JP5677927B2 - Deodorant composition - Google Patents

Description

  The present invention relates to a deodorant composition using a plant extract.

  Bad breath is mainly caused by volatile sulfur compounds such as methyl mercaptan, which causes discomfort to many people. Conventionally, various deodorants have been developed and proposed to remove bad breath. Among these, various deodorizers using plant extracts have been proposed in consideration of safety to the human body (for example, see Patent Documents 1 and 2).

  Further, as a deodorant having a strong deodorizing power and can be used safely, a deodorant (see Patent Document 3) using a plant extract as an active ingredient of bad breath and an oxidoreductase in combination with a specific plant extract and phenol A deodorant (see Patent Documents 4 and 5) in combination with an enzyme that oxidizes an ionic compound has been proposed.

JP-A-57-204278 JP-A 61-240960 JP-A 63-309269 JP 10-212221 A JP 2010-158375 A

  However, in the above-described conventional technology using a plant extract, many plant extracts that can be used are exemplified. However, when a plant extract is used in combination, the deodorizing effect particularly excellent for methyl mercaptan Combinations showing are not illustrated.

  This invention is made | formed in view of the said situation, The main objective provides the deodorant composition which shows the especially outstanding deodorizing effect with respect to methyl mercaptan, and the foodstuff containing this composition. There is.

  The inventors of the present invention have found that among plant extracts having a deodorizing effect on methyl mercaptan, the combined use of Vulgaris extract and Thyme extract synergistically improves the deodorizing effect on methyl mercaptan, and the present invention completed.

That is, the gist of the present invention is as follows.
[1] A deodorant composition containing an extract of moths and thyme,
[2] The deodorant composition according to [1], further containing peroxidase,
[3] A food containing the deodorant composition according to [1] or [2].

  ADVANTAGE OF THE INVENTION According to this invention, the deodorant composition which shows a synergistic deodorizing effect with respect to methyl mercaptan can be provided.

It is a graph which shows the relationship between the compounding ratio of both components, and a deodorizing rate in the deodorizer composition containing a mulberry extract and a thyme extract. It is a graph which shows the relationship between the compounding ratio of both components, and a deodorizing rate in the deodorizing agent composition containing a mulberry extract and a strawberry tea extract. It is a graph which shows the relationship between the compounding ratio of both components, and a deodorizing rate in the deodorizer composition containing a strawberry tea extract and a thyme extract. It is a graph which shows the relationship between the mixing ratio and the deodorization rate of a mulberry extract and a thyme extract in a deodorant composition containing a mulberry extract, a thyme extract, and a peroxidase. It is a graph which shows the relationship between the compounding ratio and deodorization rate of a jellyfish extract and a green tea extract in the deodorant composition containing a jellyfish extract, a green tea extract, and a peroxidase.

  The deodorant composition of the present invention is characterized in that it contains a mulberry extract and a thyme extract as a plant extract exhibiting a deodorizing effect.

  Vulgaris are evergreen broad-leaved trees of the beech family and thyme is a perennial plant of the family Lamiaceae. In the case of obtaining an extract from these raw material plants, the use site of the raw material plant is not particularly limited as long as it includes a portion showing a deodorizing effect on methyl mercaptan. For example, leaves, branches, trunks, stems, roots, fruits , Flowers, seeds, bark and the like.

  The plant extract used for this invention is manufactured by extracting the said raw material plant using an extraction solvent. The extraction solvent is not particularly limited, but a polar solvent is usually preferable, and examples thereof include water, lower aliphatic alcohols, and mixtures thereof. Examples of the lower aliphatic alcohol include ethanol, propanol, isopropanol, butanol, 1,3-butylene glycol, ethylene glycol, propylene glycol, and isoprene glycol. Of the above, water is preferred. When water-containing lower aliphatic alcohol is used as the extraction solvent, the amount of alcohol contained therein can be 10 to 90% by weight.

  The water used as the extraction solvent is not particularly limited. For example, pure water, tap water, well water, or those subjected to various treatments such as ion exchange, membrane treatment, filtration, heat sterilization, distillation, and pH adjustment should be used. Can do.

  Various extraction methods can be employed as appropriate for the extraction conditions depending on the properties of the raw plant. As an example, there may be mentioned a method of adding 3 to 20 times by weight of a polar solvent to a dried raw material plant leaf or twig ground material, impregnating or stirring, and performing extraction. The extraction time is preferably 30 minutes to 1 day, and the extraction temperature is preferably room temperature to 100 ° C.

  Although the extraction operation may be performed once, the extraction operation can be performed again by adding a polar solvent to the residues of the raw material plants collected after extraction, such as leaves and twigs. Extracts obtained by a plurality of extraction operations can be combined as one extract, or any one of the extracts can be used as the extract.

  The form of the plant extract obtained from the raw material plant is not particularly limited. For example, in addition to the extract itself, a diluted solution of the extract, a concentrated solution, a lyophilized product, or a purified product that has undergone fractionation or purification treatment, etc. Can be mentioned.

  In addition, when extracting a raw material plant with a polar solvent, a nonpolar solvent (for example, hexane, benzene, toluene, etc.) may be added beforehand, and a fat-soluble component may be dissolved in a nonpolar solvent and removed.

  The extract obtained above can be subjected to purification treatment such as activated carbon treatment, adsorption resin treatment, ion exchange resin treatment alone or in combination, as appropriate, in order to further enhance the functionality. Moreover, in order to handle the said extract easily, processings, such as formulation, can be given.

Even when used alone, the jellyfish extract and thyme extract obtained by the above-mentioned method show a deodorizing effect on methyl mercaptan, but by using both in combination, a synergistic deodorizing effect is shown. . The mixing ratio of the two is not particularly limited because it shows a synergistic deodorizing effect in any case, but in order to further increase the synergistic effect, the ratio of Vulgari extract / Thyme extract = 3/7 to 7/3 by weight ratio. It is preferable to set the range.

  The deodorant composition according to the present invention can contain peroxidase in order to enhance the deodorizing effect on methyl mercaptan in addition to the above-mentioned two kinds of plant extracts. Peroxidase can be extracted from cruciferous horseradish (Armoracia rusticana), cruciferous radish (Rahpauns acanthiformis) or cucumber cucumber (Cucumis sativus), or fungi (Alternaria, Aspergillus oryzae, Coprinus cinereus, Oidiodendron) From a culture solution of Bacillus), it is obtained by extraction with water from cold to room temperature, or concentrated and purified from cold to room temperature. Since peroxidase is easily available, it is preferable to use a commercially available product.

  The deodorizing effect on methyl mercaptan is achieved by blending a very small amount of peroxidase with respect to the total weight of the above two kinds of plant extracts. Specifically, the amount of peroxidase to the plant extract is preferably 0.001 to 1, more preferably 0.001 to 0.1, and more preferably 0.001 to 0.1 as the peroxidase / plant extract (weight ratio). 01 is particularly preferred. When the peroxidase / plant extract (weight ratio) is less than 0.001, the deodorizing effect on methyl mercaptan is hardly exhibited. On the other hand, if the peroxidase / plant extract (weight ratio) exceeds 1, the cost increases, which is not preferable.

  In the deodorant composition of the present invention, for the purpose of preventing dental caries, in addition to plant components and peroxidase, a glucosyltransferase inhibitor, a plaque formation inhibitor, an oral cavity that generates caries, periodontal disease, etc. Antimicrobial agents, anti-inflammatory agents or other deodorants against pathogenic bacteria can be included.

  Examples of the glucosyltransferase inhibitor and the plaque formation inhibitor include radish, sorghum, burdock, lobster, elephant, turmeric, shinyi, kouka, sesame, pheasant, licorice, gentian, senna, assembly, glaze, cocoon, kempasi, loquat, Examples include various extracts such as Maikai, Hermanus, Tamarind, Orange, Eucalyptus, Jujube, Grape Seed, and Grape Leaf.

  Antibacterial agents against oral pathogenic bacteria include, for example, cetylpyridinium chloride, triclosan, benzalkonium chloride, benzethonium chloride, cocophosphatidyl-dimonium, trichlorocarbanide, zinc pyrithione, isopropylmethylphenol, applephenone, hinokitiol, polylysine, green tea Examples include extracts, catechins, mulberry bark extracts, ginkgo biloba extracts, thymol, methyl salicylate, eugenol, 1,8-shioner, menthol and the like.

  As an anti-inflammatory agent, for example, Asenyak, Vulture, Ogon, Licorice, Psycho, Hawthorn, Perilla, Glaze, Mulberry white skin, Kyonin, Taiso, Clove, Tonin, Nutmeg, Mace, Thyme, Thyme, Buttonpi, Azulene, Allantoin, Glycyrrhizin, And tranexamic acid.

  Examples of other deodorants include cinnamon, clove, nutmeg, mace, perilla, ginkgo biloba, cocoon leaf, oolong tea, copper chlorophyllin sodium, maltol and the like.

  In addition to the above-mentioned optional components, the deodorant composition of the present invention contains known additives such as surfactants, binders, wetting agents, sweeteners, preservatives, and fragrances, depending on the application. It can be included. Examples of the surfactant include sucrose fatty acid esters. Examples of the binder include carboxymethyl cellulose, hydroxyethyl cellulose, sodium alginate, carrageenan, gum arabic and the like. Examples of the wetting agent include polyethylene glycol, sorbitol, glycerin, propylene glycol and the like. Examples of the sweetener include saccharin, stevioside, glycyrrhizin, thaumatin, aspartame, acesulfame K, sucralose and the like. Examples of the preservative include dehydroacetic acid, benzoic acid, and parabens. Examples of the fragrances include menthol, menthone, carvone, eugenol, thymol, anethole, mint oil, peppermint oil, spearmint oil, eucalyptus oil, ginger oil, anise oil and the like.

  The deodorant composition of this invention is normally mix | blended as an additive raw material of foodstuffs. The kind of food is not particularly limited, and examples thereof include confectionery (for example, chewing gum, gummi, candy, tablet confectionery, etc.), film-like food, and the like. The content of the deodorant composition in the food can be appropriately changed according to the release rate when ingested, the dissolution concentration, the palatability, etc., and is not particularly limited, usually 0.0001 to 10% by weight, preferably 0.005 to 1% by weight.

  Hereinafter, the present invention will be described in more detail with reference to test examples and the like, but the present invention is not limited thereto.

1. Production Examples of Plant Extracts In conducting the deodorization test, the following four types of plant extracts were produced.
(Example of production of the extract of Vulgaris)
Distilled water (300 g) was added to 50 g of dry pulverized leaves containing vegetative twigs, and the mixture was heated to reflux for 2 hours. The obtained extraction mixture was allowed to stand at room temperature and cooled, followed by filtration to separate into an extract and a residue. Distilled water (300 g) was again added to the residue, and heating under reflux, cooling and filtration were performed under the same conditions as described above. Extraction operation was performed 3 times in total, and the extract obtained by each operation was combined into one, and then concentrated to dryness under reduced pressure to obtain a velvet extract.

(Production example of thyme extract)
300 g of 50% (v / v) hydrous alcohol was added to 30 g of dried thyme leaves, and the mixture was heated to reflux at 80 ° C. for 3 hours. The obtained extraction mixture was allowed to stand at room temperature and cooled, followed by filtration to separate into an extract and a residue. To the residue was again added 300 g of 50% (v / v) hydrous alcohol, and the mixture was heated under reflux, cooled and filtered under the same conditions as above. Extraction operation was performed 3 times in total, and the extract obtained by each operation was combined into one, and then concentrated to dryness under reduced pressure to obtain a thyme extract.

(Production example of green tea extract)
300 g of distilled water was added to 50 g of green tea leaves, and the mixture was heated to reflux at 90 ° C. for 2 hours. The obtained extraction mixture was allowed to stand at room temperature and cooled, followed by filtration to separate into an extract and a residue. Distilled water (300 g) was again added to the residue, and heating under reflux, cooling and filtration were performed under the same conditions as described above. Extraction operation was performed 3 times in total, and the extract obtained by each operation was combined into one, and then concentrated to dryness under reduced pressure to obtain a green tea extract.

(Manufacturing example of strawberry tea extract)
300 g of 50% (v / v) water-containing alcohol was added to 30 g of roughly dried dried tea leaves, and heated to reflux at 80 ° C. for 2 hours. The obtained extraction mixture was allowed to stand at room temperature and cooled, followed by filtration to separate into an extract and a residue. To the residue was again added 300 g of 50% (v / v) hydrous alcohol, and the mixture was heated under reflux, cooled and filtered under the same conditions as above. Extraction operation was performed 3 times in total, and the extract obtained by each operation was combined into one, and then concentrated to dryness under reduced pressure to obtain a strawberry tea extract.

2. Deodorization test 1 (deodorant composition comprising a plant extract)
Example 1 Deodorant Composition Containing Vulgaris Extract and Thyme Extract First, 20 ml of 0.1M phosphate buffer was added to a sealable 100 ml vial to simulate the oral condition The variegated extract and thyme extract were dissolved at a blending ratio shown in Table 1 to a total of 0.0025% (w / v). Subsequently, 0.2 ml of a 50 ppm methyl mercaptan solution was added. Immediately seal the vial and incubate each test sample at 37 ° C. for 30 minutes, then aspirate 50 ml of gas in the head space in the vial with the Kitagawa gas detector tube (Mercaptans, No. 130U) The methyl mercaptan concentration (ppm) in the space was measured.
In addition, when the maggot extract and the thyme extract were each used alone, the methyl mercaptan concentration (ppm) in the headspace in the vial was measured in the same manner as described above.
The methyl mercaptan concentration (ppm) in the head space in the vial was measured in the same manner as described above, using the test group to which only the phosphate buffer was added without adding the plant extract as above, and deodorized by the following formula The rate (%) was calculated. Table 1 shows the measurement results.

Deodorization rate (%) = (C−S) / C × 100
C: Methyl mercaptan concentration (ppm) in the head space of the control test zone
S: Concentration of methyl mercaptan (ppm) in the head space of the deodorant addition test section

  FIG. 1 is a graph showing the relationship between the mixing ratio of the extract of mulberry extract and thyme extract shown in Table 1 and the deodorization rate. From FIG. 1, the deodorization rate when using the above-mentioned two kinds of plant extracts together is the deodorization rate of two points when each is used alone (0:10 and 10: 0 on the horizontal axis of FIG. 1). The deodorizing effect is improved by the synergistic effect of the combined use of the above two kinds of plant extracts. In addition, the said straight line shows the case where the deodorizing effect of the deodorant composition containing the above-mentioned two kinds of plant extracts appears as the sum of the deodorizing effect according to the blending ratio of each component (that is, the additive effect) It is a virtual additive straight line attached assuming that

(Comparative Example 1) Deodorant Composition Containing Vulture Extract and Boiled Tea Extract Of the plant extract used in the above "(Example 1) Deodorant Composition Containing Vulture Extract and Thyme Extract" Among them, the deodorization test was conducted in the same manner as “(Example 1) Deodorant composition containing jellyfish extract and thyme extract” except that the tea extract was used instead of the thyme extract. It was. That is, the deodorant composition which consists of the extract of a moth white extract and a strawberry tea extract was used for the deodorization test. The results are shown in Table 2 and FIG.

  From FIG. 2, the deodorization rate when using a Vulgari extract and a strawberry tea extract as a plant extract is the deodorization rate of two points when each is used alone (0:10 on the horizontal axis of FIG. 2 and The deodorization rate at 10: 0) almost overlaps with the virtual additive line connecting the above two kinds of plant extracts, but the synergistic effect of the combined use is not shown, and additive deodorization is not shown. It only showed the effect.

(Comparative example 2) Deodorant composition containing strawberry tea extract and thyme extract Plant extract used in the above-mentioned "(Example 1) Deodorant composition containing moth extract and thyme extract" Of these, except that the tea extract was used in place of the extract of moths, the deodorization test was conducted in the same manner as "(Example 1) Deodorant composition containing jellyfish extract and thyme extract". I did it. That is, a deodorant composition composed of strawberry tea extract and thyme extract was subjected to a deodorization test. The results are shown in Table 3 and FIG.

  From FIG. 3, the deodorization rate when the tea extract and thyme extract are used together as the plant extract is the deodorization rate of two points when each is used alone (0:10 on the horizontal axis of FIG. 3 and The deodorization rate at 10: 0) almost overlaps with the virtual additive line connecting the above two kinds of plant extracts, but the synergistic effect of the combined use is not shown, and additive deodorization is not shown. It only showed the effect.

3. Deodorization test 2 (deodorant composition using both plant extract and peroxidase)
(Example 2) Deodorant Composition Containing Vulture Extract, Thyme Extract, and Peroxidase First, in order to simulate the condition in the oral cavity, a 0.1 M phosphate buffer solution was placed in a sealable 100 ml volume vial. Add 20 ml, dissolve the extract of radish extract and thyme extract to a total of 0.0025% (w / v) at the mixing ratio shown in Table 4, then peroxidase (from horseradish manufactured by Oriental Yeast Co., Ltd.) (Specific activity: 450 U / mg) was added to the phosphate buffer so as to be 0.000005% (w / v). Subsequently, 0.2 ml of a 50 ppm methyl mercaptan solution was added. Immediately seal the vial and incubate each test sample at 37 ° C. for 30 minutes, then aspirate 50 ml of gas in the head space in the vial with the Kitagawa gas detector tube (Mercaptans, No. 130U) The methyl mercaptan concentration (ppm) in the space was measured.
In addition, a deodorant composition composed of either a mulberry extract or thyme extract and peroxidase was treated in the same manner as described above, and the methyl mercaptan concentration (ppm) in the head space in the vial was measured.
The methyl mercaptan concentration (ppm) in the head space in the vial was measured in the same manner as described above using the test group to which only the phosphate buffer was added without adding the plant extract, and the above-mentioned “2. The deodorization rate (%) was calculated in the same manner as in “Odor test 1 (deodorant composition comprising plant extract)”. Table 4 shows the measurement results.

  FIG. 4 is a graph showing the relationship between the deodorization rate and the compounding ratio of the moth extract and thyme extract shown in Table 4. From FIG. 4, the deodorization rate when using the above-mentioned two kinds of plant extracts together is the deodorization rate of two points when each is used alone (0:10 and 10: 0 on the horizontal axis of FIG. 4). The deodorizing effect is improved by the combined use of the above two kinds of plant extracts, because of the convexity above the virtual additive line connecting the deodorization rate). .

(Comparative Example 3) Deodorant Composition Containing Vulture Extract, Green Tea Extract, Peroxidase Used in the above-mentioned “(Example 2) Decontaminant Composition Containing Rot Extract, Thyme Extract, Peroxidase” The same method as “(Example 2) deodorant composition containing urajirogashi extract, thyme extract, peroxidase” except that green tea extract was used instead of thyme extract among plant extracts. The deodorization test was conducted. That is, the deodorant composition which consists of an extract of a mulberry leaf, a green tea extract, and a peroxidase was used for the deodorization test. The results are shown in Table 5 and FIG.

  FIG. 5 is a graph showing the relationship between the mixing ratio of the extract of green moth extract and green tea extract shown in Table 5 and the deodorization rate. From FIG. 5, the deodorization rate when using the Vulture extract and green tea extract as plant extracts is the deodorization rate of two points when each is used alone (0:10 on the horizontal axis in FIG. 5 and The deodorization rate at 10: 0) almost overlaps with the virtual additive line connecting the above two kinds of plant extracts, but the synergistic effect of the combined use is not shown, and additive deodorization is not shown. It only showed the effect.

4). Production Examples of Chewing Gum Chewing gum was produced according to the following Formulation Example 1, Formulation Example 2 and Formulation Example 3. As described later, the deodorizing effect of the obtained chewing gum extract, the flavor and texture when the gum was eaten investigated.

<Formulation example 1> (Product of the present invention)
Vulture extract 0.007g
0.003g thyme extract
Peroxidase 0.0001g
Gum base 35g
Calcium carbonate 2g
Maltitol 55g
Xylitol 5g
Fragrance for gum 1g

<Formulation example 2> (Comparative product)
Vulture extract 0.01g
Peroxidase 0.0001g
Gum base 35g
Calcium carbonate 2g
Maltitol 55g
Xylitol 5g
Fragrance for gum 1g

<Formulation 3> (Comparative product)
0.01g thyme extract
Peroxidase 0.0001g
Gum base 35g
Calcium carbonate 2g
Maltitol 55g
Xylitol 5g
Fragrance for gum 1g

5. Deodorization test of chewing gum In order to examine the effect of the chewing gum obtained in the above "4. Production example of chewing gum", the deodorizing effect of the chewing gum extract was examined. First, the chewing gums of Formulation Example 1, Formulation Example 2 and Formulation Example 3 were each chopped and powdered by a pulverizer, and then 50 ml of 0.1 M phosphate buffer (pH 7.2) was added to 10 g of each powdered chewing gum. And extracted at room temperature for 1 hour, followed by extraction at 37 ° C. for 10 minutes. Next, the extraction mixture was subjected to solid-liquid separation by filtration to obtain each chewing gum extract. 20 ml of each chewing gum extract was added to a 100 ml capacity sealable vial and 0.2 ml of 50 ppm methyl mercaptan solution was added. Immediately seal the vial and incubate each test sample at 37 ° C. for 30 minutes, then aspirate 50 ml of the gas in the vial with a Kitagawa gas detector tube (Mercaptans, No. 130U) and add methyl in the head space. The mercaptan concentration was measured. The methyl mercaptan concentration (ppm) in the head space in the vial was measured in the same manner as described above, with the test group to which only the above phosphate buffer was added without adding the deodorant, and the deodorization rate was calculated according to the following formula: (%) Was calculated. Table 6 shows the measurement results.

Deodorization rate (%) = (C−S) / C × 100
C: Methyl mercaptan concentration (ppm) in the head space of the control test zone
S: Methyl mercaptan concentration (ppm) in the headspace of the test section using the chewing gum extracts of Formulation Examples 1 to 3

  First, in this test, the blending ratio of the plant extract in the chewing gums of blending examples 1 to 3 is constant (0.01% by weight). And from Table 6, the deodorization rate of the mixing example 1 became larger than the deodorizing rate of the mixing example 2 and the mixing example 3. It can be said that the above results are consistent with the results shown in FIG. Therefore, it can be seen that the deodorizing effect on methyl mercaptan is improved by the synergistic effect of the combined use of both the extract of mulberry extract and thyme extract in food.

6). Flavor and Texture Six panelists engaged in flavoring the chewing gums of Formulation Example 1, Formulation Example 2 and Formulation Example 3 were sampled four times without notifying the chewing gum content and sample timing. As a result, the chewing gums of Formulation Example 1, Formulation Example 2 and Formulation Example 3 could not be distinguished. From this, it was found that there is no influence on foods by using both the extract of moths and thyme.

  INDUSTRIAL APPLICABILITY The present invention can be widely used as a deodorant composition that exhibits an excellent deodorizing effect on methyl mercaptan, and is suitable, for example, as a food additive material.

Claims (3)

  A deodorant composition containing an extract of moths and thyme.   The deodorant composition according to claim 1, further comprising peroxidase.   A food containing the deodorant composition according to claim 1 or 2.

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