JP5463667B2 - Topical skin preparation - Google Patents

Description

  The present invention relates to an antioxidant, an external preparation for skin and a cosmetic containing an extract of a specific plant as an active ingredient, and a cosmetic method using the cosmetic.

  In order to maintain human life, aerobic energy metabolism is carried out in vivo. During the metabolic process, active oxygen and free radicals such as superoxide, hydroxy radical, and hydrogen peroxide are removed from oxygen taken into the body. It is known to generate. The generated active oxygen and free radicals are detoxified by enzymes such as superoxide dismutase (SOD), catalase, and glutathione peroxidase. However, due to the effects of the decrease in the expression level of the enzyme due to aging and the acceleration of the generation of active oxygen and free radicals due to external stimuli, detoxification of the active oxygen and free radicals by the aforementioned enzymes becomes insufficient, and as a result, Reactive oxygen and free radicals cause cell damage and tissue damage. In particular, the skin tissue is directly stimulated by ultraviolet rays in sunlight and environmental factors such as air pollutants, so that active oxygen and free radicals are easily generated in the tissue, and various oxidative damages are likely to occur. Such oxidative damage is a major cause of pigmentation such as spots and freckles, and skin aging such as wrinkles and sagging.

  For this reason, for skin external preparations and cosmetics to protect against oxidative damage of skin tissue caused by active oxygen and free radicals, vitamin E, vitamin C and other vitamin-based antioxidants, dibutylhydroxytoluene (BHT), Attempts have been made to incorporate synthetic antioxidants such as butylhydroxyanisole (BHA). However, it may not be said that the effect of preventing oxidative injury of skin external preparations and cosmetics containing these antioxidants is sufficient, and these vitamin antioxidants are sufficiently stable under oxidizing conditions. However, synthetic antioxidants have problems such as suspected carcinogenicity.

  Therefore, a specific plant extract having an antioxidant action is selected from natural plant extracts that can be expected to have good safety for the human body, and is blended in an external skin preparation as an antioxidant. (Patent Document 1).

JP 2008-247854 A

  However, since the types of plant extracts described in Patent Document 1 are very limited, the degree of freedom in selecting plant extracts has to be reduced during the manufacture of a topical skin preparation. Some skin external preparations and cosmetics formulated with the above plant extracts may not be sufficient in preventing oxidative damage. For this reason, the new plant extract which has an antioxidant effect different from the plant extract described in patent document 1 grade is always calculated | required.

  The present invention is intended to solve the above-described problems of the prior art, and can be expected to have good safety and has an antioxidant, an antioxidant containing a new plant extract, an external preparation for skin, And to provide cosmetics.

  In view of the above-mentioned object, the present inventors have studied the effectiveness as natural antioxidants for plant-derived components that have been used for humans for a long time and are considered to have no safety problems. It has been found that a specific plant extract that has not been reported on and has no known application to external skin preparations has an antioxidant effect suitable for blending into external skin preparations and cosmetics, The present invention has been completed.

  That is, the present invention is Torikosantesu dioica (Trichosanthes dioica), Rongifuria Suwaitesu (Longifolia Thwaites), mimosa hexane Dora (Mimosa hexandra), Puremuna integrase Li cordifolia (Premna integrifolia), Afanamikisu Porisutakiya (Aphanamixis polystachya), and Anashikurusu Piresurumu (Anacyclus pyrethrum The present invention provides an antioxidant, an external preparation for skin and a cosmetic, and a cosmetic method using the cosmetic, characterized in that it contains at least one plant extract selected from the group consisting of:

  The antioxidant, external preparation for skin, and cosmetic of the present invention contain an extract of a specific plant. This plant extract can be expected to be highly safe against the skin and has a good antioxidant effect. In the cosmetic method of the present invention, the above-described cosmetic of the present invention is applied to the epidermis. Therefore, according to the present invention, it is possible to prevent or suppress the occurrence of pigmentation such as stains and freckles due to aging or sunburn, and further excellent preventive / improving effects against skin aging such as wrinkles and sagging Can be expected.

  In the present invention, as described above, an extract comprising at least one selected from the group consisting of Trichosanthes diica, Longifolia thwaites, Mimosa hexandra, Premna integrifolia, Aphanamisis polystachya, and an anaphylus pyreth as an anti-mixture. Agents, external preparations for skin, and cosmetics, and a cosmetic method using the cosmetics. First, although the above-mentioned plant is demonstrated, the production center of these plants is not specifically limited.

  Trichosanthes dioica is a perennial cucurbitaceae, cultivated widely in eastern India, and its fruit is eaten as a material for soups and stews.

  Longifolia thwaites is an evergreen tree belonging to the family Annonaceae. Cultivated throughout India and traditionally used as a treatment for fever, diabetes, hypertension, etc.

  Mimosa hexandra is a plant belonging to the family Neemaceae.

  Premna integrifolia is a plant belonging to the genus Crestidae.

  Aphanamisis polystachaya is a plant of the family Sendanidae.

  Anacyclis pyrethrum is a plant belonging to the family Asteraceae and is a perennial plant with a height of 30 cm and is also called a peritory. In Ayurveda, the root is considered a tonic and is a plant used to treat paralysis and hemorrhoids.

  Trichosanthes dioica, Longifolia thwaites, Mimosa hexandra, Premna integrifolia, Aphanamisis polystachya, shoots, stems, stems, Anaphylus pyrethrum A mixture of one part or a plurality of parts of a plant body such as seeds and roots, or one extracted from whole grass can be used. Among them, leaf extract is preferably used in the case of Trichosanthes dioica, stem extract is preferably used in the case of Longifolia ways, and whole plant extract is preferably used in the case of Mimosa hexandra. In the case of Premna integrifolia, preferably a bark extract is used, in the case of Aphanamis polystachya, preferably a bark extract is used, and in the case of Anaphylus pyrethrum, a root extract is preferably used.

  When obtaining extracts from these plants, each part may be used as it is, but after appropriate pretreatment such as drying, shredding, crushing, pressing or fermentation, the solvent is used at low or warm temperature. It is preferable to extract using. Although the extraction method is not specifically limited, For example, the method of immersing one site | part of the said plant body, or 2 or more types of site | parts in the solvent under low temperature or room temperature-heating is mentioned. The extraction time is set according to the type of extraction solvent and the extraction temperature, but is preferably about 1 to 2 weeks.

  Examples of the extraction solvent include water, lower monohydric alcohols (methyl alcohol, ethyl alcohol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (glycerin, propylene glycol, dipropylene glycol). 1,3-butylene glycol etc.), lower esters (ethyl acetate, butyl acetate etc.), hydrocarbons (benzene, hexane, pentane etc.), ketones (acetone, methyl ethyl ketone etc.), ethers (diethyl ether, tetrahydrofuran, di Propyl ether), acetonitrile, etc., and one or more of them can be used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. If necessary, purification treatment such as deodorization and decolorization may be further performed within a range not affecting the effects of the present invention. Further, if necessary, an antiseptic / antifungal agent (phenoxyethanol, paraoxybenzoates, etc.) may be added, followed by storage at low temperature for 1 to 2 days, followed by filtration.

  In the case of preparing Trichosanthes dioica, Longifolia thwaites, Mimosa hexandra, Premna integrifolia, Aphanamis polystachyya, 100% of the concentration of the alcohol used as the extraction method, or an example of the concentration of water to be used as an example. Immerse the plant in butylene glycol, extract at room temperature or warm and extract for 1 to 10 days, filter, and leave the resulting filtrate to stand for a week at low temperature or room temperature for aging, and filter again The method of performing is mentioned.

  In the antioxidant, external preparation for skin or cosmetic of the present invention, Trichosanthes dioica extract, Longifolia thwaites extract, Mimosa hexandra extract, Premna integrifolia extract, Aphanamisis polyachatya extract Is preferably 0.00001 to 10% by mass, more preferably 0.00001 to 2% by mass as a dry solid content. If the total content of these plant extracts is within this range, the plant extract can be stably blended, has high safety to the skin, and exhibits high medicinal or cosmetic effects. it can.

  Further, in the present invention, the antioxidant, the external preparation for skin, or the cosmetic is various commonly used medicinal ingredients such as a moisturizer, a whitening agent, an anti-inflammatory agent, a cell activator, a UV protection agent, a blood circulation promoter, and the like. It can be used in combination with one or more medicinal agents selected from these antioxidants. Thereby, the effect of the present invention can be further enhanced.

  Examples of humectants include aspartic acid, aspartate, alanine, arginine, alginic acid, sodium alginate, xylitol, glycine, glucose, cystine, cysteine, serine, polyethylene glycol, propylene glycol, dipropylene glycol, glycerin, polyglycerin, 1 , 3-butylene glycol, 1,2-pentanediol, hexylene glycol, xylitol, sorbitol, POE methyl glucoside, maltitol, maltose, mannitol, lysine, honey, royal jelly, chondroitin sulfate, sodium chondroitin sulfate, hyaluronic acid, hyaluronic acid Sodium, sodium acetyl hyaluronate, mucoitin sulfate, caronic acid, tranexamic acid, betaine, trehalose, chito , Urea, ceramide, atelocollagen, cholesteryl-12-hydroxystearate, sodium lactate, bile salt, dl-pyrrolidone carboxylate, soluble collagen, diglycerin (EO) PO adduct, ashitaba extract, asparagus extract , Izayoi rose extract, quince seed extract, guava leaf extract, yarrow extract, merirot extract and the like.

  Examples of the whitening agent include L-ascorbic acid, L-ascorbate, ethyl L-ascorbate, L-ascorbic acid dipalmitate, L-ascorbic acid monopalmitate, ascorbyl tetra-2-hexyldecanoate, L-ascorbic acid-2-sodium sulfate, L-ascorbic acid phosphate magnesium salt, L-ascorbic acid phosphate sodium salt, L-ascorbic acid stearate, L-ascorbic acid-2-glucoside, DL-α -Vitamin C such as tocopherol-L-ascorbic acid phosphate diester dipotassium, placental extract, kojic acid, ellagic acid, chamomile extract, fire spine extract, rensch extract, tocotrienol, glutathione, arbutin, tranexamic acid, walrus Extract, Yukinoshita extract, Acerola extract, Ages extract, Ferulic acid, Adenosine phosphate disodium, Linoleic acid, 4-n-butylresorcin, 4- (4-hydroxyphenyl) -2-butanol, 5,5 Examples include '-dipropyl-biphenyl-2,2'-diol, potassium 4-methoxysalicylate, hydroquinone, hydroquinone monobenzyl ether, pantethein-s-calcium sulfonate, oil-soluble licorice extract and the like.

  Anti-inflammatory agents include, for example, aminocaproic acid, allantoin, indomethacin, bisabolol, saponin, lysothium chloride, azulene, guaiazulene, guaiazulene sulfonate, glycyrrhizic acid and its derivatives, glycyrrhetinic acid and its derivatives, salicylic acid and its derivatives, hinokitiol, photosensitivity Examples thereof include elemental, tranexamic acid and derivatives thereof, zinc oxide, turmeric extract, ginger extract, button extract, litchi extract, and bitumen extract.

  As a cell activator, for example, aminobutyric acid, ginkgo biloba extract, fennel extract, Dutch mustard extract, carrot extract, clara extract, chlorella extract, saffron extract, soybean extract, tiso extract, nicotinic acid, Examples thereof include nicotinamide, pantothenic acid and derivatives thereof, biotin, retinol, leucine, photosensitizer, riboflavin and derivatives thereof, pyridoxine and derivatives thereof, and the like.

  Examples of UV protection agents include oxybenzone, oxybenzonesulfonic acid, tetrahydroxybenzophenone, dihydroxybenzophenone, dihydroxydimethoxybenzophenone, sodium dihydroxydimethoxybenzophenonesulfonate, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5. -Sulfonate, sinoxate, diparamethoxycinnamate mono-2-ethylhexanoate glyceryl, methyl diisopropylcinnamate, methyl methoxycinnamate, octyl paramethoxycinnamate, isopropyl dimethoxycinnamate Cinnamic acid ester mixture, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, octyl paradimethylbenzoate, paraamino Benzoic acid (PABA), ethyl paraaminobenzoate, ethylene glycol salicylate, octyl salicylate, phenyl salicylate, homomenthyl salicylate, urocanic acid, ethyl urocanate, ethylhexyltriazone, drometrizole, drometrizol trisiloxane, 4-methoxy- Examples thereof include 4′-tert-butyldibenzoylmethane, titanium oxide, talc, carmine, bentonite, kaolin, and zinc oxide.

  As a blood circulation promoter, for example, salamander extract, ginger extract, gypsum extract, chimpi extract, pepper extract, ginger extract, button extract, nonyl acid valenylamide, nicotinic acid benzyl ester, nicotinic acid β- Examples include butoxyethyl ester, capsaicin, cantalis tincture, ictamol, caffeine, tannic acid, α-borneol, tocopherol nicotinate, inositol hexanicotinate, acetylcholine, cephalanthin, and γ-oryzanol.

  Examples of other antioxidants include astaxanthin, β-carotene, γ-oryzanol, kinetin, tocopherol, dibutylhydroxytoluene, flavonoids, SOD, catalase, fullerene, phytic acid, ferulic acid, chlorogenic acid, propyl gallate, and green tea extraction , Rosemary extract, Rosehip extract, Shobu extract, Horsetail extract, Hamamelis extract, Parsley extract, Loquat leaf extract, Grapefruit extract, Citrus extract, Lychee extract, Artemisia extract, Peach Examples include leaf extract, mango extract, button pi extract, pine bark extract, platinum, ubiquinone, α-lipoic acid and the like.

  In addition to the above components, the antioxidant, skin external preparation or cosmetic of the present invention includes water, fats and oils, which are components usually used for external preparations, within a range not impairing the effects of the present invention. , Hydrocarbons, fatty acids, higher alcohols, esters, plant extracts, vitamins, water-soluble polymers, surfactants, metal soaps, alcohol, polyhydric alcohols, pH adjusters, preservatives, perfumes, Components such as powders, thickeners, pigments, chelating agents can be appropriately blended.

  The dosage form of the antioxidant, external preparation for skin or cosmetics of the present invention is not particularly limited, and examples thereof include low viscosity liquid, paste, cream, foam, emulsion, pack, ointment, powder, aerosol, patch and the like. It is done. The present invention can be applied to any of cosmetics, quasi drugs, and pharmaceuticals. Specifically, for example, it can be applied to lotions, cosmetic creams, cosmetic milks, cosmetic liquids, cosmetic packs, cosmetic cleaning agents, bath preparations, makeup cosmetics, and the like.

If the applied amount of the antioxidant, the external preparation for skin or the cosmetic of the present invention is too small, the effect of the invention cannot be obtained, and if it is too large, the effect corresponding to the added amount cannot be obtained. Therefore, it is preferably 0.0002 to 1340 μg / cm ² · day, more preferably 0.0002 to 270 μg / cm ² · day per unit area (1 cm ² ) in terms of plant extract (dry solid content). .

In addition, the cosmetic of the present invention can be preferably applied to a cosmetic method for preventing and improving skin aging for cosmetic purposes. This cosmetic method is characterized by applying the cosmetic of the present invention to the epidermis in order to prevent and improve skin aging for cosmetic purposes. In this beauty method, as a method for applying cosmetics to the epidermis, a normal beauty method such as application with bare hands or aerosol spraying can be employed. Preferred application times include after bathing and after face washing.

  The antioxidant, external preparation for skin and cosmetics of the present invention, and cosmetic method will be described more specifically with reference to preparation examples, test examples and examples, but the present invention is not limited to these. Absent.

<Preparation example of plant extract>
Trichosanthes dioica leaves, Longifolia thwaites stem, Mimosa hexandra bark, Premna integrifolia bark, Aphanamisis polystachya bark, 100 liters each in a glass, Anacythrum, liters Further, 1000 mL of a 50% aqueous ethanol solution was added and mixed and sealed. After standing at room temperature for 7 days, the insoluble matter was removed by filtration, and Trichosanthes dioica extract, Longifolia thwaites extract, Mimosa hexandra extract, Premna integrifolia extract, Aphanamisis polyschalypas extract, and Aphanamisis polysphatya extract I got each. This extract was used as a plant extract in the following test examples and examples.

<Test Example> Antioxidant Action Test Trichosanthes dioica, Longifolia thwaites, Mimosa hexandra, Premna integrifolia, Aphanamisis polystachylu, and Phenyla polyisata extract from Trichosanthes dioica, Longifolia thwaites A radical scavenging experiment using -picryl-hydrazyl (1,1-diphenyl-2-picrylhydrazyl: DPPH) was performed as described below.

That is, the antioxidant effect was evaluated by measuring the decrease in absorbance at 520 nm caused by the reduction of DPPH, which is a stable radical substance having a maximum absorption at 520 nm, by the antioxidant contained in the extract. Specifically, each plant extract was diluted to 2 vol% with phosphate buffered saline (PBS), and 50 μL of the resulting diluted solution was added to a 96-well microplate. Thereto, 50 μL of a 2.5 × 10 ⁻⁴ mol / L DPPH ethanol solution was added and mixed well, and then allowed to stand at room temperature in a dark place for 30 minutes. Finally, the absorbance was measured with an absorbance plate reader (Tecan, Infinite 200F) using a 520 nm filter (half width 10 nm). In addition, the absorbance was measured in the same manner using a reaction solution in which only ethanol not containing DPPH was added instead of the DPPH ethanol solution as a blank solution. The numerical value measured by the above method was substituted into the following equation to calculate the antioxidant rate. The obtained results are shown in Table 1.

In the above formula, A is the absorbance of the reaction solution containing the plant extract, B is the absorbance of the blank solution containing the plant extract, C is the absorbance of the reaction solution not containing the plant extract, and , D is the absorbance of the blank solution containing no plant extract.

  From Table 1, an antioxidant effect was recognized in the reaction solution containing the extract of a specific plant. Therefore, it turns out that each plant extract of Table 1 is an effective antioxidant.

Examples 1 to 6, Comparative Example 1 (Preparation of topical cream)
Among the blended components in Table 2, the components (1) to (6) are heated and mixed to prepare a mixture 1A at 75 ° C. Separately, the components (7) to (10) are added to the component (12). A mixture 1B at 75 ° C. was prepared by heating and mixing. The resulting mixture 1A and mixture 1B were mixed and emulsified using a homomixer and allowed to cool to room temperature to prepare mixture 1C. Component (11) was uniformly mixed with the obtained mixture 1C to obtain an external cream.

Example 7 and Comparative Example 2 (Preparation of lotion)
Among the blending components in Table 3, components (1) to (6) are mixed and dissolved at room temperature to prepare mixture 2A. Separately, components (7) to (13) are mixed and dissolved at room temperature. Mixture 2B was prepared. The obtained mixture 2A and mixture 2B were uniformly mixed using a stirrer to obtain a skin lotion. Moreover, the skin lotion of the comparative example 2 was manufactured like Example 7 except having replaced with the Longifolia thwaites extract and using purified water.

Example 8 (Preparation of emulsion)
Among the blending components in Table 4, the components (1) to (8) are heated and mixed to prepare a mixture 3A at 75 ° C. Separately, the components (9) to (13) are added to the component (15). A mixture 3B at 75 ° C. was prepared by heating and mixing. The resulting mixture 3A and mixture 3B were mixed and emulsified using a homomixer and allowed to cool to room temperature to prepare mixture 3C. Component (14) was uniformly mixed with the obtained mixture 3C to obtain an emulsion. Further, an emulsion of Comparative Example 3 was produced in the same manner as Example 8 except that purified water was used in place of the Mimosa hexandra extract.

Example 9 and Comparative Example 4 (Preparation of pack agent)
Among the blending components in Table 5, the mixture (1) to (6) is heated and dissolved at 70 ° C. while mixing to prepare a mixture 4A. Separately, the components (7) to (10) are mixed at room temperature. The mixture 4B was prepared by mixing and dissolving. The obtained mixture 4A and mixture 4B were mixed using a stirrer and allowed to cool to room temperature to obtain a pack agent. Moreover, the pack agent of the comparative example 4 was manufactured like Example 9 except using purified water instead of a Premna integrifolia extract.

<Evaluation test>
About the external cream shown in Examples 1 to 6 or Comparative Example 1 (prepared by substituting the plant extract of the present invention in the formulation of Example 1 with purified water), the effect of improving skin roughness was tested as described below. evaluated.

<Roughness improvement effect evaluation method>
40 female subjects (25 to 60 years old) with marked skin roughness were divided into two groups in half, and each of the creams of Example 1 and Comparative Example 1 was given to each group twice daily (when washing in the morning) After bathing at night), the face was used for 3 months, and the degree of improvement in rough skin before and after use was observed by a beauty technician and evaluated according to the following evaluation criteria. The results obtained are shown in Table 6. The numbers in Table 6 are the number of subjects corresponding to the item.

(Evaluation criteria)
Evaluation rank: Content A (effective): Rough skin was improved.
B (somewhat effective): Rough skin was slightly improved.
C (invalid): Improvement of rough skin is not observed.

  As is clear from Table 5, when the external creams of Examples 1 to 6 containing specific plant extracts were used for rough skin, the results were more prominent than when the cream of Comparative Example 1 was used. A rough skin improving effect was observed.

  Moreover, when the lotion, milky lotion, and pack agent of Examples 7 to 9 were evaluated in the same manner as in Examples 1 to 6, the skin roughness more remarkable than the lotion, emulsion, and pack agent of Comparative Examples 2 to 4, respectively. Improvement effect was observed.

  Furthermore, in the above evaluation test, no subject complained of skin disorders such as skin irritation and allergic reaction in the group using any of the examples.

  As mentioned above, it turns out that the cosmetics of the Example of this invention have the improvement effect excellent with respect to rough skin, and high safety | security compared with the corresponding comparative example.

  The antioxidant, external preparation for skin and cosmetics of the present invention have excellent antioxidant action, are safe and stable against oxidation, and thus are suitable for application as cosmetics. In addition, it can prevent and improve skin oxidative damage, exhibit its effectiveness in rough skin and skin aging, and protect the skin. Therefore, the antioxidant, external preparation for skin, cosmetics, and beauty method of the present invention are useful in the medical field and the beauty field.

Claims (4)

Antioxidant characterized by blending an extract of b Ngifuria Suwaitesu (Longifolia thwaites). Skin external agent characterized by blending an extract of b Ngifuria Suwaitesu (Longifolia thwaites). B Ngifuria Suwaitesu (Longifolia Thwaites) cosmetic characterized by blending an extract.   A cosmetic method comprising applying the cosmetic according to claim 3 to the skin.