JP5950733B2 - Automatic analyzer - Google Patents

Description

  The present invention relates to an automatic analyzer that performs qualitative and quantitative analysis of biological samples such as blood and urine.

  Automatic analyzers perform qualitative and quantitative analysis by adding and reacting with reagents that react specifically with specific components in biological samples such as blood and urine, and measuring the absorbance and luminescence of the reaction solution. Is.

  As such an automatic analyzer, for example, in Patent Document 1 (Japanese Patent Laid-Open No. 2009-204445), a reaction vessel containing a mixed solution of a sample and a reagent is immersed in temperature-adjusted reaction vessel water to react the reaction solution. A technique for qualitative / quantitative analysis of a target component by controlling the reaction and measuring the optical characteristics of the reaction solution is disclosed.

JP 2009-204445 A

  In the above prior art, optical characteristics are measured in a state in which a reaction vessel containing a reaction solution is immersed in the reaction vessel water, and fine bubbles (micro bubbles) generated by dissolved air in the reaction vessel water are the result of the measurement. There are concerns about adverse effects on stability and accuracy. Therefore, a degassing device configured to pass the reaction tank water through a narrow pipe line passing through the vacuum vessel is provided in the circulation path of the reaction tank water so as to remove dissolved gas from the circulating reaction tank water. ing.

  However, the above prior art does not specifically describe monitoring of the deaeration state. Therefore, for example, if the deaeration becomes insufficient due to clogging of the pipe line or a decrease in the degree of vacuum due to the leakage of outside air into the vacuum vessel, the stability and accuracy of the measurement results are reduced as a result. There was a fear.

  The present invention has been made in view of the above, and by monitoring the deaeration state of the reaction tank water, it is possible to suppress a decrease in stability and accuracy of measurement results due to insufficient deaeration of dissolved air. An object of the present invention is to provide an automatic analyzer that can be used.

  To achieve the above object, the present invention provides a reaction vessel that contains a mixed solution of a sample and a reagent, a reaction vessel that holds a liquid that adjusts the temperature of the mixed solution by immersing the reaction vessel, A circulation line including a supply line and a discharge line that respectively supply and discharge the liquid in the reaction tank; a circulation pump that circulates the liquid in the circulation line; and a liquid that circulates through the circulation line. A temperature adjusting means for adjusting the temperature; a gas-liquid separation line provided in the liquid circulation line; a vacuum container provided so as to contain the gas-liquid separation line; and deaeration in the vacuum container The atmospheric pressure in the vacuum vessel is controlled by controlling the start and stop of the vacuum pump based on the detection result of the atmospheric pressure detector. To be in the normal pressure range set in advance Gyoshi, and that a control unit for notifying the operator to detect the abnormality of the dissolved gas content of the liquid on the basis of the operation period of the vacuum pump.

  According to the present invention, it is possible to suppress a decrease in stability and accuracy of measurement results due to insufficient degassing of the reaction tank water.

1 is a diagram schematically showing an overall configuration of an automatic analyzer according to an embodiment. FIG. It is the figure which extracts the reaction tank water circulation device of the automatic analyzer which relates to one execution form with the peripheral constitution, schematically. It is a figure which shows notionally an example of a structure of the vacuum vessel which concerns on one Embodiment. It is a figure which shows typically the atmospheric pressure change in the vacuum vessel at the time of the atmospheric | air pressure control process which concerns on one Embodiment, and the operation condition of a vacuum pump corresponding to a time axis. It is a flowchart which shows the process of the reference | standard period calculation process which concerns on one Embodiment. It is a flowchart which shows the deaeration abnormality detection process which concerns on one Embodiment. It is a figure which shows roughly the time change of the atmospheric | air pressure in a vacuum vessel at the time of an atmospheric | air pressure control process as contrasted with dissolved oxygen concentration. It is a figure which shows roughly the time change of the atmospheric | air pressure in a vacuum vessel at the time of an atmospheric | air pressure control process as contrasted with dissolved oxygen concentration. It is a flowchart which shows the process of the reference | standard period calculation process which concerns on the modification of one Embodiment.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings.

  FIG. 1 is a diagram schematically showing an overall configuration of an automatic analyzer according to the present embodiment. FIG. 2 is a diagram schematically showing the reaction tank water circulation device with its peripheral configuration extracted, and FIG. 3 is a diagram conceptually showing an example of the configuration of the vacuum vessel.

  In FIG. 1, the automatic analyzer includes a sample disk 5, first and second reagent disks 13A and 13B, reaction disk 1, sample dispensing mechanism 7, reagent dispensing mechanisms 12A and 12B, and other components including a computer 18. It is composed roughly of functional parts.

  In the sample disk 5, a plurality of sample containers 6 in which specimens to be analyzed (hereinafter referred to as samples) such as blood and urine are accommodated are arranged in the circumferential direction. The sample disk 5 is rotationally driven in the circumferential direction by a rotation drive mechanism (not shown), thereby moving the sample container 6 to a predetermined position.

  The first and second reagent disks 13A and 13B are respectively provided with reagent coolers 9A and 9B, and a plurality of reagent bottles 10A and 10B containing reagents used for each processing item of analysis processing in the automatic analyzer are provided. They are arranged side by side in the circumferential direction. The first and second reagent disks 13A and 13B are rotationally driven in a circumferential direction by a rotation driving mechanism (not shown), thereby moving the reagent bottles 10A and 10B to predetermined positions. The first and second reagent disks 13A and 13B are provided with reading devices 34A and 34B for reading the reagent identification information provided in the reagent bottles 10A and 10B. The position information on the second reagent disks 13A and 13B is sent to the computer 18 via the interface 19, and stored in the memory 11 in association with the measurement date and time. The reagent identification information is represented by, for example, a barcode, and the readers 34A and 34B are barcode readers.

  The reaction disk 1 includes a reaction tank 3 filled with reaction tank water 3a (for example, pure water) which is a temperature adjusting liquid adjusted to a temperature suitable for the reaction (for example, 37 ° C.) by the reaction tank water circulation device 4. In addition, a plurality of reaction vessels 2 in which a mixed solution (reaction solution) of a sample and a reagent is accommodated are arranged side by side in the circumferential direction in a state of being immersed in the reaction tank water 3a. The reaction disk 1 is rotated in the circumferential direction by a rotation drive mechanism (not shown), thereby moving the reaction container 2 to a predetermined position. The reaction tank 3 includes a discharge pipe 31 for discharging the reaction tank water 3 a in the reaction tank 3 to the reaction tank water circulation device 4, and a supply pipe 32 for supplying the reaction tank water 3 a from the reaction tank water circulation device 4. Is provided.

  As shown in FIG. 2, the reaction tank water circulation device 4 removes (degass) dissolved air (for example, dissolved oxygen) from the reaction tank water 3 a discharged through the discharge pipe 31 of the reaction tank 3. Direct cooling without passing through the vacuum degassing device 40 without the vacuum degassing device 40, and the cooling unit 45 that cools the reaction bath water passing through the vacuum degassing device 40. The bypass pipe 44 to be sent to the unit 45, the reaction tank water from the cooling unit 45 is sucked and discharged to the supply line 32 side of the reaction tank 3, and the reaction tank water from the circulation pump 46 is heated. And a heater 47 for supplying water to the reaction tank 3. The reaction tank 3, the discharge pipe 31, the supply pipe 32, and the reaction tank water circulation device 4 include a supply pipe 32 and a discharge pipe 31 that respectively supply and discharge liquid (reaction tank water) in the reaction tank. It constitutes a pipeline. The cooling unit 45, the heater 47, and the circulation pump 46 are controlled by the computer 18.

  The vacuum deaerator 40 includes a vacuum vessel 43, a vacuum pump 42 that draws air from the vacuum region 43b of the vacuum vessel 43 and makes a vacuum state, and an atmospheric pressure detector that is provided in the vacuum pump 42 and detects the atmospheric pressure in the vacuum region 43b The pressure control process (described later) for adjusting the output of the vacuum pump 42 (the exhaust force of air to the vacuum region 43b) based on the detection result of 42a, and the deaeration state of the reaction tank water circulating through the reaction tank circulation device 4 And a vacuum control unit 41 that performs a deaeration state detection process (described later) to be detected. The vacuum controller 41 is controlled by the computer 18 which is a higher-level control means. The vacuum controller 41 may be controlled by a built-in computer.

  As shown in FIG. 3, a gas-liquid separation membrane tube 43a formed by a gas-liquid separation membrane that allows gas to pass while blocking passage of liquid is passed through the vacuum region 43b inside the vacuum vessel 43. The reaction tank 3 is arranged to be sent to the cooling unit 45 side through the reaction tank water from the discharge pipe 31 of the reaction tank 3. By passing the reaction tank water (pure water) through the gas-liquid separation membrane tube 43a while the vacuum region 43b is in a vacuum state, only the air (oxygen) in the reaction tank water passes through the gas-liquid separation membrane and is vacuumed. It moves to the area | region 43b side and deaeration of reaction tank water is performed.

  In addition, the gas-liquid separation membrane which forms the gas-liquid separation membrane tube 43a in this Embodiment uses the thing which interrupts | blocks reaction tank water (pure water) as a liquid, and allows passage of oxygen (air) as a gas. However, it goes without saying that the gas-liquid separation membrane of the gas-liquid separation membrane tube 43a is appropriately selected according to the type of gas or liquid that is allowed or blocked.

  The bypass line 44 is arranged in parallel with the vacuum deaerator 40 in the circulation line, and has a variable throttle 44a that adjusts the passage amount of the reaction tank water. The ratio of the flow rate of the reaction tank water between the vacuum degassing device 40 and the bypass line 44 is adjusted by adjusting the flow rate by controlling the variable throttle 44 a of the bypass line 44 by the computer 18. It should be noted that instead of the variable throttle 44a, a simple throttle may be used, and the flow rate ratio may be adjusted by control by the computer 18.

  In the circulation line between the cooling unit 45 and the circulation pump 46, a drain line 48 for draining the reaction tank water from the circulation line to a drain tank (not shown), and pure water is generated as the reaction tank water. A water supply line 49 for supplying pure water generated by a pure water generator (not shown) and stored in the water supply tank 51 to the circulation line by the water supply pump 50 is connected. The drain pipe 48 is provided with an open / close valve 48 a, and the water supply pipe 49 is provided with an open / close valve 49 a, which is controlled to open and close by the computer 18.

  The sample dispensing mechanism 7 dispenses the sample accommodated in the sample container 6 into the reaction container 2, and the reagent dispensing mechanisms 12A and 12B include the reagent accommodated in the reagent bottles 10A and 10B in the reaction container 2. To dispense. The mixed solution (reaction solution) of the sample and the reagent dispensed into the reaction container 2 is stirred by the stirring mechanisms 33A and 33B provided at the respective dispensing positions in the reagent dispensing mechanisms 12A and 12B.

  The operations of the sample disk 1 and the sample dispensing mechanism 7 are controlled by the sample dispensing control unit 20. The operations of the first and second reagent disks 13A and 13B, the reagent dispensing mechanisms 12A and 12B, and the stirring mechanisms 33A and 33B are controlled by the reagent dispensing control unit 21. The sample dispensing control unit 20 and the reagent dispensing control unit 21 are controlled by a computer 18 connected via an interface 19.

  The reaction disk 1 includes a transmitted light amount measurement unit 35 that measures the transmitted light amount of a mixed solution (reaction solution) of a sample and a reagent accommodated in the reaction container 2. The transmitted light amount measuring unit 35 contains a mixed solution of a sample and a reagent and irradiates the reaction vessel 2 immersed in the reaction vessel water 3a of the reaction vessel 3 with multi-wavelength light (for example, a halogen light source). , And a transmitted light amount detector 15 for detecting the transmitted light amount of the transmitted light that passes through the reaction vessel 2 and the mixed liquid as the container. When the reaction vessel 2 driven in the reaction tank 3 in the circumferential direction of the reaction disk 1 passes between the multi-wavelength light source 14 and the transmitted light amount detector 15, the transmitted light amount is detected. The transmitted light amount (detection result) detected by the transmitted light amount detector 15 is digitally converted by the A / D converter 16 and sent to the computer 18 via the interface 19. The reaction container 2 in which the sample (mixed solution) for which the measurement has been completed is cleaned by the cleaning mechanism 17 at the cleaning position.

  The automatic analyzer includes a keyboard 24 as an input device, a CRT display 25 as a display device, a printer 22 as a print output device, a recording medium drive 23 for recording on an external output medium such as an FD, a storage device (storage unit) The memory 11 is connected to each functional unit including the computer 18 via the interface 19. The memory 11 is a storage device such as a hard disk, and stores information such as passwords set for each operator, screen display level, analysis parameters, analysis request item contents, calibration results, in addition to analysis results. . The memory 11 stores software, setting values, and the like used for atmospheric pressure control processing (described later) and deaeration state detection processing (described later).

  The computer 18 has a function of performing an atmospheric pressure control process (described later) and a deaeration state detection process (described later) in addition to a function as a control unit that controls the operation of the entire automatic analyzer. Qualitative / quantitative analysis of the target sample based on the command input, etc., as well as pressure control processing (described later) and degassing state detection processing (described later) to detect abnormalities in the degassing state and react The aim is to suppress the deterioration of the stability and accuracy of the measurement results due to insufficient degassing of the tank water.

  Here, the atmospheric pressure control process and the deaeration state detection process in the automatic analyzer according to the present embodiment will be described with reference to the drawings.

<Atmospheric pressure control processing>
The atmospheric pressure control process is a control for adjusting the atmospheric pressure in the vacuum vessel 43 to a preset atmospheric pressure range during normal operation.

  FIG. 4 is a diagram schematically showing a change in atmospheric pressure in the vacuum vessel and an operation state of the vacuum pump in association with the time axis during the atmospheric pressure control process in the present embodiment. In FIG. 4, the upper row shows whether the operating state of the vacuum pump 42 is operating (ON) or stopped (OFF), the lower row shows the detected value of the atmospheric pressure detector 42a, and the time (t) on the horizontal axis. Is shown.

  As shown in FIG. 4, the initial atmospheric pressure in the vacuum vessel 43 (that is, the atmospheric pressure in the vacuum region 43a) is atmospheric pressure. Here, when an instruction to start degassing of the thermostatic bath water (pure water) is given from the computer 18 or the like, which is the upper control means, the vacuum control unit 41 instructs the start of the operation of the vacuum pump 42 (time t0). ), The vacuum pump 40 is started, and the atmospheric pressure in the vacuum region 43a (that is, the detected value of the atmospheric pressure detector 42a) decreases. When the atmospheric pressure in the vacuum region 43a reaches the lower threshold value preset and stored in the memory 11, the vacuum pump 42 is stopped (time t1). As described above, the period from the atmospheric pressure to the lower threshold (time t0 to t1) is particularly referred to as initial pressure reduction. Thereafter, the vacuum pump 42 is stopped and waited. When the atmospheric pressure in the vacuum region 43a reaches the upper threshold value preset and stored in the memory 11, the vacuum pump 42 is started (time t2), and the atmospheric pressure in the vacuum region 43a is increased. When the lower threshold value is reached, the vacuum pump 42 is stopped (time t3). And the control similar to time t1-t3 is repeatedly performed (time t3-t5, t5-t7, ...). Thus, by controlling the atmospheric pressure in the vacuum region 43a to a predetermined atmospheric pressure range (that is, between the lower threshold value and the upper threshold value), the reaction tank water passing through the gas-liquid separation membrane tube 43a in the vacuum region 43a ( The deaerated state of pure water) is kept almost constant. Here, from the arrival of the lower threshold value of the detected value by the atmospheric pressure detector 42a until the lower threshold value is reached again with the arrival of the upper threshold value (time t1 to t3, t3 to t5, t5 to t7, etc.) 1 A period is defined as a period C (t).

<Deaeration state detection process>
In the deaeration state detection process, the deaeration state of the reaction tank water (pure water) by the vacuum deaeration device 40 is monitored, the abnormality is detected, and the operator is notified and the deaeration of the reaction tank water is insufficient. This is a process for suppressing a decrease in stability and accuracy of measurement results. The deaeration state detection process includes a reference period calculation process for calculating a reference period used for the process and a deaeration abnormality detection process for detecting an abnormality in the deaeration state using the reference period.

  FIG. 5 is a flowchart showing the process of the reference period calculation process in the present embodiment.

  When instructed to detect the deaeration state from the computer 18, the vacuum control unit 41 first reads a preset cycle measurement timing T and cycle measurement count N from the memory 11 (step S10). Next, as variables used for processing, variables S and S0 used for calculation of period C, variables t and t0 used for counting the number of measurements after initialization of period C, and variables used for counting the number of additions of period C n and n0 are initialized (numerical value 0 is substituted) (step S20). Thereafter, the first cycle C after the initial pressure reduction (see FIG. 4) is measured (step S30), and t0 + 1 is substituted into the variable t (step S40). Subsequently, it is determined whether or not the variable t is equal to or greater than the cycle measurement timing T (step S50). If the determination result is NO, t is substituted for the variable t0 (step S51), and the cycle C is initialized ( C = 0) (step S52), and the processing of steps S30 and S40 is repeated until the determination result is YES. If the decision result in the step S50 is YES, the cycle C is measured (step S60), n0 + 1 is substituted for the variable n (step S70), and S0 + C is substituted for the variable S (step S80). Subsequently, it is determined whether or not the variable n is equal to or greater than the number of period measurements N (step S90). If the determination result is NO, S is substituted for the variable S0 (step S91), and n is substituted for the variable n0. (Step S92), the period C is initialized (C = 0) (Step S93), and the processing of Steps S60 to S80 is repeated until the determination result is YES. If the determination result in step S90 is YES, the reference period X (= S / n) is calculated and determined, stored in the memory 11, and the process is terminated (step S100).

  Note that the period measurement timing T and the period measurement number N are determined experimentally. For example, the period measurement timing T is set in consideration of the time from the initial pressure reduction (the number of repetitions of the period) that the change period of the atmospheric pressure in the vacuum region 43a (that is, the detection value of the atmospheric pressure detector 42a) is sufficiently stabilized. doing. Further, the number N of period measurements is set to be large enough to ensure the reliability of the reference period X based on the average value of the period C.

  FIG. 6 is a flowchart showing the deaeration abnormality detection process in the present embodiment.

  When an instruction for the deaeration abnormality detection process is given from the computer 18, the vacuum control unit 41 first reads the reference period X calculated by the reference period calculation process from the memory 11 (step S110). Next, a predetermined alarm condition Z is read from the memory 11 (step S120), and the cycle C and variable C0 are initialized (step S130). Subsequently, the period C is measured (step S140), and it is determined whether C / X is equal to or higher than the alarm condition Z (step S150). If the determination result is NO, C is initialized (C = 0). (Step S151), and the process of Step S140 is repeated until the determination result is YES. Moreover, when the determination result in step S150 is YES, it determines with the deaeration state of reaction tank water being abnormal, alert | reports an alarm (step S160), and complete | finishes a process.

  The alarm condition Z is determined experimentally. For example, the deaeration state is abnormal from the ratio of the period C of the atmospheric pressure in the vacuum region 43a (that is, the detection value of the atmospheric pressure detector 42a) and the reference period X. It is set so large that it can be determined.

  The operational effects of the present embodiment configured as described above will be described with reference to the drawings.

  FIG. 7 and FIG. 8 are diagrams schematically showing a temporal change of the atmospheric pressure in the vacuum vessel 43a during the atmospheric pressure control process in comparison with the dissolved air concentration (here, dissolved oxygen concentration).

  7 and 8, for example, the period of atmospheric pressure change when the dissolved oxygen concentration is 5.3 [mg / L] is m0, and the period when the dissolved oxygen concentration is 6.5 [mg / L] is m1. The period when the dissolved oxygen concentration is 2.9 [mg / L] is shown as m2. The dissolved oxygen concentrations shown in FIGS. 7 and 8 are numerical values exemplified for explaining the magnitude relationship.

  As shown in FIG. 7, when the dissolved oxygen concentration is 6.5 [mg / L], when the dissolved oxygen concentration is 6.5 [mg / L], m1 represents the cycle when the dissolved oxygen concentration is 5.3 [mg / L]. Shorter. Further, as shown in FIG. 8, the period when the dissolved oxygen concentration is 2.9 [mg / L] is compared with the period m0 where the dissolved oxygen concentration of the reaction tank water is 5.3 [mg / L]. m2 becomes longer. That is, if the dissolved oxygen concentration in the reaction tank water is high, the cycle becomes shorter, and if the dissolved oxygen concentration is low, the cycle becomes longer. The present invention has been made on the basis of such knowledge, and the measurement was performed with the period when the dissolved oxygen concentration is a desired value (a value in a range determined to be normal) as a reference (reference period X). The abnormality of the deaeration state of the reaction tank water is detected from the relationship with the cycle (cycle C).

  The automatic analyzer in the prior art measures the optical characteristics while the reaction vessel containing the reaction solution is immersed in the reaction vessel water, and bubbles generated in the reaction vessel water make the measurement result stable and accurate. Since there is concern about adverse effects, a degassing device is provided in the reaction tank water circulation path so as to remove dissolved gas from the circulating reaction tank water. However, when using a degassing device configured to pass the reaction tank water through a narrow pipe line passing through the vacuum vessel as in the above prior art, the pipe line is clogged or external air leaks into the vacuum vessel. Due to the decrease in the degree of vacuum due to, degassing of the reaction vessel water becomes insufficient, and as a result, the stability and accuracy of the measurement results may be reduced.

  On the other hand, in the automatic analyzer in the present embodiment, by controlling the start and stop of the vacuum pump periodically, the pressure in the vacuum vessel is controlled within a predetermined pressure range, and the operation of the vacuum pump Since it is configured to detect an abnormality in the dissolved gas amount of the liquid based on the cycle and notify the operator, the stability and accuracy of the measurement result due to insufficient degassing of the reaction tank water is suppressed. can do.

  In the present embodiment, when the quotient (that is, C / X) in the predetermined reference period X of the operation period C of the vacuum pump 42 is equal to or greater than the predetermined threshold value Z, the reaction tank water is dissolved. Although it is configured to determine that the gas amount is abnormal, the present invention is not limited to this, and the reverse quotient (that is, X / C) may be determined to be equal to or less than a predetermined threshold. Therefore, it may be determined that there is an abnormality based on these ratios. Further, for example, when the operation cycle C (t) of the vacuum pump 42 is equal to or longer than a predetermined determination cycle (for example, Z0) (that is, when C ≧ Z0), the liquid dissolved gas amount is abnormal. You may comprise so that it may determine. As long as it can be determined that the operation cycle C (t) of the vacuum pump 42 is greater than the predetermined cycle, the determination value is not limited to these. In this case, the same effect as that of the present embodiment can be obtained.

  In addition, the relationship between the operation period C of the vacuum pump 42 and the dissolved air amount of the reaction tank water is experimentally calculated in advance, and the dissolved air amount is calculated from the operation period C and displayed on the CRT display 25. The operator can know the amount of dissolved air in the reaction tank water quantitatively, and can perform more accurate measurement.

  Further, when the dissolved air amount of the reaction tank water is determined to be abnormal, the circulation pump 46 may be driven in the reverse direction. When the amount of dissolved air in the reaction tank water is abnormal due to clogging of the gas-liquid separation membrane line 43a, the clogging of the gas-liquid separation line 43a is eliminated by driving the circulation pump 46 in the reverse direction for a certain time. Therefore, it is expected that the abnormality of dissolved air amount will be improved.

(Modification of this embodiment)
A modification of the present embodiment will be described with reference to the drawings.

  In this modification, in the reference cycle calculation process, the operation cycle of the vacuum pump 42 is repeatedly measured, and when the rate of change in the cycle falls below a predetermined range, the cycle is determined as the reference cycle X. .

  FIG. 9 is a flowchart showing the reference cycle calculation process in the present modification. In the figure, members similar to those in the present embodiment are denoted by the same reference numerals, and description thereof is omitted.

  When the vacuum control unit 41 of the present modification is instructed to perform the deaeration state detection process from the computer 18, first, the preset cycle determination value Y is read from the memory 11 (step S210). Next, initialization of periods C and C0 (substitution of numerical value 0) is performed as variables used in the processing (step S220). Thereafter, the first cycle C after the initial pressure reduction (see FIG. 4) is measured (step S230), and the cycle C is substituted into the cycle variable C0 (step S240). Subsequently, the period C is initialized (C = 0) (step S250), the period C is measured (step S260), and it is determined whether the period C / the period C0 is equal to or less than the period determination value Y (step S260). Step S270). When the determination result in step S270 is NO, the processes in steps S240 to S260 are repeated until the determination result is YES. If the decision result in the step S270 is YES, the period C at that time is determined as the reference period X, stored in the memory 11, and the process is ended (step S280).

  Other configurations are the same as those of the present embodiment.

  Also in this modified example configured as described above, the same effect as in the present embodiment can be obtained.

DESCRIPTION OF SYMBOLS 1 Reaction disk 2 Reaction container 3 Reaction tank 4 Reaction tank water circulation apparatus 5 Sample disk 7 Sample dispensing mechanism 9A, 9B Reagent cooler 10A, 10B Reagent bottle 11 Memory 12A, 12B Reagent dispensing mechanism 13A 1st reagent disk 13B 2nd Reagent disc 14 Single wavelength light source 15 Light scattering light quantity detector 16 A / D converter 17 Cleaning mechanism 18 Computer 19 Interface 20 Sample dispensing control unit 21 Reagent dispensing control unit 22 Printer 23 Recording medium drive 24 Keyboard 25 CRT display 33A, 33B Agitation mechanism 34A, 34B Reading device 35 Light scattering light quantity measurement unit 40 Vacuum deaeration device 41 Vacuum control unit (control unit)
42 Vacuum pump 42a Barometric pressure detector 43 Deaeration vessel 44 Bypass conduit 45 Cooling unit 46 Circulation pump 47 Heater 48 Drain conduit 49 Water supply conduit 48a, 49a Open / close valve 50 Water supply pump 51 Water supply tank

Claims (5)

A reaction vessel containing a mixture of the sample and the reagent;
A reaction vessel for holding a liquid for immersing the reaction vessel to adjust the temperature of the mixed solution;
A circulation line including a supply line and a discharge line that respectively supply and discharge the liquid in the reaction tank;
A circulation pump for circulating the liquid in the circulation line;
Temperature adjusting means for adjusting the temperature of the liquid circulating in the circulation line;
A gas-liquid separation line provided in the liquid circulation line;
A vacuum vessel provided to contain the gas-liquid separation conduit;
A vacuum pump for degassing the vacuum vessel;
An atmospheric pressure detector for detecting the atmospheric pressure in the vacuum vessel;
By controlling the start and stop of the vacuum pump based on the detection result of the atmospheric pressure detector, the atmospheric pressure in the vacuum vessel is controlled to be within a predetermined normal atmospheric pressure range, and based on the operation cycle of the vacuum pump And a controller for detecting an abnormality in the dissolved gas amount of the liquid and notifying the operator. The automatic analyzer according to claim 1, wherein
The control unit controls the vacuum pump to stop when the detection result of the atmospheric pressure detector reaches the lower limit of the normal atmospheric pressure range, and to operate the vacuum pump when the detection result reaches the upper limit. An automatic analyzer. The automatic analyzer according to claim 1, wherein
The said analysis part reversely drives the said circulation pump, when the abnormality of the dissolved gas amount of the said liquid is detected, The automatic analyzer characterized by the above-mentioned. The automatic analyzer according to claim 1 or 2,
Wherein the control unit, based on said ratio of the operation period (t) with a predetermined reference period of the vacuum pump, an automatic analyzer and judging that there is an abnormality of the dissolved gas content of the liquid. The automatic analyzer according to claim 1 or 2,
The said control part determines with it being abnormal of the dissolved gas amount of the said liquid, when the operation period (t) of the said vacuum pump exceeds the predetermined determination period, The automatic analyzer characterized by the above-mentioned.

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