JP5226568B2 - Citrus pollen germination inhibitor and citrus pollen germination inhibition method - Google Patents
Citrus pollen germination inhibitor and citrus pollen germination inhibition method Download PDFInfo
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- JP5226568B2 JP5226568B2 JP2009065927A JP2009065927A JP5226568B2 JP 5226568 B2 JP5226568 B2 JP 5226568B2 JP 2009065927 A JP2009065927 A JP 2009065927A JP 2009065927 A JP2009065927 A JP 2009065927A JP 5226568 B2 JP5226568 B2 JP 5226568B2
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- pollen
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- pollen germination
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- 230000007198 pollen germination Effects 0.000 title claims description 17
- 235000020971 citrus fruits Nutrition 0.000 title claims description 15
- 241000207199 Citrus Species 0.000 title claims description 13
- 238000000034 method Methods 0.000 title claims description 9
- 239000003112 inhibitor Substances 0.000 title claims description 6
- 230000005764 inhibitory process Effects 0.000 title description 3
- 230000035784 germination Effects 0.000 claims description 15
- BDAGIHXWWSANSR-UHFFFAOYSA-M Formate Chemical compound [O-]C=O BDAGIHXWWSANSR-UHFFFAOYSA-M 0.000 claims description 10
- 229940044170 formate Drugs 0.000 claims description 10
- CBOCVOKPQGJKKJ-UHFFFAOYSA-L Calcium formate Chemical group [Ca+2].[O-]C=O.[O-]C=O CBOCVOKPQGJKKJ-UHFFFAOYSA-L 0.000 claims description 7
- 229940044172 calcium formate Drugs 0.000 claims description 7
- 235000019255 calcium formate Nutrition 0.000 claims description 7
- 239000004281 calcium formate Substances 0.000 claims description 7
- 230000002401 inhibitory effect Effects 0.000 claims description 7
- 239000004280 Sodium formate Substances 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 5
- HLBBKKJFGFRGMU-UHFFFAOYSA-M sodium formate Chemical compound [Na+].[O-]C=O HLBBKKJFGFRGMU-UHFFFAOYSA-M 0.000 claims description 5
- 235000019254 sodium formate Nutrition 0.000 claims description 5
- 239000004480 active ingredient Substances 0.000 claims description 2
- 150000004675 formic acid derivatives Chemical class 0.000 claims 3
- 229920001817 Agar Polymers 0.000 description 4
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000002420 orchard Substances 0.000 description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 235000010216 calcium carbonate Nutrition 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Description
本発明は花粉の発芽を阻害する技術に関する。 The present invention relates to a technique for inhibiting pollen germination.
中晩生のカンキツは種子が混入する品種が多く、商品性を低下させる大きな要因となっている。また、種子が果実の商品性を低下させるのはカンキツに限ったことではない。
例えば花粉発生を抑制すれば種子の生成を抑制できると予測でき、特開平６−４８９０５号公報（特許文献１）には、Ｆ１種子を効率的に生産するために化学的不稔剤を使用する技術が開示されている。
Mid-late citrus varieties are mixed with seeds, which is a major factor in reducing the merchantability. Moreover, it is not limited to citrus that seeds reduce the commercial value of fruits.
For example, it can be predicted that the generation of seeds can be suppressed if the generation of pollen is suppressed, and in JP-A-6-48905 (Patent Document 1), a chemical sterilant is used to efficiently produce F1 seeds. Technology is disclosed.
しかしながら、特許文献１の技術は果樹園での利用に適するものではなく、果樹農家において簡単にカンキツの種子混入を抑制できる技術が求められていた。 However, the technique of Patent Document 1 is not suitable for use in an orchard, and a technique that can easily suppress the mixing of citrus seeds in an orchard farmer has been demanded.
本願の請求項１に係る発明は、水溶性のギ酸塩を有効成分とするカンキツの花粉の発芽阻害剤を要旨とする。
また請求項３に係る発明は、ギ酸塩の水溶液をカンキツの果樹の花に散布することを特徴とするカンキツの花粉の発芽阻害方法である。
The gist of the invention according to claim 1 of the present application is a citrus pollen germination inhibitor containing a water-soluble formate as an active ingredient.
The invention according to claim 3 is a method for inhibiting germination of citrus pollen, characterized in that an aqueous solution of formate is sprayed on the flowers of a citrus fruit tree.
請求項１、３の発明に使用するギ酸塩はギ酸カルシウム又はギ酸ナトリウムである。
また、水溶液とした場合の希釈率は、２００倍以下（ギ酸塩の質量濃度が０．５％以上）である。
The formate used in the inventions of claims 1 and 3 is calcium formate or sodium formate .
In addition, the dilution rate in the case of an aqueous solution is 200 times or less (the mass concentration of formate is 0.5% or more) .
［試験１］
‘はるか’（品種名）の開花直前の花蕾を採取し、開葯した花粉をｎ−ヘキサンで純花粉に精製した。ショ糖１０％、寒天１％にギ酸カルシウム、ギ酸ナトリウム、炭酸カルシウム、塩化カルシウムをそれぞれ１．０％、０．５％、０．２％になるように調製した培地に花粉を散布し、２５℃で１６時間静置後、花粉の発芽率を調査した。また、２４時間静置後、花粉管長を測定した。対照として、ショ糖、寒天のみの培地を供試した。
［試験２］
‘せとか’、‘天草’、‘晩白柚’、‘はるか’及び‘はるみ’の花粉を供試し、ショ糖１０％、寒天１％にギ酸カルシウムの濃度を変えて添加した培地上に花粉を散布し、２５℃で１６時間静置後、花粉の発芽率を調査した。
［試験３］
‘はるか’から採取した花粉を供試し、塩酸と水酸化ナトリウムを用いてｐＨを調整したショ糖１０％、寒天１％の培地に散布し、２５℃で１６時間静置後、花粉の発芽率を調査した。
［結果および考察］
試験１：花粉の発芽はギ酸カルシウムおよびギ酸ナトリウムを含む培地で強く抑制され、それぞれ１．０％および０．５％の濃度ではまったく発芽が認められなかった（表１）。０．２％では発芽はみられたものの発芽率は低く、花粉管の伸長も短かった。炭酸カルシウムは花粉発芽率、花粉管長とも無処理と同程度であり、影響は認められなかった。塩化カルシウムは高濃度で花粉の発芽や花粉管の伸長を阻害したが、その程度はギ酸を含む塩類よりも低かった。
[Test 1]
The flower buds of “Haruka” (variety name) immediately before flowering were collected, and the opened pollen was purified to pure pollen with n-hexane. Pollen is sprayed on a medium prepared with 1.0%, 0.5%, and 0.2% calcium formate, sodium formate, calcium carbonate, and calcium chloride on 10% sucrose and 1% agar, respectively. 25 After standing at ℃ for 16 hours, pollen germination rate was investigated. Moreover, pollen tube length was measured after leaving still for 24 hours. As a control, a medium containing only sucrose and agar was used.
[Test 2]
Try "Setoka", "Amakusa", "Yakuhaku", "Haruka" and "Harumi" pollen on a medium containing 10% sucrose and 1% agar with varying concentrations of calcium formate. After standing for 16 hours at 25 ° C., pollen germination rate was investigated.
[Test 3]
The pollen collected from 'Haruka' was tested, sprayed on a medium of 10% sucrose and 1% agar adjusted to pH using hydrochloric acid and sodium hydroxide, and allowed to stand at 25 ° C for 16 hours. investigated.
[Results and Discussion]
Test 1: Pollen germination was strongly suppressed in a medium containing calcium formate and sodium formate, and no germination was observed at concentrations of 1.0% and 0.5%, respectively (Table 1). At 0.2%, germination was observed, but the germination rate was low, and the pollen tube elongation was short. Calcium carbonate had the same pollen germination rate and pollen tube length as untreated, and no effect was observed. Calcium chloride inhibited pollen germination and pollen tube elongation at high concentrations, but to a lesser extent than salts containing formic acid.
以上のことから、ギ酸塩はカンキツの花粉の発芽抑制や花粉管の伸長阻害に高い効果があると考えられた。また、その濃度が２００倍以上ではほとんど花粉が発芽しないことが明らかとなった。さらにｐＨが５．０以上では花粉発芽率に差がみられないことから、ギ酸塩の発芽抑制効果はｐＨに由来するものではないと思われる。 From the above, it was considered that formate is highly effective in suppressing germination of citrus pollen and inhibiting the elongation of pollen tubes. Moreover, it became clear that the pollen hardly germinates when the concentration is 200 times or more. Furthermore, since there is no difference in the pollen germination rate when the pH is 5.0 or more, it is considered that the germination inhibitory effect of formate is not derived from pH.
試験１〜３により、請求項１、２に記載の発芽阻害剤には花粉の発芽を阻害する効能があることが確認できた。そして、請求項３、４に記載の発芽阻害方法を実施すれば、果樹の花粉の発芽を阻害することができる。これにより、カンキツ等の果樹（その花）に本発明の発芽阻害剤を散布すれば（すなわち発芽阻害方法を実施すれば）、種子の混入による商品性の低下を抑止できる。しかも、ギ酸塩の水溶液を果樹の花に散布するという簡単な施用作業で済むから、果樹園での利用は容易である。 From tests 1 to 3, it was confirmed that the germination inhibitor according to claims 1 and 2 has an effect of inhibiting pollen germination. And if the germination inhibition method of Claim 3 and 4 is implemented, the germination of the pollen of a fruit tree can be inhibited. Thereby, if the germination inhibitor of this invention is sprayed on fruit trees (the flower), such as a citrus (that is, if the germination inhibition method is implemented), the merchantability fall by mixing of a seed can be suppressed. In addition, it is easy to use in an orchard because a simple application operation of spraying an aqueous solution of formate onto the flowers of the fruit tree is sufficient.
Claims (2)
前記ギ酸塩がギ酸カルシウム又はギ酸ナトリウムであり、
前記ギ酸塩の質量濃度が０．５％以上であることを特徴とするカンキツの花粉の発芽阻害剤。 A germination inhibitor pollen of Citrus that the water-soluble formate salt as an active ingredient,
The formate salt is calcium formate or sodium formate;
A citrus pollen germination inhibitor, wherein the formate has a mass concentration of 0.5% or more.
前記ギ酸塩がギ酸カルシウム又はギ酸ナトリウムであり、
前記水溶液における前記ギ酸塩の質量濃度が０．５％以上であることを特徴とするカンキツの花粉の発芽阻害方法。 A method for inhibiting germination of citrus pollen, characterized in that an aqueous solution of formate is sprayed on a flower of a citrus fruit tree ,
The formate salt is calcium formate or sodium formate;
A method for inhibiting germination of citrus pollen, wherein the mass concentration of the formate in the aqueous solution is 0.5% or more.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009065927A JP5226568B2 (en) | 2009-03-18 | 2009-03-18 | Citrus pollen germination inhibitor and citrus pollen germination inhibition method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009065927A JP5226568B2 (en) | 2009-03-18 | 2009-03-18 | Citrus pollen germination inhibitor and citrus pollen germination inhibition method |
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| Publication Number | Publication Date |
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| JP2010215583A JP2010215583A (en) | 2010-09-30 |
| JP5226568B2 true JP5226568B2 (en) | 2013-07-03 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3093702B2 (en) * | 1996-12-19 | 2000-10-03 | 晃栄化学工業株式会社 | Flower remover and flowering method |
| JP2000198704A (en) * | 1999-01-06 | 2000-07-18 | Shiraishi Calcium Kaisha Ltd | Flower-thinning agent for apple tree |
| JP2001206805A (en) * | 2000-01-25 | 2001-07-31 | Koei Kagaku Kogyo Kk | Defloration agent and method of defloration |
| JP2001328910A (en) * | 2000-05-22 | 2001-11-27 | Shiraishi Calcium Kaisha Ltd | Orchard for fruit trees |
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