JP5343193B2 - Biological behavior control method and apparatus - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an apparatus for controlling organism behavior, capable of appropriately observing the organism behavior. <P>SOLUTION: This apparatus 10 for controlling the organism behavior is used when observing the organism behavior, and has a polarization irradiating mechanism 12 which produces a plurality of lights 14 and 16 different only in a polarization condition. The polarization irradiating mechanism 12 simultaneously irradiates the lights 14 and 16 in a polarization condition each corresponding to a plurality of different object parts 18a and 18b of an organism 18 which is to show behavior to the lights 14 and 16, and controls polarization characteristics in the behavior of the organism when observing the polarization characteristics in the behavior of the organism 18. <P>COPYRIGHT: (C)2009,JPO&amp;INPIT

Description

The present invention particularly relates to biological behavior caused by irradiation of a chiral light to equipment of the observation method Oyo benefactor. More particularly, relates to a technique for observing the biological behavior caused by irradiating an organism with special polarization such as circular polarization, for example, circularly polarized light and counterclockwise circularly polarized light clockwise plants such sheet Roinunazuna by irradiation, a method and apparatus for monitoring the growth of the plant.

  Light represented by sunlight is a fundamental energy source in the living world, including plant photosynthesis, and is represented by light in various forms, as represented by efficient production of vegetables using light-emitting diodes. Biological control has been put into practical use. In order to efficiently control such organisms, it is very important to understand the behavior of organisms with respect to light.

Conventionally For this, for example, different ultraviolet wavelength ranges, i.e. UVA (320 ~ 400nm), UVB (280~320 nm), was irradiated UVC the (~ 280 nm) in cultured cells, the effect on cell cultures of these ultraviolet (For example, Non-Patent Document 1) .
Toyo Iwata, National Institute of Advanced Industrial Science and Technology, “Examination of the action of ultraviolet rays on cultured cells”

However, in the long et al accordance come technology, only the wavelength characteristics may be described convincingly is observed of not obtained behavior, the behavior of the organism, was not intended to necessarily say is to be fully grasped. Thus, in the field of this, although the development of a technique that can examine the behavior of the organism exactly has been strongly desired, conventionally, the behavior indicated by the organism to light much is still unclear, this There was no suitable technology that could be solved. The present invention has been made in view of the problems of the sub coming technology, and its object is to provide a biological behavior observation method and apparatus capable of appropriately observing the behavior of the raw material.

  As a result of intensive studies on the above problems by the present inventors, it has been discovered that in order to efficiently control the behavior of a living organism, it is extremely important to grasp the polarization characteristics in the behavior of the living organism. It came to complete.

That is, in general, light such as sunlight is not in a special polarization state, and provides a benefit to the living world in a form in which light in various directions is combined. On the other hand, most of the components constituting organisms are optically active substances, and these have optical anisotropy such as optical rotation and circular dichroism with respect to circularly polarized light. If the behavior of a living organism can be controlled by light used for evaluation of optical activity such as circularly polarized light, it becomes possible to control the behavior of the organism by light with higher efficiency.
However, since living organisms grow under unpolarized natural light, there has been no concept of polarization characteristics in the past. For this reason, conventionally, there was no concept of controlling the behavior of organisms by special light such as circularly polarized light. However, the present inventors have proposed how organisms such as plants and insects can react to optically active light. It has been found that it is very important to clarify whether the behavior is shown. Based on such knowledge, a problem-solving means of irradiating a biological sample with chiral light, for example, left and right circularly polarized light having the same intensity and wavelength, and observing the polarization characteristics during growth of the biological sample has been adopted.

Biological behavior observation method
To achieve the above Symbol purpose, engaging Ru biological behavior monitoring method of the present invention, only the polarization state to two individuals of the same kind of organism to separate two different polarization simultaneously irradiated, of the two individual It is a method used for observation of biological behavior that varies depending on the polarization characteristics . The “individual” may be a single individual or a plurality of individuals of the same species. The same applies to the following description.

Biological Behavior observation apparatus In order to achieve the above Symbol purpose, engaging Ru biological behavior observation apparatus in the present invention, Ru Tei equipped with a polarizing illumination mechanism only the polarization state is irradiated simultaneously produce two different polarization . Polarized irradiation mechanism of that, only the polarization state to two individuals of the same species of an organism is irradiated different separated two polarization simultaneously, the apparatus used for observing different behaviors by the polarization properties of the two individual It is .

To two individuals of the same species of an organism referred to herein, and only the polarization state is irradiated different separated two polarized light at the same time, to use meaning the two individuals of the same species of organism, the same This refers to the case of simultaneously irradiating two individuals with different polarization states only in the surrounding environment .

In the present invention, the polarized light irradiation mechanism may be a plurality of lights that differ only in the polarization state, such as left and right circularly polarized light, first linearly polarized light and second linearly polarized light orthogonal to the first linearly polarized light, or left and right elliptically polarized light. It is preferable to generate them simultaneously.
Here, the left and right elliptical polarizations are those in which the major axis of the left elliptical polarization and the major axis of the right elliptical polarization are orthogonal and the ellipticity of the left elliptical polarization and the ellipticity of the right elliptical polarization are equal. Say.

Or In the present invention was, polarized light irradiation mechanism, and one light source, a wavelength selective filter, it is preferable and a polarized light generating means. Here in the previous Symbol light source emits light. Also wavelength selective filter, the light from the light source and monochromatic light of a desired wavelength. Polarized light generation means, the monochromatic light from the wavelength selective filter, only the polarization state to generate two different polarization.

<Wollaston prism>
In the present invention, it is preferable that the polarized light generating means includes a Wollaston prism.
Here, the Wollaston prism splits monochromatic light from the wavelength selection filter into first linearly polarized light and second linearly polarized light simultaneously.

<Wave plate>
In the present invention, it is preferable that the polarized light generating means further includes a wave plate. Its wavelength plate simultaneously a first linear polarized light and the second linearly polarized light from the front Symbol Wollaston prism, right and left circular polarization of the pair, the pair of the second linearly polarized light orthogonal to the first linear polarization and said straight line polarized light, or convert the left and right elliptical polarization pairs.

<Wave plate rotation mechanism>
In the present invention, it is preferable to provide a wave plate rotating mechanism.
Here, the wave plate rotating mechanism holds the wave plate rotatably around the optical axis in a plane perpendicular to the optical axis of the wave plate.

<Irradiation lens>
In the present invention, polarized light irradiation mechanism, it is preferable to comprise a a morphism light of the further lens and illumination lens rotation mechanism. Here irradiation morphism lens polarization pairs that will be generated by the polarized irradiation mechanism, the two individuals of the same species of organism, is irradiated to each. The irradiation lens rotating mechanism, as the light intensity in the two individuals from the lens morphism irradiation is equal, the lens morphism irradiation its optical axis is held as it is adjustable.

<Partition Ri plate>
In the present invention, it is preferable to comprise a specification Setsu Ri plate between the bottom surface of the polarized light irradiation mechanism and the sample container. Here, plate Ri Setsu specification will partition the polarization pairs emitted from the polarized irradiation mechanism into two individuals of the same species of organism.

According to the biological behavior observation method and apparatus therefor according to the present invention, since the behavior of different organisms is observed according to the polarization characteristics of two individuals of the same species, the behavior of the organism due to polarization is efficiently observed . be able to.

In the present invention, the common polarized irradiation mechanism in the polarization state, the light source, Wollaston prism, by providing a wave plate or the irradiation lens can be the same Ri by the observation conditions other than the polarization state since, different biological behavior by the polarization properties of the two individuals of the same species of organisms but can be observed more appropriately. In the present invention, by providing a partition Ri plate from the polarized irradiation mechanism separates different polarization pairs of polarization states of the two individuals of the same species of organism, different organisms behave the polarization characteristics of the two individuals of the same species of organism a, it can be observed more appropriately.

Hereinafter, a preferred embodiment of the present invention will be described with reference to the drawings. FIG. 1 shows a schematic configuration of a biological behavior observation apparatus 10 according to an embodiment of the present invention. 1A is a longitudinal sectional view of the biological behavior observation apparatus 10 according to the present embodiment , that is, a longitudinal sectional view taken along a line AA in FIG . 1B . Then, FIG. 1 (B) is a cross-sectional view of the biological behavior observation apparatus 10 according to the cross section of the line B-B in FIG. 1 (A). A biological behavior observation apparatus 10 shown in FIG. 1 includes a polarized light irradiation mechanism 12 and performs the biological behavior observation method of the present invention.

Here, the polarized light irradiation mechanism 12 simultaneously generates the left and right circularly polarized lights 14 and 16 having different polarization states only. The polarized light irradiation mechanism 12 for the two individuals of such chiral behavior to light will indicate (light response reaction) of the same species organisms, left right circularly polarized light 14 and 16 respectively Irradiating at the same time.

Therefore, in this embodiment, the polarized light irradiation mechanism 12 is common to the left and right circularly polarized light, a light source power supply 19, a light source 20, a condenser lens 22, a wavelength selection filter 24, a Wollaston prism 26, It includes a quarter-wave plate 2 8, an irradiation lens 30.

Here, the light source 20 emits light 32 by the power from the light source power source 19.
The condensing lens 22 condenses the light 32 from the light source 20.
Wavelength selection filter 24, a monochromatic light 34 of constant wavelength where the light 32 from the condenser lens 22.
The Wollaston prism 26 splits the monochromatic light 34 from the wavelength selection filter 24 into first linearly polarized light and second linearly polarized light. Quarter-wave plate 28, a first linear polarized light from the Wollaston prism 26, and a second linearly polarized light, the left circularly polarized light 14, at the same time to generate the right circularly polarized light 16. The irradiation lens 30 irradiates the left and right circularly polarized lights 14 and 16 simultaneously to two individuals of the same species of organisms separated by the partition plate 42 in the sample container .

The biological behavior observation apparatus 10 according to the present embodiment is configured as outlined above, and the operation thereof will be described below. That is, in the present embodiment, the polarized light irradiation mechanism 12 determines the chiral light, that is, the left and right polarized light 14 and 16 in which the observation conditions other than the polarization state are kept the same simultaneously to two individuals of the same species. Irradiation only for For this reason, according to the present embodiment, different biological behaviors can be accurately examined according to the polarization characteristics of the two individuals .

Next, the operation of this embodiment will be described more specifically.
First, the biological behavior observation apparatus 10 according to the present embodiment separates two individuals of the same species of living organisms in two divided regions of the sample container . Thus, in this embodiment, the observation conditions other than the polarization state can be made the same by using two individuals of the same species of organism as the biological sample. As a result, the biological behavior that differs depending on the polarization characteristics of the two individuals can be examined more accurately. For example, it is possible to accurately investigate the difference in energy efficiency between the two individuals when the counterclockwise circularly polarized light 14 is irradiated and when the clockwise polarized light 16 is irradiated.

In this embodiment, two individuals of the same species are irradiated with left and right circularly polarized light 14 and 16 having the same observation conditions other than the polarization state. That is, in the present embodiment, the light 32 from the one light source 20 emitted by the power supply from one of the light source power supply 19 is focused by the focusing lens 22 and enters the wavelength selection filter 24. Light 32 from the condenser lens 22 and enters the Wollaston prism 26 is a monochromatic light 34 at a constant wavelength by the wavelength selective filter 24. Monochromatic light 34 from the wavelength selection filter 24, a Wollaston prism 26, enters the first linear polarized light and the second linearly polarized light and is divided into that in the quarter-wave plate 28. The first linearly polarized light and the second linearly polarized light from the Wollaston prism 26 are converted into left and right circularly polarized light 14 and 16 by the quarter wavelength plate 28 and enter the irradiation lens 30. The left and right circularly polarized light 14 and 16 from the Wollaston prism are irradiated to two individuals of the same species by the irradiation lens 30.

In the present embodiment, irradiation of two organisms of the same species with the left and right circularly polarized lights 14 and 16 is continuously performed for a predetermined time, and the behavior of the organisms of two individuals that differ depending on the polarization characteristics is observed. At this time, in this embodiment, it is possible to irradiate two separated individuals of the same species with left and right circularly polarized light 14 and 16 having the same conditions other than the polarization state. That is, in this embodiment, by operating one of the light source 20 by the power from one of the light source power supply 19. Then, in this embodiment, from one of the light 32 from the one of the light sources 20, the left and right circularly polarized light 14 and 16 are generated simultaneously.
As a result, in the present embodiment, the left and right circularly polarized lights 14 and 16 are compared with those provided with a light source power source and a light source, respectively, and the difference in observation conditions caused by the light source power source and the light source is surely avoided. the observation conditions other than the state, between the left and right circularly polarized light 14 and 16 can be the same more accurately.

In the present embodiment, instead of generating alternately two circularly polarized light 14 and 16, the Wollaston prism 26 and the quarter-wave plate 28, the left and right two circularly polarized light 14 and 16 at the same time generating doing. In the present embodiment, the two individual left and right circularly polarized lights 14 and 16 are not alternately irradiated to two separated individuals of the same species , but the Wollaston prism 26 and the quarter wavelength plate 28 are used. and irradiating the two circularly polarized light 14 and 16 that will be produced at the same time at the same time by.

As a result, a difference in observation conditions due to the time difference between when the left circularly polarized light 14 is irradiated and when the right circularly polarized light 16 is irradiated is surely avoided, and the observation conditions other than the polarization state are changed to the left and right circularly polarized light 14, It is possible to make the same between 16 more accurately. Therefore, since the two individuals of the same species can be irradiated with the left and right circularly polarized light 14 and 16 having the same intensity and wavelength, it is possible to appropriately observe the difference in the growth state due to the polarization characteristics of the two individuals. it can.

According to this embodiment, under illumination polarization states two different polarization, and other conditions are most different without differences in biological behavior of the polarization characteristics of the two individuals of the same species of organism By accurately grasping it, it can be used for efficient observation of the biological behavior of two individuals of the same species . This than was observed such wavelength characteristics in an organism elevation movement as in the prior art are those obtained never, by observation of the biological behavior of the polarization characteristics of the two individuals of the same species of organism present invention This is the first effect.

Improved accuracy
In the present embodiment, in order to more accurately observe biological behavior of two individuals of the same species, it is very important to make observation conditions other than the polarization state more exactly the same. Therefore, in this embodiment, the sample dish below the partition Ri plate, it is preferable to use a rotating mechanism.

<Sample Petri dish>
That is, in this embodiment, it is also very important to the same exact two individual peripheral environment of the same type of organism. Therefore, in this embodiment, it is also very important to use a sample dish 40 to put the two individuals of the same species of organism, such as are shown in FIG. 2 (A) is a longitudinal sectional view of such a sample dish 40 and the partition plate 42, FIG. 2 (B) is a plan view of such the specimen dish 40 and the partition plate 42. That is , FIG . 2A is a longitudinal sectional view taken along the line AA ′ in FIG .
In the present embodiment, as a container to put the two individuals of the same species of organism, and by using a sample dish 40, to put each of the two individuals in the two regions partitioned by the partition plate 42 of the sample dish 40 Yes. Then it is performed the irradiation of the left-handed circularly polarized light 14 into one of two individuals, taking into specimen dish 40, an irradiation of the right circularly polarized light 16 to the other at the same time. As described above, in this embodiment, by performing the two individuals of the same species of organism observation different organisms behave the polarization characteristics put one on two regions of the sample dish 40, the circumferential sides of each individual The environment can be made more exact the same. Therefore, in this embodiment, the biological behavior due to the polarization characteristics of the same species of organism can be examined more accurately.

<Partition Ri plate>
Further, in this embodiment, in order to more accurately observe the biological behavior based on the polarization characteristics of two individuals of the same species, it is very important to irradiate the two individuals with two polarized lights having different polarization states. is important. Therefore, in the present embodiment is provided with the partition Ri plate 42 as is shown in Fig 2. Partition Ri plate 42 from directly below the polarized irradiation mechanism 12 in FIG. 1 (A) is provided between the to the bottom surface of the sample dish 40 is attached to the support plate 44, the polarized irradiation mechanism 12 of the same type of organism The left circularly polarized light 14 and the right circularly polarized light 16 are divided into two individuals . In the present embodiment, by contacting a lower end of the partition Ri plate 42 to the bottom surface of the sample dish 40 without gaps, ensure that the left-handed circularly polarized light 14 to be irradiated to one individual is irradiated to the other individual blocked, and right circularly polarized light 16 to be irradiated to the other individual can be reliably blocked from being radiated to one individual.
As a result, in the present embodiment, one of the two individuals can be reliably irradiated with only the left circularly polarized light 14 and the other individual can be reliably irradiated with only the right circularly polarized light 16. Therefore, in this embodiment, since the influence of polarization other than predetermined can be surely eliminated, the biological behavior due to the polarization characteristics of two individuals of the same species can be examined more accurately.

Incidentally, in FIG. 2 (A), the on the bottom surface of the sample dish 40, the distance L is a predetermined distance between the center axis X _B of the central axis X _A and right circularly polarized light 16 of the left-handed circularly polarized light 14 (e.g., L = 50 mm) to have a left-handed circularly polarized light 14 and the right circularly polarized light 16 is separation. In FIG. 2 (B), respectively left and right circularly polarized light 14 and 16, the beam size phi _A was determined based on the two individual size on the bottom surface of the sample dish 40, phi _B (e.g., φ _A = φ _B = 40 mm).

In the present embodiment, the partition Ri plate 42, making the unirradiated state of blocking the left and right circularly polarized light 14 and 16 to two individuals of the same species of organism, can be performed to observe the irradiation zero. Then, by evaluating on the basis of the state of zero irradiation, evaluation of one individual irradiated with the left circularly polarized light 14 or evaluation of the other solid irradiated with the right circularly polarized light 16 can be performed. A more accurate evaluation can be performed as compared with an evaluation that is not provided.

<Wave plate rotation mechanism>
In the observation of the biological behavior of two individuals of the same species, there is also a desire to change only the polarization state without moving the two individuals . Therefore, in the present invention, it is preferable to provide a wave plate rotating mechanism.
Waveplate rotation mechanism of the present invention, around the optical axis in a plane perpendicular to the optical axis of the wavelength plate 28, rotatably holding the wave plate 28.

Therefore, in this embodiment, as a wavelength plate rotation mechanism includes a wave plate holder 54, as is shown in Fig 3. 3 (A) is a side view of such a wavelength plate 28 and the support plate 50, FIG. 3 (B) is a plan view of such the wave plate 28 and the support plate 50. 3, 1/4-wavelength plate 2 8, with respect to the support plate 50 to the optical components of the polarized light irradiation mechanism is set, rotatably about the optical axis 52 in a horizontal plane perpendicular to the optical axis 52 Is provided. For this purpose, in this embodiment, the wave plate holder 54 includes a lower holder 54 a and an upper holder 54 b, and an operation knob 56. Here, the lower holder 54a and the upper holder 54b is placed on the support plate 50 in the horizontal direction, that have quarter-wave plate 28 on the lower holder 54a is rotatably held around the optical axis 52. The operation knob 56 is provided on the side wall portion of the 1/4-wavelength plate 28, Ru rotate the 1/4-wave plate 28.

When it is desired to change only the polarization state without moving two individuals of the same species , that is, unlike FIG. 2 , one individual is irradiated with the right circularly polarized light 16 and the other individual is subjected to the left circularly polarized light. 14 when desired to be irradiated with the wave long plate 28 by rotation of the operating knob 56 is rotated 90 degrees about the optical axis 52 on the lower holder 54a. Then, in the present embodiment, without moving the two individuals can be performed only changes in the polarization state of polarized light irradiation.

In this way, the quarter wavelength plate 28 is provided with a wavelength plate rotating mechanism such as a wavelength plate holder 54 around its optical axis, and by rotating the quarter wavelength plate 28 90 degrees, Has a function to replace. Further, by setting the 1/4-wave plate 28 to the 45 degree orientation, the polarization state can be obtained two linearly polarized light orthogonal as irradiation light does not change. If the quarter-wave plate 28 is set in the middle axis direction between these, the irradiation light to two individuals of the same species is generally left and right whose major axis directions are orthogonal and the absolute value of the ellipticity is equal. The elliptically polarized light can be obtained. Therefore, in this embodiment, even if the polarization state is changed by the wave plate holder 54 that is the wave plate rotating mechanism as described above, the observation conditions other than the polarization state are more than two orthogonally polarized polarized light. Since it is kept exactly the same, it is possible to more accurately investigate the biological behavior due to the polarization characteristics of two individuals of the same species .

< Irradiation lens rotation mechanism>
In this embodiment, in order to accurately observe the biological behavior due to the polarization characteristics of two individuals of the same species, it is also very important to make the irradiation intensity of light to each target part exactly the same. . Therefore, in the present invention, it is preferable to provide an irradiation lens rotation mechanism. Irradiation lens rotation mechanism of the present invention, as the light intensity in the two individuals of the same species of organism are equal, the illumination lens, the optical axis is held as it is adjustable.

Therefore, in this embodiment, as an irradiation lens rotating mechanism, Ru Tei includes an irradiation lens holder 60 for holding the illumination lens 30, as is shown in Fig 4 (A). In FIG. 4A , the irradiation lens holder 60 makes the irradiation lens 30 rotatable with respect to the support plate 44 around the horizontal axis 62 in the direction of the arrow in the figure. As a result, in the present embodiment, the irradiation lens holder 60 can reliably adjust the optical axis of the irradiation lens 30 so that the irradiation light intensity to the two individuals of the same species is equal to each other. Therefore, it is possible to more accurately match the irradiation intensities of two polarized lights having different polarization states to the two individuals .

In the present embodiment, the adjustment of the irradiation light intensity by irradiating the lens 30, as are shown in FIG. 4 (B), the respective power meter 64a to the irradiation position of the left and right circularly polarized light 14 and 16, sets the 64b It is also very preferable to carry out. That is, in the present embodiment, the rotation angle of the optical axis of the irradiation lens 30 in the arrow direction in the drawing is adjusted by the power meters 64a and 64b so that the power meter intensities of the circularly polarized light 14 and 16 are the same. ing. As a result, in the present embodiment, the irradiation intensity of the circularly polarized light 14 and 16, compared to the case of adjusting by trial and error, since more precise can be the same, the biological behavior dynamic by polarization characteristics, and more precisely Can be examined.

Modification <Type of polarization>
Incidentally, in the configuration, with reference to FIG. 1 (A), the polarized irradiation mechanism 12, i.e. the quarter-wave plate 28, two of the linearly polarized light the left and right circularly polarized light pair from Wollaston prism 26 However, the present invention is not limited to this example, and any polarization pair may be generated as long as the polarization state is different. For example, the pair of the second linearly polarized light orthogonal to the first linear polarization and said straight line polarized light, more half-wave plates, orthogonal to the first linear polarization and said straight line polarized light separated by the Wollaston prism 26 It is obtained by converting into a pair with the second linearly polarized light. Further, a pair of left and right of the elliptically polarized light, the other wavelengths plate, giving a phase difference other than the phase difference circularly polarized light and the linearly polarized light is obtained in the linear polarization, two linearly polarized light from the Wollaston prism 26 Is converted into left and right elliptically polarized light.

Hereinafter, an embodiment of the present invention will be described. In this embodiment, around the left, and the irradiation area of the right-handed circularly polarized light, the Arabidopsis thaliana seeds were allowed to stand as a biological sample, and observing the behavior of each case in the biological behavior observation apparatus 10.
That is, in this embodiment, the straight line polarizing light having a wavelength of 670nm orthogonal by Wollaston prism 26, through quarter wave plate 45 degree orientation bisecting the polarization direction, and adjacent pairs of right and left circularly polarized light Generated . These left and right circularly polarized light 14 and 16, so as to be able to irradiate the two regions of the sample dish 40, to prepare a raw material behavior observation apparatus 10. Then, Arabidopsis seeds were placed on the counterclockwise and clockwise circularly polarized irradiation regions, all other conditions were the same, and circularly polarized light was directly irradiated continuously, and the difference in growth after 14 days was observed.

As a result, in this embodiment, it was confirmed that the growth of Arabidopsis upon irradiation with circularly polarized light counterclockwise are compared inhibited significantly during irradiation of the clockwise circularly polarized light. Experiments of this embodiment has Tsu multiple rows, all showed similar results. In addition, when the quarter-wave plate 28 was rotated 90 degrees to switch the left-handed and right-handed polarized light, similar data supporting the experimental result of this example was obtained. This is considered to be due to the following reasons. That is, a photoreceptor that generally senses light is composed of a protein, that is, an L-amino acid, and is an optically active substance. Experimental results of this example, the degree and efficiency of the polarized absorption of light receptors, believed to indicate different between clockwise polarized light and left-handed polarized light.
As described above, according to this example, it was found that irradiation of left circularly polarized light inhibited growth compared to irradiation of right circularly polarized light in Arabidopsis thaliana. As a result, for Arabidopsis, is irradiated with only the left circularly polarized light if you want to suppress the growth, the other hand, if you want to accelerate the growth of Arabidopsis by irradiating only the right circularly polarized light, the efficiency of development in Arabidopsis It has been found that it is possible to perform general control.

<Application field of the present invention>
Optics, biology, plant physiology, entomology, structural biology, organic chemistry, chiral optics.

<Use and application of the present invention>
According to the present invention, the behavior of a series of organisms including plant growth can be controlled by optically active light such as circularly polarized light.
For example, it can be expected that the production of vegetables and the like currently performed with multidirectional light emitting diodes can be performed more efficiently.
In addition, efficient production of crops with higher commercial value is expected, such as control of life cycle such as harvest time of crops by optically active light and production of vegetables and fruits with improved ratio of useful ingredients. Furthermore, insects and the like are generally sensitive to light, and high-efficiency and highly selective pest control according to the present invention is also expected. Application to microbial growth control technology is also expected.
By elucidating the exact acceptance mechanism of circularly polarized light, it is possible to develop a new optical switch, and it is expected to realize a biological control system using the optical switch.

1 (A) is located in one longitudinal sectional view schematically showing a configuration of a biological behavior observation apparatus according to the embodiment, i.e. longitudinal sectional view according to the cross section of the line A-A 'shown in FIG. 1 (B) of the present invention . FIG . 1B is a cross- sectional view taken along the line BB ′ of FIG. FIG. 2A is a longitudinal sectional view of the sample petri dish 40, the partition plate 42, etc., and FIG. It is a top view of the sample petri dish 40, the partition plate 42, etc. FIG. That is, FIG. ) Is a longitudinal sectional view taken along a line A-A 'in FIG. 3A is a side view of the wave plate 28 and the support plate 50, and FIG. 3B is the same wavelength. It is a top view of board 28, support board 50 grade. FIG. 4A shows a lens holder 60 as a rotation mechanism of the irradiation lens 30. 4 (B) is set at the irradiation positions of the left and right circularly polarized lights 14 and 16, respectively. It is a figure which shows the power meters 64a and 64b used.

Explanation of symbols

10 Biological Behavior Observation Device 12 Polarization Irradiation Mechanism 14 Left Circular Polarized Light 16 Right Circular Polarized Light
1 9 Light source 20 Light source 22 Condensing lens 24 Wavelength selection filter 26 Wollaston prism 28 1/4 wavelength plate (wavelength plate)
30 Irradiation lens 40 Sample petri dish
42 partition plate
44 support plate
50 support plate 54 wave plate holder (wave plate rotating mechanism)
60 irradiation lens holder ( irradiation lens rotation mechanism)

Claims (6)

In the biological behavior observation method to be used to observe the behavior of the raw material,
In order to separate the inside of the sample installation container into two left and right spaces, a partition plate is erected from above the sample installation container,
After placing one individual of the same species of organism that will exhibit the same behavior to light in the left and right spaces in the sample placement container,
Light emitted from a light source arranged above the sample installation container ,
Monochromatic light by the wavelength selection filter,
The monochromatic light is simultaneously divided into first linearly polarized light and second linearly polarized light by a prism,
The divided polarization light is converted into left and right circularly polarized light, first linearly polarized light and second linearly polarized light orthogonal to the first linearly polarized light, or left and right elliptically polarized light by a wave plate,
The partition plates separate the light of the two polarization states by the partition plate so that the light of the two polarization states generated at the same time are not mixed with each other. Of light,
A biological behavior observation method characterized by being used for observing the peculiarity of behavior exhibited by a living organism of the same species that may be caused by a difference in polarization state of light irradiated to the left and right spaces . In the biological behavior observation device used when observing the behavior of living organisms,
A sample container for placing two individuals of the same species that will behave in response to light;
A polarized light irradiation mechanism that generates a plurality of lights having different polarization states only from above the sample installation container ;
A partition plate that divides the inside of the sample installation container into two left and right spaces, and partitions the polarized lights so that a plurality of polarized lights generated from the polarized light irradiation mechanism are not mixed together;
Further, the polarized light irradiation mechanism includes a one light source that Hassu light,
A wavelength selection filter that converts the light from the light source into monochromatic light of a desired wavelength;
Polarization generation means for generating a plurality of lights having only different polarization states from monochromatic light from the wavelength selective filter,
The same kind placed in the left and right spaces in the sample installation container separated by the partition plate by partitioning the light of two kinds of polarization states generated simultaneously from the polarized light irradiation mechanism with the partition plate. Irradiate different individuals with light of different polarization states,
A biological behavior observation apparatus, which is used for observing the peculiarity of behavior exhibited by organisms of the same species that may be caused by a difference in polarization state of light irradiated to the left and right spaces . The biological behavior observation apparatus according to claim 2,
The biological behavior observation apparatus, wherein the polarization generation means includes a Wollaston prism that simultaneously splits monochromatic light from the wavelength selection filter into first linearly polarized light and second linearly polarized light. In the biological behavior observation apparatus according to claim 2 or 3,
The polarized light generating means further includes a first linearly polarized light and a second linearly polarized light simultaneously from the Wollaston prism, a left and right circularly polarized light, a first linearly polarized light and a second linearly polarized light orthogonal to the first linearly polarized light, or left and right A biological behavior observation apparatus comprising a wave plate for converting to elliptically polarized light. In the biological behavior observation apparatus according to claim 4,
A biological behavior observation device comprising a wave plate rotating mechanism that rotatably holds the wave plate around the optical axis in a plane orthogonal to the optical axis of the wave plate. In the biological behavior observation apparatus according to any one of claims 2 to 5,
The polarized light irradiation mechanism further includes an irradiation lens that irradiates two individuals of the same species of the corresponding species with a plurality of lights generated by the polarized light irradiation mechanism,
A lens rotation mechanism for holding the irradiation lens so that the optical axis of the irradiation lens can be adjusted so that the light intensity from the irradiation lens to each target site of the living organism becomes equal;
A biological behavior observation apparatus characterized by comprising:

Images