JP6399520B2 - Optical measuring device and optical measuring method - Google Patents

Description

  The present invention relates to a light measurement device and a light measurement method, and in particular, a first measurement unit that measures the light intensity of first measurement light emitted from a first light source and transmitted through a measurement object, and light emitted from a second light source. The present invention relates to a light measurement device including a second measurement unit that measures second measurement light that is fluorescence from a measurement object irradiated with a light source.

  The inventors previously proposed a light-induced fluorescence measuring apparatus that can meet the demand for point-of-care testing (POCT) in the life science field. This measuring apparatus is a small and portable device that has a short examination time and high-precision evaluation analysis in the field where analysis is required. As a measuring method, for example, laser induced fluorescence (Laser Induced Fluorescence: LIF) is used.

  LIF is caused by exciting a measurement target (atom or molecule) by irradiating a laser beam that matches the excitation level (tuned wavelength) using the resonance transition of the target atom or molecule. This is a technique for measuring emitted luminescence (fluorescence). The concentration of the measurement object is calculated from the intensity of the fluorescence, and the temperature of the measurement object is calculated from the spectrum distribution of the fluorescence.

  Furthermore, in the light-induced fluorescence measurement device, the inventors replaced the colored glass filter that absorbs light other than the fluorescence emitted from the sample with an organic optical functional material (resin or the like) having optical performance equivalent to that of the colored glass filter. It was proposed to use an optical material in which an organic optical functional material is dispersed in a polymer substance.

  Conventionally, in the field of optoelectronics, organic optical functional materials have been used in, for example, laser media, optical filters, optical devices, and the like. Patent Document 1 proposes a solid dye laser medium in which polydimethylsiloxane (hereinafter also referred to as PDMS) contains, for example, a pyromethene dye. In Patent Document 2, in a microfluidic device in which an excitation light source and a detector are provided on a microchip having a flow path (microchannel) through which a sample is injected and discharged, an optical that selectively transmits only a necessary wavelength. For the filter region, an organic optical functional material in which a pigment belonging to, for example, the Sudan dye family is dispersed in PDMS is used.

  FIG. 10 shows a configuration example of the light-induced fluorescence measurement apparatus 301 proposed by the inventors. Note that the photo-induced fluorescence measurement device 301 shown in FIG. 10 is not known at the time of filing this application. The light-induced fluorescence measurement apparatus 301 includes a solid-state light source 303 such as a laser light source, a sample holding unit 305 that holds a sample to be measured, a fluorescence collection optical system including a lens and an optical filter, and a fluorescence measurement device 309 such as a photomultiplier tube. Including.

  Specifically, at least the light emitting surface 307 of the laser light source (solid light source 303) and the light receiving surface 311 of the photomultiplier tube (fluorescence measuring device 309) emit excitation light from the solid light source 303 and fluorescence emitted from the sample. It is embedded in or is in contact with a resin such as PDMS that is transparent to the light it contains. Further, the fluorescence collecting optical system formed in the optical path from the light emitting surface 307 to the light receiving surface 311 is also embedded in a transparent resin such as PDMS, and the transparent resin itself constitutes a part of the fluorescence collecting optical system. To do. That is, the light emitting surface 307 of the laser light source, the sample holder 305, the fluorescence collecting optical system, and the light receiving surface 311 of the photomultiplier tube are integrally held and positioned by a transparent resin.

  The fluorescence collecting optical system includes a first silicone resin 365, a first air chamber 367, a second silicone resin 369, a notch filter 371, a third silicone resin 373, a second air chamber 375, and an optical filter 351 (structure). 1). The optical filter 351 removes light other than the fluorescence to be measured from the self-fluorescent stray light and Raman light, and includes a dye diffusion suppression member 377 and a dye-containing silicone resin 379.

  The transparent resin structure that holds each component in an integrated manner is that the excitation light, autofluorescence generated when the sample holder is irradiated with the excitation light, and the excitation light from the resin as it travels through the resin. It is surrounded by a resin containing a pigment having a wavelength characteristic that absorbs the generated Raman light substantially uniformly. That is, the resin containing the pigment constitutes the housing 213 that holds the sample holder 205, the fluorescence measuring instrument 209, the fluorescence collection optical system, and the like.

  In the resin containing the pigment, at least a part of the solid light source 203 and the fluorescence measuring device 209 is embedded as necessary, and power is supplied to the solid light source 203 and the fluorescence measuring device 209 (not shown). Power source to be buried. Therefore, the position of the optical element or the like is unlikely to change and is stable against an external impact, and a holder for holding the optical element is not required. Therefore, the light-induced fluorescence measuring apparatus 201 is small and portable.

  In addition, the silicone resin that surrounds the light guiding space through which light passes is generated from the excitation light, autofluorescence generated when the sample holder is irradiated with excitation light, and from the resin when the excitation light travels through the resin. Because it contains a pigment with wavelength characteristics that absorbs Raman light almost uniformly, excitation light and fluorescence that is measurement light are not emitted to the outside of the device, and light from the outside remains inside the fluorescence collection optical system, etc. Never enter. Therefore, highly accurate measurement is possible. Further, stray light such as excitation light and its reflected / scattered light is once absorbed into the pigment-containing resin, and the complex multiple reflection of stray light hardly occurs. Therefore, the fluorescence collecting optical system does not need to cope with multiple reflections of complicated stray light, and is simplified. As a result, the light-induced fluorescence measuring apparatus 201 is miniaturized.

  As a measurement and analysis method of a sample using light, there is an absorbance measurement method in addition to the light-induced fluorescence measurement. The light absorbance measurement method irradiates a sample solution with light, and measures the light absorption ratio (that is, absorbance) by the substance to be measured in the sample solution that is generated when the irradiated light passes through the sample. This is a method for quantitatively analyzing the concentration of.

  Usually, in the absorbance measurement, light having a specific wavelength is irradiated, the light intensity after passing through the sample is measured with a measuring instrument (for example, an RGB sensor), and the absorbance is measured. In addition, the light to irradiate is not restricted to the light of a specific wavelength, You may make it irradiate white light.

  In recent years, for example, in the life science field, there is an increasing demand for simultaneously performing light-induced fluorescence measurement and absorbance measurement on the same sample. By measuring at the same time, it is easy to measure changes with time, and measurement with a small amount of sample is possible. In particular, providing a measuring instrument (hereinafter referred to as a composite sensor) capable of performing both measurements on a small amount of sample collected in a PCR tube meets the demand for point-of-care testing (POCT) in the life science field. Is important.

International Publication No. 2012/133920 Special table 2009-516162

  However, in order to provide a plurality of sensors in one light measuring device, there is room for designing a new sample holder, a light guide, and the like. In particular, with respect to the sample holder that enables the incidence and emission of light from a plurality of light sources, there has been a problem that the light guide window should be minimized in order to ensure light shielding properties to eliminate noise.

  Therefore, an object of the present invention is to provide a light measurement apparatus and the like that can simultaneously perform light-induced fluorescence measurement and absorbance measurement while the sample holder has a sufficient light shielding property.

  According to a first aspect of the present invention, there is provided a first light source, a first measurement unit that measures the light intensity of the first measurement light emitted from the first light source and transmitted through the measurement object, a second light source, and the second light source. A light measurement device including a second measurement unit that measures second measurement light that is fluorescence from a measurement object irradiated with light emitted from a light source, wherein the light emitted from the first light source is the first light. A light guide unit through which light passes until reaching one measurement unit and the light emitted from the second light source reaches the second measurement unit, a sample holding unit for holding the measurement object, and the light guide unit And a light-absorbing unit that surrounds the sample holding unit, wherein the light guide unit includes a first incident light emitted from the first light source, a first measurement light, and a second incident emitted from the second light source. The first resin transparent to the light and the second measurement light, and the sample holder is An incident light sharing light guide window for guiding incident light and the second incident light to the measurement object, and a first measurement light guide window for guiding the first measurement light from the measurement object to the first measurement unit. And a second measurement light guide window that guides the second measurement light from the measurement object to the second measurement unit.

  A second aspect of the present invention is the light measurement apparatus according to the first aspect, wherein a light receiving surface of the first measurement unit is inclined without being orthogonal to the optical axis of the first measurement light. It is characterized by being.

  A third aspect of the present invention is the light measurement apparatus according to the second aspect, wherein the light receiving surface is a plane including the optical axis of the second incident light and the optical axis of the second measurement light. It is characterized by tilting without being orthogonal.

  According to a fourth aspect of the present invention, there is provided the optical measurement apparatus according to any one of the first to third aspects, wherein the second light source of the light guide unit and the sample holding unit are disposed between The optical system further includes a first optical member that makes the optical axis of the two incident lights parallel to the optical axis of the first incident light.

  According to a fifth aspect of the present invention, there is provided the optical measurement apparatus according to the fourth aspect, wherein the optical measurement apparatus is configured to shield light having a wavelength different from that of the second measurement light between the sample holding unit and the second measurement unit. The optical system further includes two optical members, wherein the first optical member and the second optical member have optical characteristics that allow the first incident light to pass therethrough and reflect the second incident light.

  A sixth aspect of the present invention is the light measurement apparatus according to any one of the first to fifth aspects, wherein a dye is provided between the sample holding part and the second measurement part in the light guide part. The optical filter part which consists of 2nd resin which has these is further provided.

  A seventh aspect of the present invention is the light measurement device according to the sixth aspect, further comprising a dye diffusion suppression unit around the optical filter unit.

  An eighth aspect of the present invention is the light measurement apparatus according to the seventh aspect, wherein light having a wavelength different from that of the second measurement light is absorbed between the optical filter unit and the dye diffusion suppression unit. A second light absorber is further provided.

  A ninth aspect of the present invention is the light measurement apparatus according to the eighth aspect, wherein the second light absorption part is made of the same resin as the second resin constituting the optical filter part, It contains a second dye different from the dye.

  According to a tenth aspect of the present invention, there is provided a first light source, a first measurement unit that measures the light intensity of the first measurement light emitted from the first light source and transmitted through the measurement object, a second light source, and the second light source. A light measurement method comprising: a second measurement unit that measures second measurement light that is fluorescence from a measurement object irradiated with light emitted from a light source, wherein the light measurement device includes: A light guide unit through which light passes until light emitted from one light source reaches the first measurement unit and light emitted from the second light source reaches the second measurement unit, and the measurement object is held A sample holding part, and a light-absorbing part surrounding the light guide part and the sample holding part, wherein the light guide part includes first incident light emitted from the first light source, first measurement light, The first resin transparent to the second incident light and the second measurement light emitted from the second light source. The sample holding unit guides the first incident light and the second incident light to the measurement target; and the incident light shared light guide window; and the first measurement unit to the first measurement unit. A first measurement light guide window that guides one measurement light; a second measurement light guide window that guides the second measurement light from the measurement object to the second measurement unit; and the first light source and The light measurement method includes an irradiation step in which the second light source irradiates the measurement object with the first incident light and the second incident light through the incident light shared light guiding window, respectively.

  According to each aspect of the present invention, the first incident light and the second incident light can be guided to the measurement target from the same light guide window. Therefore, the light guide window of the sample holder can be minimized. As a result, it is possible to provide a light measuring device or the like that has a sample holder having sufficient light shielding properties and can simultaneously perform light-induced fluorescence measurement and absorbance measurement.

  Here, if the reflected light from the light receiving surface of the first measurement unit is incident on the sample holding unit again, the accuracy of the light-induced fluorescence measurement and the absorbance measurement is lowered.

  Therefore, according to the second aspect of the present invention, a part of the absorption measurement light or LIF excitation light (hereinafter referred to as absorption measurement surface incident light) incident on the light receiving surface of the absorption sensor is absorbed from the light source for absorption measurement. The light is reflected in a direction different from the optical axis connecting the sensors, and is incident on and absorbed by the light absorption unit surrounding the light guide unit. Therefore, highly accurate light measurement is facilitated.

  Furthermore, according to the 3rd viewpoint of this invention, it becomes possible to reduce more reliably that a stray light advances to a fluorescence collection optical system.

  Furthermore, according to the fourth aspect of the present invention, even when a light source including multi-wavelength light such as an LED is used instead of a laser light source as the LIF light source, only excitation light having a specific wavelength is guided to the sample. Easy to do.

  Further, according to the fourth aspect of the present invention, even if the LIF light source and the light absorption light source are not disposed adjacent to each other, the LIF incident light and the light absorption incident light are placed on the same light guide window of the sample holding unit. Can be guided. Therefore, the degree of freedom in designing the optical measurement device is improved, and it becomes easy to provide a small optical measurement device.

  Furthermore, according to the fifth aspect of the present invention, the first optical member and the second optical member can be provided as one type of optical member. Therefore, it becomes easy to reduce the incidence of stray light such as second incident light (excitation light) to the LIF sensor.

  Furthermore, according to the sixth aspect of the present invention, a colored glass filter that transmits only the second measurement light and absorbs the other light with a conventional light measurement device is not required, and is resistant to impact caused by carrying or carrying. It becomes possible to provide a light measurement device or the like.

  Furthermore, according to the seventh aspect of the present invention, the light diffusion device can suppress the diffusion of the dye in the optical filter unit to the outside and maintain the original filter function of the optical filter unit. Etc. can be provided.

  In view of the fact that it is preferable to suppress reflection and scattering of light in the light measurement device, there is an inhibiting factor in providing the dye diffusion suppression portion which is a different material. The seventh aspect of the present invention is characterized by the technical idea that the dye diffusion suppression unit is provided based on the recognition that the diffusion of the dye from the optical filter part having the dye into the housing may be a problem.

  Furthermore, according to the 8th viewpoint of this invention, it becomes possible to suppress reflection and scattering in an optical filter part, ensuring the light guide part which 2nd measurement light permeate | transmits.

  Furthermore, according to the ninth aspect of the present invention, since the second light absorption part and the optical filter part are made of the same material, both have the same refractive index and suppress the occurrence of reflection / scattering at the boundary surface. It becomes even easier.

It is a schematic diagram of the virtual structure of a composite sensor. 1 is an overall view of a composite sensor according to Embodiment 1 of the present invention. It is an enlarged view of the measurement light irradiation and light absorption measurement unit part which concerns on Example 1 of this invention. It is a schematic diagram of the structure of the composite sensor of this invention. FIG. 3 is an enlarged view of the periphery of the light absorption sensor according to Example 1. It is a general view of the composite sensor which concerns on Example 2 of this invention. It is a figure which shows a part of AA sectional drawing shown by FIG. It is an enlarged view of an optical filter part provided with the pigment | dye diffusion suppression member of this invention. It is an enlarged view of an optical filter part provided with the 2nd light absorption part of this invention. 1 is an overall view of a conventional light-induced fluorescence measuring apparatus previously proposed by the inventors of the present invention. FIG.

  Embodiments of the present invention will be described below with reference to the drawings.

  The embodiment of the present invention is not limited to the following examples.

  First, with reference to FIG. 1, the structure of a composite sensor that simply includes a light absorption sensor and a fluorescence sensor will be described. FIG. 1 is a schematic diagram of a virtual structure of a composite sensor.

  Referring to FIG. 1, in a transparent silicone resin 227, a sample holder 205 made of a PCR tube for holding a measurement sample, a light emitting surface 218 of a white light source 217 for absorbance measurement, a light source for light-induced fluorescence measurement (LIF) 219, a light emitting surface 220 of the light absorption sensor 221 for measuring the absorbance, a fluorescence collecting optical system 225 for collecting the fluorescence emitted from the sample and guiding it to the LIF sensor 223 for measuring the fluorescence, and the LIF The light receiving surface 224 of the sensor 223 is embedded. Furthermore, a silicone resin 229 containing a black pigment for absorbing stray light is provided so as to surround them.

  Further, the sample holding unit 205 includes an excitation light incident window 231 into which excitation light from the LIF light source 219 is incident, a fluorescence emission window 233 from which fluorescence is emitted, and an absorption incident window into which incident light from the white light source 217 for absorption measurement is incident. 235, an absorption exit window 237 through which the transmitted light from the white light source 217 for absorption measurement exits, and an excitation light exit window 243 through which the excitation light from the LIF light source 219 transmits through the sample holder 205 made of a PCR tube. Have

  According to the composite sensor configured as shown in FIG. 1, since it is surrounded by the silicone resin 229 containing the black pigment for absorbing stray light, stray light is not emitted to the outside. Further, by making the material of the transparent silicone resin 227 forming the light guide portion and the silicone resin 229 containing the black pigment the same, reflection at the interface between the two hardly occurs. Therefore, once stray light reaches the interface, it hardly enters the light guide part (transparent silicone) due to reflection at the interface, and noise is reduced.

  For example, of the LIF measurement light (excitation light) emitted from the LIF light source 219, the excitation light that has passed through the sample holding unit 205 without contributing to the excitation of the sample is incident on the black pigment-containing silicone resin 229, Absorbed by black pigment. Further, the absorbance measurement light from the absorbance measurement white light source 217 reaches the absorbance sensor 221 as indicated by an arrow.

  However, in the case of the structure of FIG. 1, the following problems occur. If only the LIF measuring device or the absorbance measuring device is not a composite sensor, a window for guiding incident light from the light source to the sample (hereinafter referred to as an incident light guiding window), and a sensor from the sample. The sample holder 205 is surrounded by a black pigment silicone resin except for a window for guiding the measurement light (hereinafter referred to as a measurement light guide window), thereby scattering by the sample holder 205. Noise can be reduced. However, in the composite sensor having the structure shown in FIG. 1, the LIF incident light guide window 231, the LIF measurement light guide window 233, the absorption incident light guide window 235, and the absorbance measurement light guide window 237 are guided in four places. A light window is required. Therefore, the light shielding property due to the black pigment-containing silicone resin surrounding the sample holding unit 205 becomes insufficient. For example, the scattered light of the measurement light for absorption by the sample holding unit 205 enters the fluorescence collecting optical system 225 for fluorescence measurement as noise. It may progress.

  Therefore, an embodiment of the composite sensor according to the present invention that can reduce noise will be described below.

  2 and 3 show a configuration example of the first embodiment. In this structure, the LIF light source (DPSS laser head) 303 and the sample holding unit 305 in the light-induced fluorescence measurement apparatus shown in FIG. 10 are replaced with an absorption measurement light source 1, a LIF light source 3, a sample holding unit 5, an absorption sensor 7, and the like. The measurement light irradiation / absorption measurement unit 9 including

Measurement light irradiation / absorption measurement unit
(A) Light source for absorption measurement As the light source 1 for absorption measurement, for example, an LED that emits white light is used.

(B) Light source for LIF
As the LIF light source 3, for example, an LED that emits light having a central wavelength of 532 nm, or a green that emits a laser beam having a wavelength of 532 nm, which is the second harmonic of a semiconductor-excited Nd: YVO ₄ laser (wavelength 1064 nm). A laser device is used.

(C) Sample (object to be measured)
In this example, Q-body, which is a fluorescent reagent that emits fluorescence having a wavelength of 570 nm to 580 nm when irradiated with a laser beam of 532 nm, was used as a measurement sample.

(D) Sample holder As the sample holder 5, for example, a polystyrene PCR tube is used. This PCR tube has a tapered tip, and even when a liquid sample is introduced, bubbles are hardly formed on the tip side of the PCR tube. The sample holder 5 for holding the sample is positioned so that light from the light source is irradiated on the tip side of the PCR tube constituting the sample holder.

  As shown in FIG. 4, which is a schematic diagram of the structure of the composite sensor of the present invention, the periphery of the sample holder 5 shown in FIGS. 2 and 3 (sample holder 205 in FIG. 4) Except for the optical window 239, the absorption measurement light guide window 237, and the LIF measurement light guide window 233, it is surrounded by the pigment-containing silicone resin 229.

(E) Absorption sensor
For example, an RGB color sensor is used as the light absorption sensor 7.

(F) Optical system (light guide, stray light countermeasure, etc.)
As shown in FIG. 3, the absorption measurement incident light 11 emitted from the absorption measurement light source 1 is an example of a first silicone resin 12 (a “first resin” in the claims of the present application) that is transparent to the light. For example, PDMS is used), and the light enters the sample holding unit 5 where the sample is held.

  The light guide A is provided between the light source 1 for absorbance measurement and the sample holder 5. A first 45-degree reflection-type notch filter 15 is provided at the end of the light guide A on the sample holder 5 side. A light guide C made of a transparent first silicone resin 12 is provided between the first 45-degree reflective notch filter 15 and the sample holder 5.

  The first 45-degree reflection type notch filter 15 transmits the incident light 11 for absorption measurement from the light source 1 for absorption measurement, and the incident angle of the light of wavelength 532 nm from the light source 3 for LIF and the light in the wavelength region in the vicinity thereof. When incident at 45 degrees, this light is reflected.

  Therefore, the incident light 11 for absorption measurement emitted from the light source 1 for absorption measurement passes through the light guide A, the first 45-degree reflection type notch filter 15 and the light guide C, and the sample in the sample holder 5. Is incident on.

  On the other hand, the LIF excitation light 19 emitted from the LIF light source 3 travels through the light guide B made of the first silicone resin 12 transparent to the light, and is reflected by the first 45-degree reflective notch filter 15. Then, it enters the sample of the sample holder 5 via the light guide C.

  The light guide B is provided between the LIF light source 3 and the first 45-degree reflective notch filter 15 and is connected so as to intersect the light guide C. The first 45 degree reflective notch filter 15 is transparent at the position corresponding to the light guide B intersecting the first 45 degree reflective notch filter 15 and on the surface opposite to the light incident surface of the first 45 degree reflective notch filter 15. A light guide B ′ made of the first silicone resin 12 is provided.

  Between the sample holder 5 and the light absorption sensor 7, a light guide D made of a transparent first silicone resin 12 is provided. The incident light 11 for absorption measurement incident on the sample of the sample holder 5 absorbs a part of light in a specific wavelength region based on the characteristics of the sample, and absorbs light as absorption measurement light 27 via the light guide D. Incident on the sensor 7. The measurement result by the light absorption sensor 7 is sent to a calculation unit (not shown), and the calculation unit determines the absorbance of the sample.

  On the other hand, the LIF excitation light 19 incident on the sample of the sample holder 5 via the light guide C excites the sample in the sample holder 5. The excited sample emits fluorescence 29 in a specific wavelength range based on the characteristics of the sample. This fluorescence is incident on the first dye-containing silicone resin 33 from the measurement light irradiation / absorption measurement unit 9 via the light guide E made of the transparent first silicone resin 12.

  Here, the light source 1 for absorption measurement, the light source 3 for LIF 3, the first 45-degree reflective notch filter 15, the light guide A, the light guide B, and the light guide included in the measurement light irradiation / absorption measurement unit 9. The optical system including C, light guide D, light guide E, and light absorption sensor 7 is, for example, a pigment-containing silicone resin 35 in which a black pigment made of carbon is dispersed in PDMS (an example of the “light absorption part” in the claims). Embedded in the unit casing 37. That is, an optical system for light absorption measurement comprising the light source 1 for light absorption measurement, the light guide A, the first 45-degree reflective notch filter 15, the light guide C, the sample holder 5, the light guide D, and the light absorption sensor 7. The LIF measurement light guide / fluorescence emission optical system comprising the LIF light source 3, the light guide B, the first 45-degree reflective notch filter 15, the light guide C, the sample holder 5, and the light guide E is a pigment. It is surrounded by the containing silicone resin 35 (unit casing 37).

  The pigment dispersed in the pigment-containing silicone resin 35 includes LIF excitation light 19 emitted from the LIF light source 3, autofluorescence generated when the sample holding unit 5 is irradiated with the LIF excitation light 19, and LIF When the excitation light 19 travels through the resin (light guide B, light guide C, light guide B ′), Raman light generated from the resin, or incident light 11 for absorption measurement from the light source 1 for absorption measurement The autofluorescence generated when the sample holder 5 is irradiated with the incident light 11 for absorbance measurement, the absorbance measurement light 27 that has passed through the sample in the sample holder 5, the incident light 11 for absorbance measurement, and the absorbance measurement light 27 are It has a wavelength characteristic that absorbs Raman light generated from the resin when traveling through the resin (the light guide A, the light guide C, and the light guide D).

  A light guide A, a light guide B, a light guide B ′, a light guide C, a light guide D, a light guide, which form the light guide of the optical system for absorption measurement and the measurement light guide / fluorescence emission optical system for LIF. If the material of the first silicone resin 12 constituting the optical part E and the material of the pigment-containing silicone resin 35 are the same, these refractive indexes are all the same. Therefore, there is no refractive index boundary at the interface between each light guide and the pigment-containing silicone resin 35 (unit housing 37) surrounding each light guide. Therefore, when stray light from each light guide portion enters the pigment-containing silicone resin 35, light reflection and light scattering of stray light at the interface are suppressed.

  By adopting the structure of this embodiment, as shown in FIG. 4, incident light from the light source for light absorption measurement 217 and the light source for LIF 219 to the sample is guided to the same light guide window (hereinafter referred to as a light guide window 239 for sharing incident light). It is also an example of “incident light shared light guiding window” in the claims of the present application. Therefore, in the light-absorbing part surrounding the sample holding part 205 made of a PCR tube, the places where the light guide window needs to be provided are the incident light shared light guide window 239, the LIF measurement light light guide window 233 (the “No. It is an example of “2 measurement light guide window”) and a measurement light guide window for light absorption 237 (an example of “first measurement light guide window” in the claims of this application). As a result, the light-shielding property of the sample holding unit 205 is improved, and it is possible to provide a small and portable composite sensor that can simultaneously perform light-induced fluorescence measurement and absorbance measurement.

  In this embodiment, stray light is incident on the pigment-containing silicone resin 35 (unit housing 37 of the measurement light irradiation / absorption measurement unit 9) and absorbed, and light reflection and light scattering of stray light are suppressed at the interface. By utilizing this, the light reaching the light absorption sensor 7 (part of the LIF excitation light 19 and the light absorption measurement light 27) absorbs the stray light scattered by the light receiving surface 39 of the light absorption sensor 7, and the fluorescence collection of this stray light This is to reduce noise by suppressing entry into an optical system 65 (described later).

  Concretely, it demonstrates using FIG. 5 which expanded the peripheral part (part enclosed by the dotted line) of the light absorption sensor 7 of FIG.

  In the present embodiment, the light absorption sensor 7 is installed such that the light receiving surface 39 of the light absorption sensor 7 is inclined with respect to the optical axis 41 of the light guide D. By disposing the light absorption sensor 7 in this way, a part of the light absorption measurement light 27 and the LIF excitation light 19 (hereinafter referred to as light absorption measurement surface incident light 43) incident on the light receiving surface 39 of the light absorption sensor 7 is transmitted along the optical axis. Reflected in a direction different from 41, enters the pigment-containing silicone resin 35 (unit housing 37) surrounding the light guide D, and is absorbed by the pigment. Further, the scattered light of the absorption measurement surface incident light 43 incident on the measurement surface 42 and scattered by the measurement surface 42 also travels in a direction different from the optical axis 41 and contains a pigment that surrounds the light guide D. It enters the silicone resin 35 (unit casing 37) and is absorbed by the pigment.

  If the distance between the sample holder 5 and the light absorption sensor 7 (the length of the light guide D) is secured to some extent, the effect of absorbing reflected / scattered light by the pigment-containing silicone resin 35 (unit housing 37) becomes remarkable. Of the scattered light, the light that coincides with the optical axis 41 of the light guide D is incident on the sample of the sample holder 5 again, but the intensity of the scattered light is significantly smaller than the intensity of the reflected light. Noise due to scattered light incident on the sample need not be considered much.

  In addition, the light 45 passing through the LIF excitation light 19 that has passed through the first 45-degree reflective notch filter 15 is incident on the light guide B ′ shown in FIG. 3. As described above, the first 45-degree reflective notch filter 15 reflects this light when the LIF excitation light 19 is incident at an incident angle of 45 degrees.

  As shown in FIG. 3, the optical axis of the LIF excitation light 19 emitted from the LIF light source 3 is set to be 45 degrees with respect to the incident surface of the first 45-degree reflective notch filter 15. Yes. Here, the first 45-degree reflection-type notch filter 15 has an incident angle dependency, and the LIF excitation light 19 incident at an incident angle of 45 ° is reflected by almost 100%, but the incident angle is from 45 °. When it is large or small, a part of the LIF excitation light 19 is not reflected but is transmitted as it is.

  Since the LIF excitation light 19 emitted from the LIF light source 3 has a spatial extent to some extent, the incident angle to the first 45-degree reflective notch filter 15 has a light component larger or smaller than 45 degrees. .

  A part of such light passes through the first 45 degree reflective notch filter 15 although it is reduced to some extent by the first 45 degree reflective notch filter 15. The intensity of the passing light 45 of the excitation light is sufficiently smaller than the intensity of the LIF excitation light 19 reflected by the first 45-degree reflective notch filter 15, but stray light enters the fluorescence collecting optical system and causes noise. It can also cause an outbreak.

  Here, the end of the light guide B ′ in the direction opposite to the first 45-degree reflective notch filter 15 side is in contact with the pigment-containing silicone resin 35 (unit housing 37). Therefore, the passing light 45 of the excitation light enters the pigment-containing silicone resin 35 and is absorbed (trapped) by the pigment. Therefore, the transmitted light 45 of the excitation light hardly enters the fluorescence collecting optical system.

  On the other hand, the fluorescence emitted from the sample of the sample holder 5 is emitted from the entire circumference of the sample holder 5. A part of the emitted light is collected by a fluorescence collecting optical system 65 described later. Other light becomes stray light in some cases. Therefore, it is preferable that the fluorescence 29 incident on the first dye-containing silicone resin 33 shown in FIGS. 2 and 3 shields components not collected by the fluorescence collection optical system 65 as much as possible. In order to cope with this, it is preferable that the light guide E through which the fluorescence passes has a shape that allows the light collected by the fluorescence collection optical system 65 to pass through as much as possible.

  The light guide E is surrounded by the pigment-containing silicone resin 35 (unit casing 37), and the first dye-containing silicone resin 33 is the same pigment-containing silicone resin as the unit casing 37 as described later. 35, the light that travels in a direction other than the light beam collected by the fluorescence collecting optical system 65 in the light guide E and the first dye-containing silicone resin 33 is a pigment. The light is incident on and absorbed by a unit casing 37 and a measuring instrument casing 71 made of the containing silicone resin 35.

  Further, since the light guide E is shaped so as to pass only the light beam collected by the fluorescence collecting optical system 65 as much as possible, as a result, the interface 73 between the light guide E and the first dye-containing silicone resin 33. , The end face area of the light guide E is smaller than the end face area of the first dye-containing silicone resin 33. That is, the interface 73 has an aperture structure (first aperture structure 75) that allows only the light beam collected by the fluorescence collecting optical system 65 to pass as much as possible.

  The end face shape of the light guide E at the interface 73 is, for example, a rectangle, and the direction perpendicular to the paper surface of FIG. 3 is the longitudinal direction of the rectangle.

(G) The fluorescence 29 passing through the light guide E made of the first silicone resin 12 that is transparent from the optical system on the output side of the measurement light irradiation / absorption measurement unit and the case is contained in the first dye shown in FIG. Silicone resin 33, first air chamber 47, second air chamber 49, second silicone resin 51, third air chamber 53, second 45-degree reflective notch filter 55, fourth air chamber 57, The fluorescence collecting optical system 65 including the fifth air chamber 59, the second dye-containing silicone resin 61, and the sixth air chamber 63 is an example of the LIF sensor 67 (an example of the “second measuring unit” in the claims). )) Is collected on the light receiving surface 69.

(G-1). The first dye-containing silicone resin The first dye-containing silicone resin 33 (an example of the “optical filter portion” in the claims of the present application) is made of a silicone resin (an example of the “second resin” in the claims of the application). The light guide E, the inclined surface 77 of the first air chamber 47, and the second air chamber 49.

  In addition, in order to remove light other than the fluorescence 29, the first dye-containing silicone resin 33 includes LIF excitation light 19, autofluorescence generated when the sample holder 5 is irradiated with the LIF excitation light 19, And the LIF excitation light 19 contains a pigment having a wavelength characteristic that absorbs Raman light generated from the resin as it travels through the resin (each light guide portion of the measurement light irradiation / absorption measurement unit 9). ing. As the 1st pigment | dye containing silicone resin 33, PDMS resin which diffused Sudan I pigment | dye is used, for example. However, it is better to use a silicone resin having a lower pigment concentration than the second pigment-containing silicone resin 61 for the first pigment-containing silicone resin 33. This is because even a dye that does not emit light does not emit light at all, and the dye may become a pseudo point light source.

(G-2). First air chamber
The first air chamber 47 is in contact with the first dye-containing silicone resin 33 at the inclined surface 77. In the first air chamber 47, due to the refractive index difference between the silicone resin and the atmosphere, the fluorescence emitted from the sample at an incident angle greater than the critical angle of the PDMS resin with respect to the atmosphere in the first air chamber 47 is the first air chamber. When incident on the inclined surface 77 of 47, the fluorescence is totally reflected on the inclined surface 77. Therefore, by appropriately setting the angle of the inclined surface 77 of the first air chamber 47, the fluorescence is folded back in an arbitrary direction (upward in FIG. 2).

(G-3). Second Air Chamber The second air chamber 49 is provided between the first dye-containing silicone resin 33 and the second silicone resin 51. The boundary surface 79 between the first dye-containing silicone resin 33 and the second air chamber 49 is a spherical surface convex toward the second air chamber 49, and the second air chamber 49 and the second silicone resin The boundary surface 81 with 51 is a flat surface. Therefore, the entire second air chamber 49 has a plano-concave lens shape. By appropriately setting the curvature of the boundary surface 79, the diffused light emitted from the sample can be adjusted to parallel light by the second air chamber 49.

(G-4). Second silicone resin The second silicone resin 51 includes a light emitting side boundary surface 81 of the second air chamber 49, a slope 85 of the third air chamber 53, a slope 87 of the fourth air chamber 57, and a fifth The light incident side boundary surface 89 of the air chamber 59 is in contact with the second to fifth air chambers, respectively. A second 45-degree reflective notch filter 55, which will be described later, is inserted in the middle of the light guide section from the slope 85 to the slope 87 constituted by the second silicone resin 51.

(G-5). Third Air Chamber The third air chamber 53 is in contact with the second silicone resin 51 at the inclined surface 85. Similar to the first air chamber 47, since there is a difference in refractive index between the atmosphere in the air chamber and the silicone resin, the second air can be set by appropriately setting the angle of the inclined surface 85 of the third air chamber 53. Fluorescence incident from the chamber 49 is folded in an arbitrary direction (left direction in FIG. 2).

(G-6). Second 45 degree reflection type notch filter The second 45 degree reflection type notch filter 55 transmits fluorescence and light in a wavelength region in the vicinity thereof, and has an incident angle of 45 degrees with respect to the light incident surface of the notch filter. Reflects incident light other than fluorescent light and light in the wavelength region in the vicinity thereof. That is, as shown in FIG. 2, the second 45-degree reflection type notch filter 55 is installed such that its light incident surface is 45 degrees with respect to the optical axis direction (horizontal direction in FIG. 2).

  Not only the fluorescence to be measured but also other light (stray light) is incident on the second silicone resin 51. The other light means LIF excitation light 19, autofluorescence generated when the sample holder 5 is irradiated with the LIF excitation light 19, and from the resin when the LIF excitation light 19 travels in the resin. The generated Raman light. When the stray light passes through the first dye-containing silicone resin 33, most of the stray light is absorbed by the dye.

  Therefore, the intensity of light (stray light) other than fluorescence incident on the second silicone resin 51 is considerably attenuated. When such stray light is incident on the light incident surface of the second 45-degree reflective notch filter 55 at an incident angle of 45 degrees, the light is transmitted by the second 45-degree reflective notch filter 55 (lower part of FIG. 2). And is incident on the pigment-containing silicone resin 35 (measuring device casing 71) and absorbed by the pigment.

  Similar to the first 45-degree reflective notch filter 15, the second 45-degree reflective notch filter 55 also has an incident angle dependency. Therefore, light other than fluorescence (stray light) incident at an incident angle of 45 ° is reflected by almost 100%, but when the incident angle is larger or smaller than 45 °, part of the light other than fluorescence is not reflected. It passes through as it is.

  Light other than fluorescence, including the LIF excitation light 19 or the like, has a spatial extent to some extent, so that the incident angle to the second 45-degree reflective notch filter 55 is larger or smaller than 45 degrees.

  A part of such light is attenuated to some extent by the second 45 degree reflection type notch filter 55, but a part of the light passes. However, most of the light other than fluorescence is absorbed by the first dye-containing silicone resin 33 and further attenuated to some extent when passing through the second 45-degree reflective notch filter 55. Light other than fluorescence that passes through the 45-degree reflective notch filter 55 is weak light.

(G-7). Fourth Air Chamber The fourth air chamber 57 is in contact with the first silicone resin at the inclined surface 87. As with the first and third air chambers 47 and 53, there is a difference in refractive index between the atmosphere in the air chamber and the silicone resin, so the angle of the slope 87 of the fourth air chamber 57 should be set appropriately. Thus, the fluorescence reflected from the interface 85 of the third air chamber 53 is folded back in an arbitrary direction (downward in FIG. 2).

(G-8). Fifth Air Chamber The fifth air chamber 59 is provided between the second silicone resin 51 and the second dye-containing silicone resin 61. The boundary surface 89 between the second silicone resin 51 and the fifth air chamber 59 is a flat surface, and the boundary surface 93 between the second dye-containing silicone resin 61 and the fifth air chamber 59 is the fifth air chamber 59. Convex spherical surface. Therefore, the fifth air chamber 59 as a whole has a plano-concave lens shape. Therefore, the fluorescence reflected by the slope 87 of the fourth air chamber 57 passes through the boundary surface 89 as parallel light as it is. By appropriately setting the curvature of the boundary surface 93, the parallel light is converged on the slope 95 of the sixth air chamber 63 facing the second dye-containing silicone resin 61, and finally the light receiving surface of the LIF sensor 67. Condensed at 69.

(G-9). Second Dye-Containing Silicone Resin The second dye-containing silicone resin 61 is in contact with the slope 95 of the sixth air chamber 63 and the light receiving surface 69 of the LIF sensor 67, and the first dye-containing silicone resin 33. Similarly to LIF, the LIF excitation light 19, autofluorescence generated when the sample holder 5 is irradiated with the LIF excitation light 19, and the LIF excitation light 19 are resin (each of the measurement light irradiation / absorption measurement unit 9). It contains substantially uniformly a dye having a wavelength characteristic that absorbs Raman light generated from the resin when traveling through the light part, the first dye-containing silicone resin 33, and the second silicone resin 51). In order to remove light other than fluorescence as much as possible, it is better to use a silicone resin having a higher pigment concentration than the first pigment-containing silicone resin 33 for the second pigment-containing silicone resin 61.

(G-10). Sixth Air Chamber The sixth air chamber 63 is in contact with the second dye-containing silicone resin 61 at the inclined surface 95. Similar to the first, third, and fourth air chambers, there is a difference in refractive index between the atmosphere in the air chamber and the silicone resin, so the angle of the slope 95 of the sixth air chamber 63 is appropriately set. By setting, the fluorescence reflected by the interface of the fourth air chamber 57 is folded in an arbitrary direction (left direction in FIG. 2). That is, the light that passes through the boundary surface 93 between the fifth air chamber 59 and the second dye-containing silicone resin 61 and converges on the slope 95 of the sixth air chamber 63 is collected on the light receiving surface 69 of the LIF sensor 67. Lighted.

(G-11). Measuring instrument housing
The fluorescence collecting optical system 65 from the first dye-containing silicone resin 33 to the LIF sensor 67 through which the light emitted from the measuring light irradiation / absorption measuring unit 9 passes is made by the pigment-containing silicone resin 35 (measuring instrument casing 71). Besieged. At least the light receiving surface 69 of the LIF sensor 67 is embedded in or in contact with the pigment-containing silicone resin 35 (measuring device casing 71). The pigment-containing silicone resin 35 is the same material as the first dye-containing silicone resin 33, the second silicone resin 51, and the second dye-containing silicone resin 61 that form the light guide, and for example, a PDMS resin is used. It is done. The pigment of the pigment-containing silicone resin 35 includes LIF excitation light 19 emitted from the LIF light source 3, autofluorescence generated when the sample holder 5 is irradiated with the excitation light 19, and excitation light 19 is a resin (first When traveling through the first dye-containing silicone resin 33, the second silicone resin 51, and the second dye-containing silicone resin 61), the Raman light generated from the resin is absorbed. For the pigment of the pigment-containing silicone resin 35, for example, a black pigment made of carbon is used. The measuring instrument housing 71 includes a silicone resin (a first dye-containing silicone resin 33, a second silicone resin 51, and a second dye-containing silicone resin 61) whose main constituent elements form a light guide portion of an optical system. Since these are silicone resins of the same material, these refractive indexes are all the same. That is, the silicone resin (the first dye-containing silicone resin 33, the second silicone resin 51, the second dye-containing silicone resin 61) that forms the light guide portion of the measuring instrument housing 71 and the fluorescence collecting optical system 65. There is no refractive index boundary between them. Therefore, when the stray light that has passed through the region occupied by the silicone resin that forms the light guide portion of the fluorescence collecting optical system 65 is incident on the measuring instrument casing 71, both silicone resins (the pigment-containing silicone that constitutes the measuring instrument casing 71). Since reflection and scattering of light are suppressed at the interface where the resin 35 and the silicone resin that forms the light guide part of the fluorescence collecting optical system 65 are in contact with each other, each stray light traveling through the light guide part is reflected and scattered. Without being incident on the measuring instrument casing, it is efficiently absorbed by the measuring instrument casing.

(G-12). Second aperture structure A second aperture structure 97 made of a member having an opening (for example, a slit shape) may be provided at a position near the light receiving surface 69 in the second dye-containing silicone resin 61. The second aperture structure 97 has a function of shielding light that is emitted from the sample of the sample holding unit 5 and travels other than the fluorescent light guide unit that reaches the light receiving surface 69 of the LIF sensor 67.

  The second aperture structure 97 is formed of the same silicone resin as the second dye-containing silicone resin 61 and contains the same pigment as the pigment-containing silicone resin 35. Therefore, since there is no refractive index boundary at the interface between the second dye-containing silicone resin 61 and the second aperture structure 97, when stray light enters the second aperture structure 97, reflection or scattering of light is suppressed. . Further, since the second aperture structure 97 contains a pigment, at least a part of the stray light is absorbed by the pigment of the second aperture structure 97.

  Thus, by providing the second aperture structure 97, the amount of stray light reaching the light receiving surface 69 of the LIF sensor 67 can be further reduced.

(H) LIF sensor As the LIF sensor 67, for example, a photomultiplier tube is used.

  6 and 7 show a configuration example of the second embodiment. In the present embodiment, the inclination direction of the light absorption sensor 7 is changed in the measurement light irradiation / absorption measurement unit 9 of the first embodiment. Other structures are the same as those in the first embodiment. Hereinafter, the inclination direction of the absorption sensor 7 in the measurement light irradiation / absorption measurement unit 9 will be described.

  In Example 1, as shown in FIG. 5, the inclination direction of the absorption sensor 7 in the measurement light irradiation / absorption measurement unit 9 is the absorption measurement light 27 passing through the light guide D and the LIF excitation light 19. The optical axis direction is set to be converted into the horizontal direction (the direction in the drawing). (In FIG. 5, the optical axis is converted to the left.)

  On the other hand, in Example 2, as shown in FIG. 7 showing a part of the AA cross-sectional view of FIG. Are set so as to be inclined with respect to a plane including the optical axis of the fluorescent light 29 and the optical axis of the fluorescence 29 without being orthogonal to each other.

  By setting the inclination angle of the light receiving surface 39 of the light absorption sensor 7 to 45 degrees with respect to the plane, a part of the absorption measurement light 27 reflected by the light receiving surface 39 and a part of the LIF excitation light 19 ( The absorption measurement surface incident light 43) is perpendicular to the paper surface of FIG. 6 and travels from the front side to the back side of the paper surface. That is, most of the stray light travels in this direction. On the other hand, since the central axis of the light guide E is on the paper surface of FIG. 6, according to the configuration of the second embodiment, the possibility of stray light traveling to the fluorescence collecting optical system 65 is reduced as compared with the first embodiment. It becomes possible.

  The light guide portion D made of the transparent first silicone resin 12 further includes a stray light trap portion 101 below the light receiving surface 39 of the light absorption sensor 7. Therefore, the reflected light or stray light generated on the light receiving surface 39 of the light absorption sensor 7 proceeds to the stray light trap portion 101, enters the pigment-containing silicone resin 35 constituting the unit housing 37, and is absorbed by the pigment.

  Next, Example 3 will be described with reference to FIGS. In Example 3, the function of the dye-containing silicone resin was easily maintained. FIG. 8 is an enlarged view of an optical filter portion including the dye diffusion suppressing member of the present invention, (a) an enlarged view around the first dye-containing silicone resin 33, and (b) a second dye-containing silicone resin. It is an enlarged view of 61 periphery. FIG. 9 is an enlarged view of an optical filter unit including the second light absorption unit of the present invention.

(1) Dye Diffusion Suppression Member Referring to FIG. 8, the dye contained in the first dye-containing silicone resin 33 and the second dye-containing silicone resin 61 is generally a dye and can move within the resin. is there. Therefore, depending on the case, the pigment-containing silicone resin 35 (measurement unit casing 71) surrounding the first pigment-containing silicone resin 33 and the second pigment-containing silicone resin 61 may ooze out. In that case, since the density | concentration of the pigment | dye contained in the 1st pigment | dye containing silicone resin 33 and the 2nd pigment | dye containing silicone resin 61 reduces, the absorption of the stray light in a pigment | dye containing silicone resin may become inadequate.

  Therefore, in Example 3, as shown in FIG. 8, the periphery of the first dye-containing silicone resin 33 and the second dye-containing silicone resin 61 was surrounded by the dye diffusion suppression member 103. In this case, there is a refractive index boundary at the boundary surface between the dye diffusion suppression member 103 and the first dye-containing silicone resin 33 and at the boundary surface between the dye diffusion suppression member 103 and the second dye-containing silicone resin 61. . However, since the stray light reflected / scattered at the refractive index boundary is absorbed by the dye-containing silicone resin (the first dye-containing silicone resin 33 and the second dye-containing silicone resin 61), the LIF sensor 67 substantially. Does not reach the light receiving surface 69.

  The dye diffusion suppressing member 103 is made of a material having a high density, a high transmittance with respect to desired light, and a small or negligible movement of the dye inside. Specifically, acrylic resins such as polymethyl methacrylate resin (PMMA), polyethylene terephthalate (PET), polycarbonate, inorganic glass, and the like are used. In particular, it is preferable to use inorganic glass as the dye diffusion suppressing member 103 if the movement of the dye is completely prevented.

(2) Second Absorbing Part Next, with reference to FIG. 9, between the dye diffusion suppressing member 103 and the first dye-containing silicone resin 33, and between the dye diffusion suppressing member 103 and the second dye-containing silicone resin. 61 may further include a second light absorption unit 105 that absorbs light having a wavelength different from that of the fluorescence to be measured. This makes it possible to suppress reflection / scattering in the optical filter unit while securing a light guide unit through which fluorescence as a measurement target travels. The second light absorbing portion 105 is composed of the same resin as the first dye-containing silicone resin 33 and the second dye-containing silicone resin 61. What are the dyes contained in these dye-containing silicone resins? A different second dye 107 may be included. Since the second light-absorbing part 105, the first dye-containing silicone resin 33, and the second dye-containing silicone resin 61 are the same material, they have the same refractive index and suppress the occurrence of reflection / scattering at the interface. It becomes even easier.

DESCRIPTION OF SYMBOLS 1 ... Light source for absorption measurement, 3 ... Light source for LIF, 5 ... Sample holding part, 7 ... Absorption sensor, 9 ... Measurement light irradiation and absorption measurement unit, 11 ... Absorption measurement Incident light, 12 ... first silicone resin, 15 ... first 45 degree reflection type notch filter, 19 ... excitation light for LIF, 27 ... absorption measurement light, 29 ... fluorescence 33... First dye-containing silicone resin, 35... Pigment-containing silicone resin, 37... Unit housing, 39. .. Absorption sensor light receiving surface incident light, 45... LIF excitation light 19 passing light, 47... First air chamber, 49... Second air chamber, 51. Resin, 53... Third air chamber, 55... Second 45 degree reflection type notch filter, 57. 4th air chamber, 59... 5th air chamber, 61... 2nd dye-containing silicone resin, 63... 6th air chamber, 65. LIF sensor, 69... Light receiving surface of LIF sensor 67, 71... Measuring instrument housing, 73... Interface between light guide E and first dye-containing silicone resin, 75. First aperture structure, 77... Slope of first air chamber 47, 79... Interface between first dye-containing silicone resin 33 and second air chamber 49, 81. The boundary surface between the air chamber 49 and the second silicone resin 51, 83... The second silicone resin 51 and the light emitting side boundary surface of the second air chamber 49, 85. The slope of the third air chamber 53, 87... The slope of the second silicone resin 51 and the fourth air chamber 57, 9... Light incident side interface between the second silicone resin 51 and the fifth air chamber 59, 91... Interface between the second silicone resin 51 and the fifth air chamber 59, 93. Interface between second dye-containing silicone resin 61 and fifth air chamber 59, 95... Slope of second dye-containing silicone resin 61 and sixth air chamber 63, 97. Structure: 99... Plane including the optical axis of the absorption measurement light 27 and the optical axis of the fluorescence 29, 101... Stray light trap part, 103. ... second dye, 201 ... light-induced fluorescence measuring device, 203 ... solid light source, 205 ... sample holder, 207 ... light emitting surface of solid light source 205, 209 ... fluorescence measurement 211, the light receiving surface of the fluorescence measuring device 209, 213, the housing, 21 5 ... PCR tube, 217 ... white light source for absorption measurement, 219 ... light source for LIF, 221 ... absorption sensor, 223 ... sensor for LIF, 225 ... fluorescence collection optical system, 227 ... transparent silicone resin, 229 ... silicone resin containing black pigment, 231 ... incident light guide window for LIF, 233 ... measurement light guide window for LIF, 235 ... incident light for light absorption Light window, 237 ... Measurement light guide window for light absorption, 239 ... Light guide window for incident light, A to E ... Light guide section

Claims (10)

The first light source, the first measurement unit that measures the light intensity of the first measurement light emitted from the first light source and transmitted through the measurement object, the second light source, and the light emitted from the second light source are irradiated. A second measuring unit for measuring second measuring light that is fluorescence from the measured object,
A light guide unit through which light passes until the light emitted from the first light source reaches the first measurement unit and the light emitted from the second light source reaches the second measurement unit;
A sample holder for holding the measurement object;
A light-absorbing part surrounding the light guide part and the sample holding part,
The light guide unit is transparent to the first incident light emitted from the first light source, the first measurement light, the second incident light emitted from the second light source, and the second measurement light. Made of resin,
The sample holder is
An incident light sharing light guide window for guiding the first incident light and the second incident light to the measurement object;
A first measurement light guide window for guiding the first measurement light from the measurement object to the first measurement unit;
An optical measurement device comprising: a second measurement light guide window for guiding the second measurement light from the measurement object to the second measurement unit.   The light measurement device according to claim 1, wherein the light receiving surface of the first measurement unit is inclined without being orthogonal to the optical axis of the first measurement light.   3. The optical measurement apparatus according to claim 2, wherein the light receiving surface is inclined without being orthogonal to a plane including the optical axis of the second incident light and the optical axis of the second measurement light. .   A first optical member that makes the optical axis of the second incident light parallel to the optical axis of the first incident light is further provided between the second light source and the sample holder of the light guide. Item 4. The light measurement device according to any one of Items 1 to 3. A second optical member that shields light having a wavelength different from that of the second measurement light between the sample holding unit and the second measurement unit;
The optical measurement apparatus according to claim 4, wherein the first optical member and the second optical member have optical characteristics that allow the first incident light to pass and reflect the second incident light.   The optical measurement device according to claim 1, further comprising an optical filter unit made of a second resin having a pigment between the sample holding unit and the second measurement unit in the light guide unit. .   The light measurement device according to claim 6, further comprising a dye diffusion suppression unit around the optical filter unit.   The optical measurement device according to claim 7, further comprising a second light absorption unit that absorbs light having a wavelength different from that of the second measurement light between the optical filter unit and the dye diffusion suppression unit.   The optical measurement apparatus according to claim 8, wherein the second light absorption part is made of the same resin as the second resin constituting the optical filter part, and includes a second dye different from the dye. The first light source, the first measurement unit that measures the light intensity of the first measurement light emitted from the first light source and transmitted through the measurement object, the second light source, and the light emitted from the second light source are irradiated. A light measurement method in a light measurement device comprising: a second measurement unit that measures second measurement light that is fluorescence from the measured object,
The light measuring device comprises:
A light guide unit through which light passes until the light emitted from the first light source reaches the first measurement unit and the light emitted from the second light source reaches the second measurement unit;
A sample holder for holding the measurement object;
A light-absorbing part surrounding the light guide part and the sample holding part,
The light guide unit is transparent to the first incident light emitted from the first light source, the first measurement light, the second incident light emitted from the second light source, and the second measurement light. Made of resin,
The sample holder is
An incident light sharing light guide window for guiding the first incident light and the second incident light to the measurement object;
A first measurement light guide window for guiding the first measurement light from the measurement object to the first measurement unit;
A second measurement light guide window for guiding the second measurement light from the measurement object to the second measurement unit;
The light measurement method includes an irradiation step in which the first light source and the second light source irradiate the measurement target with the first incident light and the second incident light, respectively, through the incident light shared light guiding window.