000 description 6
- 108020004465 16S ribosomal RNA Proteins 0.000 description 5
- 241000187654 Nocardia Species 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 244000043261 Hevea brasiliensis Species 0.000 description 4
- RRHGJUQNOFWUDK-UHFFFAOYSA-N Isoprene Chemical compound CC(=C)C=C RRHGJUQNOFWUDK-UHFFFAOYSA-N 0.000 description 4
- 230000015556 catabolic process Effects 0.000 description 4
- 238000006731 degradation reaction Methods 0.000 description 4
- 239000000806 elastomer Substances 0.000 description 4
- 229920003052 natural elastomer Polymers 0.000 description 4
- 229920001194 natural rubber Polymers 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
- 239000000446 fuel Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 238000004073 vulcanization Methods 0.000 description 2
- 239000013585 weight reducing agent Substances 0.000 description 2
- 235000014692 zinc oxide Nutrition 0.000 description 2
- 239000011787 zinc oxide Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 239000000899 Gutta-Percha Substances 0.000 description 1
- 229920004940 NATSYN® Polymers 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 240000000342 Palaquium gutta Species 0.000 description 1
- 239000006087 Silane Coupling Agent Substances 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229920000588 gutta-percha Polymers 0.000 description 1
- 229920006173 natural rubber latex Polymers 0.000 description 1
- 102000042567 non-coding RNA Human genes 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 239000010734 process oil Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000010058 rubber compounding Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/62—Plastics recycling; Rubber recycling
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Separation, Recovery Or Treatment Of Waste Materials Containing Plastics (AREA)
- Biological Depolymerization Polymers (AREA)
Description
本発明は、ポリイソプレン系ゴムの分解能を有する新規微生物及び該微生物を用いたゴム組成物の分解方法に関する。 The present invention relates to a novel microorganism having a resolution of polyisoprene rubber and a method for decomposing a rubber composition using the microorganism.
ゴム製品は一般のプラスチック製品とともに再利用しにくい材料であり、特にセメント工場などを中心として燃料として再利用されている場合が多い。しかしながら、近年環境問題の高まりとともに、ゴム製品を燃料として燃やすのではなく、コンポストのように低温で分解する省エネルギー型の方法や分解した材料を再利用するマテリアルリサイクルの方法の開発が求められている。 Rubber products are difficult to reuse with general plastic products, and are often reused as fuel, especially in cement factories. However, in recent years, with the growing environmental problems, it is required to develop energy-saving methods that decompose at low temperatures, such as compost, and material recycling methods that reuse decomposed materials, instead of burning rubber products as fuel. .
ゴム製品を分解する方法として、微生物による分解処理が考えられる。微生物分解は低温での処理なので、エネルギー消費は最も少ない処理法といえる。また、ゴム製品を微生物分解した後、分解物を再利用することも考えられる。例えば、分解されたモノマーやオリゴマーを再利用することや、粉ゴムの表面を微生物分解し、再度未加硫ゴムに混練り加硫することにより、新たな製品に利用することも考えられる。 As a method for decomposing a rubber product, a decomposing process using microorganisms is considered. Microbial degradation is a low-temperature treatment, so energy consumption is the least treatment method. It is also conceivable to recycle the decomposition product after microbial decomposition of the rubber product. For example, it is conceivable to reuse the decomposed monomer or oligomer, or to microbially decompose the surface of the powdered rubber and knead it again with the unvulcanized rubber and vulcanize it for a new product.
ゴムの微生物分解については、従来様々な微生物がスクリーニングされ、検討されている。そのような微生物分解については、特開平９−１９４６２４号公報（特許文献１）、特開平１１−６０７９３号公報（特許文献２）等で報告されており、これらの特許文献には、ノカルディア属に属する微生物によるゴムの分解方法が記載されている。 Various microorganisms have been screened and studied for microbial degradation of rubber. Such microbial degradation has been reported in JP-A-9-194624 (Patent Document 1), JP-A-11-60793 (Patent Document 2), and the like. A method for decomposing rubber by microorganisms belonging to the above is described.
しかしながら、ゴムの微生物分解の実用化に当たっては、より高効率でゴムを分解することが可能な微生物が望まれている。 However, in the practical application of microbial degradation of rubber, a microorganism capable of decomposing rubber with higher efficiency is desired.
そこで、本発明の目的は、従来より高効率でゴムを分解することが可能な新規な微生物及び該微生物によるゴムの分解方法を提供することにある。 Therefore, an object of the present invention is to provide a novel microorganism capable of decomposing rubber with higher efficiency than before and a method for decomposing rubber by the microorganism.
本発明者は、上記目的を達成すべく、日本各地の多くの土壌から天然ゴム分解菌の探索を行った結果、高効率でポリイソプレン系ゴムを分解できる微生物が驚くべきことに、ノカルディア・タケデンシス（Nocardia Takedensis）に帰属する放線菌であることを見出し、本発明を完成させるに至った。 As a result of searching for natural rubber-degrading bacteria from many soils all over Japan to achieve the above object, the present inventor has surprisingly found that microorganisms capable of degrading polyisoprene rubber with high efficiency are Nocardia It discovered that it was an actinomycete which belongs to Takedensis (Nocardia Takedensis), and came to complete this invention.
すなわち、本発明の新規微生物は、ポリイソプレン系ゴムの分解能を有する、ノカルディア・タケデンシス（Nocardia Takedensis）に帰属する放線菌ＢＳ−ＧＳ１株（FERM P-21535）であることを特徴とする。 That is, the novel microorganism of the present invention is characterized by the actinomycete BS-GS1 strain ( FERM P-21535 ) belonging to Nocardia Takedensis having the resolution of polyisoprene rubber.
また、本発明の別の新規微生物は、ポリイソプレン系ゴムの分解能を有する、ノカルディア・タケデンシス（Nocardia Takedensis）に帰属する放線菌ＢＳ−ＧＳ２株（FERM P-21536）であることを特徴とする。 Another novel microorganism of the present invention is the actinomycete BS-GS2 strain ( FERM P-21536 ) belonging to Nocardia Takedensis, which has the resolution of polyisoprene rubber. .
また、本発明の別の新規微生物は、ポリイソプレン系ゴムの分解能を有する、ノカルディア・タケデンシス（Nocardia Takedensis）に帰属する放線菌ＢＳ−ＧＳ５株（FERM P-21537）であることを特徴とする。 Another novel microorganism of the present invention is the actinomycete BS-GS5 strain ( FERM P-21537 ) belonging to Nocardia Takedensis having the resolution of polyisoprene rubber. .
また、本発明の別の新規微生物は、ポリイソプレン系ゴムの分解能を有する、ノカルディア・タケデンシス（Nocardia Takedensis）に帰属する放線菌ＢＳ−ＧＳ６株（FERM P-21538）であることを特徴とする。 Another novel microorganism of the present invention is the actinomycete BS -GS6 strain ( FERM P-21538 ) belonging to Nocardia Takedensis, which has the resolution of polyisoprene rubber. .
また、本発明の別の新規微生物は、ポリイソプレン系ゴムの分解能を有する、ノカルディア・タケデンシス（Nocardia Takedensis）に帰属する放線菌ＢＳ−ＧＳ７株（FERM P-21539）であることを特徴とする。 Another novel microorganism of the present invention is the actinomycete BS-GS7 strain ( FERM P-21539 ) belonging to Nocardia Takedensis having the resolution of polyisoprene rubber. .
また、本発明のゴム組成物の分解方法は、上記微生物により、ポリイソプレン系ゴムを含有するゴム組成物を分解することを特徴とする。 The method for decomposing a rubber composition of the present invention is characterized by decomposing a rubber composition containing a polyisoprene-based rubber by the microorganism.
また、本発明のゴム組成物の好適例において、前記ゴム組成物中のポリイソプレン系ゴムの含有量が１０質量％以上であることを特徴とする。 In a preferred embodiment of the rubber composition of the present invention, the polyisoprene rubber content in the rubber composition is 10% by mass or more.
また、本発明のゴム組成物の他の好適例において、前記ポリイソプレン系ゴムがシスポリイソプレンゴムであることを特徴とする。 In another preferred embodiment of the rubber composition of the present invention, the polyisoprene rubber is cis polyisoprene rubber.
本発明によれば、従来より高効率でゴム製品を分解することができるという有利な効果を奏する。 According to the present invention, there is an advantageous effect that the rubber product can be decomposed with higher efficiency than before.
以下、本発明を詳細に説明する。本発明の新規微生物は、ポリイソプレン系ゴムの分解能を有する、ノカルディア・タケデンシス（Nocardia Takedensis）に帰属する放線菌ＢＳ−ＧＳ１株、ＢＳ−ＧＳ２株、ＢＳ−ＧＳ５株、ＢＳ−ＧＳ６株、ＢＳ−ＧＳ７株である。上記５種の菌株は、本発明者が日本各地の多くの土壌から天然ゴム分解菌を鋭意スクリーニングした結果、得られたものである。これらの菌株は、それぞれ独立行政法人 産業技術総合研究所 特許生物寄託センターに２００８年３月１２日に受託されている。詳細には、ＢＳ−ＧＳ１株は受託番号FERM P-21535として、ＢＳ−ＧＳ２株は受託番号FERM P-21536として、ＢＳ−ＧＳ５株は受託番号FERM P-21537、ＢＳ−ＧＳ６株は受託番号FERM P-21538として、ＢＳ−ＧＳ７株は受託番号FERM P-21539として受託されている。 Hereinafter, the present invention will be described in detail. The novel microorganisms of the present invention are actinomycetes BS-GS1 strain, BS-GS2 strain, BS-GS5 strain, BS-GS6 strain, BS-GS6 strain belonging to Nocardia Takedensis having the resolution of polyisoprene rubber. -GS7 strain. The above five strains were obtained as a result of the inventor's earnest screening for natural rubber-degrading bacteria from many soils throughout Japan. These strains were entrusted to the Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology on March 12, 2008, respectively. Specifically, the BS-GS1 strain has the accession number FERM P-21535 , the BS-GS2 strain has the accession number FERM P-21536 , the BS-GS5 strain has the accession number FERM P-21537 , and the BS- GS6 strain has the accession number FERM. as P-21538, BS-GS7 shares have been deposited as accession number FERM P-21539.
上記５種のノカルディア・タケデンシスに帰属する放線菌の菌株は、無機塩の液体培地中でゴムを資化しながら増殖する。無機塩としては、窒素、リン、カリウム、カルシウム、マグネシウムなどが挙げられる。 Strains of actinomycetes belonging to the above five types of Nocardia Takedensis grow while assimilating rubber in a liquid medium of inorganic salts. Inorganic salts include nitrogen, phosphorus, potassium, calcium, magnesium and the like.
ノカルディア属の放線菌は、天然ゴムなどのポリイソプレン系ゴムを分解することが知られているが、ノカルディア・タケデンシスに帰属する放線菌において、ポリイソプレン系ゴムを分解することができるものはこれまでに報告されていない。さらに、上記５種の本発明の菌株は、従来報告されているノカルディア属に属する放線菌よりもポリイソプレン系ゴムを分解する能力が高く、したがって、これら５種の菌株を用いることで、従来よりも高効率でポリイソプレン系ゴムを含有するゴム製品を分解することが可能である。 Actinomycetes of the genus Nocardia are known to degrade polyisoprene rubbers such as natural rubber, but the actinomycetes belonging to Nocardia Takedensis are those that can degrade polyisoprene rubber. It has not been reported so far. Further, the five strains of the present invention have a higher ability to degrade polyisoprene rubber than the conventionally reported actinomycetes belonging to the genus Nocardia, and therefore, by using these five strains, It is possible to decompose a rubber product containing a polyisoprene rubber with higher efficiency.
上記５種の菌株の同定は、近年主流となっている１６ＳｒＲＮＡの比較に基づいて行った。以下に方法を詳細に述べる。菌株をＹＭ寒天培地（Ｂｅｃｔｏｎ Ｄｉｃｋｉｎｓｏｎ ＮＪ，ＵＳＡ）に植菌し、３０℃で５日間培養した。その後、この菌体からＰｒｅｐＭａｎ Ｍｅｔｈｏｄ（Ａｐｐｌｉｅｄ Ｂｉｏｓｙｓｔｅｍｓ、ＣＡ，ＵＳＡ）により、ゲノムＤＮＡの抽出を行った。抽出したゲノムＤＮＡを鋳型として、ＰＣＲにより、１６Ｓ Ｒｉｂｏｓｏｍａｌ ＲＮＡ遺伝子（１６Ｓ ｒＤＮＡ）のうち、５末端側約５００ｂｐの領域を増幅した。その後、増幅した塩基配列をシーケンスし、検体の１６Ｓ ｒＤＮＡ部分塩基配列を得た。得られた１６ＳｒＤＮＡの塩基配列から検体の相同性検索を、細菌基準株データベース及び国際塩基配列データベースから、検索に用いるアルゴリズムとしてＢＬＡＳＴを用いて行った。結果を下記の表１及び２に示す。 The above five strains were identified based on the comparison of 16S rRNA, which has become the mainstream in recent years. The method is described in detail below. The strain was inoculated into YM agar medium (Becton Dickinson NJ, USA) and cultured at 30 ° C. for 5 days. Thereafter, genomic DNA was extracted from the cells by PrepMan Method (Applied Biosystems, CA, USA). The extracted genomic DNA was used as a template to amplify a region of about 500 bp on the 5-terminal side of the 16S Ribosomal RNA gene (16S rDNA) by PCR. Thereafter, the amplified base sequence was sequenced to obtain a 16S rDNA partial base sequence of the specimen. The homology search of the specimen from the obtained 16S rDNA base sequence was performed using BLAST as an algorithm used for the search from the bacterial reference strain database and the international base sequence database. The results are shown in Tables 1 and 2 below.
これらの結果から、上記５種の菌株は、ノカルディア・タケデンシスに帰属する菌株であると同定した。 From these results, the above five strains were identified as strains belonging to Nocardia Takedensis.
また、本発明のゴム組成物の分解方法は、上記ノカルディア・タケデンシスに帰属する菌株を一種又は複数組み合わせて用いることを特徴とする。本発明を適用する対象となるポリイソプレン系ゴムとしては、主鎖がシスポリイソプレン及びトランスポリイソプレンであるゴムまたはエラストマーを挙げることができる。本発明においてシスポリイソプレンであるゴムが最も好ましいが、イソプレンと他のモノマーとの共重合体を主鎖とするゴムまたはエラストマーにも本発明を適応することが可能である。具体的なシスポリイソプレンとしては、それに限定されるものではないが、天然ゴムやＮａｔｓｙｎなどの合成シスポリイソプレンが例示され、トランスポリイソプレンとしてはガッタパーチャなどの天然系ゴムが例示される。 The method for decomposing a rubber composition of the present invention is characterized by using one or a combination of strains belonging to the above Nocardia Takedensis. Examples of the polyisoprene rubber to which the present invention is applied include rubbers or elastomers whose main chain is cis polyisoprene and trans polyisoprene. In the present invention, rubber which is cis polyisoprene is most preferable, but the present invention can also be applied to rubber or elastomer having a copolymer of isoprene and another monomer as a main chain. Specific examples of cis polyisoprene include, but are not limited to, natural rubber and synthetic cis polyisoprene such as Natsyn, and trans polyisoprene includes natural rubber such as gutta percha.
ゴム組成物を構成するゴムエラストマー中のポリイソプレン系ゴムの含有量が１０質量％以上であれば本発明の方法の適応が可能である。ゴムエラストマー中のポリイソプレンは５０質量％以上が好ましく、さらに好ましくは８０質量％以上であるが、その範囲に限定されるものではない。 If the content of the polyisoprene rubber in the rubber elastomer constituting the rubber composition is 10% by mass or more, the method of the present invention can be applied. The polyisoprene in the rubber elastomer is preferably 50% by mass or more, and more preferably 80% by mass or more, but is not limited to this range.
本発明で分解の対象となるゴムは、イソプレン系ゴムであれば、ゴム工業で通常使用されているシランカップリング剤、硫黄、加硫剤、加硫促進剤、加硫促進助剤、酸化防止剤、オゾン劣化防止剤、老化防止剤、プロセス油、亜鉛華（ＺｎＯ）、ステアリン酸、過酸化物等の通常の配合剤が配合されたものでよい。ただし、ゴム配合剤の中に上記５種の菌株の増殖を阻害する配合剤が配合されている場合は、ゴムを溶剤抽出し、これを除去する必要がある。 If the rubber to be decomposed in the present invention is an isoprene-based rubber, a silane coupling agent, sulfur, vulcanizing agent, vulcanization accelerator, vulcanization accelerating agent, antioxidant that are usually used in the rubber industry Ordinary compounding agents such as an agent, an ozone deterioration preventing agent, an antiaging agent, a process oil, zinc white (ZnO), stearic acid, and peroxide may be blended. However, when the rubber compounding agent contains a compounding agent that inhibits the growth of the above five strains, it is necessary to solvent-extract the rubber and remove it.
ゴム組成物の分解反応の条件としては、通常のノカルディア属に属する放線菌の培養方法に準じた条件を採用することができる。例えば、培養培地として、本発明の微生物の基質であるゴム組成物のほか、窒素、リン、カリウム、カルシウム、マグネシウムなどの無機塩類を含むものが挙げられる。 As the conditions for the decomposition reaction of the rubber composition, conditions according to the normal cultivation method of actinomycetes belonging to the genus Nocardia can be employed. Examples of the culture medium include those containing inorganic salts such as nitrogen, phosphorus, potassium, calcium and magnesium in addition to the rubber composition which is a substrate of the microorganism of the present invention.
分解反応における温度は２８〜３２℃、好ましくは３０℃である。また、ｐＨは６〜８、好ましくは７である。 The temperature in the decomposition reaction is 28 to 32 ° C, preferably 30 ° C. Moreover, pH is 6-8, Preferably it is 7.
例えば、分解反応は以下のようにして行うことができる。まず、分解対象となるゴム組成物とともに本発明の微生物を前培養する。次に、分解対象のゴム組成物０．１〜１０ｇ／Ｌと前培養した微生物１０７〜１０９／１００ｍｌを反応容器に入れ、上述したような温度及びｐＨの条件で２０日以上培養する。このような分解反応によって、分解対象のゴム組成物を高効率で分解することができる。 For example, the decomposition reaction can be performed as follows. First, the microorganism of the present invention is pre-cultured with the rubber composition to be decomposed. Then, microorganisms 10 7 ~10 9 / 100ml preincubated with the rubber composition 0.1 to 10 g / L of decomposed placed in a reaction vessel, and cultured for more than 20 days under the conditions of temperature and pH as described above. By such a decomposition reaction, the rubber composition to be decomposed can be decomposed with high efficiency.
以下、実施例で本発明を具体的に説明するが、本発明はこれらの実施例により限定されるものではない。 EXAMPLES Hereinafter, although an Example demonstrates this invention concretely, this invention is not limited by these Examples.
（実施例１）
シリコーン栓付きのガラス容器に準備した下記表３の組成の培地に、アセトン抽出済みの天然ゴムラテックス手袋の短冊状に切ったゴム片５ｇ／Ｌを入れ、９０℃で、オートクレーブ中で滅菌処理した。これに同様の手袋ゴムを唯一の炭素源として前培養したノカルディア・タケデンシス（Nocardia Takedensis）に帰属する放線菌ＢＳ−ＧＳ１株の菌液（１０８／１００ｍｌ）を加え、４０日間、３０℃、５０ｒｐｍで振盪培養し、１２１℃でオートクレーブ中で滅菌し、手袋ゴム片を取り出した。取り出した手袋ゴム片をアセトンで数回洗浄後、純水で洗浄し、充分に乾燥したことを確認後、重量測定を行った。
Example 1
A rubber piece 5 g / L cut into a strip of natural rubber latex gloves already extracted with acetone was put into a medium having the composition shown in Table 3 below prepared in a glass container with a silicone stopper, and sterilized at 90 ° C. in an autoclave. . Bacterial liquid of actinomycetes BS-GS1 strain attributable to precultured Nocardia Takedenshisu (Nocardia Takedensis) Similar glove rubber as the sole carbon source (10 8 / 100ml) was added, 40 days, 30 ° C., The mixture was cultured with shaking at 50 rpm, sterilized in an autoclave at 121 ° C., and a glove rubber piece was taken out. The glove rubber piece taken out was washed several times with acetone, then with pure water, and after confirming that it was sufficiently dried, the weight was measured.
（実施例２）
実施例１と同様の実験を、ノカルディア・タケデンシス（Nocardia Takedensis）に帰属する放線菌ＢＳ−ＧＳ２株を用いて行った。
(Example 2)
An experiment similar to that of Example 1 was performed using the actinomycete BS-GS2 strain belonging to Nocardia Takedensis.
（実施例３）
実施例１と同様の実験を、ノカルディア・タケデンシス（Nocardia Takedensis）に帰属する放線菌ＢＳ−ＧＳ５株を用いて行った。
(Example 3)
The same experiment as in Example 1 was performed using the actinomycete BS-GS5 strain belonging to Nocardia Takedensis.
（実施例４）
実施例１と同様の実験を、ノカルディア・タケデンシス（Nocardia Takedensis）に帰属する放線菌ＢＳ−ＧＳ６株を用いて行った。
Example 4
The same experiment as in Example 1 was performed using the actinomycete BS-GS6 strain belonging to Nocardia Takedensis.
（実施例５）
実施例１と同様の実験を、ノカルディア・タケデンシス（Nocardia Takedensis）に帰属する放線菌ＢＳ−ＧＳ７株を用いて行った。
(Example 5)
An experiment similar to Example 1 was performed using the actinomycete BS-GS7 strain belonging to Nocardia Takedensis.
（比較例）
実施例１〜５と同様の実験を土壌から分離した放線菌を用いて行った。
(Comparative example)
Experiments similar to Examples 1 to 5 were performed using actinomycetes isolated from soil.
実施例１〜５及び比較例の結果を表４に示す。 Table 4 shows the results of Examples 1 to 5 and the comparative example.
表４のように、実施例１〜５では、本発明の微生物を用いた場合の重量減少が２０〜３０％であったが、比較例では、重量減少が０％であった。よって、本発明の微生物は天然ゴムに対して大きな分解性を持つことが確認された。 As shown in Table 4, in Examples 1 to 5, the weight reduction when using the microorganism of the present invention was 20 to 30%, but in the comparative example, the weight reduction was 0%. Therefore, it was confirmed that the microorganism of the present invention has a large degradability with respect to natural rubber.
本発明の新規微生物は、タイヤなどのゴム製品の廃棄及び再利用においてゴム製品を分解する手段として有用である。 The novel microorganism of the present invention is useful as a means for decomposing rubber products in the disposal and reuse of rubber products such as tires.
Claims (8)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2008096521A JP5222610B2 (en) | 2008-04-02 | 2008-04-02 | A novel microorganism having a resolution of polyisoprene rubber and a method for decomposing a rubber composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2008096521A JP5222610B2 (en) | 2008-04-02 | 2008-04-02 | A novel microorganism having a resolution of polyisoprene rubber and a method for decomposing a rubber composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2009247241A JP2009247241A (en) | 2009-10-29 |
| JP5222610B2 true JP5222610B2 (en) | 2013-06-26 |
Family
ID=41308527
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008096521A Expired - Fee Related JP5222610B2 (en) | 2008-04-02 | 2008-04-02 | A novel microorganism having a resolution of polyisoprene rubber and a method for decomposing a rubber composition |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP5222610B2 (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103421815B (en) * | 2013-09-06 | 2015-12-02 | 中国农业科学院作物科学研究所 | A wide new gene qGS5 of rice grain dland molecule marking method |
| WO2025033325A1 (en) | 2023-08-04 | 2025-02-13 | 株式会社ブリヂストン | Method for decomposing crosslinked rubber |
| WO2025033298A1 (en) | 2023-08-04 | 2025-02-13 | 株式会社ブリヂストン | Method for decomposing organic material |
| JPWO2025033152A1 (en) | 2023-08-04 | 2025-02-13 | ||
| WO2025033362A1 (en) | 2023-08-04 | 2025-02-13 | 株式会社ブリヂストン | Method for decomposing crosslinked rubber |
| JPWO2025033265A1 (en) | 2023-08-04 | 2025-02-13 | ||
| JPWO2025033361A1 (en) | 2023-08-04 | 2025-02-13 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0276575A (en) * | 1988-09-14 | 1990-03-15 | Agency Of Ind Science & Technol | Semi-continuous decomposition of rubber with microorganism |
| JP2006180794A (en) * | 2004-12-28 | 2006-07-13 | Bridgestone Corp | Novel microorganism BS-TA1 strain, method for microbial degradation of rubber |
| JP4603880B2 (en) * | 2004-12-28 | 2010-12-22 | 株式会社ブリヂストン | Novel microorganism BS-HA1 strain, method for microbial degradation of rubber |
-
2008
- 2008-04-02 JP JP2008096521A patent/JP5222610B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP2009247241A (en) | 2009-10-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5222610B2 (en) | A novel microorganism having a resolution of polyisoprene rubber and a method for decomposing a rubber composition | |
| Maroof et al. | Identification and characterization of low density polyethylene-degrading bacteria isolated from soils of waste disposal sites | |
| Nawong et al. | Rubber gloves biodegradation by a consortium, mixed culture and pure culture isolated from soil samples | |
| Kim et al. | Polymer film-based screening and isolation of polylactic acid (PLA)-degrading microorganisms | |
| Zafar et al. | Biodegradation of polyester polyurethane during commercial composting and analysis of associated fungal communities | |
| Abe et al. | Microbial degradation of poly (butylene succinate) by Fusarium solani in soil environments | |
| Tatangelo et al. | Biological devulcanization of ground natural rubber by Gordonia desulfuricans DSM 44462T strain | |
| KR20170021002A (en) | Microbial agent for decomposition of food waste | |
| US20100203601A1 (en) | Process for surface activation and/or devulcanization of sulfur-vulcanized rubber particles | |
| EP1454942B1 (en) | Method of treatment of powder rubber for making its surface uneven | |
| Agarwal et al. | A critical examination of advanced approaches in green chemistry: microbial bioremediation strategies for sustainable mitigation of plastic pollution | |
| SEVAKUMARAN et al. | Bio-degradation of polyhydroxyalkanoates (PHA) films in soil and lake environment | |
| Karimi-Avargani et al. | Accelerating degradation of natural rubber latex gloves by a consortium of microorganisms in an agricultural soil sample | |
| Lao et al. | Degradation of scrap tyre by Bacillus sp.–Kinetic aspects of major environmental parameters and identification of potential growth substrates | |
| CN116144508A (en) | A Cladosporium Degradable Polyurethane Plastic and Its Application | |
| Baculi et al. | Biodegradation of high density polyethylene by bacteria isolated from root nodules of Phaseolus vulgaris | |
| Mazuryk et al. | Pseudomonas alloputida and Pseudomonas taiwanensis in a process of degradation of polymer materials | |
| JP4603880B2 (en) | Novel microorganism BS-HA1 strain, method for microbial degradation of rubber | |
| Berekaa et al. | Degradation of natural rubber by Achromobacter sp. NRB and evaluation of culture conditions | |
| JP2006180794A (en) | Novel microorganism BS-TA1 strain, method for microbial degradation of rubber | |
| JP4652147B2 (en) | Novel microorganism WU-YS09 strain, method for microbial degradation of rubber | |
| JP7032756B2 (en) | Organic acid decomposition method, novel organic acid decomposition microorganism and compost production method | |
| JP4467424B2 (en) | Microbial treated rubber powder and rubber composition containing the rubber powder | |
| Huong et al. | Degradation of deproteinized natural rubber by Gordonia sp. isolated from enrichment consortia | |
| Ibrahim et al. | Degradation of natural and synthetic rubber by Gordonia alkanivorans strain E1 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20110329 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20130305 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20130311 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20160315 Year of fee payment: 3 |
|
| R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| LAPS | Cancellation because of no payment of annual fees |