JP6100451B2 - Topical skin preparation - Google Patents
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Description
本発明はコラーゲンの産生を促進するコラーゲン産生促進剤、コラーゲン減少による老化等の現象の防止・緩和に有用な食品及び皮膚外用剤に関する。 The present invention relates to a collagen production promoter that promotes the production of collagen, a food useful for preventing or alleviating a phenomenon such as aging due to a decrease in collagen, and an external preparation for skin.
コラーゲンは、身体の結合組織を構成する主要タンパク質であり、皮膚、骨、軟骨、腱、血管壁などに大量に存在し、身体の全タンパク量の約１／３を占めている。コラーゲンは皮膚や骨の構成要素として構造の維持に役立ち、また細胞間で細胞の保護や接着などの生理的役割を果たしている。炎症や創傷で害された組織の治癒過程においても、真皮線維芽細胞におけるコラーゲン等の線維成分の産生が重要な役割を担うことが報告されている。 Collagen is a major protein that constitutes the connective tissue of the body, and is present in large amounts in skin, bone, cartilage, tendon, blood vessel wall, etc., and occupies about 1/3 of the total protein amount of the body. Collagen helps maintain structure as a component of skin and bone and plays a physiological role such as cell protection and adhesion between cells. It has been reported that the production of fiber components such as collagen in dermal fibroblasts plays an important role in the healing process of tissues damaged by inflammation and wounds.
コラーゲンは加齢による新陳代謝の衰えや、紫外線やストレスなどの外的要因によって減少する。皮膚ではその乾燥重量の70％をコラーゲンが占めており、コラーゲンの減少にともなって弾力性や柔軟性が失われ、ひいては、しわやたるみといった肌の加齢変化現象を生じさせる。また骨の組織がもろくなったり、炎症や創傷の際の回復が遅れるなど、様々な問題を生じさせる。 Collagen decreases due to deterioration of metabolism due to aging and external factors such as ultraviolet rays and stress. Collagen accounts for 70% of the dry weight of the skin, and as the collagen decreases, elasticity and flexibility are lost, and as a result, aging changes such as wrinkles and sagging occur. It also causes various problems such as fragile bone tissue and delayed recovery from inflammation and wounds.
高分子であるコラーゲンは消化管や皮膚から吸収されにくいので、コラーゲン自体を摂食したり皮膚に適用しても、身体組織のコラーゲン増進効果は少ないと言われている。従って、身体自身に本来備わっている自然のコラーゲン産生能を活性化し、コラーゲンの産生を促進することにより、コラーゲン不足による老化等の問題を予防するような食品や皮膚外用剤が求められてきた。 Since collagen, which is a polymer, is difficult to be absorbed from the digestive tract and skin, it is said that even if the collagen itself is eaten or applied to the skin, the collagen promoting effect on the body tissue is small. Accordingly, there has been a demand for foods and external preparations for skin that activate the natural collagen-producing ability inherent in the body itself and promote the production of collagen, thereby preventing problems such as aging due to collagen deficiency.
特開２０００−９３１２１号公報記載の発明は、クマザサ、アマチャヅル、エゾウコギ、ベタイン、大豆サポニンから選択される物質をコラーゲンペプチドと共に摂取することにより、プロリン等のコラーゲン由来のアミノ酸の消化吸収を増進し、もって生体内のコラーゲン合成を促進するコラーゲン合成促進剤用組成物である。この文献においては、コラーゲンペプチドの吸収を高める吸収促進成分の一つとしてエゾウコギが記載されている。 The invention described in JP 2000-93121 A promotes digestion and absorption of amino acids derived from collagen such as proline by ingesting together with a collagen peptide a substance selected from Kumazasa, Achacharu, Ezoukogi, Betaine, and Soybean Saponin, It is a composition for a collagen synthesis promoter that promotes in vivo collagen synthesis. In this document, Ezocogi is described as one of absorption promoting components that enhance the absorption of collagen peptides.
特開２００５−２５５５２７号公報記載の発明は、パフィア属に属する植物、キランソウ属に属する植物及びPhaponticum属に属する植物からなる群から選択される植物の抽出物と、他の動植物由来成分と、ビタミン等のその他の成分とを配合することにより生体内のコラーゲン合成を促進するコラーゲン合成促進剤用組成物である。この文献においては、主たる植物抽出物に補助的に配合される多数の動植物由来成分の一つとして、エゾウコギ抽出物が記載されている。
本発明においては、コラーゲン産生を促進し、コラーゲンの減少にともなう、はり・弾力の低下、ひいては、しわやたるみといった肌の加齢変化を予防・改善しうる新規のコラーゲン産生促進剤、及びそれを配合した新規の食品及び皮膚外用剤を提供することを目的とする。 In the present invention, a novel collagen production promoter capable of promoting collagen production and preventing or improving age-related changes in skin such as wrinkles and sagging as a result of collagen decrease, and An object is to provide a novel food and a topical skin preparation.
前記目的を達成するために、本発明者らは、安全性に優れた物質の中から、コラーゲン産生促進効果を発現させる物質を得るべく鋭意研究を重ねた結果、従来は強壮作用や抗ストレス作用を有する物質として知られ、他のコラーゲン産生促進剤に補助的成分として配合されていたエゾウコギ抽出物が、単独でも顕著なコラーゲンの産生促進効果を有することを見出し、本発明を完成するに至った。 In order to achieve the above object, the present inventors have conducted intensive research to obtain a substance that exhibits a collagen production promoting effect from substances having excellent safety. It has been found that an extract of Ekogigi, which is known as a substance having an active ingredient and blended as an auxiliary component with other collagen production promoters, has a remarkable collagen production promotion effect by itself, and has led to the completion of the present invention. .
エゾウコギ（Siberian ginseng、学名Acanthopanex Senticosusu Harms）はウコギ科の落葉低木であり、滋養・強壮作用や抗ストレス作用のある薬用植物として知られている。本発明に用いられるエゾウコギ抽出物は、エゾウコギの茎、根及び葉などから水又は有機溶媒で抽出する。抽出に用いる有機溶媒は極性溶媒であっても非極性溶媒であってもよく、抽出方法にも特に制限はない。 Siberian ginseng (scientific name Acanthopanex Senticosusu Harms) is a deciduous shrub of the family Araceae and is known as a medicinal plant with nourishing, tonic and anti-stress effects. The sorghum extract used in the present invention is extracted from stalks, roots, leaves and the like of sorghum with water or an organic solvent. The organic solvent used for extraction may be a polar solvent or a nonpolar solvent, and the extraction method is not particularly limited.
本発明のコラーゲン産生促進剤におけるエゾウコギ抽出物の配合量は剤型や用途に応じて調整することができるが、安定性、安全性などの点から希釈して用いることが好ましい。発明者の実験によれば、エゾウコギ抽出物を単独で用いても、皮膚線維芽細胞のコラーゲン産生機能を高め、良好なコラーゲン産生促進作用を発揮する。比較的低濃度のエゾウコギ抽出物でも効果があるため、これを食品及び皮膚外用剤に安全に配合することができる。 The blending amount of the sorghum extract in the collagen production promoter of the present invention can be adjusted according to the dosage form and application, but it is preferably diluted from the viewpoint of stability and safety. According to the inventor's experiment, even when the sorghum extract is used alone, it enhances the collagen production function of dermal fibroblasts and exhibits a good collagen production promoting action. Even a relatively low concentration of Ekogi extract is effective, so it can be safely blended into foods and skin preparations.
本発明のコラーゲン産生促進剤を配合する食品には、必須成分であるエゾウコギ抽出物に加えて、必要に応じ、本発明の効果を損なわない範囲内で甘味料、防腐剤、酸化防止剤、香料、生薬や栄養補助剤等を配合できる。また、本発明のコラーゲン産生促進剤を配合する食品の形態は、粉末、散剤、顆粒剤、錠剤、カプセル剤等、経口摂取可能な様々な形態をとりうるほか、他の飲食品に添加することもできる。 The food containing the collagen production promoter of the present invention includes, in addition to the essential component Ezoukogi extract, if necessary, sweeteners, preservatives, antioxidants, and fragrances as long as the effects of the present invention are not impaired. Herbal medicines and nutritional supplements can be added. In addition, the form of the food containing the collagen production promoter of the present invention may take various forms that can be taken orally, such as powder, powder, granule, tablet, capsule, etc., and should be added to other foods and drinks. You can also.
本発明のコラーゲン産生促進剤を配合する皮膚外用剤には、必須成分であるエゾウコギ抽出物に加えて、必要に応じ、本発明の効果を損なわない範囲内で化粧料、医薬部外品、医薬品等に一般に用いられる各種成分、水性成分、保湿剤、増粘剤、紫外線吸収剤、紫外線散乱剤、防腐剤、酸化防止剤、香料、色剤、薬剤、生薬等を配合できる。また、本発明のコラーゲン産生促進剤を配合する皮膚外用剤の剤型は任意であり、例えば化粧水等の可溶化系、乳液、クリーム等の乳化系、あるいは軟膏、粉末分散系、水−油二層系、水−油−粉三層系等のような剤型でもかまわない。 The external preparation for skin that contains the collagen production promoter of the present invention includes cosmetics, quasi-drugs, and pharmaceuticals as long as they do not impair the effects of the present invention, if necessary, in addition to the essential component sorghum extract. For example, various components generally used in the field, aqueous components, humectants, thickeners, ultraviolet absorbers, ultraviolet scattering agents, preservatives, antioxidants, fragrances, colorants, drugs, crude drugs and the like can be blended. Further, the dosage form of the external preparation for skin containing the collagen production promoter of the present invention is arbitrary, for example, a solubilizing system such as lotion, an emulsifying system such as a milky lotion or cream, or an ointment, a powder dispersion system, or a water-oil. A dosage form such as a two-layer system or a water-oil-powder three-layer system may be used.
以下、本発明を試験的な実施例により詳細に説明する。この説明は本発明の実施方法を明らかにするためのものであり、例示した手法、数値等は発明を限定するものではない。 Hereinafter, the present invention will be described in detail with reference to experimental examples. This description is intended to clarify the method of carrying out the present invention, and the illustrated methods, numerical values, and the like do not limit the invention.
コラーゲン産生促進作用の検定試験
＜試料＞
試料は日本粉末株式会社製のエゾウコギエキスを用いた。同エキスは、中国産エゾウコギの根茎より水を抽出溶媒として抽出し、原生薬換算比が約20:1となるように調製されたものである。
＜試験方法＞
（試験対象）
コラーゲン産生能を有するヒト皮膚由来正常線維芽細胞株（TIG-120細胞）を財団法人ヒューマンサイエンス振興財団ヒューマンサイエンス研究資源バンクより購入し、コラーゲン産生能の評価対象として用いた。
Test for promoting collagen production <Sample>
As a sample, Ezokogi extract manufactured by Nippon Powder Co., Ltd. was used. The extract was extracted from Chinese rhododendron rhizomes using water as an extraction solvent, and the crude drug equivalent ratio was about 20: 1.
<Test method>
(Examination subject)
A human skin-derived normal fibroblast cell line (TIG-120 cell) having the ability to produce collagen was purchased from the Human Science Research Resource Bank, Japan Foundation for Human Sciences, and used as an evaluation target for collagen producing ability.
（試験方法）
TIG-120細胞株を基本培養液（ダルベッコ改変イーグル培地、培養補助因子としてウシ胎児血清を最終濃度10％で添加）を用いて12穴プラスチックプレート中に1穴あたり1.5×104個（培養面積の約10％を占める培養密度）播種し、5％CO2インキュベータ内で37℃で培養した。30％エタノールにて段階希釈した各濃度のエゾウコギエキスを基本培養液に1/100容量添加した培養液を調製し、播種後１日目にこれらの培養液へ交換（エゾウコギの最終希釈率10000〜1000倍）し、３日目に再度同希釈倍率の培養液に交換して更に１週間培養した。
(Test method)
TIG-120 cell line using a basic culture solution (Dulbecco's modified Eagle medium, fetal bovine serum added as a culture cofactor at a final concentration of 10%) in a 12-well plastic plate, 1.5 x 10 4 cells per well (culture area) Culture density occupying about 10%) and seeding at 37 ° C. in a 5% CO 2 incubator. Prepare a culture solution in which 1/10 volume of Ezoukogi extract of each concentration serially diluted with 30% ethanol is added to the basic culture solution, and replace it with these culture media on the 1st day after seeding (final dilution rate of Ezocogi is 10,000- 1000 times), and the medium was changed to the same dilution medium again on the third day, and further cultured for one week.
１週間の培養の後、各培養上清中のコラーゲン量を市販のコラーゲン定量用キット（ACBio社製ヒトコラーゲン タイプ１ ELISA）を用いて酵素免疫測定法（ELISA法）により定量した。対照群としては、30％エタノールを1/100容量（最終濃度0.3％）添加した培養液にて培養を行った細胞を用いた。コラーゲン量は各群３サンプルの平均値をとり、対照群を100％とした相対値で算出した。
（試験結果）
エゾウコギエキス処理群において対照群の細胞よりも培養液中のコラーゲン量の増加（最大で約2倍）が見られた。よって本発明品にはヒト皮膚の線維芽細胞のコラーゲン産生能を増加させる作用があることが明らかとなった。また、低濃度の希釈エキスを配合した培養液（最終希釈率10000倍）においても顕著な作用があることが明らかとなった。
(Test results)
The amount of collagen in the culture broth was increased (up to about 2 times) in the Ezocogi extract-treated group than in the control group. Therefore, it was revealed that the product of the present invention has an action of increasing the collagen production ability of human skin fibroblasts. In addition, it was revealed that there was a remarkable effect even in a culture solution (final dilution rate 10,000 times) containing a low concentration diluted extract.
食品製造例（1）美容食品の製造
常法により抽出したエゾウコギエキスを用いて、表２記載の組成を有する美容食品を製造した。
食品製造例（2）エゾウコギ飴の製造
実施例1と同様にして得られたエゾウコギエキスを乾燥して粉末化した後、常法を用いて、表3記載の組成を有するエゾウコギ飴を製造した。
皮膚外用剤製造例（1）美容乳液の製造
常法により抽出したエゾウコギエキスを用いて、表4記載の組成を有する美容クリームを製造した。
皮膚外用剤製造例（2）美容液の製造
常法により抽出したエゾウコギエキスを用いて、表５記載の組成を有する美容液を製造した。
本発明のコラーゲン産生促進剤は、ヒト皮膚の線維芽細胞におけるコラーゲン産生能を増加させる効果があり、またエゾウコギエキスは食品、皮膚外用剤などとして投与しうることが知られているから、コラーゲンの減少に伴う皮膚のはり・弾力の低下、ひいては、しわやたるみといった加齢変化を予防・改善し、加えて炎症や創傷の治癒過程にも有効な効果を期待できる。従って食品・化粧品等の製造上有用である。 The collagen production promoter of the present invention has an effect of increasing collagen production ability in human skin fibroblasts, and it is known that Ezocogi extract can be administered as a food, a topical skin preparation, etc. It can be expected to prevent and improve age-related changes such as the reduction of skin elasticity and elasticity, and wrinkles and sagging, as well as effective effects on inflammation and wound healing processes. Therefore, it is useful in the production of foods and cosmetics.
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| JP3900967B2 (en) * | 2002-02-22 | 2007-04-04 | 丸善製薬株式会社 | Antioxidants, skin cosmetics and beauty foods and drinks |
| JP2005139141A (en) * | 2003-11-10 | 2005-06-02 | Nippon Menaade Keshohin Kk | Collagen synthesis promoter and external preparation for the skin |
| FR2871379B1 (en) * | 2004-06-14 | 2006-09-29 | Ifremer | USE OF HIGHLY SULFATED POLYSACCHARIDE DERIVATIVES WITH LOW MOLECULE MASS TO MODULATE ANGIOGENESIS |
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| JP2007204449A (en) * | 2006-02-03 | 2007-08-16 | Pias Arise Kk | Matrix metalloprotease-9 and endothelin-1 production inhibitor, as well as a prophylactic / ameliorating agent for post-inflammation pigmentation containing the production inhibitor, and cosmetics |
| JP2008069097A (en) * | 2006-09-13 | 2008-03-27 | Aputo Kk | Mmp inhibitor containing transition metal fine particle |
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