JP5900906B2 - Method for producing cosmetic base and skin cosmetic - Google Patents

Description

  The present invention relates to a method for producing a cosmetic base that promotes absorption of collagen into the skin stratum corneum, and a skin cosmetic containing the cosmetic base.

  In general, collagen is a fibrous protein that constitutes our body, and in the skin tissue, the main component of the dermis is collagen. Normal skin collagen plays a role of so-called skin support, firmly supports the epidermis, and brings elasticity and firmness of the skin. This collagen has a triple helix structure, and its structure shape is considered to have a very high water retention capacity and a high amount of bound water.

  Conventionally, many skin cosmetics and supplements have been developed in anticipation of the moisturizing effect due to the high water retention ability of this collagen, but the average molecular weight of collagen is very high, about 200,000 to about 400,000, For improved solubility in each formulation and skin penetration, gelatin that has been improved in solubility by heat treatment, or low molecular gelatin obtained by cutting the gelatin (so-called hydrolyzed collagen) It is supported by.

Takada, M., Yuzuriha, T., Katayama, K., Iwamoto, K., Sunamoto, J., Biochim. Biophys. Acta, 802, 237 (1984) Sunamoto, J., Iwamoto, K., Takada, M., Yuzuriha, T., Katayama, K., In Polymers in Medicine; Chillini, E., Giusti, PA, Eds. Plenum Publishing Co. New York, 1984, p.157., Moellerfeld, J., Press, W., Ringsdorf, Hamazaki, H., Sunamoto, J., J. Biochim. Biophys. Acta, 857, 265 (1986), Sunamoto, J., Sato, T., Taguchi, T., Hamazaki, H., Macromolecules, 25, 5567 (1992)

  In these papers, hydrophobic long-chain alkyl groups or cholesterol are chemically bonded to polysaccharides such as pullulan, and these hydrophobic group-modified polysaccharides and liposomes are mixed to form hydrophobic groups in the liposome membrane. Liposomes that are spontaneously incorporated into and coated with a hydrophobic group-modified polysaccharide. Thereby, it is the technique that the leakage of the liposome inclusion is suppressed and stabilized. Further, this coating makes it possible to measure the stability of liposomes in blood. However, regarding collagen, since a heating step is introduced in the process of introducing a hydrophobic group into a collagen molecule, the triple helix structure of collagen is destroyed, so the techniques shown in these papers are not applied. Also, the coating method and purpose are different. Therefore, it can be said that it is a prior art document from the viewpoint of coating a liposome with a polymer substance, but is clearly different from the present patent in coating technology and / or purpose.

  An object of the present invention is to provide a cosmetic base that can absorb collagen into the skin stratum corneum without impairing the triple helix structure inherent in the collagen, improving the affinity to the skin and the storage property. Then, it is intended to improve the moisture retention ability of the skin when the cosmetic base is used as a skin cosmetic and to improve the amount of bound water, and to exhibit the moisturizing effect more effectively.

  As a result of extensive research, collagen modification as a cosmetic base was achieved by adsorbing water-soluble collagen, which maintains a triple helical structure, on the surface of liposomes having a bilayer structure similar to the stratum corneum intercellular lipid structure. The present inventors have found that the above problems can be solved by using liposomes, and have completed the present invention.

That is, the present invention relates to the following.
(1) A method for producing a cosmetic base that promotes absorption of collagen into the skin stratum corneum, wherein phosphatidylethanol having a positive charge is added to phosphatidylcholine, which is the main component of the liposome membrane , in an environment below the denaturation temperature of the collagen. At least one of dicetyl phosphate, phosphatidylinositol, phosphatidyl glycerol and phosphatidic acid having a surface charge of + by adding at least one of an amine and a dialkyl ether ammonium salt or having a -charge on phosphatidylcholine which is the main component of the liposome membrane To the aqueous solution of liposomes whose surface charge was controlled to-by adding one, water-soluble collagen whose charge was controlled to the opposite side of the liposomes while maintaining the triple helical structure was dropped. Stir, Method for producing a cosmetic base for causing the soluble collagen on the surface of serial liposomes adsorbed by electrostatic interactions.
(2) The method for producing a cosmetic base according to (1), wherein the water-soluble collagen is controlled to have a positive charge by an enzyme treatment.
(3) The method for producing a cosmetic base according to (1), wherein the water-soluble collagen is controlled to be negatively charged by alkali treatment.
(4) A skin cosmetic using a cosmetic base that promotes the absorption of collagen into the skin stratum corneum , comprising at least phosphatidylethanolamine and a dialkyl ether ammonium salt having a positive charge on phosphatidylcholine which is a main component of the liposome membrane. The surface charge is increased by adding one, or by adding at least one of dicetyl phosphate, phosphatidylinositol, phosphatidylglycerol, and phosphatidic acid having a negative charge to the phosphatidylcholine which is the main component of the liposome membrane. the - on the control surface of the liposome, while holding the triple helical structure, and was adsorbed by electrostatic interaction with water-soluble collagen which is controlled to the opposite pole to the liposome charge, As the cosmetic base Skin cosmetic, wherein the collagen-modified liposome, and a-law including the hydrolysis elastin.
(5) A skin cosmetic using a cosmetic base that promotes the absorption of collagen into the skin stratum corneum , comprising at least phosphatidylethanolamine and a dialkyl ether ammonium salt having a positive charge on phosphatidylcholine, which is the main component of the liposome membrane. The surface charge is increased by adding one, or by adding at least one of dicetyl phosphate, phosphatidylinositol, phosphatidylglycerol, and phosphatidic acid having a negative charge to the phosphatidylcholine which is the main component of the liposome membrane. the - on the control surface of the liposome, while holding the triple helical structure, and was adsorbed by electrostatic interaction with water-soluble collagen which is controlled to the opposite pole to the liposome charge, As the cosmetic base Skin cosmetic, wherein the collagen-modified liposome, and a-law contains a hyaluronic acid.
(6) The skin cosmetic according to (4) or (5), which is stored by freeze-drying.

  According to the present invention, chemical treatment for improving solubility in water and dispersibility is not performed, and the charge of water-soluble collagen in a stable state while maintaining a triple helical structure is opposite to that of liposomes. When the solution is dripped into the liposome aqueous solution under an environment below the collagen denaturation temperature, the collagen itself adsorbs to the liposome in a stable state, and the collagen that maintains its high water retention capacity is adsorbed. The liposomes can be produced. Therefore, if the cosmetic base produced by this production method is used, collagen is absorbed into the skin stratum corneum together with liposomes, and the skin cosmetics having an excellent water retention effect on the skin are provided by the high moisture retention ability of the collagen. It becomes possible.

  Normally, water-soluble collagen forms a complex by coexisting with hyaluronic acid due to its charged state, resulting in a non-uniform solution and impairing the original water retention function. However, according to the present invention, the structure of the water-soluble collagen can be kept stable by adsorbing the water-soluble collagen to the liposome whose surface charge has been controlled in advance, and is described above in the presence of hyaluronic acid and hydrolyzed elastin. It became possible to obtain a uniform and stable composition without forming a complex.

  Further, in order to maintain the triple helical structure of collagen, the dosage form in which free water such as an aqueous solution, a gel, and an emulsion is present, collagen denaturation temperature (fish: 20-30 ° C., mammal: 36- (38 ° C.) or less, the product can be stabilized by being manufactured and stored. On the other hand, free water is reduced to 0% by lyophilization, and the triple helix structure can be maintained even at the denaturation temperature or higher. When used, an aqueous solution is added to disperse the lyophilized composition, and then applied to the skin. It can be used as a preparation in which the collagen triple helix structure is stabilized even when it is prepared and used at the denaturation temperature or below. Here, the triple helix structure of collagen collapses above the denaturation temperature due to the presence of free water and becomes gelatin, but the denaturation temperature is not limited as long as it is bound water even if water is present. Even above, the triple helical structure does not collapse.

  The method for producing the cosmetic base of the present invention is modified to aqueous collagen by adsorbing water-soluble collagen whose charge is previously controlled to liposomes whose surface charge is previously controlled in an environment below the denaturation temperature of collagen. Liposomes are produced as cosmetic bases.

  To control the surface charge of liposomes to +, phosphatidylcholine (surface charge is 0), which is the main component of the liposome membrane, is added with positively charged phosphatidylethanolamine, dialkyl ether ammonium salt, etc. Get.

  In addition, in order to control the surface charge of the liposome to-, phosphatidylcholine (surface charge is 0), which is the main component of the liposome membrane, is added with -charged dicetyl phosphate, phosphatidylinositol, phosphatidylglycerol, phosphatidic acid, etc.- A charge-controlled liposome is obtained.

  In order to control the charge of water-soluble collagen adsorbed on the surface of the liposome, a positively charged collagen can be obtained by enzymatic treatment, and a negatively charged collagen can be obtained by alkaline treatment.

  The molecular weight of the liposome may be appropriately selected from about 10,000 to about 3 million on average.

  The water-soluble collagen adsorbed on the surface of the liposome may be adsorbed at a molar ratio of liposome: collagen = 1: 0.01 to 1: 100, preferably 1: 0.1 to 1:10.

  The water-soluble collagen used in the present invention is a collagen in a state where a triple helical structure is maintained without chemical treatment for improving solubility in water and dispersibility. The molecular weight of the water-soluble collagen is preferably about 200,000 to about 400,000. Furthermore, atelocollagen from which telopeptides at both ends of the collagen molecule have been removed is preferred for eliminating allergic reactions that are rarely caused by telopeptides.

  Usually, in order to improve the complexity of production and storage and the solubility in water, gelatin that has been heat-treated or the original collagen that has been reduced in molecular weight by chemical or enzymatic treatment is used. However, since the water-soluble collagen used in the present invention is in a state where the original triple helical structure of collagen is maintained, the water-retaining ability is high.

  The liposome in the present invention is a closed vesicle composed of a single layer or a plurality of layers of phospholipid obtained by mixing phospholipid and water, and has high affinity with cells. Liposome membrane-forming substrates include phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol, phosphatidic acid, lysophosphatidylcholine, sphingomyelin, egg yolk lecithin, soy lecithin, etc. Lipids, cholesterols such as cholesterol, cholesterol alkyl esters, phytosterols, phytosterol alkyl esters, glyceroglycolipids, acyl glucosides such as cetyl galactoside, dicetyl phosphate, dialkyl type synthetic surfactants, N-acyl-sphingosine or sulfuric acid thereof One or a mixture of two or more of ester, N-acylsphingoglycolipid and N-higher acylglutathione I can get lost. In addition, a polyhydric alcohol, a higher alcohol, a higher fatty acid, and the like can be blended as necessary to stabilize the liposome or improve the phase transition temperature.

  In addition, you may make a liposome encapsulate a hydrophilic medicinal ingredient and / or a lipophilic medicinal ingredient. Examples of hydrophilic medicinal components and lipophilic medicinal components include antioxidants, antibacterial agents, anti-inflammatory agents, blood circulation promoters, whitening agents, skin roughening agents, anti-aging agents, moisturizers, hormone agents, vitamin agents, pigments , And proteins.

  Examples of the liposome production method include an ultrasonic method, an ethanol injection method, a cholic acid removal method, a reverse phase evaporation method, an extruder method, and a microfluidizer method.

  Furthermore, by coexisting with hyaluronic acid and / or hydrolyzed elastin having a high moisturizing effect, it becomes possible to produce a highly functional water-retaining cosmetic with excellent moisture retention ability. Turnover can be adjusted.

  Elastin, together with collagen, is an important protein in connective tissues such as the ligament ligament, blood vessels, and skin, but has the property of hardly dissolving in water. The hydrolyzed elastin in the present invention is hydrolyzed elastin whose solubility in water is improved by enzymatic treatment or oxalic acid treatment of the knot ligament.

  In the present invention, a collagen-modified liposome as a cosmetic base manufactured by the above-described manufacturing method, or a cosmetic base prepared by blending hyaluronic acid and / or hydrolyzed elastin into the collagen-modified liposome, It can be a cosmetic.

  The skin cosmetic of the present invention is not particularly limited, but includes, for example, cosmetics, emulsions, creams, lotions, packs, facial cleansers, massages, liquid foundations, emulsion foundations, powder foundations and the like. And cosmetics for body.

  In the skin cosmetic of the present invention, the blending standard of the collagen-modified liposome is 0.001 to 10 wt%, preferably 0.01 to 5 wt% in the skin cosmetic.

In the skin cosmetic of the present invention, the blending ratio of collagen-modified liposome and hyaluronic acid and / or hydrolyzed elastin is used at an arbitrary ratio, but the total blending ratio of hyaluronic acid and / or hydrolyzed elastin is 0.001-10 wt% per skin cosmetic is preferable, and 0.005-1 wt% is more preferable. If the amount exceeds this, dispersibility in water becomes difficult, which may adversely affect the stability of separation, discoloration, odor change, and the sensory properties of skin cosmetics.
In addition to the essential components described above, components to be blended in ordinary cosmetics, such as oils and fats, hydrocarbons, waxes, fatty acids, synthetic esters, alcohols, powders, surfactants, thickeners , Polymer compounds, gelling agents, UV absorbers, UV scattering agents, antioxidants, dyes, pigments, preservatives, fragrances, chemicals, water, etc. are appropriately selected and used within a range not impairing the effects of the present invention. Can do.

  Examples of the fats and oils include jojoba oil, castor oil, olive oil, soybean oil, coconut oil, palm oil, cacao oil, mink oil, turtle oil and the like.

  Examples of hydrocarbons include liquid paraffin, petrolatum, microcrystalline wax, squalane and the like.

  Examples of waxes include beeswax, lanolin, carnauba wax, and candelilla wax.

  Examples of fatty acids include myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, lauric acid and the like.

  Synthetic esters include isopropyl myristate, isopropyl palmitate, butyl oleate, myristyl myristate, octyldodecyl myristate, propylene glycol monostearate, myristyl lactate, isostearyl malate, glyceryl monostearate, distearyl dimethyl chloride Ammonium etc. are mentioned.

  Oils and fats, hydrocarbons, waxes, fatty acids and synthetic esters are usually combined at a rate of 0 to 30 w / w% per skin cosmetic.

  Examples of alcohols include ethanol, 1,3-butylene glycol, propylene glycol, lauryl alcohol, cetanol, stearyl alcohol, and oleyl alcohol. Alcohols are usually blended at a rate of 0 to 25 w / w% per skin cosmetic.

  Surfactants include polyglycerin fatty acid esters, sorbitan fatty acid esters, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene alkyl ethers, coconut oil fatty acid monoethanolamide, polyoxyethylene cured Castor oil, sodium lauryl sulfate, polyglutethylene isostearate polyoxyethylene glyceryl, sodium alkylbenzenesulfonate, polyoxyethylene stearyl ether, dialkylsulfosuccinic acid, cetylpyridinium bromide, -n-octadecyltrimethylammonium chloride, monoalkyl phosphoric acid, N -Acyl sultamic acid, N-acyl glutamine, sucrose fatty acid ester, polyoxyethylene sorbitan monostearate, polyoxyethylene Examples include sodium lauryl ether sulfate, polyoxyethylene reduced lanolin, sugar ester and the like. The surfactant is usually blended at a rate of 0 to 10 w / w% per skin cosmetic.

  Examples of the thickener include carboxyvinyl polymer, methylpolysiloxane, dextran, carboxymethylcellulose, carrageenan, hydroxypropylmethylcellulose and the like. The thickener is usually blended at a rate of 0 to 5 w / w% per skin cosmetic.

  Examples of the humectant include glycerin, propylene glycol, 1,3-butylene glycol, pyroglutamic acid, acetylglutamic acid, procyanidin and the like. The humectant is usually blended at a rate of 0 to 25% per skin cosmetic.

  Examples of the preservative include benzoic acid, salicylic acid, dehydroacetic acid or salts thereof, phenols of paraoxybenzoic acid esters, triclosan halocarban, and the like. The preservative is usually blended at a rate of 0 to 0.3 w / w% per skin cosmetic.

  Any fragrance may be used as long as it is normally used in cosmetics.

  Examples of the pigment include iron oxide, titanium dioxide, zinc oxide, kaolin and talc. The pigment is usually blended at a rate of 0 to 5 w / w% per skin cosmetic.

  Drugs include wheat germ oil, vitamin A, vitamin B2, vitamin E, magnesium or sodium ascorbate-2-phosphate, D-pantotail alcohol, dipotassium glycyrrhizinate, glutathione, UV absorber, chelating agent, plant extract And microbial metabolites / extracts. The drug is usually blended at a rate of 0 to 5 w / w% per skin cosmetic.

  Examples of water include tap water, mineral water, brine, seawater, deep ocean water, ultrapure water, polar ice-derived water, mineral water, and purified water. Water is blended in an arbitrary ratio.

  In addition, the deep sea water of the present invention is a natural water that is formed by forming a layer by submerging the surface seawater, and is low temperature, clean and rich in nutrient salts, and its intake sea area is 1000 to 4000 m, preferably 2000. It is -3000m, and a water intake depth is 250-500m, Preferably it is 300-400m.

  Deep sea water contains a lot of minerals, so it has excellent moisture retention and good penetration feeling, which promotes penetration of active ingredients in cosmetics into the skin. Conceivable.

  The dosage form of the skin cosmetic of the present invention is arbitrary, and may take, for example, a solubilizing emulsifier form or a dispersion form.

  In the present invention, when storing above the denaturation temperature of collagen, the above-mentioned cosmetic base and skin cosmetic may be lyophilized. In the freeze-drying method, a phase change occurs on the individual liquid equilibrium line at a pressure below the triple point, and moisture is directly removed by vaporization from the solid phase (ice) by sublimation to remove moisture, that is, dry. Such a dehydration drying method by sublimation is called vacuum freeze-drying. By using this method, it is possible to stabilize an active ingredient that is easily denatured by temperature in the presence of water. In the present invention, a preparation comprising a solid content from which moisture has been removed by freeze-drying a collagen-modified liposome aqueous solution is prepared, and the aqueous solution is added and shaken at the time of use to obtain a uniform dispersion of collagen-modified liposomes. After coating on the part of the face that is worrisome, the dispersion is stored under 20 ° C. By doing so, collagen having a triple helical structure can be stably supplied, and collagen can be absorbed into the skin stratum corneum as the liposome permeates.

  Examples and test examples of the present invention are shown below.

  Note that the present invention is not limited to the embodiments.

An aqueous solution of liposome modified with water-soluble collagen 0.9% by weight yolk lecithin Extruder method (MJHope, MBBally, G. Webb and PRCullis, Biochim. Biophys. Acta, vol. 812, 55 (1985)) Prepared. The liposome membrane was composed of egg yolk lecithin: cholesterol: dicetyl phosphate = 4: 4: 2 (molar ratio), and was negatively charged at pH 7.0. The average molecular weight of the liposomes was about 500,000 to about 1 million. At 18 ° C., the aqueous solution of atelocollagen having a molecular weight of about 300,000 was added dropwise to the aqueous solution of liposomes described above while stirring the aqueous solution and a 0.5 wt% aqueous solution of atelocollagen having a neutral and positive charge at a ratio of 1: 5. By stirring with a disperser for 1 hour, an aqueous liposome solution modified with water-soluble collagen was obtained. Stored at 5 ° C.

Liposome aqueous solution consisting of 1.2 wt% egg yolk lecithin modified with water-soluble collagen was applied to the extruder method (MJHope, MBBally, G. Webb and PRCullis, Biochim. Biophys. Acta, vol. 812, 55 (1985)). Prepared. The liposome membrane was composed of egg yolk lecithin: cholesterol: phosphatidylethanolamine = 4: 4: 2 (molar ratio), and was positively charged at pH 7.0. The average molecular weight of the liposomes was about 500,000 to about 1 million. At 18 ° C., the aqueous solution of atelocollagen is added dropwise to the aqueous solution of liposomes described above with stirring at a ratio of 2: 5 with this aqueous solution and a neutral-charged molecular weight of about 300,000 atherocollagen at a ratio of 2: 5, By stirring with a disperser for 1 hour, an aqueous liposome solution modified with water-soluble collagen was obtained. Stored at 5 ° C.

Lyophilized preparation comprising liposomes surface-modified with water-soluble collagen and hyaluronic acid, hydrolyzed elastin 40 parts by weight of an aqueous liposome solution surface-modified with water-soluble collagen obtained in Example 1
At 17 ° C., 10 parts by weight of 0.5 wt% sodium hyaluronate aqueous solution was added dropwise, then 50 parts by weight of 0.1 wt% hydrolyzed elastin aqueous solution was added dropwise, and the mixture was further stirred at the same temperature for 30 minutes. In 90 parts by weight of this solution, 10 parts by weight of mannitol was dissolved with stirring. The resulting solution was lyophilized to obtain a lyophilized composition. Water was added to this composition to obtain an aqueous solution consisting of liposomes whose surface was modified with water-soluble collagen, hyaluronic acid, and hydrolyzed elastin, but there was no precipitation caused by the normally observed complex of collagen and sodium hyaluronate. I understood.

  The stability of the triple helix structure of the water-soluble collagen of this lyophilized composition was observed at 45 ° C. The stability of the triple helix structure was confirmed by SDS-polyacrylamide gel electrophoresis and comparison with the viscosity of the same sample stored below the denaturation temperature (viscosity decreases when the triple helix structure collapses).

  From the results shown in Table 1, it was found that the stability of the lyophilized composition was clearly excellent and sufficiently high enough to withstand commercialization.

Liposome serum liquid yolk lecithin (1.5 wt%), cholesterol (0.3 wt%), dicetyl phosphate (0.2 wt%), oleic acid (0.5 wt%), stearyl alcohol (0. 3 wt%), glyceryl stearate (2.0 wt%), arginine (0.7 wt%), 1,3-butylene glycol (5 wt%), dipropylene glycol (2 wt%), glycerin (3 wt%), corn oil ( 1.5 wt%), avocado oil (1 wt%), polyglyceryl-5 oleate (0.5 wt%) and purified water (adjusted to 100 wt%) were used for the liposomal serum for the LLC method (T. Kaneko and H. Sagitani, Colloids & Surfaces, vol.69, 125 (1992)). At 15 ° C., 10 parts by weight of an aqueous solution of atelocollagen having a positive charge of 0.5 wt% was added dropwise to 80 parts by weight of this cosmetic liquid with stirring. The stirring was continued for 10 minutes, and then 0.5% sodium hyaluronate 10 Part by weight was added dropwise with stirring, and stirring was further continued for 10 minutes to obtain the desired cosmetic liquid. As a result of microscopic observation, it was found that the normally observed complex of collagen and sodium hyaluronate did not occur.

Test example 1
Test for absorbability of collagen into the stratum corneum Using the collagen aqueous solution having the same concentration (0.083 wt%) as the liposome aqueous solution obtained by surface modification of the water-soluble collagen obtained in Example 1, the collagen absorbability to the human skin stratum corneum Tested. The number of n was eight, and the sample was applied to the in-arm measurement treated with an acetone / ether (1/1) solution, and the sample was taken 15 hours after occlusion with a patch size and measured.
Quantitative determination of collagen absorbed into the stratum corneum: 15 hours after application, the sample remaining on the skin was wiped off with purified water and ethanol, and the stratum corneum was collected by a tape stripping method (2-5 layers, 6-10 layers and 11). -15 layers were collected and used as measurement samples). The first tape was removed from the collected tapes, and the second and subsequent tapes were used as measurement samples. The water-soluble component of the collected tape was collected, the hydroxyproline content in the component was measured, and the amount of absorption was calculated in terms of collagen (the amount of hydroxyproline in the uncoated control part was measured and used as a blank).

  From the results in Table 2, it was found that collagen is hardly absorbed into the stratum corneum in an aqueous solution, but absorption into the stratum corneum is promoted by adsorbing to the liposomes.

Test example 2
The test conditions for the moisture retention test of the aqueous solution of Example 2 are as follows.
(Experimental conditions)
The inner side of the upper arm was treated with an SDS solution to form rough skin, and two days later, each sample started to be applied once in the morning and evening, and measurement was started.
The keratin water content was measured after the subject was allowed to stand for 30 minutes in a room controlled to 20 ± 1 ° C./40±5 RH% with n = 6 persons. The measurement results are shown in Table 2. Measuring equipment: Skin Surface Hygrometer [SKICON-200] (Measures stratum corneum moisture in μS)

  From Table 3, it turned out that the aqueous solution of Example 2 has a clear rough skin improvement effect clearly from a control part.

Test example 3
Experimental conditions for the skin rejuvenation effect of the cosmetic liquid of Example 4 are as follows.

(Experimental conditions)
The inner side of the upper arm was treated with an acetone / ether (1/1) solution (treated twice at an interval of 3 days) to form rough skin, and from the next day, each sample was applied once in the morning and evening, and measurement was started. The skin viscosity was measured after the subject was allowed to stand for 60 minutes or more in a room controlled to 20 ± 1 ° C./40±5 RH% with n = 8. The results are shown in Table 3. Measuring instrument: Dermal Torque Meter (manufactured by Dia-Stron) The closer the DTM value (Ur / Ue) is to 1, the higher the viscosity of the skin and the better the return.

  From Table 4, it was found that the cosmetic liquid of Example 4 can promote a quicker recovery than the control with a significant difference.

  The cosmetic base of the present invention is capable of maintaining the structure of water-soluble collagen stably, and does not form a complex even in the presence of hyaluronic acid or hydrolyzed elastin. Can do.

Claims (6)

A method for producing a cosmetic base that promotes absorption of collagen into the skin stratum corneum, wherein the phosphatidylcholine, which is a main component of the liposome membrane, has a positive charge in an environment below the denaturation temperature of the collagen. Add at least one of ether ammonium salt to add surface charge to +, or add at least one of dicetyl phosphate, phosphatidylinositol, phosphatidylglycerol, phosphatidic acid having -charge to phosphatidylcholine which is the main component of liposome membrane By adding dropwise water-soluble collagen whose surface charge is controlled to-to the aqueous solution of liposomes while maintaining the triple helical structure and whose charge is controlled to the opposite side of the liposomes, the mixture is stirred. The lipo A method for producing a cosmetic base, wherein the water-soluble collagen is adsorbed on the surface of a some by electrostatic interaction. The method for producing a cosmetic base according to claim 1, wherein the water-soluble collagen is controlled to be + charged by an enzyme treatment. The method for producing a cosmetic base according to claim 1, wherein the water-soluble collagen is controlled to be negatively charged by an alkali treatment. A skin cosmetic using a cosmetic base that promotes absorption of collagen into the skin stratum corneum , comprising at least one of phosphatidylethanolamine and a dialkyl ether ammonium salt having a positive charge on phosphatidylcholine which is a main component of a liposome membrane. By adding the surface charge to +, or by adding at least one of dicetyl phosphate, phosphatidylinositol, phosphatidylglycerol and phosphatidic acid having a charge to the phosphatidylcholine which is the main component of the liposome membrane, the surface charge is- the control surface of the liposome, while holding the triple helical structure, and was adsorbed by electrostatic interaction with water-soluble collagen which is controlled to the opposite pole to the liposome charge, the cosmetic Coller as a base Skin cosmetic, wherein the down-modified liposome, and a-law including the hydrolysis elastin. A skin cosmetic using a cosmetic base that promotes absorption of collagen into the skin stratum corneum , comprising at least one of phosphatidylethanolamine and a dialkyl ether ammonium salt having a positive charge on phosphatidylcholine which is a main component of a liposome membrane. By adding the surface charge to +, or by adding at least one of dicetyl phosphate, phosphatidylinositol, phosphatidylglycerol and phosphatidic acid having a charge to the phosphatidylcholine which is the main component of the liposome membrane, the surface charge is- the control surface of the liposome, while holding the triple helical structure, and was adsorbed by electrostatic interaction with water-soluble collagen which is controlled to the opposite pole to the liposome charge, the cosmetic Coller as a base Skin cosmetic, wherein the down-modified liposome, and a-law contains a hyaluronic acid. The skin cosmetic according to claim 4 or 5, wherein the skin cosmetic is stored by freeze-drying.