JP5698586B2 - Cosmetics - Google Patents

Description

  The present invention relates to cosmetics having excellent skin beautification and whitening effects and high safety.

  Skin aging is caused by internal factors such as inactivation of cell growth / differentiation associated with aging, decreased hormone secretion, quantitative decrease of extracellular matrix components, and cells caused by active oxygen induced by sunlight (ultraviolet rays). -Phenomenon caused by complex intertwining with external factors such as tissue damage or inflammation. Among them, internal factors include decreased activity or proliferation ability of dermal fibroblasts, decreased extracellular matrix components such as collagen, elastin, and hyaluronic acid, and inactivation of epidermal basal cells. Due to these internal factors, the skin stratum corneum barrier function, sebum secretion function, and water retention function decrease, and as a result, symptoms such as wrinkles, sagging, and rough skin appear as morphological and physiological changes in the skin.

  In addition, active oxygen, which is an external factor of skin aging, not only directly damages skin cells, but also denatures or crosslinks the collagen of the extracellular matrix component, resulting in wrinkle formation and reduced skin elasticity. Causes various damages to the skin, such as causing abnormal deposition of melanin pigment and causing spots and freckles.

  In order to prevent this skin aging and keep the skin healthy and youthful, the use of various active ingredients has been proposed in the past, and cosmetics containing these skin-beautifying and whitening ingredients have been put on the market. For example, vitamin C, vitamin E, superoxide dismutase (hereinafter abbreviated as SOD), an antioxidant such as catalase; an anti-inflammatory agent such as glycyrrhizic acid; various ultraviolet absorbers; α-hydroxycarboxylic acid, placenta extract Cell activation components such as γ-amino-β-hydroxybutyric acid; extracellular matrix components such as collagen, elastin and hyaluronic acid; and humectants such as urea. Further, arbutin, kojic acid, and the like are known as substances that suppress the occurrence of pigmentation such as skin spots and buckwheat, and are widely used as active ingredients of whitening agents.

  However, these conventional skin-beautifying agents and whitening agents have problems in terms of safety to the skin and the like and effectiveness when actually applied to the skin. In addition, skin-beautifying agents and whitening agents derived from various natural ingredients have been proposed, but the skin-beautifying and whitening effects of these agents are safe for the skin when viewed as cosmetic ingredients, There are problems in terms of effectiveness when actually applied to the skin. Accordingly, there is a need for cosmetics containing cosmetic ingredients with improved such points.

  In view of the problems of the prior art, the present inventors have intensively studied to find new skin-beautifying and whitening ingredients derived from natural products from the viewpoint of skin safety. As a result, the cocoon extract of the lily family Lily genus plant has an activity suppressing action and an antioxidant action of elastase which is an elastin degrading enzyme, and further an tyrosinase activity suppressing action, and the coconut extract is blended. Thus, the present inventors have found that it is possible to provide a cosmetic having excellent skin beautification and whitening effects and having excellent skin safety, and have completed the present invention.

Conventionally, it has been known that flowers and leaves of lily family plants have effects of moisturizing and improving rough skin, and are incorporated into cosmetics as an anti-aging active ingredient (Patent Documents 1 and 2). It is also known that a lily family lily plant flower extract has a melanin production inhibitory effect and is incorporated into cosmetics as a whitening active ingredient (Patent Document 3). However, there has been no report that an extract of camellia of the lily family Lily genus plant has an excellent anti-aging effect or whitening effect.
In view of the above, as a result of intensive studies conducted by the present inventors, the extract of the lily family, particularly the bud, is superior to the extract of other parts (flowers, leaves, stems). The present inventors have newly found out that they have an elastase activity inhibitory action and an antioxidant action, and also have a whitening effect based on a tyrosinase activity inhibitory action, thereby completing the present invention.
Furthermore, the present inventor has also proposed a protein saccharification reaction (Maillard reaction), which has recently attracted attention as a mechanism of skin aging, and an advanced glycation end produced by the reaction. The present invention has been completed by newly discovering that the accumulation of the final product of protein glycation reaction called advanced glycation end products (AGEs) is also suppressed.

JP 2003-012489 A JP 2010-018594 A JP 2003-238343 A

The present invention is a cosmetic comprising an extract of a cocoon of a plant belonging to the genus Liliumaceae (Liliumceae).
As the plant belonging to the lily family, the cultivar Casablanca is particularly preferable.

  The cosmetic composition of the present invention, which is obtained by blending a cocoon extract of a plant belonging to the lily family Lily genus, has a strong elastase activity inhibitory action and an antioxidant action exhibited by an extract of cocoon contained as an active ingredient. Gloss can be improved to prevent and improve skin wrinkles and sagging. Furthermore, pigmentation such as spots and buckwheat can be prevented and improved by an antioxidant action and a tyrosinase activity inhibitory action. In addition, since the extract is derived from a natural product, there is little irritation to the skin and it is excellent in safety.

Hereinafter, the present invention will be described in detail.
The species of the plant belonging to the genus Lilium (Liliaceae) used in the present invention are not particularly limited, and examples thereof include Lily auratum Lindl., Lilium lancifolium Thunb., Lilium brownii FEBr.), Lily nobilissimum Makino, Lily candidum L., Lilium concolor Salisb., Lilium formosanum Wallace, Lilium leichtlinii Hook.f.var.tigrinum (Regel) Nichols.), Lilium longiflorum
Thunb.), Lily lily (Lilium
maculatumThunb.), lily medeoloides A.Gray, lilium pumilium, legal lily (Lilium regale Wils.), lily rubellum Baker, lily speciosum Thunb., lily tenuifolium Fisch.), Or varieties and hybrids of these lily plants can also be used. There are many cultivars known in the genus Lily, such as Asiantic Hybrid, Longiflorum Hybrid, Marthagon Hybrid, Trumpet Hybrid, Oriental Hybrid, and the lily of these cultivars is used. However, in the present invention, it is preferable to use a Casablanca known as an Oriental hybrid because of its effect and good yield. Note that Casablanca is a variety cultivated by crossing the above-mentioned Yamayuri, Kanoko Yuri, Tamoto Yuri and the like.

  As the buds of the lily family Lily genus plants, those of any period can be used, from immature to those close to flowering, but those having a major axis in the range of 1 cm to 10 cm are preferably used. .

  For the preparation of the extract, if necessary, the lily family lily genus plants are washed with water in advance to remove foreign substances, and then, as it is or dried, and then chopped or pulverized as necessary, soaking method or supercritical extraction It can be performed by contacting with an extraction solvent according to a conventional method such as a method.

  As an extraction solvent, water; lower alcohols such as methanol, ethanol, propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin; ethyl acetate, butyl acetate, methyl propionate, etc. Esters; Ketones such as acetone and methyl ethyl ketone; Ethers such as ethyl ether and isopropyl ether; Hydrocarbon solvents such as n-hexane, toluene and chloroform, and the like. These may be used alone or in combination of two or more. Used.

  Among these extraction solvents, from the viewpoint of the elastase activity inhibitory action, antioxidant action, and tyrosinase activity inhibitory action of the extract obtained, and also from the viewpoint that it can be widely applied to cosmetics, in the present invention. Hydrophilic solvents such as water, lower alcohols or polyhydric alcohols are preferred. Preferable examples in the case of using this hydrophilic solvent include, for example, water or lower alcohols (especially ethanol) used alone, a mixed solvent of water and lower alcohols (especially ethanol), or water and polyhydric alcohols ( In particular, use of a mixed solvent with 1,3-butylene glycol or propylene glycol) and the like are exemplified, and water alone is most preferable.

  The mixing ratio in the case of using a mixed solvent is, for example, 1: 1 to 25: 1 in a volume ratio (hereinafter the same) if the mixed solvent is water and ethanol, and 1: 1 if the mixed solvent is water and glycerin. If it is -20: 1 and it is a mixed solvent of water and 1,3-butylene glycol, it is preferable to set it as the range of 1: 1 to 20: 1.

  In addition, the weight ratio of the cocoon (dried) of the plant belonging to the genus Lily family and the extraction solvent is preferably in the range of 1: 1 to 1:50, more preferably in the range of 1: 5 to 1:30. .

  In preparing the extract, the pH is not particularly limited, but it is generally preferably in the range of 4-8. In this sense, if necessary, the extraction solvent is blended with an alkaline adjusting agent such as sodium hydroxide, sodium carbonate or potassium hydroxide, or an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid. You may adjust so that it may become pH.

  Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent to be used. For example, when water is used as the extraction solvent, the extraction temperature is preferably in the range of 4 to 90 ° C., and the extraction time. Is preferably in the range of 1 to 6 hours.

  In general, the extract obtained under the above conditions may be adjusted to pH 4 to 8 and used as it is as a cosmetic compounding agent or as a desired concentration by vacuum concentration or the like.

  In addition, the extract used in the present invention may be subjected to treatments such as solvent fractionation, column purification, activated carbon treatment, deodorization treatment, and decolorization treatment as long as the effects of the present invention are not impaired.

  The extract of the present invention prepared as described above has remarkable elastase activity inhibitory action, antioxidant action, and tyrosinase activity inhibitory action as shown in the test examples described later, and has little irritation to the skin and is biologically safe. Because it is also superior in nature, cosmetics formulated with this extract prevent the generation of wrinkles, sagging, stains, freckles or improve their symptoms, and keep the skin youthful and healthy. I can.

  Examples of the cosmetics containing the lily family lily genus extract of the present invention include basic cosmetics such as emulsions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, makeup press powders, cheeks , Makeup cosmetics such as white powder, cleansing cosmetics such as facial cleansers, body shampoos, and soaps, and bath agents, but of course are not limited thereto.

  In the cosmetics of the present invention, the amount of the extract of the cocoon of the plant belonging to the genus Lily family is generally 0.002 to 1.0% by weight, preferably in the case of basic cosmetics, as the solid content of the extract, In the range of 0.02 to 0.2% by weight, in the case of makeup cosmetics, generally 0.002 to 1.0% by weight, preferably in the range of 0.02 to 0.2% by weight, and cleaning cosmetics Is generally in the range of 0.002 to 10.0% by weight, preferably 0.02 to 7.0% by weight.

  The cosmetics of the present invention include, in addition to extracts of plant buds belonging to the genus Lily, an essential component, components that are usually used in cosmetics, such as oily components, surfactants (synthetic and natural products). , Moisturizers, thickeners, preservatives / bactericides, powder components, ultraviolet absorbers, antioxidants, pigments, fragrances and the like can be appropriately blended as necessary. Moreover, as long as the effectiveness and the feature of the extract of the cocoon of the plant which belongs to the lily family Lily genus of this invention are not impaired, it may mix | blend with cosmetics in combination with another physiologically active ingredient.

  Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Oils derived from plants such as macadamia nut oil and plant-derived squalane; Fats derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, me Butyl phosphate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, Quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.
In addition, as emulsifiers or emulsifiers, stevia derivatives such as enzyme-treated stevia, lecithin and its derivatives, lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, legumes, miscellaneous cereals, etc.), juteiro (Rhamnaceae zizyphus joazeiro) An extract or the like can also be blended.

  Examples of humectants include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidonecarboxylate, and sugars such as trehalose, lactic acid bacteria fermented rice, and mucopolysaccharides. (For example, hyaluronic acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, beech extract Products, seafood-derived collagen and derivatives thereof, various amino acids and derivatives thereof.

  Examples of the thickener include brown algae, green algae or red algae-derived components such as alginic acid, agar, carrageenan and fucoidan; beech extract; polysaccharides such as pectin, locust bean gum and aloe polysaccharide; xanthan gum, tragacanth gum, guar gum and the like Gums; Cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; Synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer and acrylic acid / methacrylic acid copolymer; Hyaluronic acid and its derivatives; Polyglutamic acid and its derivatives Is mentioned.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Luconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), 1,2-pentanediol, various essential oils, bark dry matter, and the like.

  Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. System powders, powders of cereals (rice, wheat, corn, millet, etc.), powders of beans (soybeans, red beans, etc.).

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

  Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives, beech extract, rice extract and the like.

Examples of physiologically active ingredients include whitening ingredients such as t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone derivatives, ellagic acid and derivatives thereof, resorcinol derivatives, tranexamic acid and derivatives thereof, 4-methoxy Salicylic acid potassium salt, magnolignan (5,5′-dipropyl-biphenyl-2,2′-diol), 4-HPB (rhodenol, 4- (4-hydroxyphenyl) -4-butanol)), AMP (adenosine monophos) Fate, adenosine monophosphate), placenta extract, nicotinic acid and its derivatives, Sakuhakuhi extract, yukinoshita extract, rice bran extract, rice bran extract hydrolyzate, lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals ( Wheat, beans, cereals), white coconut extract, white coconut hydrolyzed extract, Rasakishikibu extract, lotus seed fermentation product, peony extract, party ginseng extract, Coix fermented product, sake lees fermentation product, Pandanusu Amari Rifo Cruccuris (Pandanus
amaryllifolius Roxb.) extract, Arcangelicia flava
Merrilli) extract, chamomile extract (trade name: chamomile ET), seaweed extract such as kombu, seaweed extract such as sea cucumber, linoleic acid and its derivatives or processed products (eg liposomal linoleic acid), 2 , 5-dihydroxybenzoic acid derivatives and the like, and as skin aging preventing / beautifying components, collagen derived from animals or fish and derivatives thereof, elastin and derivatives thereof, nicotinic acid and derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salts, etc.) ), T-cycloamino acid derivatives, vitamin A and derivatives thereof, vitamin E and derivatives thereof, allantoin, α-hydroxy acids, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, barley extract, Chamomile extract, carrot extract, aloe extract How herbal extract, rice extract hydrolyzate, rice bran extract hydrolyzate, rice fermentation extract, Mitsuishikonbu extract, Ulva pertusa extract, seaweed extracts such as eelgrass, mulberry bark extract, Juazeiro (Rhamnaceae zizyphus
joazeiro) extract.

  Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid), L-ascorbic acid-5-glucoside (5-O-α) -D-glucopyranosyl-L-ascorbine Acid) ascorbic acid sugar derivatives, acylated 6-positions of these ascorbic acid sugar derivatives (acyl groups are hexanoyl, octanoyl, decanoyl, etc.), L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetra L-ascorbic acid tetrafatty acid esters such as lauric acid ester, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium and the like are hydroquinone derivatives such as arbutin (hydroquinone). -Β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside) and the like, and as resorcinol derivatives, for example, 4-n-butylresorcinol, 4-isoamylresorcinol and the like are 2,5-dihydroxybenzoic acid. Derivatives and For example, 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid, etc., and nicotinic acid derivatives include, for example, nicotinic acid amide, nicotinic acid benzyl, etc. However, examples of the vitamin E derivative include vitamin E nicotinate and vitamin E linoleate, and examples of the α-hydroxy acid include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

  Next, the present invention will be described more specifically with reference to production examples, examples (formulation examples), and test examples, but the present invention is not limited thereto. In the following, all parts are parts by weight, and all percentages are% by weight.

Production Example 1 Preparation of cocoa extract of Casablanca (1)
Casablanca pods of the Lily family Lily genus plant are dried and pulverized, 100 g of purified water is mixed with 10 g of the pulverized product, extracted at 40 ° C. for 2 hours, and then filtered to obtain 75 g of brown transparent Casablanca pod extract. (Solid content concentration 1.9%).

Production Example 2 Preparation of Casablanca cocoon extract (2)
Casablanca cocoons of the lily family Lily genus plant are dried and pulverized, mixed with 20 g of pulverized water and 400 g of purified water, extracted at 80 ° C. for 2 hours and filtered to obtain 340 g of brown transparent Casablanca cocoon extract. (Solid content concentration 1.8%).

Production Example 3 Preparation of Casablanca cocoon extract (3)
Casablanca cocoons of the lily family Lily genus plant are dried and pulverized, mixed with 50 g of purified water and 500 g of purified water, extracted at 80 ° C. for 2 hours and filtered to obtain 430 g of brown transparent Casablanca cocoon extract. (Solid content concentration 3.5%).

Production Example 4 Preparation of Casablanca cocoon extract (4)
Casablanca cocoons of the Lily family Lily genus plant are dried and pulverized, and 10 g of the pulverized product is mixed with 100 g of a 7: 3 (weight ratio) mixture of purified water and 1,3-butylene glycol, and extracted at 80 ° C. for 2 hours. After filtration, 70 g of a brown transparent Casablanca cocoon extract was obtained (solid content concentration 1.4%).

Production Example 5 Preparation of Casablanca cocoon extract (5)
Casablanca cocoons of the lily family Lily genus plant are dried and pulverized, mixed with 70 g of purified water and 700 g of purified water, extracted at 80 ° C. for 2 hours and filtered, and the filtrate is treated with activated carbon. 440 g of a brown transparent Casablanca cocoon extract was obtained (solid content concentration 3.2%).

Production Example 6 Preparation of dried Casablanca cocoons (6)
300 g of Casablanca cocoon extract obtained in the same manner as in Production Example 5 was freeze-dried and then pulverized to obtain 10 g of a brown powder.

Comparative Production Example 1 Preparation of Casablanca flower extract (1)
Casablanca flowers (lily flowers) of the lily family Lily genus plant are dried and pulverized, 70 g of purified water is mixed with 7 g of the pulverized product, extracted at 80 ° C. for 2 hours and filtered, and the filtrate is filtered with activated carbon. After processing, 37 g of a light yellowish-brown transparent Casablanca flower extract was obtained (solid content concentration 4.7%).

Comparative Production Example 2 Preparation of Casablanca leaf extract (1)
Casablanca leaves of the lily family Lily genus plant are dried and ground, mixed with 5 g of purified water and 50 g of purified water, extracted at 80 ° C. for 2 hours, filtered, and the filtrate is treated with activated carbon. 35 g of a brown transparent Casablanca leaf extract was obtained (solid content concentration 4.7%).

Comparative Production Example 3 Preparation of Casablanca stem extract (1)
Casablanca stalk of Lily family Lily genus plant is dried and pulverized, 70 g of purified water is mixed with 7 g of the pulverized product, extracted for 2 hours at 80 ° C., filtered, and the filtrate is treated with activated carbon. A brown transparent Casablanca stalk extract (39 g) was obtained (solid content concentration 4.7%).

Example 1. Cream [Component A] Liquid paraffin 5.0
Hexalan (Note 1) 4.0
Paraffin 5.0
Glyceryl monostearate 2.0
Polyoxyethylene (20) sorbitan monostearate 6.0
Butylparaben 0.1
(Note 1) Technoble Co., Ltd. glyceryl trioctanoate
[B component]
Extract 5 of Production Example 5.0
Glycerin 5.0
Carboxymethyl monostearate 0.1
Moiston C (Note 2) 1.0
Amount of purified water 100 parts (Note 2) NMF component manufactured by Technoble Co., Ltd.
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After this was cooled to 50 ° C., component C was added and further stirred and mixed to obtain a cream.

Example 2 Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Extract 5 of Production Example 5.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Example 3 Lotion [component A] part Extraction liquid of Production Example 5 5.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Perfume
Potassium hydroxide appropriate amount Purified water Amount that makes 100 parts in total
The above ingredients were mixed to obtain a lotion.

Example 4 Lotion [A component] part olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
[B component]
Extract solution of Production Example 5 5.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Potassium hydroxide appropriate amount Purified water Amount that makes 100 parts in total
[C component]
Perfume
After each component A and component B was heated to 80 ° C. or higher, the component B was added to the component A and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.

Example 5 FIG. Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Extract solution of Production Example 5 5.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Production Example 6 Emulsion Other than using 2.0 parts of L-ascorbic acid-2-phosphate magnesium in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the component B of Example 5. Obtained an emulsion in the same manner as in Example.

Example 7 Emulsion In addition to using 2.0 parts of L-ascorbic acid-2-phosphate and 2.0 parts of L-ascorbic acid-2-phosphate in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the component B of Example 5 Obtained an emulsion in the same manner as in Example 5.

Example 8 FIG. Emulsion In the component B of Example 5, the emulsion was prepared in the same manner as in Example 5 except that 2.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide. Obtained.

Example 9 Latex In the component B of Example 5, in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide, a rice bran extract hydrolyzate (trade name “Grace Snow” manufactured by Technoble Co., Ltd.) An emulsion was obtained in the same manner as in Example 5 except that 5.0 parts of * Snow * HP ", solid concentration 3.5%) were used.

Example 10 Emulsion White coconut extract (trade name “Sinablanca-WH” manufactured by Technoble Co., Ltd.) instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide in the B component of Example 5 A solid emulsion was obtained in the same manner as in Example 5 except that 5.0 parts of a solid content concentration of 1.0% was used.

Example 11 Emulsion Example 5 in the component B of Example 5, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide were used instead of 1.0 part of γ-amino-β-hydroxybutyric acid In the same manner as in No. 5, an emulsion was obtained.

Example 12 Liquid foundation [component A] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[Component B] Part Extract solution of Production Example 5 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount that makes the total amount 100 parts [Component C] Part Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount The components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.

Example 13 Cream foundation [component A] part Stearic acid 5.0
Cetanol 2.0
Glyceryl monostearate 3.0
Liquid paraffin 5.0
Squalane 3.0
Isopropyl myristate 8.0
Polyoxyethylene (20) glyceryl monostearate 2.0
Propylparaben 0.1
[Component B] Part Extract solution of Production Example 5 5.0
Sorbitol 3.0
1,3-butylene glycol 5.0
Triethanolamine 1.5
Methylparaben 0.1
Purified water Amount that makes the total amount 100 parts [Component C] Part Titanium oxide 8.0
Talc 2.0
Kaolin 5.0
Bentonite 1.0
Coloring pigment appropriate amount [Component D]
Fragrance 0.3
Component C was mixed and pulverized with a pulverizer. The component B was mixed, and the pulverized component C was added thereto and uniformly dispersed in a colloid mill. The components A and B and C dispersed uniformly were each heated to 80 ° C., and then the components A were added to the components B and C while stirring, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, D component was added and stirred and mixed, and also it cooled to 30 degrees C or less, stirring, and obtained the cream foundation.

Example 15. Body shampoo [A component]
Part Sodium N-lauroylmethylalanine 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[Component B] Part Extract solution of Production Example 5 5.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a body shampoo It was.

Test Example 1 Elastase Activity Inhibition Test The elastase activity inhibitory action of the extract of Production Example 5 and the extract of Comparative Production Examples 1 to 3 was examined.
[Test method]
<Prepared solution>
(1) Eagle MEM containing 10% NCS
(2) Cell lysate (3) Substrate solution <Preparation method>
(1) 1 L of distilled water was added to 9.4 g of Eagle MEM medium (manufactured by Nissui Pharmaceutical Co., Ltd.), and the final concentration was 10% by volume NCS (calf serum), 1.4% by weight sodium bicarbonate, 0.03% by weight, respectively. Prepare by adding% glutamic acid.
(2) 1 mM PMSF, 1% Triton X-100 at 100 mM
Dissolved in Tris-HCl (pH 8.0).
(3) Succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilide (Suc-Ala-Ala-Ala-pNA) was dissolved in 100 mM Tris-HCl (pH 8.0).
<Measurement method>
The extract solutions of Production Example 5 and Comparative Production Examples 1 to 3 were diluted to 2.5% or 5.0% (as solutions), respectively, to prepare sample solutions. In addition, 5% PBS (-) solution was used as a sample non-addition (control).
Normal human skin-derived fibroblasts (NB1RGB) were prepared to 1 × 10 ⁵ cells / mL with Eagle's MEM containing 10% by volume of NCS, seeded at 100 μL each in a 96-well microplate, and under 5% carbon dioxide gas and saturated water vapor. And cultured at 37 ° C. After 48 hours, the culture solution was removed, and the cells were washed once with PBS (−). Then, 50 μL of the cell lysate was added and left at room temperature for 10 minutes to lyse the cells, and this was used as an enzyme solution. It was. To a 96-well microplate, 50 μL of each sample solution was added to 50 μL of the enzyme solution, and further 100 μL of the substrate solution was added, followed by reaction at 37 ° C. in the dark for 2 hours. As a blank, a cell lysate was used for the test instead of the enzyme solution. Thereafter, the absorbance after the reaction at 405 nm was measured with a plate reader. The elastase activity rate was calculated from the measured value of each absorbance using the following formula (1). In addition, the same test was done using 1 mM EDTA as a sample solution as a positive control.
Formula (1): Fibroblast elastase activity rate (%) = (Es-Esb) / (Ec-Ecb) × 100
Ec: Absorbance of control (PBS (-)) test group Ecb: Absorbance of control (PBS (-)) blank group Es; Absorbance of test group to which sample solution was added Esb; Absorbance of blank group to which sample solution was added

[result]
The results of Test Example 1 are shown in Table 1.
[Table 1]
From the results in Table 1, it can be seen that the extract of the cocoon of the plant belonging to the genus Lily family (Casablanca) significantly suppresses the elastase activity as compared with the extract of other parts (flowers, leaves, stems). Admitted. Moreover, the elastase activity suppression effect which was excellent especially was recognized also compared with 1 mM EDTA which is a positive control.

Test Example 2 Active oxygen scavenging ability (SOD-like activity) test Each of the SOD-like activities of the extract of Production Example 5 and the extracts of Comparative Production Examples 1 to 3 was examined.
[Test method]
0.15 mL of 1M Tris-HCl buffer solution, 0.30 mL of 1 mM ethylenediaminetetraacetic acid / disodium salt solution, 0.30 mL of 1 mM xanthine solution, 0.20 mL of 0.75 mM nitrobull-tetrazolium solution, 0.10 mL of the extract solution of Production Example 5 And 1.90 mL of purified water were mixed to prepare a test solution. For comparison, a mixed solution having the same composition as the above test solution was prepared except that 0.10 mL of each of the extract solutions of Comparative Production Examples 1 to 3 was used instead of the extract solution of Production Example 5 in the test solution. In addition, in the test solution, a mixed solution (no sample added) having the same composition as the above test solution was prepared except that 2.00 mL of purified water was used instead of 0.10 mL of the extract of Production Example 5 and 1.90 mL of purified water. did. Further, in the test solution, a mixed solution having the same composition as the above test solution except that 0.10 mL of 0.875 or 8.75 Unit / mL superoxide dismutase solution is used instead of 0.10 mL of the extract of Production Example 5 ( Positive control) was prepared.
After incubating each of the above test solution or the sample-free mixed solution at 37 ° C., 0.05 mL of 1 Unit / mL xanthine oxidase solution was added thereto, and after a certain period of time (5 minutes), each test solution at 560 nm was added. Absorbance (an index of the amount of superoxide anion in the test solution) was measured. As a result, the absorbance of each test solution or the mixed solution of the positive control (superoxide dismutase) when the absorbance of the mixed solution with no sample added (purified water) was defined as 100% was expressed in%.

[result]
The results are shown in Table 2.
[Table 2]
As is clear from the results in Table 2, the extract of the cocoon of the plant belonging to the genus Lily of the present invention (Casablanca) has a higher SOD compared to the extract of other parts (flowers, leaves, stems). It became clear that it had like-like activity. Moreover, it became clear that it has SOD like activity equal to or more than the positive control superoxide dismutase.
In addition, in the result of the xanthine-xanthine oxidase reaction test performed in parallel with this test, the extract of Production Example 5 does not suppress the xanthine-xanthine oxidase reaction, which is the first-stage reaction in this evaluation test. confirmed.

Test Example 3 Intracellular tyrosinase activity inhibitory action The extract of Production Example 5 and the extracts of Comparative Production Examples 1 to 3 were examined for their respective intracellular tyrosinase activity inhibitory actions.
[Test method]
Cultured B16 mouse melanoma cells were seeded at 8 × 10 ³ cells / well in a 96-well microplate and placed in Eagle's minimum essential medium (MEM) containing 10% calf serum (FBS) under conditions of 37 ° C. and 5% CO ₂ . After pre-culturing for 1 day, the extract of Production Example 5 and the extracts of Comparative Production Examples 1 to 3 were respectively 2.5% and 5.0% in concentration (as a solution) with Eagle MEM containing 10% FBS. The solution was diluted with the diluted solution and cultured for 2 days under the same conditions.
Next, after removing the culture solution and adding 0.3 mg / mL MTT solution or adding a surfactant (Triton X-100) and 5 mM L-dopa solution and reacting at 37 ° C. The MTT value was measured at a wavelength of 570-630 nm and the dopa value was measured at a wavelength of 490 nm using a microplate reader (Model 450, manufactured by Bio-Rad).
For comparison, the same test was performed with PBS (-) and with kojic acid (positive control).

[result]
The results are shown in Table 3.
[Table 3]
As shown in Table 3, the cocoon extract of the plant belonging to the genus Lily family (Casablanca) of the present invention is compared with the extract of other parts (flowers, leaves, stems), particularly at a low concentration of 2.5%. Thus, it was clarified that the cultured B16 mouse melanoma cells have a stronger tyrosinase activity-inhibiting action without reducing or inhibiting the activity.

Test Example 4 Protein Saccharification Inhibitory Effect Test Furthermore, in this test, the generation inhibitory effect of AGE, that is, the protein saccharification inhibitory effect, was evaluated by the generation and color development of BSA (bovine serum albumin) fluorescence via glucose. First, 40 μL of the extract of Production Example 5, 40 μL of 50 mg / mL BSA aqueous solution, 40 μL of 2.5 M glucose aqueous solution, and 80 μL of PBS (−) solution were mixed and stirred to prepare a sample solution. The sample solution was prepared so that the final concentrations of the extract of Production Example 5 were 1.0%, 2.0%, and 5.0%, respectively. Next, the sample solution is allowed to stand at 50 ° C. for 7 days, and after 7 days, for each sample solution, fluorescence generation (excitation: 355 nm, emission: 460 nm: fluorescence microplate reader (Fluoroscan Ascent, manufactured by Thermo Fisher Scientific)) And absorbance (wavelength 405 nm: microplate reader (Model 450, manufactured by Bio-Rad)). In addition, the same operation was performed in the case of no sample addition (control) using purified water instead of the extract of Production Example 5, and the fluorescence measurement value obtained here and the fluorescence measurement value of each sample solution with respect to the absorbance And the relative value (%) of the absorbance was determined as the protein saccharification rate (%). Furthermore, the same test was performed when 1 mM aminoguanidine (AG) was added as a positive control instead of the extract of Production Example 5.

The results of Test Example 4 are shown in Tables 4 and 5. Table 4 shows the protein saccharification rate calculated based on the fluorescence measurement result, and Table 5 shows the protein saccharification rate calculated based on the absorbance measurement result.
[Table 4]

[Table 5]

As shown in Tables 4 and 5, the sputum extract of a plant belonging to the genus Lily of the present invention (Casablanca) has suppressed the generation of fluorescence and color development of BSA via glucose, that is, the present invention It was confirmed that the extract of coconut cocoon has a remarkable protein glycation-inhibiting effect. In addition, since AG which is a positive control also suppressed fluorescence generation, it was confirmed that this test system was normal.

Claims (2)

  A cosmetic comprising an extract of cocoon of a plant belonging to the genus Liliumaceae and Lilium as an active ingredient. The cosmetic according to claim 1, wherein the lily family Lily plant is Casablanca.