JP5682121B2 - Petri dish for preparation of fractionation medium - Google Patents

Description

  The present invention relates to a petri dish for preparing a divided medium in which the medium fraction of the petri dish is fractionated into two or more, and more specifically, a medium in which a convex part and a concave part are formed on the upper surface of the bottom part to be filled with the medium. The present invention relates to a petri dish for preparing a fractionation medium in which peeling of the composition is effectively prevented.

  The petri dish is configured as a set of two very shallow cylindrical containers with slightly different diameters so as to be nested. If these are called a petri dish body and a lid part, respectively, the lid part is slightly larger and shallower than the petri dish body, and the petri dish body is slightly smaller and deeper than the lid part. Since the upper end of the petri dish body is processed so as to be parallel to the bottom part, when the petri dish body with the lid part is left still, the petri dish body and the lid part are in close contact with each other by its own weight, so that it is properly sealed It has become. In addition, it is common that the culture medium filled in the petri dish main body is stored with the lid portion turned to the mounting table side by inverting the petri dish after the lid portion is mounted after filling. This is because it is possible to avoid various bacteria from falling on the surface of the culture medium.

  A petri dish can be used by filling a medium for culturing microorganisms. However, if one petri dish is used for culturing one microorganism, a plurality of petri dishes for different mediums are required. For this reason, for the purpose of saving labor, a fractionation medium that can be divided into a plurality of compartments by providing a partition inside the petri dish body, and each fraction can be filled with the same or different medium for the purpose of saving labor A petri dish for creation has been developed.

However, if the petri dish body for preparing the fractionation medium is filled with the medium, and then stored while the petri dish is turned upside down, the medium may dry and the medium may peel from the petri dish body.
On the other hand, a petri dish for preparing a fractional medium capable of preventing such a fall of the medium has also been developed. For example, the inside of the petri dish body is divided into two by a partition wall disposed in the center thereof, and the bottom surface portion near the partition wall, the bottom surface portion near the side wall and / or the partition wall in the petri dish for preparing the fractionation medium There is one in which a peeling prevention means made of a protrusion is formed on the part (Japanese Patent No. 2627142). In Patent Document 1, in the case where a plurality of fractions are formed by a partition wall, the height of the partition wall is set in consideration of the fact that the tip of the platinum ear contacts the partition wall and hinders inoculation work when the partition wall protrudes from the medium. It is characterized in that it is restricted to be lower than the height, and further, a peeling prevention means is added. The specific structure of the peeling preventing means is a band-like protrusion protruding in a convex shape from the bottom surface of the petri dish body, and the partition wall of the petri dish for preparing the fractionation medium or the vicinity thereof, and / or the petri dish body for preparing the fractionation medium Is provided on the side wall or in the vicinity thereof. In addition, as a partition which divides | segments the petri dish main body for fractionation medium preparation into two or more small fractions, in addition to the single plate-shaped object (FIG. 3) formed protruding from the bottom face part of the petri dish main body, patent document 1 FIG. 8 and FIG. 9 also disclose a sectional view and an arch-shaped partition wall that partitions each fraction.

  There is also a petri dish for preparing a fractional culture medium, in which the upper surface of the bottom surface of each compartment is continuously depressed at the base of the partition wall and the inner wall surrounding it and the bottom surface is flat (Patent Document 2). It is said that when the continuous depression is formed at the base of the partition wall and the inner wall, the separation of the medium is remarkably suppressed unlike the fractionation medium in which the depression is discontinuously formed.

Japanese Patent No. 2627142 Japanese Patent No. 4204644

  The petri dish for preparing a fractionation medium disclosed in the above-mentioned Patent Document 1 and Patent Document 2 is formed with a recess or a protrusion on the bottom surface of the petri dish body for preparing a fractionation medium, and the medium is filled in the recess or the protrusion. Thus, peeling is prevented. However, even the petri dish for preparing a fractional medium disclosed above does not sufficiently prevent the exfoliation of the medium.

The reason for this is not clear, but it is presumed that the petri dish body made of synthetic resin is likely to be deformed such as warpage and distortion, and the medium is easily peeled off by such deformation. For example, a petri dish for preparing a fractionation medium disclosed in Patent Document 1 and Patent Document 2 is manufactured by injection molding using a transparent synthetic resin, and the resin inlet is the center of the bottom surface. In this aspect, the partition is formed so as to pass through the upper part of the resin injection port, and when the left and right are gripped with the partition as the center, the shape is symmetrical and the side wall of each fraction is easily distorted. The medium is easily peeled off.

  The patent document 1 is characterized in that the inoculation operation is improved by making the height of the partition wall partitioning the culture medium lower than the height of the culture medium. In this case, even if the side wall is distorted and a convex portion is formed in the vicinity of the partition wall or the side wall, this distortion may easily eliminate the fit between the culture medium and the convex portion, and the culture medium may be peeled off.

  Further, it is considered that the exfoliation of the medium occurs not only due to distortion of the petri dish body but also due to vibration from filling the medium to use. For example, when inoculating a petri dish main body filled with a culture medium and also when inserting into an incubator after inoculation, vibration is generated due to friction between the bottom surface of the petri dish main body and the mounting table, and peeling is caused by such vibration. Is likely to occur. Further, when the lid portion is attached to the petri dish body, if the sizes of the outer periphery of the petri dish body and the inner periphery of the lid portion are greatly different, vibration is likely to occur when the petri dish body and the lid portion come into contact with each other. Such vibrations contribute to the peeling of the medium.

  Furthermore, a petri dish for preparing a fractionation medium is intended to save labor and space by filling a single dish with a plurality of culture media. During storage, a plurality of petri dishes for preparing a fractionation medium can be stacked stably and compactly. It is preferable that it is possible. In addition, it is preferable that generation of vibrations can be avoided also in the case of stacking during such storage or use.

In view of the above-mentioned present situation, an object of the present invention is to provide a petri dish for preparing a fractional medium that can prevent the exfoliation of the medium.
Another object of the present invention is to provide a petri dish for preparing a fraction medium that can stably and easily stack a plurality of petri dishes for preparing a fraction medium.

As a result of examining the exfoliation of the culture medium in the petri dish for preparing the fractionation medium in detail, the present invention forms a recess in the bottom surface portion in the vicinity of the partition wall that is divided into a plurality of fractions of the petri dish body for preparing the fractionation medium, and the side wall If the convex portion is formed on the bottom surface near the plate, it is possible to avoid the peeling of the medium more efficiently than when all the concave portions are formed, and avoid the distortion and vibration during operation of the petri dish body for preparing the fractionated medium. As a method of avoiding such distortion, it is possible to prevent the culture medium from peeling off, and as a method of avoiding such distortion, the petri dish body is provided by asymmetrically arranging the concave portion and the convex portion, and the foot portion formed on the lower surface of the bottom surface portion. In addition, the partition wall is not plate-shaped, but an arch-shaped partition wall formed by connecting the tops of two small partition walls to improve the strength of the petri dish for preparing the fractionation medium. Efficient distortion during storage Reduced it found such that can prevent the peeling of the medium, thereby completing the present invention.

That is, the present invention comprises a bottom surface portion, a side wall disposed on the outer periphery of the bottom surface portion, and a partition wall that divides the bottom surface portion into a plurality of small fractions and is connected to the side wall. The present invention provides a petri dish body for preparing a fractional culture medium, wherein a concave portion provided in the vicinity of the partition wall and a convex portion provided in the vicinity of the side wall are formed.

Moreover, the said recessed part and / or convex part provide the petri dish main body for a fraction culture medium preparation divided | segmented into 2-20 pieces.
In addition, the petri dish body for preparing a fractional culture medium having a depth of the recess of 0.3 to 3 mm, more preferably 0.5 to 2.5 mm is provided.

In addition, the petri dish body for preparing a fractional culture medium having a height of the convex portion of 0.5 to 3 mm, more preferably 1.0 to 2.5 mm is provided.
Moreover, the said recessed part and / or convex part provide the petri dish main body for a fraction culture medium characterized by being formed asymmetrically in the bottom face part of the petri dish main body for a fraction culture medium preparation.

  Further, the partition wall disposed in the petri dish body is an arch type in which the small partition walls constituting the small fraction are continuously arranged at the top, and provides the petri dish body for preparing the fractionation medium. .

  In addition, the two small partition walls constituting the arch type have a lower end portion connected in the vicinity of and / or the center of the side wall of the fractionation medium preparation petri dish body, and the fractionation medium preparation petri dish is characterized in that The body is provided.

In addition, the petri dish body for preparing a fractionation medium is provided in which the height of the partition wall is higher than the center near the side wall.
Further, the petri dish body provides the petri dish body for preparing a fractional culture medium, wherein a foot is formed on the lower surface of the bottom surface.

Furthermore, the above-mentioned foot is provided with a petri dish body for preparing a fractionation medium, characterized in that it is formed discontinuously.
Furthermore, the petri dish body for preparing a fractional culture medium is provided, wherein the convex part and the foot part are arranged so as to fill the gap between the convex parts and between the foot parts. It is.
Furthermore, the petri dish for fractionation medium preparation which consists of the said petri dish main body for fractionation medium preparation and a cover part is provided.

  The lid portion includes a top surface portion and a side wall disposed on the outer periphery of the top surface portion, and the top surface portion is formed with a rim height portion that can be fitted to an upper end of the side wall of the petri dish body. A petri dish for preparing a fractionation medium is provided.

In addition, the present invention provides a petri dish for preparing a fractional culture medium, wherein a plurality of ribs are formed inside the edge height portion.
Further, the present invention provides a petri dish for preparing a fractional culture medium characterized in that a protrusion is formed on the upper surface of the edge height portion of the lid.

Moreover, the said protrusion part provides the petri dish for fractionation-medium preparation characterized by being comprised discontinuously.
In addition, the petri dish for preparing the above-mentioned fractionation medium having corners formed by arcs is provided.

Further, the present invention provides a fractionation medium obtained by filling the petri dish for preparing the fractionation medium with a medium.

  According to the petri dish for preparing a fractional culture medium of the present invention, since the concave portion and the convex portion are formed on the bottom surface portion of the petri dish body, the culture medium is also filled in the concave portion and the convex portion when the medium is filled therein. In combination, the exfoliation of the medium is efficiently suppressed.

  In addition, when the petri dish for preparing a fractionation medium of the present invention is a plurality of small fractions each having a bottom surface and a side wall provided through the upper end of the side wall, the strength of the petri dish body is improved, so the petri dish body Warpage and distortion at the time of use can be suppressed, and peeling of the medium can be efficiently suppressed.

  In addition, the petri dish for preparing the fractionation medium of the present invention has a plurality of fractionation media when the lid part is formed with an edge height part that can be fitted to the upper end of the side wall of the petri dish body and the foot part is formed on the bottom part of the petri dish body. When the petri dish for preparation is laminated on the upper side, the bottom face part and the edge height part are combined, and the lamination width can be reduced and stored in a space-saving manner.

  In addition, the petri dish for preparing a fractionation medium of the present invention can reduce the contact surface with the mounting table and suppress vibration due to friction when the foot is formed in the vicinity of the outer periphery of the bottom surface of the petri dish body. . For this reason, if a culture medium is filled in this, the peeling of the culture medium which arises by vibration can be suppressed efficiently.

In addition, the petri dish for preparing a fractionation medium of the present invention can be manufactured by injection molding, is easy to manufacture, and can be manufactured at low cost.
The fractionation medium of the present invention is obtained by filling the above-described petri dish for preparing the fractionation medium with a medium, and in which peeling of the medium due to vibration during storage or operation is suppressed.

FIG. 1 is a petri dish for preparing a fractionation medium of the present invention, and is a diagram for explaining a state in which a lid is attached to the petri dish body of the present invention. FIG. 1 (b) is a side view of the petri dish for preparing a fractionation medium (300), and FIG. 1 (c) is a bottom view of the petri dish for preparing the fractionation medium (300). FIG. 2 is a diagram for explaining an example of a preferred embodiment of the petri dish body (100) for preparing the fractionation medium of the present invention. 2A is a plan view of the petri dish body 100, FIG. 2B is a right side view, FIG. 2C is a front view, and FIG. 2D is a bottom view. FIG. 3 is a partial cross-sectional view of the petri dish body (100) shown in FIG. FIG. 4 is a view for explaining the recess (40) formed in the petri dish body (100) of the present invention, and is a partial cross-sectional view taken along the line CC of FIG. 2 (a). FIG. 5 is a view for explaining the partition wall (30) formed in the petri dish body (100) of the present invention, and is a partial cross-sectional view taken along the line DD in FIG. 2 (a). FIG. 6 is a diagram for explaining a convex portion (50) formed on the petri dish body (100) of the present invention, and FIG. 6 (a) is a partial cross-sectional view taken along the line AA of FIG. FIG. 6B is a partial cross-sectional view taken along the line BB in FIG. FIG. 7 is a view for explaining a mode in which the partition wall (30) formed on the petri dish body (100) of the present invention is connected to the side wall (20), and the petri dish body of the present invention shown in FIG. 100) is a partially enlarged view of the flat plane view of. FIG. 8 is a view for explaining the relationship between the partition wall (30) and the side wall (20) formed in the petri dish body (100) of the present invention, and FIG. 8 is a partial sectional view taken along line AA of FIG. is there. FIG. 9 is a diagram for explaining the relationship between the protruding portion (25) and the partition wall (30). FIGS. 9 (a), 9 (b), and 9 (c) are views of FIG. They are a partial sectional view of a line, a partial sectional view of a CC line, and a partial sectional view of a DD line. FIG. 10 is a partially enlarged view of the bottom view of the petri dish body (100). FIG. 11 is a diagram illustrating another embodiment of the petri dish body (100) of the present invention, in which a partition wall (30) composed of two small partition walls (31) is directly connected to the side wall (20). . 12 is a view for explaining a mode of connection between the partition wall (30) and the side wall (20) of the petri dish body (100) shown in FIG. 11, and is a partial cross-sectional view taken along line AA of FIG. FIG. 13 shows another embodiment of the petri dish body (100) according to the present invention, in which a partition wall (30) composed of two small partition walls (31) is recessed from the side wall (20) inward (25 ′). ) And a partial enlarged view of a plan view of the petri dish body (100). FIG. 14 is a diagram for explaining a mode of connection between the partition wall (30) and the side wall (20) of the petri dish body (100) shown in FIG. 13, and is a partial cross-sectional view taken along line AA in FIG. FIG. 15 shows that a partition wall (30) composed of a small partition wall (31) is united in the vicinity of the side wall (20) to form a protrusion (25 ′), and the protrusion (25 ′) is a bottom (10). It is a figure explaining the aspect which does not exist in the cavity formed in the partition (30) continuously. FIG. 16 shows another embodiment of the petri dish body (100) of the present invention, and is a diagram for explaining that a plate-like protrusion (25a) is formed on the upper part of the protrusion (25, 25 ′). FIG. 17 is another embodiment of the petri dish body (100) of the present invention, and is a diagram for explaining that a plate-like protrusion (25a) is formed on the upper part of the protrusion (25, 25 ′). 17 (a) is a partial cross-sectional view taken along line AA in FIG. 16 (a), and FIG. 17 (b) is a partial cross-sectional view taken along line AA in FIG. 16 (b). FIG. 18A is the same petri dish body (100) as FIG. 2A, and FIG. 18B is a partial cross-sectional view taken along the line AA of FIG. Figure 19 is a diagram bottom part illustrating the manner provided continuously to each other of the small partition wall in the center of the laboratory dish body (100) of the present invention (31), FIG. 19 (a) FIG. 2 (a ) Of FIG. 19B is a partial cross-sectional view taken along line E-E of FIG. 19B, and FIG. 19B is a partial cross-sectional view taken along line FF of FIG. FIG. 20 is an explanatory view showing the relationship between the foot ( 60 ), the partition wall (30), and the convex portion (50) in the petri dish body (100) of the present invention. FIG. 21 is a view for explaining the foot portion (60) formed on the bottom surface portion (10) of the petri dish body (100) of the present invention, and is a partial cross-sectional view taken along the line GG of FIG. FIG. 22 is a diagram for explaining an example of a preferred embodiment of the lid (200) constituting the petri dish for preparing a fractionated medium of the present invention, FIG. 22 (a) is a plan view, and FIG. 22 (b) is a left side. FIG. 22C is a right side view, FIG. 22D is a front view, and FIG. 22E is a bottom view. FIG. 23 is a view for explaining the edge height portion (230) of the lid portion (200) used in the present invention, and is a partial cross-sectional view taken along line AA in FIG. 22 (a). The petri dish body (100) when the lid part (200) is mounted is indicated by a broken line, and the petri dish body for preparing the fractionation medium (100) in the case where a plurality of fractionation medium preparation petri dishes are stacked is provided on the lid part (200). Shown in broken lines at the top. FIG. 24 is a diagram for explaining the protrusion (240) and the edge height (230) formed on the lid (200) used in the present invention, and FIG. 24 is a BB line in FIG. 22 (a). It is a partial enlarged view of the vicinity. FIG. 25 is a partially enlarged view of the vicinity of the line CC in FIG. 22 (e), and is a view for explaining the rib (235a) and the rib (235b) formed inside the edge height portion (230). FIG. 26 is a cross-sectional view taken along the line B-B of FIG. 22A, and the rib (235a) and the rib (235b) formed inside the edge height portion (230) of the lid portion (200) used in the present invention. FIG. FIG. 27 is a diagram illustrating the fractionation medium of the present invention, and is a diagram illustrating the relationship between the medium height and the partition wall (30) height.

The first of the present invention comprises a bottom surface portion, a side wall disposed on the outer periphery of the bottom surface portion, a partition wall that divides the bottom surface portion into a plurality of small fractions and that is connected to the side wall, A recess provided near the partition and a convex provided near the side wall are formed on the bottom surface, and a discontinuous foot is formed near the outer periphery of the bottom surface. , the said foot mutual, between the convex portion is disposed is fractionated media creation laboratory dish body, characterized in Rukoto to fill the gap between the foot portion.

The second of the present invention is a petri dish for preparing a fractionation medium comprising the above-mentioned petri dish body for preparing a fractionation medium and a lid.
Furthermore, the third aspect of the present invention, the fractionation media creation Petri dish, a fraction medium formed by filling the culture ground. Hereinafter, the present invention will be described in detail with reference to the drawings.

(1) Petri dish body for preparing a fractionation medium The petri dish for preparing a fractionation medium (300) of the present invention comprises a petri dish body for preparing a fractionation medium and a lid. FIG. 1 is a plan view, a bottom view, and a side view of an example of a petri dish (300) for preparing a fractional culture medium according to the present invention in a state where a lid (200) is attached to the petri dish body (100). FIG. 1 (a) is a plan view of a petri dish for preparing a fractionation medium. The top surface portion (210) of the lid portion (200), the side wall (220) disposed on the outer periphery thereof, and the top surface portion (210). A part of the edge height (230) formed in the vicinity of the side wall (220) and a protrusion (240) formed discontinuously on the edge height part (230) are shown. FIG. 1 (b) is a side view of the petri dish for preparing a fractionation medium (300), and shows a mode in which a lid (200) having a larger diameter and shorter than that is attached to the petri dish body (100). FIG. 1 (c) is a bottom view of the petri dish (300) for preparing the fractionation medium. The bottom part (10) of the petri dish body (100), the side wall (20) disposed on the outer periphery thereof, and the side wall The foot part (60) formed discontinuously in the vicinity of (20) is shown. In addition, the partition part (30) divided | segmented into two or more divisions and the recessed part (40) are formed in the bottom face part (10) in the vicinity. However, FIG. 1 is an exemplification, and the present invention is not limited to FIG.

FIG. 2 is a diagram illustrating the petri dish body (100) shown in FIG. 2A is a plan view of the petri dish body 100, FIG. 2B is a right side view, FIG. 2C is a front view, and FIG. 2D is a bottom view. The left side view is symmetrical with the right side view, and the rear view is symmetrical with the front view.

(I) Separation The petri dish body (100) for preparing the fractionation medium of the present invention has a side wall (20) formed on the outer periphery of the bottom surface portion (10), and the top surface of the bottom surface portion (10) is defined by the partition wall (30). Is divided into at least two sub-fractions. In the present invention, the “partition wall” means that the bottom surface portion (10) is divided into a plurality of small fractions and connected to the side wall. For example, in FIG. 2A, the bottom surface portion (10) is divided into two small portions by the partition wall (30) formed at the center of the bottom surface portion (10). Although it is divided into strokes (α, β), four small fractions may be formed by dividing walls arranged in a cross, and in addition, a plurality of small fractions are formed by dividing walls arranged radially. It may be.

  The shape of the “partition wall” is not limited, and it may be a single plate or other shapes. FIG. 3 shows a partial cross-sectional view of the petri dish body (100) shown in FIG. The bottom surface portion (10) is raised to form two small partition walls (31), and the top portion of the small partition wall (31) is connected in an arch shape to form one partition wall (30). The partition wall (30) divides the bottom surface portion (10) into two to form a small fraction (α) and a small fraction (β). The partition wall (30) formed by connecting the tops of the small partition walls (31) in an arch shape is excellent in strength, has little bending and distortion during use, and can effectively avoid peeling of the culture medium. Moreover, if it is an arch type, since the center part of a partition (30) is hollow, a weight can be reduced.

The height of the partition wall (30) can be appropriately selected depending on the size of the petri dish for preparing the fractionation medium and the amount of medium to be filled. If the diameter of the petri dish for preparing the fractionation medium is 10 cm, for example, in FIG. 3, the height (h1) of the partition wall is 2.5 to 10 mm, more preferably 2.5 to 5 mm. In addition, the height (h1) of the partition wall (30) does not have to be uniform in the petri dish body for preparing the fractionation medium.

(Ii) Concave part In this invention, the recessed part (40) is formed in the vicinity of the partition (30) of a bottom face part (10), It is characterized by the above-mentioned. When the concave portion (40) is formed in the vicinity of the partition wall (30), the peeling of the culture medium can be efficiently prevented in combination with the convex portion (50) formed in the vicinity of the side wall (20).

  One or more concave portions (40) may be present on the bottom surface portion (10), but preferably one or more, and more preferably two or more in each small fraction. FIG. 2 (a) shows a mode in which two concave portions (40) are formed in each small fraction (α, β) at equal intervals. More preferably, the number is 2 to 20, more preferably 2 to 10, and particularly preferably 2 to 5 in each small fraction.

  In the present invention, the recesses need not be arranged in the same number or the same shape in each subfraction. For example, when the concave portion (40) is disposed asymmetrically with the partition wall (30) as the center, the bottom surface portion (10) is less distorted, so that the function of preventing the peeling of the medium is effectively exhibited.

  FIG. 4 is a partial cross-sectional view taken along line CC in FIG. The bottom surface portion (10) is depressed in a linear shape to form a recess (40). As described above, when the petri dish has a diameter of 10 cm, the depth (d1) of the recess (40) is 0.3 to 3 mm, more preferably 0.5 to 2.5 mm. Moreover, although there is no restriction | limiting in the length (L1) of a recessed part (40), Preferably, the full length of a recessed part (40) is 50-100% of the length of a partition (30), More preferably, it is 60-90%. Prepare as follows. Even when the distortion of the bottom surface portion (10) occurs in this range, the exfoliation of the medium can be efficiently suppressed after the medium is filled.

FIG. 5 is a partial cross-sectional view taken along the line DD in FIG. Concave portions (40) are formed on both sides adjacent to the partition wall (30).
In the present invention, it is preferable that all of the corners of the concave portion (40) are arcs. If the corner portion of the recess (40) is an arc, it is possible to suppress the generation of bubbles when the medium is filled, and to prevent cracking from the corner portion after the medium is solidified.

(Iii) Convex part In this invention, as shown to Fig.2 (a), a convex part (50) is formed in the bottom face part (10) of the inner periphery vicinity of a side wall (20), It is characterized by the above-mentioned. It was found that even when the petri dish body (100) is distorted in combination with the recesses (40) formed in the vicinity of the partition wall (30), the culture medium can be prevented from peeling off.

  In the present invention, at least one convex portion is formed on the bottom surface portion (10), but more preferably, it is 1 or more, and more preferably 2 or more, for each small fraction. FIG. 2A shows a mode in which three long convex portions (50) are formed in each of the small fractions (α, β).

  More preferably, the number of convex portions (50) is 2 to 20, more preferably 2 to 10, and particularly preferably 2 to 5 in each subfraction. In addition, the said convex part (50) does not need to be arrange | positioned by the same number or the same shape in each subfraction. For example, in the petri dish body (100) shown in FIG. 2 (a), the convex portions (50) are arranged symmetrically about the partition wall (30). You may arrange asymmetrically with two etc. in a picture. As in the case of the concave portion (40), when the convex portion (50) is configured to be asymmetrical left and right, it is possible to efficiently avoid distortion that occurs evenly when the petri dish body (100) is gripped from the left and right. Because you can. In addition, it is preferable to arrange | position a convex part (50) so that the top part may be arrange | positioned within 3 mm from a side wall (20) so that the identification property of a colony may not be affected.

  FIG. 6A shows a partial cross-sectional view taken along line AA in FIG. The height (h2) of the convex portion (50) is 0.5 to 3 mm, more preferably 1.0 to 2.5 mm. The width (w1) is 0.3 to 1.0 mm, more preferably 0.4 to 0.8 mm. Within this range, the distortion of the bottom surface portion (10) can be avoided, and after filling the culture medium, irregularities can be formed on the bottom surface portion of the culture medium, and the separation can be efficiently suppressed.

It is preferable that all the corners of the convex part (50) are formed by arcs. If the corners of the protrusion (5 0) is a circular arc it is possible to suppress the occurrence of air bubbles at the time of the medium filling, and after the culture medium solidified can be suppressed cracks from the corners.

FIG. 6B is a partial cross-sectional view taken along the line BB in FIG. The aspect by which a bottom part (10) protrudes linearly and a convex part (50) is formed is shown. As described above, the corners of the bottom surface and the top are all arcs. In the present invention, the length (L2) of the convex portion (50) may be the same between the bottom surface portion and the top portion, but as shown in FIG. 6 (b), the top portion is made shorter than the bottom surface portion. May be. In addition, although there is no restriction | limiting in the length (L2) of a convex part (50), Preferably, the full length of a convex part (50) is 30 to 90% of the perimeter of a petri dish main body (100), More preferably, 50- 90%.

(Iv) Protruding part FIG. 7 shows a partially enlarged view of the petri dish body (100) of the present invention shown in FIG. In the present invention, the partition wall (30) is connected to the side wall (20). At this time, as shown in FIG. 7, a part of the side wall (20) forms a protrusion (25). A part (25) and a partition (30) may be connected.

  FIG. 8 is a partial cross-sectional view taken along line AA in FIG. In the figure, for the purpose of describing each component, the boundary between the protruding portion (25) and the other components is indicated by a broken line for convenience. A part of the side wall (20) protrudes inside the petri dish body (100) to form a protrusion (25), and the protrusion (25) is connected to the partition wall (30). The height of the protrusion (25) shown in the figure increases from the connecting portion with the partition wall (30) toward the side wall (20). When the height of the projecting portion (25) is increased in the vicinity of the side wall (20), the contact area with the side wall (20) is increased, the strength of the petri dish body is increased, and deformation such as bending and distortion can be prevented. .

  Furthermore, in order to explain the relationship between the partition wall (30), the concave portion (40), the bottom surface portion (10), and the protruding portion (25), a partial cross-sectional view taken along the line BB of FIG. , DD sectional views are shown in FIGS. 9 (a), 9 (b) and 9 (c), respectively. As shown in FIGS. 9B and 9C, the partition wall (30) is configured such that the top of the small partition wall (31) is connected in an arch shape, so that a cavity is formed in the center. . On the other hand, the small partition walls (31) constituting the partition wall (30) approach each other in the vicinity of the side wall, unite, and are connected to the protruding portion (25) shown in FIG. For this reason, the cavity part formed in the lower center of a partition (30) does not exist in the vicinity of a side wall (20). When the partition wall (30) is composed of a small partition wall (31), the deformation easily occurs, such as spreading from the left and right around the top of the partition wall (30) due to the presence of the hollow portion, but in the vicinity of the side wall (20). Since the two small partition walls (31) are connected to each other, the occurrence of the distortion can be efficiently avoided. FIG. 9 (b) shows a mode in which a recess (40) having a bottom surface (10) depressed is formed adjacent to the partition wall (30).

  In the present invention, it is preferable that the protruding portion (25) is also formed with a circular arc at the corner. This is because the shock caused by vibration is alleviated by the arc and the separation of the medium is efficiently prevented. In addition, in FIG. 10, the elements on larger scale of the bottom view of a petri dish main body (100) are shown. As for the edge part of a partition (30), the corner | angular part is formed in the circular arc.

  On the other hand, the connection of the partition wall (30) and the side wall (20) is not limited to the embodiment shown in FIG. For example, as shown in the partially enlarged view of FIG. 11, the partition wall (30) composed of two small partition walls (31) may be directly connected to the side wall (20). FIG. 12 is a partial sectional view taken along line AA in FIG.

  In the present invention, as the protrusion (25) that forms the partition wall (30) on the side wall (20), a part of the side wall (20) is further recessed and protruded inside the petri dish body (100). (25 ′). The recessed protrusion (25 ′) may also be configured to be higher than the partition wall (30) in the vicinity of the side wall (20), as in FIG. FIG. 13 shows a partially enlarged view of a plan view of the petri dish body (100) provided with such a depression type protrusion (25 ′). Unlike the case of FIG. 7, the lateral width of the projecting portion (25 ′) is different from that in FIG. 7 because the side wall (20) is depressed to form the projecting portion (25 ′) while maintaining the same thickness as the side wall (20). It extends to the same width as (30).

  FIG. 14 is a partial sectional view taken along line AA in FIG. In FIG. 14, the side wall (20) is recessed inward to form a protrusion (25 ′), and the lower end of the protrusion (25 ′) is connected to the bottom surface (10). This means that, as in FIG. 8, the ends of the two small partitions (31) are united in the vicinity of the side wall (20), and there is no cavity formed in the center of the partition (30). means. Accordingly, even when the protruding portion (25 ′) recessed from the side wall (20) is formed and connected to the partition wall (30), the strength of the petri dish body (100) is improved and distortion is efficiently avoided. Can do. For the sake of explanation, a cross-sectional view taken along the line BB in FIG. 13 is shown in FIG. In the vicinity of the side wall (20) of the petri dish body (100), there is no cavity of the bottom surface portion (10) generated when the small partition walls (31) are connected.

  In addition, in this invention, a partition (30) divides | segments a bottom face part (10) into several small fractions, and is connected with the said side wall. The projecting portion (25) is formed of a part of the side wall (20). However, the bottom portion (10) is divided into a plurality of small fractions and connected to the side wall (20). It shall be included in the concept.

  In addition, the shape of the said protrusion part (25) is not limited above, For example, the aspect shown to Fig.16 (a) and FIG.16 (b) may be sufficient. 7 and 13 is that a plate-like protrusion (25a) is further formed on the upper part of the protrusion (25, 25 '). FIG. 17 shows a partial cross-sectional view of these AA lines. 17A is a partial cross-sectional view taken along line AA in FIG. 16A, and FIG. 17B is a partial cross-sectional view taken along line AA in FIG.

By forming the plate-like protrusion (25a), the connecting area between the partition wall (30) and the side wall (20) can be improved, the strength of the petri dish body (100) can be improved, and distortion during use can be avoided. it can.

(V) Injection injection port The petri dish body (100) of the present invention can be manufactured by injection molding. At that time, the injection inlet is preferably formed at the lower end of the partition wall (30). This is because the visibility of the colony is not affected. FIG. 18A shows the same petri dish body (100) as FIG. 2A, and FIG. 18B shows a partial cross-sectional view taken along the line AA of FIG. 18A. As shown in FIG. 18 (a), a partition wall (30) composed of a small partition wall (31) is formed on the diameter of the bottom surface portion (10), and an injection injection port (15) is connected to the partition wall (30). Has been. In this invention, it is preferable to form in the lower end part of a partition (30), and the center part of a petri dish main body. As a result, the hollow portion of the arch-shaped partition wall (30) is filled with the resin, and the lower ends of the two small partition walls (31) are molded together even in the central portion of the petri dish body, and the culture medium generated due to distortion or the like is peeled off. Can be suppressed.

  That is, as described above, in the present invention, the side wall (20) and the partition wall (30) are connected via the protrusion (25), and at this time, the lower end portions of the two small partition walls (31) are united. Is done. Furthermore, by arranging the injection injection port (15) in the center of the petri dish body, the lower end portions of the two small partition walls (31) can be molded together even in the center portion. Can be more effectively suppressed.

FIG. 19A shows a partial cross-sectional view taken along line EE in FIG. The bottom surface portion (10) is raised to form an arch-shaped partition wall (30) composed of two small partition walls (31), and the bottom surface portion of the small partition wall (31) is made of resin injected into the injection injection port (15). The cavity is filled with resin. Moreover, the fragmentary sectional view of the FF line of Fig.2 (a) is shown in FIG.19 (b).

(Vi) Foot part The petri dish body (100) preferably has a foot part (60) formed on the bottom surface of the bottom face part (10). In the petri dish body (100) shown in FIG. 2 (d), four legs (60) are formed evenly. By forming the foot portion (60), friction between the bottom surface portion (10) and the mounting table is reduced, and peeling of the culture medium due to vibration can be avoided.

  Further, the number and length of the foot portions (60) are not particularly limited, and may be, for example, a continuous ring-shaped foot portion formed along the side wall (20), and is divided into a plurality of discontinuities. It may be made of a protrusion. Further, for example, the foot (60) may be arranged symmetrically left and right or up and down around the partition wall (30), or may be arranged asymmetrically. When the foot (60) is disposed asymmetrically, distortion during use and storage can be avoided more efficiently.

  In the present invention, for example, as shown in FIG. 20, the bottom surface portion (10) spans the gap between the convex portion (50) and the convex portion (50) formed on the upper surface of the bottom surface portion (10). ) Or continuously at the connecting portion between the partition wall (30) and the side wall (20) so as to extend over the two small partition walls (31). The convex portion (50) on the upper surface of the bottom surface portion (10) and the foot portion (60) on the lower surface of the bottom surface portion (10) are filled with the gap between the convex portions (50) and the foot portions (60). By being arranged, deformation of the petri dish body (100) can be suppressed.

FIG. 21 is a partial cross-sectional view taken along the line GG in FIG. In the present invention, the height (h3) of the foot (60) is 0.2 to 3 mm, more preferably 0.5 to 2 mm.
In addition, in this invention, it is preferable that the said corner | foot part (60) is also formed in a corner | angular part in circular arc. If the top is an arc, the friction with the mounting table can be further reduced.

(Vii) Side Wall In the present invention, a side wall is formed on the outer periphery of the bottom surface portion (10). As shown in FIG. 21, a bulge portion (23) is formed at the upper end of the side wall (20), and the corner portion of the bulge portion (23) is formed in an arc. When the bulging portion (23) having a circular arc shape is formed at the upper end of the side wall (20), the contact area with the lid portion (200) is reduced. It can be reduced, and scratches due to vibrations can be avoided.

(2) Lid The petri dish body for preparing a fractionation medium of the present invention constitutes a petri dish for preparing a fractionation medium together with a lid. As the lid portion, any of the lid portions constituting a conventionally known petri dish can be used.

(I) Edge height part In this invention, as shown to the top view of Fig.22 (a), as a cover part (200), it arrange | positions in the outer periphery of the top surface part (210) and the said top surface part (210). Preferably, the top surface portion (210) is formed with an edge height portion (230) capable of supporting the upper end of the side wall (20) of the petri dish body (100). be able to. When the upper end of the side wall (20) of the petri dish body (100) is attached to the edge height portion (230) formed on the lid portion (200), the movement of the lid portion (200) during use is reduced. It is possible to suppress the generation of scratches that are likely to occur due to impact. 22B is a left side view of the lid portion 200 that can be suitably used in the present invention shown in FIG. 1, FIG. 22C is a right side view, FIG. 22D is a front view, and FIG. 22 (e) is a bottom view. The rear view is the same as the front view.

FIG. 23 is a partial sectional view taken along line AA in FIG. The edge height portion (230) formed on the lid portion (200) used in the present invention is preferably a continuous ring-shaped edge height portion (230) disposed in the vicinity of the side wall portion (220). Incidentally, in FIG. 23, reference numeral 240 is a collision raised portion which will be described later. Further, the petri dish body (100) when the lid part (200) is attached is indicated by a broken line, and the fraction culture medium preparation petri dish body (100) when a plurality of fractionation medium preparation petri dishes are stacked is the lid part (200 ) Is indicated by a broken line.

The top surface portion (210) of the lid portion (200) is relatively recessed by the edge height portion (230) formed in the vicinity of the outer periphery of the lid portion (200), and another petri dish body (100) is placed in the recessed portion. When placed, the foot portion (60) comes into contact with the recessed portion of the top surface portion (210) and is stably laminated. The width (w2) of the edge height portion (230) is 1.5 to 5 mm, more preferably 2 to 4 mm. If it is the said range, since the width | variety of the upper end of the side wall (20) of a petri dish main body (100) is generally 0.8-1.5 mm, a petri dish main body will be in the edge high part (230) of a cover part (200). The side wall (20) of (100) can be easily mounted, and the lid (200) can be prevented from shaking. Moreover, the petri dish body (100) can be stably laminated on the top surface part (210). In order to avoid friction, it is preferable that the bottom surface portion (10) of the petri dish body (100) does not contact the top surface portion (210) of the lid portion (200). Therefore, it is preferable to adjust the depth of the edge height portion (230) of the lid portion (200) to be shallower than the height (h3) of the foot portion (60).

(Ii) Projection Part In the present invention, the projection part (240) may be formed on the upper surface of the top surface part (210) of the lid part (230). FIG. 22A shows a mode in which five protrusions (240) are formed evenly in the vicinity of the outer periphery of the top surface part (210). In general, the petri dish (300) is stored by inverting the top and bottom after filling the culture medium. Therefore, when the protrusion (240) is formed on the top surface part (210) of the lid part (200), it functions as a foot part at the time of storage, reduces friction with the mounting table, and efficiently avoids peeling of the culture medium. be able to. Therefore, the height (h4) of the protrusion (240) is 0.2 to 3 mm, more preferably 0.5 to 2 mm, like the foot (60).

  The said protrusion part (240) may consist of a discontinuous protrusion divided | segmented into plurality similarly to the leg part (60) formed in the above-mentioned petri dish main body (100). Further, it may be arranged on the top surface portion (210) in a laterally or vertically asymmetric manner. Distortion during use and storage can be avoided more efficiently.

Furthermore, it is preferable that the protrusion (240) is also formed with a circular arc at the corner. If the top is an arc, the friction with the mounting table can be further reduced.

FIG. 24 shows a partially enlarged view of the vicinity of the line BB in FIG. FIG. 24 shows a mode in which a gap is formed between the protrusion (240) and the protrusion (240). In the present invention, corners such as the foot (60) are formed by arcs to reduce friction. However, when the petri dish for preparing the fractionation medium is laminated, the laminate collapses with slight vibration. There is. However, if the protrusion (240) is formed on the outer periphery of the upper surface of the lid (200), the protrusion (240) becomes a barrier, and such sliding can be stopped.

(Iii) Rib In the present invention, a rib (235a) disposed in parallel with the bottom surface of the edge height portion (230) is formed inside the edge height portion (230) of the lid portion (200). Also good. Furthermore, a rib (235b) formed on the side wall of the rib (235a) may be formed. FIG. 25 shows a partially enlarged view of the vicinity of the line CC in FIG.

  The height (h5) of the rib (235a) is 0.1 to 1.0 mm. The rib (235a) may be formed in any of the edge height portions (230), and the number thereof is preferably 3 to 20. Moreover, the rib (235b) should just be formed in a part of said rib (235a), and the size is not ask | required.

FIG. 26 shows a cross section taken along line BB in FIG. In addition, a broken line is the petri dish main body (100) at the time of mounting | wearing with a cover part (200). When the rib (235a) is not present, the upper end of the side wall (20) of the petri dish body (100) is in contact with the entire inner surface of the edge height portion (230), but when the rib (235a) is formed, Since the upper end of the side wall (20) of the petri dish body (100) and the rib (235a) are in contact with each other, the occurrence of scratches due to the contact between the lid (200) and the petri dish body (100) is suppressed and air permeability is ensured. By doing so, generation | occurrence | production of the water droplet after filling a culture medium to a bottom face part (10) can be suppressed. Furthermore, the contact area can be reduced, and even when the lid portion (200) vibrates, the amount of transmission of the impact can be reduced, and peeling of the culture medium can be prevented. Similarly, when the rib (235b) is formed, the contact area between the outside of the side wall (20) of the petri dish body (100) and the side wall (220) of the lid (200) is reduced, and similarly to the rib (235a). In addition, the effect of preventing the generation of scratches due to the contact between the lid (200) and the petri dish body (100) and the effect of suppressing water droplets by ensuring air permeability are exhibited. Furthermore, by reducing the contact area, even when the petri dish body (100) or the lid (200) vibrates, the amount of shock transmitted can be further reduced, and the medium can be prevented from peeling off.

(3) Petri dish for preparing a fractionation medium The petri dish body for preparing a fractionation medium (300) of the present invention can be prepared by attaching a lid (200) to the petri dish body for preparing a fractionation medium (100). A transparent petri dish can be produced by using polystyrene or the like. In addition, although the said petri dish main body (100) illustrated in the aspect by which a circular bottom face part (10) is divided | segmented into two small fractions ((alpha), (beta)) by the partition (30), this invention is limited to this. If the side wall (20) is disposed on the outer periphery of the bottom surface portion (10) and the bottom surface portion (10) is divided into two or more by the partition wall (30), the shape of the bottom surface portion (10) May be a square such as a triangle or a square, or the like.

(4) Fractionation medium A fractionation medium can be prepared by filling the petri dish body (100) for preparing a fractionation medium of the present invention with a medium.

As shown in FIG. 27, the medium to be filled may be the same as the upper end of the partition wall (30) or higher than the partition wall (30). When the culture medium is filled so that the partition wall (30) does not protrude, the tip of the platinum ear does not come into contact with the partition wall during the inoculation operation, and the inoculation operation can be performed easily.

(5) Method for Producing Fractionation Medium Making Petri dish The fractionation medium (300) of the present invention can be produced by subjecting the petri dish body (100) and the lid part (200) to injection molding, die cutting and the like, respectively.

Examples of members constituting the petri dish for preparing a fraction medium (300) include polyolefin resins such as polypropylene, polyamide resins, polystyrene, polyethylene terephthalate, polycarbonate, ABS, AS, and the like. In addition, it is preferable that the resin injection port in the case of injection molding is a center part of a bottom face part (10) or a top surface part (210).

  ADVANTAGE OF THE INVENTION According to this invention, the petri dish for fractionation medium preparation in which peeling of the culture medium was suppressed efficiently can be provided, and is useful.

10 ... bottom surface of petri dish body for preparing fractionated medium,
20 ... Side wall of the petri dish body for preparing the fractionation medium,
30 ... partition wall of petri dish body for preparing fractionated medium,
40: a recess formed in the bottom surface of the petri dish body for preparing the fractionation medium,
50 ... a convex portion formed on the bottom surface of the petri dish body for preparing the fractionation medium,
60 ... feet formed on the bottom of the petri dish body for preparing the fractionation medium,
100 ... Petri dish body for preparing the fractionation medium,
200 ... lid,
210 ... the top surface of the lid,
220 ... side wall of the lid,
230 ... the height of the edge of the lid,
235 ... a rib formed on the inner side of the rim,
240 ... a protrusion formed on the upper surface of the lid,
300 ... Petri dish for preparing the fractionation medium of the present invention

Claims (4)

A bottom surface portion, a side wall disposed on the outer periphery of the bottom surface portion, and a partition wall that divides the bottom surface portion into a plurality of small fractions and that is connected to the side wall,
The bottom portion is formed with a concave portion provided in the vicinity of the partition wall and a convex portion provided in the vicinity of the side wall,
Discontinuous feet are formed near the outer periphery of the bottom surface,
A petri dish body for preparing a fractional culture medium, wherein the convex portions and the foot portions are arranged so as to fill the gaps between the convex portions and between the foot portions.   The petri dish body for preparing a fractional culture medium according to claim 1, wherein the partition wall disposed in the petri dish body has a cross-sectional view and an arch shape.   A petri dish for preparing a fractionation medium comprising the petri dish body for preparing a fractionation medium according to claim 1 and a lid. The lid portion includes a top surface portion and side walls disposed on the outer periphery of the top surface portion.
The petri dish for preparing a fractionation medium according to claim 3, wherein an edge height part that can be fitted to an upper end of a side wall of the petri dish body is formed on an inner surface of the top surface part.

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