JP5971690B2 - Freezing method of biological material - Google Patents
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Description
本発明は、生体物質の凍結方法に関するものである。 The present invention relates to a biological material freezing method.
食品や、細胞および卵等の生体試料などの生体物質を凍結する際に、それらの生体物質の損傷を防ぐために凍害保護剤が用いられている。その凍害保護剤としては、細胞外で作用するものとして、トレハロースやスクロースなどがあり、細胞内で作用するものとして、グリセロールやジメチルスルホオキシド（ＤＭＳＯ）などがある。また、近年では不凍タンパク質（ＡＦＰ）の凍害保護機能についても注目されてきている。 When freezing biological materials such as food and biological samples such as cells and eggs, frost damage protective agents are used to prevent damage to those biological materials. As the frost damage protective agent, there are trehalose and sucrose that act extracellularly, and glycerol and dimethyl sulfoxide (DMSO) and the like that act intracellularly. In recent years, attention has also been paid to the frost damage protection function of antifreeze protein (AFP).
これら凍害保護剤は、一定の濃度の水溶液である凍害保護液として利用されており、細胞および卵等の凍結保存においては、培養液中に添加する形で使用されている（例えば、特許文献１参照）。ＡＦＰについては、食品の改質機能についても検討が進められている（例えば、特許文献２参照）。 These frost damage protective agents are used as a frost damage protection solution that is an aqueous solution having a constant concentration, and are used in the form of being added to a culture solution in cryopreservation of cells and eggs (for example, Patent Document 1). reference). As for AFP, studies are also being made on a food reforming function (see, for example, Patent Document 2).
図５は、凍害保護剤を使用した従来の生体物質の凍結方法における課題を説明するための図である。すなわち、単一の細胞や卵の場合は、凍害保護液中、あるいは凍害保護液を添加した培養液中において直接の作用が可能である。 FIG. 5 is a diagram for explaining a problem in a conventional biological material freezing method using a frost damage protective agent. That is, in the case of a single cell or egg, a direct action is possible in the frost damage protection solution or in the culture solution to which the frost damage protection solution is added.
しかしながら、図５に示すように、凍結対象物１がウニ生殖腺のような魚介類の生殖腺または卵塊の場合には、細胞あるいは卵粒１１の大きな集合体であるため、それらを塊（かたまり）として凍害保護液中に浸漬するのみでは、凍害保護液はその塊の表面部分にのみ浸透するだけで、その内部には浸透しにくい。従って、凍害保護剤の効果は、その塊の表面部分の各細胞または卵粒１１に表れるだけであり、その深い内部の各細胞または卵粒１１には表れにくい。かかる場合は、塊の分断が許される場合はいいが、それが不可能な場合も多く、その場合には、その塊のままで、その内部にも凍害保護剤の効果が行き渡るような方策が望まれている。 However, as shown in FIG. 5, when the frozen object 1 is a gonad or egg mass of a seafood such as a sea urchin gonad, it is a large aggregate of cells or egg grains 11. By simply immersing in the frost damage protection liquid, the frost damage protection liquid only penetrates into the surface portion of the lump and hardly penetrates into the inside. Therefore, the effect of the frost damage protective agent only appears on each cell or egg particle 11 on the surface portion of the lump, and hardly appears on each deep cell or egg particle 11. In such a case, it is okay if fragmentation is allowed, but there are many cases where this is not possible, and in that case, there is a measure to keep the effect of the frost damage protective agent inside the mass. It is desired.
なお、例えば特許文献３が、マイクロ波減圧脱水技術について開示しているが、それは、生体物質の凍結に係る技術とは全く異なる分野の技術である。 For example, Patent Document 3 discloses a microwave vacuum dehydration technique, which is a technique in a field completely different from the technique related to freezing of biological materials.
本発明は上述のような事情から為されたものであり、本発明の目的は、解凍後に、凍害保護剤の効果がより顕れるような生体物質の凍結方法を提供することにある。 The present invention has been made in view of the above circumstances, and an object of the present invention is to provide a method for freezing a biological material such that the effect of a frost damage protective agent becomes more apparent after thawing.
本発明は、上記事情に鑑みてなされたものであって、解凍後に、凍害保護剤の効果がより顕れるような生体物質の凍結方法を提供することを目的とする。 This invention is made | formed in view of the said situation, Comprising: It aims at providing the freezing method of the biological material which the effect of a frost damage protective agent becomes more evident after thawing | decompression.
請求項１に係る発明は、脱水率が凍結対象物である生体物質の重量の３〜５０％となるように、マイクロ波減圧方式によって当該生体物質の内部まで脱水処理し、
脱水処理後の生体物質を凍害保護液に浸漬処理し、
浸漬処理後の生体物質を凍結することを特徴とする生体物質の凍結方法である。
The invention according to claim 1, as dehydration rate is 3-50% by weight of the biological material is frozen object, dehydrated until the interior of the biological material by microwave vacuum system,
The biological material was dehydrated and dipped in cryoprotective solution,
The biological material freezing method is characterized in that the biological material after the immersion treatment is frozen.
請求項３に係る発明は、前記浸漬処理後、前記凍結処理前に、前記生体物質に対して第２の脱水処理を行うことを特徴とする請求項１に記載の生体物質の凍結方法である。 The invention according to claim 3 is the biological material freezing method according to claim 1, wherein the biological material is subjected to a second dehydration treatment after the immersion treatment and before the freezing treatment. .
請求項３に係る発明は、前記第２の脱水処理における脱水率を前記脱水処理における脱水率より低くすることを特徴とする請求項２に記載の生体物質の凍結方法である。 The invention according to claim 3 is the freezing method of the biological material according to claim 2, characterized in that the dewatering rate in the second dehydration treatment lower than dewatering rate in the dehydration process.
請求項４に係る発明は、前記生体物質は、魚介類の生殖腺または卵塊であることを特徴とする請求項１乃至３のいずれかに記載の生体物質の凍結方法である。 The invention according to claim 4 is the biological material freezing method according to any one of claims 1 to 3 , wherein the biological material is a gonad or egg mass of a seafood.
本発明の生体物質の凍結方法によれば、解凍後に、凍害保護液の効果がより顕れる。つまり、凍害保護液への浸漬処理前に生体物質に対して脱水処理を行うので、浸漬中に凍害保護液が生体物質の内部にまで均一に浸透し、凍害保護液の凍害保護機能が生体物質の内部において十分に発揮され、凍結・解凍後の品質劣化をより抑えることができる。また、浸漬処理後、凍結処理前に、第２の脱水処理を行えばより効果的である。ここで、脱水処理における脱水率を所定の範囲で行えば、上述の効果は確実に発揮される。 According to the method for freezing biological material of the present invention, the effect of the frost damage protection liquid is more apparent after thawing. In other words, since the biological material is dehydrated before being immersed in the frost damage protection liquid, the frost damage protection liquid penetrates uniformly into the biological material during the immersion, and the frost damage protection function of the frost damage protection liquid is the biological material. It can be fully exerted in the interior of the container, and quality deterioration after freezing and thawing can be further suppressed. It is more effective if the second dehydration process is performed after the immersion process and before the freezing process. Here, if the dehydration rate in the dehydration process is performed within a predetermined range, the above-described effects are surely exhibited.
また、脱水処理をマイクロ波減圧方式により行えば、生体物質に損傷を与えることなく脱水することができる。
特に、本発明は、生体物質が魚介類の生殖腺または卵塊のような場合にも確実に脱水され、より有効となる。
Further, if the dehydration treatment is performed by a microwave decompression method, dehydration can be performed without damaging the biological material.
In particular, the present invention is surely dehydrated and more effective when the biological material is a gonad or egg mass of a seafood.
以下、本発明を適用した一実施形態である生体物質の凍結方法について、図面を用いて詳細に説明する。
なお、以下の説明で用いる図面は、特徴をわかりやすくするために、便宜上特徴となる部分を拡大して示している場合があり、各構成要素の寸法比率などが実際と同じであるとは限らない。
Hereinafter, a biological material freezing method according to an embodiment to which the present invention is applied will be described in detail with reference to the drawings.
In addition, in the drawings used in the following description, in order to make the features easy to understand, there are cases where the portions that become the features are enlarged for the sake of convenience, and the dimensional ratios of the respective components are not always the same as the actual ones. Absent.
図１は、本発明の生体物質の凍結方法の一実施形態の手順を示すフローチャートである。図２は、その手順を模式的に説明するための図である。図３は、その手順の過程中で使用されるマイクロ波減圧脱水装置の一例の構成図である。以下、図１乃至図３を参照して、本発明の生体物質の凍結方法の一実施形態の手順を説明する。なお、本発明においては、生体試料として魚介類の生殖腺または卵塊、好ましくはウニ生殖腺を凍結対象物とすることができるが、他の食品を凍結対象物としても構わない。 FIG. 1 is a flowchart showing a procedure of an embodiment of the biological material freezing method of the present invention. FIG. 2 is a diagram for schematically explaining the procedure. FIG. 3 is a configuration diagram of an example of a microwave vacuum dehydration apparatus used during the procedure. Hereinafter, with reference to FIG. 1 thru | or FIG. 3, the procedure of one Embodiment of the freezing method of the biological material of this invention is demonstrated. In the present invention, the gonads or egg masses of seafood, preferably sea urchin gonads, can be used as the biological object, but other foods may be used as the frozen object.
図２の左図に示すように、凍害保護液への浸透前の生体試料１は、水分を含有している。そこで、本発明の生体物質の凍結方法の一実施形態においては、まず、図３に示したマイクロ波減圧脱水装置２を使用して、生体試料１から、図２の中図に示すように、マイクロ波減圧脱水により、その内部に至るまで脱水する（図１のステップＳ１）。 As shown in the left diagram of FIG. 2, the biological sample 1 before penetration into the frost damage protection liquid contains moisture. Therefore, in one embodiment of the biological material freezing method of the present invention, first, using the microwave vacuum dehydration apparatus 2 shown in FIG. 3, from the biological sample 1, as shown in the middle diagram of FIG. Dehydration is performed up to the inside by microwave dehydration (step S1 in FIG. 1).
ここで、図３を参照して、マイクロ波減圧脱水装置２の一例の構成と、当該装置を用いた脱水処理の概要について説明する。 Here, with reference to FIG. 3, the structure of an example of the microwave pressure reduction dehydration apparatus 2 and the outline | summary of the dehydration process using the said apparatus are demonstrated.
図３に示すように、マイクロ波減圧脱水装置２は、真空チャンバー２１と、その真空チャンバー２１内に配設され、凍結対象物としての生体試料１が載置されるトレイ２２と、真空チャンバー２１内の生体試料１に照射するためのマイクロ波を発生させるマイクロ波発振器２３と、そのマイクロ波発振器２３の出力を調整するためのマイクロ波出力調整器２４と、生体試料１の表面および内部の温度を測定するための、例えば蛍光式光ファイバ式温度計等の温度センサー２５と、その温度センサー２５の測定値に基づき、マイクロ波出力調整器２４のマイクロ波発振器２３への出力調整信号の大きさを制御するための温度調節計２６と、真空チャンバー２１内を所定の真空状態に保つための真空ポンプ２７と、真空チャンバー２１内の真空状態（圧力）を測定するための真空計２８とを備えている。 As shown in FIG. 3, the microwave vacuum dehydration apparatus 2 includes a vacuum chamber 21, a tray 22 that is disposed in the vacuum chamber 21 and on which a biological sample 1 as an object to be frozen is placed, and a vacuum chamber 21. A microwave oscillator 23 for generating a microwave for irradiating the biological sample 1 inside, a microwave output regulator 24 for adjusting the output of the microwave oscillator 23, and the temperature of the surface and the inside of the biological sample 1 For example, a temperature sensor 25 such as a fluorescent optical fiber thermometer, and the magnitude of an output adjustment signal to the microwave oscillator 23 of the microwave output adjuster 24 based on the measured value of the temperature sensor 25 A temperature controller 26 for controlling the pressure, a vacuum pump 27 for maintaining the inside of the vacuum chamber 21 in a predetermined vacuum state, and a vacuum state in the vacuum chamber 21 And a vacuum gauge 28 for measuring the (pressure).
そこで、生体試料１に対する脱水作業の手順としては、まず、その生体試料１を真空チャンバー２１内のトレイ２２上に載置する。そして、マイクロ波発振器２３により出力１０Ｗ〜１５００Ｗの範囲内のマイクロ波を生成して、トレイ２２上の生体試料１に照射を開始する。マイクロ波の照射により温度が上昇するので、それと並行して、温度センサー２５により生体試料１の表面および内部の温度を測定する。そして、温度調節計２６は、生体試料１の表面および内部の温度が所定の範囲に収まっているように、マイクロ波出力調整器２４のマイクロ波発振器２３への出力調整信号を制御してマイクロ波出力のオンオフ制御を行う。 Therefore, as a procedure for dehydrating the biological sample 1, first, the biological sample 1 is placed on the tray 22 in the vacuum chamber 21. And the microwave within the range of output 10W-1500W is produced | generated by the microwave oscillator 23, and irradiation to the biological sample 1 on the tray 22 is started. Since the temperature rises due to the microwave irradiation, the temperature of the surface and the inside of the biological sample 1 are measured by the temperature sensor 25 in parallel therewith. Then, the temperature controller 26 controls the output adjustment signal to the microwave oscillator 23 of the microwave output adjuster 24 so that the temperature of the surface of the biological sample 1 and the internal temperature are within a predetermined range. Performs output on / off control.
なお、後述の好適脱水率から、それに対応するマイクロ波出力および照射時間が分かっているのであれば、上述のようにマイクロ波の照射をオンオフ制御するのではなく、一定時間の連続照射で対応することも可能である。 In addition, if the microwave output and irradiation time corresponding to the dehydration rate described later are known, the microwave irradiation is not controlled on and off as described above, but it is supported by continuous irradiation for a certain period of time. It is also possible.
なお、このマイクロ波照射の間、真空チャンバー２１内の圧力を減圧すべく、真空ポンプ２７を作動させる。そして、真空計２８の表示に基づき、真空チャンバー２１内の圧力を１ｋＰａ〜１００ｋＰａの圧力範囲の所望値に維持する。 During this microwave irradiation, the vacuum pump 27 is operated to reduce the pressure in the vacuum chamber 21. And based on the display of the vacuum gauge 28, the pressure in the vacuum chamber 21 is maintained at a desired value in a pressure range of 1 kPa to 100 kPa.
なお、上述の処理動作による脱水率は、細胞あるいは組織に対して損傷を与えない程度の量、具体的には、凍結対象物（ここでは生体試料１）の重量比で３〜５０％が好適である。また、全体の処理時間としては、１時間以内が好ましい。 It should be noted that the dehydration rate by the above processing operation is preferably an amount that does not damage cells or tissues, specifically, 3 to 50% in terms of the weight ratio of the object to be frozen (biological sample 1 here). It is. Further, the total processing time is preferably within 1 hour.
ここで、本明細書における「脱水率」とは、次式（１）により得られた値をいう。
脱水率（％）＝［（脱水処理前凍結対象物重量）−（脱水処理後凍結対象物重量）］／（脱水処理前凍結対象物重量）×１００ ・・・（１）
Here, “dehydration rate” in the present specification refers to a value obtained by the following equation (1).
Dehydration rate (%) = [(weight of object to be frozen before dehydration) − (weight of object to be frozen after dehydration)] / (weight of object to be frozen before dehydration) × 100 (1)
上述のような手順で脱水処理が終了すると、次に、図１に示すように、凍害保護液浸漬処理を行う（ステップＳ２）。つまり、脱水処理終了後、マイクロ波減圧脱水装置２の真空チャンバー２１から取り出した生体試料１を、凍害保護液に浸漬させる。 When the dehydration process is completed by the procedure as described above, a frost damage protection liquid immersion process is then performed as shown in FIG. 1 (step S2). That is, after completion of the dehydration treatment, the biological sample 1 taken out from the vacuum chamber 21 of the microwave vacuum dehydration apparatus 2 is immersed in the frost damage protection liquid.
なお、凍害保護液とは、凍害保護剤が所定の濃度溶解された水溶液のことをいう。また、その凍害保護剤とは、凍害保護機能が認識されている物質をいう。ここで、例えば、凍害保護剤は、トレハロース、スクロース、ＤＭＳＯ、不凍タンパク質であり、その凍害保護液濃度は、１００ｐｐｍ〜３０％であり、温度は常温以下であり、浸漬時間は５分〜１時間の範囲内である。 The frost damage protection liquid refers to an aqueous solution in which a frost damage protection agent is dissolved at a predetermined concentration. Further, the frost damage protective agent refers to a substance that has been recognized for its frost damage protection function. Here, for example, the frost damage protective agent is trehalose, sucrose, DMSO, antifreeze protein, the concentration of the frost damage protection liquid is 100 ppm to 30%, the temperature is room temperature or less, and the immersion time is 5 minutes to 1 Within time.
上述のような浸漬処理の結果、図２の右図に示すように、生体試料１の内部まで十分に均等に凍害保護液が染み渡る。 As a result of the immersion treatment as described above, as shown in the right diagram in FIG.
上述のような手順で十分な浸漬処理が終了すると、最後に、図１に示すように、冷凍装置により凍結処理を行う（ステップＳ３）。冷凍装置としては、生体試料１を冷凍可能な任意の装置を利用できる。なお、例えば、冷凍温度は、−２０〜−１９６℃の範囲内であり、冷凍時間は、１５秒〜１時間の範囲内である。 When the sufficient immersion process is completed by the procedure as described above, finally, as shown in FIG. 1, a freezing process is performed by a refrigeration apparatus (step S3). As the refrigeration apparatus, any apparatus that can freeze the biological sample 1 can be used. For example, the freezing temperature is in the range of −20 to −196 ° C., and the freezing time is in the range of 15 seconds to 1 hour.
なお、上述のステップＳ２の凍害保護液浸漬処理と、ステップＳ３の凍結処理の間に、第２の脱水処理を加えるとさらに好適である。但し、外観に影響を与える場合がある（食品、特に魚介類の生殖腺または卵塊については重要事項）ので、その脱水率は第１の脱水処理より低い脱水率とすることが好ましい。 It is more preferable to add a second dehydration process between the frost damage protection liquid immersion process in step S2 and the freeze process in step S3. However, since it may affect the appearance (important matter for food, particularly gonads or egg masses of seafood), the dehydration rate is preferably lower than that of the first dehydration treatment.
以上に説明した本発明の実施形態によれば、凍害保護液への浸漬前に、凍結対象物である生体試料１に対して脱水処理を行うので、浸漬中に、凍害保護液が、生体試料１の内部にまで均一に浸透する。従って、従来において凍結対象物の表層においてのみ効果のあった凍害保護機能が、凍結対象物全体に効果をもたらすため、凍結・解凍後の品質劣化をより抑えることができる。 According to the embodiment of the present invention described above, the dehydration treatment is performed on the biological sample 1 that is the object to be frozen before being immersed in the frost damage protection liquid. It penetrates evenly into the inside of 1. Therefore, since the frost damage protection function that has been effective only on the surface layer of the object to be frozen in the past has an effect on the entire object to be frozen, quality deterioration after freezing and thawing can be further suppressed.
また、特に食品に関し、脱水を行わない従来と比較して、解凍後の外観（色の変色など）が良好であり、かつ食感についても、凍結前の新鮮さが維持される。さらに、凍害保護液が生体試料１の内部にまで均一に浸透するので、従来行われていた、凍害保護液の高濃度化という方策も不必要となる。特に、脱水方式として、マイクロ波減圧脱水を採用すれば、生体試料１を損傷することなく脱水処理を行うことができる。また、脱水率も調整できる。 In addition, particularly for foods, the appearance after thawing (color discoloration, etc.) is good compared to the conventional case where dehydration is not performed, and the freshness before freezing is also maintained for the texture. Furthermore, since the frost damage protection liquid penetrates uniformly into the inside of the biological sample 1, a measure for increasing the concentration of the frost damage protection liquid, which has been conventionally performed, is also unnecessary. In particular, if microwave vacuum dehydration is employed as the dehydration method, dehydration can be performed without damaging the biological sample 1. Also, the dehydration rate can be adjusted.
以下に、具体的な実施例を示す。
（実施例１）
産卵前のキタムラサキウニを凍結対象物として、凍害保護剤の効果確認に関する比較試験を実施した。実際に使用した凍害保護剤、装置等、また、処理条件等を以下に示す。
Specific examples are shown below.
Example 1
A comparative test for confirming the effect of a frost damage protective agent was carried out on the frozen sea urchins before laying eggs. The actual frost damage protective agents, devices, etc., and treatment conditions are shown below.
調整液 ：３％（Ｗ／Ｗ）人工海水
凍害保護剤 ：植物由来不凍タンパク質
凍害保護液 ：凍害保護剤１００ｐｐｍ水溶液（調整液に溶解）
水切り ：キッチンペーパー上にて表面ラップフィルム後、＋５℃冷蔵庫静置。
脱水 ：マイクロ波減圧脱水装置にて、３ｋＰａにて減圧脱水。脱水中の対象物温度は常温以下。マイクロ波出力１００Ｗ、処理時間２０分。
凍結 ：液化窒素式冷凍装置にて凍結。温度−１２０℃、時間１０分。
解凍 ：密閉シャーレ内、ろ紙（アドバンテック社製５Ｃ）上にて静置。温度＋５℃。
Conditioning liquid: 3% (W / W) artificial seawater Frost damage protection agent: Plant-derived antifreeze protein Frost damage protection liquid: Frost damage protection agent 100ppm aqueous solution
Draining: After surface wrap film on kitchen paper, leave at + 5 ° C refrigerator.
Dehydration: Dehydration under reduced pressure at 3 kPa using a microwave vacuum dehydration apparatus. The object temperature during dehydration is below room temperature. Microwave output 100W, processing time 20 minutes.
Freezing: Freezing with a liquefied nitrogen type freezer. Temperature -120 ° C, time 10 minutes.
Thawing: Leave on a filter paper (5C manufactured by Advantech) in a sealed petri dish. Temperature + 5 ° C.
また、この実施例では、凍結対象物として、４つのサンプルを用意し、表１の流れに示すように、それぞれ異なる条件で試験を行い、効果を比較検証した。 In this example, four samples were prepared as objects to be frozen, and tests were performed under different conditions as shown in the flow of Table 1, and the effects were compared and verified.
すなわち、サンプル［１］は、手順＜凍結→解凍＞のサンプルであり、サンプル［２］は、手順＜凍害保護液浸漬→凍結→解凍＞のサンプルであり、サンプル［３］は、手順＜脱水→凍害保護液浸漬→凍結→解凍＞のサンプルであり、サンプル［４］は、手順＜脱水→凍害保護液浸漬→脱水→凍結→解凍＞のサンプルである。 That is, sample [1] is a sample of procedure <freezing → thawing>, sample [2] is a sample of procedure <immersion of frost damage protection solution → freezing → thawing>, and sample [3] is procedure <dehydration> → Sample of frost damage protection liquid immersion → freezing → thawing> Sample [4] is a sample of procedure <dehydration → frost damage protection liquid immersion → dehydration → freezing → thawing>.
なお、各サンプルに共通の最初の「調整液浸漬」処理は、サンプルの初期条件を揃えることを目的としたものである。 Note that the first “adjustment liquid immersion” process common to the samples is intended to align the initial conditions of the samples.
表１に示す処理の後、各サンプルについて、品質比較評価を行った。なお、ここではその品質評価指標として、冷凍食品の品質評価指標として一般的に用いられているドリップの量を採用した。つまり、各サンプルについて、凍結前重量と解凍後重量の測定結果より、下記式（１）に基づき、ドリップ率を算出した。
ドリップ率＝（凍結前重量−解凍後重量）／凍結前重量 ・・・（１）
After the processing shown in Table 1, quality comparison evaluation was performed for each sample. Here, as the quality evaluation index, the amount of drip generally used as a quality evaluation index for frozen foods was adopted. That is, for each sample, the drip rate was calculated based on the following formula (1) from the measurement results of the weight before freezing and the weight after thawing.
Drip rate = (weight before freezing−weight after thawing) / weight before freezing (1)
その結果、各サンプルについてのドリップ率は、表２に示すように求められた。 As a result, the drip rate for each sample was determined as shown in Table 2.
表２に示す結果により、凍害保護液浸漬前にマイクロ波減圧脱水したサンプルは、凍害保護機能をより効果的に発揮することが確認された。 From the results shown in Table 2, it was confirmed that the sample subjected to microwave dehydration before immersing the frost damage protection liquid exhibits the frost damage protection function more effectively.
また、解凍後の各サンプルの外観を図４に示した。同図に示すように、外観上でも、マイクロ波減圧脱水したサンプルであるサンプル［３］は、他のサンプルに比べて成分の溶出がなく、新鮮さが保持されていることがわかる。また、サンプル［４］のように、凍害保護液浸漬後にさらに脱水すると、ドリップはさらに低減される。この場合、色が濃くなる傾向が見られるが、２回目の脱水率を１回目の脱水処理における脱水率より低くし、例えば５％程度とするなど調整すればよい。 The appearance of each sample after thawing is shown in FIG. As shown in the figure, it can be seen that sample [3], which is a sample subjected to microwave dehydration under reduced pressure, has no elution of components and retains freshness compared to other samples. Further, as in sample [4], when the water is further dehydrated after being immersed in the frost damage protection liquid, the drip is further reduced. In this case, the color tends to be dark, but the second dehydration rate may be adjusted to be lower than the dehydration rate in the first dehydration process, for example, about 5%.
本発明の生体物質の凍結方法は、食品や、卵および細胞等の生体試料を凍結保存し、後に解凍して回復させる場合に適用することができる。 The biological material freezing method of the present invention can be applied to a case where food or biological samples such as eggs and cells are cryopreserved and later thawed for recovery.
１・・・生体試料（生体物質）
１１・・・細胞または卵粒
２・・・マイクロ波減圧脱水装置
２１・・・真空チャンバー
２２・・・トレイ
２３・・・マイクロ波発振器
２４・・・マイクロ波出力調整器
２５・・・温度センサー
２６・・・温度調節計
２７・・・真空ポンプ
２８・・・真空計
1 ... Biological sample (biological material)
DESCRIPTION OF SYMBOLS 11 ... Cell or egg 2 ... Microwave decompression dehydration device 21 ... Vacuum chamber 22 ... Tray 23 ... Microwave oscillator 24 ... Microwave output regulator 25 ... Temperature sensor 26 ... Temperature controller 27 ... Vacuum pump 28 ... Vacuum gauge
Claims (4)
脱水処理後の生体物質を凍害保護液に浸漬処理し、
浸漬処理後の生体物質を凍結することを特徴とする生体物質の凍結方法。 As dehydration rate is 3-50% by weight of the biological material is frozen object, dehydrated until the interior of the biological material by microwave vacuum system,
The biological material was dehydrated and dipped in cryoprotective solution,
A method for freezing a biological material, comprising freezing the biological material after the immersion treatment.
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