JP5784416B2 - How to collect fungi - Google Patents

Description

  The present invention relates to a recovery method for recovering fungi cultured from a microorganism culture.

Today, various microbial spores and conidia are produced industrially for use as the raw materials for biological pesticides and as seeds for brewing and brewing.
In this industrial production method of microorganisms, when microorganisms are cultured, most of the dry weight is a medium component, and the weight of spores and conidia contained in the culture is very small. Moreover, when processing the spore and conidia in a culture into a product, impurities, such as a culture medium component, may reduce quality. For this reason, in the method for producing spores and conidia, it is necessary to remove unnecessary components such as medium components and selectively recover spores and conidia.
Therefore, as a method for recovering fungi such as spores and conidia from the culture, a wet recovery method of filtering with a screen mesh or filter cloth (see, for example, Patent Documents 5 to 8), or a dry recovery method of classifying vibration or airflow ( For example, see Patent Documents 1 to 4).

In the wet recovery method of Patent Document 1, a culture after solid-cultivating filamentous fungi is dispersed in a medium containing water or a buffer solution or physiological saline, and the resulting suspension is cultured. The product residue and spore dispersion are separated using filter paper, filter cloth, sieve, or the like. Thereafter, the spore dispersion is mixed with an excipient and granulated to collect spores.
However, in the method described in Patent Document 1, since the amount is increased with a medium such as water, the amount of processing increases, and a large amount of energy is required to remove the medium during granulation, and spores may not be recovered efficiently. In addition, the heat load during granulation may cause spore recurrence, spores dying due to overdrying, and the spores may not be collected efficiently. In addition, when the spores are hydrophobic, the recovery rate may be significantly reduced.

In the wet recovery method described in Patent Document 2, the culture is mixed with a non-aqueous liquid such as hydrocarbon oil, fatty acid ester oil, or silicone oil, and the culture residue is separated from the resulting mixture by sedimentation or filter cloth or wire mesh. The structure which removes by the fraction using this and obtains microbial suspension is taken.
However, in the method described in Patent Document 2, it is difficult to re-separate the spore and the non-aqueous liquid, and there is an inconvenience limited to the product form used as a suspension. Further, during storage of the microbial suspension, the spores may precipitate and agglomerate, which may result in non-uniformity and may not provide stable quality.

The wet recovery method described in Patent Document 3 employs a configuration in which a solid culture is mixed with liquid paraffin or white mineral oil and then squeezed with a cotton cloth to obtain a spore suspension.
Even in the method described in Patent Document 3, as in the method described in Patent Document 2, it is difficult to separate only the suspended spores from liquid paraffin or white mineral oil. There is a risk that the spores may precipitate and aggregate.

On the other hand, the dry recovery method of Patent Document 4 employs a configuration in which spores are recovered from the culture using a dry sieve.
Further, the dry recovery methods of Patent Documents 5 and 6 adopt a configuration in which a solid material on which spores are formed is crushed and sieved.
Furthermore, the dry recovery method of Patent Document 7 also adopts a configuration in which spores are recovered by sieving a culture in which spores are formed.
However, in the method of recovering spores using these sieves, since the spores are recovered by gravity dropping through the sieve, it takes time to recover very light spores, and improvement in recovery efficiency is desired. Furthermore, since the spores are very light, they are likely to be scattered, the recovery rate is lowered, and the working environment for separation and recovery may be deteriorated. Also, due to the aggregation of the spores themselves and the adherence of organic substances such as sugars, proteins, and lipids, which are the medium components, spores may remain on the medium components, adhere to equipment, and clog the screen. There is a risk that work efficiency and recovery efficiency may be reduced. For this reason, in order to improve the detachability from the medium components, there is a method of pre-grinding the culture as described in Patent Document 5, for example. There is a risk that the amount of unrecovered material will increase due to the adhesion.

Further, the dry recovery method of Patent Document 8 employs a configuration in which a conidia is taken out by turbulence and friction between particles by being compressed by a Wiley mill from which a cutting blade is removed.
However, when using a mill using a wheelie mill described in Patent Document 8 and other grinders such as a hammer mill, a power mill, a pin mill, and a jet mill, the heat and physical impact on the spores and conidia are large, and the recovery rate is due to death. May decrease. In addition, the medium component is pulverized by over-pulverization, and the medium component is mixed as an impurity together with the spores and conidia to be collected, so that the ratio of the impurity increases and the quality may be deteriorated.

And the structure which collect | recovers spores, conidia, etc. from a culture using a cyclone is described in the dry-type collection | recovery method of patent document 9. FIG.
However, in the method of classifying airflow, such as using the cyclone of Patent Document 9, since the airflow is weak and cannot be sufficiently recovered, there is a disadvantage that equipment such as a large compressor and piping for generating a large airflow is required. . Moreover, in order to efficiently separate spores, conidia, and the like from the medium components, if the above-described pulverizer is used, the recovery rate may decrease due to death or the ratio of impurities may increase as described above. .

Japanese Patent Laid-Open No. 2003-63917 JP 2010-81832 A JP 2006-124337 A Japanese Patent Laid-Open No. 7-8272 JP 2003-277214 A JP 2009-221132 A Japanese Patent No. 4159688 JP-T 9-506592 Japanese Patent No. 4274750

As described above, in the conventional wet recovery method, the product form is limited, and it is difficult to improve the recovery rate, and there is a possibility that the quality is deteriorated due to coagulation sedimentation.
In addition, conventional dry recovery methods such as a method using a sieve, a method using a pulverizer, and a method for classifying an air flow can improve the recovery rate due to inefficiencies in processing capacity, clogging, etc., and scattering of the lattice. There is a possibility that improvement of working environment and improvement of viable bacteria rate may not be obtained.

  An object of the present invention is to provide a method for collecting fungi that can efficiently and easily collect fungi such as spores and conidia at a high viable cell rate.

The present invention is a recovery method for recovering fungi cultured from a microorganism culture, using a screen mesh through which the fungi can pass and culture residues of the microorganism culture cannot pass, It is characterized in that the microorganism culture is introduced into an air current having a wind speed of 0.3 m / second or more and 0.65 m / second or less in a direction in which fungi pass.
Here, the culture residue of the microorganism culture corresponds to impurities other than fungi for culturing fungi such as medium components.
In this invention, by introducing a microorganism culture into an air current with a wind speed of 0.3 m / second or more and 0.65 m / second or less, fungi such as spores and conidia can be formed in the culture medium due to wind pressure and collision between the microorganism cultures. It is separated from the components and collected through the screen mesh with the air stream.
This prevents a large physical impact from being applied to the fungus as in the case of using a pulverizer, and can prevent the fungus from being killed. In addition, since a certain amount of wind pressure acts, it is efficiently separated from the medium components, and it does not adhere to or agglomerate on the screen mesh etc. due to the air current, and it is transported without scattering even very light fungi such as spores and conidia. As a result, the screen mesh can be efficiently recovered and the recovery rate can be improved without deteriorating the working environment.

In the present invention, the screen mesh is formed in a cylindrical shape into which the microorganism culture is introduced on the inner peripheral side, and is disposed on the inner peripheral side of the screen mesh, and the rotation axis is the central axis of the screen mesh. It is preferable that the airflow is generated by rotating the rotating body using a rotating body having the above.
In the present invention, a rotating body having a rotating shaft is disposed at the central axis of a cylindrical screen mesh into which a microorganism culture is introduced on the inner peripheral side, and the wind speed is 0.3 m / second or more and 0.65 m / second by the rotation of the rotating body. Produces an airflow of less than a second.
This makes it possible to collect fungi at a high recovery rate with a simple structure provided with a rotating body, and it is not necessary to disintegrate the microorganism culture in advance, and the fungi are killed by the heat load and physical load during disintegration. This can be suppressed.

In the present invention, the rotating body includes a rotor blade provided with a plurality of support portions standing on the peripheral surface of the rotation shaft and a plurality of blade portions provided at a tip of the support portion and having a longitudinal direction along the rotation shaft. It is preferable to have a configuration provided.
In the present invention, a rotating body having a rotating blade provided with a plurality of blade portions whose longitudinal direction extends along the rotating shaft is used at the tip end of the column portion erected in the circumferential direction of the rotating shaft.
As a result, it is possible to generate an air flow with a wind speed of 0.3 m / second or more and 0.65 m / second or less while suppressing a large physical load from being applied to the fungus. The recovery rate can be improved.

In the present invention, the fungi include the genera Talalomyces, Penicillium, Pekiromyces, Gliocladium, Boberia, Recanicillium, Aspergillus, Trichoderma, Fusarium, Nomuraea, Verticillium, Mucor, Clado It is preferable that the structure is at least one selected from the group of the genus Sporium.
In this invention, the genera of Talaromyces genus, Penicillium genus, Pekirmyces genus, Gliocladium genus, Boberia genus, Recanicillium genus, Aspergillus genus, Trichoderma genus, Fusarium genus, Nomuraea genus, Verticillium genus, Mucor genus, Cladosporium genus Fungi selected from the group can be recovered at a high recovery rate.

In the present invention, it is preferable that the microorganism culture has a structure in which a powder and granular material are mixed.
In this invention, by mixing a granular material with a microorganism culture, fungi can be efficiently separated from the medium components by adhesion of the fungi to the granular material, and the recovery rate can be further improved.

The schematic perspective view which notched a part which shows the collection | recovery apparatus of one Embodiment which implements the collection | recovery method of the fungi of this invention.

Hereinafter, embodiments of the method for collecting fungi of the present invention will be described.
The present invention is not limited to the following embodiment.

[Microorganism culture]
The microbial culture used in the present invention is a composite of a culture medium component and a microorganism obtained by culturing a fungus as a microorganism using a solid medium or a liquid medium as a carrier and a nutrient source.
The microorganism culture in the present invention can be carried out by the same method as the usual microorganism culture method. There are no particular restrictions as long as the microorganisms grow, but solid culture methods using bran, wheat, barley, soybean flour, etc. can be used for mass cultivation.
However, in order to induce the formation of spores and conidia, it is desirable to adjust the culture conditions such as the composition of the medium, the pH of the medium, the culture temperature, the culture humidity, and the oxygen concentration so as to match the spore formation conditions. In addition, depending on the microorganism to be cultured, drying can be performed after completion of the culture, thereby improving the spore formation rate, spore preservation, and operability of the solid culture.

And it is preferable that a granular material is mixed with a microorganism culture. Although there is no restriction | limiting in particular as a granular material to mix, A clay mineral, resin, an inorganic fine powder, an organic compound, sugar, a food raw material fine powder, powdery surfactant, etc. are mentioned.
Examples of clay minerals include kaolinite and pyrophyllite.
Examples of the food raw material fine powder (organic fine powder) include wheat flour, soybean powder, and rice flour.
Examples of the powdered surfactant include an anionic surfactant, a cationic surfactant, an amphoteric surfactant, and a nonionic surfactant.
Examples of inorganic fine powder include charcoal fine powder, silica powder, various inorganic salts, and the like.
These particles preferably have a particle size of 0.05 to 500 μm, particularly 1 to 100 μm. Here, when the particle diameter is smaller than 0.05 μm, aggregation or scattering of the powder particles occurs, which may impair workability and spore recovery efficiency. On the other hand, when the particle size is coarser than 500 μm, although details will be described later, the permeability of the screen mesh is impaired, and the working efficiency and spore recovery efficiency may be reduced. From this, the particle size of the granular material is set to 0.05 μm or more and 500 μm or less.
In addition, when mixing the granular material, when mixing with a culture medium component, it may be mixed at any time such as immediately after the microorganism is recovered by a recovery device to be described later after the culture medium.

[Microorganisms]
There is no particular limitation on the microorganism used in the present invention, but the genera Talaromyces, Penicillium, Pekiromyces, Gliocladium, Boberia, Recanicilium, Aspergillus, Trichoderma, Fusarium, Nomuraea, Verticillium , At least any one microorganism selected from the group of the genus Mucor and Cladosporium.
In addition, the microbial culture which collect | recovers fungi, such as a spore and a conidia, with the collection | recovery apparatus mentioned later is not restricted to what cultured one kind of microorganism, The thing which cultured the several microorganisms selected from the said group is used. Alternatively, a microorganism culture in which a microorganism selected from the above group is cultured, and a plurality of microorganism cultures having different cultures may be used.

[Fungal recovery device]
The recovery apparatus for carrying out the method for recovering fungi of the present invention is to recover a fungus that is a spore or a conidia of a microorganism by applying an air current at a predetermined wind speed to the microorganism culture.
As shown in FIG. 1, the collection device 1 includes a housing 10, a cylindrical screen mesh 20 disposed in the housing 10, and a rotating body 30 disposed on the inner peripheral side of the screen mesh 20. It has. Specific devices include Pausifter (trade name: manufactured by Tsukasa Kogyo Co., Ltd.), Turbo Screener (product name: manufactured by Turbo Kogyo Co., Ltd.), US Shifter (trade name: Tokuju Kosakusho Co., Ltd.), and UX Shifter ( (Product name: Tokuju Works, Inc.) can be used. FIG. 1 shows the housing 10 and the screen mesh 20 that are partly cut out for convenience of explanation.

The casing 10 is formed of, for example, stainless steel, and has a cylindrical inner space 10A that communicates with an inlet (not shown) into which a microorganism culture is introduced from a hopper 11 and communicates with an outlet 12 that discharges fungi. doing. The housing 10 is provided with a lid 13 that opens and closes the internal space 10A. The lid 13 is provided with an outlet 13A through which culture residues that do not pass through the screen mesh 20 are discharged.
The material of the housing 10 is not limited to stainless steel, but a material that does not easily wear or corrode is suitable.

The screen mesh 20 is not particularly limited as long as it suits the purpose, but a resin mesh or a metal mesh can be used. In particular, those made of PET (polyethylene terephthalate) resin or nylon resin that have appropriate stretchability and are not easily broken by partial and instantaneous overload are suitable. In addition, as a metal thing, stainless steel is preferable at points, such as corrosion.
The screen mesh 20 is formed in a cylindrical shape, is coaxial within the internal space 10A of the housing 10, the inner peripheral side communicates with the input port, and the outer peripheral surface has a predetermined gap from the inner peripheral surface of the housing 10. It is arranged in a state of being separated. The screen mesh 20 can be taken out from the internal space 10A where the lid 13 is opened.
The screen mesh 20 is set to an opening that allows passage of fungi such as spores and conidia, and prevents passage of medium components of the microorganism culture. Specifically, by setting the opening to 1 mm or less, it is possible to separate fungi such as spores and conidia to be collected and culture residues such as medium components that become impurities. In particular, it is more desirable to set an opening for each microorganism to be collected.
Specifically, it is more preferably 0.08 mm or more and 1.0 mm or less in the case of Talamomyces genus, Penicillium genus, Pekyromyces genus and Aspergillus genus, and more preferably 0.05 mm or more and 0.8 mm or less in the case of Boberia genus and Recanicilium genus.
In addition, when an excipient | filler is mixed with the microorganism culture, an opening is 0.03 mm or more and 1.0 mm or less, Preferably it is 0.05 mm or more and 1.0 mm or less.

The rotating body 30 includes a rotating blade 31 and driving means 32 that rotates the rotating blade 31.
The rotary blade 31 is made of, for example, stainless steel, which is a material that is not easily worn or corroded. The rotary blade 31A is coaxially and rotatably disposed in the internal space 10A of the housing 10, and the rotary shaft 31A. The support portion 31B is erected on the peripheral surface of the support portion 31B, and the blade portion 31C is provided at the tip of the support portion 31B and has a longitudinal direction along the rotation shaft 31A. The blade portion 31C is disposed on the column portion 31B in a state twisted in the longitudinal direction. That is, the blade portion 31C is twisted so that the rotating airflow generated by the rotation of the rotary blade 31 becomes a vortex flowing from the inlet to the outlet 13A.
The drive unit 32 includes, for example, a motor 32A, and rotates the rotary blade 31 at a predetermined rotation speed to generate an air current.
Specifically, the rotation speed at which the wind speed when fungi such as spores and conidia pass through the screen mesh 20 generates an air flow of 0.3 m / second or more and 0.65 m / second or less is set. Here, when the wind speed is slower than 0.3 m / sec, spores and conidia are not sufficiently separated, and improvement in the recovery rate cannot be expected. In addition, when the wind speed is 0.65 m / sec, the physical impact applied to the spores and conidia increases, and the viable bacteria rate decreases, so that the recovery rate cannot be improved.
For this reason, the wind speed is set to 0.3 m / second or more and 0.65 m / second or less.

[Method of collecting fungi]
Next, a method for recovering fungi such as spores and conidia using the recovery device 1 will be described.

The driving means 32 is driven to rotate the rotary blade 31 at a predetermined rotational speed.
The rotation speed is such that the screen mesh 20 disposed in the housing 10 generates an air flow with a wind speed of 0.3 m / second or more and 0.65 m / second or less in a direction in which fungi such as spores or conidia pass. Is set.
In a state where this air flow is generated, the microorganism culture is introduced from the inlet.
The introduced microbial culture is dropped on the inner periphery of the screen mesh 20 where an airflow is generated, fungi are separated by the airflow, pass through the screen mesh 20, and pass through the discharge port 12 from the internal space 10A of the housing 10. And is collected as a fine powder fraction. A coarse fraction such as a culture residue that has not passed through the screen mesh 20 is discharged through the outlet 13A.
Here, the total amount of viable bacteria such as spores and conidia on the fine powder fraction side collected through the screen mesh 20 is 40% or more, preferably 50% or more with respect to the total viable bacteria of the microorganism culture. It is preferable. That is, it is suitable when fungi such as spores and conidia are used in industry and agriculture.

[Effects of Embodiment]
As described above, in the above-described embodiment, the microorganism culture is introduced into the airflow at a wind speed of 0.3 m / second or more and 0.65 m / second or less in the direction in which fungi such as spores and conidia pass through the screen mesh 20. doing.
As a result, fungi such as spores and conidia are separated from the medium components by wind pressure and collision between microbial cultures, and collision between added powder and microbial culture. Passed and collected.
Thus, a large physical impact as in the case of using a conventional pulverizer is not applied to fungi such as spores and conidia, and fungi such as spores and conidia can be prevented from being killed. In addition, since a certain amount of wind pressure acts, it can be separated from the medium components efficiently, and it can be transported without scattering even very light fungi such as spores and conidia without adhering or clumping to the screen mesh 20 etc. it can. For this reason, it can be efficiently recovered by passing through the screen mesh 20, and the recovery rate of fungi can be improved without causing deterioration of the working environment due to the dispersal of fungi.

And in the said embodiment, using the rotary body 30 which provided the rotary blade 31 which provided the blade | wing part 31C in which the longitudinal direction followed the rotation at the front-end | tip of the support | pillar part 31B standingly arranged in the circumferential direction of 31 A of rotating shafts. Yes.
Therefore, it is possible to generate an air flow with a wind speed of 0.3 m / second or more and 0.65 m / second or less while suppressing a large physical load from being applied to the fungus. The recovery rate can be improved. Furthermore, since the microbial culture is crushed by the rotating body 30, it is not necessary to perform the pulverization treatment in advance, and the fungus collection operation is facilitated, and the fungi can be collected more easily and efficiently.

Moreover, in the said embodiment, the granular material which can pass the screen mesh 20 is mixed with the microorganism culture.
For this reason, the fungi can be efficiently separated from the medium components by adhesion of the fungi to the granular material, and the fungal recovery rate can be further improved.

Furthermore, in the said embodiment, the blade | wing part 31C of the rotary blade 31 is formed in the state twisted, respectively.
As a result, the rotating airflow generated by the rotation of the rotary blade 31 becomes a vortex flow that flows from the inlet to the outlet 13A, and the input microorganism culture does not convect into the internal space 10A of the housing 10 for a long time. It is discharged appropriately from 13A. Therefore, it is possible to further prevent the disadvantage that the ratio of impurities increases due to contamination with fungi such as spores and conidia separated by pulverizing the medium components, and the fungus can be collected in a simple shape with the blade 31C twisted. The rate can be improved.

[Modification]
It should be noted that the present invention is not limited to the above-described embodiment, and modifications, improvements, and the like within the scope that can achieve the object of the present invention are included in the present invention.
Specifically, although the configuration using the recovery device 1 including the rotating body 30 has been illustrated, any configuration that generates an airflow at a predetermined wind speed can be applied.
For example, without using the rotator 30, various configurations that generate an air flow, such as compressed air such as a compressor and a cylinder, an intake pump, and a blower can be used. By using these air supply or intake configurations, the physical impact applied to the cells can be further reduced, and the viable cell rate of the cells can be improved.

  In addition, the specific structure and procedure for carrying out the present invention may be changed to other configurations as long as the object of the present invention can be achieved.

Hereinafter, the experiment which collect | recovered fungi on various conditions using the collection | recovery apparatus 1 provided with the rotary body 30 is demonstrated.
The present invention is not limited to the following examples.

[Culture of microorganisms]
Various microorganisms shown in Table 1 below were used as microorganisms.
Specifically, Y-9401 strain (FERM P-15816) was used as the Tallalomyces flavus (microorganism 1). As the Pekiromyces tenuipes (microorganism 2), the OT10 strain (FERM AP-21795) was used. F-263 strain was used for Boberia vaciana (microorganism 3). OT14 strain (FERM AP-21796) was used as Recanicillium regani (microorganism 4). Aspergillus oryzae (microorganism 5) used was isolated and obtained from soil.

As the medium component, MY-20 medium prepared by dissolving MY-20 medium component in distilled water, pouring it into a Sakaguchi flask, sealing with a silico stopper, and sterilizing at 121 ° C. for 15 minutes in an autoclave was used.
Each microorganism was cultured with shaking in MY-20 medium at 30 ° C. for 5 days to prepare a culture solution.
Further, the bran having a water content of 55% by adding water was sterilized by autoclave, and then thoroughly mixed to obtain a solid medium. This solid medium was inoculated with a culture solution of each microorganism previously cultured, and cultured at 28 ° C. for 7 days. Then, it dried at 30 degreeC for 3 to 7 days, and obtained the microorganism culture.
Thus the spore concentration of each microorganism culture obtained by was measured to be ^{4 × 10 9 cfu / g~9 ×} 10 9 cfu / g.

Here, analysis of the spore concentration of the microorganism culture was performed by the following method.
1 g of the microbial culture was accurately weighed and added to 100 ml of a sterilized 0.01% Tween solution and stirred sufficiently to prepare a microbial suspension. 1 ml of this microbial suspension is added to 9 ml of a sterilized 0.01% Tween solution and stirred sufficiently to prepare a 1/10 dilution. The preparation operation of the obtained 1/10 preparation solution is repeated to prepare a 10 ⁻⁵ diluted solution of the microorganism suspension.
Then, 5 ml of potato dextrose agar plate was coated with 0.1 ml of 10 ⁻⁵ dilution and cultured at 30 ° C. for 2 to 3 days. The number of target microbial colonies appearing on each plate after the culture is counted, and an average value of five is calculated. Then, the spore concentration (CFU (colony forming unit) concentration) [cfu / g] in the microorganism culture was calculated using the following calculation formula (1).
In addition, when the CFU concentration of the microorganism culture was low, it was prepared by reducing the number of dilution stages in the preparation work of the 1/10 preparation solution.
(Average number of colonies on plate) × 100 × 10 ⁵ × 10 (1)

[Recovery method]
For recovering the microorganism spores, each apparatus shown in Table 2 below was used.
Specifically, a microbial culture (culture in Table 3) and a mixture containing powder prepared by premixing the microbial culture with powder (mixture in Table 3) were used as samples. . In addition, the granular material was comprised with kaolinite and pyrophyllite, and used the clay mineral whose particle size is 1 micrometer or more and 100 micrometers or less.
Then, the openings and operating conditions of each device were changed as appropriate, and spores were collected from the prepared microorganism culture.
The results are shown in Table 3 below.
In Table 3, microorganisms are those shown in Table 1, and devices are those shown in Table 2. Moreover, in the fine powder fraction recovery rate, the mass is the ratio [%] of the mass of the fine powder fraction collected through the screen mesh 20 to the mass of the sample, and the viable cell is the fine powder fraction relative to the total CFU of the microorganism culture as the sample. It is the ratio [%] of the total amount of CFU in minutes. Similarly, in the coarse-grain fraction residual rate, the mass is the ratio of the mass of the coarse-grain fraction that has not passed through the screen mesh 20 to the mass of the sample [%], and the living bacteria is the CFU of the microorganism culture that is the sample. It is the ratio [%] of the CFU total amount of the coarse-grained fraction to the total amount. In the loss rate, the mass is the ratio of the mass obtained by subtracting the fine powder fraction and the coarse fraction from the total amount of the sample [%], and the viable bacteria subtracts the total CFU content of the fine powder fraction and the coarse fraction from the total CFU amount of the sample. It is a ratio [%] of the total amount of CFU.

From the results shown in Table 3, it was found that in Comparative Examples 1 and 6 using a vibrating sieve, many spores remained on the coarse fraction side.
In Comparative Examples 2 to 5 and 7 using a pulverizer such as a pin mill, a power mill, and a quick mill, although the recovery rate of live spores on the fine powder fraction side is improved as compared with the case of using a vibrating sieve, It can be seen that the loss of bacteria is high, the ratio of scattering and particularly dead is high, the impact is great, and the recovery rate cannot be improved.
On the other hand, in Examples 1-15, the spore was collect | recovered by the high recovery rate and the high viable cell rate by classifying through the screen mesh 20 with the airflow of wind speed 0.30 m / sec or more.
And it was able to collect | recover a spore with the high collection | recovery rate and the high viable cell rate in the range of 0.08 mm to 1.0 mm as an opening of the screen mesh 20. In the Tallaromyces sp. (Microorganism 1), in the range of 0.1 mm or more and 0.7 mm or less, mixing of unnecessary medium components was suppressed, and a particularly high spore recovery rate was exhibited. In the genus Pekyromyces, a high spore recovery rate was shown at 0.5 mm. In the genus Boberia, a high spore recovery rate was exhibited at 0.08 mm to 0.7 mm. In the genus Recanicillium, a high spore recovery rate was shown at 0.08 mm. Aspergillus spp. Showed a high spore recovery rate at 0.1 mm.

DESCRIPTION OF SYMBOLS 1 ... Recovery apparatus 30 ... Rotating body 31 ... Rotating blade 31A ... Rotating shaft 31B ... Strut part 31C ... Rotating blade

Claims (5)

A recovery method for recovering fungi cultured from a microorganism culture,
Using a screen mesh through which the fungi can pass and culture residues of the microbial culture cannot pass,
The method for recovering fungi, wherein the microorganism culture is introduced into an air current having a wind speed of 0.3 m / sec or more and 0.65 m / sec or less in a direction in which the fungi pass through the screen mesh. The method for collecting fungi according to claim 1,
The screen mesh is formed in a cylindrical shape into which the microorganism culture is introduced on the inner periphery side,
Using a rotating body that is disposed on the inner peripheral side of the screen mesh and has a rotation axis at the center axis of the screen mesh,
The method for collecting fungi, wherein the air flow is generated by rotating the rotating body. In the method for collecting fungi according to claim 2,
The rotating body includes a rotary blade provided with a plurality of support portions standing on the peripheral surface of the rotation shaft and a plurality of blade portions provided at the tip of the support portion and having a longitudinal direction along the rotation shaft. A method for collecting fungi. In the method for collecting fungi according to any one of claims 1 to 3,
The fungi include those of the genus Tallomyces, Penicillium, Pekyromyces, Gliocladium, Boberia, Recanicilium, Aspergillus, Trichoderma, Fusarium, Nomuraea, Verticillium, Mucor, and Cladosporium A method for collecting fungi, wherein the fungus is at least one selected from the group. In the method for collecting fungi according to any one of claims 1 to 4,
The method for collecting fungi, wherein the microorganism culture is mixed with a powdered product.