JP6029013B2 - Plant growth promoter and method for producing plant growth promoter - Google Patents

Description

  The present invention relates to a plant growth promoter.

  In recent years, the dangers of chemically synthesized pesticides, residual pesticides and the like have become problems, and pesticide-free cultivation, reduced pesticide cultivation, and the like are being actively promoted. Under such circumstances, development of a product called “plant vitality agent” with high safety is underway. There are many products that use herbal extracts to enhance safety. For example, Patent Document 1 discloses a plant growth promoter using a combination of a specific vitamin and glycyrrhizin, a herbal medicine component contained in a Glycyrrhiza plant.

Licorice plants are perennials of the leguminous family (Leguminosae) and grow primarily in dry areas such as northern China, southern Russia, Central Asia and the Mediterranean. For example, larva (G. uralensis) that grows naturally in northern China, and Spanish licorice (G. glabra) that grows widely in the Mediterranean region are well known. In the field of oriental medicine (Chinese medicine), dried roots containing strons of the licorice plant have long been used as the herbal medicine “licorice”. Licorice dry powder / extract has various pharmacological activities such as anti-inflammatory, anti-ulcer, anti-allergic, antitussive, anticancer, antiviral and antibacterial, and is also important as a pharmaceutical. Acid (C ₄₂ H ₆₂ O ₁₆ ) is included. In licorice plants, glycyrrhizic acid accumulates as a mixture of potassium and calcium salts, which are collectively referred to as glycyrrhizin. Glycyrrhizic acid, which is the main medicinal component of licorice, is one of the triterpene glycosides. It is contained in the roots of licorice plants and is extracted and purified from the roots.

  Wild licorice plants are harvested and used as a raw material for producing glycyrrhizic acid. On the other hand, problems of environmental destruction and resource depletion due to over-exploitation of licorice plants have become apparent.

JP-A-8-143406

  Since the plant growth promoter disclosed in Patent Document 1 uses glycyrrhizin contained in a licorice plant, a large amount of stable glycyrrhizic acid is required to produce this plant growth promoter. In addition, when only glycyrrhizin is used without being used in combination with a specific vitamin, the plant growth promoting effect is rather reduced. For this reason, the new plant growth promoter which can be supplied stably and has the outstanding growth promotion effect | action with respect to a plant is calculated | required.

  The present invention has been made in view of the above circumstances, and an object thereof is to provide a plant growth promoter that can be stably supplied and has an excellent growth promoting action on plants.

  In order to achieve the above object, the plant growth promoter according to the first aspect of the present invention comprises a component contained in the stem of a licorice plant strain having any one of SEQ ID NOs: 1 to 9 in the Sequence Listing. It is characterized by containing.

  The sequences are as follows: Uralanthus Gu2-2-1 strain, Gu2-3-2 strain, GuTS71-08IV1 (hereinafter referred to as “GuIV1”) strain, GuTS71-08IV2 (hereinafter referred to as “GuIV2”) strain, Alternatively, it may be a partial sequence of SQS (squalene synthase) 2 gene, CYP88D6 gene or CYP72A154 gene in strain identification number 11 (hereinafter referred to as “Gu # 11”) of a GuTS71-08 strain seedling clone.

  The component may be a component contained in a water exudate obtained by immersing a stem portion of the licorice plant strain in water and leaving it at 15 to 35 ° C. for 10 minutes to 48 hours to exude.

  According to the present invention, it can be supplied stably and has an excellent growth promoting action on plants.

It is the figure which compared pure water and the water exudate which exuded from the licorice plant strain, after leaving still at room temperature for 10 days from preparation of a water exudate. (A)-(c) is a figure which shows the salad mizuna 14 days after sowing cultivated using pure water, GuIV2-1 water, and GuIV2-2 water. (A), (b) is a figure which shows the result of having measured the plant height and root length of the salad mizuna 14 days after sowing. It is a figure which shows the salad mizuna 27 days after exchange to a nutrient solution fertilizer. (A)-(c) is a figure which shows the sweet basil 14 days after sowing cultivated using pure water, GuIV2-1 water, and GuIV2-2 water. (A), (b) is a figure which shows the result of having measured the plant height and root length of sweet basil 14 days after sowing. It is a figure which shows the sweet basil 21 days after replacement | exchange to a nutrient solution fertilizer. (A), (b) is a figure which shows the cherry tomato 15 days after sowing cultivated using pure water and Gu # 11 water. It is a figure which shows the cherry tomato 29 days after sowing. It is a figure which shows the plant height distribution of cherry tomato 29 days after sowing. It is a figure which shows the average value of the plant height of cherry tomato 29 days after sowing. It is a figure which shows the corn 15 days after sowing cultivated using pure water and Gu # 11 water. (A), (b) is a figure which shows the root of the corn 17 days after sowing cultivated using pure water and Gu # 11 water. It is a figure which shows the corn 26 days after sowing cultivated using pure water and Gu # 11 water. It is a figure which shows the average value of the plant height of the above-ground part of the corn 15 days after seeding | cultivated using the pure water and Gu # 11 water. It is a figure which shows the result of having compared the germination rate of the ginseng in the test group of 15 degreeC. (A), (b) is a figure which shows the Panax ginseng 113 days after seeding | cultivation cultivated using the pure water and Gu water in the test plot of 15 degreeC. (A), (b) is a figure which shows the ginseng 116 days after sowing cultivated using the pure water and Gu water in the test plot of 15 degreeC. It is a figure which shows the result of having compared the ratio of the seedling in which the number of leaves of ginseng is 160 days after sowing. It is a figure which shows the result of having compared the germination rate of the ginseng in the test plot of 4 degreeC. (A), (b) is a figure which shows the ginseng after 106 days of seeding | cultivation cultivated using the pure water and Gu water in the test plot of 4 degreeC.

(Licorice plant strain)
The licorice plant strain containing the plant growth promoting component contained in the plant growth promoting agent according to the embodiment of the present invention is a licorice plant strain containing a large amount of glycyrrhizic acid useful as a medicinal component at the root, and is subcultured. A strain that can grow.

  In the present specification, the “Glycyrrhiza plant” is a plant containing glycyrrhizic acid in the root portion, but may be a plant containing glycyrrhizic acid in stems, leaves or the like other than the root portion. For example, G. uralensis, G. glabra, G. inflata, G. acanthocarpa, G. aspera, G. astragalina, G. bucharica, G. echinata, G. eglandulosa, G. foetida, G. foetidissima, G. gontscharovii, G. iconica, G. korshinskyi, G. lepidota, G. pallidiflora, G. squamulosa, G. triphylla, G. yunnanensis, varieties of these licorice plants are applicable Ural licorice and Spanish licorice are preferred, and Ural licorice is more preferred.

  The root part (underground part) broadly includes roots, strons, and the like, and the stalk part (stem and leaf part, above-ground part) widely includes stems, top buds, shoots, nodes, leaves, and the like. The stem section can be prepared using, for example, a known cutting means such as a scissors, a cutter, or a knife.

  Examples of the licorice plant strain include any of Uralanthus Gu2-2-1 strain, Gu2-3-2 strain, GuIV1 strain, GuIV2 strain, and Gu # 11 strain. The present applicant has made a self-deposit of these strains within the National Institute of Biomedical Research, Medicinal Plant Resource Research Center Tsukuba Research Department Breeding Physiology Laboratory (location: 1-2, Yahatadai, Tsukuba City, Ibaraki, Japan) Maintained and preserved. The Applicant guarantees that it will be distributed to a third party subject to compliance with each law if it falls under each item of Article 27-3 of the Japanese Patent Law Enforcement Regulations.

  Uralphanthus Gu2-2-1 strain, Gu2-3-2 strain, GuIV1 strain, GuIV2 strain, Gu # 11 strain are the nucleotide sequence of exon 1 to exon 3 of SQS (squalene synthase) 2 gene, the CYP88D6 gene It has a base sequence in the intron 7 part, a base sequence in the intron 1 to intron 2 part, and exon 3 to intron 4 part of the CYP72A154 gene. The base sequence in the exon 1 to exon 3 part of the SQS2 gene, the base sequence in the intron 7 part of the CYP88D6 gene, and the base sequence in the intron 1 to intron 2 and exon 3 to intron 4 part of the CYP72A154 gene include glycyrrhizic acid. It is a sequence necessary for plants that can contain a large amount of secondary metabolites (such as liquiritin, isoliquiritin, and glycicmarin) at the roots, and can be propagated by passage.

  The sequences of SEQ ID NOs: 1 and 2 in the sequence listing are partial sequences of base sequences in exon 1 to exon 3 of the SQS2 gene. The sequences of SEQ ID NOs: 3 to 6 in the sequence listing are partial sequences of the base sequence in the intron 7 portion of the CYP88D6 gene. The sequences of SEQ ID NOs: 7 to 9 in the Sequence Listing are base sequences in exon 1 to exon 5 part including intron 1 to intron 2 part and exon 3 to intron 4 part of CYP72A154 gene. The sequences of SEQ ID NOs: 1 to 9 include, in the gene of licorice plant strain, partial sequence of base sequence in exon 1 to exon 3 part of SQS2 gene, partial sequence of base sequence in intron 7 part of CYP88D6 gene, and / or CYP72A154 gene is a sequence useful for determining whether or not it has a base sequence in intron 1 to intron 2 and exon 3 to intron 4 of the gene, and the gene has any sequence of SEQ ID NOs: 1 to 9 The plant strain can be identified as a strain containing a plant growth promoting component having an excellent growth promoting action on the plant in the stem.

  This licorice plant strain has a partial sequence of base sequence in exon 1 to exon 3 of SQS2 gene, a partial sequence of base sequence in intron 7 of CYP88D6 gene, or intron 1 to intron 2 of exon 3 of CYP72A154 gene. It is a strain having a base sequence in the intron 4 portion, and has any one of these partial sequences of SEQ ID NOs: 1 to 9. The licorice plant strain may be a licorice plant strain having a part or all of the sequences of SEQ ID NOs: 1 to 9.

  This licorice plant strain has a base sequence in exon 1 to exon 3 part of SQS2 gene, a base sequence in intron 7 part of CYP88D6 gene, or a base sequence in intron 1 to intron 2 and exon 3 to intron 4 part of CYP72A154 gene May have a nucleotide sequence of 90% or more, preferably 95% or more, more preferably 99% or more.

  The licorice plant strain having any sequence of SEQ ID NOs: 1 to 9 is a strain containing a plant growth promoting component in the stem, and the plant growth promoting component is detected by detecting the sequence of SEQ ID NOs: 1 to 9. The containing strain can be identified. The method described in the specification of Japanese Patent Application No. 2013-049279 etc. can be utilized for the method for detecting the sequences of SEQ ID NOs: 1 to 9, the method for growing the licorice plant strain, and the cultivation method. Since this licorice plant strain is inexpensive and can be grown by a simple operation, the plant growth promoting component contained in the licorice plant strain can be stably supplied.

(Plant growth promoter)
The plant growth promoter according to the embodiment of the present invention contains a plant growth promoting component having an excellent growth promoting action on plants.

  The plant growth promoting component is obtained by excising the stem part (stems and leaves, above-ground part) of the plant of the genus genus plant having any one of SEQ ID NOs: 1 to 9 from the root of the strain, and further from the stem part. A stalk section of about 0.5 to 10 cm length or a shredded stalk section is prepared by cutting the leaves, and the stalk section is immediately immersed in 2 L of pure water, 15 to 35 ° C., 10 minutes to 48 hours, more preferably Is a component contained in a water exudate exuded from a prepared stem section after standing at 25 ° C. for 65 to 85 minutes.

  The plant growth promoter may be a water exudate exuded from the stems of a plurality of licorice plant strains having any one sequence of SEQ ID NOs: 1 to 9, and among the sequences of SEQ ID NOs: 1 to 9, It may be a water exudate exuded from the stem portion of a licorice plant strain having a partial or entire sequence.

  As the stem section, one containing an arbitrary number of nodes can be used. For example, it may be one node, three or more nodes, or may be shredded. Stem slices can be prepared, for example, using a known cutting means such as a scissors, a cutter, or a knife.

  The water in which the stem section is immersed is not limited to pure water, and an aqueous solution containing components necessary for the growth of plants such as nitrogen, phosphorus, potassium and metal components can be used, and tap water or well water can be used. Can also be used. Further, the temperature and time for immersing the stem section in pure water, and the amount of pure water to be immersed can be appropriately changed depending on the number, thickness and length of the stem section. Further, the immersion time can be shortened by chopping the stem section.

  The density | concentration and quantity of this plant growth promotion component can be suitably changed with the kind of plant to grow.

  Although this plant growth promoter is not particularly limited, it can be produced by foliar spray treatment, soil mixing treatment, soil irrigation treatment, seed treatment, hydroponics, addition treatment to tissue culture medium, etc. Applicable to. In addition, a general method of using the plant growth promoter is to promote plant growth in the soil activated by the plant growth promoter. However, the plant growth promoter is directly promoted without using the soil. It is also possible to activate the soil without aiming to promote plant growth. Moreover, soil activation and promotion of plant growth need not be performed at the same place, and the activated soil may be moved to an appropriate place.

  In addition, the plant growth promoter can be further mixed with one or more additives such as vitamins, sugars, amino acids, nucleic acids, organic acids, proteins, and alcohols.

  The plant to which the present plant growth promoter is applied is not particularly limited, but examples thereof include gramineous grains such as rice, wheat and corn (Example corn), eggplant, tomato, pepper, capsicum and the like. Fruits of solanaceae (Example cherry tomatoes), fruits and vegetables such as strawberries, mustard, cabbage, watercress, komatsuna, cruciferous leafy vegetables such as salad mizuna (example salad mizuna), perilla, basil, mint, oregano, thyme, Herbs such as lemon balm (example sweet basil), ginseng plants such as ginseng, tochiban ginseng, American carrot, sorghum, etc. (example ginseng), spinach, lettuce, leafy vegetables such as salad vegetables, radish, carrot, potato, taro, etc. Root vegetables, peaches, fruit trees such as grapes, flower buds such as pansies, lilies , Zoysia tenuifolia, grasses such as Bentoshiba and the like.

  The plant growth promoter can be used in combination with commercially available agricultural chemicals and fertilizers. For example, this plant growth promoter can contain at least one selected from nitrogen, phosphoric acid, potassium, calcium, magnesium, sulfur and the like as a fertilizer component. Further, as a fertilizer component, at least one selected from iron, copper, zinc, manganese, molybdic acid, chlorine, boron and the like can be further contained.

  By applying this plant growth promoter, the growth of the plant is promoted, the quality of the crop is improved, the resistance of the crop is improved, the resistance to the pest of the crop is improved, the fruit set of fruit trees is increased and the fruit is reduced, the sugar content of the fruit It is effective for improving the taste, improving the color and gloss of flowers, and improving the shelf life of crops.

  The plant growth promoter may be a water exudate exuded from the stem part of a licorice plant that does not have any sequence of SEQ ID NOs: 1 to 9. For example, hydroponic cultivation of Ural licorice, Spanish licorice, which does not have any sequence of SEQ ID NOS: 1-9, and water exudates exuded from stem sections obtained from the stems of grown larval licorice, Spanish licorice, It can also be used as a growth promoter.

  EXAMPLES Hereinafter, although an Example demonstrates this invention in detail, an Example does not limit this invention.

(Preparation of water exudate)
The stem of GuIV2 strain, which was hydroponically cultivated in a growth chamber with a temperature of 25 ° C., a light period of 16 hours, and a relative humidity of 60%, was excised from the root of the strain, and all leaves were excised from the stem. Stem slices containing were prepared. The prepared stem section was immediately immersed in 2 L of pure water and allowed to stand at 25 ° C. for 65 to 85 minutes to obtain a water exudate that exuded from the prepared stem section.

  FIG. 1 is a view comparing pure water and a water exudate exuded from a licorice plant strain after standing at room temperature for 10 days from the preparation of the water exudate. In FIG. 1, GuIV2-1 water is a water exudate when the volume of pure water is 2 L, the number of stem sections is 70, and the immersion time in pure water is 65 minutes. GuIV2-2 water is pure water. This is a water exudate when the volume of 2L is 47, the number of stem sections is 47, and the immersion time in pure water is 85 minutes. As shown in the figure, it was revealed that the color intensity of the water exudate after standing at room temperature for 10 days from the preparation was affected by the number of stem sections from the immersion time in pure water.

(Cultivation of salad mizuna)
Hydroponic culture kit for home use Aqua Planter Float Mini (manufactured by Aquaculture Co.) is filled with pure water, GuIV2-1 water, GuIV2-2 water, and 50 seeds of salad water rape seeds are seeded on the nursery sponge. Cultivated in a growth chamber (temperature 20 ° C., light period 14 hours, relative humidity 60%).

Fig.2 (a)-(c) is a figure which shows the salad mizuna 14 days after sowing cultivated using pure water, GuIV2-1 water, and GuIV2-2 water. Table 1 is a table comparing the germination number (cotyledon development number) and germination rate (cotyledon development rate) of salad mizuna 14 days after sowing. In FIG. 2 and Table 1, pure water is salad mizuna cultivated using pure water, GuIV2-1 water is salad mizuna cultivated using GuIV2-1 water of FIG. 1, and GuIV2-2. The water is salad mizuna grown using GuIV2-2 water in FIG. Hereinafter, in the figure, pure water in the table indicates a plant cultivated using pure water, and Gu water (GuIV2-1 water, GuIV2-2 water, etc.) is a water exudate exuded from a licorice plant strain ( A plant cultivated using (Gu water).

  2 (a) to (c), as shown in Table 1, the salad mizuna cultivated using GuIV2-1 water and GuIV2-2 water had better germination number and germination rate than pure water. . Moreover, GuIV2-1 water with many stem slices at the time of preparation had a higher germination number and germination rate than GuIV2-2 water.

  Further, thinning was performed while leaving 10 individuals with good growth in each test area, and the plant height and root length of the thinned plants were measured. FIGS. 3A and 3B are diagrams showing the results of measuring the plant height and root length of salad mizuna 14 days after sowing. As shown in the figure, compared to pure water, the salad mizuna cultivated using GuIV2-1 water and GuIV2-2 water grew better.

  Furthermore, after 24 days of sowing, the solution in each test group was replaced with a nutrient solution fertilizer HYPONEX (registered trademark) 6.5-6-19 (manufactured by Hyponex Japan) 1 g / L, and cultivation was continued. FIG. 4: is a figure which shows the salad mizuna 27 days after replacement | exchange for nutrient solution fertilizer. In FIG. 4, pure water, GuIV2-1 water, and GuIV2-2 water indicate salad edible plants cultivated using pure water, GuIV2-1 water, and GuIV2-2 water at the time of sowing. As shown in FIG. 4, the growth of the salad mizuna using GuIV2-1 water and GuIV2-2 water in the early stage of cultivation was better than the salad mizuna cultivated using pure water.

  From the above results, it was revealed that the water exudate exuded from the stem part of the plant body of the GuIV2 strain has a high germination promoting effect and an initial growth promoting effect.

(Cultivation of sweet basil)
Sweet basil was cultivated using pure water, GuIV2-1 water of FIG. 1, and GuIV2-2 water of FIG. After preparing pure water, GuIV2-1 water, and GuIV2-2 water and leaving it to stand for 14 days, as with the cultivation of salad mizuna, a hydroponic tank for household hydroponics kit Aqua Planter Float Mini (manufactured by Aquaculture) is used. , Pure water, GuIV2-1 water, GuIV2-2 water, 50 seeds of sweet basil seeds were sown on a seedbed sponge, the same conditions (growth chamber room, temperature 20 ° C., light period 14 hours, relative humidity 60 %).

Fig.5 (a)-(c) is a figure which shows the sweet basil 14 days after sowing cultivated using pure water, GuIV2-1 water, and GuIV2-2 water. Table 2 is a table comparing the germination number (cotyledon development number) and germination rate (cotyledon development rate) of sweet basil 14 days after sowing.

  As shown in FIGS. 5A to 5C and Table 2, sweet basil cultivated using GuIV2-1 water and GuIV2-2 water had better germination numbers and germination rates than pure water. .

  Further, thinning was performed while leaving 10 individuals with good growth in each test area, and the plant height and root length of the thinned plants were measured. 6 (a) and 6 (b) are diagrams showing the results of measuring the plant height and root length of sweet basil 14 days after sowing. As shown in the figure, sweet basil cultivated using GuIV2-1 water and GuIV2-2 water was better grown than pure water.

  Further, after 16 days of sowing, the solution in each test group was replaced with a nutrient solution fertilizer HYPONEX (registered trademark) 6.5-6-19 (manufactured by Hyponex Japan) 1 g / L, and cultivation was continued. Drawing 7 is a figure showing sweet basil 21 days after exchange to nutrient solution fertilizer. In FIG. 7, pure water, GuIV2-1 water, and GuIV2-2 water indicate sweet basil cultivated using pure water, GuIV2-1 water, and GuIV2-2 water at the time of sowing. As shown in the figure, sweet basil using GuIV2-1 water and GuIV2-2 water grew better than sweet basil grown using pure water at the initial stage of cultivation.

  From the above results, it was revealed that the water exudate exuded from the stem part of the plant body of the GuIV2 strain has a high germination promoting effect and an initial growth promoting effect.

(Cultivation of cherry tomatoes)
Pure water, Gu # 11 water, SOLANACEAE somato bean tomato genus (scientific name: Lycopersicon esculentum Mill. Var. Cerasiforme (Dunal) A.Gray, trade name: mini tomato "sugar lamp" Sakata seed (registered trademark) )) Was cultivated. Gu # 11 water was obtained by excising the stem part of the plant body of the Gu # 11 strain which was hydroponically cultivated in a growth chamber room at a temperature of 25 ° C., a light period of 16 hours and a relative humidity of 60% from the root of the strain. All the leaves were excised from the part to prepare 60 stalk slices containing 2 nodes. The prepared 60 stalk slices were immediately immersed in 2 L of pure water and allowed to stand at 25 ° C. for 60 minutes. It is a water exudate exuded from 60 sections.

  After sufficiently holding pure water and Gu # 11 water in each sponge of the aqua planter, 50 cherry tomato seeds were placed on each sponge, and cultivation was started with pure water and Gu # 11 water. In order to prevent drying, from the sowing to the 19th day, the aqua planter was covered with polyethylene wrap and cultivated under conditions of a temperature of 25 ° C., a light period of 16 hours, and a relative humidity of 60%.

  5 days after sowing, rooting of cherry tomato seeds was observed, and the number of rooting on the 8th day was 34 in the water treatment zone (cultivation with pure water) and cultivation in the Gu # 11 water treatment zone (Gu # 11 water). ), The number of germination (budding) individuals is 1 in the water treatment area, 9 in the Gu # 11 water treatment area, and germination is dominant in the cherry tomatoes in the Gu # 11 water treatment area. there were. FIGS. 8A and 8B are diagrams showing cherry tomatoes 15 days after sowing cultivated using pure water and Gu # 11 water. As shown in the figure, the number of the cotyledons fully developed after 15 days after sowing was 6 for the cherry tomatoes in the water-treated area and 9 for the cherry tomatoes in the Gu # 11 water-treated area. For this reason, the cherry tomatoes grown using Gu # 11 water grew better.

  15 days after sowing, the nutrient solution was changed to 1 g / L of nutrient solution fertilizer HYPONEX (registered trademark) 6.5-6-19 (manufactured by Hyponex Japan) in both the pure water and Gu # 11 water treatment areas. When the water level dropped, the nutrient solution was replenished to maintain the water level and continue cultivation. FIG. 9 is a diagram showing cherry tomatoes 29 days after sowing. In FIG. 9, pure water and Gu # 11 water indicate cherry tomatoes grown using pure water and Gu # 11 water at the time of sowing. As shown in the figure, the growth of cherry tomatoes cultivated using Gu # 11 water was better.

  29 days after sowing, the sponge of the cherry tomato support was divided in half, and growth studies were carried out on approximately half of the growth strains (water treatment group: 9 strains, Gu # 11 water treatment group: 18 strains). FIG. 10 is a graph showing the distribution of cherry tomato plant height 29 days after sowing. Moreover, FIG. 11 is a figure which shows the average value of the plant height of the cherry tomato 29 days after sowing. As shown in the figure, in the test plot (n = 18) where Gu # 11 water was applied during the initial cultivation period of cherry tomatoes, the average plant height was 38.7 mm (standard error 4.4 mm), and it was initially grown in pure water. In the test plot (n = 9), the average plant height was 23.8 mm (standard error 5.6 mm). For this reason, the cherry tomatoes grown using Gu # 11 water grew better.

  From the above results, the plant cultivated using the water exudate exuded from the stem of the plant body of the Gu # 11 strain has a high cotyledon development rate, and the subsequent plant growth is also good. It was revealed that the germination promoting effect and the initial growth promoting effect were high.

(Corn cultivation)
Cultivate corn of POACEAE Gramineae genus (scientific name: Zea mays L., trade name: corn "sweet corn, peter corn, honey bantam" Sakata Seed (registered trademark)) using pure water and Gu # 11 water did. Gu # 11 water was the same as the water exudate from which the cherry tomatoes were cultivated, and the water exudate exuded from the plant body of the Gu # 11 strain was used. Each sponge of the aqua planter was sufficiently retained with pure water and Gu # 11 water, and then 12 corn seeds were placed on each sponge, and cultivation was started with pure water and Gu # 11 water. To prevent dryness, from the sowing to the 10th day, the aqua planter is covered with polyethylene wrap (if the plant height is extended, a plastic cup), and the growth is performed at a temperature of 25 ° C., a light period of 16 hours, and a relative humidity of 60%. Cultivation was carried out in a chamber.

  Comparing the number of germination (emergence) 8 days after sowing, the growth of 8 strains in the water treatment zone (cultivation with pure water), of which 4 strains are good, the cultivation with Gu # 11 water treatment zone (Gu # 11 water cultivation) ) 6 shares, of which 6 were all growing well. Ten days after sowing, the number of strains with good growth became 3 in the water treatment zone, and the tips of the 4 strains began to turn black. In the Gu # 11 water treatment section, 4 strains grew well and the tips of the 2 strains turned black. On the same day, the wrap and plastic cup were removed to prevent drying.

  FIG. 12 is a diagram showing corn grown 15 days after sowing cultivated using pure water and Gu # 11 water. As shown in the figure, in the water treatment zone, the growth of 4 strains was good, 3 in which the tip was withered, 2 in the growth failure, and 4 in the Gu # 11 water treatment zone. There were 2 strains with good growth and withered tips.

  15 days after sowing, the nutrient solution was changed to 1 g / L of nutrient solution fertilizer HYPONEX (registered trademark) 6.5-6-19 (manufactured by Hyponex Japan) in both the pure water and Gu # 11 water treatment areas. When the water level dropped, the nutrient solution was replenished to maintain the water level and continue cultivation. FIGS. 13A and 13B are diagrams showing corn roots 17 days after sowing cultivated using pure water and Gu # 11 water. As shown in the figure, the roots of corn cultivated using Gu # 11 water grew better.

  FIG. 14 is a diagram showing corn cultivated with pure water and Gu # 11 water 26 days after sowing. As shown in the figure, corn cultivated using Gu # 11 water had a larger number of strains with good growth, and the above-ground part was also growing.

  In addition, on the 15th and 26th days after sowing, the plant heights of the above-ground parts of the 4 strains in both wards with good growth were measured. FIG. 15 is a diagram showing an average value of the plant height of the above-ground part of corn cultivated using pure water and Gu # 11 water. As shown in the figure, after 15 days of sowing, in the test section (n = 4) where Gu # 11 water was applied, the average plant height was 16.0 cm (standard error 0.9 cm), and the initial cultivation was performed with pure water. In the plot (n = 4), the average plant height was 7.2 cm (standard error 1.6 cm), and the corn in the Gu # 11 water treatment plot was found to be significantly higher (p <0.01). . In addition, 26 days after sowing, in the test plot (n = 4) to which Gu # 11 water was applied, the average plant height was 49.5 cm (standard error 3.2 cm), and the test plot was initially cultivated in pure water (n = 4). ) Showed that the average plant height was 39.0 cm (standard error 5.9 cm), and the average plant height was higher in the corn in the Gu # 11 water treatment area.

  From the above results, it has been clarified that the water exudate exuded from the stem part of the plant body of the Gu # 11 strain has a high effect of remarkably promoting the initial growth after germination.

(Cultivation of ginseng)
Panax ginseng CAMey. (Scientific name: Panax ginseng CAMey.) Was cultivated using pure water and Gu water. For Gu water, the stems of plant bodies of GuIV1, GuIV2, and Gu # 11 strains that were hydroponically cultivated in a growth chamber with a temperature of 25 ° C., a light period of 16 hours, and a relative humidity of 60% were excised from the root of the strain Furthermore, about 14 g (about 50 to 70) of stem sections including 2 nodes were prepared by cutting all leaves from the stem, and about 14 g of the prepared stem sections were immediately immersed in 2 L of pure water at 25 ° C. This is a water exudate that exudes from about 14 g of the prepared stem section after standing for 60 minutes.

  A tree pot (manufactured by Yamauri Seisakusho Co., Ltd.) was filled with vermiculite, seeded with 40 seeds of ginseng sprouted from Nagano Prefecture, and cultivated while supplying pure water and Gu water, respectively. Cultivation was carried out in 2 test sections of a 15 ° C. incubator and a 4 ° C. cool box.

  In the 15 ° C. test zone (cultivation at 15 ° C.), germination (budding) was observed 54 days after sowing, and the number of germinations after 160 days of sowing was 12 in the water treatment zone (cultivation with pure water), Gu water. There were 19 strains in the treatment zone (cultivation with Gu water). FIG. 16 is a diagram showing the results of comparing the germination rate of ginseng in the 15 ° C. test plot. Moreover, Fig.17 (a), (b) is a figure which shows the ginseng after 113 days of seeding | cultivation cultivated using the pure water and Gu water in the test plot of 15 degreeC. FIGS. 18 (a) and 18 (b) are views showing ginseng grown 116 days after sowing in pure water and Gu water in a 15 ° C. test zone. As shown in the figure, in cultivation at 15 ° C., Gu water had an effect of promoting the germination of ginseng.

  Moreover, the number of leaves of the ginseng seedling in the test section of 15 ° C. was compared in each treatment section. FIG. 19 is a diagram showing the results of comparing the proportion of seedlings with two leaves up to 160 days after sowing in the water treatment area and the Gu water treatment area. As shown in the figure, in the water treatment section, the proportion of seedlings with 2 leaves was 2.5% of the seeds sowed (8.3% of germinated seeds), whereas Gu water treatment In the ward, the proportion of seedlings with 2 leaves was 15% of the seeds sowed (31.6% of germinated seeds), indicating that Gu water has the effect of increasing the number of leaves. In addition, the ginseng usually has one leaf per year, but by cultivating it with Gu water, germination was promoted and the possibility of obtaining roots as medicinal sites earlier was shown.

  In the test group at 4 ° C. (cultivation at 4 ° C.), germination was observed 106 days after sowing, and the number of germination was 3 in the water treatment group and 9 in the Gu water treatment group. In addition, the number of germinations after 117 days after sowing was 8 in the water treatment zone and 14 in the Gu water treatment zone. FIG. 20 is a diagram showing the results of comparison of the germination rate of ginseng in the 4 ° C. test plot. Moreover, FIG. 21 (a), (b) is a figure which shows the ginseng after 106 days of seeding | cultivation cultivated using the pure water and Gu water in the 4 degreeC test plot. As shown in the figure, in cultivation at 4 ° C., Gu water had an effect of promoting the germination of ginseng.

  From the above results, it was revealed that the water exudate exuded from the stems of the plant bodies of the GuIV1 strain, the GuIV2 strain, and the Gu # 11 strain had an effect of promoting plant germination. Moreover, it became clear that cultivation at 15 ° C. has an effect of increasing the number of leaves of plants.

Claims (3)

A plant growth promoter comprising a water exudate of a stem portion of a licorice plant strain having a DNA represented by any one of SEQ ID NOs: 1 to 9 in the Sequence Listing. The sequences are the Uralanthus Gu2-2-1 strain, Gu2-3-2 strain, GuTS71-0.
8IV1 strain (GuIV1 strain), GuTS71-08IV2 strain (GuIV2 strain), or G
SQ in the strain identification number 11 strain (Gu # 11 strain) of the uTS71-08 strain seedling clone
The plant growth promoter according to claim 1, which is a partial sequence of S (squalene synthase) 2 gene, CYP88D6 gene or CYP72A154 gene. Immersing the stems of the previous SL Glycyrrhiza plant lines of water, 15 to 35 ° C., 10 minutes to 48 hours, allowed to stand to obtain exuded water exudate, it claim 1 or 2, characterized in A method for producing a plant growth promoter.