JP5350832B2 - Nerve growth promoter - Google Patents

Description

The present invention relates to a nerve growth promoter containing an extract of black ganoderma which has a nerve growth factor production promoting action and a neurite extension promoting action.

Nerve growth factor is a protein that plays an important role in differentiation and function maintenance of peripheral nerves and central nerve cells, synapse formation and repair of damage, etc., and prevents nerve cell degeneration during nerve damage It has an action (Non-patent Document 1). However, nerve growth factor is a high molecular weight protein having a molecular weight of 10,000 or more, and its administration method is restricted when it is used as a therapeutic agent, and there is a problem in terms of safety.

Accordingly, various nerve growth factor production promoters have been proposed to promote the production of nerve growth factor in vivo. For example, Fumilla mushroom genus mycelium culture supernatant (Patent Document 1), shimeji mushroom extract (Patent Document 2) and the like have been disclosed as having nerve growth factor production promoting action. However, none of them is sufficient in terms of effects.

A nerve cell has a cell body and a protrusion, and there are two types of protrusions, an axon that transmits nerve information to other nerve cells and a dendrite that receives information from other nerve cells. Nerve cells extend neurites to form a neural circuit under the influence of nerve growth factors and the influence of the external environment.
As a result, synapses are formed between nerve endings and dendrites of neurons of other neurons, and signal transmission is performed. Moreover, since the disappearance of neurites is observed in patients with memory impairment or dementia, synapse formation associated with neurite outgrowth is considered to be closely related to memory.

Promoting neurite outgrowth can reorganize and enhance synaptic responses between neurons that have failed due to various disorders, and can be expected to recover the function that has been reduced by neurodegeneration. Has been proposed.
For example, fragrance components such as citral (Patent Literature 3), peptides (Patent Literatures 4 and 5), heteropolyacids containing heavy metals such as bismuth and arsenic (Patent Literature 6) and the like are disclosed as compositions having a neurite extension promoting action. Has been. However, these have problems in terms of safety and stability.

A polyene compound has been disclosed as a substance having a nerve growth factor production promoting action and a neurite extension promoting action (Patent Document 7). Since this substance has both actions, it can be a more effective nerve growth promoter than the aforementioned nerve growth factor production promoter and neurite extension promoter. However, the polyene compound is a novel substance produced by a specific microorganism and has a problem in safety.
Under such circumstances, the development of a safe nerve growth promoter that has both a nerve growth factor production promoting action and a neurite extension promoting action has been awaited.

Black ganoderma is a mushroom belonging to the family Ganodermataceae and Ganoderma, and its scientific name is G. atrum, G. et al. japonicum, G. et al. Sinense (Non-patent Document 2). As a mushroom belonging to the genus Bamboo genus, red ganoderma (G. lucidum) is generally widely known, and Ganoderma lucidum or ganoderma is sometimes used as a common name for red ganoderma. Black ganoderma and red ganoderma are similar in shape, but black ganoderma does not contain the bitter-tasting triterpene component (ganoderic acid) peculiar to red ganoderma, so the tastes of the two are completely different (Non-patent Document 3). In addition, the appearance color of black ganoderma is dark brown, while red ganoderma is reddish brown, and both mushrooms are completely different.

Regarding black ganoderma, inventions of antihypertensive agents (Patent Document 8), antithrombotic agents (Patent Document 9), and brain function improving agents (Patent Document 10) are disclosed. However, the aforementioned antihypertensive agent is based on the inhibitory action of angiotensin converting enzyme, the antithrombotic agent is based on the platelet aggregation inhibitory action, and the brain function improving agent is based on the inhibitory action of acetylcholine transferase and prolyl endopeptidase. That is, with respect to black ganoderma, no nerve growth factor production promoting action and neurite extension promoting action according to the nerve growth promoting agent of the present application has been reported. The nerve growth factor production promoting action and neurite extension promoting action are completely different from the acetylcholine transferase inhibitory action described above.

JP 2007-230927 A JP 2007-137878 A JP2007-302572A JP 2006-42684 A JP-A-2005-239712 JP 2007-99634 A JP-A-8-319289 JP2008-255041 JP 2005-162627 A JP2008-156240

Pharmaceutical Journal, 116 (4), 286-308, 1996 Edited by Rokuya Imanoseki "Primary Color Japanese New Fungi Encyclopedia (II)", Childcare Company, 1989 Journal of Japan Brewing Association, 85 (6), 385-392, 1990

The object of the present invention is to provide a nerve growth factor-promoting action and a neurite outgrowth-promoting action, and to provide a safe nerve growth promoter, which is useful for the prevention / Contribute to treatment.

As a result of diligent research on the action of black ganoderma, the present inventors have found that the extract of black ganoderma has a high nerve growth factor production-promoting action and neurite extension-promoting action, leading to the completion of the present invention. It was.

That is, the present invention is a nerve growth promoter, comprising an extract of black ganoderma having an action of promoting nerve growth factor production and promoting neurite outgrowth. The present invention relates to a nerve growth promoter having a predetermined physical property and / or a purified product by adsorption, and a composition for cooked rice containing these nerve growth promoters.

As the black ganoderma used in the present invention, those circulated in China and the Japanese market can be used. Moreover, you may use an indigenous product and a self-cultivated product.

The black ganoderma used in the present invention can be used regardless of fruiting bodies, mycelium, natural products, cultivated products, cultured products, and the like. Moreover, the state as it is, a crushed material, a dried material, etc. can be selected suitably as needed, and it can attach to extraction operation.

The nerve growth promoting agent according to the present invention can be obtained by solvent extraction of the fruit body of black ganoderma. Examples of the solvent to be extracted include water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), polyhydric alcohols (1,3-butylene glycol, propylene glycol, Glycerin, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, petroleum ether, etc.), ethers (ethyl ether, tetrahydrofuran, propyl ether, etc.) ). Preferred are water, lower alcohols, ketones, acetonitrile, esters, and the like, and hydrous solvents of these organic solvents, and particularly preferred is water, ethanol, or hydrous ethanol from the viewpoint of safety. These solvents may be used alone or in combination of two or more. It is also preferable to extract under basic conditions.

In order to further purify the black reishi extract, there is a method in which the black reishi extract or the adsorbed purified product described later is purified by liquid / liquid partition extraction with a solvent that is not mixed with each other.

As the solvent used for liquid / liquid partition extraction, the above-mentioned solvents can be used. For example, (water or water-containing alcohol) / hydrocarbon-based, (water or water-containing alcohol) / ether, (water or water-containing alcohol) / ester, water / butanol, or the like can be used. Without being limited to these, any solvent system that does not mix with each other can be employed. Preferably, extraction is performed by adding a hydrous lower alcohol and a hydrocarbon solvent to the above-mentioned black ganoderma extract and adding an ester solvent to the residual liquid from which the hydrocarbon solvent soluble matter has been removed for extraction. A purified product can be obtained. Moreover, fractional extraction with an acid or a base can also be performed.

In order to further purify the black reishi extract, there is a method in which the black reishi extract or the aforementioned solvent fraction is adsorbed on an adsorbent and the adsorbed one is eluted with a solvent.

Examples of the adsorbent include adsorbents such as styrene-divinylbenzene synthetic adsorption resin, ion exchange resin, octadecylated silica gel, silica gel, cellulose, dextran, and modified dextran. Of these, styrene-divinylbenzene synthetic adsorption resin and octadecylated silica gel are preferable.

Examples of the adsorption method include, but are not particularly limited to, a method in which an adsorbent is packed in a column and an extract solution is passed through the column, and a method in which the adsorbent is directly mixed with the extract solution.

The elution solvent for the adsorbent can be selected depending on the characteristics of each adsorbent, and examples thereof include water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid many Monohydric alcohol (1,3-butylene glycol, propylene glycol, glycerin, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, petroleum ether, etc.) ) And ethers (ethyl ether, tetrahydrofuran, propyl ether, etc.). Solvents such as water and lower alcohols are preferable, and water and ethanol are particularly preferable. These solvents may be used alone or in combination of two or more. Furthermore, the pH may be adjusted by adding acid or alkali to the elution solvent.

The extract of black ganoderma according to the present invention contains components characterized by the following matters (hereinafter referred to as specific components of black ganoderma). The specific component can be obtained by purifying the extract of black ganoderma by the aforementioned solvent fractionation, adsorption, or a combination of solvent fractionation and adsorption.
(1) When an infrared absorption spectrum is measured (KBr method), absorption maximums are shown at 2925, 1700, 1600, 1455, 1170, and 835 cm ⁻¹ .
(2) When the above-mentioned black ganoderma biloba components are dissolved in ethanol and the ultraviolet absorption spectrum is measured, the absorption maximum is shown at 280 to 330 nm.
(3) An aromatic ring is contained in the molecule.
(4) When ethyl acetate is added to this component, the ethyl acetate-soluble solution is colored and mainly brown to reddish brown.

Among the above, (3) can be distinguished by ¹ H, ¹³ C-NMR analysis. That is, when measured using deuterated methanol (CD ₃ OD) as a solvent, spectral absorption of hydrogen atoms substituted with aromatics at 6 to 8 ppm ( ¹ H-NMR) and 100 to 150 ppm ( ¹³ C-NMR) was recognized. It is done. The above spectral analysis values may have some numerical errors depending on the analyzer, method, peak width, and the like. About (4), coloring property can be discriminate | determined by adding 1 mL of ethyl acetate to about 1-10 mg of this component.

In addition, a specific component of black ganoderma has the property of liberating hydroxycinnamic acid when hydrolyzed by a conventional method. For example, it can be hydrolyzed by a conventional method using an aqueous sodium hydroxide solution or the like, and can be confirmed by analysis with HPLC or the like.

The specific component of black ganoderma can be confirmed by high performance liquid chromatography (HPLC) shown below (see FIG. 1). The HPLC chart of the specific component of black reishi is shown. The horizontal axis represents the retention time (minutes), and the vertical axis represents the intensity. According to this analysis, a broad peak group having a retention time of about 10 to 25 minutes is a specific component of black ganoderma.
(A) Column: Octadecylated silica gel (inner diameter 4.5 mm × 250 mm)
(B) Column temperature: 40 ° C
(C) Developing solvent: 0.2% phosphoric acid / 0.2% phosphoric acid-containing acetonitrile was used, and 0.2% phosphoric acid-containing acetonitrile was linearly changed from 20% to 100% over 20 minutes. Change solvent and hold at 100% for 25 minutes (total 45 minutes analysis).
(D) Flow rate: 1 mL / min (E) Detection: 313 nm
(F) Sample: 0.02 mL of 10 mg / mL ethanol solution introduced

Black reishi extract or solvent fraction or adsorbed purified product may be used as it is, and if necessary, concentrate, dilute, filter, decolorize with activated carbon, deodorize, ethanol precipitate, etc. May be used. Further, the extracted or purified solution may be subjected to treatment such as concentration to dryness, spray drying, freeze drying, etc., and used as a dried product. Moreover, the extract and refined | purified substance of black ganoderma can be used individually or in combination.

The nerve growth promoter of the present invention may be used as an extract of Kuro Reishi or a fraction obtained by solvent fractionation or adsorption as it is, and if necessary, excipient, extender, binder, wetting agent, disintegration. An agent, a surfactant, a lubricant, a dispersant, a buffer, a fragrance, a preservative, a solubilizing agent, a solvent, and the like can be added and used. Specifically, lactose, sucrose, sorbit, mannitol, starch, precipitated calcium carbonate, heavy magnesium oxide, talc, calcium stearate, magnesium stearate, cellulose or derivatives thereof, amylopectin, polyvinyl alcohol, gelatin, surface activity Agents, water, physiological saline, ethanol, glycerin, propylene glycol, cacao butter, laurin butter, petrolatum, paraffin, higher alcohol and the like can be used.

The nerve growth promoter of the present invention can be used for any of foods, quasi drugs, and pharmaceuticals. Examples of the oral use include powders, granules, tablets, sugar-coated tablets, capsules, syrups, pills, suspensions, solutions, emulsions, and compositions for cooked rice. For parenteral use, it can be used as an injection solution or a suppository.

The intake of the nerve growth promoter of the present invention varies depending on the administration form, purpose of use, age, body weight, etc., but 0.1 to 5,000 mg / day of black ganoderma extract or solvent fraction or purified product by adsorption. Preferably, it can be administered once to several times a day in the range of 1 to 3000 mg / day. In addition, since the administration method and dose vary depending on various conditions, an amount smaller than the above-mentioned administration range may be sufficient, and administration may be performed beyond this range. In addition, the method for adding a nerve growth promoter in the preparation may be added in advance or during the production, and may be appropriately selected in consideration of workability.

The nerve growth promoter according to the present invention exhibits an excellent nerve growth factor production promoting action and neurite extension action and is highly safe.

In order to describe the present invention in detail, examples of production of the extract used in the present invention, formulation examples of the present invention, and experimental examples are given as examples, but the present invention is not limited thereto. The part of the amount shown in the examples means part by weight, and% means% by weight.

Production Example 1 Black Ganoderma Hot Water Extract 1
2 L of purified water is added to 100 g of dried black reishi fruit bodies, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate is concentrated and freeze-dried. 5.4g was obtained.

Production Example 2 50% Ethanol Extract of Black Ganoderma 1L of purified water and 1L of ethanol were added to 100g of dried ganoderma fruit bodies, extracted for 7 days at room temperature, filtered, and the filtrate was concentrated and lyophilized. As a result, 2.9 g of 50% ethanol extract of black ganoderma was obtained.

Production Example 3 Black Ganoderma Ethanol Extract 1 kg of dried ganoderma fruit body 15L of ethanol was added, extracted for 7 days at room temperature, filtered, and the filtrate was concentrated to dryness. 20 g of extract was obtained.

Production Example 4 Purified Column of Black Reishi Mushroom 10.0 g of the hot water extract of Black Reishi fruit body of Production Example 1 was dissolved in purified water and passed through a column packed with 500 mL of a styrene-divinylbenzene synthetic adsorption resin. Liquid. Next, after eluting with 1 L each of purified water, 10, 20, 50, 80, 90, and 100% ethanol, the ethanol fractions of 80% or more were combined, and each was concentrated to dryness to purify the column. About 0.40 g was obtained.

Production Example 5 Purified Solvent Fraction of Black Ganoderma 2.00 g of Kuro Ganoderma Ethanol Extract of Production Example 3 was dissolved in 350 mL of ethanol and extracted by adding the same volume of purified water and hexane. Concentrate the aqueous layer to ½, extract by adding the same volume of ethyl acetate, extract and concentrate to dryness to obtain 0.84 g of hexane fraction, 0.56 g of ethyl acetate fraction, and 0.60 g of water fraction. It was. Of these, the ethyl acetate fraction was used as a purified product of Kuro Reishi's solvent fraction.

Production Example 6 Purified HPLC Product of Black Ganoderma 0.50 g of Kuro Ganoderma Ethanol Extract of Production Example 3 was purified by preparative HPLC to obtain 0.21 g of purified Kuro Ganoderma HPLC.
<HPLC fractionation conditions>
(A) Filler: Octadecylated silica gel (inner diameter 20 mm x length 250 mm)
(B) Developing solvent: 0.2% phosphoric acid / 0.2% phosphoric acid-containing acetonitrile was used, and 0.2% phosphoric acid-containing acetonitrile was linearly changed from 20% to 100% over 20 minutes. Change solvent and hold at 100% for 25 minutes.
(C) Flow rate: 10 mL / min (D) Detection: 350 nm

Production Example 7 Solvent Fraction / Column Purified Product of Black Ganoderma 2.0 g of Kuro Ganoderma Ethanol Extract of Production Example 3 was dissolved in 350 mL of ethanol and extracted with the same volume of purified water and hexane. The aqueous layer portion was concentrated to 1/2, extracted with the same volume of ethyl acetate, and then concentrated to dryness to obtain 0.56 g of an ethyl acetate fraction. This was dissolved in 50% ethanol and passed through a column packed with 100 mL of a styrene-divinylbenzene synthetic adsorption resin. Next, after eluting 1 L each of purified water, 10, 20, 50, 80, 90, and 100% ethanol, the ethanol fractions of 80% or more were put together, and the solvent fraction / column purified product of Kuro Ganoderma was added to 0. 24 g was obtained.

<Spectral analysis of Production Examples 6 and 7>
Infrared absorption spectrum analysis Infrared absorption spectra of the HPLC purified product of Kuro Reishi in Production Example 6 and the solvent fraction / column purified product of Kuro Reishi in Production Example 7 were measured by the KBr method. As a result, the absorption maximum was shown at 3294, 2926, 1698, 1604, 1456, 1169, and 823 cm ⁻¹ .
Ultraviolet Absorption Spectrum Analysis 5 mg of the purified product of Kuro Reishi from Preparation Example 6 and the purified fraction of Kuro Reishi from Preparation Example 7 were dissolved in 100 mL of ethanol, and the ultraviolet absorption spectrum was measured. As a result, the maximum of absorption was shown at 295.3 nm.
NMR spectrum analysis As a result of ¹ H, ¹³ C-NMR analysis by a conventional method using the HPLC purified product of Kuro Reishi in Production Example 6 and the solvent fraction / column purified product of Kuro Reishi in Production Example 7, 6.6 to Spectral absorption was observed at 7.6 ppm ( ¹ H-NMR) and 100 to 150 ppm ( ¹³ C-NMR), and the presence of an aromatic ring was confirmed. Note that deuterated methanol (CD ₃ OD) was used as the solvent.
Color of Solution 5 mL of ethyl acetate was added to 10 mg of the HPLC purified product of Kuro Reishi in Preparation Example 6 and the solvent fraction / column purified product of Kuro Reishi in Preparation Example 7. After the ethyl acetate soluble component was filtered, the color of the solution was visually confirmed. As a result, the color of the solution was reddish brown.

Production Example 8 Black Ganoderma Hot Water Extract 2
Purified water of 5 kL was added to 250 kg of the dried product of Kuroshiki Shibashi fruit, extracted at 95-100 ° C. for 2 hours, filtered, and the solid content concentration was measured. Dextrin corresponding to the solid content was added, concentrated, and spray-dried to obtain 25 kg of black reishi hot water extract.

Comparative Production Example 1 Red Ganoderma Hot Water Extract To 100 g of dried red ganoderma fruit body, 2 L of purified water was added, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and frozen. After drying, 6.0 g of hot water extract of red ganoderma was obtained.

Comparative Production Example 2 Purified Solvent Fraction of Red Ganoderma The same procedure was carried out except that the black ganoder of Production Example 5 was replaced with red ganoderma turf to obtain 0.64 g of ethyl acetate fraction (purified solvent fraction).

The nerve growth promoter of the present invention can be formulated as the following formulation examples.

Formulation Example 1 Powder Formulation Formulation 1. 1. Purified HPLC product of Kuro Reishi (Production Example 6) 20 parts Dried corn starch 30
3. Microcrystalline cellulose 50
[Manufacturing method] Components 1 to 3 are mixed to form a powder.

Formulation Example 2 Tablet formulation Formulation amount 1. Solvent fraction and column purified product of Kuro Reishi (Production Example 7) 5 parts2. Dried corn starch 25
3. Carboxymethylcellulose calcium 20
4). Microcrystalline cellulose 40
5. Polyvinylpyrrolidone 7
6). Talc 3
[Manufacturing method] Components 1 to 5 are mixed, then 10% water is added as a binder, dried after extrusion granulation. Ingredient 6 is added to the molded granules, mixed and compressed into tablets. One tablet is 0.52 g.

Formulation Example 3 Tablet Confectionery Formulation amount 1. Ethanol extract of black reishi (Production Example 3) 1.5 parts Dried corn starch 50.0
3. Erythritol 40.0
4). Citric acid 5.0
5. Sucrose fatty acid ester 3.4
6). Fragrance 0.1
[Production Method] Components 1 to 4 are mixed, and 10% water is added as a binder to increase the size of the fluidized bed. Ingredients 5 and 6 are added to the formed granules, mixed and compressed into tablets. One tablet is 1.0 g.

Formulation Example 4 Beverage Formulation Black Reishi hot water extract 2 (Production Example 8) 1.0 part2. Red Ganoderma Hot Water Extract (Comparative Production Example 1) 0.3
3. Stevia 0.02
4). Maltitol 3.6
5. Gluconic acid solution (50%) 0.6
6). Fragrance 0.2
7). Water 94.3
[Production Method] Components 1 to 6 are dissolved in a part of component 7 with stirring. The remaining water of component 7 is then added and mixed.

Formulation Example 5 Cooked rice additive formulation Black Reishi hot water extract 2 (Production Example 8) 10.0 parts2. Dextrin 89.0
3. Particulate silicon dioxide 1.0
[Manufacturing Method] Components 1 to 3 are mixed to obtain a cooked rice additive. In use, 2.0 g of the additive is added to 1 rice at the time of water addition and cooked.

Next, experimental examples will be given to explain the effects of the present invention in detail.

Experimental Example 1 Nerve Growth Factor Production Promoting Action According to the method of Nakanishi et al. (Brain Res., 659, p169, 1994), primary astrocytes prepared from the cerebral cortex of 1-day-old rats are modified with Dulbecco containing 10% fetal bovine serum. A 12-well plate was seeded at a cell density of 1 × 10 ⁵ cells / well in Eagle medium (DMEM). The medium was changed every 2-3 days and cultured for 7 days. When it reached confluence, the sample was added and further cultured for 24 hours. The sample was added by preparing a DMSO (dimethyl sulfoxide) solution having a concentration 1000 times the final concentration and exchanging the medium with a solution diluted 1000 times with DMEM containing 0.5% fetal calf serum. As a control group, DMSO diluted 1000-fold with DMEM containing 0.5% fetal bovine serum was used.

After culturing, the culture supernatant was collected and measured using Promega's NGF Emax ImmunoAssay System according to the attached protocol. As the well plate, MaxiSorp manufactured by NalgenNunc was used. The measurement used 3 wells for each test group.

As shown in Table 1, black reishi extract, column purified product, solvent fraction purified product, HPLC purified product, and solvent fraction / column purified product are red ganoderma hot water extract and solvent fraction. Compared to the purified product, it showed a higher nerve growth factor production promoting effect.

Experimental Example 2 Neurite Extension Promoting Action A neurite extension promoting agent is considered to enhance the neurite forming action of nerve growth factor. Therefore, PC12 cells (obtained from RIKEN), a rat adrenal medullary pheochromocytoma-derived cell line that exhibits neuron-like behavior and is widely used as a model cell for nerves, were used for each sample. The neurite outgrowth promoting effect in the presence of growth factors was evaluated.

PC12 cells were seeded at 5 × 10 ⁴ cells / well in a poly-D-lysine-coated 24-well plate and cultured in DMEM containing 5% horse serum and 10% fetal calf serum for 2 days. Subsequently, 3 in DMEM / F-12 medium (invitrogen) supplemented with ITS (insulin <final concentration 10 μg / mL>, sodium selenite <final concentration 6.7 ng / mL>, transferrin <final concentration 5.5 μg / mL>). Washed once. The sample solution was dissolved in a DMEM / F-12 medium containing ITS (the aforementioned concentration) and NGF (mouse 7S <final concentration 10 ng / mL>) and passed through a 0.2 μm sterilizing filter. 1 mL was added. The medium was changed every two days. Five days after the sample addition, the cells were fixed with methanol, and the morphology of the cells was observed with a phase contrast microscope (100 times). Three wells were used for each treatment group. For the neurite outgrowth promoting action, the cells of each treatment group were scored according to the following criteria, and the value obtained by summing the sum of the scores of each cell by the number of cells was used as the score of each test group.
0: no neurite or neurite is shorter than cell diameter, 1: neurite is the same length as cell diameter, 2: neurite is 2-3 times longer than cell diameter, 3: neurite is More than 3 times the cell diameter

As shown in Table 2, black reishi extract, column purified product, solvent fraction purified product, HPLC purified product, and solvent fraction / column purified product are red ganoderma hot water extract and solvent fraction. Compared with the purified product, it showed a higher neurite outgrowth promoting effect.

Twenty-five males and females aged 25 to 60 years ingested 30 mL of the drink of the prescription example 4 and examined blood one month later. Decreases in triglyceride levels, total cholesterol levels, blood pressure, etc. were observed, but it was confirmed that the blood test items indicating liver function etc. were within the normal value range for all panelists and had high safety. .

  The extract of black ganoderma turf according to the present invention is a highly safe nerve growth promoter having excellent nerve growth factor production promoting action and neurite extension action and high safety. Therefore, the use as a pharmaceutical, a foodstuff, and a quasi-drug is anticipated.

The HPLC chart of the specific component of black reishi is shown. The horizontal axis represents the retention time (minutes), and the vertical axis represents the intensity.

Claims (2)

A nerve growth promoter (excluding the effect of improving brain function) characterized by containing an extract of black ganoderma which has a nerve growth factor production promoting action and a neurite extension promoting action. The nerve growth promoting agent according to claim 1, wherein the extract is a purified product obtained by solvent fractionation and / or adsorption and has the physical properties (1) to (4) (excluding the effect of improving brain function). .
(1) When the infrared absorption spectrum is measured (KBr method), the absorption maximum is shown at 2925, 1700, 1600, 1455, 1170, 835 cm ⁻¹ ,
(2) When this component is dissolved in ethanol and the ultraviolet absorption spectrum is measured, the absorption maximum is shown at 280 to 330 nm.
(3) contains an aromatic ring in the molecule,
(4) When ethyl acetate is added to this component, the ethyl acetate-soluble solution is colored and exhibits brown to reddish brown color.

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